Standard name | |
---|---|
Human Ortholog | |
Description | Subunit of the heterodimeric FACT complex (Spt16p-Pob3p); FACT associates with chromatin via interaction with Nhp6Ap and Nhp6Bp, and reorganizes nucleosomes to facilitate access to DNA by RNA and DNA polymerases; specifically required for diauxic shift-induced H2B deposition onto rDNA genes; mutations cause reduced nucleosome occupancy over highly transcribed regions; coregulates transcription with Mot1p through preinitiation complex assembly and nucleosome organization |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | – | 0 | 0 | 0 | 1 | 2 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 2 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 1 | 2 | 6 | 2 | – | 3 | 8 | 0 | 1 | 6 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 1 | 2 | 1 | 3 | 11 | 3 | 11 | 7 | 2 | – | 4 | 3 | 8 | 23 | 29 | 12 | 9 | 23 | 0 | 0 | 0 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 2 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 1 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 2 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 1 |
Mitochondria | 6 | 1 | 3 | 1 | 2 | 11 | 17 | 25 | 26 | – | 19 | 17 | 2 | 1 | 11 | 5 | 11 | 36 | 0 | 0 | 0 | 0 | 0 | 3 |
Nucleus | 232 | 178 | 114 | 184 | 365 | 243 | 385 | 472 | 251 | – | 115 | 256 | 224 | 375 | 420 | 175 | 141 | 236 | 214 | 175 | 106 | 131 | 240 | 288 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | – | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 102 | 57 | 60 | 36 | 62 | 94 | 173 | 233 | 151 | – | 95 | 180 | 63 | 82 | 152 | 23 | 18 | 34 | 32 | 9 | 6 | 10 | 33 | 115 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 1 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 6 | 8 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vac/Vac Membrane | 0 | 3 | 0 | 0 | 1 | 0 | 4 | 1 | 0 | – | 0 | 1 | 2 | 2 | 5 | 3 | 5 | 4 | 3 | 4 | 5 | 6 | 30 | 102 |
Unique Cell Count | 252 | 189 | 121 | 191 | 397 | 277 | 473 | 591 | 313 | 166 | 324 | 239 | 418 | 506 | 201 | 167 | 285 | 253 | 192 | 119 | 152 | 306 | 521 | |
Labelled Cell Count | 341 | 242 | 178 | 224 | 442 | 353 | 592 | 747 | 432 | 236 | 466 | 299 | 493 | 635 | 222 | 188 | 334 | 253 | 192 | 119 | 152 | 306 | 521 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 31.6 | 29.2 | 19.2 | 19.6 | 22.6 | 19.1 | 19.4 | 18.0 | 16.3 | – | 15.4 | 16.2 | 32.0 | 29.8 | 26.0 | 36.2 | 37.2 | 38.6 | 28.3 | 28.1 | 29.7 |
Std Deviation (1e-4) | 9.3 | 8.3 | 5.4 | 6.2 | 8.4 | 6.8 | 6.4 | 7.0 | 5.4 | – | 5.4 | 6.0 | 10.4 | 11.2 | 10.0 | 16.5 | 20.7 | 17.2 | 9.0 | 7.5 | 8.4 |
