TheCellVision

OST5

Standard name	OST5 ORF YGL226C-A Aliases dolichyl-diphosphooligosaccharide--protein glycotransferase subunit
Human Ortholog
Description	Zeta subunit of the oligosaccharyltransferase complex of the ER lumen; complex catalyzes asparagine-linked glycosylation of newly synthesized proteins

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Scale images

OST5

Micrographs

Sub-cellular Localization

LOC-score
Cell count

	ensLOC																		DeepLoc
Localization	WT1	WT2	WT3	RAP60	RAP140	RAP220	RAP300	RAP380	RAP460	RAP540	RAP620	RAP700	HU80	HU120	HU160	rpd3Δ_1	rpd3Δ_2	rpd3Δ_3	WT1	WT2	WT3	AF100	AF140	AF180
Cortical Patches	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
Bud	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
Bud Neck	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
Bud Site	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	–	–	–	–	–	–
Cell Periphery	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0.06	0	0.08	0	0	0	0	0	0
Cytoplasm	0.93	0.95	0.95	0.92	0.9	0.75	0.7	0.65	0.65	0.67	0.58	0.63	0.9	0.97	0.98	0.92	0.9	0.88	0.61	0.71	0.61	0.58	0.64	0.61
Endoplasmic Reticulum	0	0	0	0	0	0	0	0	0	0	0	0	0.1	0	0	0.05	0.08	0.07	0.15	0.07	0.14	0.09	0.06	0.07
Endosome	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
Golgi	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
Mitochondria	0	0	0	0	0.09	0.32	0.45	0.48	0.51	0.41	0.58	0.53	0	0	0	0	0	0	0.07	0.06	0.1	0.14	0.1	0.12
Nucleus	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
Nuclear Periphery	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
Nucleolus	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
Peroxisomes	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
SpindlePole	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
Vac/Vac Membrane	0	0	0	0	0	0	0	0	0	0.05	0	0	0	0	0	0	0	0.05	0	0	0	0.07	0.05	0.06

	ensLOC																		DeepLoc
Localization	WT1	WT2	WT3	RAP60	RAP140	RAP220	RAP300	RAP380	RAP460	RAP540	RAP620	RAP700	HU80	HU120	HU160	rpd3Δ_1	rpd3Δ_2	rpd3Δ_3	WT1	WT2	WT3	AF100	AF140	AF180
Cortical Patches	2	1	0	0	1	1	0	0	0	0	0	0	2	0	0	4	3	3	27	12	12	5	4	10
Bud	1	3	2	0	1	3	10	14	14	4	20	11	0	0	0	2	1	0	28	15	6	9	10	15
Bud Neck	6	3	0	0	0	0	0	0	0	0	0	0	0	2	0	0	1	3	10	9	4	5	5	5
Bud Site	0	0	0	0	0	0	0	1	6	2	2	1	0	0	0	0	0	0	–	–	–	–	–	–
Cell Periphery	5	2	4	5	2	1	2	0	4	3	3	3	6	3	7	19	7	22	16	7	10	4	4	4
Cytoplasm	806	442	372	275	384	350	415	402	370	307	388	350	445	386	594	314	202	248	562	354	246	206	209	228
Endoplasmic Reticulum	17	10	9	1	3	18	4	1	3	2	8	7	50	7	5	17	17	19	142	33	56	32	18	25
Endosome	6	0	2	2	8	2	2	2	4	10	1	2	1	1	1	0	2	1	15	7	6	2	6	4
Golgi	3	2	0	0	0	0	0	0	0	0	0	0	1	0	2	0	0	1	2	3	2	2	1	5
Mitochondria	9	4	13	13	38	152	263	294	289	187	393	299	1	0	0	6	5	6	62	31	41	50	32	44
Nucleus	1	0	0	1	3	2	4	8	0	1	5	3	1	1	1	2	1	1	0	0	0	0	0	0
Nuclear Periphery	0	1	0	1	1	1	3	3	0	1	1	0	0	0	0	0	0	0	2	0	1	0	3	0
Nucleolus	0	0	1	0	1	2	1	2	3	0	3	0	0	0	0	0	0	0	0	0	0	0	0	0
Peroxisomes	0	0	0	1	0	1	0	0	0	3	0	1	0	0	0	0	0	0	1	0	0	0	0	0
SpindlePole	0	1	2	3	0	2	6	2	6	4	3	9	0	0	1	0	0	0	13	5	1	2	3	3
Vac/Vac Membrane	27	5	3	11	16	6	13	11	21	23	11	10	14	6	8	8	8	14	20	13	5	23	16	20
Unique Cell Count	864	465	392	298	427	469	589	616	572	457	672	559	496	396	608	342	224	283	928	503	403	356	329	374
Labelled Cell Count	883	474	408	313	458	541	723	740	720	547	838	696	521	406	619	372	247	318	928	503	403	356	329	374

