Standard name
Human Ortholog
Description Sensor of mitochondrial dysfunction; regulates the subcellular location of Rtg1p and Rtg3p, transcriptional activators of the retrograde (RTG) and TOR pathways; Rtg2p is inhibited by the phosphorylated form of Mks1p
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 – 0 0 0 0 0 0 0 0 0.05 – – – 0 0 0 0 0 0 0 0 0
Bud 0 0 – 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 – 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0 0 0 0
Bud Site 0 0 – 0 0 0 0 0 0 0 0 0 – – – 0 0 0 – – – – – –
Cell Periphery 0 0 – 0 0.06 0.06 0.08 0.07 0 0.08 0.07 0 – – – 0 0.07 0.05 0 0 0 0 0 0
Cytoplasm 0.99 0.97 – 0.88 0.93 0.94 0.85 0.83 0.8 0.77 0.82 0.67 – – – 0.91 0.92 0.92 0.95 0.95 0.88 0.18 0.67 0.58
Endoplasmic Reticulum 0 0 – 0 0 0 0.07 0.11 0 0.14 0.07 0.07 – – – 0 0 0 0 0 0 0 0 0
Endosome 0 0 – 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0 0.39 0.14 0.12
Golgi 0 0 – 0 0 0 0 0 0 0 0 0.05 – – – 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 – 0 0 0 0 0 0.15 0.1 0.15 0.26 – – – 0 0 0 0 0 0 0 0 0
Nucleus 0 0 – 0.08 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 – 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0 0.05 0 0
Nucleolus 0 0 – 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 – 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 – 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 – 0 0 0 0 0 0 0 0 0 – – – 0.08 0 0 0 0 0 0 0.06 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 1 – 0 0 0 6 7 0 5 1 2 – – – 1 2 0 0 0 0 0 0 0
Bud 0 0 – 0 0 0 1 1 0 0 0 0 – – – 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 – 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0 0 0 0
Bud Site 0 0 – 0 0 0 0 0 0 0 0 0 – – – 0 0 0 – – – – – –
Cell Periphery 3 10 – 0 7 4 13 12 1 10 3 0 – – – 14 22 17 0 0 0 0 0 0
Cytoplasm 223 255 – 23 114 65 139 147 32 95 33 29 – – – 354 306 295 218 255 33 1 12 13
Endoplasmic Reticulum 2 6 – 0 3 3 12 19 0 17 3 3 – – – 8 9 12 5 6 1 0 0 0
Endosome 0 0 – 0 0 0 0 0 0 0 0 0 – – – 0 0 1 0 0 0 2 2 2
Golgi 0 0 – 0 0 0 2 1 0 0 0 2 – – – 3 3 1 0 0 0 0 0 0
Mitochondria 0 0 – 0 0 0 6 4 6 12 6 11 – – – 3 3 0 1 0 1 0 0 1
Nucleus 0 0 – 2 0 0 0 1 0 0 0 0 – – – 2 2 0 0 1 0 0 0 0
Nuclear Periphery 0 0 – 1 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 – 0 0 0 0 0 0 0 0 1 – – – 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 – 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 – 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 1 – 1 3 0 5 1 1 0 0 1 – – – 31 7 14 0 5 0 0 1 0
Unique Cell Count 225 262 26 123 69 164 177 40 124 40 43 387 331 321 229 270 38 7 18 24
Labelled Cell Count 228 273 27 127 72 184 193 40 139 46 49 416 354 340 229 270 38 7 18 24


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 12.2 15.1 – 10.5 15.6 12.8 13.3 12.3 9.4 11.2 10.1 9.4 – – – 20.7 20.3 19.3 – – –
Std Deviation (1e-4) 1.8 2.7 – 2.4 2.3 2.1 2.1 2.1 1.9 2.3 2.2 1.6 – – – 4.2 4.1 4.0 – – –
Intensity Change (Log2) – – – – – – – – – – – – – – – – – – – – –


