Standard name | |
---|---|
Human Ortholog | |
Description | Karyopherin, responsible for nuclear import of specific proteins; cargoes include Spt15p, Sua7p, histones H2A and H2B, and Nap1p; amino terminus shows similarity to those of other importins, particularly Cse1p; localization is primarily nuclear; function is regulated by sumoylation; protein abundance increases in response to DNA replication stress |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 1 | 2 | 2 |
Bud | 0 | 0 | 2 | 5 | 1 | 1 | 3 | 7 | 5 | 9 | 8 | 5 | 0 | 0 | 3 | 0 | 0 | 0 | 2 | 7 | 1 | 6 | 9 | 15 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 3 | 1 | 2 | 1 | 3 |
Bud Site | 0 | 0 | 1 | 1 | 0 | 4 | 0 | 4 | 4 | 2 | 2 | 4 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 185 | 231 | 210 | 129 | 179 | 217 | 309 | 276 | 146 | 192 | 139 | 178 | 81 | 155 | 163 | 27 | 21 | 30 | 164 | 198 | 233 | 112 | 89 | 92 |
Endoplasmic Reticulum | 3 | 7 | 1 | 0 | 0 | 0 | 0 | 2 | 1 | 2 | 0 | 0 | 0 | 0 | 0 | 2 | 3 | 1 | 0 | 1 | 4 | 1 | 2 | 2 |
Endosome | 0 | 0 | 4 | 2 | 15 | 3 | 5 | 2 | 0 | 0 | 0 | 0 | 1 | 2 | 2 | 1 | 1 | 1 | 4 | 5 | 1 | 3 | 3 | 3 |
Golgi | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 1 | 0 | 1 | 1 |
Mitochondria | 0 | 11 | 15 | 101 | 37 | 138 | 133 | 220 | 160 | 251 | 201 | 271 | 1 | 0 | 6 | 4 | 0 | 2 | 0 | 10 | 7 | 1 | 3 | 1 |
Nucleus | 19 | 39 | 24 | 44 | 36 | 74 | 89 | 103 | 32 | 72 | 34 | 55 | 19 | 25 | 11 | 4 | 6 | 1 | 42 | 109 | 84 | 53 | 49 | 69 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3 | 5 | 11 | 1 | 4 | 5 |
Vac/Vac Membrane | 78 | 133 | 100 | 70 | 127 | 67 | 98 | 99 | 28 | 50 | 42 | 57 | 86 | 116 | 126 | 33 | 21 | 24 | 12 | 49 | 61 | 28 | 26 | 47 |
Unique Cell Count | 229 | 345 | 323 | 290 | 343 | 421 | 531 | 583 | 289 | 430 | 327 | 430 | 162 | 262 | 271 | 62 | 40 | 44 | 237 | 403 | 418 | 216 | 200 | 252 |
Labelled Cell Count | 286 | 423 | 357 | 352 | 395 | 507 | 637 | 716 | 376 | 579 | 428 | 571 | 188 | 298 | 312 | 73 | 52 | 59 | 237 | 403 | 418 | 216 | 200 | 252 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 7.1 | 5.1 | 4.7 | 4.4 | 4.9 | 4.4 | 4.3 | 4.2 | 3.6 | 3.8 | 3.7 | 3.6 | 6.1 | 6.4 | 5.5 | 7.9 | 7.9 | 9.7 | 6.2 | 6.6 | 7.0 |
Std Deviation (1e-4) | 0.8 | 0.7 | 1.1 | 1.0 | 1.3 | 1.1 | 1.5 | 0.8 | 0.8 | 1.6 | 1.1 | 1.0 | 1.0 | 1.5 | 1.2 | 2.0 | 1.0 | 2.5 | 1.3 | 1.5 | 2.0 |
