Standard name | |
---|---|
Human Ortholog | |
Description | Subunit of a heterodimeric guanine nucleotide exchange factor (GEF); subunit of the Mon1-Ccz1 GEF complex which stimulates nucleotide exchange and activation of Ypt7p, a Rab family GTPase involved in membrane tethering and fusion events at the late endosome and vacuole; GEF activity is stimulated by membrane association and anionic phospholipids; role in localizing Ypt7p to the vacuolar membrane; required for autophagy, the CVT pathway and mitophagy; potential Cdc28 substrate |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | – | 0 | 3 | 1 | 0 | 0 | 0 |
Bud | 0 | 0 | 2 | 0 | 0 | 0 | 1 | 4 | 1 | 3 | 2 | 4 | 0 | 0 | 0 | 1 | 1 | – | 0 | 1 | 2 | 0 | 1 | 3 |
Bud Neck | 0 | 4 | 4 | 1 | 0 | 3 | 7 | 9 | 2 | 4 | 1 | 2 | 0 | 0 | 0 | 4 | 0 | – | 0 | 2 | 3 | 0 | 1 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 5 | 2 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 6 | 4 | – | 0 | 1 | 0 | 0 | 0 | 0 |
Cytoplasm | 104 | 283 | 91 | 124 | 160 | 161 | 215 | 218 | 43 | 149 | 78 | 144 | 37 | 96 | 88 | 238 | 295 | – | 85 | 249 | 67 | 25 | 126 | 123 |
Endoplasmic Reticulum | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 29 | 24 | – | 1 | 0 | 2 | 0 | 0 | 1 |
Endosome | 0 | 0 | 0 | 1 | 0 | 2 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 9 | 8 | – | 3 | 4 | 0 | 0 | 2 | 2 |
Golgi | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3 | 0 | – | 1 | 0 | 0 | 0 | 0 | 2 |
Mitochondria | 13 | 2 | 0 | 4 | 2 | 9 | 15 | 23 | 36 | 49 | 98 | 75 | 0 | 0 | 1 | 12 | 4 | – | 19 | 20 | 20 | 1 | 6 | 5 |
Nucleus | 0 | 0 | 3 | 0 | 1 | 1 | 1 | 1 | 3 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | – | 0 | 0 | 0 | 0 | 1 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 2 | 3 | 4 | 3 | 6 | 2 | 7 | 5 | 12 | 0 | 0 | 0 | 8 | 3 | – | 1 | 5 | 1 | 1 | 3 | 2 |
Vac/Vac Membrane | 0 | 1 | 0 | 2 | 5 | 1 | 0 | 4 | 0 | 1 | 0 | 1 | 0 | 1 | 0 | 3 | 9 | – | 0 | 3 | 2 | 0 | 5 | 1 |
Unique Cell Count | 112 | 286 | 97 | 130 | 168 | 171 | 231 | 242 | 71 | 185 | 145 | 196 | 37 | 96 | 90 | 260 | 312 | 117 | 300 | 110 | 30 | 156 | 148 | |
Labelled Cell Count | 117 | 291 | 107 | 137 | 171 | 181 | 243 | 267 | 90 | 214 | 185 | 239 | 37 | 97 | 90 | 314 | 349 | 117 | 300 | 110 | 30 | 156 | 148 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 3.2 | 3.8 | 4.4 | 4.6 | 5.2 | 4.4 | 3.9 | 4.6 | 3.2 | 3.6 | 3.0 | 3.3 | 5.0 | 5.2 | 5.3 | 5.3 | 5.4 | 4.7 | – | 4.9 | 5.4 |