Yeast GFP Assignment

Endoplasmic Reticulum

(Huh et al. Nature 2003 425:686)

Protein Abundance

Screen	WT1	WT2	WT3	RAP60	RAP140	RAP220	RAP300	RAP380	RAP460	RAP540	RAP620	RAP700	HU80	HU120	HU160	rpd3Δ_1	rpd3Δ_2	rpd3Δ_3	AF100	AF140	AF180
Mean Cell GFP Intensity (1e^-4)	4.5	4.3	4.1	4.3	4.1	3.9	3.2	3.5	3.4	3.4	3.2	3.3	5.8	5.6	5.5	6.1	5.9	6.3	6.0	6.2	5.8
Std Deviation (1e^-4)	0.9	0.9	0.9	1.5	1.5	1.2	0.9	1.3	1.1	1.0	0.8	0.7	1.3	1.4	1.4	1.0	1.2	1.2	1.8	2.2	1.8
Intensity Change (Log₂)	–	–	–	0.08	0.02	-0.07	-0.34	-0.22	-0.27	-0.25	-0.33	-0.31	0.51	0.46	0.44	0.58	0.54	0.64	0.57	0.6	0.52

Localization Change

Localization	RAP60	RAP140	RAP220	RAP300	RAP380	RAP460	RAP540	RAP620	RAP700	HU80	HU120	HU160	rpd3Δ_1	rpd3Δ_2	rpd3Δ_3
Actin	–	–	–	–	–	–	–	–	–	–	–	–	–	–	–
Bud	–	–	–	–	–	–	–	–	–	–	–	–	0	–	0
Bud Neck	–	–	–	–	–	–	–	–	–	–	–	–	0	–	0
Bud Site	–	–	–	–	–	–	–	–	–	–	–	–	0	–	0
Cell Periphery	–	–	–	–	–	–	–	–	–	–	–	–	0	–	0
Cyto	–	–	–	–	–	–	–	–	–	–	–	–	–	–	–
Endoplasmic Reticulum	–	–	–	–	–	–	–	–	–	–	–	–	0	–	0
Endosome	–	–	–	–	–	–	–	–	–	–	–	–	0	–	0
Golgi	–	–	–	–	–	–	–	–	–	–	–	–	0	–	0
Mitochondria	–	–	–	–	–	–	–	–	–	–	–	–	0	–	0
Nuclear Periphery	–	–	–	–	–	–	–	–	–	–	–	–	0	–	0
Nuc	–	–	–	–	–	–	–	–	–	–	–	–	–	–	–
Nucleolus	–	–	–	–	–	–	–	–	–	–	–	–	0	–	0
Peroxisomes	–	–	–	–	–	–	–	–	–	–	–	–	0	–	0
SpindlePole	–	–	–	–	–	–	–	–	–	–	–	–	0	–	0
Vac	–	–	–	–	–	–	–	–	–	–	–	–	–	–	–
Cortical Patches	–	–	–	–	–	–	–	–	–	–	–	–	0	–	0
Cytoplasm	–	–	–	–	–	–	–	–	–	–	–	–	0	–	0
Nucleus	–	–	–	–	–	–	–	–	–	–	–	–	0	–	0
Vacuole	–	–	–	–	–	–	–	–	–	–	–	–	1.8	–	3.4

