Standard name | |
---|---|
Human Ortholog | |
Description | Central component of the peroxisomal importomer complex; peroxisomal protein import machinery docking complex component; interacts with both PTS1 (Pex5p) and PTS2 (Pex7p) peroxisomal matrix protein signal recognition factors and membrane receptor Pex13p |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 4 | 9 | 7 | 2 | 2 | 31 | 15 | 31 | – | 8 | 13 | 9 | 15 | 34 | 21 | 4 | 0 | 2 | 7 | 11 | 3 | 1 | 11 | 29 |
Bud | 11 | 14 | 13 | 21 | 56 | 59 | 77 | 97 | – | 60 | 47 | 64 | 9 | 26 | 24 | 7 | 4 | 6 | 0 | 2 | 0 | 1 | 5 | 2 |
Bud Neck | 0 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | – | 1 | 0 | 2 | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 2 | 0 | 1 | 3 | 2 |
Bud Site | 10 | 5 | 5 | 9 | 18 | 24 | 26 | 14 | – | 29 | 24 | 19 | 1 | 6 | 4 | 3 | 4 | 4 | – | – | – | – | – | – |
Cell Periphery | 0 | 2 | 0 | 0 | 0 | 1 | 3 | 0 | – | 1 | 3 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 0 | 0 | 0 | 8 | 12 | 6 | 11 | 19 | – | 13 | 9 | 12 | 1 | 4 | 4 | 6 | 3 | 4 | 0 | 0 | 1 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0 | 0 | 1 | 2 | 2 | 0 | 0 | 1 | – | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 3 | 0 |
Endosome | 10 | 4 | 1 | 4 | 14 | 0 | 11 | 16 | – | 19 | 7 | 14 | 4 | 20 | 23 | 6 | 5 | 6 | 3 | 8 | 6 | 5 | 13 | 9 |
Golgi | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 3 | 3 | 3 | 3 | 2 | 3 | 9 | 1 | 1 | 6 | 8 |
Mitochondria | 106 | 140 | 78 | 84 | 156 | 259 | 287 | 297 | – | 201 | 238 | 261 | 54 | 104 | 91 | 11 | 7 | 18 | 4 | 11 | 2 | 3 | 11 | 11 |
Nucleus | 1 | 0 | 0 | 0 | 0 | 1 | 2 | 3 | – | 0 | 3 | 0 | 0 | 1 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 2 | 3 | 1 | 0 | 6 | 6 | 15 | 1 | – | 10 | 5 | 8 | 0 | 2 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 18 | 12 | 4 | 11 | 15 | 10 | 33 | 28 | – | 32 | 15 | 10 | 7 | 6 | 9 | 25 | 23 | 17 | 0 | 1 | 0 | 2 | 0 | 0 |
Peroxisomes | 175 | 148 | 103 | 171 | 244 | 220 | 362 | 388 | – | 204 | 195 | 255 | 72 | 326 | 311 | 124 | 97 | 93 | 205 | 180 | 119 | 79 | 246 | 232 |
SpindlePole | 24 | 17 | 24 | 40 | 62 | 33 | 103 | 114 | – | 100 | 50 | 70 | 14 | 61 | 61 | 37 | 27 | 29 | 13 | 10 | 10 | 1 | 9 | 6 |
Vac/Vac Membrane | 1 | 2 | 1 | 0 | 0 | 0 | 0 | 0 | – | 4 | 2 | 3 | 0 | 2 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 1 |
Unique Cell Count | 237 | 225 | 145 | 223 | 351 | 376 | 571 | 620 | 415 | 387 | 458 | 106 | 380 | 368 | 173 | 133 | 132 | 241 | 240 | 151 | 103 | 318 | 306 | |
