Standard name
Human Ortholog
Description RanGTP-binding protein; inhibits RanGAP1 (Rna1p)-mediated GTP hydrolysis of RanGTP (Gsp1p); shares similarity to proteins in other fungi but not in higher eukaryotes
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0 0.11 0 0 0 0 0 0
Cytoplasm 1.0 0.98 0.98 0.96 0.85 0.83 0.65 0.63 0.61 0.61 0.54 0.58 0.98 0.99 0.99 0.92 0.95 0.93 0.94 0.94 0.91 0.9 0.93 0.94
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0.05 0 0.22 0.24 0.32 0.52 0.44 0.55 0.53 0.6 0.56 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0.07 0.06 0.07 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 1 1 1 4 0 2 2 0 0 2 0 1 0 0 0 1 0 2 1
Bud 2 0 2 1 5 4 11 7 11 22 19 15 1 0 0 1 1 0 0 0 0 0 0 2
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 1 1
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 1 0 14 6 10 3 18 14 16 21 8 21 6 12 8 19 13 27 0 0 1 0 0 0
Cytoplasm 235 56 312 284 328 316 419 409 313 454 274 364 216 502 420 234 278 230 225 66 309 84 356 394
Endoplasmic Reticulum 0 0 3 0 3 2 19 4 6 17 7 19 4 4 0 14 8 7 8 0 10 0 2 4
Endosome 0 0 1 0 3 0 1 3 2 2 0 0 0 2 0 1 3 0 0 0 1 0 2 3
Golgi 0 0 1 0 1 0 0 0 0 0 0 0 0 0 0 3 1 4 0 0 1 2 2 0
Mitochondria 9 3 6 65 91 121 334 286 285 394 304 353 1 2 1 5 3 2 1 1 6 1 3 2
Nucleus 0 0 0 0 4 2 5 8 3 5 1 0 3 2 0 2 3 0 0 0 0 1 0 1
Nuclear Periphery 0 0 0 0 0 1 1 2 0 0 1 6 0 0 0 0 0 0 0 0 0 0 1 0
Nucleolus 0 0 0 1 0 1 1 0 1 0 2 0 0 0 0 0 0 1 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 1 3
Vac/Vac Membrane 0 0 1 3 5 6 14 43 30 50 22 28 0 3 2 12 12 11 1 0 2 0 3 2
Unique Cell Count 236 57 320 295 385 381 648 651 514 740 503 632 221 509 423 255 293 246 239 71 338 94 382 421
Labelled Cell Count 247 59 340 360 450 457 824 777 671 965 640 808 231 528 433 291 323 282 239 71 338 94 382 421


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 6.4 7.1 6.7 6.3 6.1 5.6 5.3 5.5 5.3 5.2 4.9 5.1 7.8 7.9 7.4 7.9 8.4 8.6 6.7 8.0 8.4
Std Deviation (1e-4) 0.7 1.0 0.8 1.1 1.3 0.9 1.0 1.3 1.1 1.2 1.1 1.0 1.1 0.9 1.0 1.2 1.1 1.8 0.9 1.2 1.3
Intensity Change (Log2) – – – -0.09 -0.13 -0.27 -0.34 -0.29 -0.34 -0.37 -0.45 -0.4 0.21 0.24 0.14 0.24 0.32 0.35 0.0 0.25 0.33


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 2.4 2.8 1.9 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery -1.6 -1.3 -3.1 -1.3 -1.9 -1.0 -1.3 -2.4 -0.8 -1.0 -1.6 -2.0 1.6 0 3.0
Cytoplasm -0.9 -5.6 -6.3 -11.2 -11.6 -11.9 -12.1 -13.3 -12.8 0.2 1.2 2.0 -3.1 -1.7 -2.3
Endoplasmic Reticulum 0 0 0 2.0 0 0 1.5 0 2.0 0 0 0 3.2 1.7 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 7.8 8.4 10.2 15.2 13.4 15.8 15.8 16.9 16.2 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 4.4 4.1 4.5 3.4 3.5 0 0 0 3.5 3.2 3.4

