Standard name | |
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Human Ortholog | |
Description | Evolutionarily-conserved 5'-3' exonuclease; component of cytoplasmic processing (P) bodies involved in mRNA decay; enters the nucleus and positively regulates transcription initiation and elongation; involved in microtubule-mediated processes, filamentous growth, ribosomal RNA maturation, and telomere maintenance; negative regulator of autophagy; activated by the scavenger decapping enzyme Dcs1p; expression regulated by Ash1p in rich conditions |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 1 | 0 | 1 | 0 | 0 | 7 | 6 | 16 | 6 | 16 | 16 | 17 | 59 | 56 | 90 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 2 |
Bud | 0 | 0 | 2 | 0 | 0 | 1 | 2 | 4 | 4 | 3 | 1 | 5 | 16 | 30 | 47 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 3 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 4 | 6 | 3 | 3 | 8 | 8 | 19 | 20 | 11 | 27 | 14 | 24 | 1 | 4 | 8 | 6 | 11 | 8 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 204 | 300 | 145 | 148 | 240 | 221 | 268 | 254 | 106 | 152 | 109 | 157 | 182 | 214 | 219 | 168 | 144 | 138 | 190 | 300 | 140 | 176 | 307 | 325 |
Endoplasmic Reticulum | 4 | 12 | 2 | 2 | 9 | 23 | 54 | 62 | 29 | 40 | 29 | 41 | 23 | 21 | 23 | 10 | 5 | 2 | 9 | 5 | 10 | 9 | 32 | 79 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 10 | 6 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 6 | 0 | 2 | 1 | 1 | 114 | 117 | 101 | 1 | 0 | 4 | 0 | 0 | 0 | 0 | 1 | 7 |
Mitochondria | 1 | 0 | 0 | 3 | 2 | 10 | 29 | 42 | 20 | 56 | 54 | 63 | 0 | 4 | 10 | 2 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 2 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 2 | 0 | 0 | 0 | 1 | 1 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 2 | 5 | 10 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 6 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
Vac/Vac Membrane | 0 | 1 | 0 | 0 | 2 | 1 | 5 | 7 | 9 | 4 | 5 | 8 | 0 | 1 | 4 | 3 | 2 | 1 | 1 | 4 | 1 | 2 | 3 | 5 |
Unique Cell Count | 208 | 310 | 148 | 150 | 252 | 250 | 350 | 365 | 164 | 256 | 189 | 266 | 321 | 379 | 402 | 179 | 148 | 144 | 205 | 319 | 156 | 194 | 354 | 440 |
Labelled Cell Count | 214 | 319 | 154 | 156 | 261 | 272 | 384 | 413 | 186 | 302 | 229 | 318 | 400 | 464 | 525 | 191 | 162 | 155 | 205 | 319 | 156 | 194 | 354 | 440 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 32.9 | 23.5 | 16.7 | 17.4 | 18.9 | 18.1 | 16.7 | 15.8 | 14.8 | 14.2 | 12.9 | 12.9 | 27.8 | 27.1 | 23.9 | 25.9 | 30.4 | 32.3 | 25.5 | 26.2 | 27.7 |