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 7.4372 11.0378 9.3015 9.1487 7.5652 9.561 6.1814 9.6311 8.8504 8.4251 6.0034 9.031 7.4252 10.482 9.3531 7.5774 8.8297 9.4701
Actin 0.0072 0.0011 0.0079 0.0054 0.0018 0.0012 0.0247 0.0027 0.0081 0.0026 0.0323 0.0012 0.0071 0.0003 0.0051 0.0097 0.0111 0.0055
Bud 0.001 0.003 0.0031 0.001 0.0005 0.0036 0.0006 0.001 0.0005 0.0011 0.0005 0.0004 0.0007 0.0003 0.0024 0.0008 0.0005 0.0003
Bud Neck 0.0009 0.0004 0.0014 0.0007 0.0012 0.0034 0.0011 0.0002 0.0004 0.0016 0.0019 0.0009 0.0012 0.0002 0.0023 0.001 0.0009 0.0008
Bud Periphery 0.0009 0.0008 0.0023 0.0006 0.0006 0.0027 0.0007 0.0006 0.0002 0.0008 0.0008 0.0003 0.0009 0.0001 0.0047 0.0008 0.0007 0.0002
Bud Site 0.0025 0.0021 0.0061 0.0007 0.0009 0.0005 0.0011 0.0011 0.0011 0.001 0.0037 0.0001 0.0014 0.0009 0.0026 0.0039 0.0008 0.0004
Cell Periphery 0.0008 0.0004 0.0006 0.0001 0.0003 0.0005 0.0005 0.0001 0.0002 0.0003 0.0002 0.0002 0.0005 0.0001 0.0002 0.0002 0.0001 0.0001
Cytoplasm 0.7407 0.9417 0.8157 0.7957 0.6427 0.8722 0.7423 0.9438 0.8635 0.6957 0.5865 0.8379 0.8456 0.9674 0.8887 0.748 0.8541 0.883
Cytoplasmic Foci 0.0223 0.0085 0.0316 0.017 0.051 0.0173 0.0295 0.0113 0.0153 0.0254 0.0312 0.0181 0.0209 0.0066 0.0132 0.0227 0.0164 0.0116
Eisosomes 0.0002 0 0.0002 0 0.0001 0 0.0004 0 0.0002 0 0.0004 0 0.0001 0 0.0005 0.0003 0.0001 0.0001
Endoplasmic Reticulum 0.0484 0.0157 0.0118 0.0224 0.005 0.0142 0.0403 0.005 0.0165 0.0141 0.0209 0.0431 0.0365 0.0047 0.0147 0.004 0.0212 0.0345
Endosome 0.085 0.0151 0.0644 0.0999 0.1191 0.0507 0.0837 0.0224 0.0491 0.1051 0.1109 0.0545 0.0377 0.0131 0.0287 0.0831 0.0503 0.0358
Golgi 0.018 0.0025 0.0139 0.0226 0.0422 0.0054 0.013 0.0029 0.0065 0.0144 0.0222 0.0088 0.01 0.0016 0.0026 0.0087 0.0033 0.0053
Lipid Particles 0.0082 0.0001 0.0028 0.0003 0.02 0.0001 0.0061 0 0.0037 0.0143 0.0445 0.0008 0.0055 0 0.0017 0.0112 0.003 0.0028
Mitochondria 0.0228 0.0012 0.0136 0.0054 0.0866 0.0134 0.0063 0.0011 0.0049 0.0416 0.0682 0.0246 0.0042 0.0003 0.0145 0.039 0.0012 0.0083
None 0.0028 0.0007 0.001 0.0006 0.0011 0.0009 0.0074 0.0008 0.0035 0.0005 0.0032 0.0008 0.0057 0.0005 0.001 0.0011 0.0014 0.0017
Nuclear Periphery 0.0062 0.0011 0.0015 0.0032 0.001 0.0011 0.0103 0.0006 0.002 0.0033 0.0066 0.0009 0.0039 0.0004 0.0018 0.0031 0.0183 0.0032
Nucleolus 0.0015 0 0.0011 0 0.0005 0 0.0004 0 0.0001 0.0001 0.0008 0 0.0003 0 0.0001 0.0007 0.0005 0.0001
Nucleus 0.0028 0.0008 0.0025 0.0018 0.0012 0.0012 0.0028 0.0007 0.0012 0.0014 0.0021 0.0008 0.002 0.0005 0.0009 0.0012 0.0033 0.001
Peroxisomes 0.0031 0.0001 0.0024 0.0001 0.0027 0.0002 0.0037 0.0001 0.0035 0.0152 0.0293 0.0003 0.0036 0 0.0027 0.0227 0.0007 0.0007
Punctate Nuclear 0.0007 0.0001 0.0008 0.0001 0.0006 0.0001 0.0032 0.0001 0.0008 0.0005 0.0131 0.0001 0.003 0 0.0002 0.0004 0.0064 0.0006
Vacuole 0.0192 0.0038 0.0125 0.0176 0.0149 0.0084 0.0187 0.0048 0.0162 0.0416 0.0155 0.004 0.0078 0.0024 0.0093 0.0293 0.0037 0.0027
Vacuole Periphery 0.0048 0.0008 0.0028 0.0045 0.0058 0.0028 0.0034 0.0008 0.0025 0.0192 0.0054 0.0021 0.0015 0.0005 0.0022 0.0079 0.002 0.0014