Intensity Change (Log2) | – | – | – | -0.08 | 0.07 | -0.09 | -0.1 | -0.14 | -0.37 | -0.31 | -0.32 | -0.35 | 0.39 | 0.46 | 0.25 | 0.76 | 0.76 | 1.06 | 0.42 | 0.51 | 0.58 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | -5.1 | -3.4 | -3.7 | -2.0 | -5.1 | -3.6 | -5.5 | -5.8 | -6.4 | -3.2 | -1.5 | -1.2 | -3.2 | -1.6 | 0.4 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | -2.3 | -3.5 | -1.6 | 0 | 0 | 0 |
Nucleus | 3.0 | 1.4 | 4.1 | 3.9 | 4.3 | 1.6 | 3.8 | 1.3 | 2.4 | 1.6 | 0.9 | -1.7 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | -1.9 | 1.7 | -4.9 | -4.2 | -4.9 | -6.5 | -6.6 | -5.6 | -5.9 | 4.7 | 3.3 | 3.9 | 3.4 | 2.7 | 3.1 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 2.6628 | 3.4463 | 3.1726 | 2.7824 | 2.4558 | 3.1392 | 1.2114 | 1.814 | 1.853 | 1.4261 | 1.4723 | 1.4823 | 5.024 | 6.0616 | 5.6757 | 5.5 | 5.1261 | 5.4458 |
Actin | 0.0182 | 0.0005 | 0.013 | 0.0005 | 0.023 | 0.0029 | 0.0083 | 0.0017 | 0.0042 | 0.0006 | 0.0019 | 0.0005 | 0.009 | 0.0004 | 0.0096 | 0.0004 | 0.0005 | 0.0006 |
Bud | 0.0017 | 0.0004 | 0.0009 | 0.0001 | 0.0049 | 0.0006 | 0.001 | 0.001 | 0.0006 | 0.0009 | 0.0012 | 0.0003 | 0.0017 | 0.0002 | 0.0003 | 0.0002 | 0.0001 | 0.0004 |
Bud Neck | 0.0083 | 0.0006 | 0.0056 | 0.0013 | 0.0019 | 0.0091 | 0.0082 | 0.0008 | 0.002 | 0.0008 | 0.0061 | 0.0027 | 0.0011 | 0.001 | 0.0029 | 0.001 | 0.0011 | 0.0053 |
Bud Periphery | 0.002 | 0.0002 | 0.001 | 0.0001 | 0.0164 | 0.0005 | 0.0012 | 0.0006 | 0.0007 | 0.0005 | 0.0022 | 0.0001 | 0.001 | 0.0001 | 0.0002 | 0.0001 | 0 | 0.0001 |
Bud Site | 0.0059 | 0.0016 | 0.005 | 0.0004 | 0.0029 | 0.0013 | 0.0024 | 0.0019 | 0.0005 | 0.0004 | 0.0029 | 0.0002 | 0.0024 | 0.0017 | 0.0018 | 0.0003 | 0.0004 | 0.0002 |
Cell Periphery | 0.0003 | 0.0001 | 0.0007 | 0.0002 | 0.0003 | 0.0001 | 0.0003 | 0 | 0 | 0 | 0.0001 | 0 | 0.0001 | 0.0001 | 0.0001 | 0 | 0 | 0 |
Cytoplasm | 0.6092 | 0.6096 | 0.6318 | 0.7444 | 0.5242 | 0.5326 | 0.3857 | 0.4275 | 0.3694 | 0.4907 | 0.3921 | 0.3363 | 0.6135 | 0.5967 | 0.5997 | 0.6486 | 0.7775 | 0.5458 |
Cytoplasmic Foci | 0.0198 | 0.0023 | 0.0118 | 0.0058 | 0.0212 | 0.0081 | 0.0049 | 0.003 | 0.002 | 0.0015 | 0.0192 | 0.0044 | 0.0157 | 0.0036 | 0.0046 | 0.0043 | 0.0037 | 0.0114 |
