Standard name | |
---|---|
Human Ortholog | |
Description | RNA helicase in the DEAH-box family; functions in both RNA polymerase I and polymerase II transcript metabolism; catalyzes removal of U2, U5, and U6 snRNPs from the postsplicing lariat-intron ribonucleoprotein complex; required for efficient biogenesis of both small- and large-subunit rRNAs; acts with Sqs1p to promote 20S to 18S rRNA processing catalyzed by endonuclease Nob1p |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 1 | 1 | 8 | 4 | 4 | 6 | 3 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 3 | 1 | 0 | 1 | 4 |
Bud Neck | 0 | 1 | 12 | 3 | 4 | 6 | 9 | 6 | 5 | 10 | 5 | 2 | 4 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 3 | 2 | 5 | 9 |
Bud Site | 0 | 0 | 1 | 0 | 1 | 0 | 17 | 6 | 25 | 15 | 8 | 18 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 2 | 2 | 4 | 8 | 6 | 7 | 10 | 10 | 2 | 3 | 3 | 1 | 10 | 20 | 32 | 13 | 11 | 5 | 0 | 0 | 0 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 3 | 1 | 2 | 1 | 0 | 0 | 0 | 0 | 0 |
Endosome | 1 | 2 | 5 | 12 | 28 | 46 | 35 | 32 | 22 | 6 | 18 | 33 | 2 | 6 | 12 | 1 | 1 | 4 | 1 | 5 | 3 | 1 | 5 | 9 |
Golgi | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 2 | 1 | 1 | 0 | 0 | 0 | 0 | 3 | 0 |
Mitochondria | 23 | 10 | 5 | 0 | 5 | 22 | 41 | 15 | 82 | 62 | 50 | 30 | 38 | 4 | 9 | 93 | 26 | 35 | 0 | 2 | 1 | 0 | 3 | 2 |
Nucleus | 18 | 38 | 52 | 12 | 25 | 12 | 40 | 18 | 31 | 36 | 22 | 22 | 11 | 10 | 13 | 40 | 27 | 18 | 12 | 38 | 32 | 5 | 6 | 6 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 2 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 4 |
Nucleolus | 145 | 245 | 359 | 230 | 377 | 316 | 446 | 302 | 307 | 334 | 284 | 322 | 188 | 192 | 201 | 439 | 375 | 260 | 146 | 219 | 331 | 125 | 169 | 156 |
Peroxisomes | 1 | 0 | 1 | 2 | 2 | 6 | 5 | 3 | 1 | 1 | 2 | 0 | 2 | 2 | 1 | 2 | 1 | 2 | 0 | 0 | 0 | 0 | 2 | 2 |
SpindlePole | 1 | 11 | 42 | 60 | 126 | 112 | 134 | 125 | 107 | 120 | 102 | 171 | 4 | 15 | 11 | 1 | 4 | 3 | 6 | 12 | 44 | 14 | 36 | 50 |
Vac/Vac Membrane | 1 | 6 | 1 | 0 | 13 | 1 | 5 | 3 | 3 | 3 | 1 | 2 | 0 | 3 | 5 | 3 | 8 | 5 | 1 | 10 | 4 | 2 | 7 | 11 |
Unique Cell Count | 158 | 277 | 406 | 250 | 428 | 344 | 520 | 353 | 371 | 388 | 328 | 377 | 204 | 223 | 251 | 490 | 413 | 290 | 174 | 296 | 425 | 155 | 248 | 268 |
