Standard name
Human Ortholog
Description Delta subunit of the coatomer complex (COPI); COPI coats Golgi-derived transport vesicles; involved in retrograde transport between Golgi and ER

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0.16 0.2 0.11 0.1 0.09 0.08 0.07 0.08 0 0 0.07 0.08 0.2 0.16 0.12 0.15 0.16 0 0 0.05 0 0.06 0.05
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0.13 0 0.06 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endoplasmic Reticulum 0.05 0 0 0 0 0 0 0 0 0 0 0 0.07 0.06 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0.85 0.5 0.68 0.64 0.65 0.24 0.31 0.24 0.14 0.16 0.18 0.13 0.94 0.91 0.87 0.9 0.86 0.9 0.65 0.9 0.88 0.83 0.88
Mitochondria 0.49 0.7 0.72 0.74 0.65 0.87 0.82 0.83 0.87 0.9 0.84 0.88 0.25 0.16 0 0.08 0.05 0 0 0 0 0.05 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0.05 0 0.06 0.08 0.05 0.09 0.1 0 0 0 0 0.05 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.11 0.13 0.06 0 0.1 0.06 0.05 0 0 0.05 0 0 0.13 0.18 0.3 0.08 0.11 0 0.19 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 30 9 26 26 24 22 28 38 10 12 16 24 200 175 154 11 6 6 3 13 12 30 29
Bud 0 2 1 5 1 2 6 9 2 6 3 3 4 5 3 2 1 0 0 0 0 3 1
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 1 0 2 5 0 2 0 0 0 0 0 0
Cell Periphery 1 0 0 2 0 0 1 3 0 1 0 3 5 2 1 0 0 0 0 0 0 0 0
Cytoplasm 3 6 4 15 8 5 12 23 10 4 10 8 4 12 26 2 1 0 2 0 0 0 0
Endoplasmic Reticulum 10 2 6 10 7 6 7 10 9 9 8 9 72 65 48 2 1 1 1 0 0 3 2
Endosome 0 0 0 2 0 0 1 1 0 1 1 0 0 0 0 0 0 1 0 2 3 3 10
Golgi 159 23 163 168 173 68 125 110 48 46 45 40 959 995 1087 65 32 176 52 224 280 412 520
Mitochondria 92 32 173 194 173 244 334 384 294 264 204 269 260 172 54 6 2 5 2 3 12 24 17
Nucleus 0 0 0 0 0 1 1 2 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 1 0 0 0 0 1 1 5 3 2 5 0 0 0 1 0 0 0 0 0 0 1
Nucleolus 2 0 3 4 8 14 17 28 26 15 22 31 3 10 12 3 2 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 1 1 0 0 0 0 0 0 0 0 0 0 0 1 0 1 2
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 20 6 15 7 26 17 22 18 7 16 6 6 137 200 368 6 4 3 15 2 5 12 3
Unique Cell Count 188 46 241 262 265 282 406 464 339 294 244 305 1024 1096 1243 72 37 197 81 250 319 496 594
Labelled Cell Count 317 81 391 433 420 380 556 630 416 377 319 398 1644 1637 1753 98 49 197 81 250 319 496 594


Early Golgi, Golgi

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 25.9 20.7 24.8 24.1 23.6 21.7 21.1 20.0 19.0 19.5 18.1 18.9 25.9 24.8 27.7 26.8 31.9 28.0 27.4 27.8
Std Deviation (1e-4) 4.3 4.8 4.7 5.4 5.0 5.7 4.6 4.8 4.8 4.6 4.4 4.8 4.2 4.7 5.6 6.5 6.6 4.5 5.2 4.9
Intensity Change (Log2) -0.04 -0.07 -0.19 -0.23 -0.31 -0.38 -0.35 -0.45 -0.39 0.07 0.0 0.16 0.11 0.37 0.18 0.15 0.17