Eisosomes | 0.0002 | 0 | 0.0003 | 0 | 0.0002 | 0 | 0.0002 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0.0002 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0.0134 | 0.0072 | 0.009 | 0.0213 | 0.0112 | 0.0052 | 0.0094 | 0.0043 | 0.0035 | 0.0072 | 0.004 | 0.0043 | 0.0072 | 0.0035 | 0.0047 | 0.0087 | 0.0057 | 0.0047 |
Endosome | 0.0171 | 0.0031 | 0.0142 | 0.0217 | 0.0497 | 0.0124 | 0.0137 | 0.0042 | 0.0034 | 0.0038 | 0.0088 | 0.0059 | 0.0071 | 0.0038 | 0.0031 | 0.004 | 0.0038 | 0.0037 |
Golgi | 0.005 | 0.0001 | 0.0067 | 0.0025 | 0.0197 | 0.0036 | 0.0011 | 0.0004 | 0.0002 | 0 | 0.0011 | 0.0001 | 0.0017 | 0.0001 | 0.0004 | 0.0001 | 0.0001 | 0.0002 |
Lipid Particles | 0.0071 | 0.0001 | 0.0062 | 0.0184 | 0.0088 | 0.0019 | 0.0019 | 0.0004 | 0.0001 | 0 | 0.0013 | 0.0001 | 0.0007 | 0.0001 | 0.0015 | 0.0002 | 0 | 0.0003 |
Mitochondria | 0.016 | 0.0006 | 0.0078 | 0.0012 | 0.0302 | 0.0065 | 0.0039 | 0.0007 | 0.0007 | 0.0005 | 0.002 | 0.0004 | 0.0012 | 0.0003 | 0.0004 | 0.0007 | 0.0004 | 0.0004 |
None | 0.0488 | 0.0512 | 0.0417 | 0.0231 | 0.0444 | 0.0229 | 0.0191 | 0.0121 | 0.0121 | 0.0059 | 0.0053 | 0.006 | 0.0792 | 0.0406 | 0.09 | 0.1192 | 0.0226 | 0.0597 |
Nuclear Periphery | 0.0179 | 0.0084 | 0.0126 | 0.0137 | 0.0217 | 0.0102 | 0.0507 | 0.0233 | 0.0307 | 0.0306 | 0.0141 | 0.0292 | 0.0177 | 0.0098 | 0.0089 | 0.0209 | 0.0113 | 0.0105 |
Nucleolus | 0.0044 | 0.0005 | 0.0011 | 0.0008 | 0.0022 | 0.0007 | 0.0063 | 0.0009 | 0.0007 | 0.0005 | 0.003 | 0.0009 | 0.0007 | 0.0005 | 0.0005 | 0.0006 | 0.0002 | 0.0005 |
Nucleus | 0.1771 | 0.3079 | 0.2085 | 0.1337 | 0.1707 | 0.3637 | 0.4519 | 0.5035 | 0.5592 | 0.4456 | 0.486 | 0.575 | 0.2152 | 0.3264 | 0.2522 | 0.1837 | 0.1662 | 0.3413 |
Peroxisomes | 0.0104 | 0.0001 | 0.0105 | 0.0009 | 0.0149 | 0.0035 | 0.0004 | 0.0006 | 0.0001 | 0.0001 | 0.0027 | 0.0001 | 0.0079 | 0.0001 | 0.0105 | 0.0001 | 0.0001 | 0.0015 |
Punctate Nuclear | 0.0113 | 0.0031 | 0.007 | 0.0045 | 0.0214 | 0.0104 | 0.0217 | 0.0098 | 0.0069 | 0.0062 | 0.0414 | 0.0307 | 0.014 | 0.0082 | 0.007 | 0.0041 | 0.0047 | 0.0116 |
Vacuole | 0.0054 | 0.0023 | 0.004 | 0.0047 | 0.0068 | 0.0029 | 0.0056 | 0.0028 | 0.0021 | 0.0033 | 0.0037 | 0.0022 | 0.0024 | 0.0026 | 0.0013 | 0.0023 | 0.0012 | 0.0014 |
Vacuole Periphery | 0.001 | 0.0003 | 0.0008 | 0.0009 | 0.0033 | 0.0007 | 0.0019 | 0.0005 | 0.0007 | 0.001 | 0.0007 | 0.0007 | 0.0007 | 0.0002 | 0.0002 | 0.0005 | 0.0003 | 0.0003 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 23.7882 | 11.7776 | 9.9533 | 17.7877 | 17.0819 | 17.9227 | 15.0508 | 15.4049 | 15.9076 | 22.0446 |
