Standard name
Human Ortholog
Description Protein required for synthesis of diphthamide; required along with Dph1p, Kti11p, Jjj3p, and Dph5p; diphthamide is a modified histidine residue of translation elongation factor 2 (Eft1p or Eft2p); may act in a complex with Dph1p and Kti11p
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – – – –
Cell Periphery 0.06 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0
Cytoplasm 0.98 0.97 0.98 0.97 0.96 0.9 0.83 0.82 0.75 0.74 0.66 0.71 0.99 1.0 1.0 0.96 – – 0.93 0.95 0.92 0.97 0.94 0.93
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0
Mitochondria 0.05 0 0 0.06 0.05 0.22 0.26 0.24 0.35 0.43 0.5 0.46 0 0 0 0 – – 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 1 0 0 0 1 0 0 0 1 1 1 1 0 0 0 0 – – 0 0 1 0 0 0
Bud 0 0 3 1 1 8 2 4 2 6 0 0 0 0 0 0 – – 2 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 – – – – – – – –
Cell Periphery 9 9 2 5 8 4 9 8 9 13 5 5 2 6 10 2 – – 0 0 0 0 0 0
Cytoplasm 158 257 186 148 328 250 348 399 222 312 95 114 153 259 257 52 – – 176 257 180 139 175 147
Endoplasmic Reticulum 0 3 0 0 1 0 3 4 5 3 3 2 2 1 0 1 – – 3 2 2 1 2 2
Endosome 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 – – 1 0 0 0 0 1
Golgi 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 – – 1 1 0 0 0 0
Mitochondria 8 1 1 9 16 61 110 117 104 182 71 74 0 0 0 2 – – 0 1 1 0 1 1
Nucleus 0 3 1 1 0 1 3 3 0 1 0 0 0 0 1 1 – – 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 1 0 1 1 0 3 0 0 0 0 0 0 – – 0 0 1 0 0 0
Nucleolus 0 1 0 0 0 0 1 1 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0
Vac/Vac Membrane 1 0 2 1 3 1 4 13 4 8 1 3 0 1 0 0 – – 0 4 3 1 1 3
Unique Cell Count 162 265 190 153 342 277 421 488 295 423 143 160 155 260 258 54 190 270 196 144 187 158
Labelled Cell Count 177 274 195 165 359 326 481 552 347 530 176 199 157 267 268 58 190 270 196 144 187 158


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 9.3 10.1 7.7 7.0 6.5 5.3 5.0 5.4 4.8 4.7 4.4 4.5 9.7 9.1 8.9 13.8 – – 9.7 9.5 10.4
Std Deviation (1e-4) 1.1 1.4 1.1 1.4 1.0 1.2 1.2 1.2 0.9 1.0 0.9 1.2 0.9 1.2 1.2 2.8 – – 1.3 1.4 1.6
Intensity Change (Log2) – – – -0.14 -0.24 -0.53 -0.63 -0.52 -0.68 -0.7 -0.8 -0.76 0.33 0.24 0.21 0.84 – – 0.34 0.3 0.43