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 22.168 17.3048 31.8952 38.5136 25.0985 28.0847 28.503 40.38 41.3606 37.1828
Translational Efficiency 1.4267 1.728 0.9309 0.9135 0.9748 1.1742 1.1777 0.6727 0.7514 0.6609

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✔
Gene Expression ✔
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
2662 1182 1360 641 1668 1683 1446 134 4330 2865 2806 775

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 1064.99 1037.63 1608.25 1401.07 1203.87 1214.47 1378.01 1579.11 1118.49 1141.51 1489.60 1431.85
Standard Deviation 134.75 212.87 273.01 248.58 164.99 184.08 179.92 262.37 161.92 214.90 256.99 259.89
Intensity Change Log 2 — -0.037548 0.594652 0.395689 — 0.012647 0.194907 0.391432 — -0.010697 0.396372 0.393432

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000084 0.000266 0.000380 0.001673 0.000188 0.000191 0.000190 0.002159 0.000124 0.000222 0.000282 0.001757
Bud Neck 0.000378 0.000663 0.001391 0.003555 0.000249 0.000590 0.001393 0.004341 0.000328 0.000620 0.001392 0.003691
Bud Site 0.013311 0.023761 0.010352 0.056392 0.016235 0.017584 0.013454 0.047979 0.014438 0.020132 0.011950 0.054937
Cell Periphery 0.000159 0.000211 0.000867 0.002990 0.000277 0.000135 0.000135 0.002034 0.000204 0.000167 0.000490 0.002824
Cytoplasm 0.830417* 0.833297* 0.886918* 0.803360* 0.820120* 0.778648* 0.614823* 0.724352* 0.826451* 0.801194* 0.746701* 0.789700*
Cytoplasmic Foci 0.110355 0.079631 0.020619 0.046771 0.107797 0.123537 0.061181 0.071486 0.109369 0.105423 0.041522 0.051044
Eisosomes 0.000005 0.000005 0.000002 0.000011 0.000003 0.000004 0.000005 0.000015 0.000004 0.000004 0.000004 0.000011
Endoplasmic Reticulum 0.002313 0.002179 0.002081 0.000531 0.007195 0.004169 0.004344 0.000251 0.004194 0.003348 0.003247 0.000483
Endosome 0.002673 0.007139 0.004238 0.004270 0.004288 0.008041 0.013363 0.009669 0.003295 0.007669 0.008940 0.005204
Golgi 0.014246 0.009592 0.014098 0.004073 0.013863 0.012878 0.002858 0.006724 0.014098 0.011522 0.008306 0.004531
Lipid Particles 0.000444 0.001043 0.004449 0.010124 0.000838 0.000683 0.002440 0.023907 0.000596 0.000831 0.003414 0.012507
Mitochondria 0.001052 0.000500 0.000052 0.000107 0.000395 0.001379 0.000094 0.000641 0.000799 0.001016 0.000073 0.000199
Mitotic Spindle 0.000331 0.000977 0.003794 0.001197 0.000518 0.001916 0.001917 0.003249 0.000403 0.001528 0.002826 0.001551
None 0.001809 0.002381 0.000551 0.000938 0.000331 0.000822 0.000827 0.000095 0.001240 0.001465 0.000694 0.000792
Nuclear Periphery 0.000022 0.000145 0.000085 0.000019 0.000024 0.000049 0.000149 0.000036 0.000023 0.000088 0.000118 0.000022
Nuclear Periphery Foci 0.000211 0.001879 0.005797 0.001837 0.000969 0.000588 0.001552 0.003592 0.000503 0.001121 0.003609 0.002140
Nucleolus 0.000051 0.000305 0.000034 0.000088 0.000024 0.000062 0.000141 0.000081 0.000041 0.000162 0.000089 0.000087
Nucleus 0.002688 0.003726 0.006515 0.003111 0.001396 0.002752 0.007668 0.002455 0.002190 0.003154 0.007109 0.002997
Peroxisomes 0.000229 0.000477 0.000071 0.002250 0.000185 0.000369 0.000453 0.002830 0.000212 0.000414 0.000268 0.002351
Vacuole 0.007868 0.022346 0.032192 0.054186 0.010556 0.034328 0.263648* 0.093158 0.008904 0.029385 0.151467 0.060924
Vacuole Periphery 0.011355 0.009477 0.005517 0.002518 0.014547 0.011276 0.009366 0.000945 0.012585 0.010534 0.007500 0.002246

