Standard name | |
---|---|
Human Ortholog | |
Description | Peripheral membrane protein required for COPI vesicle fusion to the ER; mediates Sey1p-independent homotypic ER fusion; prohibits back-fusion of COPII vesicles with the ER; forms a tethering complex with Sec39p and Dsl1p that interacts with ER SNAREs Sec20p and Use1p |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 24 | 24 | 8 | 9 | 3 | 0 | 0 | 2 | 2 | 2 | 1 | 1 | 8 | 2 | 12 | 11 | 4 | 13 | 13 | 21 | 23 | 15 | 28 | 16 |
Bud | 8 | 18 | 5 | 11 | 5 | 2 | 7 | 10 | 20 | 23 | 17 | 9 | 2 | 2 | 1 | 5 | 3 | 6 | 2 | 4 | 0 | 0 | 1 | 2 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 3 | 9 | 1 | 3 | 6 | 3 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 5 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 10 | 8 | 10 | 11 | 15 | 13 | 11 | 7 | 10 | 9 | 10 | 11 | 3 | 9 | 6 | 15 | 10 | 4 | 3 | 7 | 5 | 9 | 13 | 16 |
Cytoplasm | 52 | 78 | 48 | 73 | 117 | 122 | 108 | 151 | 151 | 153 | 112 | 142 | 30 | 136 | 143 | 87 | 63 | 45 | 16 | 28 | 4 | 7 | 13 | 27 |
Endoplasmic Reticulum | 137 | 92 | 89 | 41 | 43 | 37 | 28 | 43 | 25 | 16 | 22 | 45 | 31 | 44 | 53 | 3 | 8 | 7 | 204 | 151 | 178 | 135 | 192 | 212 |
Endosome | 3 | 0 | 7 | 10 | 16 | 8 | 12 | 10 | 7 | 5 | 5 | 7 | 0 | 0 | 0 | 0 | 7 | 3 | 2 | 5 | 3 | 1 | 2 | 3 |
Golgi | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 4 | 1 | 3 | 5 | 4 | 4 |
Mitochondria | 18 | 28 | 37 | 87 | 55 | 134 | 186 | 303 | 330 | 401 | 246 | 302 | 2 | 1 | 0 | 20 | 16 | 10 | 16 | 26 | 18 | 24 | 35 | 38 |
Nucleus | 0 | 0 | 1 | 0 | 0 | 1 | 2 | 4 | 0 | 2 | 6 | 1 | 0 | 0 | 0 | 0 | 2 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 2 | 0 | 1 | 0 | 2 | 5 | 5 | 8 | 1 | 11 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 3 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 1 | 1 | 2 | 1 | 1 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 2 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 2 | 0 | 1 | 2 | 1 | 0 | 0 | 1 | 0 | 1 | 3 | 0 | 0 | 0 | 0 | 0 | 0 |
Vac/Vac Membrane | 22 | 4 | 7 | 5 | 20 | 21 | 44 | 67 | 44 | 32 | 10 | 33 | 0 | 1 | 7 | 2 | 6 | 14 | 8 | 17 | 1 | 7 | 8 | 20 |
Unique Cell Count | 225 | 216 | 176 | 188 | 233 | 287 | 324 | 495 | 466 | 519 | 344 | 437 | 67 | 176 | 204 | 127 | 109 | 91 | 279 | 276 | 242 | 217 | 313 | 351 |
Labelled Cell Count | 275 | 252 | 217 | 248 | 277 | 341 | 401 | 606 | 594 | 660 | 434 | 566 | 76 | 195 | 224 | 144 | 123 | 107 | 279 | 276 | 242 | 217 | 313 | 351 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 6.3 | 6.2 | 6.0 | 5.5 | 5.6 | 4.6 | 4.5 | 4.5 | 4.0 | 3.8 | 3.9 | 4.0 | 6.5 | 7.0 | 7.1 | 5.1 | 6.0 | 6.8 | 7.5 | 7.4 | 8.0 |
