Standard name
Description Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the mitochondrion; mtc3 is synthetically sick with cdc13-1

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06
Bud 0 0 0.06 0.09 0 0 0.06 0.06 0 0.07 0.05 0.06 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0.08 0.09 0.12 0 0 0 0 0 0
Cytoplasm 0.13 0.11 0.33 0.16 0.12 0.1 0.11 0.13 0.09 0.1 0.13 0.13 0.11 0.25 0.28 0.24 0.29 0.31 0 0 0 0 0 0
Endoplasmic Reticulum 0.07 0.06 0 0 0 0 0 0 0 0 0 0 0 0 0 0.1 0.06 0 0 0 0 0 0 0
Endosome 0 0.09 0 0 0 0 0 0 0 0 0 0 0.17 0.16 0.18 0.19 0.21 0.13 0.05 0.11 0.06 0.07 0.08 0.09
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.05 0
Mitochondria 0.87 0.68 0.64 0.91 0.85 0.93 0.91 0.87 0.94 0.93 0.92 0.92 0.51 0.43 0.34 0.05 0.09 0.09 0.72 0.71 0.72 0.7 0.67 0.64
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0.05 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0.05 0.08 0.11 0.1 0.08 0 0 0 0 0.05 0
SpindlePole 0 0 0.07 0 0 0 0 0.05 0 0 0 0.06 0.05 0 0 0.17 0.16 0.26 0 0 0 0 0 0
Vac/Vac Membrane 0.09 0.11 0 0 0.07 0 0 0.05 0 0 0 0 0.17 0.12 0.15 0.25 0.2 0.15 0.1 0.05 0 0.05 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 3 5 0 0 1 0 1 0 0 1 0 0 4 7 1 0 0 0 1 4 7 6 24 37
Bud 0 3 21 21 8 12 29 26 17 35 16 29 5 14 5 0 2 0 0 2 2 3 5 5
Bud Neck 0 0 5 0 1 0 2 0 0 0 0 0 0 1 0 0 1 1 0 1 2 3 7 14
Bud Site 0 0 3 2 1 4 7 7 9 10 3 10 2 0 0 0 0 0
Cell Periphery 3 10 4 3 3 2 2 0 3 3 0 1 22 15 14 14 14 18 0 0 1 0 2 0
Cytoplasm 13 23 115 40 36 27 52 57 41 49 45 58 57 113 144 40 47 47 4 1 10 3 3 1
Endoplasmic Reticulum 7 13 3 1 3 1 1 0 2 6 2 1 18 12 10 17 10 6 0 2 12 1 7 5
Endosome 0 18 3 0 7 1 0 0 0 0 0 0 88 71 95 31 34 20 5 30 23 28 55 53
Golgi 0 1 0 0 0 0 0 0 0 0 0 0 14 11 2 1 1 4 0 7 3 21 36 25
Mitochondria 87 141 221 224 267 257 440 375 421 462 326 421 262 197 174 9 15 14 80 193 275 287 452 377
Nucleus 2 2 3 0 1 9 8 10 6 4 2 1 5 3 3 7 9 8 0 0 0 1 1 1
Nuclear Periphery 0 2 0 0 1 0 8 2 6 2 0 3 2 0 1 0 0 0 1 1 3 2 5 3
Nucleolus 0 0 0 0 0 3 3 3 5 1 1 2 1 1 1 0 1 1 1 0 0 1 2 2
Peroxisomes 0 3 3 3 6 0 3 1 1 1 1 2 34 23 43 18 16 12 0 5 12 16 32 20
SpindlePole 0 6 23 9 9 3 21 22 10 13 10 27 26 13 23 29 26 39 0 3 4 6 5 7
Vac/Vac Membrane 9 23 9 7 21 12 13 20 12 12 3 8 87 57 79 41 33 22 11 12 14 19 29 26
Unique Cell Count 100 208 344 247 313 276 483 432 448 499 354 458 515 457 516 167 163 151 113 275 384 411 677 588
Labelled Cell Count 124 250 413 310 365 331 590 523 533 599 409 563 627 538 595 207 209 192 113 275 384 411 677 588


Mitochondria

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.9 5.3 3.3 3.2 3.9 3.3 3.2 3.3 2.8 3.0 3.0 2.9 5.5 5.4 5.3 10.8 7.1 7.4 5.6 5.4 5.5
Std Deviation (1e-4) 0.5 0.9 0.8 1.1 1.3 1.4 1.4 1.3 0.8 1.0 1.2 0.8 1.1 1.1 1.1 9.3 2.0 2.6 1.7 1.3 1.5
Intensity Change (Log2) -0.04 0.24 -0.03 -0.07 0.01 -0.27 -0.17 -0.14 -0.21 0.72 0.71 0.67 1.7 1.1 1.15 0.76 0.71 0.73


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 -4.3 -2.1 -4.3 -3.3 -2.5 -3.1
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 2.6 2.0 1.6 4.2 4.2 5.3
Cytoplasm -4.7 -6.7 -7.0 -8.0 -6.7 -8.5 -8.5 -6.5 -7.1 -8.0 -2.7 -1.7 -2.2 -1.0 -0.5
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 2.4 1.8 1.3 5.1 0 0
Endosome 0 0 0 0 0 0 0 0 0 7.6 7.1 7.9 7.5 8.1 6.0
Golgi 0 0 0 0 0 0 0 0 0 3.1 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 -3.9 -5.9 -8.8 -12.5 -11.6 -11.3
Nucleus 0 0 2.1 0 1.6 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 4.1 3.3 4.8 5.3 5.0 0
SpindlePole 0 0 0 0 0 0 0 0 0 -1.0 -2.6 -1.4 3.7 3.3 5.9
Vacuole 0.2 2.5 1.2 0.1 1.5 0.1 -0.2 -1.8 -0.8 6.5 5.0 6.0 7.8 6.7 5.1


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Mtc3-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available