Standard name
Human Ortholog
Description Subunit of ATP-dependent Isw2p-Itc1p chromatin remodeling complex; required for repression of a-specific genes, repression of early meiotic genes during mitotic growth, and repression of INO1; similar to mammalian Acf1p, the regulatory subunit of the mammalian ATP-utilizing chromatin assembly and modifying factor (ACF) complex; ITC1 has a paralog, YPL216W, that arose from the whole genome duplication
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0.05 0.05 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0.12 0.15 0.09 0.12 0.11 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0.06 0.3 0.43 0.33 0.52 0.52 0.52 0.61 0.61 0.54 0.6 0 0 0 0 0 0.07 0 0 0 0 0 0
Nucleus 0.95 0.88 0.92 0.9 0.86 0.85 0.83 0.81 0.69 0.67 0.77 0.7 0.9 0.85 0.8 0.83 0.79 0.79 0.93 0.88 0.93 0.87 0.69 0.62
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.07 0.06 0.07 0.05 0.06 0.07 0.12 0.16 0.13 0.16 0.19 0.22 0.14 0.09 0.11 0.05 0.06 0 0 0 0 0 0.11 0.16
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0.12 0.17
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0
Bud 0 1 2 2 0 3 0 1 2 1 4 10 0 0 0 0 0 0 1 1 1 0 1 0
Bud Neck 0 0 0 0 0 0 0 0 1 0 1 0 0 0 0 0 0 0 0 0 0 0 1 0
Bud Site 0 1 0 0 3 8 20 19 4 11 12 11 0 0 2 0 1 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 4 7 2 4 10 5 7 13 5 2 4 7 8 23 32 30 21 28 0 5 0 0 1 1
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 5 5 5 0 0 0 0 0 0
Endosome 2 1 0 0 0 0 0 0 0 0 0 0 0 1 0 4 3 1 1 0 0 0 1 1
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 2 1 1 1 1 1 1 1
Mitochondria 2 14 67 65 75 150 200 199 141 154 165 193 4 1 8 8 1 17 1 1 0 1 2 3
Nucleus 361 220 207 137 197 248 321 311 160 168 234 225 112 161 168 282 139 195 363 234 213 115 142 163
Nuclear Periphery 4 3 1 1 2 5 5 9 5 9 10 9 0 0 2 0 1 0 0 0 0 0 0 0
Nucleolus 25 15 15 8 14 21 45 61 30 40 58 72 17 17 23 17 10 9 13 5 3 4 23 41
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 1 0 0 0 0 0 0 0
SpindlePole 5 0 3 0 1 2 0 2 0 0 2 1 0 1 3 2 5 0 2 1 0 0 1 1
Vac/Vac Membrane 5 8 0 1 3 0 2 0 1 1 1 0 0 1 0 12 6 9 6 12 6 5 25 45
Unique Cell Count 379 250 226 152 230 291 388 386 231 252 304 323 125 189 211 340 177 247 393 266 229 132 207 264
Labelled Cell Count 408 270 297 218 305 442 600 615 349 386 491 528 141 205 239 364 195 265 393 266 229 132 207 264


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 8.6 7.0 4.7 4.4 4.8 4.2 4.1 4.2 3.8 3.9 3.8 3.8 7.2 7.6 6.4 9.8 11.2 10.6 6.9 8.2 8.3
Std Deviation (1e-4) 1.8 1.5 0.9 0.9 1.3 1.0 0.9 1.1 1.0 0.9 1.0 1.1 1.6 1.8 1.7 2.3 3.3 2.4 1.4 1.9 1.3
Intensity Change (Log2) – – – -0.1 0.03 -0.18 -0.18 -0.15 -0.3 -0.28 -0.29 -0.32 0.62 0.7 0.46 1.07 1.25 1.18 0.55 0.8 0.82


