Standard name
Human Ortholog
Description Protein required for function of the Sit4p protein phosphatase; forms a complex with Sit4p; member of a family of similar proteins including Sap4p, Sap185p, and Sap190p; protein abundance increases in response to DNA replication stress; SAP155 has a paralog, SAP4, that arose from the whole genome duplication
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0.05 0 0 0 0.06 0.05 0 0 0.05 0 0 0.09 0.05 0 0 0 0 0 0
Cytoplasm 0.98 0.99 0.97 0.97 0.97 0.94 0.9 0.86 0.83 0.74 0.76 0.79 0.98 1.0 0.95 0.92 0.93 0.93 0.95 0.94 0.92 0.83 0.84 0.89
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0 0 0 0 0 0 0.08 0.09 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0.08 0.06 0.1 0.18 0.25 0.28 0.23 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.08 0.05 0.06 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 1 1 0 2 0 3 0 0 0 0 0 1 0 0 0 0 1 0 0 2 1
Bud 3 1 1 0 1 0 0 1 1 1 2 2 0 0 6 0 1 0 0 0 0 1 0 2
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 6 9 5 3 8 9 6 6 3 6 6 2 8 17 3 12 38 18 0 0 0 0 0 0
Cytoplasm 202 395 167 105 215 181 161 151 72 80 97 90 321 360 371 404 395 351 187 390 171 178 351 333
Endoplasmic Reticulum 0 4 2 0 2 4 8 7 1 6 5 4 5 0 2 17 13 10 1 1 1 17 36 13
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 3 2 1 1 1
Golgi 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 1 2 0 4 0 0 0 0
Mitochondria 2 0 0 1 0 16 11 18 16 27 36 26 3 0 4 5 3 2 1 2 1 2 5 1
Nucleus 1 1 1 1 2 3 0 0 0 0 0 2 3 1 5 6 6 7 0 1 0 0 0 2
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0
Vac/Vac Membrane 2 0 0 1 1 1 1 7 1 4 2 3 0 0 12 34 22 22 1 5 2 8 10 10
Unique Cell Count 206 400 172 108 221 193 178 175 87 108 127 114 328 361 390 437 423 379 197 415 185 216 417 374
Labelled Cell Count 216 410 177 112 230 215 189 190 97 124 148 129 341 378 404 478 479 412 197 415 185 216 417 374


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 10.0 9.1 9.0 8.4 9.3 7.6 7.9 7.9 6.9 6.8 6.2 6.8 10.1 10.7 9.5 12.6 13.4 12.3 9.2 10.1 10.5
Std Deviation (1e-4) 1.6 1.3 1.3 1.2 1.3 1.4 1.4 1.6 1.5 1.5 1.3 1.3 1.3 1.4 2.4 2.8 2.6 2.6 1.5 1.5 1.6
Intensity Change (Log2) – – – -0.1 0.05 -0.24 -0.18 -0.18 -0.37 -0.41 -0.54 -0.41 0.17 0.25 0.08 0.48 0.58 0.45 0.04 0.17 0.23


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0.4 0.9 0.2 0.3 0 0 0 0 0 1.0 0 0 2.6 1.0
Cytoplasm 0.1 0.1 -1.5 -2.6 -3.6 -4.1 -5.8 -5.5 -5.0 0.5 2.7 -1.1 -2.1 -1.8 -2.1
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 1.7 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 3.9 3.3 4.3 5.8 6.9 7.4 6.6 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 3.8 3.0 3.2