Labelled Cell Count | 192 | 316 | 482 | 329 | 591 | 529 | 750 | 524 | 590 | 598 | 499 | 604 | 259 | 253 | 289 | 598 | 455 | 336 | 174 | 296 | 425 | 155 | 248 | 268 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 20.8 | 20.5 | 17.4 | 14.5 | 13.1 | 10.6 | 9.4 | 9.5 | 7.8 | 7.6 | 7.4 | 8.1 | 18.0 | 18.4 | 16.0 | 36.5 | 38.7 | 35.2 | 20.9 | 19.3 | 19.8 |
Std Deviation (1e-4) | 6.1 | 6.4 | 4.4 | 3.7 | 2.9 | 2.3 | 2.1 | 2.2 | 2.0 | 2.0 | 1.9 | 1.9 | 5.7 | 5.7 | 5.2 | 13.9 | 16.3 | 15.6 | 4.7 | 5.2 | 5.3 |
Intensity Change (Log2) | – | – | – | -0.26 | -0.42 | -0.72 | -0.89 | -0.87 | -1.15 | -1.2 | -1.23 | -1.1 | 0.05 | 0.08 | -0.12 | 1.07 | 1.15 | 1.01 | 0.27 | 0.15 | 0.18 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | -1.5 | -2.1 | -1.1 | -1.2 | -1.1 | -1.5 | -0.3 | -1.3 | -2.6 | -0.7 | 0 | -1.9 | -3.8 | -3.5 | -3.0 |
Bud Site | 0 | 0 | 0 | 3.3 | 0 | 5.0 | 3.6 | 0 | 4.1 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 0 | 0 | 1.2 | 1.2 | 1.9 | 0 | 0 | 0 | 0 | 0 | 5.0 | 6.4 | 1.8 | 1.8 | 0 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 2.8 | 3.9 | 6.6 | 4.1 | 5.0 | 3.6 | 0.4 | 3.3 | 4.9 | 0 | 0 | 2.8 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 3.8 | 4.6 | 2.6 | 9.2 | 7.5 | 7.2 | 4.6 | 7.9 | 0 | 2.0 | 8.5 | 3.8 | 6.1 |
Nucleus | -3.4 | -3.5 | -4.6 | -2.6 | -3.7 | -2.0 | -1.6 | -2.7 | -3.3 | -2.8 | -3.4 | -3.2 | -2.3 | -3.0 | -2.9 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 1.5 | -0.2 | 1.6 | -1.2 | -1.2 | -2.3 | -1.0 | -0.8 | -1.3 | 1.4 | -0.8 | -2.9 | 0.6 | 1.1 | 0.5 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 4.7 | 6.9 | 7.5 | 5.9 | 8.3 | 6.5 | 7.2 | 7.0 | 11.0 | -3.7 | -1.5 | -2.7 | -7.1 | -5.8 | -4.9 |
Vacuole | 0 | 3.1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 10.1882 | 15.7235 | 12.905 | 14.7151 | 12.2415 | 13.0957 | 17.2055 | 21.1285 | 17.63 | 16.8607 | 15.2181 | 17.3646 | 16.0099 | 18.5141 | 16.6381 | 15.5279 | 15.8251 | 15.7277 |
Actin | 0.0453 | 0 | 0.0076 | 0.0076 | 0 | 0.0107 | 0.0066 | 0 | 0.0062 | 0.0015 | 0.0013 | 0.0008 | 0.0003 | 0 | 0.001 | 0.0001 | 0 | 0.0093 |
Bud | 0.0008 | 0 | 0.0001 | 0.0001 | 0 | 0.0001 | 0.0003 | 0 | 0.0004 | 0 | 0.0001 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0.0022 | 0.0001 | 0.0003 | 0.0024 | 0 | 0.0003 | 0.0004 | 0.0001 | 0.0002 | 0.0001 | 0.0002 | 0.0001 | 0.0001 | 0.0002 | 0.0001 | 0.0001 | 0.0001 | 0.0002 |
Bud Periphery | 0.001 | 0 | 0.0001 | 0.0002 | 0 | 0.0004 | 0.0006 | 0 | 0.0009 | 0.0001 | 0.0002 | 0.0001 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0.0036 | 0.0002 | 0.0012 | 0.0002 | 0 | 0.0003 | 0.0013 | 0 | 0.0016 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0003 | 0.0001 | 0.0001 |
