Standard name
Human Ortholog
Description High-affinity zinc transporter of the plasma membrane; responsible for the majority of zinc uptake; transcription is induced under low-zinc conditions by the Zap1p transcription factor
DOWNLOAD

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 – 0 – 0 0 0 0 – 0 0 0 0.12 0.1 0.08 0 0 0 0 0 0
Bud 0 0 0 0 – 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 – 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 – 0 – 0 0 0 0 – 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 – 0 – 0 0 0 0 – 0 0 0 0.08 0.14 0.14 0 0 0 0 0 0
Cytoplasm 0.96 0.98 0.97 0.97 – 0.87 – 0.77 0.73 0.76 0.71 – 0.96 0.98 0.98 0.65 0.58 0.58 0.85 0.8 0.78 0.71 0.68 0.69
Endoplasmic Reticulum 0 0 0 0 – 0 – 0 0 0 0 – 0 0 0 0 0 0.05 0 0 0 0 0 0
Endosome 0 0 0 0 – 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 – 0 – 0 0 0 0 – 0 0 0 0 0.05 0 0 0 0 0 0 0
Mitochondria 0 0 0.06 0.05 – 0.31 – 0.36 0.46 0.35 0.51 – 0 0 0 0.07 0.07 0.06 0.05 0.05 0.09 0.11 0.11 0.13
Nucleus 0 0 0 0 – 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 – 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 – 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 – 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 – 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 – 0 – 0 0 0 0 – 0 0 0 0.09 0.11 0.14 0 0 0 0.05 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 – 0 – 0 1 0 0 – 0 0 0 22 11 13 1 3 7 2 3 4
Bud 0 0 1 1 – 15 – 15 25 16 29 – 0 0 0 2 2 1 1 7 4 4 10 13
Bud Neck 1 4 3 2 – 15 – 4 1 12 4 – 0 2 1 0 0 0 0 2 1 3 2 2
Bud Site 0 0 0 0 – 0 – 1 5 10 5 – 0 0 0 2 0 0 – – – – – –
Cell Periphery 1 0 0 3 – 0 – 2 2 2 3 – 4 2 0 15 15 22 0 3 3 2 1 2
Cytoplasm 164 375 87 433 – 600 – 636 582 660 473 – 333 419 356 118 61 93 146 315 285 200 218 218
Endoplasmic Reticulum 3 2 2 1 – 0 – 0 0 1 0 – 12 5 4 2 3 8 2 4 3 7 10 7
Endosome 0 1 0 0 – 0 – 1 2 0 0 – 1 1 1 6 3 4 1 3 4 0 4 2
Golgi 0 1 0 0 – 0 – 0 0 0 0 – 1 0 1 3 5 7 0 0 1 2 1 2
Mitochondria 7 7 5 23 – 215 – 296 371 305 336 – 0 0 0 13 7 9 8 21 32 32 35 40
Nucleus 1 0 0 0 – 2 – 8 3 1 1 – 2 0 1 3 1 1 0 0 0 0 0 2
Nuclear Periphery 0 0 0 0 – 5 – 1 0 5 1 – 0 1 0 0 0 1 0 0 0 0 1 0
Nucleolus 0 0 0 0 – 0 – 1 3 2 1 – 1 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 – 0 – 1 1 1 2 – 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 4 – 5 – 17 18 31 15 – 0 0 1 4 1 3 1 6 5 1 10 3
Vac/Vac Membrane 0 3 0 2 – 1 – 6 11 9 2 – 3 2 2 17 12 23 2 11 2 12 13 12
Unique Cell Count 171 383 90 445 690 825 800 864 663 347 427 364 182 105 161 172 393 366 280 321 318
Labelled Cell Count 177 393 98 469 858 989 1025 1055 872 357 432 367 207 121 185 172 393 366 280 321 318