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 – –
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 – –
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 – –
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 1.8 0 – –
Cytoplasm -0.7 -1.2 -3.3 -5.3 -5.5 -6.7 -7.1 -7.8 -7.1 0.6 1.7 1.7 -0.7 – –
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 – –
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 – –
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 – –
Mitochondria 0 2.6 6.7 7.6 7.2 0 0 0 0 0 0 0 0 – –
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 – –
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 – –
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 – –
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 – –
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 – –
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 4.6066 6.4362 5.763 4.9909 5.444 5.5905 4.6456 6.8596 5.5321 5.1585 4.7608 5.8665 6.0443 6.9046 7.3375 6.8675 6.4977 6.96
Actin 0.036 0.0017 0.0022 0.0006 0.0032 0.0004 0.0053 0.0038 0.0752 0.0091 0.0037 0.0038 0.0403 0.0003 0.0037 0.0006 0.0002 0.0118
Bud 0.0006 0.0005 0.0024 0.0006 0.0003 0.0022 0.0004 0.0003 0.0012 0.0003 0.0001 0.0002 0.0002 0.0003 0.0004 0.0003 0.0001 0.0003
Bud Neck 0.0031 0.0002 0.0007 0.0003 0.0005 0.0009 0.0002 0.0002 0.002 0.0003 0.0004 0.0005 0.0104 0.0002 0.0005 0.0002 0.0002 0.0009
Bud Periphery 0.0009 0.0002 0.0015 0.0001 0.0002 0.0008 0.0005 0.0003 0.0013 0.0001 0.0001 0.0001 0.0002 0.0001 0.0002 0.0002 0 0.0002
Bud Site 0.0061 0.0017 0.0079 0.0005 0.0006 0.0002 0.0006 0.0017 0.0078 0.0004 0.0003 0.0006 0.0039 0.0011 0.0051 0.0002 0 0.001
Cell Periphery 0.0009 0.0005 0.0004 0.0001 0.0001 0.001 0.0004 0.0002 0.0005 0.0001 0.0001 0.0001 0.0002 0.0002 0.0003 0.0001 0 0.0001
Cytoplasm 0.7572 0.9572 0.9084 0.9243 0.8911 0.9641 0.7762 0.9321 0.6812 0.8403 0.8943 0.9269 0.6729 0.9733 0.8391 0.9712 0.9683 0.9039
Cytoplasmic Foci 0.0259 0.0052 0.0201 0.0152 0.0211 0.0075 0.0067 0.006 0.0299 0.037 0.0312 0.0149 0.0299 0.0069 0.0292 0.005 0.0029 0.0173
Eisosomes 0.0008 0 0.0001 0 0 0 0.0001 0.0001 0.0009 0 0 0 0.0001 0 0.0001 0 0 0.0001
Endoplasmic Reticulum 0.0087 0.006 0.0048 0.0062 0.0028 0.0055 0.0238 0.0032 0.0043 0.0308 0.007 0.0027 0.0109 0.0031 0.0046 0.0031 0.0012 0.0028
Endosome 0.0173 0.0099 0.0114 0.0338 0.0203 0.0075 0.0536 0.0061 0.0367 0.0323 0.0144 0.0089 0.0654 0.0085 0.013 0.0055 0.0017 0.0173
Golgi 0.0159 0.001 0.0018 0.0011 0.0105 0.0011 0.005 0.0049 0.0135 0.0104 0.0236 0.0009 0.027 0.001 0.0065 0.0003 0.0002 0.0172
Lipid Particles 0.0229 0.0002 0.0011 0.0001 0.0081 0.0001 0.0014 0.001 0.0216 0.008 0.0036 0.0007 0.0114 0.0001 0.0035 0.0001 0 0.0076
Mitochondria 0.0188 0.0003 0.012 0.0004 0.0209 0.001 0.0041 0.0326 0.0076 0.0008 0.0011 0.0002 0.005 0.0001 0.0012 0.0001 0.0001 0.0108
None 0.0485 0.0103 0.019 0.0026 0.0012 0.0045 0.1082 0.0046 0.0657 0.0126 0.0042 0.0372 0.0998 0.0019 0.0848 0.0092 0.0239 0.0016
Nuclear Periphery 0.0018 0.0013 0.0007 0.0011 0.0004 0.0004 0.0069 0.0003 0.0029 0.0026 0.0004 0.0003 0.0048 0.0004 0.0006 0.0007 0.0001 0.0003
Nucleolus 0.0036 0 0.0001 0 0.0001 0.0001 0.0001 0 0.0033 0 0 0 0.0002 0 0.0001 0 0 0
Nucleus 0.0038 0.0008 0.0009 0.001 0.0006 0.0007 0.0012 0.0004 0.0058 0.0011 0.0004 0.0005 0.0013 0.0006 0.0006 0.0008 0.0004 0.0007
Peroxisomes 0.0167 0.0001 0.0006 0.0002 0.0151 0.0001 0.0002 0.0009 0.0207 0.0087 0.0142 0.0004 0.0107 0 0.0044 0 0 0.0044
Punctate Nuclear 0.0061 0.0002 0.0005 0.0002 0.0003 0.0001 0.0004 0.0001 0.0104 0.0014 0.0002 0.0004 0.0019 0.0001 0.0006 0.0002 0.0001 0.0003
Vacuole 0.0037 0.0024 0.0029 0.0098 0.0019 0.0014 0.0032 0.0008 0.0057 0.0032 0.0006 0.0007 0.0027 0.0016 0.0014 0.0019 0.0003 0.0011
Vacuole Periphery 0.0007 0.0004 0.0005 0.0016 0.0006 0.0003 0.0018 0.0004 0.0016 0.0005 0.0002 0.0001 0.0008 0.0002 0.0002 0.0003 0.0001 0.0004

