Standard name
Human Ortholog
Description Quinolinate phosphoribosyl transferase; required for the de novo biosynthesis of NAD from tryptophan via kynurenine; expression regulated by Hst1p
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Cytoplasm 0.98 0.98 0.99 0.94 0.95 0.92 0.86 0.79 0.67 0.73 0.72 0.68 0.97 0.97 0.97 – 0.93 0.94 0.98 0.95 0.95 0.96 0.92 0.89
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0.05 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0.05 0.05 0.27 0.18 0.28 0.23 0 0 0 – 0 0.05 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0.06 0 0 0.11 0.16 0.17 0.12 0.07 0.09 0 0 0 – 0 0.06 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 2 1 0 0 1 2 1 0 0 0 – 0 0 0 0 0 0 0 1
Bud 1 3 0 0 1 0 1 3 4 4 2 5 1 2 1 – 2 1 0 2 0 1 0 2
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 1 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 – – – – – –
Cell Periphery 3 5 0 1 5 7 9 9 4 5 6 3 2 1 3 – 3 3 0 0 0 0 0 0
Cytoplasm 237 391 142 144 229 183 307 320 191 223 146 154 262 413 463 – 242 247 233 386 152 280 275 263
Endoplasmic Reticulum 1 1 0 2 9 5 6 4 5 6 2 4 2 1 1 – 10 14 0 1 0 1 3 2
Endosome 0 0 0 0 0 0 0 0 0 1 1 1 1 0 0 – 4 0 0 0 1 1 1 5
Golgi 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 1 0 0 1 0
Mitochondria 1 3 0 2 0 4 17 20 76 54 56 52 0 0 0 – 11 12 1 2 1 0 1 3
Nucleus 2 3 0 1 0 1 8 10 3 6 3 10 0 1 1 – 3 1 0 1 0 0 3 2
Nuclear Periphery 0 1 0 0 0 0 0 0 1 0 0 2 0 0 0 – 0 0 0 0 0 0 1 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 1 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
SpindlePole 0 1 0 0 0 0 0 1 0 0 0 0 0 0 0 – 2 0 0 0 0 0 0 0
Vac/Vac Membrane 1 1 2 9 4 4 41 63 49 38 14 21 6 16 20 – 11 15 1 7 0 1 4 5
Unique Cell Count 241 397 143 153 240 198 358 406 283 306 202 226 269 426 475 261 262 239 405 160 291 300 296
Labelled Cell Count 247 409 144 159 248 206 390 430 333 338 232 253 274 434 489 289 294 239 405 160 291 300 296


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 12.2 8.3 11.0 10.6 10.6 8.6 8.2 8.4 6.8 6.7 6.4 6.7 11.4 11.6 11.6 4.0 4.9 5.3 10.8 10.7 11.2
Std Deviation (1e-4) 2.5 1.8 2.0 2.4 2.1 1.7 1.8 1.8 1.7 1.5 1.5 1.4 2.9 2.8 2.7 1.2 1.6 2.0 2.3 2.4 2.5
Intensity Change (Log2) – – – -0.05 -0.05 -0.34 -0.42 -0.39 -0.69 -0.7 -0.78 -0.72 0.06 0.08 0.08 -1.45 -1.15 -1.04 -0.02 -0.04 0.03


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0
Cytoplasm -2.5 -2.1 -3.0 -4.5 -5.8 -7.5 -6.7 -6.7 -7.3 -1.3 -1.6 -1.3 – -2.9 -2.5
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0
Mitochondria 0 0 0 0 2.7 6.8 5.4 6.9 6.2 0 0 0 – 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0
Vacuole 2.0 0 0 3.6 4.5 4.8 3.8 2.4 3.1 0 0 1.6 – 0 2.1