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 6.11 7.7285 6.7113 6.3315 6.4986 6.9134 4.85 6.8336 6.1148 5.5521 5.449 6.0951 5.0983 6.6834 6.0041 5.5103 5.7162 5.9496
Actin 0.0257 0.0005 0.0026 0.0935 0.0233 0.0051 0.0175 0.0008 0.0091 0.0015 0.0302 0.0002 0.0016 0.0064 0.0099 0.0135 0.0232 0.0034
Bud 0.0006 0.0001 0.0004 0.0054 0.0002 0.0008 0.0013 0.0057 0.0003 0.0421 0.0025 0.0001 0.0009 0.014 0.013 0.0013 0.0061 0.0031
Bud Neck 0.0043 0.0003 0.0007 0.0033 0.0007 0.0069 0.0028 0.0002 0.0004 0.0007 0.0009 0.0007 0.0048 0.0003 0.0026 0.0009 0.0046 0.0011
Bud Periphery 0.0008 0.0001 0.0006 0.0029 0.0001 0.0006 0.0023 0.0024 0.0003 0.019 0.0012 0 0.0007 0.0051 0.0071 0.0011 0.0034 0.0018
Bud Site 0.0013 0.0028 0.0059 0.009 0.0005 0.0026 0.0063 0.0038 0.0011 0.0031 0.0005 0 0.0041 0.029 0.014 0.0101 0.0021 0.0007
Cell Periphery 0.0018 0.0008 0.0022 0.0008 0.0001 0.0002 0.0009 0.0002 0.0002 0.0005 0.0003 0 0.0002 0.0004 0.0003 0.0002 0.0002 0.0001
Cytoplasm 0.8149 0.9648 0.8997 0.7594 0.8271 0.9398 0.8403 0.9685 0.924 0.8251 0.883 0.9646 0.9208 0.9116 0.8977 0.9332 0.9005 0.9427
Cytoplasmic Foci 0.0226 0.0032 0.0177 0.0317 0.023 0.0077 0.0187 0.0045 0.009 0.0157 0.0091 0.007 0.0121 0.0107 0.0131 0.0121 0.0135 0.0121
Eisosomes 0.0002 0.0001 0.0002 0.0004 0.0001 0 0.0003 0 0.0003 0 0.0002 0 0 0 0.0001 0 0 0
Endoplasmic Reticulum 0.0203 0.0031 0.0087 0.0165 0.0037 0.0137 0.013 0.0015 0.0053 0.0141 0.0029 0.0094 0.0187 0.0013 0.0037 0.003 0.004 0.0069
Endosome 0.0236 0.0016 0.0113 0.0152 0.0322 0.0092 0.0287 0.0052 0.018 0.0421 0.0268 0.0122 0.0123 0.0049 0.0103 0.0115 0.0268 0.0132
Golgi 0.0061 0.0003 0.0038 0.0084 0.007 0.0023 0.0068 0.0006 0.003 0.0006 0.0173 0.0005 0.0011 0.0005 0.002 0.0012 0.0006 0.0009
Lipid Particles 0.0086 0.0001 0.0048 0.0054 0.0147 0.001 0.0193 0 0.012 0.0001 0.0085 0 0.0003 0.0001 0.0051 0.0006 0.0001 0.0002
Mitochondria 0.0018 0.0002 0.0098 0.0012 0.0015 0.0018 0.0068 0.0023 0.0075 0.0005 0.0025 0.0006 0.0004 0.0006 0.0018 0.0002 0.0003 0.0007
None 0.0355 0.0202 0.0194 0.0136 0.0486 0.0019 0.0061 0.0013 0.0013 0.004 0.0025 0.0012 0.0106 0.0097 0.0074 0.0032 0.0057 0.0068
Nuclear Periphery 0.004 0.0003 0.0011 0.0029 0.0011 0.0004 0.0053 0.0002 0.0007 0.0022 0.0015 0.0005 0.0015 0.0004 0.0017 0.0008 0.001 0.0007
Nucleolus 0.0023 0 0.0005 0.0003 0.0002 0 0.0006 0 0 0.0001 0.0001 0 0.0007 0.0002 0.0001 0.0001 0 0.0001
Nucleus 0.0033 0.0005 0.0021 0.0018 0.0007 0.0006 0.0022 0.0006 0.0006 0.0023 0.001 0.0008 0.0023 0.0014 0.002 0.0012 0.0014 0.0017
Peroxisomes 0.0104 0 0.0028 0.0149 0.0115 0.0033 0.009 0.0001 0.003 0.0001 0.0009 0 0.0002 0.0002 0.0042 0.0003 0.0005 0.0002
Punctate Nuclear 0.0046 0.0001 0.0019 0.0066 0.0016 0.0001 0.0018 0 0.0003 0.0006 0.0008 0.0001 0.003 0.0005 0.0012 0.0006 0.0008 0.0011
Vacuole 0.0067 0.0011 0.0034 0.006 0.0016 0.0017 0.0079 0.0017 0.0029 0.022 0.0055 0.0015 0.0033 0.0024 0.0021 0.0039 0.0045 0.0018
Vacuole Periphery 0.0007 0.0001 0.0004 0.0008 0.0006 0.0004 0.002 0.0004 0.0007 0.0038 0.0016 0.0005 0.0004 0.0003 0.0005 0.0007 0.0007 0.0004

