Standard name | |
---|---|
Human Ortholog | |
Description | Mitochondrial component of the ERMES complex; links the ER to mitochondria and may promote inter-organellar calcium and phospholipid exchange as well as coordinating mitochondrial DNA replication and growth; required for mitophagy; ERMES complex is often co-localized with peroxisomes and with concentrated areas of pyruvate dehydrogenase |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 3 | 1 | 0 | 3 | 1 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 2 | 2 | 0 | 0 | 1 | 1 | 5 | 1 | 1 | 6 | 13 |
Bud | 22 | 11 | 6 | 22 | 20 | 16 | 35 | 78 | 35 | 36 | 42 | 70 | 24 | 38 | 34 | 20 | 4 | 3 | 5 | 3 | 0 | 2 | 4 | 7 |
Bud Neck | 4 | 2 | 0 | 0 | 4 | 2 | 6 | 13 | 4 | 5 | 4 | 3 | 0 | 2 | 4 | 3 | 1 | 1 | 3 | 2 | 0 | 1 | 0 | 6 |
Bud Site | 20 | 10 | 4 | 11 | 5 | 24 | 27 | 28 | 13 | 26 | 25 | 39 | 12 | 5 | 12 | 8 | 3 | 4 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 7 | 3 | 2 | 5 | 7 | 14 | 15 | 19 | 10 | 12 | 19 | 10 | 11 | 32 | 73 | 21 | 4 | 0 | 0 | 1 | 0 | 0 | 1 | 1 |
Endoplasmic Reticulum | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 3 | 2 | 0 | 2 | 1 | 0 | 0 | 0 | 1 | 2 |
Endosome | 30 | 25 | 2 | 9 | 19 | 6 | 3 | 6 | 1 | 3 | 5 | 5 | 99 | 172 | 228 | 79 | 25 | 16 | 87 | 51 | 25 | 53 | 82 | 111 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 2 | 0 | 10 | 8 | 1 | 5 | 4 | 12 |
Mitochondria | 120 | 85 | 40 | 60 | 47 | 54 | 81 | 92 | 68 | 103 | 87 | 156 | 55 | 45 | 41 | 22 | 7 | 3 | 9 | 9 | 6 | 8 | 9 | 20 |
Nucleus | 3 | 0 | 1 | 1 | 1 | 4 | 3 | 4 | 7 | 5 | 2 | 3 | 2 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 1 | 1 | 0 | 1 | 0 | 0 | 0 | 2 | 0 | 0 | 4 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 41 | 8 | 11 | 16 | 20 | 29 | 29 | 25 | 19 | 20 | 16 | 29 | 22 | 20 | 17 | 17 | 6 | 4 | 2 | 0 | 0 | 1 | 0 | 1 |
Peroxisomes | 129 | 86 | 12 | 49 | 84 | 41 | 52 | 45 | 18 | 36 | 29 | 60 | 235 | 342 | 323 | 110 | 56 | 33 | 102 | 63 | 39 | 109 | 152 | 220 |
SpindlePole | 197 | 61 | 42 | 100 | 155 | 168 | 242 | 337 | 127 | 180 | 138 | 295 | 264 | 381 | 403 | 153 | 85 | 65 | 156 | 50 | 34 | 126 | 165 | 125 |
Vac/Vac Membrane | 2 | 3 | 0 | 2 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 10 | 6 | 8 | 8 | 3 | 4 | 1 | 2 | 0 | 0 | 0 | 1 |
Unique Cell Count | 366 | 192 | 85 | 176 | 239 | 252 | 333 | 447 | 208 | 267 | 242 | 437 | 437 | 608 | 679 | 258 | 126 | 91 | 390 | 202 | 117 | 316 | 437 | 534 |