Labelled Cell Count | 363 | 357 | 239 | 353 | 588 | 650 | 946 | 1010 | 682 | 611 | 729 | 177 | 597 | 553 | 228 | 174 | 184 | 241 | 240 | 151 | 103 | 318 | 306 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 9.5 | 10.1 | 6.6 | 6.7 | 5.9 | 5.7 | 5.1 | 5.4 | – | 5.0 | 5.3 | 5.4 | 8.4 | 8.8 | 8.8 | 12.6 | 14.4 | 14.2 | 9.1 | 10.1 | 10.1 |
Std Deviation (1e-4) | 2.9 | 2.7 | 1.3 | 2.3 | 1.7 | 1.5 | 1.5 | 1.5 | – | 1.6 | 1.6 | 1.5 | 1.8 | 1.8 | 1.7 | 5.1 | 5.3 | 5.4 | 2.3 | 2.5 | 2.3 |
Intensity Change (Log2) | – | – | – | 0.03 | -0.15 | -0.22 | -0.37 | -0.28 | – | -0.4 | -0.32 | -0.29 | 0.35 | 0.41 | 0.43 | 0.94 | 1.13 | 1.11 | 0.46 | 0.61 | 0.62 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 1.3 | 0 | 0.1 | – | 0 | -0.8 | 0 | 2.6 | 1.6 | 0.4 | -1.2 | 0 | 0 |
Bud | 0.1 | 2.0 | 2.0 | 1.5 | 2.1 | – | 1.7 | 1.0 | 1.6 | -0.1 | -0.8 | -1.0 | -1.8 | -2.1 | -1.5 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0.3 | 0.8 | 1.3 | 0.6 | 0 | – | 1.5 | 1.2 | 0.4 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | – | 2.2 | 0 | 0 | 0 | 2.4 | 2.7 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | -3.0 | -1.9 | 3.2 | -0.8 | -1.3 | – | -1.1 | 1.6 | 0.7 | -0.4 | -5.7 | -6.3 | -9.4 | -8.8 | -7.0 |
Nucleus | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 1.0 | 0.8 | 0 | 1.5 | 1.0 | – | 2.1 | 0.6 | 0 | 0 | 0 | 0 | 3.6 | 4.1 | 3.2 |
Peroxisomes | 1.2 | -0.3 | -2.6 | -1.7 | -1.9 | – | -4.6 | -4.3 | -3.3 | -0.5 | 3.9 | 3.5 | 0.1 | 0.4 | -0.1 |
SpindlePole | 0.3 | 0.3 | -2.5 | 0.4 | 0.5 | – | 1.9 | -1.1 | -0.4 | -0.7 | -0.1 | 0 | 1.1 | 0.8 | 1.1 |
Vacuole | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 4.5798 | 3.2973 | 3.8115 | 3.5409 | 3.2405 | 3.6429 | 5.4978 | 4.2791 | 5.0864 | 4.4323 | 4.8092 | 4.7411 | 3.5378 | 2.9035 | 2.5444 | 2.9766 | 2.5064 | 2.9687 |
Actin | 0.005 | 0.0128 | 0.0209 | 0.0014 | 0.0007 | 0.0018 | 0.016 | 0.0196 | 0.0126 | 0.0015 | 0.0132 | 0.0021 | 0.0195 | 0.0166 | 0.0158 | 0.0013 | 0.0006 | 0.0044 |
Bud | 0.0003 | 0.0004 | 0.0032 | 0.0003 | 0.0043 | 0 | 0.0001 | 0.0005 | 0.0027 | 0.0006 | 0 | 0 | 0.0002 | 0.0011 | 0.0032 | 0.006 | 0 | 0.0001 |
Bud Neck | 0.0004 | 0.0003 | 0.0011 | 0.0002 | 0.0002 | 0.0002 | 0.0018 | 0.0004 | 0.0034 | 0.0001 | 0.0002 | 0.0001 | 0.0032 | 0.0008 | 0.0022 | 0.0001 | 0.0001 | 0.0013 |
Bud Periphery | 0.0005 | 0.0003 | 0.0039 | 0.0007 | 0.0045 | 0 | 0.0001 | 0.0004 | 0.0029 | 0.0007 | 0 | 0 | 0.0002 | 0.0013 | 0.0042 | 0.0069 | 0 | 0.0004 |