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 22.3688 19.0129 19.4599 30.8858 26.3872 27.4874 33.0618 37.6942 32.229 36.5544
Translational Efficiency 0.8817 0.7969 0.8411 0.6263 0.5873 0.7213 0.6134 0.5184 0.6566 0.56

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1127 1361 1533 1337 1546 1814 1131 606 2673 3175 2664 1943

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 952.01 984.68 1396.60 1225.57 911.18 1018.47 1191.57 1212.64 928.39 1003.99 1309.55 1221.54
Standard Deviation 117.10 167.73 178.47 165.12 174.61 271.61 183.46 208.19 154.34 233.42 207.10 179.76
Intensity Change Log 2 — 0.048678 0.552870 0.364404 — 0.160595 0.387056 0.412343 — 0.104496 0.474158 0.388048

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000148 0.000204 0.000372 0.000281 0.000163 0.000361 0.000627 0.000317 0.000157 0.000294 0.000480 0.000292
Bud Neck 0.001850 0.001471 0.000665 0.001223 0.002310 0.002048 0.001492 0.001296 0.002116 0.001801 0.001016 0.001246
Bud Site 0.013723 0.019300 0.011268 0.019927 0.014330 0.023109 0.014702 0.023954 0.014074 0.021476 0.012726 0.021183
Cell Periphery 0.000146 0.000096 0.000149 0.000145 0.000136 0.000143 0.000076 0.000144 0.000140 0.000123 0.000118 0.000145
Cytoplasm 0.705170* 0.761506* 0.913371* 0.865515* 0.718981* 0.749254* 0.693597* 0.785550* 0.713158* 0.754506* 0.820066* 0.840574*
Cytoplasmic Foci 0.169570 0.124497 0.026946 0.031062 0.156214 0.142311 0.066093 0.056526 0.161845 0.134675 0.043566 0.039004
Eisosomes 0.000023 0.000011 0.000003 0.000004 0.000021 0.000016 0.000012 0.000005 0.000022 0.000014 0.000007 0.000005
Endoplasmic Reticulum 0.005965 0.002011 0.000904 0.000152 0.002803 0.000676 0.000708 0.000095 0.004136 0.001248 0.000821 0.000135
Endosome 0.012953 0.011628 0.001556 0.002668 0.013110 0.011212 0.008834 0.004752 0.013043 0.011390 0.004646 0.003318
Golgi 0.005944 0.006353 0.001321 0.003795 0.007038 0.011031 0.001380 0.000670 0.006577 0.009026 0.001346 0.002820
Lipid Particles 0.001707 0.000982 0.001543 0.000830 0.001548 0.000697 0.001048 0.001640 0.001615 0.000819 0.001333 0.001083
Mitochondria 0.000543 0.000564 0.000032 0.000469 0.000753 0.002487 0.000115 0.000021 0.000665 0.001663 0.000067 0.000329
Mitotic Spindle 0.001128 0.001578 0.001581 0.008538 0.001279 0.003459 0.002905 0.001323 0.001215 0.002653 0.002143 0.006287
None 0.001520 0.001227 0.000887 0.000214 0.005264 0.002088 0.000449 0.000201 0.003686 0.001719 0.000701 0.000210
Nuclear Periphery 0.000051 0.000040 0.000043 0.000031 0.000122 0.000029 0.000096 0.000027 0.000092 0.000034 0.000066 0.000030
Nuclear Periphery Foci 0.000574 0.000692 0.002287 0.000830 0.001165 0.000315 0.002105 0.001017 0.000916 0.000477 0.002209 0.000888
Nucleolus 0.000446 0.000452 0.000048 0.000337 0.000431 0.000152 0.000123 0.000095 0.000437 0.000281 0.000080 0.000262
Nucleus 0.008376 0.006470 0.012677 0.005073 0.013744 0.007334 0.011535 0.004614 0.011481 0.006964 0.012192 0.004930
Peroxisomes 0.000627 0.000881 0.000166 0.000761 0.000735 0.001764 0.000632 0.000885 0.000689 0.001386 0.000364 0.000800
Vacuole 0.065426 0.055765 0.023724 0.055547 0.055878 0.038151 0.192503 0.115450 0.059904 0.045702 0.095379 0.074230
Vacuole Periphery 0.004110 0.004271 0.000456 0.002599 0.003976 0.003361 0.000969 0.001419 0.004033 0.003751 0.000674 0.002231