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches -0.3 -0.7 -1.2 -1.7 -1.1 -3.9 -3.0 -1.7 -1.2 3.2 2.0 0.7 1.0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 2.4 1.0 0 1.0 2.2 1.0 0 1.6 0.8 0 0 0 0 0
Endoplasmic Reticulum 0.8 0.1 -0.3 0 -0.3 0.1 0.4 0.5 0.3 2.6 2.2 1.0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi -0.8 -0.6 -10.0 -9.1 -11.4 -13.2 -12.3 -10.9 -13.1 11.5 9.6 7.7 3.8 2.3
Mitochondria 0.6 -1.6 4.2 3.1 3.4 4.5 5.4 3.1 4.8 -13.7 -18.0 -26.6 -9.5 -7.8
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 1.4 2.4 2.1 2.9 3.5 2.5 3.9 4.3 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole -1.9 1.5 -0.1 -0.4 -1.4 -2.6 -0.4 -2.0 -2.6 3.1 4.6 7.6 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 22.8511 29.0198 26.6021 26.2618 25.0298 26.8988 21.236 28.1786 25.45 25.303 26.0749 24.8716 22.8678 28.1007 27.003 24.5892 24.3642 24.5614
Actin 0.0218 0.0056 0.0106 0.0003 0.0031 0.0005 0.0508 0.0067 0.0126 0.0197 0.0001 0.0079 0.0692 0.0149 0.0065 0.0568 0.0028 0.0047
Bud 0.0008 0.0001 0.0007 0.0001 0.0001 0 0.0007 0.0001 0.0001 0.0001 0 0.0001 0.0003 0.0007 0.0008 0.0001 0.0001 0.0001
Bud Neck 0.0005 0.0001 0.0002 0 0.0002 0 0.0004 0.0001 0.0001 0 0 0.0001 0.0017 0.0003 0.0003 0.0002 0.0002 0.0001
Bud Periphery 0.0011 0.0001 0.0015 0 0.0001 0 0.001 0.0001 0.0001 0.0001 0 0.0001 0.0004 0.0004 0.0006 0.0001 0.0001 0.0001
Bud Site 0.0018 0.0002 0.0004 0 0.0006 0 0.0017 0.0001 0.0001 0 0 0.0001 0.0009 0.0014 0.0023 0.0002 0.0001 0.0001
Cell Periphery 0.0004 0.0002 0.0003 0 0.0002 0 0.0006 0.0001 0.0001 0 0 0.0001 0.0002 0.0002 0.0001 0.0002 0 0.0001
Cytoplasm 0.0097 0.0027 0.0028 0.0016 0.0042 0.0006 0.0028 0.0004 0.0004 0.0001 0.0001 0.0002 0.0011 0.0036 0.0013 0.0019 0.0044 0.0006
Cytoplasmic Foci 0.0162 0.021 0.0136 0.0102 0.0199 0.0074 0.0143 0.0092 0.0085 0.0052 0.0041 0.0107 0.0153 0.013 0.011 0.0346 0.0124 0.0079
Eisosomes 0.0003 0.0001 0.0001 0 0.0001 0 0.0009 0.0001 0.0001 0.0002 0 0.0001 0.0004 0.0001 0.0001 0.0002 0 0
Endoplasmic Reticulum 0.0035 0.0008 0.002 0.0003 0.0031 0.0004 0.0103 0.0003 0.0005 0.0003 0.0001 0.0006 0.0015 0.005 0.0106 0.0009 0.0012 0.0202
Endosome 0.1178 0.151 0.1429 0.1887 0.1522 0.072 0.0811 0.0818 0.0633 0.0877 0.0326 0.0415 0.0628 0.1186 0.0783 0.1154 0.0974 0.0488
Golgi 0.6772 0.7586 0.7587 0.7323 0.7385 0.8987 0.702 0.8446 0.8487 0.8082 0.9619 0.8346 0.7326 0.78 0.8095 0.6746 0.7954 0.8608
Lipid Particles 0.0149 0.0032 0.007 0.0007 0.0104 0.0006 0.0161 0.0006 0.0026 0.0005 0.0001 0.0051 0.0159 0.0087 0.0061 0.0237 0.0018 0.0106
Mitochondria 0.0704 0.0327 0.0268 0.0019 0.0131 0.0164 0.0697 0.051 0.0445 0.069 0.0006 0.0757 0.0654 0.0337 0.0553 0.0273 0.0228 0.0307
None 0.0057 0 0.0006 0 0.0033 0 0.003 0 0 0 0 0.0002 0.0017 0.0001 0.0001 0.0106 0.0024 0.0002
Nuclear Periphery 0.0051 0.0003 0.0013 0.0002 0.012 0 0.0081 0 0.0001 0.0001 0 0.0014 0.0064 0.0006 0.0003 0.0018 0.0205 0.0004
Nucleolus 0.0018 0 0.0004 0 0.0017 0 0.0003 0 0 0 0 0 0.0015 0.0001 0.0001 0.0008 0.0004 0.0001
Nucleus 0.0036 0.0001 0.0005 0 0.0045 0 0.0012 0 0 0 0 0 0.0013 0.0001 0.0001 0.0004 0.0042 0.0001
Peroxisomes 0.009 0.0002 0.0012 0 0.001 0.0001 0.0062 0.0002 0.0006 0.0001 0 0.0099 0.0037 0.0009 0.0031 0.0027 0.0006 0.0069
Punctate Nuclear 0.0058 0 0.0004 0 0.0107 0 0.0018 0 0 0 0 0.0005 0.0103 0 0.0023 0.0023 0.0274 0.0008
Vacuole 0.0098 0.0076 0.0104 0.0233 0.0057 0.0005 0.0064 0.0007 0.0031 0.0009 0 0.0007 0.0018 0.0058 0.0027 0.01 0.0011 0.0016
Vacuole Periphery 0.0227 0.0154 0.0177 0.0402 0.0154 0.0025 0.0206 0.0039 0.0144 0.0077 0.0004 0.0107 0.0056 0.0118 0.0085 0.0353 0.0044 0.0051