Bud Site | 0.0015 | 0.0057 | 0.0062 | 0.0005 | 0.0098 | 0.0002 | 0.0038 | 0.0226 | 0.0045 | 0.0026 | 0.0004 | 0.0001 | 0.0032 | 0.0229 | 0.0023 | 0.0032 | 0.0001 | 0.0003 |
Cell Periphery | 0.0001 | 0.0001 | 0.0001 | 0 | 0 | 0 | 0 | 0.0001 | 0.0001 | 0 | 0 | 0 | 0.0001 | 0.0001 | 0.0001 | 0 | 0 | 0 |
Cytoplasm | 0.0044 | 0.0002 | 0.0002 | 0 | 0.0002 | 0 | 0.001 | 0.0001 | 0.0002 | 0 | 0.0017 | 0 | 0.0003 | 0.0001 | 0.0002 | 0.0002 | 0.0002 | 0.0001 |
Cytoplasmic Foci | 0.1028 | 0.1037 | 0.1101 | 0.0901 | 0.1201 | 0.1038 | 0.0822 | 0.1398 | 0.0884 | 0.0753 | 0.1447 | 0.0786 | 0.0972 | 0.1284 | 0.093 | 0.0993 | 0.1595 | 0.1308 |
Eisosomes | 0.0001 | 0 | 0.0001 | 0 | 0 | 0 | 0 | 0 | 0.0001 | 0 | 0 | 0 | 0.0001 | 0 | 0.0001 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0.0025 | 0 | 0.0001 | 0 | 0.0001 | 0 | 0.0005 | 0 | 0.0005 | 0 | 0.0012 | 0 | 0.0001 | 0 | 0.0001 | 0 | 0 | 0.0001 |
Endosome | 0.0135 | 0.0096 | 0.0066 | 0.0055 | 0.0259 | 0.0046 | 0.0056 | 0.0027 | 0.0046 | 0.0015 | 0.0128 | 0.0025 | 0.0038 | 0.0058 | 0.0046 | 0.0018 | 0.0353 | 0.0032 |
Golgi | 0.0102 | 0.016 | 0.0113 | 0.0195 | 0.0108 | 0.0121 | 0.0119 | 0.0088 | 0.009 | 0.0069 | 0.0224 | 0.0089 | 0.0136 | 0.0107 | 0.0087 | 0.0079 | 0.0062 | 0.0075 |
Lipid Particles | 0.1263 | 0.0594 | 0.0819 | 0.0515 | 0.1611 | 0.152 | 0.1415 | 0.0467 | 0.0801 | 0.1278 | 0.1585 | 0.131 | 0.161 | 0.1133 | 0.0608 | 0.2068 | 0.1304 | 0.1553 |
Mitochondria | 0.0199 | 0.0226 | 0.0184 | 0.02 | 0.0204 | 0.0172 | 0.02 | 0.0087 | 0.0206 | 0.014 | 0.0126 | 0.0106 | 0.0317 | 0.0175 | 0.0195 | 0.0247 | 0.0052 | 0.0127 |
None | 0.0008 | 0.0001 | 0.0006 | 0.0001 | 0.0001 | 0 | 0.0004 | 0.0001 | 0.0002 | 0 | 0.0025 | 0 | 0.0003 | 0.0001 | 0.0003 | 0.0001 | 0 | 0.0002 |
Nuclear Periphery | 0.0042 | 0 | 0.0001 | 0.0001 | 0.0001 | 0 | 0.0003 | 0 | 0.0003 | 0 | 0.0008 | 0 | 0.0001 | 0 | 0.0002 | 0 | 0 | 0.0008 |
Nucleolus | 0.0073 | 0.0012 | 0.0041 | 0.0003 | 0.0026 | 0.0004 | 0.0048 | 0.0062 | 0.0006 | 0.0006 | 0.0002 | 0.0002 | 0.0034 | 0.0029 | 0.0006 | 0.0003 | 0.0003 | 0.0023 |
Nucleus | 0.0059 | 0.0001 | 0.0004 | 0 | 0.0003 | 0 | 0.0002 | 0.0005 | 0.0002 | 0.0001 | 0.0001 | 0 | 0.0002 | 0.0006 | 0.0001 | 0.0001 | 0 | 0.0002 |
Peroxisomes | 0.6775 | 0.755 | 0.716 | 0.8045 | 0.636 | 0.7058 | 0.7057 | 0.7219 | 0.7662 | 0.7645 | 0.6264 | 0.7644 | 0.6584 | 0.6741 | 0.7815 | 0.6402 | 0.65 | 0.677 |