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 74.9353 77.4351 52.4438 52.6538 57.0462 43.2872 51.2643 47.8108 42.0326 55.2999
Translational Efficiency 0.6587 0.5848 0.8142 0.6267 0.7013 0.7643 0.6254 0.5101 0.5529 0.5575

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
2057 1648 97 1264 1959 2178 209 1409 4016 3826 306 2673

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 935.94 1010.26 1302.00 1222.77 957.18 1002.69 1364.62 1162.59 946.30 1005.95 1344.77 1191.05
Standard Deviation 114.67 144.59 144.69 163.27 116.26 141.68 158.88 163.42 115.93 142.99 157.25 166.09
Intensity Change Log 2 — 0.110239 0.476241 0.385665 — 0.067013 0.511637 0.280480 — 0.088545 0.494246 0.333441

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000161 0.000189 0.000662 0.001189 0.000167 0.000259 0.000455 0.000449 0.000164 0.000229 0.000520 0.000799
Bud Neck 0.002624 0.001396 0.002698 0.001778 0.002243 0.002095 0.001355 0.002515 0.002438 0.001794 0.001781 0.002166
Bud Site 0.011826 0.019984 0.017945 0.023965 0.014130 0.021505 0.015379 0.019693 0.012950 0.020850 0.016192 0.021713
Cell Periphery 0.000185 0.000098 0.000211 0.000109 0.000127 0.000087 0.000114 0.000070 0.000157 0.000092 0.000145 0.000088
Cytoplasm 0.793535* 0.790556* 0.460548* 0.786757* 0.728047* 0.754032* 0.757821* 0.680316* 0.761590* 0.769764* 0.663587* 0.730650*
Cytoplasmic Foci 0.104525 0.108550 0.114357 0.054056 0.167961 0.142991 0.065053 0.042570 0.135469 0.128156 0.080682 0.048001
Eisosomes 0.000026 0.000010 0.000045 0.000009 0.000030 0.000015 0.000013 0.000008 0.000028 0.000013 0.000023 0.000008
Endoplasmic Reticulum 0.001319 0.001091 0.006831 0.000188 0.001401 0.000926 0.000399 0.000329 0.001359 0.000997 0.002438 0.000262
Endosome 0.004643 0.008235 0.026942 0.005077 0.011536 0.009405 0.004737 0.010064 0.008005 0.008901 0.011776 0.007706
Golgi 0.008384 0.005434 0.000868 0.003211 0.008502 0.012142 0.000512 0.002064 0.008441 0.009253 0.000625 0.002606
Lipid Particles 0.001105 0.000640 0.004796 0.000970 0.001365 0.000864 0.001335 0.000758 0.001232 0.000768 0.002432 0.000858
Mitochondria 0.000914 0.001109 0.000064 0.000171 0.000508 0.002848 0.000016 0.000590 0.000716 0.002099 0.000031 0.000392
Mitotic Spindle 0.000203 0.002834 0.001101 0.012934 0.000459 0.003179 0.003233 0.015160 0.000328 0.003031 0.002557 0.014108
None 0.007338 0.000914 0.000128 0.000565 0.000972 0.000880 0.002225 0.000527 0.004233 0.000895 0.001560 0.000545
Nuclear Periphery 0.000025 0.000026 0.000673 0.000093 0.000021 0.000025 0.000112 0.000101 0.000023 0.000025 0.000290 0.000097
Nuclear Periphery Foci 0.000407 0.000426 0.014764 0.002009 0.000476 0.000290 0.005444 0.001033 0.000441 0.000349 0.008398 0.001494
Nucleolus 0.000323 0.000178 0.000167 0.000137 0.000136 0.000149 0.000071 0.000311 0.000232 0.000162 0.000101 0.000228
Nucleus 0.016258 0.007500 0.014802 0.011103 0.007556 0.005127 0.013664 0.013076 0.012013 0.006149 0.014025 0.012143
Peroxisomes 0.000641 0.000891 0.000768 0.001599 0.000950 0.001675 0.000424 0.002058 0.000792 0.001337 0.000533 0.001841
Vacuole 0.042550 0.045368 0.329650* 0.092977 0.050653 0.037672 0.127373 0.205186* 0.046502 0.040987 0.191494 0.152125
Vacuole Periphery 0.003008 0.004572 0.001981 0.001104 0.002761 0.003834 0.000266 0.003122 0.002887 0.004152 0.000810 0.002168