Std Deviation (1e-4) | 0.4 | 0.6 | 0.8 | 2.2 | 3.0 | 2.4 | 1.6 | 2.0 | 0.9 | 1.4 | 0.7 | 0.7 | 0.8 | 1.4 | 2.0 | 1.7 | 1.5 | 1.3 | – | 1.5 | 2.0 |
Intensity Change (Log2) | – | – | – | 0.07 | 0.23 | -0.0 | -0.16 | 0.06 | -0.45 | -0.29 | -0.55 | -0.41 | 0.18 | 0.23 | 0.26 | 0.27 | 0.29 | 0.11 | – | 0.14 | 0.28 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Neck | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Site | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cell Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cyto | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endoplasmic Reticulum | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endosome | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Golgi | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Mitochondria | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nuclear Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nuc | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleolus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Peroxisomes | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
SpindlePole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Vac | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cortical Patches | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cytoplasm | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Vacuole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | -0.4357 | -0.5393 | -0.8171 | -1.0072 | 0.3803 | -0.5282 | 1.1692 | 1.1022 | 0.4095 | -0.0639 | 0.1362 | 0.2045 | -0.4311 | 0.6775 | -0.203 | -0.4522 | -0.9037 | 0.2625 |
Actin | 0.0682 | 0.0345 | 0.0518 | 0.0002 | 0.0009 | 0.0011 | 0.1005 | 0.0398 | 0.0317 | 0.0001 | 0.0012 | 0.0031 | 0.0079 | 0.0375 | 0.008 | 0 | 0 | 0.0032 |
Bud | 0.0038 | 0.0008 | 0.0006 | 0.0001 | 0.0001 | 0.0004 | 0.0009 | 0.0006 | 0.0013 | 0.0001 | 0.0001 | 0.0006 | 0.0002 | 0.0253 | 0.0043 | 0.0001 | 0 | 0.0002 |
Bud Neck | 0.0077 | 0.003 | 0.0101 | 0.0002 | 0.0001 | 0.0014 | 0.003 | 0.0006 | 0.0008 | 0.0001 | 0.0002 | 0.001 | 0.0002 | 0.0004 | 0.0003 | 0.0002 | 0.0001 | 0.0005 |
Bud Periphery | 0.0057 | 0.0005 | 0.0009 | 0.0001 | 0.0002 | 0.0004 | 0.0012 | 0.0007 | 0.002 | 0.0002 | 0.0001 | 0.002 | 0.0002 | 0.0038 | 0.0115 | 0.0001 | 0 | 0.0003 |
Bud Site | 0.0157 | 0.0074 | 0.0062 | 0.0002 | 0.0002 | 0.0013 | 0.0063 | 0.003 | 0.0104 | 0.0002 | 0.0002 | 0.0005 | 0.0009 | 0.0175 | 0.007 | 0.0001 | 0 | 0.0003 |