Punctate Nuclear | 0.0154 | 0.0109 | 0.0094 | 0.0051 | 0.0018 | 0.0015 | 0.0036 | 0.0202 | 0.0021 | 0.0035 | 0.0017 | 0.0015 | 0.0032 | 0.0017 | 0.0021 | 0.0008 | 0.0024 | 0.0027 |
Vacuole | 0.0011 | 0.0011 | 0.0041 | 0.0001 | 0.0005 | 0.0001 | 0.0003 | 0.0004 | 0.0006 | 0.0001 | 0.0002 | 0.0001 | 0.0002 | 0.0014 | 0.0003 | 0.0003 | 0.0071 | 0.0003 |
Vacuole Periphery | 0.0006 | 0.0004 | 0.0013 | 0.0001 | 0.0004 | 0.0001 | 0.0002 | 0.0002 | 0.0003 | 0.0001 | 0.0002 | 0.0001 | 0.0002 | 0.0005 | 0.0001 | 0.0001 | 0.0023 | 0.0002 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 13.443 | 20.189 | 9.9874 | 14.1547 | 18.1267 | 13.973 | 20.8991 | 16.7298 | 20.8902 | 17.6674 |
Translational Efficiency | 1.8627 | 1.1352 | 2.5052 | 1.9081 | 1.4991 | 1.9328 | 0.9313 | 1.5156 | 1.3531 | 1.5104 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
1972 | 1378 | 305 | 860 | 1957 | 1823 | 171 | 1194 | 3929 | 3201 | 476 | 2054 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 838.46 | 904.21 | 1287.29 | 1235.13 | 839.71 | 971.99 | 1168.15 | 1136.14 | 839.08 | 942.81 | 1244.49 | 1177.59 |
Standard Deviation | 134.00 | 180.24 | 203.16 | 194.92 | 142.00 | 185.41 | 162.69 | 223.21 | 138.05 | 186.25 | 198.05 | 217.38 |
Intensity Change Log 2 | — | 0.108916 | 0.618523 | 0.558849 | — | 0.211050 | 0.476262 | 0.436178 | — | 0.160925 | 0.549093 | 0.498771 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.026563 | 0.032146 | 0.048837 | 0.391356* | 0.019636 | 0.026082 | 0.056437 | 0.319252* | 0.023113 | 0.028692 | 0.051567 | 0.349442* |
Bud Neck | 0.001287 | 0.001880 | 0.008743 | 0.004229 | 0.001485 | 0.002817 | 0.005309 | 0.002465 | 0.001386 | 0.002414 | 0.007510 | 0.003203 |
Bud Site | 0.002496 | 0.004007 | 0.013018 | 0.014176 | 0.003616 | 0.007389 | 0.018078 | 0.008725 | 0.003054 | 0.005933 | 0.014835 | 0.011007 |
Cell Periphery | 0.000127 | 0.000098 | 0.000098 | 0.000160 | 0.000121 | 0.000109 | 0.000090 | 0.000159 | 0.000124 | 0.000104 | 0.000095 | 0.000159 |
Cytoplasm | 0.002252 | 0.003189 | 0.003458 | 0.002895 | 0.003134 | 0.003908 | 0.002623 | 0.003415 | 0.002691 | 0.003599 | 0.003158 | 0.003198 |
Cytoplasmic Foci | 0.048014 | 0.047941 | 0.105378 | 0.039213 | 0.043324 | 0.059829 | 0.069529 | 0.030687 | 0.045678 | 0.054711 | 0.092500 | 0.034257 |
Eisosomes | 0.002638 | 0.009324 | 0.003344 | 0.003006 | 0.004649 | 0.002927 | 0.001571 | 0.005780 | 0.003639 | 0.005681 | 0.002707 | 0.004618 |