Std Deviation (1e-4) | 6.1 | 4.7 | 3.0 | 3.7 | 3.7 | 4.5 | 3.5 | 3.5 | 4.1 | 3.5 | 3.6 | 3.5 | 4.8 | 5.3 | 5.3 | 7.5 | 8.2 | 7.7 | 4.8 | 5.7 | 5.4 |
Intensity Change (Log2) | – | – | – | 0.06 | 0.18 | 0.11 | -0.0 | -0.08 | -0.17 | -0.24 | -0.37 | -0.37 | 0.74 | 0.7 | 0.52 | 0.64 | 0.86 | 0.95 | 0.61 | 0.65 | 0.73 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 2.7 | 3.2 | 2.7 | 5.3 | 4.7 | 6.1 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1.9 | 2.8 | 3.8 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 1.7 | 1.7 | 2.0 | 3.1 | 2.2 | 2.8 | 0 | 0 | 0 | 0 | 2.2 | 1.6 |
Cytoplasm | 0.5 | -1.4 | -3.4 | -5.8 | -7.0 | -7.4 | -8.5 | -8.5 | -8.6 | -9.0 | -9.2 | -9.6 | -1.8 | -0.4 | -1.1 |
Endoplasmic Reticulum | 0 | 0 | 3.1 | 4.5 | 4.9 | 4.8 | 4.5 | 4.4 | 4.5 | 2.6 | 2.1 | 2.2 | 2.0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 8.3 | 7.7 | 6.7 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 0 | 3.6 | 4.3 | 4.4 | 6.1 | 7.1 | 6.4 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 24.7495 | 31.0582 | 28.9016 | 25.3548 | 30.6895 | 32.0015 | 27.2714 | 37.651 | 35.1508 | 34.3675 | 32.7221 | 35.1496 | 27.2455 | 33.9656 | 31.2648 | 28.4328 | 29.9878 | 31.1842 |
Actin | 0.0145 | 0.0016 | 0.0153 | 0.01 | 0.001 | 0.0061 | 0.0047 | 0.0006 | 0.0079 | 0.0102 | 0.0051 | 0.0026 | 0.0059 | 0.0016 | 0.0185 | 0.0061 | 0.002 | 0.0039 |
Bud | 0.0012 | 0.0009 | 0.0013 | 0.0016 | 0.0007 | 0.001 | 0.001 | 0.0006 | 0.0024 | 0.0012 | 0.0004 | 0.0004 | 0.0008 | 0.0004 | 0.0011 | 0.0045 | 0.0002 | 0.0004 |
Bud Neck | 0.001 | 0.0015 | 0.0017 | 0.0009 | 0.0018 | 0.0029 | 0.0006 | 0.0004 | 0.0016 | 0.002 | 0.0006 | 0.0018 | 0.0007 | 0.0003 | 0.0008 | 0.001 | 0.0007 | 0.003 |
Bud Periphery | 0.0016 | 0.0005 | 0.0011 | 0.0007 | 0.0002 | 0.0008 | 0.0008 | 0.0001 | 0.0047 | 0.0009 | 0.0005 | 0.0002 | 0.0008 | 0.0001 | 0.0005 | 0.0027 | 0 | 0.0002 |
Bud Site | 0.0028 | 0.02 | 0.0043 | 0.0004 | 0.0001 | 0.0002 | 0.001 | 0.0008 | 0.0017 | 0.0012 | 0.0003 | 0.0001 | 0.0014 | 0.0004 | 0.0006 | 0.0008 | 0.0001 | 0.0001 |
Cell Periphery | 0.0028 | 0.003 | 0.003 | 0.0007 | 0.0004 | 0.0003 | 0.0005 | 0.0003 | 0.0007 | 0.0013 | 0.0001 | 0.0002 | 0.0011 | 0.0006 | 0.0005 | 0.0008 | 0.0001 | 0.0002 |