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 3.0608 4.1245 4.074 2.8459 3.1056 3.833 1.2958 2.7558 2.5705 2.0425 1.6074 2.4478 0.9083 2.3376 1.8155 1.9166 1.6484 1.7845
Actin 0.0386 0.0003 0.0006 0.0011 0.0001 0.0009 0.0136 0.0001 0.0052 0.0152 0.0146 0.0017 0.0012 0.0001 0.0002 0 0.0014 0.0002
Bud 0.0006 0.0003 0.0003 0.0004 0.0001 0.0001 0.0009 0.0002 0.0031 0.0095 0.0008 0.0004 0.0001 0.0002 0.0002 0.0001 0.0035 0.0003
Bud Neck 0.003 0.0001 0.0002 0.0004 0.0002 0.0007 0.0007 0.0001 0.0006 0.0007 0.0003 0.0005 0.0005 0.0001 0.0002 0.0001 0.0007 0.0005
Bud Periphery 0.0006 0.0001 0.0002 0.0002 0 0.0001 0.0008 0.0001 0.0025 0.0053 0.0013 0.0003 0.0001 0 0.0001 0 0.0022 0.0002
Bud Site 0.0021 0.0011 0.0007 0.0007 0.0001 0.0001 0.0043 0.0006 0.007 0.0015 0.0006 0.0001 0.0002 0.0003 0.0009 0.0001 0.0033 0
Cell Periphery 0.0004 0.0003 0.0003 0.0002 0 0.0002 0.0002 0.0001 0.0002 0.0003 0.0001 0.0001 0.0001 0.0001 0.0001 0 0.0002 0
Cytoplasm 0.7864 0.9654 0.8575 0.8939 0.9089 0.9007 0.7168 0.9262 0.8379 0.7874 0.693 0.9357 0.8864 0.9508 0.932 0.8705 0.8068 0.9387
Cytoplasmic Foci 0.0145 0.0058 0.0115 0.0196 0.0128 0.0143 0.0473 0.0146 0.0388 0.0258 0.0238 0.0142 0.0164 0.0081 0.0111 0.0195 0.0429 0.0144
Eisosomes 0.0001 0 0.0001 0 0 0.0001 0.0001 0 0 0.0001 0.0001 0 0 0 0 0 0 0
Endoplasmic Reticulum 0.01 0.0077 0.0088 0.0137 0.0019 0.0079 0.0145 0.0013 0.0046 0.0041 0.018 0.0039 0.0162 0.0053 0.0049 0.003 0.012 0.0047
Endosome 0.0289 0.0063 0.0242 0.0232 0.0155 0.0168 0.0609 0.0118 0.0428 0.068 0.0912 0.0193 0.0328 0.0133 0.0188 0.0768 0.0685 0.0233
Golgi 0.0106 0.0005 0.0014 0.0022 0.0003 0.002 0.0232 0.0005 0.008 0.0183 0.0231 0.0013 0.0016 0.0005 0.0005 0.0008 0.0018 0.0005
Lipid Particles 0.0014 0.0001 0.0003 0.0002 0.0001 0.0002 0.0262 0.0003 0.0022 0.0059 0.0441 0.0001 0.0006 0.0001 0.0001 0.0001 0.0008 0.0001
Mitochondria 0.0024 0.0002 0.0004 0.0004 0.0002 0.0019 0.0215 0.0001 0.0022 0.0148 0.0195 0.0007 0.0008 0.0001 0.0002 0.0002 0.0022 0.0004
None 0.0446 0.0063 0.0853 0.0355 0.0535 0.0499 0.027 0.0396 0.0211 0.019 0.0066 0.01 0.0294 0.0153 0.023 0.0042 0.0141 0.0106
Nuclear Periphery 0.0324 0.0007 0.0012 0.0011 0.0004 0.0003 0.0032 0.0003 0.0064 0.0052 0.0301 0.0024 0.0038 0.0007 0.001 0.0013 0.0166 0.0006
Nucleolus 0.0003 0 0 0 0 0 0.0004 0 0.0001 0.0003 0.0002 0.0002 0.0001 0 0 0 0.0006 0
Nucleus 0.0135 0.0007 0.0008 0.0011 0.0006 0.0004 0.0017 0.0006 0.0033 0.0028 0.0024 0.0048 0.0013 0.0007 0.001 0.0012 0.0064 0.001
Peroxisomes 0.0012 0 0.0001 0.0001 0 0.0004 0.0228 0 0.0044 0.0034 0.0064 0.0005 0.0001 0 0 0 0.0006 0
Punctate Nuclear 0.0044 0.0001 0.0003 0.0006 0.0002 0.0001 0.0043 0.0002 0.0007 0.003 0.0017 0.0007 0.0004 0.0001 0.0002 0.0001 0.0044 0.0004
Vacuole 0.0029 0.0039 0.0048 0.0046 0.0041 0.0019 0.0075 0.0029 0.0072 0.0067 0.0106 0.0025 0.0068 0.0037 0.0046 0.0176 0.0077 0.0031
Vacuole Periphery 0.0011 0.0003 0.0008 0.0007 0.001 0.0007 0.0021 0.0004 0.0015 0.0028 0.0116 0.0007 0.0011 0.0005 0.0008 0.0043 0.0035 0.0008

