Standard name
Human Ortholog
Description Component of the RNA polymerase II mediator complex; mediator is required for transcriptional activation and also has a role in basal transcription
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 – 0 0.05 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0 0 0 0 – 0 0 0.09 0.13 0.14 0 0.07 0.06 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0.15 0.22 0.13 0.34 0.32 0.39 0.5 – 0.47 0.5 0 0 0 0 0 0.1 0 0 0 0 0 0
Nucleus 0.91 0.9 0.9 0.91 0.89 0.84 0.79 0.74 0.69 – 0.74 0.7 0.88 0.81 0.81 0.84 0.85 0.89 0.73 0.86 0.84 0.85 0.71 0.63
Nuclear Periphery 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.21 0.13 0 0.09 0.09 0.13 0.15 0.18 0.22 – 0.25 0.31 0 0.07 0.06 0 0 0.05 0.19 0.09 0.06 0.07 0.14 0.19
Peroxisomes 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0.05 0 0 0 0 0 0.07 0.09
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 1 0 0 0 0 0 0 0 0
Bud 0 0 1 0 0 1 4 8 4 – 6 4 0 0 0 2 1 1 0 2 2 0 1 2
Bud Neck 0 0 1 0 0 1 0 1 1 – 1 1 0 0 0 1 1 1 0 0 0 0 0 0
Bud Site 0 0 5 2 4 18 23 22 17 – 9 17 0 0 4 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 – 0 0 0 0 1 1 0 0 0 0 0 0 0 0
Cytoplasm 2 8 12 7 14 8 19 24 8 – 11 10 20 40 49 12 16 12 0 0 0 0 0 1
Endoplasmic Reticulum 0 0 1 1 0 0 0 0 0 – 0 0 0 1 1 3 6 6 0 0 3 0 2 1
Endosome 2 2 0 0 0 0 0 0 0 – 0 0 0 1 2 5 0 0 2 3 5 1 2 6
Golgi 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 4 2 0 0 0 3 0 2 1
Mitochondria 0 7 65 74 61 147 189 334 204 – 163 187 2 0 4 6 2 20 0 2 4 0 3 4
Nucleus 117 318 389 312 423 366 468 639 279 – 259 260 185 244 281 227 181 175 109 327 386 151 162 167
Nuclear Periphery 0 3 4 3 4 4 15 10 7 – 10 8 1 1 4 7 2 0 0 0 0 0 1 0
Nucleolus 27 46 19 30 41 55 88 157 88 – 87 115 7 21 20 9 7 10 28 33 27 11 31 49
Peroxisomes 0 0 0 0 0 0 1 1 0 – 0 0 1 2 2 0 0 0 0 0 0 0 0 0
SpindlePole 0 2 3 0 2 3 3 2 4 – 0 0 4 5 4 1 5 2 0 2 3 2 2 4
Vac/Vac Membrane 2 5 3 1 4 1 2 7 3 – 2 3 1 6 3 9 10 5 5 9 19 5 16 23
Unique Cell Count 128 355 434 342 473 436 591 858 406 349 374 211 302 345 269 214 196 150 382 461 177 229 268
Labelled Cell Count 150 391 503 430 553 604 812 1205 615 548 605 221 321 375 288 233 232 150 382 461 177 229 268


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 11.0 8.2 5.4 5.0 5.3 4.6 4.6 4.3 4.2 – 4.2 4.1 7.7 7.9 7.4 9.3 9.7 8.4 8.6 8.8 9.2
Std Deviation (1e-4) 2.5 1.6 1.1 1.1 1.4 1.1 1.3 1.1 1.1 – 1.3 1.1 2.3 2.5 3.0 2.1 2.6 2.2 1.7 1.7 1.8
Intensity Change (Log2) – – – -0.12 -0.02 -0.24 -0.23 -0.31 -0.36 – -0.37 -0.41 0.51 0.54 0.45 0.79 0.84 0.64 0.67 0.71 0.77