Cell Periphery | 0.0004 | 0 | 0.0002 | 0.0001 | 0.0001 | 0.0002 | 0.0002 | 0 | 0.0003 | 0.0001 | 0.0002 | 0.0001 | 0.0001 | 0.0001 | 0 | 0.0001 | 0.0001 | 0 |
Cytoplasm | 0.0068 | 0 | 0.0011 | 0.0004 | 0.0001 | 0.0002 | 0.0004 | 0 | 0.0013 | 0 | 0 | 0 | 0.0004 | 0 | 0.0005 | 0.0006 | 0 | 0.0001 |
Cytoplasmic Foci | 0.0118 | 0.0015 | 0.0129 | 0.0039 | 0.019 | 0.0114 | 0.009 | 0.0001 | 0.004 | 0.0008 | 0.0042 | 0.004 | 0.0065 | 0.0001 | 0.0005 | 0.0008 | 0.0012 | 0.0006 |
Eisosomes | 0.0007 | 0 | 0.0002 | 0 | 0 | 0.0014 | 0.0002 | 0 | 0.0002 | 0.0001 | 0.0001 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0.0152 | 0 | 0.001 | 0.0004 | 0 | 0.0012 | 0.0016 | 0 | 0.0006 | 0.0002 | 0.0006 | 0.0001 | 0.0001 | 0 | 0.0004 | 0.0001 | 0 | 0.0001 |
Endosome | 0.0463 | 0 | 0.0121 | 0.0142 | 0.0001 | 0.0035 | 0.0137 | 0 | 0.0084 | 0.0035 | 0.006 | 0.0019 | 0.0039 | 0 | 0.0017 | 0.0001 | 0 | 0.0009 |
Golgi | 0.0136 | 0 | 0.004 | 0.0067 | 0.0001 | 0.0031 | 0.0062 | 0 | 0.0028 | 0.0033 | 0.0051 | 0.0027 | 0.0005 | 0 | 0.0002 | 0 | 0.0002 | 0.0007 |
Lipid Particles | 0.0217 | 0.0002 | 0.0048 | 0.003 | 0.0135 | 0.0147 | 0.0187 | 0.0001 | 0.018 | 0.0204 | 0.0451 | 0.0073 | 0.0018 | 0.0001 | 0.0003 | 0.0003 | 0.0183 | 0.0011 |
Mitochondria | 0.016 | 0.0001 | 0.0164 | 0.0012 | 0.0019 | 0.0049 | 0.0118 | 0.0002 | 0.0134 | 0.0211 | 0.0302 | 0.0023 | 0.0007 | 0 | 0.0001 | 0.0002 | 0.0004 | 0.0002 |
None | 0.0102 | 0 | 0.0021 | 0.0002 | 0.0002 | 0.0004 | 0.0011 | 0 | 0.002 | 0.0001 | 0.0001 | 0 | 0.0004 | 0 | 0.0002 | 0.0004 | 0 | 0.0002 |
Nuclear Periphery | 0.0509 | 0 | 0.0025 | 0.0007 | 0 | 0.0005 | 0.0123 | 0 | 0.0007 | 0.0018 | 0.0046 | 0.0017 | 0.0002 | 0 | 0.0025 | 0.0013 | 0.0003 | 0.0011 |
Nucleolus | 0.6245 | 0.8584 | 0.8558 | 0.8695 | 0.9053 | 0.8851 | 0.8363 | 0.9008 | 0.8855 | 0.874 | 0.8405 | 0.9147 | 0.9099 | 0.9068 | 0.9107 | 0.8767 | 0.9257 | 0.9008 |
Nucleus | 0.0968 | 0.1321 | 0.0667 | 0.0847 | 0.0571 | 0.0527 | 0.0497 | 0.098 | 0.0448 | 0.0693 | 0.0432 | 0.0597 | 0.069 | 0.0894 | 0.0781 | 0.1006 | 0.0506 | 0.0666 |
Peroxisomes | 0.0053 | 0 | 0.0037 | 0.0025 | 0.0004 | 0.0032 | 0.003 | 0 | 0.0014 | 0.0013 | 0.0005 | 0.0002 | 0.0005 | 0 | 0.0001 | 0 | 0.0001 | 0.0002 |
Punctate Nuclear | 0.0121 | 0.0073 | 0.005 | 0.0014 | 0.001 | 0.0053 | 0.0217 | 0.0004 | 0.0016 | 0.0007 | 0.0126 | 0.0034 | 0.0052 | 0.0031 | 0.0032 | 0.0182 | 0.0026 | 0.0178 |