Cytoplasm | 0.531 | 0.8367 | 0.7413 | 0.6883 | 0.848 | 0.7506 | 0.7992 | 0.9607 | 0.8963 | 0.8438 | 0.8344 | 0.8579 | 0.7548 | 0.8947 | 0.8367 | 0.8398 | 0.9543 | 0.7733 |
Cytoplasmic Foci | 0.007 | 0.0087 | 0.0078 | 0.0171 | 0.0042 | 0.0107 | 0.0156 | 0.0059 | 0.0118 | 0.0084 | 0.0143 | 0.0123 | 0.0102 | 0.0108 | 0.013 | 0.0077 | 0.0079 | 0.0158 |
Eisosomes | 0.0004 | 0 | 0.0001 | 0.0001 | 0 | 0 | 0 | 0 | 0.0002 | 0.0001 | 0.0001 | 0 | 0.0008 | 0 | 0.0001 | 0.0003 | 0 | 0 |
Endoplasmic Reticulum | 0.1624 | 0.0683 | 0.078 | 0.0055 | 0.0582 | 0.085 | 0.0517 | 0.0111 | 0.0199 | 0.0092 | 0.0112 | 0.0524 | 0.1255 | 0.0488 | 0.0378 | 0.0076 | 0.0115 | 0.1133 |
Endosome | 0.0223 | 0.0087 | 0.0269 | 0.017 | 0.0162 | 0.0621 | 0.0482 | 0.0103 | 0.0235 | 0.0396 | 0.0681 | 0.0477 | 0.0324 | 0.0232 | 0.0248 | 0.0348 | 0.0157 | 0.0462 |
Golgi | 0.0049 | 0.0095 | 0.0071 | 0.0037 | 0.0006 | 0.0055 | 0.0052 | 0.0011 | 0.004 | 0.0144 | 0.0056 | 0.0082 | 0.0063 | 0.0104 | 0.0106 | 0.0068 | 0.0012 | 0.0239 |
Lipid Particles | 0.0102 | 0.0056 | 0.0037 | 0.0248 | 0.0004 | 0.0004 | 0.0016 | 0.0002 | 0.0017 | 0.0019 | 0.0015 | 0.0002 | 0.0051 | 0.0004 | 0.0037 | 0.0076 | 0.0001 | 0.001 |
Mitochondria | 0.0291 | 0.0018 | 0.0024 | 0.0577 | 0.0008 | 0.0063 | 0.0042 | 0.0003 | 0.001 | 0.0067 | 0.0067 | 0.0038 | 0.0128 | 0.0024 | 0.0126 | 0.0277 | 0.0005 | 0.0057 |
None | 0.0015 | 0.0002 | 0.0017 | 0.0008 | 0.0022 | 0.0007 | 0.0042 | 0.0002 | 0.0007 | 0.0003 | 0.0006 | 0.0003 | 0.0042 | 0.0002 | 0.0029 | 0.0012 | 0.0003 | 0.0004 |
Nuclear Periphery | 0.055 | 0.0068 | 0.017 | 0.0103 | 0.0274 | 0.0363 | 0.0249 | 0.0013 | 0.0067 | 0.0045 | 0.014 | 0.003 | 0.0093 | 0.0013 | 0.0036 | 0.0036 | 0.0013 | 0.0034 |
Nucleolus | 0.0019 | 0.0001 | 0.0007 | 0.0002 | 0 | 0.0004 | 0.0003 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0 | 0.0012 | 0 | 0.0001 | 0.0003 | 0 | 0 |
Nucleus | 0.0149 | 0.0026 | 0.0051 | 0.0039 | 0.0042 | 0.0079 | 0.015 | 0.0009 | 0.0018 | 0.0021 | 0.0018 | 0.0012 | 0.0035 | 0.0005 | 0.0012 | 0.0017 | 0.0008 | 0.0011 |
Peroxisomes | 0.0013 | 0.0008 | 0.0018 | 0.0141 | 0.0001 | 0.0002 | 0.0004 | 0.0001 | 0.0002 | 0.0005 | 0.0002 | 0.0001 | 0.001 | 0.0001 | 0.0033 | 0.0005 | 0.0001 | 0.0001 |
Punctate Nuclear | 0.0133 | 0.0001 | 0.0015 | 0.0038 | 0.0001 | 0.0003 | 0.002 | 0.0001 | 0.0006 | 0.0002 | 0.0003 | 0.0001 | 0.0004 | 0.0001 | 0.0077 | 0.0002 | 0.0001 | 0.0001 |