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -0.59 -4.43 -2.36 -2.28 -0.74 -2.14 -4.91 -2.47 -1.38 0.18 -1.86 -6.19 -3.04 -2.60 -0.85
Bud Neck 2.89 -0.13 1.95 -1.12 2.06 0.70 3.44 -0.25 -0.90 -3.43 2.66 1.90 1.59 -1.26 -0.45
Bud Site -5.96 -3.35 -4.02 0.37 -0.24 -5.67 -0.41 -1.73 2.73 -1.10 -8.46 -2.57 -4.21 2.15 -1.29
Cell Periphery 8.54 -0.60 8.34 0.08 4.43 6.15 1.37 7.10 2.99 4.42 10.85 1.54 11.07 2.08 5.72
Cytoplasm 0.49 12.48 5.14 4.20 -10.87 -3.65 1.42 6.89 9.70 2.36 -1.64 8.89 8.96 9.79 -5.20
Cytoplasmic Foci -0.91 -0.26 14.78 13.86 6.45 4.86 18.72 30.53 26.41 4.85 2.12 12.69 32.42 28.98 7.11
Eisosomes 8.69 -3.95 9.23 0.80 7.69 6.94 7.00 11.27 9.23 4.85 10.62 1.67 14.58 7.69 8.06
Endoplasmic Reticulum 0.69 -2.55 4.31 5.01 3.29 1.68 6.05 6.69 2.88 1.75 1.63 -1.17 7.12 5.09 3.33
Endosome -5.49 -6.92 1.53 5.80 7.16 2.57 9.42 3.44 1.69 -3.31 -1.68 -2.03 3.39 4.57 3.74
Golgi 5.60 25.60 10.73 5.03 -2.15 -3.78 21.35 14.59 11.43 -3.23 -1.38 33.42 17.89 12.25 -3.76
Lipid Particles 6.39 -3.95 -0.68 -5.87 3.85 4.07 -0.52 5.07 0.56 3.67 6.16 -3.87 3.44 -2.43 4.74
Mitochondria -0.50 15.77 12.44 2.59 -1.21 -3.34 6.72 0.73 3.46 -3.66 -3.20 15.36 6.02 4.22 -3.96
Mitotic Spindle -2.95 -3.16 -4.58 -2.73 -4.04 -3.26 -3.35 -5.21 -3.70 -2.99 -4.48 -3.79 -6.93 -4.59 -4.20
None 9.74 12.12 11.48 1.42 -4.75 0.47 -0.61 3.02 3.25 1.31 9.63 5.30 11.72 2.87 1.08
Nuclear Periphery -0.07 -4.04 -4.62 -4.56 3.47 -0.67 -8.06 -8.44 -6.73 4.09 -0.56 -6.44 -8.58 -7.71 4.65
Nuclear Periphery Foci -0.42 -6.39 -12.27 -11.99 5.41 1.75 -7.69 -3.22 -4.92 6.78 1.57 -9.55 -10.18 -11.74 8.00
Nucleolus 1.84 4.04 9.83 0.72 0.31 -0.63 4.53 -2.22 -1.83 -3.07 1.87 7.70 0.81 -0.82 -2.88
Nucleus 13.72 -0.07 2.85 -8.41 1.03 7.27 -6.41 -9.57 -13.15 2.44 16.41 -4.47 -4.38 -15.69 2.75
Peroxisomes -1.65 -1.05 -1.99 -0.72 -1.48 -3.02 4.46 -1.79 0.25 -3.54 -3.68 3.94 -2.64 -0.06 -4.03
Vacuole -1.16 -11.93 -18.49 -17.40 8.21 6.15 -9.90 -26.45 -28.53 -3.94 3.35 -14.49 -31.61 -32.75 3.36
Vacuole Periphery -3.18 2.57 9.35 7.77 1.64 -2.35 16.14 1.19 2.56 -3.80 -3.76 11.01 4.72 6.37 -2.46
Protein Complexes26S Proteasome complex37S mitochondrial small ribosomal subunit54S mitochondrial large ribosomal subunit60S cytosolic large ribosomal subunitAar2-U5 snRNPAnaphase-Promoting Complex variant 1Anaphase-Promoting Complex variant 2Anaphase-Promoting Complex variant 3Anaphase-Promoting Complex variant 4Arp2/3 complexATG1 kinase complexBLOC-1 complexCcr4-Not complexCentral kinetochore CTF19 complexCleavage and polyadenylation specificity factorcomplexCMG helicase complexCMG-Pol epsilon complexCOMPASS complexCOP9 signalosome COPI vesicle coat complexCoQ6 biosynthesis complexCore mediator complexCORVET complexCTF18-RFC complexCURI complex variant 1CURI complex variant 2CURI complex variant 3Cytoplasmic exosome complex, DIS3 variantDASH complexDNA-directed RNA Polymerase I complexDNA-directed RNA polymerase II complexDNA-directed RNA polymerase III complexDNA replication factor C complexEGO complexELG1-RFC complex Elongator holoenzyme complexEndoplasmic