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 2.3 0 0 1.9 0 0 0 0 0 4.8 5.6 4.0 4.7 4.7
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 -5.9 -8.0 -7.2 -9.4 -7.7 -6.5
Nucleus -0.5 -2.0 -2.2 -3.1 -3.7 -6.0 -6.6 -4.5 -6.2 -0.6 -2.1 -3.6 -2.9 -3.7 -3.8
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 2.2 0.9 1.6 -0.8 -0.4 -1.5
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 3.1367 3.3599 3.0747 3.0023 2.8625 3.5724 5.4259 5.2485 5.2421 5.1577 4.4588 5.1172 6.264 6.4816 5.8407 5.9159 5.6472 5.6752
Actin 0 0 0 0 0 0 0.0094 0 0.0117 0.008 0.0125 0.0005 0.0002 0 0.0011 0 0 0.0013
Bud 0 0 0 0 0 0 0.0001 0 0.0001 0.0014 0.0003 0.0001 0 0 0.0001 0 0.001 0
Bud Neck 0.0001 0 0 0.0001 0.0002 0.0009 0.0004 0 0.0017 0.001 0.0006 0.0004 0.0001 0 0.0002 0 0.0003 0.0006
Bud Periphery 0 0 0 0 0 0 0.0003 0 0.0002 0.0029 0.0007 0.0002 0.0001 0 0.0001 0 0.0008 0
Bud Site 0 0 0 0 0 0.0001 0.0007 0 0.0061 0.0077 0.0014 0.0001 0.0001 0 0.0003 0 0.0001 0
Cell Periphery 0 0 0 0 0 0 0.0001 0 0.0001 0.0009 0.0002 0.0001 0 0 0 0 0.0001 0
Cytoplasm 0.0001 0.0001 0 0.0001 0.0002 0 0.0103 0.0001 0.0004 0.0341 0.0007 0.0003 0.0001 0 0.0002 0 0.0029 0.0001
Cytoplasmic Foci 0 0 0 0 0 0 0.0126 0 0.0079 0.0048 0.0225 0.0015 0.0001 0 0.0034 0 0.0003 0.0001
Eisosomes 0 0 0 0 0 0 0.0001 0 0.0001 0.0003 0.0001 0 0 0 0 0 0 0.0001
Endoplasmic Reticulum 0 0 0 0 0 0 0.0057 0 0.0004 0.0055 0.0007 0.0006 0.0002 0 0.0004 0 0 0.0004
Endosome 0 0 0 0 0.0001 0 0.0145 0 0.0037 0.0236 0.0311 0.0065 0.0004 0 0.0018 0 0 0.0011
Golgi 0 0 0 0 0 0 0.0036 0 0.0023 0.0056 0.0218 0.002 0.0001 0 0.0006 0 0 0.0003
Lipid Particles 0 0 0 0 0 0 0.0113 0 0.0096 0.0284 0.0479 0.003 0.0003 0 0.0025 0 0 0.0004
Mitochondria 0.0003 0.0001 0.0001 0.0001 0.0002 0.0001 0.0098 0.0001 0.0013 0.0234 0.018 0.0019 0.0004 0 0.0007 0.0001 0.011 0.0002
None 0.0001 0 0 0 0.0001 0 0.0061 0 0.0005 0.0383 0.0007 0.0001 0.0001 0 0.0004 0 0.0021 0.0004
Nuclear Periphery 0.0008 0.0007 0.0003 0.0007 0.0046 0.0001 0.0217 0.0006 0.0048 0.0226 0.0018 0.0043 0.0036 0.0002 0.0032 0.0009 0.0008 0.0024
Nucleolus 0.0235 0.0152 0.0109 0.0048 0.0414 0.0692 0.0072 0.0117 0.0053 0.008 0.0463 0.0223 0.0086 0.0162 0.0075 0.0233 0.0653 0.034
Nucleus 0.9742 0.983 0.9802 0.9931 0.9494 0.929 0.8586 0.9874 0.9298 0.7703 0.7428 0.9507 0.9834 0.9828 0.9701 0.9737 0.9125 0.9574
Peroxisomes 0 0 0 0 0 0 0.0062 0 0.0075 0.0017 0.0449 0.0001 0 0 0.0016 0 0.0001 0
Punctate Nuclear 0.0007 0.0008 0.0084 0.0009 0.0036 0.0004 0.0185 0.0001 0.0058 0.0027 0.0027 0.0046 0.0018 0.0006 0.0055 0.0019 0.0012 0.001
Vacuole 0.0001 0.0001 0 0 0.0001 0.0001 0.0016 0 0.0003 0.005 0.0015 0.0004 0.0002 0 0.0002 0 0.0007 0.0001
Vacuole Periphery 0 0 0 0 0.0001 0 0.0012 0 0.0003 0.0038 0.0009 0.0004 0.0002 0 0.0002 0 0.0005 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 10.3217 10.9164 8.2933 12.0028 13.571 12.786 12.8772 13.569 13.3325 15.1641
Translational Efficiency 0.8759 0.7147 0.849 0.6526 0.5633 0.6057 0.5796 0.523 0.4987 0.5078