Std Deviation (1e-4) | 0.7 | 0.8 | 1.5 | 1.4 | 2.0 | 1.4 | 1.5 | 1.3 | 1.0 | 0.9 | 0.9 | 1.0 | 0.8 | 1.0 | 1.0 | 0.9 | 1.2 | 1.6 | 1.9 | 1.2 | 1.6 |
Intensity Change (Log2) | – | – | – | -0.12 | -0.08 | -0.37 | -0.4 | -0.42 | -0.57 | -0.67 | -0.61 | -0.58 | 0.12 | 0.24 | 0.24 | -0.22 | 0.02 | 0.19 | 0.32 | 0.32 | 0.42 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0.1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | -1.9 | 0.6 | 1.5 | 0 | 2.8 |
Bud | 1.4 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0.1 | 0.3 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | -0.2 | -1.3 | 1.9 | 1.1 | 0 |
Cytoplasm | 2.3 | 4.7 | 3.3 | 1.4 | 0.8 | 1.3 | 0.6 | 1.2 | 1.3 | 2.6 | 9.4 | 8.3 | 7.1 | 5.1 | 3.6 |
Endoplasmic Reticulum | -5.7 | -6.9 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | -0.6 | -4.9 | -4.9 | -9.0 | -7.5 | -6.9 |
Endosome | 0.6 | 1.3 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0.9 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 5.1 | 0.6 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | -3.4 | -6.2 | -6.9 | -1.2 | -1.3 | -2.0 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | -0.7 | 1.9 | 1.5 | 3.4 | 3.5 | 2.3 | 1.1 | -0.6 | 1.6 | 0 | 0 | -0.3 | 0 | 0 | 3.3 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 1.5617 | 1.9241 | 1.3847 | 1.7109 | 1.0985 | 1.6165 | 2.2203 | 3.3634 | 3.1996 | 2.8263 | 2.8733 | 3.1569 | 2.2826 | 2.7229 | 2.5559 | 2.5722 | 2.5345 | 2.6324 |
Actin | 0.0302 | 0.0062 | 0.0203 | 0.0167 | 0.0112 | 0.016 | 0.0113 | 0.0291 | 0.0309 | 0.0369 | 0.0235 | 0.0309 | 0.0237 | 0.0107 | 0.0508 | 0.0105 | 0.0057 | 0.0217 |
Bud | 0.001 | 0.0022 | 0.0067 | 0.0056 | 0.0006 | 0.0017 | 0.0007 | 0.0007 | 0.0052 | 0.0016 | 0.0017 | 0.0004 | 0.0003 | 0.0004 | 0.0016 | 0.0007 | 0.0002 | 0.0003 |
Bud Neck | 0.0064 | 0.0017 | 0.0062 | 0.0317 | 0.0012 | 0.0155 | 0.0028 | 0.0027 | 0.0075 | 0.0026 | 0.0047 | 0.0343 | 0.0031 | 0.0013 | 0.005 | 0.0023 | 0.002 | 0.0277 |
Bud Periphery | 0.003 | 0.0066 | 0.0176 | 0.0381 | 0.0033 | 0.0021 | 0.0018 | 0.0012 | 0.0204 | 0.0051 | 0.0038 | 0.0018 | 0.0005 | 0.0007 | 0.0026 | 0.0041 | 0.0009 | 0.001 |
Bud Site | 0.0056 | 0.0395 | 0.0241 | 0.0021 | 0.0008 | 0.0013 | 0.0194 | 0.0553 | 0.0089 | 0.002 | 0.0033 | 0.0007 | 0.0073 | 0.0202 | 0.0145 | 0.0018 | 0.0004 | 0.0008 |