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -1.48 -5.44 -3.73 -2.63 2.60 -3.18 -1.17 -3.29 0.22 0.70 -3.50 -2.04 -5.16 -0.37 1.14
Bud Neck 1.68 5.85 2.51 0.76 -3.19 0.66 2.39 3.13 3.10 1.32 1.36 5.29 3.92 2.93 -1.55
Bud Site -3.10 0.96 -2.36 0.30 -3.22 -5.71 0.20 -3.16 0.20 -3.18 -6.35 1.06 -3.72 0.99 -4.35
Cell Periphery 4.08 -0.64 0.32 -1.71 0.69 -1.13 7.15 -0.36 0.69 -4.63 0.75 2.27 0.08 -0.38 -1.05
Cytoplasm -5.81 -24.29 -16.77 -10.96 7.93 -3.48 4.22 -3.97 -1.36 -7.03 -6.41 -14.25 -16.98 -11.02 -2.84
Cytoplasmic Foci 7.06 26.91 26.19 21.09 -1.59 2.42 19.61 19.70 17.52 1.98 6.40 34.19 35.00 29.79 1.93
Eisosomes 6.43 10.96 10.39 7.65 -1.89 2.64 5.91 11.27 9.55 6.30 6.19 13.13 15.70 12.55 4.65
Endoplasmic Reticulum 2.64 3.43 3.99 5.25 2.92 3.66 3.93 4.81 4.16 4.65 4.02 4.76 5.75 6.44 4.03
Endosome 1.18 13.75 11.94 9.96 -1.69 1.77 6.52 9.94 6.99 3.99 2.12 15.08 16.21 12.42 2.72
Golgi -0.47 15.41 3.43 3.03 -2.01 -4.10 11.91 17.93 11.55 1.09 -3.72 18.75 7.06 8.14 -1.78
Lipid Particles 3.82 -0.08 3.45 -0.55 3.96 6.47 3.26 -0.79 -2.76 -1.83 7.22 1.19 1.74 -3.15 0.84
Mitochondria -0.08 4.62 0.92 0.78 -2.00 -2.05 2.44 2.70 3.08 2.03 -2.01 3.71 2.05 2.93 -1.75
Mitotic Spindle -0.50 -1.20 -3.30 -2.97 -2.89 -2.04 -1.53 0.13 2.10 1.60 -1.99 -1.91 -3.24 -2.07 -2.49
None 0.66 2.19 4.45 3.02 7.77 2.27 3.98 4.18 3.05 2.39 2.37 4.22 4.89 3.93 7.42
Nuclear Periphery 0.94 -0.64 1.37 0.48 3.02 1.86 0.10 1.74 -0.82 8.87 1.99 0.32 1.94 -0.23 8.09
Nuclear Periphery Foci -1.13 -9.83 -4.33 -2.92 7.70 4.21 -4.25 -0.09 -8.02 5.49 3.50 -8.72 -1.10 -7.98 9.61
Nucleolus 0.01 1.30 0.50 0.56 -1.36 4.54 6.07 6.68 1.73 1.85 0.90 2.68 1.24 0.39 -1.22
Nucleus 2.14 -8.49 3.86 0.61 14.07 3.41 0.26 6.13 2.84 9.61 3.96 -2.67 6.72 2.46 16.38
Peroxisomes -2.41 7.00 -0.91 1.29 -5.90 -4.21 1.47 -1.03 3.74 -2.32 -4.74 5.82 -0.87 4.07 -5.43
Vacuole 2.20 9.65 -1.28 -3.83 -12.68 6.40 -22.48 -10.11 -12.43 7.08 5.88 -12.37 -8.81 -13.21 3.45
Vacuole Periphery -0.27 11.82 2.93 2.97 -2.45 1.24 7.53 6.60 5.02 -1.28 0.84 13.05 4.46 3.78 -2.58
Standard name
Human Ortholog
Description Protein required for function of the Sit4p protein phosphatase; forms a complex with Sit4p; member of a family of similar proteins including Sap4p, Sap185p, and Sap190p; protein abundance increases in response to DNA replication stress; SAP155 has a paralog, SAP4, that arose from the whole genome duplication
Localization
Cell Percentages cytoplasm (100%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-4

Sap155

Sap155


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Sap155-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available