Endoplasmic Reticulum

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.5 3.9 2.9 3.1 – 2.9 – 2.8 2.5 2.4 2.4 – 5.2 4.7 5.1 10.1 11.2 12.0 5.3 5.3 5.4
Std Deviation (1e-4) 0.9 0.9 0.4 1.7 – 1.2 – 1.3 1.0 0.8 0.9 – 1.7 1.1 1.6 4.9 5.6 6.5 1.7 1.9 2.1
Intensity Change (Log2) – – – 0.12 – 0.01 – -0.07 -0.22 -0.26 -0.3 – 0.84 0.68 0.81 1.8 1.95 2.05 0.86 0.87 0.9


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – 0 0 0
Bud Neck – – – – – – – – – – – – 0 0 0
Bud Site – – – – – – – – – – – – 0 0 0
Cell Periphery – – – – – – – – – – – – 0 0 0
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – 0 0 0
Endosome – – – – – – – – – – – – 0 0 0
Golgi – – – – – – – – – – – – 0 0 0
Mitochondria – – – – – – – – – – – – 0 0 0
Nuclear Periphery – – – – – – – – – – – – 0 0 0
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – 0 0 0
Peroxisomes – – – – – – – – – – – – 0 0 0
SpindlePole – – – – – – – – – – – – 0 0 0
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – 0 0 0
Cytoplasm – – – – – – – – – – – – 0 0 0
Nucleus – – – – – – – – – – – – 0 0 0
Vacuole – – – – – – – – – – – – 3.0 3.3 3.8

