Standard name
Description Delta subunit of the coatomer complex (COPI); COPI coats Golgi-derived transport vesicles; involved in retrograde transport between Golgi and ER
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0.16 0.2 0.11 0.1 0.09 0.08 0.07 0.08 0 0 0.07 0.08 0.2 0.16 0.12 – 0.15 0.16 0 0 0.05 0 0.06 0.05
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Cytoplasm 0 0.13 0 0.06 0 0 0 0.05 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Endoplasmic Reticulum 0.05 0 0 0 0 0 0 0 0 0 0 0 0.07 0.06 0 – 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Golgi 0.85 0.5 0.68 0.64 0.65 0.24 0.31 0.24 0.14 0.16 0.18 0.13 0.94 0.91 0.87 – 0.9 0.86 0.9 0.65 0.9 0.88 0.83 0.88
Mitochondria 0.49 0.7 0.72 0.74 0.65 0.87 0.82 0.83 0.87 0.9 0.84 0.88 0.25 0.16 0 – 0.08 0.05 0 0 0 0 0.05 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0.05 0 0.06 0.08 0.05 0.09 0.1 0 0 0 – 0 0.05 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.11 0.13 0.06 0 0.1 0.06 0.05 0 0 0.05 0 0 0.13 0.18 0.3 – 0.08 0.11 0 0.19 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 30 9 26 26 24 22 28 38 10 12 16 24 200 175 154 – 11 6 6 3 13 12 30 29
Bud 0 2 1 5 1 2 6 9 2 6 3 3 4 5 3 – 2 1 0 0 0 0 3 1
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 1 0 2 5 0 2 0 0 0 0 – 0 0 – – – – – –
Cell Periphery 1 0 0 2 0 0 1 3 0 1 0 3 5 2 1 – 0 0 0 0 0 0 0 0
Cytoplasm 3 6 4 15 8 5 12 23 10 4 10 8 4 12 26 – 2 1 0 2 0 0 0 0
Endoplasmic Reticulum 10 2 6 10 7 6 7 10 9 9 8 9 72 65 48 – 2 1 1 1 0 0 3 2
Endosome 0 0 0 2 0 0 1 1 0 1 1 0 0 0 0 – 0 0 1 0 2 3 3 10
Golgi 159 23 163 168 173 68 125 110 48 46 45 40 959 995 1087 – 65 32 176 52 224 280 412 520
Mitochondria 92 32 173 194 173 244 334 384 294 264 204 269 260 172 54 – 6 2 5 2 3 12 24 17
Nucleus 0 0 0 0 0 1 1 2 0 0 0 0 0 1 0 – 0 0 0 0 0 0 0 0
Nuclear Periphery 0 1 0 0 0 0 1 1 5 3 2 5 0 0 0 – 1 0 0 0 0 0 0 1
Nucleolus 2 0 3 4 8 14 17 28 26 15 22 31 3 10 12 – 3 2 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 1 1 0 0 0 0 0 0 0 – 0 0 0 0 1 0 1 2
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Vac/Vac Membrane 20 6 15 7 26 17 22 18 7 16 6 6 137 200 368 – 6 4 3 15 2 5 12 3
Unique Cell Count 188 46 241 262 265 282 406 464 339 294 244 305 1024 1096 1243 72 37 197 81 250 319 496 594
Labelled Cell Count 317 81 391 433 420 380 556 630 416 377 319 398 1644 1637 1753 98 49 197 81 250 319 496 594


Early Golgi, Golgi

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 25.9 20.7 24.8 24.1 23.6 21.7 21.1 20.0 19.0 19.5 18.1 18.9 25.9 24.8 27.7 – 26.8 31.9 28.0 27.4 27.8
Std Deviation (1e-4) 4.3 4.8 4.7 5.4 5.0 5.7 4.6 4.8 4.8 4.6 4.4 4.8 4.2 4.7 5.6 – 6.5 6.6 4.5 5.2 4.9
Intensity Change (Log2) – – – -0.04 -0.07 -0.19 -0.23 -0.31 -0.38 -0.35 -0.45 -0.39 0.07 0.0 0.16 – 0.11 0.37 0.18 0.15 0.17