Vacuole | 0.0064 | 0.0001 | 0.0009 | 0.0004 | 0.0003 | 0.0002 | 0.002 | 0.0001 | 0.0037 | 0.0004 | 0.0011 | 0.0002 | 0.0002 | 0.0002 | 0.0001 | 0.0001 | 0.0001 | 0.0001 |
Vacuole Periphery | 0.0083 | 0 | 0.0013 | 0.0003 | 0.0007 | 0.0002 | 0.0028 | 0 | 0.0019 | 0.0011 | 0.0039 | 0.0006 | 0.0002 | 0.0001 | 0.0001 | 0.0001 | 0.0002 | 0.0001 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 100.5707 | 100.6069 | 68.6188 | 62.6454 | 70.7855 | 56.4799 | 59.3027 | 45.2841 | 40.4895 | 71.8004 |
Translational Efficiency | 1.231 | 1.2071 | 1.248 | 1.0933 | 1.2881 | 1.3035 | 1.1946 | 1.181 | 1.0775 | 1.0704 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✔ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
1298 | 760 | 136 | 1266 | 1627 | 1926 | 178 | 2588 | 2925 | 2686 | 314 | 3854 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 1303.51 | 1751.73 | 2293.94 | 2767.25 | 1443.19 | 1707.18 | 2473.70 | 2887.30 | 1381.21 | 1719.79 | 2395.84 | 2847.86 |
Standard Deviation | 268.23 | 400.07 | 462.96 | 726.86 | 328.10 | 377.42 | 448.44 | 751.46 | 310.84 | 384.49 | 463.43 | 745.60 |
Intensity Change Log 2 | — | 0.426379 | 0.815426 | 1.086051 | — | 0.242354 | 0.777409 | 1.000460 | — | 0.332618 | 0.795576 | 1.041713 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.000160 | 0.000253 | 0.000322 | 0.000473 | 0.000209 | 0.000256 | 0.000256 | 0.000416 | 0.000187 | 0.000255 | 0.000285 | 0.000435 |
Bud Neck | 0.001148 | 0.001428 | 0.003770 | 0.005528 | 0.001050 | 0.001620 | 0.002968 | 0.003067 | 0.001093 | 0.001566 | 0.003315 | 0.003876 |
Bud Site | 0.000498 | 0.000394 | 0.001272 | 0.001537 | 0.000333 | 0.000373 | 0.000984 | 0.000655 | 0.000406 | 0.000379 | 0.001109 | 0.000945 |
Cell Periphery | 0.000029 | 0.000016 | 0.000023 | 0.000076 | 0.000021 | 0.000026 | 0.000020 | 0.000055 | 0.000024 | 0.000023 | 0.000021 | 0.000062 |
Cytoplasm | 0.000149 | 0.000482 | 0.000047 | 0.000450 | 0.000229 | 0.000165 | 0.000062 | 0.000239 | 0.000194 | 0.000254 | 0.000055 | 0.000308 |
Cytoplasmic Foci | 0.005311 | 0.003847 | 0.010746 | 0.009037 | 0.004839 | 0.002562 | 0.008566 | 0.002935 | 0.005048 | 0.002926 | 0.009510 | 0.004939 |
Eisosomes | 0.000253 | 0.000221 | 0.000196 | 0.000195 | 0.000339 | 0.000350 | 0.000157 | 0.000378 | 0.000301 | 0.000313 | 0.000174 | 0.000318 |
Endoplasmic Reticulum | 0.000240 | 0.000277 | 0.000124 | 0.000122 | 0.000220 | 0.000167 | 0.000070 | 0.000109 | 0.000229 | 0.000198 | 0.000093 | 0.000113 |