Cell Periphery | 0.0065 | 0.0054 | 0.0046 | 0.0054 | 0.002 | 0.0026 | 0.0092 | 0.0173 | 0.0149 | 0.0065 | 0.0023 | 0.0055 | 0.0053 | 0.0068 | 0.0061 | 0.0162 | 0.0051 | 0.0058 |
Cytoplasm | 0.3094 | 0.4994 | 0.4097 | 0.332 | 0.4759 | 0.4417 | 0.3034 | 0.4524 | 0.3696 | 0.2775 | 0.32 | 0.4413 | 0.2762 | 0.3702 | 0.4175 | 0.379 | 0.3853 | 0.4389 |
Cytoplasmic Foci | 0.0318 | 0.0228 | 0.0256 | 0.0257 | 0.0218 | 0.0323 | 0.0213 | 0.0343 | 0.0305 | 0.0289 | 0.0427 | 0.0286 | 0.0238 | 0.0286 | 0.0241 | 0.0088 | 0.0109 | 0.0189 |
Eisosomes | 0.0008 | 0.0005 | 0.0011 | 0.0005 | 0.0006 | 0.0004 | 0.0009 | 0.0012 | 0.0011 | 0.0006 | 0.0003 | 0.0005 | 0.0008 | 0.0005 | 0.0005 | 0.0008 | 0.0015 | 0.0005 |
Endoplasmic Reticulum | 0.3051 | 0.1691 | 0.2466 | 0.3702 | 0.2438 | 0.2329 | 0.4679 | 0.2405 | 0.2828 | 0.3234 | 0.3399 | 0.2905 | 0.5058 | 0.3265 | 0.3717 | 0.4217 | 0.4037 | 0.3711 |
Endosome | 0.0471 | 0.0315 | 0.0365 | 0.0286 | 0.0283 | 0.0484 | 0.0262 | 0.0431 | 0.0492 | 0.0647 | 0.0852 | 0.0268 | 0.0214 | 0.056 | 0.0235 | 0.0125 | 0.0177 | 0.0323 |
Golgi | 0.01 | 0.0068 | 0.01 | 0.0103 | 0.0069 | 0.0106 | 0.0084 | 0.0134 | 0.0228 | 0.0313 | 0.0269 | 0.0085 | 0.0136 | 0.0102 | 0.0134 | 0.0075 | 0.0082 | 0.0123 |
Lipid Particles | 0.029 | 0.0137 | 0.0117 | 0.01 | 0.0058 | 0.0077 | 0.027 | 0.0209 | 0.0365 | 0.0075 | 0.0155 | 0.0133 | 0.03 | 0.0227 | 0.0244 | 0.0138 | 0.0035 | 0.0098 |
Mitochondria | 0.0059 | 0.0103 | 0.0058 | 0.0067 | 0.0053 | 0.011 | 0.0022 | 0.0015 | 0.0102 | 0.0585 | 0.0177 | 0.0036 | 0.0014 | 0.0023 | 0.0025 | 0.0059 | 0.0038 | 0.0054 |
None | 0.1635 | 0.1607 | 0.1452 | 0.0748 | 0.1727 | 0.1503 | 0.0711 | 0.0688 | 0.0791 | 0.1285 | 0.0675 | 0.1002 | 0.0504 | 0.101 | 0.0277 | 0.1005 | 0.141 | 0.0437 |
Nuclear Periphery | 0.0197 | 0.0062 | 0.0091 | 0.0085 | 0.0069 | 0.0089 | 0.0082 | 0.0045 | 0.009 | 0.0103 | 0.0046 | 0.0039 | 0.008 | 0.0203 | 0.0058 | 0.0056 | 0.0034 | 0.0036 |
Nucleolus | 0.0006 | 0.0008 | 0.0013 | 0.0002 | 0.0004 | 0.0006 | 0.0009 | 0.0004 | 0.0003 | 0.0002 | 0.0004 | 0.0001 | 0.0006 | 0.0012 | 0.0002 | 0.0001 | 0.0002 | 0.0001 |
Nucleus | 0.0062 | 0.0035 | 0.0044 | 0.0022 | 0.0047 | 0.0038 | 0.0025 | 0.0019 | 0.0019 | 0.0024 | 0.0015 | 0.0013 | 0.0015 | 0.0036 | 0.0012 | 0.0013 | 0.0014 | 0.001 |
Peroxisomes | 0.0051 | 0.0005 | 0.0012 | 0.0187 | 0.0004 | 0.0016 | 0.0025 | 0.0014 | 0.0086 | 0.0013 | 0.0242 | 0.0021 | 0.0077 | 0.0007 | 0.0015 | 0.0002 | 0.0002 | 0.0004 |