Labelled Cell Count | 575 | 299 | 122 | 275 | 367 | 359 | 493 | 648 | 306 | 426 | 367 | 674 | 734 | 1046 | 1149 | 445 | 197 | 136 | 390 | 202 | 117 | 316 | 437 | 534 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 4.7 | 5.3 | 4.5 | 4.6 | 4.6 | 3.9 | 3.2 | 3.6 | 3.5 | 2.9 | 3.3 | 2.8 | 6.0 | 5.4 | 4.7 | 6.8 | 7.1 | 7.7 | 5.5 | 5.1 | 5.8 |
Std Deviation (1e-4) | 0.7 | 0.9 | 0.8 | 1.5 | 1.3 | 1.4 | 1.1 | 1.0 | 1.6 | 1.1 | 1.7 | 1.2 | 1.6 | 1.5 | 1.5 | 2.2 | 2.0 | 2.2 | 1.3 | 1.2 | 1.8 |
Intensity Change (Log2) | – | – | – | 0.04 | 0.02 | -0.22 | -0.48 | -0.34 | -0.38 | -0.62 | -0.47 | -0.68 | 0.4 | 0.25 | 0.06 | 0.59 | 0.65 | 0.77 | 0.27 | 0.17 | 0.36 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud | – | – | – | – | – | – | – | 0 | – | 0 | -0.3 | 0 | 0.2 | 0 | 0 |
Bud Neck | – | – | – | – | – | – | – | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | – | – | – | – | – | – | – | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | – | – | – | – | – | – | – | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 |
Cyto | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endoplasmic Reticulum | – | – | – | – | – | – | – | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | – | – | – | – | – | – | – | 0 | – | 4.3 | 5.2 | 0 | 5.3 | 3.7 | 3.3 |
Golgi | – | – | – | – | – | – | – | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | – | – | – | – | – | – | – | 0 | – | -7.5 | -10.4 | 0 | -8.0 | -7.1 | -6.8 |
Nuclear Periphery | – | – | – | – | – | – | – | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 |
Nuc | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleolus | – | – | – | – | – | – | – | 0 | – | -2.7 | 0 | 0 | -1.9 | -2.1 | -2.0 |
Peroxisomes | – | – | – | – | – | – | – | 0 | – | 6.7 | 7.3 | 0 | 4.8 | 4.6 | 3.4 |
SpindlePole | – | – | – | – | – | – | – | 0 | – | 1.9 | 2.3 | 0 | 1.6 | 2.6 | 3.0 |
Vac | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cortical Patches | – | – | – | – | – | – | – | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | – | – | – | – | – | – | – | 1.8 | – | 0 | 0 | 2.5 | 1.8 | 0 | 0 |
Nucleus | – | – | – | – | – | – | – | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | – | – | – | – | – | – | – | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 3.2601 | 3.3116 | 3.0986 | 2.4052 | 2.7848 | 2.9926 | 1.6946 | 1.9461 | 1.7901 | 1.0873 | 1.6142 | 1.86 | 3.9296 | 4.2401 | 3.6315 | 4.1946 | 4.0041 | 3.9836 |
Actin | 0.0245 | 0.013 | 0.0144 | 0.0013 | 0.0035 | 0.0151 | 0.0388 | 0.0073 | 0.0112 | 0.0061 | 0.006 | 0.0081 | 0.0204 | 0.0053 | 0.0438 | 0.0086 | 0.0045 | 0.0134 |
Bud | 0.0005 | 0.0015 | 0.001 | 0.0001 | 0.0002 | 0.0004 | 0.0005 | 0.0003 | 0.0002 | 0.0002 | 0.0001 | 0.0003 | 0.0003 | 0.0008 | 0.0004 | 0.0001 | 0.0001 | 0.0002 |