Vacuole | 0.0859 | 0.0208 | 0.0649 | 0.1143 | 0.0246 | 0.0115 | 0.0149 | 0.0043 | 0.0104 | 0.0455 | 0.024 | 0.0051 | 0.0172 | 0.0028 | 0.0143 | 0.0322 | 0.0021 | 0.0051 |
Vacuole Periphery | 0.0348 | 0.002 | 0.0134 | 0.0242 | 0.0087 | 0.0107 | 0.004 | 0.0007 | 0.0021 | 0.006 | 0.0102 | 0.0025 | 0.0045 | 0.0009 | 0.0054 | 0.0121 | 0.0011 | 0.0026 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 165.2581 | 146.6555 | 152.0673 | 180.876 | 152.1994 | 143.5291 | 174.4284 | 182.1858 | 174.7641 | 190.0552 |
Translational Efficiency | 0.951 | 1.032 | 0.9902 | 0.8515 | 0.8743 | 1.0986 | 0.9466 | 0.7973 | 0.8636 | 0.838 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
460 | 981 | 802 | 949 | 2250 | 1950 | 1718 | 95 | 2710 | 2931 | 2520 | 1044 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 1473.55 | 2051.76 | 3842.60 | 2133.42 | 1830.36 | 1971.48 | 2593.92 | 2521.03 | 1769.79 | 1998.35 | 2991.32 | 2168.69 |
Standard Deviation | 322.75 | 397.81 | 590.13 | 434.07 | 313.66 | 376.35 | 410.17 | 454.79 | 342.50 | 385.54 | 750.88 | 450.02 |
Intensity Change Log 2 | — | 0.477566 | 1.382787 | 0.533872 | — | 0.107152 | 0.503007 | 0.461886 | — | 0.284183 | 0.962106 | 0.494436 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.000540 | 0.000959 | 0.000284 | 0.003713 | 0.000204 | 0.000325 | 0.001785 | 0.003601 | 0.000261 | 0.000537 | 0.001307 | 0.003702 |
Bud Neck | 0.000693 | 0.000873 | 0.004508 | 0.005022 | 0.000183 | 0.000399 | 0.002449 | 0.004810 | 0.000270 | 0.000558 | 0.003104 | 0.005002 |
Bud Site | 0.057742 | 0.031489 | 0.007685 | 0.063669 | 0.008630 | 0.014723 | 0.017980 | 0.074088 | 0.016967 | 0.020334 | 0.014703 | 0.064617 |
Cell Periphery | 0.015584 | 0.009219 | 0.010869 | 0.004898 | 0.003651 | 0.002672 | 0.008412 | 0.073872 | 0.005677 | 0.004863 | 0.009194 | 0.011174 |
Cytoplasm | 0.277992* | 0.526296* | 0.200327* | 0.308828* | 0.669562* | 0.648710* | 0.619112* | 0.341557* | 0.603096* | 0.607738* | 0.485832* | 0.311806* |
Cytoplasmic Foci | 0.243336* | 0.103564 | 0.022541 | 0.134621 | 0.127805 | 0.117853 | 0.029214 | 0.053618 | 0.147415 | 0.113070 | 0.027090 | 0.127250 |
Eisosomes | 0.000016 | 0.000013 | 0.000007 | 0.000018 | 0.000006 | 0.000006 | 0.000009 | 0.000039 | 0.000008 | 0.000008 | 0.000008 | 0.000020 |
Endoplasmic Reticulum | 0.047085 | 0.019727 | 0.003832 | 0.001870 | 0.022864 | 0.012437 | 0.004030 | 0.002196 | 0.026976 | 0.014877 | 0.003967 | 0.001900 |