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 5.4211 3.7278 8.8518 9.4411 8.6507 7.502 8.1037 6.6142 14.4743 8.1587
Translational Efficiency 2.6605 3.1095 1.2181 2.0226 1.6314 1.9586 1.4773 1.8805 1.0661 1.9523

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
65 160 1591 257 718 2051 432 1378 783 2211 2023 1635

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 937.90 985.98 1008.57 1051.02 815.57 857.94 1083.29 993.98 825.73 867.21 1024.53 1002.95
Standard Deviation 142.06 149.62 134.14 142.23 117.77 107.76 130.26 133.58 124.63 116.16 136.79 136.56
Intensity Change Log 2 — 0.072124 0.104805 0.164284 — 0.073068 0.409539 0.285408 — 0.072563 0.254574 0.221888

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.001888 0.000964 0.000101 0.000308 0.000343 0.000328 0.000209 0.001209 0.000471 0.000374 0.000124 0.001067
Bud Neck 0.004032 0.007718 0.000708 0.001587 0.004388 0.008212 0.001274 0.002382 0.004358 0.008176 0.000829 0.002257
Bud Site 0.092693 0.056618 0.003649 0.024621 0.014639 0.028561 0.009850 0.039993 0.021119 0.030591 0.004973 0.037577
Cell Periphery 0.000741 0.000222 0.000058 0.000104 0.000202 0.000150 0.000126 0.000096 0.000247 0.000155 0.000073 0.000098
Cytoplasm 0.385372* 0.533101* 0.838948* 0.669174* 0.573235* 0.593463* 0.625305* 0.708137* 0.557640* 0.589095* 0.793326* 0.702012*
Cytoplasmic Foci 0.235169* 0.260381* 0.042745 0.095402 0.258557* 0.179874 0.071190 0.062123 0.256615* 0.185700* 0.048820 0.067354
Eisosomes 0.000117 0.000065 0.000011 0.000021 0.000080 0.000050 0.000043 0.000019 0.000083 0.000051 0.000018 0.000019
Endoplasmic Reticulum 0.011676 0.001283 0.000668 0.000342 0.005464 0.000793 0.000866 0.000358 0.005980 0.000828 0.000710 0.000356
Endosome 0.026727 0.029081 0.003094 0.003945 0.029175 0.027341 0.009127 0.007002 0.028972 0.027467 0.004382 0.006522
Golgi 0.021293 0.006583 0.000201 0.000402 0.010084 0.011708 0.000209 0.007948 0.011014 0.011337 0.000202 0.006762
Lipid Particles 0.020883 0.001721 0.000492 0.000904 0.004616 0.001325 0.001416 0.000494 0.005966 0.001353 0.000689 0.000558
Mitochondria 0.029391 0.000693 0.000033 0.000345 0.001406 0.003001 0.000060 0.002192 0.003729 0.002834 0.000039 0.001902
Mitotic Spindle 0.020533 0.002042 0.000588 0.002144 0.001857 0.005330 0.002166 0.022538 0.003407 0.005092 0.000925 0.019333
None 0.001883 0.000120 0.003693 0.000916 0.001478 0.000435 0.001955 0.001910 0.001512 0.000413 0.003322 0.001754
Nuclear Periphery 0.001657 0.000230 0.000127 0.000137 0.000268 0.000064 0.000292 0.000173 0.000384 0.000076 0.000162 0.000167
Nuclear Periphery Foci 0.014076 0.001777 0.002966 0.002211 0.000918 0.000364 0.007107 0.001390 0.002010 0.000466 0.003851 0.001519
Nucleolus 0.006218 0.000140 0.000125 0.000111 0.000198 0.000196 0.000163 0.000124 0.000698 0.000192 0.000133 0.000122
Nucleus 0.065462 0.005948 0.037556 0.015852 0.010830 0.008609 0.031130 0.012532 0.015366 0.008417 0.036184 0.013054
Peroxisomes 0.005579 0.004816 0.000170 0.001215 0.001355 0.002854 0.000320 0.001801 0.001705 0.002996 0.000202 0.001709
Vacuole 0.033439 0.083345 0.063855 0.179966 0.073169 0.120908 0.236959* 0.124702 0.069871 0.118190 0.100820 0.133389
Vacuole Periphery 0.021170 0.003153 0.000213 0.000292 0.007738 0.006435 0.000231 0.002878 0.008853 0.006198 0.000217 0.002472