External localization resources

dHITS | Loqate | YeastGFP | YeastRGB | YeastRC

Images

Protein Concentration and Protein Localization Data

	R1						R2						R3
	G1 Pre-START	G1 Post-START	S/G2	Metaphase	Anaphase	Telophase	G1 Pre-START	G1 Post-START	S/G2	Metaphase	Anaphase	Telophase	G1 Pre-START	G1 Post-START	S/G2	Metaphase	Anaphase	Telophase
Concentration	0.1137	0.7678	0.5463	0.3929	0.324	0.7196	2.3179	3.2555	2.8978	3.1483	2.7004	3.1808	2.416	1.2832	1.3303	1.9245	2.6851	1.2405
Actin	0.0007	0.0038	0.0018	0.0035	0.0002	0.0021	0.0088	0.0005	0.0003	0.0019	0.0007	0.0009	0.0393	0.0005	0.0122	0.0272	0.002	0.0007
Bud	0.0018	0.0033	0.0015	0.0001	0	0.0041	0.0001	0.0003	0.0004	0.0003	0	0.0001	0.0011	0.0024	0.0001	0.0004	0.0018	0.0001
Bud Neck	0.0006	0.0016	0.0006	0.0004	0.0001	0.0042	0.0011	0.0002	0.0003	0.0009	0.0002	0.0011	0.0067	0.0001	0.0001	0.0012	0.0002	0.0005
Bud Periphery	0.0012	0.0011	0.0007	0.0002	0	0.0014	0.0001	0.0003	0.0005	0.0004	0	0.0002	0.0017	0.0024	0.0001	0.0007	0.0041	0.0001
Bud Site	0.002	0.0041	0.0062	0.0008	0.0001	0.0011	0.0006	0.0081	0.0021	0.0002	0	0.0006	0.0083	0.0051	0.0003	0.0017	0.0005	0.0002
Cell Periphery	0.0003	0.0004	0.0002	0.0004	0.0001	0.0002	0.0005	0.0006	0.0006	0.0012	0.0001	0.0002	0.0008	0.0005	0.0003	0.0002	0.0003	0.0002
Cytoplasm	0.1162	0.137	0.1321	0.1469	0.0574	0.1278	0.1897	0.2635	0.2756	0.4378	0.2734	0.2502	0.1286	0.3007	0.2931	0.2138	0.2429	0.3801
Cytoplasmic Foci	0.0124	0.0072	0.0046	0.0132	0.0022	0.0073	0.0092	0.0064	0.0073	0.0096	0.0578	0.0066	0.0402	0.0055	0.0206	0.0083	0.0023	0.006
Eisosomes	0.0002	0.0003	0.0001	0.0002	0.0001	0.0001	0.0015	0.0001	0.0001	0.0002	0.0001	0.0001	0.001	0.0001	0.0001	0.0007	0.0001	0.0001
Endoplasmic Reticulum	0.0092	0.0115	0.007	0.0202	0.0008	0.008	0.0271	0.0398	0.042	0.089	0.0522	0.0194	0.0351	0.0331	0.0306	0.0858	0.0092	0.0414
Endosome	0.0022	0.0031	0.0023	0.0053	0.0009	0.0011	0.0052	0.0057	0.0101	0.0183	0.0036	0.0044	0.0333	0.0038	0.0134	0.0313	0.0071	0.0056
Golgi	0.0002	0.0004	0.0003	0.0008	0	0.0001	0.0009	0.0003	0.0003	0.0006	0.0003	0.0002	0.0082	0.0003	0.0047	0.0101	0.0003	0.0006
Lipid Particles	0.0007	0.0007	0.0005	0.0021	0.0002	0.0003	0.0018	0.0007	0.0014	0.0027	0.0009	0.0012	0.0196	0.0008	0.0041	0.013	0.0003	0.0008
Mitochondria	0.0004	0.0009	0.0003	0.0006	0.0002	0.001	0.0003	0.0003	0.0004	0.0016	0.0004	0.0002	0.0228	0.0004	0.0016	0.0035	0.003	0.0004
None	0.8421	0.8086	0.8184	0.792	0.932	0.8232	0.7458	0.6627	0.6453	0.3987	0.5997	0.7074	0.5744	0.6362	0.5997	0.5428	0.5894	0.5477
Nuclear Periphery	0.0016	0.0021	0.0022	0.0021	0.0014	0.001	0.0018	0.0029	0.0034	0.016	0.0021	0.0021	0.0272	0.0023	0.006	0.0277	0.0513	0.0041
Nucleolus	0.0008	0.0012	0.001	0.0004	0.0003	0.0019	0.0002	0.0003	0.0005	0.0005	0.0001	0.0001	0.0042	0.0003	0.0002	0.0014	0.001	0.0003
Nucleus	0.0027	0.0062	0.0086	0.0027	0.002	0.0069	0.0016	0.003	0.0029	0.0092	0.0007	0.0016	0.0076	0.002	0.0013	0.0072	0.0627	0.0028
Peroxisomes	0.0001	0.0003	0.0002	0.0023	0.0001	0.0003	0.0003	0.0002	0.0001	0.0004	0.0006	0.0001	0.0113	0.0001	0.0019	0.006	0.0001	0.0003
Punctate Nuclear	0.0033	0.0048	0.0102	0.0047	0.0014	0.0068	0.0011	0.0012	0.0016	0.0042	0.0057	0.0017	0.0219	0.001	0.0071	0.0099	0.0024	0.0064
Vacuole	0.0012	0.0013	0.0008	0.0011	0.0005	0.0008	0.0021	0.0027	0.0045	0.0053	0.0013	0.0012	0.0045	0.0024	0.0018	0.0052	0.0103	0.0017
Vacuole Periphery	0.0001	0.0002	0.0001	0.0001	0.0001	0.0002	0.0002	0.0002	0.0003	0.0011	0.0002	0.0001	0.0023	0.0002	0.0005	0.0015	0.0087	0.0002

