Standard name
Human Ortholog
Description Enzyme of the 'de novo' purine nucleotide biosynthetic pathway; contains aminoimidazole ribotide synthetase and glycinamide ribotide synthetase activities
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0.05 0 0.05 0.06 0.06 0.07 0.07 – 0.09 0.15 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 1.0 0.99 1.0 0.98 0.95 0.94 0.9 0.84 0.86 – 0.86 0.82 0.98 0.99 0.99 0.99 1.0 0.99 0.98 0.97 0.98 0.85 0.92 0.87
Endoplasmic Reticulum 0 0 0 0 0 0 0.08 0.13 0.09 – 0.08 0.08 0 0 0 0 0.05 0.07 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 1 1 1 5 – 7 14 0 0 0 1 0 0 0 0 0 1 0 1
Bud 0 1 0 0 0 0 1 2 3 – 0 2 1 1 0 3 0 0 0 1 0 1 3 5
Bud Neck 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 1 23 7 28 26 36 44 35 – 42 92 5 3 2 14 10 12 0 0 0 0 0 0
Cytoplasm 161 270 449 369 557 441 587 549 432 – 383 488 129 268 269 765 526 444 157 277 442 84 214 219
Endoplasmic Reticulum 0 2 1 8 24 21 55 84 45 – 34 48 1 3 0 26 24 30 0 0 1 2 6 7
Endosome 0 0 0 0 0 0 0 0 0 – 0 1 0 0 0 0 0 0 0 0 0 0 0 1
Golgi 0 0 0 0 0 0 0 0 3 – 1 2 0 0 0 1 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 3 – 2 1 1 0 0 0 0 0 0 0 0 1 0 3
Nucleus 0 0 0 0 0 0 1 2 1 – 1 1 0 0 0 0 1 1 0 1 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 1
Nucleolus 0 0 0 0 0 0 0 0 0 – 0 0 0 0 1 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 1
SpindlePole 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 1 3 2 7 1 – 3 12 0 1 2 3 1 5 0 3 2 0 1 3
Unique Cell Count 161 274 450 375 584 470 650 652 500 443 598 132 271 272 769 528 449 160 286 453 99 234 251
Labelled Cell Count 161 274 473 384 610 492 683 689 528 473 661 137 276 274 813 562 492 160 286 453 99 234 251


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 103.4 108.5 76.3 77.4 64.2 53.9 47.6 45.1 41.2 – 37.0 36.7 115.6 124.1 110.4 152.9 156.5 154.4 89.9 93.8 97.8
Std Deviation (1e-4) 26.2 30.0 20.2 21.8 17.3 14.7 13.4 11.4 11.0 – 10.2 9.6 39.7 39.0 40.8 42.9 35.3 40.0 32.8 32.2 31.9
Intensity Change (Log2) – – – 0.02 -0.25 -0.5 -0.68 -0.76 -0.89 – -1.04 -1.06 0.6 0.7 0.53 1.0 1.04 1.02 0.24 0.3 0.36