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 112.2904 104.5883 100.8026 104.2055 101.5639 112.2282 108.3321 95.78 103.954 115.5018
Translational Efficiency 1.0477 1.0746 0.9305 0.9756 1.0969 1.0685 1.0333 1.1724 1.0883 1.0815

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
82 196 360 1460 1456 1759 2467 1125 1538 1955 2827 2585

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 2276.05 1978.48 2984.44 2584.88 1752.31 1887.17 2630.99 2196.61 1780.23 1896.32 2676.00 2415.90
Standard Deviation 385.03 387.17 403.43 558.59 302.49 335.05 460.59 660.29 329.20 341.74 468.76 634.85
Intensity Change Log 2 -0.202140 0.390928 0.183565 0.106966 0.586348 0.326021 -0.059482 0.479204 0.247268

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.005543 0.006699 0.021925 0.002435 0.001958 0.002468 0.002805 0.002300 0.002149 0.002892 0.005240 0.002376
Bud Neck 0.005229 0.000753 0.007017 0.001073 0.000252 0.000468 0.000215 0.001932 0.000517 0.000497 0.001081 0.001447
Bud Site 0.007682 0.000824 0.002251 0.004224 0.000574 0.001047 0.000546 0.007860 0.000953 0.001024 0.000763 0.005806
Cell Periphery 0.001823 0.000448 0.000929 0.000612 0.000221 0.000159 0.000880 0.000758 0.000306 0.000188 0.000886 0.000676
Cytoplasm 0.000537 0.000079 0.000071 0.001286 0.000595 0.000490 0.000084 0.006483 0.000592 0.000449 0.000083 0.003548
Cytoplasmic Foci 0.027122 0.010697 0.007809 0.015464 0.017886 0.033802 0.011048 0.053864 0.018378 0.031486 0.010635 0.032176
Eisosomes 0.000091 0.000311 0.000605 0.000045 0.000066 0.000055 0.000260 0.000051 0.000068 0.000080 0.000304 0.000047
Endoplasmic Reticulum 0.007962 0.005669 0.003767 0.001516 0.002435 0.001899 0.005385 0.001856 0.002729 0.002277 0.005179 0.001664
Endosome 0.047494 0.028419 0.010401 0.033712 0.011676 0.022715 0.016959 0.085126 0.013585 0.023287 0.016124 0.056088
Golgi 0.670248* 0.872745* 0.718730* 0.722964* 0.901487* 0.831616* 0.875556* 0.659180* 0.889158* 0.835740* 0.855585* 0.695205*
Lipid Particles 0.041412 0.028136 0.051678 0.024585 0.008456 0.018347 0.022075 0.021671 0.010213 0.019328 0.025845 0.023317
Mitochondria 0.005547 0.011359 0.011275 0.011017 0.019846 0.018745 0.009187 0.006017 0.019084 0.018005 0.009453 0.008841
Mitotic Spindle 0.014479 0.000087 0.000939 0.005662 0.000447 0.001555 0.000258 0.013561 0.001195 0.001407 0.000345 0.009100
None 0.000087 0.000365 0.000589 0.000168 0.000238 0.000227 0.000210 0.000910 0.000229 0.000240 0.000258 0.000491
Nuclear Periphery 0.002173 0.000235 0.004200 0.001433 0.000178 0.000968 0.000376 0.001194 0.000285 0.000894 0.000863 0.001329
Nuclear Periphery Foci 0.008079 0.000367 0.002928 0.003732 0.000485 0.002128 0.000507 0.006701 0.000890 0.001951 0.000816 0.005024
Nucleolus 0.000085 0.000014 0.000121 0.000080 0.000013 0.000192 0.000013 0.000296 0.000017 0.000174 0.000027 0.000174
Nucleus 0.000265 0.000020 0.000065 0.000073 0.000068 0.000060 0.000015 0.000843 0.000079 0.000056 0.000021 0.000408
Peroxisomes 0.001056 0.000054 0.000104 0.000107 0.000080 0.000295 0.000082 0.000941 0.000132 0.000270 0.000085 0.000470
Vacuole 0.102614 0.008452 0.045327 0.086269 0.005847 0.018117 0.008472 0.077400 0.011006 0.017148 0.013165 0.082409
Vacuole Periphery 0.050474 0.024268 0.109269* 0.083544 0.027193 0.044648 0.045066 0.051056 0.028434 0.042605 0.053242 0.069405