Sequencing Data

	R1					R2
	G1 Post-START	S/G2	Metaphase	Anaphase	Telophase	G1 Post-START	S/G2	Metaphase	Anaphase	Telophase
Gene Expression	114.1453	83.1428	64.4999	87.167	87.7004	54.9284	95.5291	73.5023	82.1201	99.3441
Translational Efficiency	1.521	1.8119	2.041	1.4077	1.7591	3.5951	1.8592	1.9649	1.7745	2.0556

Hit Data

Dataset	Hit
Protein Concentration	✔
Protein Localization	✘
Gene Expression	✘
Translational Efficiency	✘

Proteome Dynamics

Micrographs

Cell Count

R1				R2				R1 & R2
WT	UBP2	UBP14	UBP2UBP14	WT	UBP2	UBP14	UBP2UBP14	WT	UBP2	UBP14	UBP2UBP14
1070	1572	156	1910	1956	1532	1185	81	3026	3104	1341	1991

Protein Abundance

	R1				R2				R1 & R2
	WT	UBP2	UBP14	UBP2UBP14	WT	UBP2	UBP14	UBP2UBP14	WT	UBP2	UBP14	UBP2UBP14
Mean	665.27	722.01	860.15	796.65	685.12	702.15	824.92	858.93	678.10	712.21	829.02	799.18
Standard Deviation	80.56	99.88	87.23	107.95	81.78	99.85	104.10	113.26	81.90	100.36	102.90	108.87
Intensity Change Log ₂	—	0.118079	0.370648	0.260006	—	0.035423	0.267898	0.326184	—	0.076735	0.319432	0.293961

Localization Score (DeepLoc)