Punctate Nuclear | 0.0041 | 0.0029 | 0.0035 | 0.0033 | 0.0016 | 0.004 | 0.0026 | 0.0013 | 0.0013 | 0.0013 | 0.0008 | 0.0007 | 0.0136 | 0.0073 | 0.0008 | 0.0005 | 0.0005 | 0.0006 |
Vacuole | 0.0074 | 0.0087 | 0.0075 | 0.0078 | 0.005 | 0.0051 | 0.0089 | 0.0075 | 0.0073 | 0.0066 | 0.0113 | 0.0042 | 0.0046 | 0.0075 | 0.0041 | 0.0053 | 0.0039 | 0.0035 |
Vacuole Periphery | 0.0014 | 0.001 | 0.0014 | 0.0011 | 0.0007 | 0.0016 | 0.0008 | 0.0005 | 0.0019 | 0.002 | 0.0022 | 0.0006 | 0.0006 | 0.001 | 0.0005 | 0.0008 | 0.0006 | 0.0007 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 15.1941 | 14.9877 | 17.3773 | 21.5349 | 18.0015 | 8.3783 | 21.3103 | 20.1363 | 15.9333 | 21.114 |
Translational Efficiency | 1.243 | 1.1738 | 0.907 | 0.5722 | 0.7619 | 1.8602 | 0.8492 | 0.7027 | 0.89 | 0.7586 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
188 | 475 | 1342 | 1177 | 1804 | 1312 | 1996 | 1662 | 1992 | 1787 | 3338 | 2839 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 1021.99 | 801.45 | 935.45 | 1020.90 | 783.99 | 822.04 | 956.41 | 1014.47 | 806.45 | 816.57 | 947.98 | 1017.14 |
Standard Deviation | 132.90 | 108.36 | 149.04 | 162.70 | 100.29 | 105.49 | 115.36 | 128.09 | 124.97 | 106.65 | 130.36 | 143.49 |
Intensity Change Log 2 | — | -0.350697 | -0.127649 | -0.001540 | — | 0.068373 | 0.286794 | 0.371819 | — | -0.153683 | 0.067023 | 0.172509 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.014653 | 0.003354 | 0.007603 | 0.055917 | 0.002064 | 0.001620 | 0.015668 | 0.019486 | 0.003252 | 0.002081 | 0.012425 | 0.034590 |
Bud Neck | 0.012821 | 0.009914 | 0.011579 | 0.024702 | 0.006390 | 0.019650 | 0.031837 | 0.026391 | 0.006997 | 0.017062 | 0.023693 | 0.025691 |
Bud Site | 0.065016 | 0.025124 | 0.041165 | 0.107208* | 0.005579 | 0.018850 | 0.043697 | 0.077781 | 0.011189 | 0.020518 | 0.042679 | 0.089981 |
Cell Periphery | 0.002439 | 0.001486 | 0.002289 | 0.017490 | 0.001233 | 0.002077 | 0.002865 | 0.002693 | 0.001347 | 0.001920 | 0.002633 | 0.008827 |
Cytoplasm | 0.162980* | 0.157977* | 0.342324* | 0.230266* | 0.265247* | 0.237837* | 0.330066* | 0.263078* | 0.255596* | 0.216610* | 0.334994* | 0.249475* |
Cytoplasmic Foci | 0.122329 | 0.222540* | 0.091833 | 0.047240 | 0.135650 | 0.161200 | 0.048933 | 0.107006 | 0.134393 | 0.177505 | 0.066180 | 0.082228 |
Eisosomes | 0.001571 | 0.001252 | 0.000748 | 0.010269 | 0.002715 | 0.001534 | 0.003420 | 0.003007 | 0.002607 | 0.001459 | 0.002346 | 0.006017 |