Endosome | 0.000437 | 0.000118 | 0.000036 | 0.000391 | 0.000056 | 0.000119 | 0.000025 | 0.000100 | 0.000225 | 0.000119 | 0.000030 | 0.000196 |
Golgi | 0.000305 | 0.000256 | 0.000625 | 0.001381 | 0.000265 | 0.000299 | 0.000345 | 0.000257 | 0.000283 | 0.000287 | 0.000466 | 0.000626 |
Lipid Particles | 0.000090 | 0.000265 | 0.000033 | 0.000065 | 0.000048 | 0.000092 | 0.000014 | 0.000016 | 0.000067 | 0.000141 | 0.000022 | 0.000032 |
Mitochondria | 0.001019 | 0.000731 | 0.001289 | 0.003777 | 0.000825 | 0.001254 | 0.001057 | 0.001936 | 0.000911 | 0.001106 | 0.001158 | 0.002541 |
Mitotic Spindle | 0.004091 | 0.017076 | 0.016646 | 0.022305 | 0.003892 | 0.006109 | 0.016578 | 0.004258 | 0.003980 | 0.009212 | 0.016607 | 0.010186 |
None | 0.000596 | 0.000325 | 0.000314 | 0.000636 | 0.000696 | 0.000430 | 0.000297 | 0.000506 | 0.000652 | 0.000400 | 0.000304 | 0.000549 |
Nuclear Periphery | 0.000063 | 0.000113 | 0.000131 | 0.000838 | 0.000068 | 0.000297 | 0.000100 | 0.000221 | 0.000066 | 0.000245 | 0.000113 | 0.000424 |
Nuclear Periphery Foci | 0.001557 | 0.000541 | 0.000157 | 0.000283 | 0.000685 | 0.000313 | 0.000054 | 0.000118 | 0.001072 | 0.000377 | 0.000099 | 0.000172 |
Nucleolus | 0.700641* | 0.670865* | 0.659401* | 0.719514* | 0.698767* | 0.693797* | 0.705949* | 0.700009* | 0.699599* | 0.687308* | 0.685788* | 0.706417* |
Nucleus | 0.167861* | 0.180055* | 0.110184 | 0.159533* | 0.182121* | 0.212096* | 0.135542* | 0.235626* | 0.175793* | 0.203030* | 0.124559 | 0.210630* |
Peroxisomes | 0.114546* | 0.121335* | 0.193396* | 0.069754 | 0.104403* | 0.078456 | 0.125997* | 0.046045 | 0.108904* | 0.090589 | 0.155189* | 0.053833 |
Vacuole | 0.000651 | 0.001013 | 0.000664 | 0.001484 | 0.000550 | 0.000641 | 0.000390 | 0.001051 | 0.000595 | 0.000747 | 0.000509 | 0.001193 |
Vacuole Periphery | 0.000356 | 0.000390 | 0.000623 | 0.002621 | 0.000384 | 0.000578 | 0.000570 | 0.002002 | 0.000372 | 0.000525 | 0.000593 | 0.002205 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -3.06 | -6.67 | -15.16 | -6.05 | -5.06 | -2.86 | -1.94 | -12.65 | -9.53 | -5.43 | -4.86 | -5.46 | -19.83 | -11.33 | -7.11 |
Bud Neck | -1.50 | -6.89 | -10.47 | -9.26 | -3.78 | -3.06 | -5.21 | -8.90 | -5.58 | -0.37 | -3.22 | -8.37 | -13.48 | -10.20 | -2.21 |
Bud Site | 0.46 | -4.75 | -6.23 | -7.66 | -1.45 | -0.09 | -3.75 | -3.40 | -3.36 | 1.71 | 0.37 | -5.92 | -6.39 | -7.49 | 1.01 |