Bud Neck | 0.0028 | 0.0007 | 0.0011 | 0.0002 | 0.0005 | 0.0096 | 0.0012 | 0.0009 | 0.0009 | 0.0006 | 0.0003 | 0.0006 | 0.0005 | 0.0004 | 0.0005 | 0.0106 | 0.0004 | 0.0044 |
Bud Periphery | 0.0004 | 0.001 | 0.0011 | 0 | 0.0002 | 0.0004 | 0.0006 | 0.0002 | 0.0002 | 0.0002 | 0.0001 | 0.0006 | 0.0003 | 0.0006 | 0.0003 | 0.0001 | 0 | 0.0002 |
Bud Site | 0.0032 | 0.0161 | 0.0133 | 0.0002 | 0.0004 | 0.0016 | 0.0048 | 0.002 | 0.0007 | 0.0024 | 0.0003 | 0.0017 | 0.0019 | 0.0117 | 0.0023 | 0.0005 | 0.0002 | 0.0005 |
Cell Periphery | 0.0001 | 0.0001 | 0.0005 | 0 | 0 | 0.0001 | 0.0001 | 0 | 0.0001 | 0.0001 | 0 | 0.0001 | 0.0001 | 0 | 0.0002 | 0 | 0 | 0 |
Cytoplasm | 0.0027 | 0.0071 | 0.003 | 0.0007 | 0.0012 | 0.0027 | 0.0047 | 0.0013 | 0.0015 | 0.0003 | 0.0006 | 0.0005 | 0.0138 | 0.0007 | 0.0073 | 0.0007 | 0.0003 | 0.0013 |
Cytoplasmic Foci | 0.249 | 0.2791 | 0.2583 | 0.2275 | 0.2259 | 0.2279 | 0.2937 | 0.2923 | 0.2832 | 0.2305 | 0.3233 | 0.254 | 0.3038 | 0.3784 | 0.3015 | 0.2567 | 0.1561 | 0.2507 |
Eisosomes | 0.0002 | 0 | 0.0002 | 0 | 0 | 0 | 0.0002 | 0 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0 | 0.0001 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0.0024 | 0.0008 | 0.0007 | 0 | 0.0002 | 0.0002 | 0.0012 | 0.0002 | 0.0028 | 0.0001 | 0.0002 | 0.0004 | 0.0027 | 0 | 0.0023 | 0 | 0.0001 | 0.0001 |
Endosome | 0.0784 | 0.0489 | 0.0533 | 0.0324 | 0.0941 | 0.0444 | 0.0585 | 0.0513 | 0.0499 | 0.0375 | 0.062 | 0.0191 | 0.0572 | 0.0238 | 0.0277 | 0.025 | 0.0437 | 0.033 |
Golgi | 0.0476 | 0.0332 | 0.0501 | 0.033 | 0.1199 | 0.0478 | 0.0397 | 0.028 | 0.0509 | 0.069 | 0.0534 | 0.0366 | 0.0577 | 0.0179 | 0.0416 | 0.0354 | 0.0343 | 0.0491 |
Lipid Particles | 0.1213 | 0.0634 | 0.0699 | 0.1059 | 0.0615 | 0.1282 | 0.1501 | 0.0525 | 0.0838 | 0.0776 | 0.0436 | 0.12 | 0.0961 | 0.0704 | 0.0596 | 0.0861 | 0.0248 | 0.0982 |
Mitochondria | 0.0099 | 0.0137 | 0.0245 | 0.0014 | 0.0627 | 0.0156 | 0.0172 | 0.0022 | 0.0341 | 0.0302 | 0.0647 | 0.0072 | 0.0202 | 0.0036 | 0.0191 | 0.002 | 0.0041 | 0.0109 |
None | 0.0042 | 0.0006 | 0.0044 | 0 | 0.0001 | 0.0002 | 0.0058 | 0.0002 | 0.0024 | 0.0001 | 0.0003 | 0.0005 | 0.0031 | 0.0004 | 0.0006 | 0.0001 | 0.0001 | 0.0002 |
Nuclear Periphery | 0.005 | 0.0009 | 0.004 | 0 | 0.0002 | 0.0021 | 0.0119 | 0.0002 | 0.011 | 0 | 0.0003 | 0.0004 | 0.0081 | 0 | 0.004 | 0 | 0 | 0.0001 |
Nucleolus | 0.0015 | 0.0008 | 0.0012 | 0 | 0.0001 | 0.0006 | 0.0008 | 0.001 | 0.0002 | 0.0006 | 0.0001 | 0.0004 | 0.0006 | 0.0006 | 0.0002 | 0 | 0 | 0.0001 |