Endoplasmic Reticulum | 0.125003* | 0.239429* | 0.220633* | 0.236586* | 0.279353* | 0.171072* | 0.202983* | 0.105521* | 0.264785* | 0.189242* | 0.210079* | 0.159858* |
Endosome | 0.035509 | 0.054648 | 0.017104 | 0.013898 | 0.017062 | 0.046559 | 0.013518 | 0.032037 | 0.018803 | 0.048709 | 0.014960 | 0.024517 |
Golgi | 0.015179 | 0.056025 | 0.005419 | 0.012186 | 0.068491 | 0.040589 | 0.007654 | 0.012546 | 0.063460 | 0.044692 | 0.006756 | 0.012397 |
Lipid Particles | 0.050469 | 0.091918 | 0.032027 | 0.071817 | 0.120722* | 0.136064* | 0.047696 | 0.064701 | 0.114092* | 0.124330* | 0.041397 | 0.067651 |
Mitochondria | 0.007083 | 0.018114 | 0.001132 | 0.002583 | 0.022448 | 0.036702 | 0.004617 | 0.006874 | 0.020998 | 0.031761 | 0.003216 | 0.005095 |
Mitotic Spindle | 0.005576 | 0.001105 | 0.002807 | 0.003284 | 0.000427 | 0.001569 | 0.003055 | 0.016617 | 0.000913 | 0.001446 | 0.002955 | 0.011089 |
None | 0.000052 | 0.000929 | 0.002314 | 0.000744 | 0.004603 | 0.002633 | 0.001639 | 0.000903 | 0.004174 | 0.002180 | 0.001911 | 0.000837 |
Nuclear Periphery | 0.000804 | 0.004523 | 0.002139 | 0.000336 | 0.001526 | 0.004521 | 0.001725 | 0.002041 | 0.001458 | 0.004521 | 0.001891 | 0.001334 |
Nuclear Periphery Foci | 0.023487 | 0.009699 | 0.037374 | 0.012603 | 0.002347 | 0.004256 | 0.009917 | 0.013422 | 0.004342 | 0.005703 | 0.020956 | 0.013083 |
Nucleolus | 0.000103 | 0.000565 | 0.000763 | 0.000172 | 0.000730 | 0.001268 | 0.000483 | 0.000452 | 0.000671 | 0.001081 | 0.000595 | 0.000336 |
Nucleus | 0.020217 | 0.015412 | 0.101257 | 0.041569 | 0.026078 | 0.029954 | 0.127613 | 0.049728 | 0.025525 | 0.026089 | 0.117017 | 0.046345 |
Peroxisomes | 0.004055 | 0.010391 | 0.002133 | 0.006963 | 0.011197 | 0.012436 | 0.007536 | 0.009942 | 0.010523 | 0.011893 | 0.005364 | 0.008707 |
Vacuole | 0.325359* | 0.060817 | 0.076426 | 0.103112 | 0.022077 | 0.059885 | 0.093928 | 0.183439 | 0.050700 | 0.060133 | 0.086892 | 0.150137 |
Vacuole Periphery | 0.005296 | 0.014779 | 0.000932 | 0.001054 | 0.004059 | 0.009721 | 0.001149 | 0.002336 | 0.004176 | 0.011066 | 0.001062 | 0.001804 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | 3.37 | 1.95 | -9.20 | -16.11 | -14.30 | 0.71 | -9.15 | -10.67 | -11.26 | -1.32 | 1.77 | -8.61 | -17.94 | -19.35 | -11.48 |
Bud Neck | 0.71 | 0.05 | -4.65 | -7.21 | -7.42 | -7.13 | -13.34 | -12.06 | -2.43 | 2.96 | -6.83 | -12.67 | -14.83 | -4.67 | -0.81 |
Bud Site | 3.22 | 1.79 | -3.90 | -12.90 | -10.87 | -6.62 | -14.01 | -17.75 | -12.90 | -6.53 | -4.72 | -13.75 | -22.99 | -18.85 | -12.12 |