Endosome | 0.022724 | 0.012476 | 0.005661 | 0.040728 | 0.005403 | 0.007888 | 0.007337 | 0.012705 | 0.008343 | 0.009424 | 0.006804 | 0.038178 |
Golgi | 0.151144* | 0.160703* | 0.150295* | 0.105456 | 0.069500 | 0.103237 | 0.073870 | 0.070386 | 0.083359 | 0.122471* | 0.098193 | 0.102265 |
Lipid Particles | 0.014452 | 0.034703 | 0.039325 | 0.051126 | 0.011724 | 0.009383 | 0.043276 | 0.173412* | 0.012187 | 0.017857 | 0.042019 | 0.062253 |
Mitochondria | 0.001555 | 0.000756 | 0.000162 | 0.001776 | 0.001061 | 0.000963 | 0.000167 | 0.000066 | 0.001145 | 0.000894 | 0.000166 | 0.001620 |
Mitotic Spindle | 0.003369 | 0.006088 | 0.010807 | 0.039079 | 0.001102 | 0.003847 | 0.009619 | 0.003478 | 0.001487 | 0.004597 | 0.009997 | 0.035839 |
None | 0.000456 | 0.000817 | 0.000814 | 0.000227 | 0.000875 | 0.000630 | 0.000622 | 0.000319 | 0.000804 | 0.000693 | 0.000683 | 0.000236 |
Nuclear Periphery | 0.000150 | 0.000171 | 0.003484 | 0.000335 | 0.000107 | 0.000122 | 0.000463 | 0.000262 | 0.000114 | 0.000138 | 0.001424 | 0.000329 |
Nuclear Periphery Foci | 0.005150 | 0.016846 | 0.055626 | 0.011576 | 0.006484 | 0.007407 | 0.024160 | 0.009620 | 0.006258 | 0.010567 | 0.034174 | 0.011398 |
Nucleolus | 0.000384 | 0.000305 | 0.000096 | 0.000118 | 0.000031 | 0.000030 | 0.000063 | 0.000073 | 0.000091 | 0.000122 | 0.000073 | 0.000113 |
Nucleus | 0.001110 | 0.001518 | 0.002971 | 0.001947 | 0.001074 | 0.000851 | 0.003640 | 0.002838 | 0.001080 | 0.001074 | 0.003427 | 0.002028 |
Peroxisomes | 0.000802 | 0.000343 | 0.000028 | 0.002260 | 0.000244 | 0.000167 | 0.000162 | 0.000914 | 0.000339 | 0.000226 | 0.000119 | 0.002137 |
Vacuole | 0.043672 | 0.034851 | 0.348329* | 0.192602 | 0.022068 | 0.026771 | 0.123454 | 0.157484 | 0.025736 | 0.029475 | 0.195021 | 0.189406 |
Vacuole Periphery | 0.112044* | 0.038283 | 0.132349* | 0.030133 | 0.047420 | 0.041579 | 0.030176 | 0.014662 | 0.058389 | 0.040476 | 0.062693 | 0.028726 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -2.46 | 2.61 | -6.40 | -5.32 | -6.85 | -1.43 | -2.55 | -3.77 | -3.64 | -1.74 | -3.29 | -2.50 | -7.54 | -6.92 | -3.82 |
Bud Neck | -1.82 | -10.51 | -12.58 | -12.03 | -0.55 | -3.51 | -14.02 | -6.74 | -6.40 | -2.86 | -5.62 | -17.51 | -14.72 | -13.81 | -4.85 |
Bud Site | 5.43 | 11.30 | -1.53 | -8.12 | -15.39 | -6.34 | -7.61 | -5.22 | -4.75 | -4.34 | -2.59 | 0.58 | -13.74 | -12.88 | -13.98 |
Cell Periphery | 2.44 | 1.95 | 4.51 | 5.12 | 7.85 | 2.45 | -5.98 | -4.82 | -4.89 | -4.48 | 1.34 | -4.53 | -3.08 | -3.76 | -0.87 |