Cell Periphery | 0.0013 | 0.0005 | 0.0003 | 0 | 0.0001 | 0.0006 | 0.0007 | 0.0005 | 0.0004 | 0.0001 | 0.0001 | 0.0003 | 0.0001 | 0.0007 | 0.0004 | 0.0001 | 0 | 0.0001 |
Cytoplasm | 0.1185 | 0.2165 | 0.1602 | 0.0853 | 0.0388 | 0.2764 | 0.1612 | 0.22 | 0.1527 | 0.1144 | 0.0678 | 0.1622 | 0.2017 | 0.2829 | 0.2499 | 0.2009 | 0.1497 | 0.357 |
Cytoplasmic Foci | 0.1234 | 0.2062 | 0.1526 | 0.1422 | 0.0314 | 0.1881 | 0.0825 | 0.0936 | 0.1703 | 0.0695 | 0.2074 | 0.1781 | 0.209 | 0.0921 | 0.1561 | 0.2258 | 0.1546 | 0.2718 |
Eisosomes | 0.0012 | 0.0002 | 0.0008 | 0 | 0.0002 | 0.0001 | 0.001 | 0.0003 | 0.0004 | 0.0001 | 0.0002 | 0.0002 | 0.0001 | 0.0002 | 0.0001 | 0 | 0 | 0.0001 |
Endoplasmic Reticulum | 0.0075 | 0.0049 | 0.0051 | 0.0029 | 0.002 | 0.0107 | 0.0174 | 0.0097 | 0.0048 | 0.0019 | 0.0065 | 0.0047 | 0.0026 | 0.007 | 0.0086 | 0.0008 | 0.0024 | 0.0025 |
Endosome | 0.198 | 0.1632 | 0.1473 | 0.3755 | 0.0151 | 0.1415 | 0.0792 | 0.1055 | 0.2092 | 0.1779 | 0.1503 | 0.1447 | 0.2634 | 0.1338 | 0.2402 | 0.2246 | 0.249 | 0.1425 |
Golgi | 0.0099 | 0.0094 | 0.0086 | 0.0061 | 0.0084 | 0.0279 | 0.0104 | 0.0224 | 0.0109 | 0.0066 | 0.0037 | 0.0115 | 0.0102 | 0.006 | 0.0147 | 0.0031 | 0.0058 | 0.0409 |
Lipid Particles | 0.0484 | 0.0145 | 0.0311 | 0.0587 | 0.0719 | 0.0617 | 0.0329 | 0.0152 | 0.0414 | 0.0013 | 0.0167 | 0.0238 | 0.0662 | 0.013 | 0.0121 | 0.013 | 0.003 | 0.0462 |
Mitochondria | 0.0514 | 0.0033 | 0.0113 | 0.0038 | 0.3071 | 0.0072 | 0.0114 | 0.0148 | 0.0082 | 0.0021 | 0.0021 | 0.0596 | 0.0071 | 0.0224 | 0.019 | 0.0004 | 0.0008 | 0.0266 |
None | 0.2137 | 0.3129 | 0.3779 | 0.256 | 0.3339 | 0.25 | 0.2446 | 0.4227 | 0.2509 | 0.6113 | 0.3553 | 0.3729 | 0.12 | 0.3241 | 0.2354 | 0.3046 | 0.4123 | 0.0804 |
Nuclear Periphery | 0.0039 | 0.0027 | 0.0032 | 0.0029 | 0.0375 | 0.0014 | 0.0674 | 0.0111 | 0.004 | 0.0011 | 0.1183 | 0.0019 | 0.0014 | 0.0028 | 0.0038 | 0.0006 | 0.002 | 0.0014 |
Nucleolus | 0.027 | 0.0005 | 0.0007 | 0.0003 | 0.0007 | 0.0006 | 0.0053 | 0.0005 | 0.0019 | 0.0004 | 0.0033 | 0.0008 | 0.0003 | 0.0004 | 0.0003 | 0.0004 | 0.0003 | 0.0002 |
Nucleus | 0.0077 | 0.002 | 0.0013 | 0.0013 | 0.1337 | 0.001 | 0.0625 | 0.0029 | 0.0274 | 0.0011 | 0.0391 | 0.0015 | 0.0006 | 0.0019 | 0.0012 | 0.0025 | 0.0012 | 0.0011 |
Peroxisomes | 0.008 | 0.0029 | 0.009 | 0.02 | 0.0087 | 0.0079 | 0.0121 | 0.0024 | 0.0287 | 0.0006 | 0.0024 | 0.0092 | 0.072 | 0.0016 | 0.0027 | 0.0009 | 0.0005 | 0.0068 |