Intensity Change (Log2) | – | – | – | 0.03 | 0.23 | -0.01 | 0.01 | -0.1 | -0.24 | – | -0.32 | -0.25 | 0.73 | 0.63 | 0.43 | 0.91 | 0.95 | 1.0 | 0.56 | 0.55 | 0.63 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 2.2 | 2.3 | 0 | 0 | 2.8 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 0 | 0 | 0 | 2.2 | – | 2.8 | 1.3 | 0 | 0 | 0 | 0 | 1.6 | 3.2 |
Nucleus | 0.9 | -0.8 | -2.0 | -3.4 | -3.8 | -3.6 | – | -5.2 | -3.8 | -0.2 | -1.5 | -3.1 | -2.0 | -2.6 | -3.0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | -5.7 | -7.7 | -2.9 | -2.6 | -2.1 | -0.3 | – | 1.3 | 1.1 | -4.4 | -6.6 | -4.1 | -7.6 | -7.3 | -8.2 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 22.6244 | 27.7235 | 25.3686 | 18.8449 | 23.2352 | 24.5535 | 28.8895 | 33.6569 | 31.6364 | 29.6054 | 23.6357 | 31.4879 | 24.7998 | 26.495 | 24.3368 | 22.2767 | 19.5577 | 22.5696 |
Actin | 0.0719 | 0 | 0.0375 | 0.0461 | 0 | 0 | 0.0299 | 0 | 0.0016 | 0.0222 | 0.0095 | 0.0043 | 0.0017 | 0 | 0.0051 | 0.0037 | 0.0013 | 0.0028 |
Bud | 0.0004 | 0 | 0.0002 | 0.0017 | 0.0001 | 0.0001 | 0.0004 | 0 | 0.0005 | 0.0006 | 0.0006 | 0.0002 | 0.0001 | 0 | 0.0001 | 0.0001 | 0.0001 | 0.0001 |
Bud Neck | 0.0003 | 0 | 0.0003 | 0.0007 | 0.0001 | 0.0001 | 0.0002 | 0 | 0.0002 | 0.0001 | 0.0003 | 0.0003 | 0.0003 | 0 | 0.0015 | 0.0001 | 0.0001 | 0.0001 |
Bud Periphery | 0.0006 | 0 | 0.0002 | 0.0033 | 0.0003 | 0.0001 | 0.0004 | 0 | 0.0008 | 0.0007 | 0.0008 | 0.0005 | 0.0001 | 0 | 0.0002 | 0.0003 | 0.0001 | 0.0004 |
Bud Site | 0.0024 | 0 | 0.0009 | 0.0017 | 0.0001 | 0 | 0.0008 | 0 | 0.0009 | 0.0009 | 0.0017 | 0.0001 | 0.0033 | 0 | 0.0041 | 0.0007 | 0.0001 | 0.0001 |
Cell Periphery | 0.0002 | 0 | 0.0001 | 0.0004 | 0.0004 | 0.0002 | 0.0001 | 0 | 0.0004 | 0.0001 | 0.0004 | 0.0002 | 0.0001 | 0 | 0.0002 | 0.0001 | 0.0002 | 0.0001 |
Cytoplasm | 0.0048 | 0 | 0.0002 | 0.0024 | 0 | 0 | 0.0057 | 0 | 0.0011 | 0.0017 | 0.0218 | 0.0001 | 0.0003 | 0 | 0 | 0 | 0 | 0 |
Cytoplasmic Foci | 0.0176 | 0 | 0.0006 | 0.0096 | 0 | 0 | 0.0077 | 0 | 0.0071 | 0.0038 | 0.0074 | 0.0025 | 0.0082 | 0 | 0.0039 | 0.0006 | 0.0005 | 0 |
Eisosomes | 0.0005 | 0 | 0.0003 | 0.0003 | 0 | 0 | 0.0001 | 0 | 0.0007 | 0.0001 | 0.0002 | 0 | 0 | 0 | 0.0004 | 0.0004 | 0.0001 | 0.0001 |
Endoplasmic Reticulum | 0.004 | 0 | 0.0005 | 0.0037 | 0 | 0 | 0.0015 | 0 | 0.0002 | 0.0007 | 0.0045 | 0.0003 | 0.0001 | 0 | 0 | 0 | 0 | 0.0002 |
Endosome | 0.0228 | 0 | 0.0029 | 0.065 | 0 | 0 | 0.0098 | 0 | 0.0011 | 0.003 | 0.0254 | 0.0015 | 0.0031 | 0 | 0.0001 | 0.0003 | 0.0004 | 0.0005 |
Golgi | 0.0108 | 0 | 0.0068 | 0.0545 | 0 | 0 | 0.002 | 0 | 0.0004 | 0.0012 | 0.0025 | 0.0013 | 0.0016 | 0 | 0.0002 | 0.0005 | 0.001 | 0.0005 |
Lipid Particles | 0.0137 | 0 | 0.0003 | 0.0054 | 0 | 0 | 0.0023 | 0 | 0.0031 | 0.0014 | 0.0022 | 0.0128 | 0.0079 | 0 | 0.0008 | 0.0013 | 0.0065 | 0.0002 |
Mitochondria | 0.0123 | 0.0008 | 0.003 | 0.1792 | 0.0021 | 0.0006 | 0.003 | 0.0013 | 0.0048 | 0.0107 | 0.1092 | 0.0017 | 0.0019 | 0.01 | 0.0037 | 0.0433 | 0.0238 | 0.0086 |