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -0.50 -3.29 1.59 2.55 4.15 -0.83 -1.57 -0.43 0.14 0.54 -1.26 -3.95 -0.42 0.69 3.26
Bud Neck 3.39 -0.96 3.11 -1.57 5.27 -2.12 0.52 -4.80 -4.19 -4.99 0.25 -3.07 -4.68 -5.18 -1.76
Bud Site 3.56 2.76 1.57 -4.79 -2.57 -1.56 0.22 -7.82 -7.14 -7.92 -0.23 1.01 -8.34 -7.95 -8.96
Cell Periphery 3.73 2.36 3.26 -2.08 2.68 2.46 -9.52 -4.85 -5.43 0.87 3.66 -8.83 -5.91 -8.34 2.42
Cytoplasm 3.58 3.68 -2.15 -3.51 -3.54 0.29 1.18 -4.08 -4.33 -4.61 0.39 1.24 -3.97 -4.83 -5.44
Cytoplasmic Foci 3.14 3.66 2.22 -3.78 -5.72 -10.25 7.29 -11.44 -6.16 -13.93 -8.97 8.34 -8.36 -0.57 -14.38
Eisosomes -3.81 -3.36 2.06 4.84 3.69 1.45 -7.34 1.23 0.21 7.43 -1.38 -7.83 2.38 3.10 8.37
Endoplasmic Reticulum 0.79 1.65 2.56 3.02 5.92 1.59 -6.46 1.26 0.01 6.63 1.24 -5.66 3.29 1.90 9.18
Endosome 2.18 4.60 1.67 -1.38 -12.64 -8.89 -5.67 -17.72 -14.69 -16.36 -7.60 -2.61 -19.39 -14.15 -18.45
Golgi -5.49 -1.32 -1.47 10.87 -0.15 10.44 4.71 22.63 15.65 20.81* 7.96 5.61 25.69 18.53 22.89*
Lipid Particles 1.08 -0.79 1.52 0.48 4.03 -4.54 -6.64 -5.18 -1.22 0.07 -4.17 -7.41 -6.49 -1.79 1.00
Mitochondria -1.20 -1.58 -1.53 0.08 0.10 0.41 4.58 5.77 6.23 2.08 0.42 4.39 4.41 4.67 0.41
Mitotic Spindle 2.33 2.19 1.35 -4.38 -3.70 -1.99 0.76 -6.04 -5.42 -6.13 -0.31 2.07 -6.37 -6.10 -7.30
None -3.46 -9.10 -2.68 2.32 7.03 0.28 1.16 -1.92 -1.94 -2.00 -0.29 -1.21 -1.71 -1.63 -1.55
Nuclear Periphery 1.97 -1.72 0.73 -5.16 3.97 -4.98 -3.02 -4.48 -0.86 -3.52 -3.98 -4.92 -6.08 -2.07 -2.45
Nuclear Periphery Foci 3.41 2.28 1.91 -6.62 -1.28 -3.64 0.22 -5.83 -4.21 -5.96 -2.34 0.59 -6.95 -4.96 -7.73
Nucleolus 2.05 -0.91 0.07 -3.28 1.37 -1.26 0.35 -3.17 -0.76 -3.19 -1.23 -1.51 -3.81 -0.09 -3.64
Nucleus 1.61 1.31 1.21 -2.96 -0.66 0.49 1.11 -2.10 -2.24 -2.36 0.69 1.20 -1.85 -2.31 -2.54
Peroxisomes 1.68 1.59 1.58 -2.02 -0.12 -1.77 -0.09 -4.10 -2.70 -4.09 -1.22 1.12 -3.40 -1.44 -4.11
Vacuole 5.26 3.16 0.93 -19.62 -7.60 -8.99 -2.99 -19.32 -15.46 -18.64 -3.57 -1.39 -24.75 -23.23 -25.61
Vacuole Periphery 2.20 -4.41 -2.88 -11.22 3.14 -7.11 -7.97 -7.60 -1.87 -1.82 -5.98 -10.51 -15.19 -9.38 -5.64