	R1				R2				R1 & R2
	WT	UBP2	UBP14	UBP2UBP14	WT	UBP2	UBP14	UBP2UBP14	WT	UBP2	UBP14	UBP2UBP14
Actin	0.000078	0.000369	0.000363	0.001359	0.000055	0.000597	0.000429	0.000178	0.000063	0.000481	0.000421	0.001311
Bud Neck	0.005787	0.016387	0.001658	0.009509	0.009810	0.027889	0.004890	0.000937	0.008387	0.022064	0.004514	0.009161
Bud Site	0.004273	0.014805	0.003929	0.026196	0.001967	0.012190	0.013128	0.001557	0.002783	0.013514	0.012058	0.025193
Cell Periphery	0.000365	0.000872	0.000419	0.000320	0.000138	0.000394	0.000331	0.000798	0.000218	0.000636	0.000341	0.000340
Cytoplasm	0.562874*	0.493397*	0.285062*	0.475971*	0.539836*	0.435172*	0.469345*	0.409331*	0.547982*	0.464660*	0.447907*	0.473260*
Cytoplasmic Foci	0.190739*	0.192507*	0.080217	0.027271	0.177304	0.236877*	0.034478	0.124757	0.182055*	0.214406*	0.039799	0.031237
Eisosomes	0.000136	0.000203	0.000176	0.000074	0.000146	0.000423	0.000084	0.000238	0.000142	0.000312	0.000094	0.000081
Endoplasmic Reticulum	0.000554	0.005434	0.025458	0.015040	0.002280	0.011050	0.039501	0.052876	0.001670	0.008206	0.037868	0.016579
Endosome	0.007511	0.012790	0.013308	0.004386	0.007690	0.022324	0.004218	0.014325	0.007627	0.017496	0.005275	0.004790
Golgi	0.000843	0.002103	0.000176	0.001272	0.000920	0.006094	0.000140	0.000712	0.000893	0.004073	0.000144	0.001249
Lipid Particles	0.007130	0.012342	0.014218	0.004122	0.009655	0.027019	0.006688	0.017933	0.008762	0.019586	0.007564	0.004684
Mitochondria	0.001161	0.003092	0.000283	0.001231	0.003640	0.008342	0.000365	0.000227	0.002763	0.005683	0.000356	0.001190
Mitotic Spindle	0.000568	0.002891	0.000108	0.010260	0.001853	0.002836	0.003213	0.000021	0.001398	0.002864	0.002852	0.009843
None	0.006374	0.005625	0.001822	0.003542	0.003903	0.002429	0.002084	0.003306	0.004777	0.004048	0.002053	0.003532
Nuclear Periphery	0.000373	0.000831	0.011904	0.004232	0.001000	0.001740	0.009371	0.004431	0.000778	0.001280	0.009665	0.004240
Nuclear Periphery Foci	0.000631	0.001503	0.033784	0.005539	0.000691	0.002299	0.015233	0.018953	0.000670	0.001896	0.017391	0.006084
Nucleolus	0.001058	0.001296	0.001492	0.000586	0.001109	0.001457	0.000369	0.001178	0.001091	0.001375	0.000500	0.000610
Nucleus	0.174855*	0.159353*	0.390962*	0.295565*	0.187955*	0.118183	0.319849*	0.242115*	0.183323*	0.139033*	0.328121*	0.293391*
Peroxisomes	0.001993	0.007188	0.000938	0.000564	0.002644	0.012565	0.000500	0.000878	0.002414	0.009842	0.000551	0.000577
Vacuole	0.031943	0.065887	0.133372	0.112617	0.046766	0.068835	0.075595	0.104964	0.041524	0.067342	0.082317	0.112306
Vacuole Periphery	0.000754	0.001124	0.000351	0.000344	0.000638	0.001283	0.000189	0.000287	0.000679	0.001203	0.000208	0.000342

Localization Changes (T-score)