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 1.3161 1.303 2.1086 1.2099 0.9701 0.8931 1.1564 1.2913 1.5474 1.6938 2.0263 1.228 -1.0208 -0.885 -0.9641 -1.2126 -0.9086 -0.9704
Actin 0.0135 0.0033 0.0077 0.0034 0.0055 0.0074 0.0379 0.0008 0.0011 0.0004 0.0143 0.0085 0.0061 0.0004 0.012 0.0005 0.015 0.0015
Bud 0.0128 0.0222 0.0166 0.0234 0.0063 0.0096 0.0006 0.0007 0.0017 0.0003 0.0005 0.001 0.0015 0.0027 0.0034 0 0.0008 0.0003
Bud Neck 0.0072 0.0008 0.0046 0.002 0.0017 0.0037 0.0031 0.0002 0.0002 0.0008 0.0006 0.0007 0.0007 0.0002 0.0005 0.0001 0.0058 0.0009
Bud Periphery 0.0161 0.0237 0.0231 0.038 0.0052 0.012 0.0016 0.0024 0.0048 0.0003 0.0023 0.0034 0.0058 0.0035 0.0074 0.0001 0.0015 0.0028
Bud Site 0.0404 0.0342 0.0084 0.0196 0.0019 0.001 0.0086 0.0013 0.001 0.0023 0.0007 0.0004 0.0024 0.006 0.0048 0.0001 0.002 0.0001
Cell Periphery 0.0039 0.0032 0.0026 0.0014 0.0006 0.0005 0.0013 0.0021 0.0012 0.0002 0.0009 0.0007 0.0013 0.001 0.0011 0.0001 0.0002 0.0003
Cytoplasm 0.0686 0.1187 0.0764 0.0688 0.1292 0.1148 0.0338 0.0455 0.0791 0.09 0.0748 0.0732 0.0393 0.0898 0.0835 0.0667 0.0333 0.1102
Cytoplasmic Foci 0.0384 0.0098 0.0172 0.0304 0.0156 0.0266 0.0134 0.0025 0.0078 0.0024 0.0341 0.0075 0.007 0.0024 0.0056 0.0048 0.0066 0.0051
Eisosomes 0.0007 0.0002 0.0005 0.0003 0.0004 0.0001 0.0011 0.0003 0.0004 0.0002 0.0001 0.0001 0.0007 0.0003 0.0004 0.0002 0.0006 0.0001
Endoplasmic Reticulum 0.1101 0.1388 0.2194 0.123 0.0706 0.1379 0.063 0.0585 0.1027 0.1699 0.1111 0.0817 0.0693 0.0368 0.0776 0.0057 0.007 0.052
Endosome 0.0424 0.0045 0.0146 0.0386 0.0287 0.0174 0.0139 0.0026 0.004 0.0014 0.0203 0.0025 0.004 0.0007 0.0068 0.003 0.0126 0.0048
Golgi 0.0125 0.0005 0.0024 0.0212 0.0123 0.0061 0.004 0.0002 0.0003 0.0001 0.0044 0.001 0.0005 0.0001 0.0021 0.0002 0.0024 0.0004
Lipid Particles 0.0231 0.0012 0.0037 0.012 0.022 0.0057 0.0141 0.009 0.0026 0.0003 0.0277 0.0033 0.0018 0.0003 0.0019 0.0007 0.0083 0.0015
Mitochondria 0.0317 0.0034 0.0172 0.0279 0.0454 0.0152 0.0037 0.0004 0.0005 0.0003 0.0017 0.0013 0.0015 0.0004 0.0016 0.0004 0.0074 0.0005
None 0.4742 0.5602 0.5241 0.4845 0.543 0.5709 0.7657 0.8475 0.7656 0.7233 0.6045 0.7951 0.8265 0.8475 0.7641 0.9124 0.8313 0.7982
Nuclear Periphery 0.0294 0.026 0.0244 0.0203 0.0432 0.0216 0.009 0.0112 0.0148 0.0036 0.0102 0.006 0.0157 0.0037 0.0137 0.0017 0.0177 0.013
Nucleolus 0.0063 0.0027 0.0012 0.0042 0.0039 0.0021 0.002 0.0003 0.0003 0.0003 0.0005 0.0001 0.0009 0.0003 0.0004 0.0001 0.0033 0.0002
Nucleus 0.0151 0.0206 0.0091 0.0218 0.0194 0.0115 0.0022 0.0028 0.0032 0.0013 0.0028 0.0021 0.004 0.0014 0.0042 0.0007 0.0123 0.0024
Peroxisomes 0.021 0.0016 0.0086 0.0317 0.0072 0.012 0.0114 0.0001 0.0004 0.0001 0.0614 0.0057 0.0005 0.0001 0.0006 0.0004 0.0063 0.0002
Punctate Nuclear 0.0078 0.0132 0.0056 0.0162 0.0219 0.0145 0.0038 0.0006 0.0018 0.0011 0.0161 0.0015 0.0057 0.0004 0.0028 0.0017 0.0226 0.0015
Vacuole 0.0195 0.0096 0.0099 0.0093 0.0101 0.0076 0.0048 0.0097 0.0041 0.0012 0.0098 0.0034 0.0034 0.0016 0.0041 0.0003 0.002 0.0031
Vacuole Periphery 0.0057 0.0017 0.0027 0.002 0.0056 0.0018 0.001 0.0013 0.0025 0.0002 0.0012 0.0007 0.0014 0.0005 0.0015 0.0001 0.0008 0.001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 38.5362 37.5015 30.4163 17.3107 8.2219 33.1521 33.2882 24.6992 20.1935 22.8218
Translational Efficiency 0.789 1.1453 0.8693 1.0859 1.434 0.8942 0.9144 1.0655 0.8231 0.6456

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1321 1288 1573 1244 1197 2165 2014 1714 2518 3453 3587 2958

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 600.58 581.68 791.26 1021.21 591.88 657.85 832.24 1034.43 596.44 629.44 814.27 1028.87
Standard Deviation 95.58 78.68 160.04 504.74 97.58 141.85 161.38 348.56 96.63 127.60 162.07 421.41
Intensity Change Log 2 — -0.046131 0.397795 0.765851 — 0.152454 0.491695 0.805459 — 0.055852 0.445167 0.785647