Endoplasmic Reticulum | 0.000218 | 0.000121 | 0.000380 | 0.000594 | 0.000080 | 0.000189 | 0.000351 | 0.000725 | 0.000149 | 0.000159 | 0.000369 | 0.000671 |
Endosome | 0.006590 | 0.004159 | 0.006725 | 0.014443 | 0.004491 | 0.007200 | 0.009452 | 0.009489 | 0.005545 | 0.005891 | 0.007705 | 0.011563 |
Golgi | 0.058116 | 0.046575 | 0.036106 | 0.060624 | 0.042753 | 0.063175 | 0.021434 | 0.045678 | 0.050464 | 0.056029 | 0.030835 | 0.051936 |
Lipid Particles | 0.048923 | 0.039285 | 0.040455 | 0.034879 | 0.029335 | 0.046436 | 0.018706 | 0.025154 | 0.039166 | 0.043357 | 0.032642 | 0.029226 |
Mitochondria | 0.013845 | 0.014334 | 0.028488 | 0.032446 | 0.018006 | 0.039073 | 0.005139 | 0.032595 | 0.015918 | 0.028423 | 0.020100 | 0.032532 |
Mitotic Spindle | 0.002904 | 0.003338 | 0.048143 | 0.012221 | 0.001806 | 0.007613 | 0.043117 | 0.003992 | 0.002357 | 0.005773 | 0.046337 | 0.007437 |
None | 0.000222 | 0.000245 | 0.000180 | 0.000165 | 0.000187 | 0.000223 | 0.000080 | 0.000179 | 0.000205 | 0.000232 | 0.000144 | 0.000173 |
Nuclear Periphery | 0.000036 | 0.000047 | 0.000365 | 0.000155 | 0.000030 | 0.000087 | 0.000107 | 0.000074 | 0.000033 | 0.000070 | 0.000272 | 0.000108 |
Nuclear Periphery Foci | 0.000606 | 0.000326 | 0.000410 | 0.000165 | 0.000203 | 0.000402 | 0.000167 | 0.000162 | 0.000405 | 0.000370 | 0.000323 | 0.000163 |
Nucleolus | 0.000160 | 0.000180 | 0.000449 | 0.000061 | 0.000098 | 0.000304 | 0.000119 | 0.000120 | 0.000129 | 0.000251 | 0.000330 | 0.000095 |
Nucleus | 0.000099 | 0.000048 | 0.004686 | 0.000041 | 0.000033 | 0.000140 | 0.000114 | 0.000047 | 0.000066 | 0.000101 | 0.003044 | 0.000045 |
Peroxisomes | 0.784409* | 0.792394* | 0.648140* | 0.385313* | 0.826798* | 0.731294* | 0.744531* | 0.509138* | 0.805523* | 0.757597* | 0.682768* | 0.457293* |
Vacuole | 0.000382 | 0.000323 | 0.002251 | 0.002449 | 0.000186 | 0.000622 | 0.002984 | 0.001250 | 0.000284 | 0.000493 | 0.002514 | 0.001752 |
Vacuole Periphery | 0.000113 | 0.000040 | 0.000346 | 0.001408 | 0.000030 | 0.000182 | 0.000061 | 0.000915 | 0.000072 | 0.000121 | 0.000244 | 0.001121 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -1.90 | -3.01 | -30.29* | -29.53* | -24.69* | -2.86 | -4.01 | -29.96* | -29.15* | -19.63* | -3.07 | -4.96 | -42.18* | -41.16* | -31.44* |
Bud Neck | -1.71 | -5.94 | -6.63 | -4.65 | 3.43 | -3.62 | -4.77 | -3.19 | 0.80 | 3.53 | -4.06 | -7.17 | -7.10 | -2.76 | 4.92 |
Bud Site | -1.58 | -5.41 | -5.10 | -4.28 | -0.39 | -3.42 | -3.19 | -4.23 | -1.09 | 2.08 | -3.87 | -5.64 | -6.73 | -4.01 | 1.74 |