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -4.86 -9.52 -2.90 -2.58 -2.34 -0.13 -0.39 -2.48 -2.48 -2.47 -5.15 -8.53 -3.43 -3.23 -3.08
Bud Neck -4.23 -8.97 -6.89 -6.31 -4.61 -4.13 -14.49 -6.16 -5.69 -4.59 -5.19 -15.40 -8.47 -7.78 -5.86
Bud Site -6.70 3.07 -9.89 -7.23 -10.42 -1.14 2.03 -4.59 -4.40 -4.95 -6.29 2.98 -10.68 -9.10 -11.19
Cell Periphery -1.77 -8.31 -6.22 -6.10 -4.45 6.31 6.59 -5.06 -5.49 -5.50 1.97 -6.55 -6.88 -6.99 -6.03
Cytoplasm -0.36 -8.40 4.35 4.12 8.97 5.08 21.91 5.22 3.45 -3.33 4.65 13.44 5.94 3.10 -2.46
Cytoplasmic Foci 6.54 29.97 11.61 4.84 -7.41 -2.98 11.08 3.07 4.58 -1.66 1.12 26.35 12.02 10.33 -3.77
Eisosomes 0.85 8.76 -4.83 -4.87 -7.00 -2.21 -5.86 -3.17 -2.99 -2.72 0.55 2.82 -6.14 -6.18 -6.65
Endoplasmic Reticulum 0.29 0.47 5.88 4.45 3.87 2.40 2.64 7.27 4.78 5.79 1.30 1.88 8.83 5.59 6.52
Endosome -6.19 -4.61 -2.75 2.71 -0.19 -6.95 -14.27 -2.45 -0.78 1.42 -10.27 -15.13 -3.14 2.84 4.57
Golgi 6.81 0.04 12.40 6.36 8.64 1.07 17.60 4.06 3.38 -1.80 4.25 9.59 13.07 8.55 4.77
Lipid Particles -2.79 -13.20 -8.19 -7.61 -4.71 1.29 -8.83 -4.10 -4.13 -3.81 -2.26 -16.37 -8.60 -8.43 -6.54
Mitochondria 3.24 11.70 9.41 2.62 -0.84 -1.48 2.69 -0.33 1.07 -1.05 -0.55 10.61 5.65 2.11 -1.18
Mitotic Spindle -2.70 -6.55 -4.24 -1.30 4.12 -2.78 -1.99 -1.56 -0.66 -0.66 -3.62 -5.55 -3.73 -0.37 2.04
None -0.64 5.09 1.39 1.39 -0.58 -0.98 -0.71 6.50 1.46 1.09 -0.47 1.63 0.91 1.04 -0.16
Nuclear Periphery -1.11 -4.85 -0.34 1.07 8.32 -1.35 -7.01 -2.54 0.10 5.63 -1.39 -8.06 -0.88 1.25 9.28
Nuclear Periphery Foci -3.46 -12.25 -8.51 -0.31 7.62 1.08 -1.84 -4.51 -5.52 -3.95 -2.43 -11.75 -7.87 -4.34 4.04
Nucleolus -1.70 5.22 -4.97 1.41 -6.89 -2.89 -15.48 -6.47 -1.24 5.18 -1.95 -11.26 -7.05 1.14 -0.32
Nucleus -2.74 -9.95 -2.46 -0.58 3.85 -3.67 -13.57 -4.86 0.05 9.41 -3.57 -16.15 -3.51 -1.31 6.12
Peroxisomes -3.44 13.76 -7.50 -6.37 -8.07 -5.48 -8.56 -3.98 -3.71 -3.59 -5.70 -3.36 -8.53 -7.67 -8.29
Vacuole -8.18 -10.61 -12.90 -8.79 -5.81 -10.82 -36.63 -8.32 -6.29 10.90 -14.10 -33.14 -15.00 -9.54 13.77
Vacuole Periphery 1.57 5.93 7.87 5.51 2.85 2.09 3.98 10.43 11.57 13.27* 2.20 6.64 10.59 8.66 6.06
Standard name
Human Ortholog
Description Sensor of mitochondrial dysfunction; regulates the subcellular location of Rtg1p and Rtg3p, transcriptional activators of the retrograde (RTG) and TOR pathways; Rtg2p is inhibited by the phosphorylated form of Mks1p
Localization
Cell Percentages cytoplasm (100%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-7

Rtg2

Rtg2


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Rtg2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available