Translational Efficiency | 0.9825 | 0.9041 | 1.2886 | 0.7259 | 0.9391 | 1.1867 | 0.8329 | 0.7168 | 0.7566 | 0.7746 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✔ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
363 | 467 | 66 | 1138 | 1731 | 1576 | 174 | 1067 | 2094 | 2043 | 240 | 2205 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 681.56 | 791.02 | 1139.94 | 886.48 | 722.16 | 811.50 | 1036.92 | 949.32 | 715.12 | 806.82 | 1065.25 | 916.89 |
Standard Deviation | 71.80 | 103.43 | 156.20 | 125.00 | 85.22 | 102.04 | 121.69 | 135.52 | 84.46 | 102.72 | 139.86 | 133.93 |
Intensity Change Log 2 | — | 0.214874 | 0.742045 | 0.379247 | — | 0.168273 | 0.521914 | 0.394576 | — | 0.191087 | 0.632993 | 0.387154 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.000109 | 0.000321 | 0.001999 | 0.001298 | 0.000068 | 0.000393 | 0.000648 | 0.001022 | 0.000075 | 0.000376 | 0.001020 | 0.001164 |
Bud Neck | 0.006478 | 0.006207 | 0.002943 | 0.009427 | 0.002357 | 0.009155 | 0.003131 | 0.015990 | 0.003072 | 0.008481 | 0.003079 | 0.012603 |
Bud Site | 0.003872 | 0.005806 | 0.004355 | 0.031147 | 0.001473 | 0.008692 | 0.003138 | 0.029973 | 0.001889 | 0.008033 | 0.003473 | 0.030579 |
Cell Periphery | 0.000172 | 0.000107 | 0.000401 | 0.000264 | 0.000089 | 0.000109 | 0.000274 | 0.000156 | 0.000104 | 0.000108 | 0.000309 | 0.000212 |
Cytoplasm | 0.488316* | 0.529867* | 0.172847* | 0.397628* | 0.511090* | 0.552300* | 0.130846* | 0.289143* | 0.507142* | 0.547172* | 0.142396* | 0.345132* |
Cytoplasmic Foci | 0.145097 | 0.068419 | 0.010135 | 0.019380 | 0.058035 | 0.087748 | 0.011063 | 0.003846 | 0.073127 | 0.083330 | 0.010807 | 0.011863 |
Eisosomes | 0.000108 | 0.000046 | 0.000098 | 0.000064 | 0.000030 | 0.000049 | 0.000116 | 0.000031 | 0.000043 | 0.000049 | 0.000111 | 0.000048 |
Endoplasmic Reticulum | 0.000774 | 0.002406 | 0.021084 | 0.002542 | 0.001018 | 0.001126 | 0.008904 | 0.004908 | 0.000975 | 0.001418 | 0.012253 | 0.003687 |
Endosome | 0.006324 | 0.006229 | 0.005134 | 0.016289 | 0.002149 | 0.007898 | 0.003317 | 0.004282 | 0.002873 | 0.007516 | 0.003817 | 0.010479 |