Nucleus | 0.0019 | 0.0013 | 0.0011 | 0 | 0.0001 | 0.0018 | 0.0066 | 0.0011 | 0.0011 | 0.0001 | 0.0001 | 0.0004 | 0.0039 | 0.0001 | 0.0004 | 0 | 0 | 0.0001 |
Peroxisomes | 0.416 | 0.5011 | 0.4807 | 0.5958 | 0.3942 | 0.4797 | 0.3482 | 0.534 | 0.4583 | 0.4675 | 0.4375 | 0.5402 | 0.3713 | 0.4809 | 0.459 | 0.5724 | 0.7297 | 0.5283 |
Punctate Nuclear | 0.0247 | 0.007 | 0.0104 | 0.0013 | 0.0337 | 0.0189 | 0.0134 | 0.0239 | 0.006 | 0.0751 | 0.0056 | 0.0082 | 0.0362 | 0.0038 | 0.0277 | 0.0016 | 0.0007 | 0.0085 |
Vacuole | 0.0028 | 0.0075 | 0.005 | 0.0001 | 0.0007 | 0.0018 | 0.001 | 0.0009 | 0.0008 | 0.0011 | 0.0007 | 0.0005 | 0.0011 | 0.0005 | 0.001 | 0.0001 | 0.0005 | 0.0004 |
Vacuole Periphery | 0.0009 | 0.0022 | 0.0018 | 0.0001 | 0.0006 | 0.0009 | 0.0009 | 0.0002 | 0.0008 | 0.0004 | 0.0005 | 0.0003 | 0.0008 | 0.0001 | 0.0005 | 0.0001 | 0.0002 | 0.0002 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 21.5883 | 14.2953 | 6.895 | 20.4727 | 13.4768 | 20.7773 | 22.223 | 20.2426 | 22.406 | 22.5723 |
Translational Efficiency | 0.5859 | 0.8659 | 1.9788 | 0.7196 | 1.003 | 0.8886 | 0.6963 | 0.8901 | 0.7466 | 0.7551 |
Dataset | Hit |
---|---|
Protein Concentration | ✔ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
227 | 1130 | 2473 | 1345 | 1777 | 1122 | 492 | 316 | 2004 | 2252 | 2965 | 1661 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 747.48 | 740.73 | 1005.73 | 1069.01 | 781.86 | 811.73 | 1089.13 | 1095.66 | 777.97 | 776.10 | 1019.57 | 1074.08 |
Standard Deviation | 100.09 | 127.08 | 126.90 | 153.07 | 108.07 | 117.15 | 141.38 | 140.80 | 107.75 | 127.29 | 133.08 | 151.18 |
Intensity Change Log 2 | — | -0.013087 | 0.428136 | 0.516168 | — | 0.054090 | 0.478194 | 0.486818 | — | 0.021647 | 0.453945 | 0.501238 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.001067 | 0.002440 | 0.002868 | 0.021486 | 0.000670 | 0.001099 | 0.003956 | 0.026083 | 0.000715 | 0.001772 | 0.003049 | 0.022360 |
Bud Neck | 0.016717 | 0.004778 | 0.017784 | 0.007649 | 0.006080 | 0.003992 | 0.021049 | 0.009073 | 0.007285 | 0.004386 | 0.018326 | 0.007920 |
Bud Site | 0.030128 | 0.011687 | 0.049218 | 0.043600 | 0.009450 | 0.006997 | 0.042739 | 0.058573 | 0.011793 | 0.009350 | 0.048143 | 0.046449 |
Cell Periphery | 0.000260 | 0.000124 | 0.000118 | 0.000112 | 0.000122 | 0.000092 | 0.000169 | 0.000168 | 0.000138 | 0.000108 | 0.000127 | 0.000123 |
Cytoplasm | 0.011881 | 0.007370 | 0.016143 | 0.010786 | 0.005423 | 0.002909 | 0.013639 | 0.012564 | 0.006155 | 0.005147 | 0.015727 | 0.011124 |