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 – 0 3.3 0 0 0 0 0 0
Bud 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Cell Periphery -2.5 -0.2 0.3 0.3 1.1 1.2 – 2.5 5.3 -0.6 -2.8 -3.1 -3.2 -2.8 -1.9
Cytoplasm -2.1 -4.3 -5.1 -6.6 -8.7 -7.9 – -7.9 -9.5 -2.5 -1.5 -1.5 -0.8 -0.4 -1.6
Endoplasmic Reticulum 0 4.0 4.2 6.1 7.7 6.3 – 5.7 5.9 0 0 0 3.6 4.3 5.3
Endosome 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 – 0 3.0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 100.969 142.0612 130.1976 110.5906 80.5769 134.5292 128.179 161.862 147.5065 151.0678 133.3983 161.169 115.0842 163.8874 145.8841 144.6332 109.8632 114.5274
Actin 0.0032 0.0004 0.0029 0.0087 0.0218 0.0006 0.0006 0.0001 0.0003 0.0001 0.0007 0.0001 0.0107 0.0002 0.0006 0.0002 0.0216 0.0219
Bud 0.0009 0.0013 0.0002 0.0003 0.0012 0.0001 0.0019 0.0001 0.0001 0.001 0.0013 0.0003 0.0016 0 0.0001 0.0004 0.0076 0.0007
Bud Neck 0.0005 0.0003 0.0004 0.0004 0.0023 0.0007 0.0003 0.0001 0.0001 0.0001 0.0002 0.0005 0.0004 0.0001 0.0002 0.0002 0.0014 0.0017
Bud Periphery 0.0007 0.002 0.0002 0.0001 0.0012 0 0.0008 0 0.0001 0.0012 0.0004 0.0001 0.0011 0 0 0 0.0236 0.0004
Bud Site 0.0015 0.0003 0.0003 0.0002 0.0013 0 0.001 0 0.0001 0 0.0006 0 0.0016 0.0001 0.0001 0 0.0082 0.0005
Cell Periphery 0.0014 0.0026 0.0002 0.0004 0.0012 0.0001 0.0006 0.0009 0.0005 0.0002 0.0011 0.0001 0.0004 0.0005 0 0.0001 0.0021 0.0003
Cytoplasm 0.8749 0.9723 0.9309 0.8741 0.73 0.9859 0.9515 0.9941 0.9731 0.9859 0.8986 0.994 0.8742 0.9967 0.984 0.9893 0.8297 0.8847
Cytoplasmic Foci 0.0207 0.0029 0.015 0.0117 0.0184 0.0004 0.0029 0.0001 0.0072 0.0002 0.011 0.0006 0.0058 0.0001 0.0003 0.0005 0.0007 0.0026
Eisosomes 0.0007 0 0.0001 0.0005 0.0065 0 0.0001 0 0.0001 0 0.0001 0 0.0006 0.0001 0 0 0.0006 0
Endoplasmic Reticulum 0.0022 0.0006 0.0006 0.0012 0.0087 0.0004 0.0029 0.0004 0.0005 0.0003 0.0002 0.0003 0.0054 0.0001 0.001 0.0001 0.0019 0.0148
Endosome 0.0085 0.0002 0.0047 0.0101 0.0276 0.0002 0.0037 0 0.0004 0.0002 0.0025 0.0003 0.0085 0 0.0019 0.0002 0.0089 0.0131
Golgi 0.0035 0 0.0022 0.0136 0.0083 0 0.0007 0 0.0001 0 0.0028 0 0.0015 0 0.0001 0 0.0029 0.0013
Lipid Particles 0.0076 0.0003 0.0134 0.0179 0.0243 0 0.0017 0.0001 0.0022 0 0.0138 0 0.0014 0 0.0001 0 0.0019 0.0005
Mitochondria 0.0156 0.0004 0.0021 0.0214 0.0246 0.0001 0.0049 0.0001 0.0019 0.0002 0.0161 0.0001 0.0059 0 0.0002 0.0001 0.0356 0.0015
None 0.0087 0.0055 0.0026 0.0013 0.0348 0.002 0.0031 0.0004 0.0041 0.0002 0.0136 0.0003 0.0066 0.0001 0.0005 0.0002 0.0011 0.0011
Nuclear Periphery 0.0085 0.0011 0.0073 0.003 0.019 0.0015 0.0054 0.0005 0.0012 0.0012 0.0015 0.0004 0.0324 0.0006 0.0054 0.0018 0.0072 0.0253
Nucleolus 0.0136 0.0003 0.0004 0.0211 0.0092 0 0.0039 0.0004 0.0021 0 0.0066 0 0.0035 0 0.0001 0.0001 0.0031 0.0002
Nucleus 0.0129 0.0015 0.003 0.0034 0.0119 0.0064 0.0053 0.0009 0.0018 0.0023 0.0061 0.0012 0.0274 0.0009 0.0044 0.0041 0.0073 0.0208
Peroxisomes 0.0016 0 0.0059 0.0024 0.0067 0 0.0004 0 0.0008 0 0.0033 0 0.0005 0 0 0 0.0007 0.0001
Punctate Nuclear 0.001 0.0001 0.0023 0.001 0.0289 0.0004 0.0004 0 0.0007 0 0.0004 0 0.0024 0 0.0004 0.0001 0.0003 0.0015
Vacuole 0.0083 0.007 0.0032 0.004 0.0068 0.001 0.0058 0.0016 0.0018 0.0055 0.0108 0.0013 0.0047 0.0003 0.0004 0.0019 0.019 0.0045
Vacuole Periphery 0.0036 0.0008 0.0018 0.0034 0.0052 0.0002 0.0022 0.0001 0.0007 0.0013 0.008 0.0002 0.0034 0 0.0002 0.0007 0.0146 0.0023