Punctate Nuclear | 0.0035 | 0.0039 | 0.0026 | 0.0018 | 0.0018 | 0.0015 | 0.0428 | 0.0019 | 0.0069 | 0.0006 | 0.0168 | 0.0031 | 0.0097 | 0.0031 | 0.0037 | 0.0012 | 0.001 | 0.0023 |
Vacuole | 0.0645 | 0.0089 | 0.0154 | 0.0367 | 0.0034 | 0.0168 | 0.049 | 0.0216 | 0.0331 | 0.0086 | 0.0048 | 0.0112 | 0.024 | 0.0208 | 0.0167 | 0.0197 | 0.0151 | 0.0135 |
Vacuole Periphery | 0.0111 | 0.0014 | 0.0028 | 0.0058 | 0.0039 | 0.002 | 0.0076 | 0.0101 | 0.0026 | 0.0017 | 0.0036 | 0.0072 | 0.0022 | 0.0029 | 0.0039 | 0.001 | 0.0021 | 0.0023 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 6.8428 | 8.6658 | 8.7 | 9.9131 | 7.0237 | 11.9683 | 11.2102 | 9.9143 | 10.3503 | 10.9746 |
Translational Efficiency | 0.8101 | 0.7253 | 0.6581 | 0.4861 | 0.7048 | 0.5652 | 0.6615 | 0.6404 | 0.5774 | 0.5503 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
60 | 1210 | 124 | 345 | 241 | 1303 | 898 | 39 | 301 | 2513 | 1022 | 384 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 626.78 | 674.01 | 960.35 | 885.27 | 705.10 | 701.91 | 874.50 | 847.76 | 689.49 | 688.48 | 884.92 | 881.46 |
Standard Deviation | 63.59 | 84.28 | 115.86 | 103.53 | 67.52 | 96.35 | 96.72 | 118.19 | 73.72 | 91.80 | 103.12 | 105.72 |
Intensity Change Log 2 | — | 0.104811 | 0.615601 | 0.498158 | — | -0.006542 | 0.310631 | 0.265828 | — | 0.046932 | 0.462198 | 0.379833 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.000207 | 0.000524 | 0.002003 | 0.001277 | 0.000124 | 0.000340 | 0.000474 | 0.000201 | 0.000141 | 0.000428 | 0.000659 | 0.001168 |
Bud Neck | 0.033963 | 0.043514 | 0.029257 | 0.022493 | 0.056048 | 0.033142 | 0.033827 | 0.015329 | 0.051645 | 0.038136 | 0.033273 | 0.021766 |
Bud Site | 0.007377 | 0.021260 | 0.023854 | 0.080385 | 0.007105 | 0.020931 | 0.016362 | 0.013426 | 0.007159 | 0.021089 | 0.017271 | 0.073584 |
Cell Periphery | 0.000191 | 0.000123 | 0.000170 | 0.000142 | 0.000109 | 0.000160 | 0.000067 | 0.000135 | 0.000125 | 0.000142 | 0.000080 | 0.000142 |
Cytoplasm | 0.131232* | 0.139317* | 0.035453 | 0.233315* | 0.155766* | 0.115245* | 0.151455* | 0.249169* | 0.150875* | 0.126835* | 0.137380* | 0.234925* |
Cytoplasmic Foci | 0.315331* | 0.328223* | 0.087590 | 0.086294 | 0.325721* | 0.265308* | 0.126519 | 0.071736 | 0.323650* | 0.295601* | 0.121796 | 0.084816 |
Eisosomes | 0.000285 | 0.000144 | 0.000171 | 0.000071 | 0.000185 | 0.000137 | 0.000073 | 0.000091 | 0.000205 | 0.000140 | 0.000085 | 0.000073 |