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 9.468 10.9743 10.404 11.0385 9.434 10.6973 6.9168 10.0047 8.5114 9.3688 8.4528 9.4625 5.305 6.9971 6.1844 6.7878 5.459 6.1459
Actin 0.0204 0.0011 0.0223 0.0009 0.01 0.0032 0.0235 0.0009 0.0109 0.0168 0.0042 0.0103 0.0147 0.0002 0.0062 0.0004 0.0006 0.0002
Bud 0.0011 0.0073 0.0006 0.0008 0.0017 0.0005 0.0018 0.0003 0.0009 0.0014 0.0022 0.0002 0.0003 0.0001 0.0007 0.0001 0.0016 0.0001
Bud Neck 0.0026 0.0004 0.0024 0.0006 0.001 0.002 0.0024 0.0002 0.0004 0.001 0.0005 0.0009 0.0006 0.0002 0.0003 0.0004 0.0007 0.0009
Bud Periphery 0.0009 0.0008 0.0005 0.0004 0.0029 0.0002 0.0022 0.0001 0.0009 0.0005 0.0034 0.0001 0.0002 0 0.0004 0 0.0005 0
Bud Site 0.0046 0.0088 0.0061 0.0002 0.0008 0.0003 0.0019 0.0005 0.001 0.0006 0.0025 0.0002 0.0011 0.0003 0.0006 0.0001 0.0006 0
Cell Periphery 0.0003 0.0002 0.0004 0.0001 0.0001 0 0.0003 0.0001 0.0001 0.0001 0.0002 0 0 0 0.0001 0 0 0
Cytoplasm 0.8236 0.9516 0.9044 0.9701 0.8132 0.9254 0.8087 0.8802 0.8804 0.8623 0.8523 0.94 0.8916 0.9865 0.9526 0.9757 0.8964 0.9651
Cytoplasmic Foci 0.0217 0.0077 0.0064 0.0035 0.0585 0.0074 0.0133 0.0062 0.0118 0.0309 0.0131 0.0057 0.018 0.002 0.0096 0.0029 0.0315 0.0033
Eisosomes 0.0001 0 0.0002 0 0.0001 0.0001 0.0004 0 0.0001 0 0 0 0.0001 0 0 0 0 0
Endoplasmic Reticulum 0.0066 0.0011 0.0029 0.0018 0.0069 0.0036 0.0046 0.0031 0.0069 0.001 0.0118 0.0033 0.0044 0.0007 0.0035 0.0015 0.0009 0.0015
Endosome 0.0252 0.0017 0.0031 0.0016 0.0233 0.0084 0.0158 0.0176 0.0142 0.0144 0.0326 0.005 0.0125 0.0008 0.0025 0.0012 0.0084 0.0017
Golgi 0.0077 0.0003 0.0022 0.0002 0.0061 0.0014 0.0053 0.0016 0.0102 0.0299 0.0099 0.0006 0.0025 0.0001 0.0006 0.0001 0.0017 0.0001
Lipid Particles 0.0029 0 0.0043 0 0.0091 0.0011 0.0069 0.0017 0.0024 0.0036 0.0067 0.0002 0.0025 0 0.0004 0 0.0001 0
Mitochondria 0.0054 0.0003 0.0036 0.0002 0.0024 0.0062 0.0109 0.0003 0.003 0.0031 0.0204 0.0011 0.0018 0.0001 0.0003 0.0001 0.0035 0.0001
None 0.0407 0.0038 0.0116 0.0041 0.0147 0.0105 0.0513 0.0672 0.0211 0.0042 0.0287 0.0182 0.0174 0.003 0.0068 0.0021 0.0349 0.0035
Nuclear Periphery 0.0058 0.0014 0.0016 0.0022 0.0032 0.0059 0.0147 0.0035 0.0104 0.0014 0.0019 0.0015 0.0035 0.0005 0.0019 0.0021 0.0012 0.0017
Nucleolus 0.0002 0.0001 0.0001 0 0.0003 0.0001 0.0003 0 0.0002 0 0.0001 0 0.0002 0 0 0 0.0001 0
Nucleus 0.0165 0.0087 0.0177 0.0109 0.006 0.0196 0.0094 0.0135 0.006 0.0101 0.0031 0.0109 0.01 0.0048 0.01 0.0123 0.0059 0.0207
Peroxisomes 0.0064 0.0002 0.0075 0.0001 0.0169 0.0007 0.0186 0.0003 0.0112 0.0171 0.0006 0.0002 0.0074 0 0.002 0 0.0088 0
Punctate Nuclear 0.003 0.0037 0.0009 0.0009 0.0202 0.0018 0.0025 0.0019 0.0065 0.0008 0.0009 0.0008 0.0098 0.0002 0.0006 0.0006 0.0007 0.0003
Vacuole 0.003 0.0007 0.0008 0.001 0.0021 0.0011 0.0031 0.0005 0.001 0.0004 0.0034 0.0006 0.0012 0.0003 0.0007 0.0003 0.0014 0.0006
Vacuole Periphery 0.0012 0.0001 0.0002 0.0002 0.0005 0.0006 0.0022 0.0002 0.0004 0.0002 0.0015 0.0002 0.0003 0 0.0001 0.0001 0.0005 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 31.6856 15.551 14.8365 21.3779 20.1381 39.7403 25.8316 23.4988 35.6115 25.8961
Translational Efficiency 0.9008 1.7237 1.3335 1.2418 1.3254 0.9323 1.4442 1.4371 0.9815 1.1185