Cell Periphery | 1.76 | -0.01 | -11.99 | -12.83 | -12.33 | -3.15 | -7.65 | -3.65 | -0.91 | 0.97 | -2.70 | -7.77 | -13.09 | -11.38 | -10.54 |
Cytoplasm | 0.21 | -12.03 | -4.61 | -7.16 | 12.11 | 3.54 | -9.04 | 0.71 | -2.62 | 8.88 | 5.74 | -12.71 | 1.32 | -4.65 | 14.57 |
Cytoplasmic Foci | -8.58 | 2.76 | 7.83 | 18.91 | 11.53 | -4.78 | 22.81 | 6.44 | 10.42 | -15.96 | -8.64 | 19.00 | 13.72 | 21.09 | -5.76 |
Eisosomes | 0.69 | 3.18 | -10.79 | -11.04 | -12.22 | 3.37 | -1.46 | 0.02 | -4.19 | 1.72 | 3.57 | 0.93 | -6.63 | -11.55 | -8.86 |
Endoplasmic Reticulum | -6.20 | -6.20 | -6.84 | 0.63 | -1.16 | 10.98 | 8.82 | 21.64 | 8.80 | 13.42 | 8.23 | 7.25 | 14.18 | 4.87 | 8.46 |
Endosome | -4.19 | 3.82 | 4.64 | 11.78 | 2.75 | -13.35 | 3.76 | -8.92 | 6.37 | -11.12 | -15.71 | 4.07 | -4.25 | 12.54 | -8.00 |
Golgi | -6.70 | 1.74 | 0.40 | 9.91 | -5.40 | 8.75 | 26.43 | 23.58 | 11.99 | -3.46 | 6.37 | 26.43 | 23.32 | 15.48 | -5.99 |
Lipid Particles | -5.40 | 2.65 | -3.55 | 2.94 | -9.95 | -2.59 | 18.44 | 13.19 | 13.33 | -4.04 | -2.08 | 20.97 | 12.32 | 13.05 | -9.24 |
Mitochondria | -2.80 | 1.40 | 1.05 | 6.69 | -2.98 | -5.03 | 12.22 | 9.26 | 11.57 | -1.54 | -4.64 | 13.05 | 11.03 | 13.60 | -2.20 |
Mitotic Spindle | 1.76 | 0.78 | 0.77 | -1.86 | 0.06 | -2.49 | -3.43 | -7.73 | -6.97 | -6.12 | -1.51 | -3.95 | -7.68 | -7.03 | -5.67 |
None | -3.06 | -4.24 | -4.16 | 0.63 | 2.84 | 2.34 | 4.04 | 4.99 | 3.78 | 2.34 | 2.73 | 3.31 | 5.07 | 4.00 | 3.69 |
Nuclear Periphery | -2.74 | -6.17 | 3.80 | 3.07 | 9.30 | -5.31 | -0.90 | -1.80 | 3.60 | -1.17 | -5.50 | -2.21 | 0.03 | 5.38 | 1.94 |
Nuclear Periphery Foci | 3.75 | -3.94 | 3.03 | -1.92 | 11.47 | -3.49 | -11.96 | -12.90 | -9.41 | -3.47 | -1.97 | -17.28 | -13.11 | -9.28 | 6.92 |
Nucleolus | -3.82 | -3.25 | -3.58 | 3.23 | 2.94 | -1.32 | 0.90 | 0.88 | 3.60 | -0.10 | -1.16 | 0.25 | 1.20 | 4.46 | 2.81 |
Nucleus | 1.63 | -18.66 | -6.90 | -8.88 | 13.37 | -2.00 | -27.77 | -10.00 | -7.83 | 18.39 | -0.35 | -33.11 | -11.54 | -10.59 | 22.73 |
Peroxisomes | -6.68 | 3.12 | -3.92 | 3.71 | -8.09 | -1.36 | 4.94 | 2.06 | 3.05 | -2.44 | -1.79 | 8.55 | 3.28 | 4.87 | -5.46 |
Vacuole | 13.83 | 13.13 | 11.33 | -8.34 | -6.52 | -11.83 | -23.08 | -33.20 | -23.89 | -16.96 | -1.56 | -10.08 | -23.50 | -24.05 | -17.46 |
Vacuole Periphery | -3.40 | 1.73 | 1.69 | 6.78 | -0.29 | -8.40 | 7.01 | 4.09 | 12.05 | -2.73 | -8.83 | 7.41 | 5.57 | 13.36 | -2.53 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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