Endoplasmic Reticulum | 0.012228 | 0.001387 | 0.010903 | 0.002862 | 0.000515 | 0.001151 | 0.002613 | 0.001432 | 0.002850 | 0.001265 | 0.003619 | 0.002717 |
Endosome | 0.220617* | 0.189689 | 0.224532* | 0.060867 | 0.227519* | 0.247508* | 0.198727 | 0.147931 | 0.226143* | 0.219668* | 0.201858* | 0.069709 |
Golgi | 0.038714 | 0.027098 | 0.006346 | 0.007795 | 0.011021 | 0.035299 | 0.001944 | 0.000595 | 0.016541 | 0.031350 | 0.002479 | 0.007064 |
Lipid Particles | 0.052325 | 0.014931 | 0.013782 | 0.004536 | 0.016011 | 0.014722 | 0.005832 | 0.006437 | 0.023250 | 0.014823 | 0.006796 | 0.004729 |
Mitochondria | 0.051247 | 0.037187 | 0.001559 | 0.004143 | 0.010299 | 0.048262 | 0.001366 | 0.057786 | 0.018461 | 0.042929 | 0.001389 | 0.009591 |
Mitotic Spindle | 0.000291 | 0.007550 | 0.058104 | 0.067457 | 0.001017 | 0.013224 | 0.053430 | 0.019134 | 0.000873 | 0.010492 | 0.053997 | 0.062549 |
None | 0.000388 | 0.001504 | 0.002530 | 0.001774 | 0.000521 | 0.001573 | 0.001362 | 0.000861 | 0.000495 | 0.001540 | 0.001504 | 0.001682 |
Nuclear Periphery | 0.000444 | 0.000375 | 0.003596 | 0.001315 | 0.000510 | 0.000399 | 0.002624 | 0.000720 | 0.000497 | 0.000388 | 0.002742 | 0.001254 |
Nuclear Periphery Foci | 0.000251 | 0.000635 | 0.021017 | 0.004563 | 0.000509 | 0.000403 | 0.007327 | 0.003164 | 0.000458 | 0.000515 | 0.008988 | 0.004421 |
Nucleolus | 0.001451 | 0.003106 | 0.040603 | 0.002248 | 0.003482 | 0.002181 | 0.012672 | 0.002301 | 0.003077 | 0.002626 | 0.016061 | 0.002254 |
Nucleus | 0.020964 | 0.019284 | 0.145706* | 0.050058 | 0.053252 | 0.013174 | 0.084160 | 0.062388 | 0.046816 | 0.016116 | 0.091627 | 0.051310 |
Peroxisomes | 0.034277 | 0.033920 | 0.069733 | 0.018108 | 0.026593 | 0.023336 | 0.035893 | 0.007361 | 0.028125 | 0.028432 | 0.039999 | 0.017016 |
Vacuole | 0.067249 | 0.118835 | 0.219215* | 0.348846* | 0.099987 | 0.144899 | 0.261844* | 0.336791* | 0.093461 | 0.132349 | 0.256672* | 0.347621* |
Vacuole Periphery | 0.010968 | 0.011395 | 0.003873 | 0.001452 | 0.003704 | 0.018607 | 0.001429 | 0.003012 | 0.005152 | 0.015134 | 0.001726 | 0.001610 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -2.29 | -5.20 | -3.65 | -2.38 | 3.25 | -4.97 | -4.62 | -2.73 | 1.46 | 2.97 | -5.96 | -6.37 | -4.82 | -2.92 | -0.62 |
Bud Neck | -0.92 | -0.23 | 1.34 | 6.47 | 3.31 | 2.80 | 2.37 | 5.46 | 5.81 | 6.43 | 1.94 | 2.16 | 4.75 | 6.96 | 5.89 |
Bud Site | -5.08 | -5.10 | -5.09 | -3.08 | -2.33 | -6.06 | -5.13 | -1.54 | 0.21 | -0.29 | -8.23 | -6.32 | -5.49 | -3.05 | -3.58 |