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Cytoplasm -0.6 0.2 -0.9 0.4 0 -0.8 – 0.3 -0.1 3.7 5.5 5.9 1.2 2.8 2.0
Endoplasmic Reticulum 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Mitochondria 2.4 -0.9 0 0 0 0 – 0 0 -5.5 -7.0 -6.7 -5.5 -5.5 -1.6
Nucleus 0.7 -0.1 -2.5 -4.5 -6.4 -7.5 – -5.7 -7.2 -0.7 -3.4 -3.3 -2.1 -1.9 -0.1
Nuclear Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Nucleolus 2.5 2.6 0 0 0 0 – 0 0 -0.6 1.5 0.9 -0.7 -0.7 0.4
Peroxisomes 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 5.1476 5.4193 5.0534 4.719 4.2923 5.1943 3.0219 2.8014 2.7976 1.9459 2.5038 2.512 8.4558 8.2296 8.0985 7.1354 7.6065 8.1994
Actin 0.0241 0 0.0278 0.0324 0 0.0013 0.0272 0 0.0007 0.0001 0.0054 0.0001 0.0079 0 0.0005 0 0.0002 0
Bud 0.0005 0 0.0002 0.0004 0 0.0001 0.0005 0 0.0001 0 0.002 0 0.0001 0 0.0001 0 0.0001 0
Bud Neck 0.0029 0.0001 0.0009 0.002 0.0003 0.001 0.0015 0 0.0008 0.0001 0.0014 0.0006 0.0002 0.0001 0.0004 0.0002 0.0073 0.0009
Bud Periphery 0.0007 0 0.0003 0.0006 0 0.0001 0.0015 0 0.0003 0 0.0017 0.0001 0.0002 0 0.0001 0 0.0004 0
Bud Site 0.0063 0 0.0009 0.0045 0 0.0001 0.001 0 0.0004 0 0.0035 0.0002 0.0003 0 0.0003 0 0.0011 0
Cell Periphery 0.0003 0 0.0001 0.0003 0 0 0.0004 0 0.0001 0 0.0001 0 0.0001 0 0 0 0.0002 0
Cytoplasm 0.0077 0 0.0005 0.0009 0.0001 0.0001 0.0018 0 0.0002 0.0001 0.004 0.0005 0.0003 0 0.0013 0 0.0005 0
Cytoplasmic Foci 0.0054 0 0.0008 0.006 0 0.0005 0.0045 0 0.0001 0 0.0005 0.0005 0.0011 0 0.0012 0 0.0005 0
Eisosomes 0.0002 0 0.0006 0.0014 0 0 0.0002 0 0.0001 0 0 0 0 0 0 0 0 0
Endoplasmic Reticulum 0.0075 0 0.0007 0.0014 0 0.0001 0.0012 0 0.0001 0 0.0008 0.0004 0.0004 0 0.0005 0 0.0005 0
Endosome 0.0273 0 0.0018 0.0072 0 0.0013 0.0063 0 0.0009 0 0.0017 0.0013 0.0023 0 0.0023 0 0.0047 0
Golgi 0.0041 0 0.0015 0.0059 0 0.0006 0.0032 0 0.0006 0 0.0003 0.0001 0.0006 0 0.0002 0 0.0005 0
Lipid Particles 0.0056 0 0.0017 0.0087 0 0.002 0.0031 0 0.0002 0 0.0001 0.0013 0.0036 0 0.0006 0 0.0018 0
Mitochondria 0.0034 0.0001 0.0017 0.0039 0.0001 0.0002 0.0031 0.0001 0.0062 0.0001 0.003 0.0135 0.0008 0 0.0005 0 0.0007 0
None 0.0061 0 0.0013 0.0044 0 0.0001 0.0008 0 0.0001 0 0.0003 0.0006 0.0005 0 0.0018 0 0.0003 0
Nuclear Periphery 0.0381 0.0004 0.0007 0.009 0.0015 0.0027 0.0095 0.0003 0.0003 0.0072 0.0086 0.0046 0.0014 0.0002 0.004 0.0001 0.0059 0.0002
Nucleolus 0.034 0.0316 0.0162 0.0102 0.0835 0.0849 0.0298 0.0236 0.025 0.0104 0.0742 0.0684 0.0303 0.0315 0.0314 0.0239 0.1747 0.0799
Nucleus 0.7963 0.9673 0.94 0.8902 0.9105 0.9013 0.888 0.9751 0.961 0.9813 0.888 0.9029 0.9449 0.9677 0.946 0.9755 0.7916 0.9173
Peroxisomes 0.0041 0 0.0015 0.0071 0 0.0004 0.0089 0 0.0001 0 0.0002 0.0002 0.0006 0 0.0001 0 0.0002 0
Punctate Nuclear 0.0177 0.0005 0.0008 0.0023 0.0039 0.003 0.0044 0.0008 0.0013 0.0006 0.0022 0.0033 0.0028 0.0002 0.0081 0.0002 0.0049 0.0014
Vacuole 0.0048 0 0.0001 0.0005 0 0.0001 0.0024 0 0.0008 0 0.0014 0.0006 0.0011 0 0.0004 0 0.0033 0
Vacuole Periphery 0.0028 0 0.0001 0.0004 0 0.0001 0.0009 0 0.0007 0.0001 0.0006 0.0005 0.0004 0 0.0002 0 0.0007 0