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1954 366 2233 1659 1644 1304 237 1521 3598 1670 2470 3180

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 954.52 1136.16 1348.30 962.66 904.68 1120.06 1327.37 991.62 931.75 1123.59 1346.29 976.51
Standard Deviation 148.25 217.06 204.97 282.74 146.16 182.26 185.91 169.87 149.38 190.55 203.31 236.04
Intensity Change Log 2 — 0.251319 0.498294 0.012251 — 0.308097 0.553091 0.132380 — 0.279226 0.525218 0.071955

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000205 0.000315 0.000143 0.000765 0.000165 0.000194 0.000696 0.001045 0.000186 0.000220 0.000196 0.000899
Bud Neck 0.001690 0.001780 0.001055 0.006171 0.001180 0.002218 0.003378 0.003942 0.001457 0.002122 0.001278 0.005105
Bud Site 0.005266 0.013757 0.002337 0.020871 0.004659 0.007821 0.009595 0.025908 0.004989 0.009122 0.003033 0.023280
Cell Periphery 0.000075 0.000106 0.000049 0.000214 0.000068 0.000049 0.000256 0.000116 0.000072 0.000061 0.000069 0.000167
Cytoplasm 0.748422* 0.779654* 0.776919* 0.566214* 0.809948* 0.828524* 0.512557* 0.640890* 0.776535* 0.817813* 0.751553* 0.601932*
Cytoplasmic Foci 0.118369 0.112898 0.006942 0.026948 0.085073 0.072875 0.032638 0.023517 0.103155 0.081646 0.009408 0.025307
Eisosomes 0.000024 0.000014 0.000003 0.000044 0.000012 0.000010 0.000050 0.000025 0.000018 0.000011 0.000008 0.000035
Endoplasmic Reticulum 0.001848 0.001162 0.000379 0.001902 0.001680 0.000491 0.003672 0.001239 0.001771 0.000638 0.000695 0.001585
Endosome 0.005402 0.004330 0.000507 0.007813 0.003138 0.003413 0.011234 0.008528 0.004367 0.003614 0.001536 0.008155
Golgi 0.002508 0.001184 0.000128 0.005297 0.001721 0.002084 0.000421 0.007833 0.002148 0.001886 0.000156 0.006510
Lipid Particles 0.002045 0.000947 0.000414 0.001551 0.000974 0.000742 0.004118 0.002301 0.001556 0.000787 0.000769 0.001910
Mitochondria 0.001526 0.000086 0.000216 0.002494 0.000315 0.000556 0.000111 0.002309 0.000973 0.000453 0.000205 0.002405
Mitotic Spindle 0.002134 0.002166 0.006740 0.043050 0.001576 0.002343 0.002974 0.042295 0.001879 0.002304 0.006379 0.042689
None 0.002070 0.001940 0.007536 0.008661 0.003575 0.005005 0.002281 0.010098 0.002758 0.004333 0.007032 0.009348
Nuclear Periphery 0.000184 0.000110 0.000504 0.001130 0.000105 0.000077 0.001035 0.000817 0.000148 0.000084 0.000555 0.000980
Nuclear Periphery Foci 0.001738 0.003604 0.001829 0.002749 0.002124 0.000967 0.020242 0.002586 0.001914 0.001545 0.003595 0.002671
Nucleolus 0.000294 0.000197 0.000141 0.000708 0.000180 0.000215 0.000339 0.000568 0.000242 0.000211 0.000160 0.000641
Nucleus 0.056308 0.038282 0.172872* 0.138511* 0.047678 0.038394 0.095813 0.087599 0.052365 0.038369 0.165478* 0.114160
Peroxisomes 0.000606 0.001729 0.000055 0.001768 0.000441 0.000860 0.000308 0.001877 0.000530 0.001050 0.000079 0.001820
Vacuole 0.048352 0.035321 0.021141 0.158693 0.034544 0.032522 0.297893* 0.129452 0.042043 0.033136 0.047696 0.144707
Vacuole Periphery 0.000935 0.000418 0.000091 0.004445 0.000845 0.000640 0.000390 0.007056 0.000893 0.000591 0.000120 0.005694