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 294.974 233.3891 172.881 162.5438 164.7965 247.2016 198.7196 186.7933 176.7042 178.0573
Translational Efficiency 1.5556 1.5141 1.5703 1.4014 1.4064 1.694 1.4397 1.3277 1.3081 1.3138

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
2155 1808 575 1355 1255 1408 219 128 3410 3216 794 1483

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 5340.51 8006.91 6644.66 5090.00 6212.57 7002.61 4772.90 5175.17 5661.46 7567.22 6128.39 5097.35
Standard Deviation 1370.29 1919.91 1672.22 1885.76 1685.28 1816.74 1148.53 1386.71 1552.03 1940.50 1757.46 1848.16
Intensity Change Log 2 — 0.584268 0.315218 -0.069312 — 0.172703 -0.380324 -0.263584 — 0.377600 -0.017023 -0.170520

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000022 0.000053 0.000142 0.001231 0.000035 0.000044 0.000084 0.000154 0.000027 0.000049 0.000126 0.001138
Bud Neck 0.000084 0.000314 0.005140 0.005068 0.000255 0.000283 0.002239 0.002156 0.000146 0.000300 0.004340 0.004816
Bud Site 0.001149 0.001297 0.001868 0.024923 0.001359 0.000801 0.002687 0.007380 0.001226 0.001080 0.002094 0.023409
Cell Periphery 0.001431 0.003418 0.008371 0.006561 0.006097 0.002164 0.003439 0.005725 0.003148 0.002869 0.007011 0.006488
Cytoplasm 0.952743* 0.916173* 0.678510* 0.766884* 0.913602* 0.918450* 0.792200* 0.806655* 0.938338* 0.917170* 0.709868* 0.770317*
Cytoplasmic Foci 0.002168 0.005637 0.020186 0.011266 0.004545 0.004652 0.011818 0.008610 0.003043 0.005206 0.017878 0.011037
Eisosomes 0.000000 0.000001 0.000004 0.000011 0.000001 0.000001 0.000002 0.000003 0.000000 0.000001 0.000004 0.000010
Endoplasmic Reticulum 0.000056 0.000070 0.000048 0.000621 0.000074 0.000035 0.000028 0.000014 0.000062 0.000054 0.000042 0.000568
Endosome 0.000060 0.000117 0.000237 0.008114 0.000114 0.000084 0.000248 0.000137 0.000080 0.000102 0.000240 0.007426
Golgi 0.005679 0.005374 0.003488 0.011367 0.009418 0.003329 0.004624 0.000839 0.007055 0.004479 0.003801 0.010459
Lipid Particles 0.001264 0.002992 0.006370 0.008742 0.003938 0.001481 0.003836 0.010607 0.002248 0.002331 0.005671 0.008903
Mitochondria 0.000118 0.000136 0.000300 0.002523 0.000112 0.000134 0.000102 0.000052 0.000116 0.000135 0.000246 0.002309
Mitotic Spindle 0.000177 0.000812 0.000900 0.010388 0.000259 0.000468 0.001421 0.001323 0.000207 0.000661 0.001044 0.009605
None 0.004163 0.006767 0.004866 0.006692 0.004255 0.007910 0.003749 0.003520 0.004197 0.007267 0.004558 0.006418
Nuclear Periphery 0.000054 0.000202 0.001857 0.000385 0.000176 0.000135 0.000400 0.000196 0.000099 0.000173 0.001455 0.000369
Nuclear Periphery Foci 0.001590 0.003007 0.003673 0.003342 0.003678 0.001653 0.002713 0.003132 0.002359 0.002414 0.003408 0.003324
Nucleolus 0.000033 0.000060 0.000213 0.000532 0.000039 0.000100 0.000149 0.000128 0.000035 0.000077 0.000196 0.000497
Nucleus 0.014842 0.038892 0.192524* 0.048425 0.023691 0.044931 0.113960 0.057709 0.018099 0.041536 0.170855* 0.049226
Peroxisomes 0.000009 0.000019 0.000013 0.000401 0.000010 0.000005 0.000017 0.000097 0.000009 0.000013 0.000014 0.000375
Vacuole 0.006910 0.012173 0.069125 0.075778 0.021091 0.011123 0.054454 0.091004 0.012129 0.011713 0.065078 0.077092
Vacuole Periphery 0.007448 0.002488 0.002165 0.006749 0.007252 0.002217 0.001831 0.000558 0.007376 0.002370 0.002073 0.006215