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 14.1212 15.3804 15.0737 22.6173 15.1521 13.3023 20.3925 24.2595 17.987 23.7135
Translational Efficiency 0.8147 0.7979 0.7354 0.4596 0.6741 0.7849 0.6015 0.418 0.6373 0.5041

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1745 1126 126 1115 926 1155 139 832 2671 2281 265 1947

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 795.95 941.45 1325.33 1095.46 787.46 871.06 1169.14 1083.19 793.01 905.81 1243.40 1090.22
Standard Deviation 113.52 182.53 182.25 169.57 152.36 167.81 140.95 156.51 128.38 178.73 179.72 164.23
Intensity Change Log 2 — 0.242207 0.735602 0.460787 — 0.145565 0.570169 0.460008 — 0.194954 0.655698 0.460400

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000402 0.000700 0.001848 0.001746 0.000586 0.000595 0.001742 0.001510 0.000466 0.000647 0.001792 0.001645
Bud Neck 0.004144 0.010598 0.011014 0.044313 0.004415 0.009867 0.010024 0.034206 0.004238 0.010228 0.010494 0.039994
Bud Site 0.000519 0.001236 0.001620 0.009976 0.000647 0.001789 0.001592 0.006447 0.000563 0.001516 0.001605 0.008468
Cell Periphery 0.000079 0.000104 0.000046 0.000230 0.000147 0.000182 0.000078 0.000137 0.000103 0.000144 0.000063 0.000190
Cytoplasm 0.000883 0.000810 0.000142 0.008058 0.002278 0.004949 0.000305 0.005415 0.001366 0.002906 0.000227 0.006928
Cytoplasmic Foci 0.000160 0.000259 0.000101 0.002624 0.000070 0.001808 0.004371 0.005352 0.000129 0.001043 0.002341 0.003790
Eisosomes 0.000032 0.000034 0.000091 0.000039 0.000033 0.000041 0.000067 0.000043 0.000033 0.000038 0.000078 0.000041
Endoplasmic Reticulum 0.001885 0.002413 0.003329 0.003219 0.002386 0.002665 0.004078 0.004804 0.002059 0.002540 0.003722 0.003896
Endosome 0.000107 0.000227 0.000105 0.003461 0.000104 0.001368 0.001519 0.013134 0.000106 0.000804 0.000846 0.007594
Golgi 0.000191 0.000352 0.000202 0.005798 0.000129 0.000498 0.005693 0.007548 0.000169 0.000426 0.003082 0.006545
Lipid Particles 0.000168 0.000062 0.000156 0.000219 0.000071 0.000814 0.000121 0.000956 0.000134 0.000443 0.000137 0.000534
Mitochondria 0.001477 0.001133 0.002119 0.004638 0.000821 0.003284 0.002398 0.007899 0.001250 0.002223 0.002266 0.006031
Mitotic Spindle 0.000989 0.003639 0.000665 0.060973 0.000783 0.006194 0.001454 0.034013 0.000918 0.004933 0.001079 0.049453
None 0.003414 0.003662 0.000979 0.006470 0.011109 0.015938 0.002472 0.005010 0.006081 0.009878 0.001762 0.005846
Nuclear Periphery 0.000066 0.000175 0.000172 0.002287 0.000072 0.000643 0.000231 0.003342 0.000068 0.000412 0.000203 0.002738
Nuclear Periphery Foci 0.000413 0.000381 0.000084 0.001842 0.000491 0.001038 0.000106 0.003242 0.000440 0.000713 0.000096 0.002440
Nucleolus 0.084138 0.078974 0.083789 0.022054 0.075717 0.068040 0.082324 0.019555 0.081218 0.073438 0.083021 0.020986
Nucleus 0.899919* 0.893786* 0.886666* 0.812666* 0.899043* 0.877268* 0.877484* 0.828708* 0.899615* 0.885422* 0.881850* 0.819521*
Peroxisomes 0.000572 0.000764 0.005286 0.000931 0.000753 0.001169 0.002224 0.000750 0.000635 0.000969 0.003680 0.000853
Vacuole 0.000258 0.000406 0.001336 0.005667 0.000185 0.001199 0.001420 0.011969 0.000233 0.000807 0.001380 0.008360
Vacuole Periphery 0.000185 0.000285 0.000248 0.002790 0.000160 0.000652 0.000298 0.005961 0.000176 0.000471 0.000274 0.004145

