Standard name | |
---|---|
Human Ortholog | |
Description | Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the mitochondrion; mtc3 is synthetically sick with cdc13-1 |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 3 | 5 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 4 | 7 | 1 | 0 | 0 | 0 | 1 | 4 | 7 | 6 | 24 | 37 |
Bud | 0 | 3 | 21 | 21 | 8 | 12 | 29 | 26 | 17 | 35 | 16 | 29 | 5 | 14 | 5 | 0 | 2 | 0 | 0 | 2 | 2 | 3 | 5 | 5 |
Bud Neck | 0 | 0 | 5 | 0 | 1 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 1 | 0 | 1 | 2 | 3 | 7 | 14 |
Bud Site | 0 | 0 | 3 | 2 | 1 | 4 | 7 | 7 | 9 | 10 | 3 | 10 | 2 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 3 | 10 | 4 | 3 | 3 | 2 | 2 | 0 | 3 | 3 | 0 | 1 | 22 | 15 | 14 | 14 | 14 | 18 | 0 | 0 | 1 | 0 | 2 | 0 |
Cytoplasm | 13 | 23 | 115 | 40 | 36 | 27 | 52 | 57 | 41 | 49 | 45 | 58 | 57 | 113 | 144 | 40 | 47 | 47 | 4 | 1 | 10 | 3 | 3 | 1 |
Endoplasmic Reticulum | 7 | 13 | 3 | 1 | 3 | 1 | 1 | 0 | 2 | 6 | 2 | 1 | 18 | 12 | 10 | 17 | 10 | 6 | 0 | 2 | 12 | 1 | 7 | 5 |
Endosome | 0 | 18 | 3 | 0 | 7 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 88 | 71 | 95 | 31 | 34 | 20 | 5 | 30 | 23 | 28 | 55 | 53 |
Golgi | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 14 | 11 | 2 | 1 | 1 | 4 | 0 | 7 | 3 | 21 | 36 | 25 |
Mitochondria | 87 | 141 | 221 | 224 | 267 | 257 | 440 | 375 | 421 | 462 | 326 | 421 | 262 | 197 | 174 | 9 | 15 | 14 | 80 | 193 | 275 | 287 | 452 | 377 |
Nucleus | 2 | 2 | 3 | 0 | 1 | 9 | 8 | 10 | 6 | 4 | 2 | 1 | 5 | 3 | 3 | 7 | 9 | 8 | 0 | 0 | 0 | 1 | 1 | 1 |
Nuclear Periphery | 0 | 2 | 0 | 0 | 1 | 0 | 8 | 2 | 6 | 2 | 0 | 3 | 2 | 0 | 1 | 0 | 0 | 0 | 1 | 1 | 3 | 2 | 5 | 3 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 3 | 3 | 3 | 5 | 1 | 1 | 2 | 1 | 1 | 1 | 0 | 1 | 1 | 1 | 0 | 0 | 1 | 2 | 2 |
Peroxisomes | 0 | 3 | 3 | 3 | 6 | 0 | 3 | 1 | 1 | 1 | 1 | 2 | 34 | 23 | 43 | 18 | 16 | 12 | 0 | 5 | 12 | 16 | 32 | 20 |
SpindlePole | 0 | 6 | 23 | 9 | 9 | 3 | 21 | 22 | 10 | 13 | 10 | 27 | 26 | 13 | 23 | 29 | 26 | 39 | 0 | 3 | 4 | 6 | 5 | 7 |
Vac/Vac Membrane | 9 | 23 | 9 | 7 | 21 | 12 | 13 | 20 | 12 | 12 | 3 | 8 | 87 | 57 | 79 | 41 | 33 | 22 | 11 | 12 | 14 | 19 | 29 | 26 |
Unique Cell Count | 100 | 208 | 344 | 247 | 313 | 276 | 483 | 432 | 448 | 499 | 354 | 458 | 515 | 457 | 516 | 167 | 163 | 151 | 113 | 275 | 384 | 411 | 677 | 588 |
Labelled Cell Count | 124 | 250 | 413 | 310 | 365 | 331 | 590 | 523 | 533 | 599 | 409 | 563 | 627 | 538 | 595 | 207 | 209 | 192 | 113 | 275 | 384 | 411 | 677 | 588 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 3.9 | 5.3 | 3.3 | 3.2 | 3.9 | 3.3 | 3.2 | 3.3 | 2.8 | 3.0 | 3.0 | 2.9 | 5.5 | 5.4 | 5.3 | 10.8 | 7.1 | 7.4 | 5.6 | 5.4 | 5.5 |
Std Deviation (1e-4) | 0.5 | 0.9 | 0.8 | 1.1 | 1.3 | 1.4 | 1.4 | 1.3 | 0.8 | 1.0 | 1.2 | 0.8 | 1.1 | 1.1 | 1.1 | 9.3 | 2.0 | 2.6 | 1.7 | 1.3 | 1.5 |