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000243 0.000104 0.000277 0.006080 0.000099 0.000245 0.000760 0.004475 0.000174 0.000193 0.000548 0.005150
Bud Neck 0.020296 0.023104 0.005511 0.018777 0.010800 0.043775 0.007188 0.009356 0.015782 0.036065 0.006453 0.013318
Bud Site 0.011453 0.004837 0.007837 0.085840 0.002746 0.012118 0.012159 0.055766 0.007314 0.009402 0.010264 0.068414
Cell Periphery 0.000735 0.000120 0.000414 0.006445 0.000228 0.000206 0.000522 0.006694 0.000494 0.000174 0.000475 0.006589
Cytoplasm 0.465311* 0.445428* 0.287093* 0.298288* 0.449303* 0.401440* 0.367850* 0.319094* 0.457701* 0.417848* 0.332435* 0.310344*
Cytoplasmic Foci 0.208335* 0.198290* 0.007116 0.024923 0.215767* 0.243318* 0.012707 0.024635 0.211868* 0.226522* 0.010255 0.024756
Eisosomes 0.000385 0.000137 0.000027 0.000339 0.000190 0.000194 0.000057 0.000282 0.000292 0.000173 0.000044 0.000306
Endoplasmic Reticulum 0.028722 0.001214 0.044731 0.232015* 0.015537 0.001779 0.076535 0.275958* 0.022454 0.001569 0.062588 0.257478*
Endosome 0.013149 0.018362 0.002190 0.012232 0.013998 0.016627 0.003160 0.009696 0.013553 0.017274 0.002735 0.010762
Golgi 0.002242 0.001668 0.000086 0.002514 0.001586 0.003872 0.000478 0.001997 0.001930 0.003050 0.000306 0.002214
Lipid Particles 0.019509 0.014352 0.001701 0.013540 0.016990 0.012824 0.002793 0.011844 0.018312 0.013394 0.002314 0.012557
Mitochondria 0.006491 0.007506 0.001264 0.002804 0.004463 0.013730 0.001621 0.003924 0.005527 0.011409 0.001464 0.003453
Mitotic Spindle 0.000822 0.000594 0.002004 0.021449 0.000439 0.002352 0.004999 0.007890 0.000640 0.001697 0.003685 0.013592
None 0.008988 0.018120 0.005920 0.005458 0.013690 0.004094 0.005081 0.004064 0.011223 0.009326 0.005449 0.004651
Nuclear Periphery 0.002003 0.000792 0.014629 0.006288 0.001756 0.000884 0.019128 0.002573 0.001886 0.000850 0.017155 0.004136
Nuclear Periphery Foci 0.001542 0.000567 0.008657 0.019086 0.000942 0.000419 0.008936 0.008583 0.001256 0.000474 0.008814 0.013000
Nucleolus 0.001353 0.005928 0.000539 0.000401 0.002825 0.001589 0.000467 0.000253 0.002053 0.003207 0.000499 0.000315
Nucleus 0.150595* 0.190133* 0.582730* 0.104710 0.200409* 0.174867* 0.430409* 0.136683* 0.174275* 0.180561* 0.497206* 0.123237
Peroxisomes 0.005362 0.006860 0.000573 0.001418 0.006722 0.014457 0.000871 0.001021 0.006008 0.011624 0.000740 0.001188
Vacuole 0.051568 0.060206 0.026462 0.134696 0.040502 0.049853 0.043874 0.113643 0.046308 0.053715 0.036239 0.122497
Vacuole Periphery 0.000896 0.001677 0.000240 0.002697 0.001010 0.001355 0.000403 0.001569 0.000950 0.001475 0.000331 0.002044