Cytoplasmic Foci | 0.188280* | 0.130101 | 0.194853* | 0.175188 | 0.189897* | 0.135419 | 0.247013* | 0.186995* | 0.189714* | 0.132750 | 0.203508* | 0.177434 |
Eisosomes | 0.000265 | 0.000084 | 0.000052 | 0.000068 | 0.000112 | 0.000051 | 0.000079 | 0.000081 | 0.000130 | 0.000067 | 0.000056 | 0.000071 |
Endoplasmic Reticulum | 0.000764 | 0.000212 | 0.000327 | 0.000471 | 0.000405 | 0.000282 | 0.001030 | 0.000860 | 0.000446 | 0.000247 | 0.000444 | 0.000545 |
Endosome | 0.051043 | 0.091431 | 0.091957 | 0.067710 | 0.062450 | 0.146282 | 0.109743 | 0.094952 | 0.061158 | 0.118759 | 0.094908 | 0.072892 |
Golgi | 0.054646 | 0.077582 | 0.021763 | 0.043639 | 0.071167 | 0.076251 | 0.025039 | 0.042663 | 0.069295 | 0.076919 | 0.022307 | 0.043453 |
Lipid Particles | 0.035048 | 0.033608 | 0.009443 | 0.016320 | 0.044764 | 0.031146 | 0.025614 | 0.014407 | 0.043663 | 0.032382 | 0.012126 | 0.015956 |
Mitochondria | 0.014254 | 0.011855 | 0.008014 | 0.014638 | 0.003556 | 0.020157 | 0.006231 | 0.011094 | 0.004768 | 0.015991 | 0.007718 | 0.013964 |
Mitotic Spindle | 0.022382 | 0.023516 | 0.263709* | 0.223063* | 0.038352 | 0.031939 | 0.214037* | 0.183215* | 0.036543 | 0.027713 | 0.255467* | 0.215482* |
None | 0.000923 | 0.000289 | 0.000131 | 0.000067 | 0.000314 | 0.000158 | 0.000184 | 0.000089 | 0.000383 | 0.000224 | 0.000139 | 0.000071 |
Nuclear Periphery | 0.000096 | 0.000048 | 0.000401 | 0.000290 | 0.000119 | 0.000090 | 0.000709 | 0.000279 | 0.000116 | 0.000069 | 0.000452 | 0.000288 |
Nuclear Periphery Foci | 0.023183 | 0.005080 | 0.006079 | 0.001868 | 0.017039 | 0.005330 | 0.022620 | 0.002361 | 0.017735 | 0.005204 | 0.008824 | 0.001962 |
Nucleolus | 0.005345 | 0.001164 | 0.001985 | 0.000232 | 0.002977 | 0.000943 | 0.002853 | 0.000260 | 0.003245 | 0.001054 | 0.002129 | 0.000238 |
Nucleus | 0.007997 | 0.000478 | 0.001551 | 0.000268 | 0.001298 | 0.001009 | 0.004436 | 0.000596 | 0.002057 | 0.000742 | 0.002030 | 0.000330 |
Peroxisomes | 0.532066* | 0.595999* | 0.298079* | 0.361847* | 0.543461* | 0.531314* | 0.237953* | 0.339295* | 0.542170* | 0.563772* | 0.288102* | 0.357557* |
Vacuole | 0.003076 | 0.001834 | 0.014083 | 0.009082 | 0.002066 | 0.003258 | 0.019035 | 0.012491 | 0.002180 | 0.002544 | 0.014905 | 0.009731 |
Vacuole Periphery | 0.000580 | 0.000320 | 0.001441 | 0.001616 | 0.000279 | 0.001283 | 0.001872 | 0.003902 | 0.000313 | 0.000800 | 0.001513 | 0.002051 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -1.91 | -4.25 | -10.44 | -9.18 | -9.32 | -2.09 | -2.80 | -5.40 | -5.31 | -4.60 | -2.80 | -5.90 | -11.91 | -11.12 | -10.39 |
Bud Neck | 3.23 | -0.67 | 2.26 | -3.93 | 10.13 | 3.24 | -7.01 | -2.93 | -4.67 | 4.91 | 4.04 | -12.32 | -1.63 | -6.21 | 11.24 |