Reticulum Membrane ComplexEukaryotic translation initiation factor 2BcomplexEukaryotic translation initiation factor 3core complexExocystExomer complexFAR complexGeneral transcription factor complex TFIIDGeneral transcription factor complex TFIIHGET4-GET5 transmembrane domain recognition complexGID ubiquitin ligase complexGin4 complexGolgi transport complexGSE complexHMC complexHOPS complexINO80 chromatin remodeling complexLSM1-7 complexLsm1-7-Pat1 complexLSM2-7 complexLSM2-8 complexMCM complexMICOS complexMitochondrial electron transport complex IIIMitochondrial inner membrane pre-sequence translocasecomplexMitochondrial outer membrane translocase holocomplexMitochondrial proton-transporting ATP synthase complexMitochondrial pyruvate dehydrogenase complexNineTeen complexNOP8 60s ribosome pre-assembly complexNuA3 histone acetyltransferase complexNuA4 histone acetyltransferase complexNuclear condensin complexNuclear/nucleolar exosome complex, DIS3-RRP6 variantNuclear pore complexNucleolar ribonuclease MRP complexNucleolar ribonuclease P complexOligosaccharyl transferase complex variant 1Oligosaccharyl transferase complex variant 2PAF1 complexPAS complexPhosphatidylinositol 3-kinase complex IPolarisomePrefoldin complexPRP19-associated complexRad17 RFC-like complexRetromer complexRPD3L histone deacetylase complexRPD3S histone deacetylase complexRQC complexRSC complex variant RSC1RSC complex variant RSC2SAGA complexSCF-Met30 ubiquitin ligase complexSCF-Ufo1 ubiquitin ligase complexSEA complexSeptin complexSET3C histone deacetylase complexSignal recognition particleSLIK (SAGA-like) complexSmall ribosomal subunit processome, variant1Small ribosomal subunit processome, variant2Small ribosomal subunit processome, variant3SMC5-SMC6 SUMO ligase complexSm complexSpliceosomal commitment complexSPOTS complexSWI/SNF chromatin remodelling complexSwr1 chromatin remodelling complexTHO complexTranscription factor TFIIIC complexTranslocon complexTRAPPIII protein complexTRAPPII protein complexTRAPPI protein complexTREX complexU1 snRNPU2 snRNPU4 snRNPU4/U6 snRNPU4/U6.U5 tri-snRNP complexU5 snRNPU6 snRNP complexUTP-A complexUTP-B complexUTP-C complex variant 1UTP-C complex variant 2UTP-C complex variant 3Vacuolar proton translocating ATPase complex,Golgi variantVacuolar proton translocating ATPase complex,vacuole variantCellular Components••••••••••••••••actinbud neckcell peripherycytoplasmendosomeERGolgimitochondrionmixednuclear peripherynucleolusnucleusperoxisomespindle polevacuolar membranevacuole
Standard name
Human Ortholog
Description Protein required for synthesis of diphthamide; required along with Dph1p, Kti11p, Jjj3p, and Dph5p; diphthamide is a modified histidine residue of translation elongation factor 2 (Eft1p or Eft2p); may act in a complex with Dph1p and Kti11p
Localization
Cell Percentages cytoplasm (100%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-4

Dph2

Dph2


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Dph2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available