None | 0.0067 | 0 | 0.0001 | 0.0006 | 0 | 0 | 0.0069 | 0 | 0.0017 | 0.0018 | 0.0063 | 0.0001 | 0.0005 | 0 | 0.0001 | 0.0001 | 0.0001 | 0.0001 |
Nuclear Periphery | 0.0246 | 0.0007 | 0.001 | 0.0023 | 0 | 0.0001 | 0.0148 | 0.0002 | 0.0059 | 0.0154 | 0.041 | 0.0079 | 0.0004 | 0.0003 | 0.0001 | 0.0002 | 0.0002 | 0.0004 |
Nucleolus | 0.0353 | 0.0285 | 0.0289 | 0.0071 | 0.4129 | 0.1345 | 0.0689 | 0.0228 | 0.0393 | 0.0353 | 0.1452 | 0.0948 | 0.0313 | 0.0189 | 0.024 | 0.018 | 0.2593 | 0.1027 |
Nucleus | 0.751 | 0.969 | 0.9153 | 0.5938 | 0.5832 | 0.8639 | 0.8194 | 0.9752 | 0.9201 | 0.8748 | 0.589 | 0.8681 | 0.9235 | 0.9706 | 0.9544 | 0.9191 | 0.6912 | 0.8825 |
Peroxisomes | 0.0041 | 0 | 0.0001 | 0.0024 | 0 | 0 | 0.0017 | 0 | 0.0004 | 0.0055 | 0.0017 | 0.0006 | 0.0073 | 0 | 0.0005 | 0.0111 | 0.0139 | 0.0001 |
Punctate Nuclear | 0.0103 | 0 | 0.0001 | 0.0003 | 0 | 0 | 0.023 | 0 | 0.0062 | 0.0184 | 0.0229 | 0.0018 | 0.0075 | 0 | 0 | 0 | 0.0001 | 0 |
Vacuole | 0.0024 | 0.0001 | 0.0002 | 0.0091 | 0.0003 | 0.0003 | 0.0007 | 0.0001 | 0.0014 | 0.0004 | 0.003 | 0.0004 | 0.0002 | 0 | 0.0002 | 0.0001 | 0.0004 | 0.0001 |
Vacuole Periphery | 0.0033 | 0.0007 | 0.0005 | 0.0105 | 0.0004 | 0.0002 | 0.0009 | 0.0002 | 0.0019 | 0.0011 | 0.0046 | 0.0005 | 0.0002 | 0.0001 | 0.0004 | 0.0001 | 0.0006 | 0.0002 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 147.1561 | 93.3059 | 74.1825 | 104.1228 | 121.289 | 113.1889 | 79.3399 | 76.0191 | 92.1376 | 136.6149 |
Translational Efficiency | 1.6481 | 1.5668 | 1.5103 | 1.4322 | 1.56 | 1.8935 | 1.336 | 1.169 | 1.1371 | 1.2742 |
Dataset | Hit |
---|---|
Protein Concentration | ✔ |
Protein Localization | ✘ |
Gene Expression | ✔ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
1766 | 717 | 423 | 135 | 1319 | 1725 | 1519 | 176 | 3085 | 2442 | 1942 | 311 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 1413.45 | 2231.21 | 3150.93 | 3566.64 | 1719.18 | 1956.99 | 2566.79 | 3235.15 | 1544.17 | 2037.50 | 2694.03 | 3379.04 |
Standard Deviation | 337.32 | 551.45 | 589.87 | 1112.45 | 440.91 | 490.33 | 617.96 | 880.71 | 413.68 | 524.13 | 657.74 | 1001.57 |
Intensity Change Log 2 | — | 0.658605 | 1.156557 | 1.335345 | — | 0.186916 | 0.578245 | 0.912112 | — | 0.418956 | 0.868066 | 1.118540 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.000428 | 0.000443 | 0.000649 | 0.000572 | 0.000416 | 0.000478 | 0.000535 | 0.000542 | 0.000423 | 0.000468 | 0.000560 | 0.000555 |
Bud Neck | 0.001607 | 0.003757 | 0.004404 | 0.004457 | 0.001422 | 0.002805 | 0.003721 | 0.003806 | 0.001528 | 0.003084 | 0.003870 | 0.004088 |
Bud Site | 0.000274 | 0.001037 | 0.000593 | 0.000878 | 0.000192 | 0.000452 | 0.000383 | 0.000925 | 0.000239 | 0.000624 | 0.000429 | 0.000905 |