Golgi | 0.001069 | 0.000613 | 0.000327 | 0.004419 | 0.000290 | 0.001902 | 0.000135 | 0.004063 | 0.000425 | 0.001608 | 0.000188 | 0.004247 |
Lipid Particles | 0.005691 | 0.002359 | 0.003491 | 0.003809 | 0.002138 | 0.002554 | 0.003432 | 0.000948 | 0.002754 | 0.002509 | 0.003448 | 0.002424 |
Mitochondria | 0.004072 | 0.002401 | 0.000809 | 0.008147 | 0.002469 | 0.004259 | 0.000711 | 0.005889 | 0.002747 | 0.003834 | 0.000738 | 0.007054 |
Mitotic Spindle | 0.003441 | 0.006274 | 0.001356 | 0.097240 | 0.002547 | 0.007769 | 0.002643 | 0.044316 | 0.002702 | 0.007427 | 0.002289 | 0.071630 |
None | 0.005722 | 0.006417 | 0.002397 | 0.019479 | 0.007018 | 0.003833 | 0.007865 | 0.007300 | 0.006793 | 0.004424 | 0.006361 | 0.013586 |
Nuclear Periphery | 0.000599 | 0.000675 | 0.002059 | 0.002061 | 0.000749 | 0.000681 | 0.002121 | 0.002052 | 0.000723 | 0.000680 | 0.002104 | 0.002057 |
Nuclear Periphery Foci | 0.000676 | 0.002096 | 0.005570 | 0.004098 | 0.000627 | 0.000862 | 0.002084 | 0.002603 | 0.000636 | 0.001144 | 0.003043 | 0.003375 |
Nucleolus | 0.001371 | 0.002365 | 0.000894 | 0.003088 | 0.000984 | 0.000950 | 0.002859 | 0.000468 | 0.001051 | 0.001273 | 0.002318 | 0.001820 |
Nucleus | 0.282309* | 0.299819* | 0.685615* | 0.276525* | 0.389519* | 0.267975* | 0.740077* | 0.505074* | 0.370934* | 0.275254* | 0.725100* | 0.387120* |
Peroxisomes | 0.003193 | 0.002782 | 0.000557 | 0.002210 | 0.000858 | 0.002835 | 0.000483 | 0.000355 | 0.001262 | 0.002823 | 0.000503 | 0.001312 |
Vacuole | 0.039883 | 0.052967 | 0.077557 | 0.095965 | 0.016165 | 0.038052 | 0.075857 | 0.072019 | 0.020277 | 0.041462 | 0.076325 | 0.084377 |
Vacuole Periphery | 0.000727 | 0.001823 | 0.000375 | 0.004921 | 0.000327 | 0.000858 | 0.000296 | 0.005562 | 0.000396 | 0.001079 | 0.000318 | 0.005231 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -3.46 | -7.72 | -3.80 | -2.67 | 4.35 | -3.24 | -9.44 | -4.23 | -2.57 | 0.40 | -3.88 | -11.75 | -5.86 | -3.35 | 2.79 |
Bud Neck | -0.01 | 1.73 | -0.96 | -1.07 | -4.82 | -6.14 | -2.25 | -6.31 | -0.56 | -4.04 | -5.87 | -1.58 | -8.03 | -0.41 | -5.41 |
Bud Site | -0.82 | -0.45 | -4.18 | -4.14 | -5.16 | -5.48 | -2.57 | -5.86 | -2.20 | -3.58 | -5.45 | -2.80 | -8.50 | -3.96 | -5.68 |
Cell Periphery | 4.10 | -5.08 | 0.65 | -2.81 | 5.27 | -2.00 | -7.14 | -7.08 | -4.93 | 4.45 | -0.59 | -9.01 | -5.01 | -4.62 | 6.40 |