Cell Periphery | 1.43 | 0.44 | -4.65 | -6.47 | -5.71 | -0.86 | -0.06 | -4.19 | -2.92 | -3.47 | -0.00 | 0.24 | -5.77 | -5.30 | -5.78 |
Cytoplasm | -0.90 | 2.82 | -3.31 | 0.17 | -4.17 | 0.59 | 2.32 | -0.13 | -0.76 | -3.47 | -0.57 | 3.02 | -2.19 | -0.28 | -5.50 |
Cytoplasmic Foci | 2.02 | -3.51 | -3.82 | -4.95 | 1.17 | 3.16 | -1.47 | 2.44 | -1.06 | 2.42 | 4.12 | -3.15 | -0.17 | -4.48 | 3.09 |
Eisosomes | 1.30 | 2.94 | 3.53 | 1.12 | 0.48 | -0.33 | 8.58 | -0.82 | -0.52 | -5.32 | -0.52 | 8.75 | -0.46 | -0.03 | -4.92 |
Endoplasmic Reticulum | -0.51 | 3.20 | 3.02 | 1.73 | -0.34 | 3.15 | 10.71 | 7.59 | 4.17 | -4.63 | 0.85 | 7.15 | 6.11 | 2.89 | -2.27 |
Endosome | 0.80 | 1.08 | 0.14 | -1.11 | -1.68 | -1.00 | 1.90 | -0.69 | 0.27 | -1.29 | 0.58 | 1.20 | 0.19 | -0.69 | -2.09 |
Golgi | 0.71 | -4.55 | -1.35 | -1.42 | -0.82 | -0.20 | -2.00 | 0.17 | 0.27 | 1.34 | 0.05 | -4.73 | -1.25 | -1.19 | -0.37 |
Lipid Particles | -0.94 | 1.45 | 0.78 | 1.21 | -1.25 | -0.90 | 2.56 | 2.48 | 1.94 | -0.52 | -1.10 | 2.33 | 1.90 | 1.99 | -1.02 |
Mitochondria | 0.59 | -0.58 | -3.03 | -3.89 | -3.16 | -1.88 | -1.72 | -7.72 | -2.73 | -4.51 | -0.76 | -1.17 | -4.80 | -4.60 | -4.83 |
Mitotic Spindle | -3.48 | -3.18 | -6.94 | -1.65 | -1.01 | -1.60 | -2.08 | -0.82 | 0.97 | 1.95 | -3.63 | -3.36 | -6.08 | -1.30 | 1.61 |
None | 3.33 | 3.53 | -0.92 | -5.79 | -6.17 | 2.02 | 3.23 | 1.46 | -1.23 | -4.41 | 2.96 | 4.52 | 0.98 | -3.26 | -7.27 |
Nuclear Periphery | -1.95 | -3.65 | -3.24 | -3.00 | -2.92 | -1.40 | -1.77 | -6.52 | 0.39 | -4.09 | -1.53 | -3.61 | -4.54 | -1.31 | -3.83 |
Nuclear Periphery Foci | 1.96 | 3.03 | 2.78 | 1.74 | -2.06 | 2.05 | 3.58 | 3.20 | 3.83 | -5.12 | 2.90 | 4.26 | 3.95 | 3.43 | -3.16 |
Nucleolus | 3.36 | 3.37 | -0.96 | -3.89 | -3.77 | 0.69 | -0.05 | 0.11 | -0.58 | 0.10 | 2.36 | 2.16 | -0.37 | -2.62 | -2.33 |
Nucleus | -1.45 | 4.98 | 0.46 | 1.73 | -4.53 | -4.73 | 4.06 | -8.41 | -3.82 | -8.81 | -5.48 | 6.25 | -7.57 | -1.88 | -10.48 |
Peroxisomes | -1.24 | -6.61 | 10.95 | 10.67 | 10.35 | 7.58 | -3.03 | 19.37 | 13.63 | 10.33 | 6.27 | -6.75 | 22.58 | 16.46 | 14.43 |
Vacuole | -0.66 | -0.74 | -3.36 | -0.47 | -2.90 | -0.66 | 2.01 | -2.32 | -1.74 | -3.23 | -0.81 | 0.53 | -3.65 | -1.48 | -3.98 |
Vacuole Periphery | -1.21 | -6.05 | -4.10 | -4.02 | -3.53 | -3.17 | -3.60 | -5.90 | -5.07 | -5.13 | -3.49 | -6.42 | -7.11 | -6.41 | -6.13 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
---|
Gene | Images |
---|