Cell Periphery | 0.000088 | 0.000285 | 0.000019 | 0.000094 | 0.000090 | 0.000183 | 0.000072 | 0.000048 | 0.000089 | 0.000213 | 0.000061 | 0.000068 |
Cytoplasm | 0.000500 | 0.000312 | 0.000017 | 0.000609 | 0.000038 | 0.000085 | 0.000062 | 0.000152 | 0.000303 | 0.000152 | 0.000052 | 0.000350 |
Cytoplasmic Foci | 0.000096 | 0.000917 | 0.000731 | 0.000786 | 0.000151 | 0.000473 | 0.000561 | 0.002068 | 0.000119 | 0.000604 | 0.000598 | 0.001512 |
Eisosomes | 0.000180 | 0.000170 | 0.000290 | 0.000083 | 0.000434 | 0.000364 | 0.000242 | 0.000118 | 0.000288 | 0.000307 | 0.000252 | 0.000103 |
Endoplasmic Reticulum | 0.000338 | 0.000161 | 0.000162 | 0.000117 | 0.000224 | 0.000356 | 0.000164 | 0.000144 | 0.000289 | 0.000299 | 0.000164 | 0.000132 |
Endosome | 0.000010 | 0.000023 | 0.000002 | 0.000010 | 0.000009 | 0.000034 | 0.000003 | 0.001661 | 0.000010 | 0.000031 | 0.000003 | 0.000944 |
Golgi | 0.000013 | 0.000021 | 0.000027 | 0.000035 | 0.000032 | 0.000030 | 0.000014 | 0.004559 | 0.000021 | 0.000027 | 0.000017 | 0.002595 |
Lipid Particles | 0.000002 | 0.000008 | 0.000004 | 0.000003 | 0.000004 | 0.000050 | 0.000001 | 0.000005 | 0.000003 | 0.000038 | 0.000002 | 0.000004 |
Mitochondria | 0.000699 | 0.000777 | 0.001360 | 0.001905 | 0.000575 | 0.001186 | 0.001086 | 0.002696 | 0.000646 | 0.001066 | 0.001146 | 0.002353 |
Mitotic Spindle | 0.000007 | 0.000019 | 0.000006 | 0.000061 | 0.000793 | 0.000993 | 0.000025 | 0.006023 | 0.000343 | 0.000707 | 0.000021 | 0.003435 |
None | 0.001927 | 0.001377 | 0.000386 | 0.001303 | 0.000682 | 0.000561 | 0.000653 | 0.001733 | 0.001395 | 0.000800 | 0.000595 | 0.001547 |
Nuclear Periphery | 0.000019 | 0.000015 | 0.000013 | 0.000077 | 0.000010 | 0.000035 | 0.000025 | 0.000106 | 0.000015 | 0.000029 | 0.000023 | 0.000093 |
Nuclear Periphery Foci | 0.000016 | 0.000021 | 0.000001 | 0.000004 | 0.000017 | 0.000059 | 0.000001 | 0.000105 | 0.000017 | 0.000048 | 0.000001 | 0.000061 |
Nucleolus | 0.029893 | 0.020460 | 0.020937 | 0.013917 | 0.021545 | 0.021178 | 0.013246 | 0.019370 | 0.026324 | 0.020967 | 0.014921 | 0.017003 |
Nucleus | 0.961944* | 0.966191* | 0.957732* | 0.971609* | 0.970193* | 0.968041* | 0.976239* | 0.941896* | 0.965471* | 0.967498* | 0.972208* | 0.954794* |
Peroxisomes | 0.001445 | 0.003614 | 0.012367 | 0.003116 | 0.002593 | 0.001964 | 0.002689 | 0.003974 | 0.001936 | 0.002448 | 0.004797 | 0.003601 |
Vacuole | 0.000322 | 0.000272 | 0.000205 | 0.000235 | 0.000310 | 0.000448 | 0.000179 | 0.005867 | 0.000317 | 0.000397 | 0.000185 | 0.003422 |
Vacuole Periphery | 0.000192 | 0.000120 | 0.000094 | 0.000130 | 0.000269 | 0.000225 | 0.000098 | 0.004202 | 0.000225 | 0.000194 | 0.000097 | 0.002434 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -0.65 | -8.18 | -2.95 | -2.16 | 0.78 | -2.31 | -5.13 | -1.94 | -0.99 | -0.24 | -2.38 | -8.86 | -3.23 | -2.04 | -0.29 |