Intensity Change (Log2) | – | – | – | -0.04 | 0.24 | -0.03 | -0.07 | 0.01 | -0.27 | -0.17 | -0.14 | -0.21 | 0.72 | 0.71 | 0.67 | 1.7 | 1.1 | 1.15 | 0.76 | 0.71 | 0.73 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | -4.3 | -2.1 | -4.3 | -3.3 | -2.5 | -3.1 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2.6 | 2.0 | 1.6 | 4.2 | 4.2 | 5.3 |
Cytoplasm | -4.7 | -6.7 | -7.0 | -8.0 | -6.7 | -8.5 | -8.5 | -6.5 | -7.1 | -8.0 | -2.7 | -1.7 | -2.2 | -1.0 | -0.5 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2.4 | 1.8 | 1.3 | 5.1 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 7.6 | 7.1 | 7.9 | 7.5 | 8.1 | 6.0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3.1 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | -3.9 | -5.9 | -8.8 | -12.5 | -11.6 | -11.3 |
Nucleus | 0 | 0 | 2.1 | 0 | 1.6 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 4.1 | 3.3 | 4.8 | 5.3 | 5.0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | -1.0 | -2.6 | -1.4 | 3.7 | 3.3 | 5.9 |
Vacuole | 0.2 | 2.5 | 1.2 | 0.1 | 1.5 | 0.1 | -0.2 | -1.8 | -0.8 | 6.5 | 5.0 | 6.0 | 7.8 | 6.7 | 5.1 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 1.0836 | 1.7775 | 1.4008 | 1.1756 | 1.6672 | 1.4527 | 0.4828 | 0.5769 | 0.621 | 1.3328 | 0.6436 | 0.7128 | 1.8838 | 2.0028 | 1.9538 | 2.1469 | 1.0469 | 2.2768 |
Actin | 0.0127 | 0.0147 | 0.007 | 0.0021 | 0.0143 | 0.0131 | 0.0192 | 0.0077 | 0.0193 | 0.0503 | 0.0082 | 0.0138 | 0.0592 | 0.0182 | 0.0113 | 0.0637 | 0.1473 | 0.0291 |
Bud | 0.0008 | 0.0049 | 0.0036 | 0.0032 | 0.0097 | 0.0011 | 0.0032 | 0.0048 | 0.0041 | 0.0015 | 0.0212 | 0.0011 | 0.0018 | 0.0031 | 0.0085 | 0.0022 | 0.0058 | 0.0018 |
Bud Neck | 0.0076 | 0.0023 | 0.0087 | 0.0127 | 0.0013 | 0.0057 | 0.002 | 0.0015 | 0.008 | 0.0015 | 0.0023 | 0.005 | 0.0049 | 0.0018 | 0.0016 | 0.0009 | 0.003 | 0.0088 |
Bud Periphery | 0.0018 | 0.0075 | 0.0138 | 0.015 | 0.0239 | 0.0029 | 0.0065 | 0.0108 | 0.0099 | 0.004 | 0.0265 | 0.0042 | 0.0048 | 0.0062 | 0.008 | 0.0057 | 0.0168 | 0.0053 |
Bud Site | 0.0065 | 0.0236 | 0.0076 | 0.0038 | 0.0045 | 0.0021 | 0.0108 | 0.0329 | 0.031 | 0.0029 | 0.006 | 0.0014 | 0.0049 | 0.033 | 0.0159 | 0.0021 | 0.0078 | 0.002 |
Cell Periphery | 0.0014 | 0.0018 | 0.0029 | 0.0044 | 0.0005 | 0.0008 | 0.0019 | 0.0025 | 0.0022 | 0.0006 | 0.001 | 0.0008 | 0.0023 | 0.0019 | 0.0018 | 0.0008 | 0.0025 | 0.0016 |
Cytoplasm | 0.0128 | 0.0413 | 0.0646 | 0.036 | 0.0272 | 0.0289 | 0.0231 | 0.0578 | 0.0364 | 0.0055 | 0.0184 | 0.0236 | 0.023 | 0.0377 | 0.0412 | 0.0118 | 0.0198 | 0.0521 |
Cytoplasmic Foci | 0.0744 | 0.0984 | 0.0617 | 0.0245 | 0.0624 | 0.0691 | 0.0738 | 0.0965 | 0.0773 | 0.0829 | 0.0851 | 0.0586 | 0.0866 | 0.0943 | 0.1049 | 0.0316 | 0.0543 | 0.1026 |