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -6.69 -11.00 -9.35 -7.45 0.88 -0.22 -9.63 -4.24 -4.18 0.87 -4.53 -14.75 -8.76 -7.63 1.32
Bud Neck -7.84 -6.97 -19.80 -16.53 -14.42 -5.45 -6.62 -17.12 -13.85 -10.26 -9.24 -9.50 -25.98 -21.46 -17.43
Bud Site -2.80 -6.99 -7.44 -6.44 -5.89 -3.57 -5.83 -7.96 -5.94 -4.25 -4.58 -8.44 -10.19 -8.40 -7.03
Cell Periphery -0.96 0.77 -2.50 -1.99 -3.37 -0.73 1.22 0.48 2.18 -1.95 -1.64 1.19 -1.84 -0.40 -3.66
Cytoplasm 0.38 2.13 -5.47 -5.85 -6.67 -2.37 2.28 -2.20 -0.20 -4.74 -2.82 3.06 -5.58 -3.46 -8.10
Cytoplasmic Foci -0.56 0.56 -2.54 -2.02 -2.85 -3.09 -0.99 -3.29 0.20 0.20 -3.06 -0.95 -4.10 -0.34 -0.02
Eisosomes -1.06 -9.52 -9.08 -7.50 6.78 -2.72 -8.70 -6.04 -2.73 5.77 -3.04 -12.93 -10.92 -6.75 8.83
Endoplasmic Reticulum -3.32 -3.37 -10.73 -7.93 -1.40 -1.21 -5.31 -6.21 -5.66 0.95 -3.72 -6.48 -12.04 -9.47 -0.11
Endosome -1.74 -1.00 -4.25 -3.82 -4.11 -2.50 -1.18 -3.52 -2.31 -2.41 -2.69 -1.23 -4.70 -3.13 -3.16
Golgi -0.84 -0.61 -2.25 -2.02 -2.20 -2.98 -1.04 -2.87 -2.40 0.21 -2.19 -1.05 -3.52 -3.05 -0.29
Lipid Particles 1.09 -0.44 -0.94 -6.33 -0.39 -2.54 -2.17 -2.16 0.92 -1.67 -1.90 -0.78 -2.15 0.47 -1.73
Mitochondria 0.66 -1.83 -5.63 -8.97 -5.89 -3.09 -3.86 -5.32 -1.69 -2.72 -1.89 -3.22 -7.33 -4.83 -5.13
Mitotic Spindle -1.79 -0.23 -8.81 -7.94 -8.80 -2.98 -1.55 -6.75 -5.27 -6.36 -3.45 -1.49 -11.09 -9.45 -10.79
None -0.19 3.58 -1.86 -1.88 -5.78 -1.61 4.46 3.08 4.69 -3.82 -2.60 5.26 1.00 3.65 -6.42
Nuclear Periphery -3.56 -3.60 -8.30 -7.90 -7.47 -3.57 -3.58 -3.27 -2.37 -2.67 -4.20 -4.85 -7.16 -5.99 -6.16
Nuclear Periphery Foci 0.33 2.20 -1.57 -1.75 -2.56 -1.43 1.88 -1.45 -0.01 -2.54 -1.34 2.86 -2.19 -0.85 -3.59
Nucleolus 1.03 -0.31 16.79 12.98 5.80 1.44 -0.97 11.85 10.19 5.09 2.19 -0.77 20.65 16.37 7.61
Nucleus 1.44 2.07 10.03 8.55 4.21 3.37 2.32 6.67 3.71 1.70 3.70 3.13 11.99 8.54 4.05
Peroxisomes -1.26 -6.27 -0.73 0.53 6.13 -1.78 -4.80 0.73 2.38 5.36 -2.32 -7.51 -0.37 2.21 7.48
Vacuole -1.12 -2.42 -7.72 -7.50 -4.06 -3.56 -4.16 -6.01 -5.21 -4.25 -3.52 -4.41 -9.45 -8.56 -5.97
Vacuole Periphery -1.48 -2.35 -2.71 -2.39 -2.42 -2.58 -2.16 -3.76 -2.64 -3.46 -2.85 -2.75 -4.60 -3.42 -4.19
Standard name
Human Ortholog
Description Component of the RNA polymerase II mediator complex; mediator is required for transcriptional activation and also has a role in basal transcription
Localization
Cell Percentages nucleus (98%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-4

Nut1

Nut1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Nut1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available