Cell Periphery | 1.71 | -0.06 | 2.17 | 1.16 | 3.20 | -2.85 | 3.72 | -0.34 | 1.84 | -2.41 | -1.17 | 3.36 | 1.30 | 2.67 | -1.99 |
Cytoplasm | -0.44 | 3.67 | -3.10 | -5.08 | -11.40 | 3.01 | -0.57 | -1.82 | -2.77 | -1.69 | 2.00 | 0.12 | -3.85 | -6.78 | -4.74 |
Cytoplasmic Foci | -0.54 | 10.26 | 10.84 | 33.53 | 0.68 | 4.33 | 15.90 | 11.08 | 9.79 | 1.83 | 2.40 | 18.36 | 21.56 | 36.97 | 7.74 |
Eisosomes | 1.83 | 1.24 | 2.78 | 6.38 | 7.22 | 3.22 | 7.64 | 3.33 | 1.09 | -1.96 | 3.36 | 5.82 | 6.79 | 8.00 | 3.35 |
Endoplasmic Reticulum | 1.25 | -0.26 | 1.01 | -3.89 | 5.46 | -4.15 | -13.09 | -4.19 | -1.96 | 4.25 | 0.91 | -1.06 | -0.25 | -4.71 | 2.75 |
Endosome | 1.17 | 2.40 | 7.28 | 21.70 | 6.76 | -1.27 | 4.90 | 4.53 | 5.86 | 2.14 | 0.31 | 5.51 | 12.48 | 28.69 | 13.16 |
Golgi | 1.14 | 3.47 | 3.61 | 11.62 | 1.05 | -6.46 | 3.08 | 3.37 | 15.63 | 4.41 | -4.28 | 4.56 | 4.16 | 15.76 | -0.29 |
Lipid Particles | 3.46 | 3.66 | 4.51 | 8.96 | 4.28 | 0.61 | 4.77 | 0.92 | 0.70 | -0.84 | 2.89 | 5.80 | 6.46 | 9.67 | 2.94 |
Mitochondria | 0.92 | 3.29 | 3.31 | 12.79 | 0.40 | -10.26 | 4.22 | -0.54 | 1.19 | -0.95 | -6.05 | 4.78 | 3.47 | 12.60 | -0.92 |
Mitotic Spindle | -6.45 | -5.15 | -6.16 | -5.23 | 2.19 | -5.93 | -10.32 | -1.41 | -0.91 | 0.68 | -7.94 | -11.52 | -6.22 | -4.92 | 0.98 |
None | -2.99 | -2.01 | -4.22 | -0.75 | 0.67 | -1.59 | -6.32 | -1.67 | 0.48 | 1.21 | -2.70 | -6.27 | -4.15 | -0.58 | 0.01 |
Nuclear Periphery | 0.41 | -6.59 | -8.58 | -10.74 | 4.04 | 0.83 | -13.32 | -3.02 | -3.85 | 8.42 | 0.99 | -15.02 | -9.18 | -11.20 | 6.81 |
Nuclear Periphery Foci | -3.15 | -6.45 | -5.38 | -4.91 | 5.18 | 1.02 | -9.11 | -2.56 | -2.60 | 1.30 | -0.78 | -10.96 | -5.69 | -5.61 | 5.20 |
Nucleolus | -2.06 | -5.21 | -1.34 | 0.93 | 5.07 | 1.38 | -7.40 | 0.32 | -0.44 | 6.51 | 0.42 | -9.07 | 0.67 | 0.37 | 9.53 |
Nucleus | 0.20 | -7.08 | -5.96 | -7.76 | 4.27 | 5.63 | -4.75 | -1.39 | -3.47 | 0.56 | 5.19 | -7.38 | -3.68 | -8.87 | 2.64 |
Peroxisomes | 0.01 | -0.98 | 3.74 | 10.42 | 4.18 | 0.95 | 0.29 | 5.31 | 7.27 | 6.62 | -0.03 | 0.08 | 6.07 | 11.36 | 7.63 |
Vacuole | -2.87 | -6.75 | -17.43 | -20.16 | -8.28 | -4.58 | -17.25 | -5.68 | -4.89 | -1.80 | -4.68 | -18.83 | -20.97 | -20.23 | -9.09 |
Vacuole Periphery | -0.11 | 2.16 | 2.78 | 11.69 | 5.60 | -13.87 | 5.06 | 2.25 | 13.06 | -0.21 | -9.29 | 4.43 | 5.07 | 20.97 | 3.15 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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