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin 2.10 4.98 4.47 2.28 -3.72 0.13 1.50 -1.11 -1.17 -1.68 0.85 3.90 -0.51 -0.94 -2.09
Bud Neck -2.24 3.80 3.12 4.74 -2.95 -5.18 5.77 4.84 11.83 -2.20 -5.51 6.94 5.33 12.65 -4.16
Bud Site 1.86 4.91 4.16 4.77 -3.87 -8.06 3.83 -3.46 2.08 -5.83 -3.96 8.13 -0.36 3.57 -8.70
Cell Periphery 2.73 3.42 3.33 4.10 -2.39 1.54 5.61 7.42 2.29 3.03 2.74 7.60 7.05 2.61 -0.43
Cytoplasm -3.48 -11.62 -4.54 -1.50 13.37 -1.80 -0.95 -7.12 -6.86 -4.65 -2.85 -20.23 -7.54 -6.28 14.88
Cytoplasmic Foci -0.81 7.37 6.06 11.70 -4.91 10.06 25.07 28.81 28.91 3.50 9.36 30.54 28.99 30.38 -2.66
Eisosomes 1.92 4.00 3.56 4.34 -5.42 3.37 4.24 7.28 9.79 9.19 3.79 8.02 7.94 10.45 -0.48
Endoplasmic Reticulum 3.68 3.96 4.06 1.83 2.37 4.48 4.48 5.05 4.11 6.49 5.22 5.40 5.84 4.42 4.62
Endosome -0.29 4.65 4.68 5.07 0.36 0.88 10.93 12.87 18.92 4.40 0.77 13.87 13.70 19.88 0.35
Golgi 3.54 5.19 5.19 6.04 -0.62 -1.17 8.55 4.35 6.79 -3.43 -0.22 9.67 5.78 7.88 -3.42
Lipid Particles 1.81 1.93 1.88 1.56 -3.13 6.33 6.30 8.04 8.24 4.94 4.56 5.27 5.36 7.79 1.35
Mitochondria 2.21 2.26 2.25 1.20 -0.96 -2.10 2.99 0.62 2.60 -2.02 0.69 3.13 2.30 2.85 -2.10
Mitotic Spindle 2.23 2.43 2.18 -0.25 -1.42 -2.31 0.32 -4.55 -3.00 -4.82 -0.99 2.50 -3.45 -2.81 -5.65
None 2.16 -1.66 1.27 -4.20 5.81 2.37 -0.12 -0.07 -2.61 0.03 2.69 -2.88 0.19 -2.84 3.34
Nuclear Periphery 2.09 2.30 2.11 -0.15 -2.40 1.17 -0.38 0.32 -4.69 3.80 1.81 1.22 0.98 -4.52 -1.76
Nuclear Periphery Foci 1.85 1.70 1.82 -0.37 2.37 2.13 -10.21 -1.44 -3.01 9.70 2.55 -3.20 1.14 -3.44 10.51
Nucleolus 2.07 2.07 2.08 0.94 2.45 0.09 2.02 3.31 4.44 1.39 2.02 2.27 2.34 4.91 1.16
Nucleus 2.48 1.15 1.91 -6.31 7.90 1.75 -11.36 -6.27 -11.99 8.06 2.94 -8.79 -1.64 -13.13 14.46
Peroxisomes 0.32 2.08 1.86 4.72 -4.68 -4.66 8.97 1.57 5.20 -3.66 -3.43 6.15 2.55 6.18 -4.58
Vacuole -4.70 -5.72 -13.87 -10.45 -12.28 -9.36 -16.15 -19.54 -12.81 4.42 -10.11 -8.94 -22.83 -15.61 -15.33
Vacuole Periphery 2.97 3.55 3.55 2.06 1.22 0.91 5.46 4.41 8.06 -2.36 1.87 6.34 5.44 8.88 -2.32
Standard name
Human Ortholog
Description RanGTP-binding protein; inhibits RanGAP1 (Rna1p)-mediated GTP hydrolysis of RanGTP (Gsp1p); shares similarity to proteins in other fungi but not in higher eukaryotes
Localization
Cell Percentages cytoplasm (97%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-4

Yrb30

Yrb30


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Yrb30-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available