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1731 91 844 346 2151 1792 1607 2691 3882 1883 2451 3037

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 720.19 1014.77 1096.71 1120.33 849.54 909.51 1052.40 1131.83 791.86 914.60 1067.66 1130.52
Standard Deviation 105.72 148.80 201.14 176.19 124.64 138.81 156.09 179.02 133.14 141.13 174.21 178.73
Intensity Change Log 2 — 0.494703 0.606733 0.637474 — 0.098408 0.308929 0.413904 — 0.293802 0.453223 0.520793

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000273 0.000534 0.000781 0.001283 0.000314 0.000530 0.000652 0.000768 0.000296 0.000530 0.000697 0.000827
Bud Neck 0.002870 0.019212 0.015410 0.025513 0.007313 0.009148 0.013935 0.029392 0.005332 0.009634 0.014443 0.028950
Bud Site 0.000997 0.003232 0.000968 0.007583 0.000525 0.002158 0.000852 0.002729 0.000735 0.002210 0.000892 0.003282
Cell Periphery 0.000105 0.000067 0.000035 0.000272 0.000121 0.000148 0.000061 0.000085 0.000114 0.000144 0.000052 0.000106
Cytoplasm 0.006602 0.000186 0.001425 0.002833 0.000206 0.000690 0.000539 0.000873 0.003058 0.000666 0.000844 0.001096
Cytoplasmic Foci 0.000291 0.000050 0.000427 0.001517 0.000054 0.000818 0.000020 0.000302 0.000160 0.000781 0.000160 0.000441
Eisosomes 0.000018 0.000062 0.000044 0.000044 0.000041 0.000043 0.000052 0.000039 0.000031 0.000044 0.000049 0.000039
Endoplasmic Reticulum 0.002199 0.004675 0.001606 0.002429 0.003302 0.004245 0.002498 0.002097 0.002810 0.004266 0.002191 0.002135
Endosome 0.000190 0.000146 0.000140 0.003237 0.000138 0.000818 0.000075 0.000338 0.000161 0.000785 0.000097 0.000668
Golgi 0.000118 0.000104 0.000111 0.000514 0.000099 0.000779 0.000058 0.000233 0.000108 0.000747 0.000077 0.000265
Lipid Particles 0.000044 0.000047 0.000394 0.000086 0.000101 0.000426 0.000028 0.000112 0.000076 0.000408 0.000154 0.000109
Mitochondria 0.000349 0.000966 0.001338 0.004778 0.001099 0.002300 0.000965 0.001740 0.000765 0.002235 0.001093 0.002086
Mitotic Spindle 0.000036 0.000054 0.000739 0.019541 0.001643 0.006165 0.000608 0.004057 0.000927 0.005870 0.000653 0.005821
None 0.015515 0.004587 0.001277 0.004921 0.001084 0.002248 0.002878 0.001609 0.007519 0.002361 0.002326 0.001986
Nuclear Periphery 0.000060 0.000083 0.000095 0.000606 0.000158 0.000287 0.000122 0.001018 0.000114 0.000278 0.000113 0.000971
Nuclear Periphery Foci 0.000295 0.000029 0.000203 0.000273 0.000142 0.000464 0.000042 0.000563 0.000210 0.000443 0.000097 0.000530
Nucleolus 0.028232 0.017258 0.028665 0.018541 0.023427 0.028241 0.021512 0.011310 0.025570 0.027710 0.023975 0.012134
Nucleus 0.941186* 0.946694* 0.944825* 0.885500* 0.959425* 0.937479* 0.954223* 0.941628* 0.951292* 0.937925* 0.950987* 0.935233*
Peroxisomes 0.000190 0.000582 0.000785 0.000970 0.000236 0.000769 0.000410 0.000150 0.000215 0.000760 0.000539 0.000243
Vacuole 0.000308 0.001249 0.000558 0.010874 0.000272 0.001456 0.000382 0.000614 0.000288 0.001446 0.000443 0.001783
Vacuole Periphery 0.000121 0.000184 0.000175 0.008686 0.000299 0.000787 0.000088 0.000343 0.000220 0.000758 0.000118 0.001293