Cytoplasm | -0.92 | 14.06 | 13.29 | 14.08 | -6.67 | -3.58 | 31.47 | 33.35 | 35.73 | -7.85 | -3.42 | 33.90 | 33.05 | 35.50 | -11.95 |
Cytoplasmic Foci | 8.48 | 17.23 | 17.41 | 12.12 | -0.76 | -6.85 | 17.79 | 20.99 | 24.36 | 3.34 | -2.57 | 23.37 | 25.01 | 26.30 | 1.03 |
Eisosomes | 7.31 | -0.28 | 4.71 | -3.28 | 3.33 | -6.84 | -10.77 | -8.01 | 0.88 | 8.81 | -2.52 | -10.62 | -4.81 | -2.16 | 8.58 |
Endoplasmic Reticulum | -4.73 | -8.15 | -12.52 | -5.06 | 6.72 | -1.09 | -10.40 | -18.30 | -16.91 | 6.28 | -3.85 | -12.98 | -22.58 | -17.90 | 9.29 |
Endosome | 0.11 | 0.83 | 0.63 | 0.50 | -0.19 | -7.68 | -3.10 | 0.68 | 8.28 | 3.83 | -6.89 | -2.57 | -1.38 | 4.87 | 0.74 |
Golgi | 0.74 | 1.15 | -0.25 | -1.86 | -2.80 | -3.58 | 2.20 | -1.15 | 1.32 | -1.41 | -3.17 | 1.82 | -1.98 | 0.99 | -2.85 |
Lipid Particles | 3.29 | 2.01 | 3.24 | 0.49 | 1.87 | -1.35 | -2.93 | 4.29 | 4.65 | 5.21 | 0.92 | -1.59 | 2.93 | 2.27 | 3.97 |
Mitochondria | 0.76 | 1.39 | 0.36 | -0.81 | -3.20 | -1.36 | 2.09 | 0.20 | 1.75 | -2.96 | -0.86 | 2.50 | -0.01 | 1.04 | -4.32 |
Mitotic Spindle | -0.78 | 1.16 | -8.70 | -8.07 | -9.98 | -2.77 | 0.15 | -7.02 | -5.46 | -6.97 | -2.86 | 0.61 | -11.54 | -9.74 | -11.80 |
None | -0.26 | 3.72 | -3.82 | -4.26 | -7.84 | 6.18 | 0.89 | 1.38 | -5.29 | -0.41 | 5.28 | 1.79 | -2.75 | -6.92 | -3.19 |
Nuclear Periphery | -0.78 | -6.49 | -12.03 | -15.42 | -2.89 | 0.52 | -9.11 | -17.37 | -16.77 | -1.18 | 0.27 | -10.91 | -20.88 | -20.82 | -2.27 |
Nuclear Periphery Foci | -3.39 | -4.92 | -2.47 | 0.33 | 3.21 | -2.05 | -5.74 | -4.97 | -3.95 | 1.48 | -3.93 | -7.38 | -4.50 | -2.55 | 3.04 |
Nucleolus | -1.52 | 1.74 | 0.00 | 1.44 | -1.45 | 0.40 | -1.12 | 4.89 | 6.09 | 1.95 | -1.16 | -0.87 | 0.62 | 1.40 | 1.08 |
Nucleus | -1.62 | -12.39 | -8.36 | -6.66 | 8.84 | 13.43 | -14.06 | -14.05 | -25.21 | 6.13 | 11.53 | -17.80 | -11.85 | -23.08 | 11.70 |
Peroxisomes | 0.33 | 2.87 | 2.49 | 2.52 | -1.31 | -5.15 | 3.96 | 5.45 | 7.21 | 2.45 | -4.03 | 4.46 | 3.47 | 6.62 | -0.78 |
Vacuole | -4.12 | -4.01 | -17.91 | -12.61 | -3.59 | -11.18 | -8.88 | -21.22 | -16.50 | -0.39 | -11.87 | -9.93 | -30.63 | -23.15 | -2.26 |
Vacuole Periphery | -0.93 | 1.18 | -1.56 | 0.16 | -3.04 | -3.54 | 0.33 | -1.59 | -0.70 | -1.65 | -2.56 | 0.85 | -2.78 | -0.85 | -3.10 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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