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -3.21 -12.78 -5.58 -5.35 -4.34 -1.29 -3.72 -5.26 -5.03 -0.93 -3.60 -11.27 -5.67 -5.50 -4.39
Bud Neck -8.10 -11.80 -9.76 -9.22 5.00 -1.05 -6.15 -5.75 -5.66 0.11 -5.77 -13.06 -10.40 -9.97 4.44
Bud Site -1.01 -5.66 -9.48 -9.37 -8.46 2.86 -2.15 -5.61 -6.03 -0.83 0.41 -3.99 -9.78 -9.83 -7.72
Cell Periphery -5.27 -8.68 -8.86 -3.34 2.61 6.06 3.87 1.92 -2.30 -1.35 0.45 -5.39 -4.80 -5.13 1.38
Cytoplasm 11.05 35.13 28.86 22.05 -13.22 -0.30 12.20 10.14 10.30 0.35 8.44 34.99 28.72 24.80 -11.27
Cytoplasmic Foci -8.50 -12.67 -10.95 -6.61 6.83 -0.86 -6.08 -4.96 -4.72 0.29 -6.89 -13.83 -11.00 -7.95 6.70
Eisosomes -5.84 -13.66 -9.66 -9.04 -1.13 0.65 -5.96 -6.79 -6.87 -2.23 -4.53 -14.63 -10.04 -9.67 -2.18
Endoplasmic Reticulum -0.54 -1.31 -2.80 -2.63 -2.71 2.43 1.48 3.66 1.35 3.50 0.42 -0.06 -2.71 -2.75 -2.71
Endosome -3.04 -8.95 -5.79 -5.72 -5.41 0.88 -5.13 -2.52 -2.77 3.05 -1.80 -10.11 -5.78 -5.74 -5.37
Golgi 0.27 3.60 -1.41 -1.59 -3.26 7.01 5.34 10.43 7.74 4.43 4.61 6.55 0.06 -2.08 -2.96
Lipid Particles -4.20 -9.70 -10.99 -8.23 -0.93 4.28 -1.67 -3.65 -4.31 -3.18 -0.72 -9.08 -10.59 -10.38 -2.72
Mitochondria -0.36 -6.30 -3.50 -3.44 -2.88 -0.90 -1.80 2.43 3.46 5.15 -0.67 -6.83 -3.50 -3.43 -2.96
Mitotic Spindle -2.93 -4.79 -5.39 -4.88 -4.40 -1.80 -3.41 -1.46 -0.80 2.16 -3.47 -5.63 -5.42 -4.99 -3.97
None -4.57 0.72 -1.00 1.38 -1.37 -4.89 2.27 3.19 6.55 1.51 -6.57 1.49 -0.84 2.24 -1.41
Nuclear Periphery -6.85 -6.65 -8.94 -4.19 5.85 0.75 -6.94 -3.72 -3.92 3.46 -5.22 -7.02 -8.39 -5.47 6.06
Nuclear Periphery Foci -3.85 -6.78 -6.86 -1.78 1.78 4.03 0.45 -0.86 -3.51 -1.22 -0.64 -5.39 -4.89 -4.26 1.13
Nucleolus -2.23 -11.93 -4.43 -4.11 -1.62 -2.32 -5.59 -4.30 -1.05 2.23 -3.17 -13.10 -4.50 -3.97 -1.68
Nucleus -15.17 -27.82 -18.53 -7.68 19.89 -10.05 -11.55 -5.99 -3.00 5.12 -18.45 -29.16 -18.45 -7.77 19.93
Peroxisomes -1.61 -5.82 -3.54 -3.41 -3.33 2.18 -4.27 -3.05 -3.30 -1.70 -0.94 -7.24 -3.61 -3.56 -3.34
Vacuole -5.29 -15.73 -23.15 -20.95 3.24 4.03 -6.90 -8.69 -9.30 -3.11 -0.70 -16.79 -23.26 -23.08 2.05
Vacuole Periphery 5.12 5.56 2.32 -2.67 -3.13 4.77 5.58 7.03 4.49 3.72 6.90 7.59 3.16 -2.62 -3.18
Standard name
Human Ortholog
Description Enzyme of the 'de novo' purine nucleotide biosynthetic pathway; contains aminoimidazole ribotide synthetase and glycinamide ribotide synthetase activities
Localization
Cell Percentages cytoplasm (100%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-2

Ade5,7

Ade5,7


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Ade5,7-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available