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches -0.3 -0.7 -1.2 -1.7 -1.1 -3.9 -3.0 -1.7 -1.2 3.2 2.0 0.7 – 1.0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0
Cytoplasm 2.4 1.0 0 1.0 2.2 1.0 0 1.6 0.8 0 0 0 – 0 0
Endoplasmic Reticulum 0.8 0.1 -0.3 0 -0.3 0.1 0.4 0.5 0.3 2.6 2.2 1.0 – 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0
Golgi -0.8 -0.6 -10.0 -9.1 -11.4 -13.2 -12.3 -10.9 -13.1 11.5 9.6 7.7 – 3.8 2.3
Mitochondria 0.6 -1.6 4.2 3.1 3.4 4.5 5.4 3.1 4.8 -13.7 -18.0 -26.6 – -9.5 -7.8
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0
Nucleolus 0 1.4 2.4 2.1 2.9 3.5 2.5 3.9 4.3 0 0 0 – 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0
Vacuole -1.9 1.5 -0.1 -0.4 -1.4 -2.6 -0.4 -2.0 -2.6 3.1 4.6 7.6 – 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 22.8511 29.0198 26.6021 26.2618 25.0298 26.8988 21.236 28.1786 25.45 25.303 26.0749 24.8716 22.8678 28.1007 27.003 24.5892 24.3642 24.5614
Actin 0.0218 0.0056 0.0106 0.0003 0.0031 0.0005 0.0508 0.0067 0.0126 0.0197 0.0001 0.0079 0.0692 0.0149 0.0065 0.0568 0.0028 0.0047
Bud 0.0008 0.0001 0.0007 0.0001 0.0001 0 0.0007 0.0001 0.0001 0.0001 0 0.0001 0.0003 0.0007 0.0008 0.0001 0.0001 0.0001
Bud Neck 0.0005 0.0001 0.0002 0 0.0002 0 0.0004 0.0001 0.0001 0 0 0.0001 0.0017 0.0003 0.0003 0.0002 0.0002 0.0001
Bud Periphery 0.0011 0.0001 0.0015 0 0.0001 0 0.001 0.0001 0.0001 0.0001 0 0.0001 0.0004 0.0004 0.0006 0.0001 0.0001 0.0001
Bud Site 0.0018 0.0002 0.0004 0 0.0006 0 0.0017 0.0001 0.0001 0 0 0.0001 0.0009 0.0014 0.0023 0.0002 0.0001 0.0001
Cell Periphery 0.0004 0.0002 0.0003 0 0.0002 0 0.0006 0.0001 0.0001 0 0 0.0001 0.0002 0.0002 0.0001 0.0002 0 0.0001
Cytoplasm 0.0097 0.0027 0.0028 0.0016 0.0042 0.0006 0.0028 0.0004 0.0004 0.0001 0.0001 0.0002 0.0011 0.0036 0.0013 0.0019 0.0044 0.0006
Cytoplasmic Foci 0.0162 0.021 0.0136 0.0102 0.0199 0.0074 0.0143 0.0092 0.0085 0.0052 0.0041 0.0107 0.0153 0.013 0.011 0.0346 0.0124 0.0079
Eisosomes 0.0003 0.0001 0.0001 0 0.0001 0 0.0009 0.0001 0.0001 0.0002 0 0.0001 0.0004 0.0001 0.0001 0.0002 0 0
Endoplasmic Reticulum 0.0035 0.0008 0.002 0.0003 0.0031 0.0004 0.0103 0.0003 0.0005 0.0003 0.0001 0.0006 0.0015 0.005 0.0106 0.0009 0.0012 0.0202
Endosome 0.1178 0.151 0.1429 0.1887 0.1522 0.072 0.0811 0.0818 0.0633 0.0877 0.0326 0.0415 0.0628 0.1186 0.0783 0.1154 0.0974 0.0488
Golgi 0.6772 0.7586 0.7587 0.7323 0.7385 0.8987 0.702 0.8446 0.8487 0.8082 0.9619 0.8346 0.7326 0.78 0.8095 0.6746 0.7954 0.8608
Lipid Particles 0.0149 0.0032 0.007 0.0007 0.0104 0.0006 0.0161 0.0006 0.0026 0.0005 0.0001 0.0051 0.0159 0.0087 0.0061 0.0237 0.0018 0.0106
Mitochondria 0.0704 0.0327 0.0268 0.0019 0.0131 0.0164 0.0697 0.051 0.0445 0.069 0.0006 0.0757 0.0654 0.0337 0.0553 0.0273 0.0228 0.0307
None 0.0057 0 0.0006 0 0.0033 0 0.003 0 0 0 0 0.0002 0.0017 0.0001 0.0001 0.0106 0.0024 0.0002
Nuclear Periphery 0.0051 0.0003 0.0013 0.0002 0.012 0 0.0081 0 0.0001 0.0001 0 0.0014 0.0064 0.0006 0.0003 0.0018 0.0205 0.0004
Nucleolus 0.0018 0 0.0004 0 0.0017 0 0.0003 0 0 0 0 0 0.0015 0.0001 0.0001 0.0008 0.0004 0.0001
Nucleus 0.0036 0.0001 0.0005 0 0.0045 0 0.0012 0 0 0 0 0 0.0013 0.0001 0.0001 0.0004 0.0042 0.0001
Peroxisomes 0.009 0.0002 0.0012 0 0.001 0.0001 0.0062 0.0002 0.0006 0.0001 0 0.0099 0.0037 0.0009 0.0031 0.0027 0.0006 0.0069
Punctate Nuclear 0.0058 0 0.0004 0 0.0107 0 0.0018 0 0 0 0 0.0005 0.0103 0 0.0023 0.0023 0.0274 0.0008
Vacuole 0.0098 0.0076 0.0104 0.0233 0.0057 0.0005 0.0064 0.0007 0.0031 0.0009 0 0.0007 0.0018 0.0058 0.0027 0.01 0.0011 0.0016
Vacuole Periphery 0.0227 0.0154 0.0177 0.0402 0.0154 0.0025 0.0206 0.0039 0.0144 0.0077 0.0004 0.0107 0.0056 0.0118 0.0085 0.0353 0.0044 0.0051

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 112.2904 104.5883 100.8026 104.2055 101.5639 112.2282 108.3321 95.78 103.954 115.5018
Translational Efficiency 1.0477 1.0746 0.9305 0.9756 1.0969 1.0685 1.0333 1.1724 1.0883 1.0815

Hit Data

Dataset Hit
Protein Abundance ✔
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