	R1					R2					R1 & R2
	UBP2_WT	UBP14_WT	UBP2UBP14_WT	UBP2UBP14_UBP2	UBP2UBP14_UBP14	UBP2_WT	UBP14_WT	UBP2UBP14_WT	UBP2UBP14_UBP2	UBP2UBP14_UBP14	UBP2_WT	UBP14_WT	UBP2UBP14_WT	UBP2UBP14_UBP2	UBP2UBP14_UBP14
Actin	-4.17	-5.21	-2.63	-1.79	-1.72	-3.69	-1.98	-3.78	2.74	1.25	-5.20	-2.18	-2.69	-1.43	-1.52
Bud Neck	-7.03	5.66	-2.84	4.80	-8.55	-8.01	4.06	9.95	12.99	5.41	-9.89	4.05	-0.15	9.26	-3.75
Bud Site	-5.14	0.27	-7.33	-2.14	-6.06	-6.75	-4.15	0.80	7.08	4.32	-8.58	-3.81	-8.48	-2.82	-3.08
Cell Periphery	-2.65	-0.53	1.15	3.56	4.09	-3.59	-7.63	-4.88	-2.55	-3.41	-4.26	-3.03	-1.85	3.69	1.65
Cytoplasm	7.28	15.03	13.12	5.78	-8.69	11.66	8.86	5.29	1.47	2.12	12.75	13.33	14.63	3.42	-0.46
Cytoplasmic Foci	-0.16	11.54	29.52	33.69	6.26	-8.81	33.01	3.85	7.80	-6.70	-6.48	38.59	43.95	48.56	4.26
Eisosomes	-4.28	-3.59	9.41	8.63	8.25	-8.22	6.89	-3.06	3.93	-4.97	-9.07	6.46	9.76	12.57	2.89
Endoplasmic Reticulum	-5.18	-4.23	-9.52	-4.28	2.02	-5.41	-9.45	-2.92	-2.35	-0.76	-6.89	-10.24	-8.71	-3.22	6.10
Endosome	-4.92	-3.68	5.34	10.33	6.50	-9.03	6.54	-3.10	2.99	-4.85	-10.25	4.98	7.39	14.64	2.71
Golgi	-4.05	3.52	0.01	2.26	-1.35	-7.21	6.73	0.97	7.22	-1.99	-8.23	7.45	0.14	5.35	-1.46
Lipid Particles	-5.05	-3.77	5.43	10.50	6.14	-9.55	4.76	-2.77	3.10	-4.03	-10.34	2.61	9.58	15.65	6.11
Mitochondria	-3.34	2.17	0.28	4.47	-3.56	-3.35	4.18	4.37	7.04	3.23	-3.60	4.56	3.14	7.32	-3.14
Mitotic Spindle	-2.17	1.05	-6.29	-4.03	-6.64	-1.05	-1.59	2.46	3.91	3.38	-1.90	-1.92	-5.52	-4.36	-3.43
None	0.63	4.90	3.12	2.94	-2.48	1.96	2.96	0.51	-0.51	-0.85	1.07	5.31	2.27	1.42	-2.44
Nuclear Periphery	-5.61	-3.36	-12.14	-10.41	2.04	-2.79	-6.32	-1.75	-1.33	2.13	-3.42	-7.19	-10.68	-8.77	4.02
Nuclear Periphery Foci	-5.26	-4.25	-8.42	-6.47	3.59	-3.68	-9.16	-3.92	-3.53	-0.74	-5.23	-10.02	-9.08	-6.34	6.45
Nucleolus	-1.43	-1.46	3.68	5.49	3.87	-1.00	3.67	-0.18	0.64	-2.46	-1.37	4.10	3.75	5.10	-0.46
Nucleus	2.52	-9.55	-15.43	-18.05	4.53	13.79	-17.08	-2.72	-5.56	2.84	11.13	-20.15	-16.36	-23.76	5.59
Peroxisomes	-6.64	3.43	5.12	9.39	4.47	-8.74	6.98	5.75	10.68	-2.46	-10.74	7.87	8.90	14.40	0.36
Vacuole	-12.02	-9.21	-26.71	-18.76	-1.20	-7.42	-10.26	-4.73	-3.18	-1.58	-12.44	-14.03	-25.63	-19.77	-11.54
Vacuole Periphery	-0.96	1.44	1.69	4.35	1.44	-3.97	4.59	3.51	7.08	-2.03	-3.06	3.87	3.28	7.54	-1.41