Bud Neck | -2.57 | -9.13 | -4.07 | -1.05 | -0.64 | -5.69 | -15.68 | -1.85 | -0.80 | -0.16 | -5.13 | -16.76 | -3.40 | -1.45 | -0.63 |
Bud Site | -1.61 | -3.86 | -1.78 | -0.21 | -1.16 | -2.59 | -4.66 | -1.54 | -0.90 | -1.14 | -2.47 | -3.91 | -2.30 | -1.02 | -1.77 |
Cell Periphery | -1.01 | 3.17 | -0.43 | 0.86 | -1.68 | -1.24 | 0.46 | 1.12 | 1.94 | 1.02 | -1.62 | 1.20 | 0.38 | 1.74 | -0.55 |
Cytoplasm | 0.68 | 2.09 | -0.94 | -1.04 | -1.20 | -1.57 | -1.03 | -2.39 | -0.54 | -1.83 | 0.98 | 1.91 | -0.92 | -1.10 | -1.24 |
Cytoplasmic Foci | -1.09 | -8.88 | -1.61 | -0.20 | -0.73 | -1.66 | -1.77 | -1.30 | -0.93 | -1.00 | -1.94 | -2.74 | -1.84 | -0.99 | -1.24 |
Eisosomes | 0.18 | -6.27 | 5.99 | 2.73 | 11.88 | 0.75 | 2.25 | 3.50 | 5.12 | 3.99 | -0.43 | 0.91 | 4.48 | 6.15 | 7.85 |
Endoplasmic Reticulum | 6.33 | 8.09 | 7.66 | 1.34 | 1.82 | -3.13 | 2.59 | 1.77 | 3.96 | 0.38 | -0.45 | 7.53 | 5.62 | 4.56 | 1.04 |
Endosome | -0.93 | 1.97 | -0.35 | 0.74 | -1.78 | -1.42 | 0.95 | -1.46 | -1.43 | -1.46 | -1.64 | 1.95 | -1.46 | -1.41 | -1.47 |
Golgi | -0.88 | -5.00 | -2.49 | -1.46 | -1.55 | -0.09 | 0.87 | -1.21 | -1.21 | -1.22 | -0.84 | 0.47 | -1.22 | -1.21 | -1.22 |
Lipid Particles | -1.52 | -3.94 | -1.46 | 0.65 | -0.58 | -1.41 | 1.00 | -0.24 | 1.39 | -1.98 | -1.51 | 0.82 | -1.20 | 1.42 | -2.23 |
Mitochondria | -1.35 | -9.29 | -4.18 | -3.86 | -2.10 | -1.32 | -11.56 | -1.81 | -1.19 | -1.36 | -1.29 | -13.00 | -2.63 | -1.79 | -1.87 |
Mitotic Spindle | -1.15 | -0.07 | -1.55 | -1.27 | -1.55 | -0.27 | 1.02 | -0.87 | -0.82 | -1.01 | -0.76 | 1.00 | -0.92 | -0.78 | -1.02 |
None | 0.48 | 2.63 | 0.71 | 0.00 | -1.83 | 0.55 | 0.14 | -0.88 | -1.00 | -0.92 | 1.29 | 2.25 | -0.17 | -1.00 | -1.42 |
Nuclear Periphery | 0.36 | 1.57 | -2.42 | -2.47 | -2.59 | -3.26 | -5.41 | -2.26 | -1.59 | -1.87 | -2.56 | -2.86 | -3.13 | -2.51 | -2.84 |
Nuclear Periphery Foci | -0.40 | 2.55 | 0.64 | 0.69 | -1.42 | -1.57 | 1.37 | -1.12 | -0.42 | -1.37 | -1.70 | 2.52 | -1.06 | -0.14 | -1.47 |
Nucleolus | 3.41 | 3.47 | 3.43 | 1.27 | 1.52 | 0.13 | 4.11 | 0.36 | 0.31 | -1.04 | 3.01 | 7.34 | 2.31 | 0.94 | -0.59 |
Nucleus | -0.91 | 1.56 | -0.61 | -0.09 | -1.31 | 0.87 | -2.28 | 1.98 | 1.78 | 2.41 | -0.64 | -3.34 | 1.48 | 1.64 | 2.24 |
Peroxisomes | -4.28 | -9.67 | -1.55 | 0.16 | 5.37 | 1.27 | -0.22 | -0.83 | -1.31 | -0.79 | -1.75 | -7.55 | -1.72 | -1.22 | 1.16 |
Vacuole | 0.40 | 2.25 | 0.09 | -0.20 | -0.99 | -1.61 | 2.15 | -1.39 | -1.35 | -1.43 | -1.35 | 3.31 | -1.39 | -1.34 | -1.45 |
Vacuole Periphery | 2.73 | 4.91 | 1.29 | -0.70 | -1.70 | 0.73 | 3.18 | -1.30 | -1.32 | -1.36 | 0.97 | 4.99 | -1.29 | -1.31 | -1.37 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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