Eisosomes | 0.0004 | 0.0008 | 0.0005 | 0.0002 | 0.0003 | 0.0003 | 0.0011 | 0.0005 | 0.0009 | 0.0003 | 0.0002 | 0.0004 | 0.0025 | 0.0012 | 0.0018 | 0.0007 | 0.0135 | 0.0009 |
Endoplasmic Reticulum | 0.0032 | 0.0054 | 0.0037 | 0.0026 | 0.0017 | 0.0034 | 0.005 | 0.0049 | 0.0025 | 0.0009 | 0.0033 | 0.0019 | 0.0171 | 0.0057 | 0.0023 | 0.0035 | 0.0023 | 0.0052 |
Endosome | 0.1828 | 0.1573 | 0.16 | 0.1668 | 0.1746 | 0.1427 | 0.1607 | 0.1315 | 0.123 | 0.1089 | 0.1195 | 0.0935 | 0.159 | 0.1784 | 0.1291 | 0.0521 | 0.0962 | 0.1489 |
Golgi | 0.075 | 0.0919 | 0.0776 | 0.0627 | 0.0576 | 0.0774 | 0.0534 | 0.0625 | 0.054 | 0.0902 | 0.0612 | 0.0531 | 0.0816 | 0.0908 | 0.0723 | 0.0278 | 0.0821 | 0.0963 |
Lipid Particles | 0.034 | 0.0334 | 0.0282 | 0.0044 | 0.0535 | 0.0363 | 0.0426 | 0.0141 | 0.0172 | 0.0388 | 0.0312 | 0.0205 | 0.0403 | 0.0324 | 0.023 | 0.0109 | 0.0095 | 0.0469 |
Mitochondria | 0.48 | 0.3869 | 0.4944 | 0.5727 | 0.5021 | 0.5346 | 0.4403 | 0.4723 | 0.4986 | 0.5492 | 0.4985 | 0.6119 | 0.3736 | 0.3877 | 0.4723 | 0.7043 | 0.4821 | 0.3904 |
None | 0.0361 | 0.0518 | 0.0173 | 0.0082 | 0.004 | 0.0236 | 0.0652 | 0.0275 | 0.044 | 0.0101 | 0.0282 | 0.0293 | 0.0669 | 0.0485 | 0.0273 | 0.0379 | 0.0293 | 0.041 |
Nuclear Periphery | 0.0024 | 0.0017 | 0.0026 | 0.0012 | 0.0094 | 0.0023 | 0.0088 | 0.0027 | 0.0012 | 0.0005 | 0.0105 | 0.005 | 0.0093 | 0.0013 | 0.0005 | 0.0016 | 0.0003 | 0.0018 |
Nucleolus | 0.0013 | 0.0018 | 0.0008 | 0.0005 | 0.002 | 0.001 | 0.004 | 0.0014 | 0.0016 | 0.0012 | 0.0023 | 0.0006 | 0.0007 | 0.0011 | 0.0005 | 0.0005 | 0.0004 | 0.0005 |
Nucleus | 0.0023 | 0.0019 | 0.0014 | 0.0013 | 0.0034 | 0.0019 | 0.0063 | 0.0031 | 0.0018 | 0.0008 | 0.0059 | 0.0013 | 0.0032 | 0.0015 | 0.0007 | 0.0008 | 0.0004 | 0.0008 |
Peroxisomes | 0.024 | 0.036 | 0.0207 | 0.0083 | 0.019 | 0.0334 | 0.0394 | 0.0435 | 0.0458 | 0.0375 | 0.0455 | 0.0634 | 0.0383 | 0.0334 | 0.0616 | 0.0274 | 0.0192 | 0.0429 |
Punctate Nuclear | 0.002 | 0.004 | 0.0008 | 0.0002 | 0.0126 | 0.0039 | 0.0063 | 0.0011 | 0.0007 | 0.0024 | 0.0073 | 0.0009 | 0.001 | 0.0054 | 0.0005 | 0.0004 | 0.0005 | 0.0007 |
Vacuole | 0.0263 | 0.0225 | 0.0137 | 0.0392 | 0.0088 | 0.0093 | 0.0188 | 0.0154 | 0.014 | 0.006 | 0.0109 | 0.006 | 0.0142 | 0.0113 | 0.01 | 0.0081 | 0.0039 | 0.0142 |
Vacuole Periphery | 0.012 | 0.0101 | 0.0084 | 0.0302 | 0.0072 | 0.006 | 0.0075 | 0.0051 | 0.0065 | 0.0042 | 0.0067 | 0.0037 | 0.0049 | 0.005 | 0.0051 | 0.005 | 0.003 | 0.0061 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 25.2122 | 10.6062 | 9.8378 | 14.923 | 24.9973 | 11.858 | 14.7454 | 16.2853 | 22.6688 | 17.5041 |
Translational Efficiency | 1.6937 | 3.2058 | 2.6192 | 2.1067 | 1.4611 | 4.9863 | 2.3244 | 1.5751 | 1.7841 | 2.0181 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✔ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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