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -4.90 -10.61 -10.37 -8.17 -6.10 -3.14 -8.84 -2.80 -1.48 -0.42 -4.03 -14.19 -4.03 -2.33 -0.94
Bud Neck -3.05 -13.56 -10.67 -2.17 -5.40 -2.60 -9.21 -19.81 -17.82 -12.90 -6.48 -16.05 -23.97 -18.33 -13.94
Bud Site -1.14 -0.10 -3.46 -1.41 -3.48 -2.47 -5.77 -5.92 -0.80 -4.80 -2.25 -1.97 -6.19 -1.34 -5.79
Cell Periphery 1.31 5.61 -1.17 -1.64 -2.21 -0.58 1.98 1.29 2.07 -2.42 -0.82 3.40 1.09 1.62 -3.89
Cytoplasm 5.52 3.92 3.15 -3.01 -1.03 -2.15 -1.98 -5.43 -1.27 -2.65 4.33 3.95 3.57 -2.01 -1.26
Cytoplasmic Foci 2.13 -0.06 -0.96 -1.95 -0.79 -2.59 1.30 -1.19 1.77 -1.47 -2.00 0.43 -0.59 1.55 -0.86
Eisosomes -1.58 -16.28 -8.44 0.22 -2.14 -0.50 -4.26 1.18 1.74 8.29 -4.12 -10.96 -5.10 1.29 6.83
Endoplasmic Reticulum -2.57 4.00 -3.91 1.21 -5.84 -2.91 4.13 7.14 6.78 3.74 -4.86 4.68 4.14 6.58 -0.70
Endosome 0.67 0.57 -2.25 -2.34 -2.33 -2.96 2.31 -2.29 2.22 -3.30 -2.82 1.54 -2.90 1.48 -3.56
Golgi 0.39 0.24 -3.17 -2.86 -3.23 -2.25 0.67 -1.27 1.82 -1.89 -2.22 0.90 -1.66 1.73 -2.16
Lipid Particles -0.01 -1.00 -3.10 -2.88 0.65 -1.81 1.13 0.14 1.89 -0.99 -2.04 -0.59 -0.33 1.85 0.38
Mitochondria -2.54 -14.13 -5.10 -4.04 -3.60 -2.05 0.16 -2.41 0.68 -5.75 -2.96 -2.17 -6.04 0.09 -6.36
Mitotic Spindle -0.26 -1.60 -3.81 -3.81 -3.61 -3.03 1.14 -3.65 0.29 -4.66 -3.59 -0.07 -5.84 -0.94 -5.82
None 2.77 8.25 6.29 0.02 -3.73 -2.01 -3.60 -2.79 0.77 1.80 5.46 6.57 6.99 0.48 0.25
Nuclear Periphery -1.42 -3.31 -8.81 -8.48 -8.27 -2.71 0.40 -3.90 -3.19 -3.98 -3.80 -1.13 -4.59 -3.59 -4.50
Nuclear Periphery Foci 3.20 0.89 -0.07 -2.27 -0.80 -2.02 1.52 -1.38 0.26 -1.89 -1.45 1.87 -1.01 0.23 -1.73
Nucleolus 2.81 -1.14 0.45 -1.72 1.09 -2.92 0.41 5.44 7.76 4.49 -1.57 -0.07 6.76 6.89 5.94
Nucleus -0.67 -0.01 5.44 4.62 5.42 6.08 3.26 9.15 1.28 6.62 3.85 1.46 8.37 2.89 7.43
Peroxisomes -2.21 -10.06 -4.26 -2.28 -1.57 -3.09 -4.29 1.24 3.46 8.31 -3.34 -9.84 -2.19 2.80 6.64
Vacuole -1.19 -1.38 -3.03 -2.51 -2.83 -3.16 -1.85 -3.80 1.85 -2.04 -3.23 -2.16 -4.23 -0.06 -3.10
Vacuole Periphery -0.76 -2.79 -1.93 -1.90 -1.90 -2.15 1.89 0.01 2.35 -3.56 -2.68 1.59 -2.05 0.07 -2.50
Standard name
Human Ortholog
Description Subunit of ATP-dependent Isw2p-Itc1p chromatin remodeling complex; required for repression of a-specific genes, repression of early meiotic genes during mitotic growth, and repression of INO1; similar to mammalian Acf1p, the regulatory subunit of the mammalian ATP-utilizing chromatin assembly and modifying factor (ACF) complex; ITC1 has a paralog, YPL216W, that arose from the whole genome duplication
Localization
Cell Percentages nucleus (100%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-4

Itc1

Itc1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Itc1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available