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -2.78 -0.64 -2.44 -1.25 -2.31 -0.68 -9.34 -2.24 -1.99 1.70 -1.12 -3.78 -3.23 -2.64 -2.28
Bud Neck -0.37 -0.60 -5.04 -4.61 -4.86 -3.11 -9.10 -7.78 -3.32 1.98 -2.50 -4.61 -8.06 -4.86 -6.03
Bud Site -4.78 3.54 -4.87 0.23 -6.38 -3.31 -7.76 -6.14 -3.92 -1.84 -4.84 2.37 -7.76 -3.67 -8.64
Cell Periphery -3.41 0.63 -3.35 -1.21 -3.51 4.66 -11.67 -2.71 -5.81 10.19 2.73 -4.26 -4.08 -5.07 -1.90
Cytoplasm -2.23 5.20 27.15 20.19 24.47 -2.32 21.24 29.53 30.06 -7.10 -6.46 15.84 39.48 40.25 22.51
Cytoplasmic Foci 0.94 32.02 27.97 11.47 -9.65 2.58 15.76 20.06 15.36 2.54 5.55 38.66 34.19 19.10 -10.29
Eisosomes 4.76 14.27 -6.51 -10.11 -20.03 1.06 -11.36 -13.37 -12.75 6.35 6.21 9.15 -12.12 -17.14 -21.29
Endoplasmic Reticulum 2.01 5.27 0.64 -1.70 -6.43 3.20 -3.90 0.90 -6.34 6.92 4.94 4.34 1.13 -6.51 -6.14
Endosome 1.45 13.47 1.92 -0.06 -7.20 -0.37 -5.92 -2.17 -1.61 3.66 1.89 12.18 0.02 -1.56 -7.80
Golgi 3.28 6.31 0.39 -1.64 -3.29 -0.57 3.72 -1.21 -0.86 -2.66 0.66 7.61 -0.69 -1.08 -4.25
Lipid Particles 4.00 4.83 2.18 -3.74 -6.87 1.18 -6.95 -1.28 -1.68 3.40 3.73 2.96 0.06 -2.70 -1.76
Mitochondria 2.84 2.44 0.40 -3.03 -2.51 -1.63 2.63 -3.19 -2.05 -3.88 1.77 2.51 -0.64 -2.93 -3.92
Mitotic Spindle -0.05 -6.54 -9.02 -8.95 -6.24 -0.86 -2.14 -8.31 -7.95 -7.60 -0.66 -7.28 -12.25 -11.80 -8.20
None -0.10 -10.23 -4.44 -4.18 0.86 -1.62 4.63 -2.77 -1.02 -4.86 -2.44 -8.14 -5.16 -1.92 0.29
Nuclear Periphery 2.27 -11.48 -15.01 -15.91 -7.39 1.60 -15.24 -22.41 -23.02 0.82 3.66 -14.85 -23.32 -24.78 -7.88
Nuclear Periphery Foci -3.98 -3.28 -2.33 2.22 0.30 4.14 -9.78 -0.79 -6.21 9.65 1.82 -6.74 -2.27 -4.20 4.65
Nucleolus 1.23 1.76 -2.44 -3.71 -5.49 -0.96 -1.92 -4.24 -3.45 -1.98 0.80 1.19 -4.49 -5.29 -6.24
Nucleus 4.41 -35.65 -20.71 -21.37 9.08 3.81 -8.56 -19.20 -21.50 -1.23 6.94 -38.94 -27.99 -31.55 12.39
Peroxisomes -1.41 7.51 -1.04 1.00 -3.21 -2.18 1.82 -1.23 0.04 -1.77 -2.32 8.17 -1.60 0.61 -3.89
Vacuole 2.09 -1.41 -29.63 -27.65 -28.43 0.22 -19.22 -29.63 -29.59 8.00 3.17 -11.80 -41.76 -42.06 -27.44
Vacuole Periphery 4.79 9.03 -1.84 -2.88 -3.48 1.22 4.13 -2.39 -2.67 -3.11 2.57 10.01 -3.02 -3.66 -4.73
Standard name
Human Ortholog
Description Quinolinate phosphoribosyl transferase; required for the de novo biosynthesis of NAD from tryptophan via kynurenine; expression regulated by Hst1p
Localization
Cell Percentages cytoplasm (100%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

Bna6

Bna6


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Bna6-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available