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin 3.37 -0.90 -6.51 -6.70 -6.46 -4.71 -2.32 -6.71 -6.47 -5.42 -0.77 -2.33 -9.31 -9.27 -8.34
Bud Neck -1.03 7.55 1.29 2.51 -7.52 -12.54 2.32 0.92 14.49 -2.05 -10.38 7.32 2.24 13.48 -6.93
Bud Site 3.40 1.75 -11.99 -14.06 -13.17 -7.50 -5.43 -14.59 -11.45 -11.37 -1.69 -1.56 -18.00 -17.84 -17.37
Cell Periphery 3.94 2.16 -6.02 -7.04 -6.66 0.45 -2.24 -8.10 -8.13 -7.70 3.77 0.76 -9.94 -10.73 -10.17
Cytoplasm 2.32 19.63 13.96 11.52 -3.34 4.72 9.82 11.33 8.08 2.42 5.77 20.05 17.94 13.44 -0.41
Cytoplasmic Foci 1.02 37.23 32.87 31.46 -10.62 -3.58 34.59 32.04 43.81 -7.45 -2.84 50.61 45.94 53.71 -12.42
Eisosomes 4.44 6.42 1.47 -4.42 -7.68 -0.21 8.16 -1.82 -1.77 -5.92 3.88 8.20 0.59 -3.90 -9.34
Endoplasmic Reticulum 6.89 -1.77 -14.74 -19.01 -13.92 4.51 -8.85 -22.19 -24.84 -16.28 8.18 -7.57 -26.16 -31.21 -21.40
Endosome -3.28 10.60 2.28 5.58 -10.08 -1.56 7.58 3.41 6.68 -6.85 -3.28 12.40 4.24 8.64 -11.49
Golgi 1.68 7.56 0.59 -0.48 -4.02 -5.07 3.75 0.05 4.51 -3.01 -3.62 7.46 0.63 3.60 -4.92
Lipid Particles 2.86 11.82 3.98 1.39 -8.90 2.53 10.38 3.91 1.93 -7.51 4.02 15.64 5.72 2.31 -11.47
Mitochondria -0.53 3.97 2.94 4.85 -4.14 -6.02 3.06 0.69 7.40 -3.17 -4.95 4.97 2.51 8.89 -4.32
Mitotic Spindle 0.29 -2.31 -7.48 -7.66 -6.64 -3.38 -4.07 -4.97 -3.48 -1.39 -2.52 -4.58 -8.88 -8.12 -5.98
None -3.33 2.38 2.62 5.38 0.80 4.64 4.38 4.73 0.52 1.48 1.41 4.99 5.43 4.79 1.66
Nuclear Periphery 2.18 -6.36 -3.67 -5.61 3.76 1.98 -8.56 -1.73 -5.09 8.23 2.81 -10.60 -3.89 -7.20 8.78
Nuclear Periphery Foci 3.10 -5.44 -8.82 -9.52 -4.51 2.34 -7.79 -10.69 -11.90 -0.10 3.92 -9.24 -12.54 -13.78 -3.72
Nucleolus -7.90 3.56 6.33 9.73 2.23 3.16 6.27 6.79 7.25 4.45 -3.58 7.34 8.72 11.83 3.93
Nucleus -5.62 -50.69 3.06 7.83 50.23 3.67 -28.71 5.75 2.72 35.62 -1.20 -54.31 5.68 7.01 58.56
Peroxisomes -2.36 7.98 7.43 9.59 -1.52 -5.65 5.86 5.87 15.29 -0.57 -7.00 9.20 8.97 17.68 -1.46
Vacuole -2.75 6.59 -18.44 -16.67 -21.54 -3.42 -4.89 -20.28 -18.85 -17.01 -3.62 0.20 -27.09 -25.28 -27.07
Vacuole Periphery -3.94 7.01 -2.07 -0.71 -3.33 -1.92 3.86 -0.88 0.19 -3.08 -4.12 6.72 -2.21 -0.49 -4.52
Standard name
Human Ortholog
Description High-affinity zinc transporter of the plasma membrane; responsible for the majority of zinc uptake; transcription is induced under low-zinc conditions by the Zap1p transcription factor
Localization
Cell Percentages —
Cell Cycle Regulation No
Subcompartmental Group N/A

Zrt1

Zrt1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Zrt1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available