Bud Site | 3.11 | -3.82 | -2.75 | -10.74 | 1.57 | 1.68 | -9.09 | -7.27 | -7.54 | -2.29 | 1.72 | -19.61 | -13.39 | -14.12 | 0.22 |
Cell Periphery | 2.68 | 2.83 | 3.02 | 0.77 | 1.18 | 2.76 | -2.57 | -2.20 | -3.39 | -0.22 | 2.60 | 0.77 | 1.07 | -1.93 | 0.45 |
Cytoplasm | 1.77 | -2.14 | 0.22 | -2.60 | 4.38 | 3.19 | -3.41 | -3.62 | -4.72 | 0.05 | 1.23 | -9.20 | -5.49 | -6.40 | 3.70 |
Cytoplasmic Foci | 3.95 | -0.23 | 1.01 | -6.61 | 2.92 | 8.65 | -4.57 | 0.67 | -4.49 | 4.09 | 10.56 | -1.53 | 2.45 | -8.02 | 4.27 |
Eisosomes | 6.23 | 7.60 | 7.06 | 2.30 | -4.38 | 9.45 | 3.86 | 3.05 | -3.00 | 0.01 | 8.50 | 10.94 | 8.75 | -0.45 | -3.57 |
Endoplasmic Reticulum | 3.84 | 2.60 | 1.63 | -2.70 | -0.99 | 2.28 | -2.45 | -1.56 | -2.19 | 0.73 | 4.61 | -0.43 | -0.80 | -3.19 | -0.46 |
Endosome | -4.67 | -4.94 | -2.11 | 4.55 | 5.70 | -12.88 | -5.98 | -3.84 | 5.47 | 1.20 | -12.78 | -8.72 | -3.09 | 10.03 | 5.41 |
Golgi | -4.97 | 7.94 | 2.32 | 9.81 | -9.12 | -1.71 | 17.75 | 6.68 | 7.16 | -4.10 | -3.18 | 25.44 | 10.01 | 12.32 | -10.03 |
Lipid Particles | 0.24 | 4.28 | 3.12 | 5.03 | -4.06 | 3.91 | 4.89 | 9.76 | 4.63 | 2.80 | 3.88 | 13.57 | 11.13 | 6.61 | -2.37 |
Mitochondria | 0.39 | 1.17 | -0.08 | -0.94 | -2.84 | -7.08 | -0.98 | -2.19 | 2.17 | -1.26 | -7.13 | -2.67 | -4.45 | 0.99 | -3.09 |
Mitotic Spindle | 0.48 | -28.02* | -20.21 | -23.70* | 3.98 | 2.09 | -12.95 | -9.51 | -10.03 | 1.85 | 3.41 | -34.84 | -23.77 | -25.61 | 4.51 |
None | 1.30 | 2.30 | 2.42 | 1.91 | 4.73 | 2.18 | 2.04 | 2.75 | 0.25 | 0.99 | 0.82 | 3.63 | 4.27 | 2.05 | 3.94 |
Nuclear Periphery | 3.35 | -11.55 | -3.20 | -4.43 | 2.58 | 2.29 | -4.53 | -3.15 | -3.77 | 3.35 | 5.31 | -11.77 | -3.70 | -4.85 | 3.92 |
Nuclear Periphery Foci | 2.95 | 2.77 | 3.46 | 3.60 | 5.99 | 6.35 | -1.30 | 9.15 | 1.86 | 5.51 | 7.69 | 5.23 | 10.46 | 3.84 | 8.06 |
Nucleolus | 2.46 | 2.25 | 3.10 | 2.79 | 7.03 | 3.92 | 0.75 | 5.42 | 1.68 | 4.96 | 4.16 | 2.63 | 6.30 | 3.30 | 8.35 |
Nucleus | 2.17 | 1.75 | 2.20 | 0.59 | 4.66 | 0.54 | -2.68 | 0.64 | 0.02 | 2.85 | 2.36 | -0.72 | 3.09 | 0.70 | 5.29 |
Peroxisomes | -3.30 | 10.71 | 7.27 | 18.97 | -6.39 | 0.21 | 23.16 | 10.94 | 10.16 | -5.13 | -3.23 | 30.20 | 17.77 | 20.70 | -7.76 |
Vacuole | 2.00 | -10.67 | -6.10 | -7.50 | 4.37 | -1.81 | -7.07 | -4.88 | -4.27 | 2.30 | -0.81 | -13.69 | -8.65 | -8.08 | 4.87 |
Vacuole Periphery | 1.06 | -3.19 | -3.02 | -4.82 | -0.52 | -3.74 | -3.22 | -1.90 | -1.34 | -1.04 | -3.37 | -7.30 | -4.13 | -2.82 | -1.17 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
---|
Gene | Images |
---|