Cytoplasm | -14.34 | 4.53 | -1.80 | 14.34 | -7.38 | 2.23 | 5.93 | 9.25 | 8.60 | 7.43 | -0.48 | 12.66 | 24.64 | 25.29 | 13.55 |
Cytoplasmic Foci | 12.91 | 21.73 | 8.71 | -4.79 | -17.70 | 2.02 | 25.55 | 5.04 | 4.36 | -1.95 | 7.83 | 32.56 | 2.17 | -2.94 | -17.18 |
Eisosomes | 1.37 | 3.81 | -0.36 | -2.18 | -5.36 | 1.18 | -2.15 | -2.54 | -2.58 | -2.25 | -0.20 | -0.77 | -6.09 | -5.99 | -4.93 |
Endoplasmic Reticulum | 5.16 | 8.69 | 9.18 | 9.45 | 2.52 | 6.38 | 13.81 | 13.98 | 8.57 | 2.28 | 7.30 | 16.17 | 18.04 | 13.99 | 4.86 |
Endosome | 7.02 | 12.42 | -5.81 | -10.91 | -14.14 | -6.42 | -5.33 | -2.73 | -1.74 | -1.86 | -2.55 | 3.27 | -13.00 | -12.49 | -13.68 |
Golgi | -1.00 | 0.95 | 6.50 | 9.17 | 7.27 | -8.73 | -0.74 | 0.20 | 2.99 | 0.41 | -10.56 | -3.29 | -1.53 | 6.03 | 0.71 |
Lipid Particles | -5.47 | -6.84 | -8.86 | -3.80 | -2.69 | 1.83 | -12.15 | -6.87 | -6.98 | -5.46 | -3.95 | -14.40 | -12.50 | -10.96 | -4.65 |
Mitochondria | 1.43 | 2.64 | 0.08 | -1.36 | -2.60 | 0.56 | 10.91 | 12.18 | 6.22 | 4.09 | 1.53 | 8.71 | -0.49 | -1.01 | -2.60 |
Mitotic Spindle | -1.82 | -5.97 | -9.56 | -8.72 | -7.38 | -5.91 | -9.41 | -3.12 | 0.25 | 5.62 | -5.70 | -11.83 | -10.12 | -9.27 | -7.46 |
None | -2.66 | -3.85 | 2.65 | 5.09 | 10.03 | 2.74 | 3.90 | 5.43 | 2.74 | 3.17 | 1.45 | 2.19 | 10.34 | 6.84 | 11.00 |
Nuclear Periphery | -0.62 | -13.21 | -3.84 | -3.85 | 12.26 | -0.80 | -12.05 | -3.28 | -2.76 | 4.13 | -1.60 | -15.12 | -5.40 | -4.71 | 11.24 |
Nuclear Periphery Foci | -6.87 | -17.59 | -5.00 | 2.55 | 14.52 | -1.46 | -14.10 | -1.35 | -0.95 | 5.63 | -5.94 | -21.67 | -4.66 | -0.83 | 13.96 |
Nucleolus | 0.22 | 0.89 | 0.83 | 0.86 | -1.71 | 0.30 | -7.69 | -3.68 | -3.71 | -0.46 | -0.34 | 0.24 | -0.51 | 0.03 | -3.80 |
Nucleus | -0.58 | -4.74 | -1.98 | -1.44 | 1.06 | 0.57 | -6.37 | -1.50 | -1.71 | 0.64 | 0.06 | -7.30 | -2.23 | -2.37 | 1.38 |
Peroxisomes | 1.73 | 2.96 | -4.03 | -7.91 | -9.53 | 2.83 | 2.87 | -1.96 | -2.18 | -2.19 | 2.14 | 4.41 | -8.13 | -8.82 | -9.35 |
Vacuole | 2.06 | -33.81 | -18.28 | -20.37 | 14.07 | -2.66 | -24.20 | -6.48 | -6.25 | -1.46 | -2.38 | -37.86 | -23.14 | -22.53 | 1.18 |
Vacuole Periphery | 10.82 | -1.50 | 12.81 | 4.17 | 19.59* | 2.41 | 8.37 | 10.15 | 8.28 | 4.86 | 7.80 | -0.35 | 13.56 | 7.13 | 13.42 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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