Cell Periphery | 1.08 | 1.21 | -1.27 | -2.85 | -3.46 | 0.54 | 1.09 | -1.38 | -2.31 | -2.73 | 1.15 | 1.63 | -1.83 | -3.55 | -4.17 |
Cytoplasm | -2.32 | -1.89 | -1.83 | 0.57 | 0.85 | -2.07 | 0.71 | -0.81 | 1.27 | -1.34 | -3.33 | -1.28 | -2.04 | 1.30 | 0.16 |
Cytoplasmic Foci | -0.01 | -6.37 | 2.34 | 2.24 | 7.15 | -4.73 | -2.94 | 4.19 | 8.34 | 4.30 | -3.73 | -7.06 | 4.87 | 7.91 | 8.67 |
Eisosomes | -6.91 | -1.40 | -0.98 | 6.42 | 0.62 | 2.86 | 4.79 | -1.17 | -3.07 | -4.41 | -3.85 | 2.30 | -1.71 | 1.56 | -3.20 |
Endoplasmic Reticulum | 1.47 | -1.90 | -3.18 | -4.85 | -1.64 | -4.99 | -2.10 | -1.39 | -1.15 | -0.64 | -0.27 | -3.22 | -1.90 | -1.88 | -0.94 |
Endosome | 3.04 | -0.10 | -4.88 | -6.51 | -4.32 | -2.91 | -2.39 | -3.94 | -1.78 | -0.00 | -0.55 | -2.05 | -6.04 | -5.67 | -2.95 |
Golgi | 3.47 | 5.05 | -0.56 | -3.06 | -4.57 | -6.72 | 6.08 | -0.85 | 4.60 | -5.77 | -2.44 | 6.50 | -0.52 | 1.41 | -5.94 |
Lipid Particles | 2.45 | 1.40 | 3.44 | 1.08 | 0.84 | -5.01 | 2.41 | 1.32 | 5.82 | -1.37 | -1.59 | 1.58 | 3.86 | 5.16 | 0.77 |
Mitochondria | -0.26 | -2.79 | -6.07 | -5.66 | -0.70 | -7.10 | 7.24 | -4.72 | 1.71 | -9.27 | -6.70 | -1.19 | -7.59 | -1.59 | -3.24 |
Mitotic Spindle | -0.44 | -6.12 | -4.46 | -4.03 | 4.70 | -5.54 | -4.63 | -3.16 | 2.96 | 4.37 | -4.70 | -7.69 | -5.26 | -1.54 | 6.72 |
None | -0.61 | 1.25 | 1.73 | 4.93 | 0.83 | -1.66 | 6.77 | 0.53 | 2.00 | -6.03 | -1.32 | 3.09 | 1.72 | 4.04 | -2.21 |
Nuclear Periphery | -2.39 | -6.62 | -4.41 | -4.02 | 3.73 | -11.61 | -4.87 | -2.26 | 0.60 | 1.36 | -10.37 | -7.32 | -4.66 | -2.37 | 4.52 |
Nuclear Periphery Foci | 1.22 | 0.92 | 2.05 | 1.61 | 2.59 | -2.56 | 0.34 | 0.44 | 2.26 | 0.23 | 0.32 | 0.70 | 2.03 | 2.76 | 2.15 |
Nucleolus | -0.23 | -2.11 | 1.80 | 3.01 | 3.03 | -2.69 | -0.55 | -0.64 | 1.72 | -0.26 | -2.20 | -2.32 | 0.55 | 2.49 | 2.53 |
Nucleus | 1.33 | -1.42 | 1.53 | 0.64 | 1.43 | -1.99 | -1.42 | -1.49 | 1.63 | 1.18 | -0.95 | -1.43 | 0.97 | 1.70 | 1.44 |
Peroxisomes | -0.95 | 7.67 | 31.54* | 31.09* | 12.44 | 12.06 | 4.29 | 28.65* | 18.54 | 10.43 | 8.27 | 9.16 | 41.10* | 33.48 | 14.37 |
Vacuole | 0.40 | -2.58 | -2.59 | -2.69 | -0.13 | -4.97 | -1.19 | -2.69 | -1.58 | 0.87 | -2.44 | -2.07 | -3.62 | -3.12 | 0.91 |
Vacuole Periphery | 1.04 | -2.10 | -2.16 | -2.30 | -1.76 | -2.48 | -2.64 | -2.23 | -1.82 | -2.14 | -0.98 | -2.63 | -3.07 | -2.93 | -2.54 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
---|
Gene | Images |
---|