Standard name
Human Ortholog
Description Protein involved in the initiation of DNA replication; required for proper assembly of replication proteins at the origins of replication; interacts with Cdc45p; localizes to nuclear foci that become diffuse upon DNA replication stress; homologous to the human Treslin/Ticrr protein
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.27 0.4 0.85 0.5 0.7 0.63 0.62 – 0.53 0.5 0.28 0.45 0.52 0.59 0.7 0.24 0.24 0.22 0.26 0.24 0.25 0.32 0.39 0.4
Endoplasmic Reticulum 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0.07 0.09 0.07 0 0 0 0 0 0
Endosome 0 0.08 0.05 0.09 0 0.05 0 – 0 0 0 0 0 0 0 0.19 0.25 0.24 0.09 0.06 0 0 0 0
Golgi 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.76 0 0 0.09 0 0.22 0.22 – 0.42 0.4 0.72 0.49 0 0 0 0.07 0 0 0.08 0.07 0.16 0.05 0.07 0.05
Nucleus 0 0.17 0 0.18 0.05 0.07 0.09 – 0.07 0.07 0.09 0.06 0.29 0.2 0.15 0.19 0.2 0.2 0.1 0.19 0.08 0.11 0.06 0.07
Nuclear Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0.05 0 0 0.05 0 0.08 – 0.06 0.09 0.05 0.11 0.07 0.05 0 0 0.09 0.15 0.12 0.12 0.1 0.24 0.22 0.18
Vac/Vac Membrane 0.12 0.42 0.12 0.18 0.16 0.13 0.06 – 0 0.05 0 0 0.14 0.19 0.19 0.37 0.29 0.33 0.27 0.24 0.27 0.12 0.12 0.16
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 1 0 0 – 0 0 0 0 0 0 0 0 0 0 2 1 0 0 2 2
Bud 0 0 0 0 1 1 2 – 3 1 11 4 0 0 0 0 0 0 0 3 0 1 2 4
Bud Neck 0 0 0 0 4 1 4 – 6 2 2 11 2 1 2 0 0 0 0 1 0 0 3 6
Bud Site 0 0 0 0 0 0 0 – 5 2 12 8 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 – 0 1 0 1 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 11 62 34 11 104 161 189 – 142 136 88 141 68 122 140 32 36 27 26 55 33 21 96 109
Endoplasmic Reticulum 0 0 0 0 1 0 1 – 0 0 1 0 0 0 0 10 13 8 0 0 1 0 1 0
Endosome 0 12 2 2 4 12 7 – 6 3 1 2 5 8 6 26 38 30 9 13 2 2 5 9
Golgi 0 0 1 0 0 1 0 – 0 0 0 0 0 0 1 2 1 1 0 0 0 0 1 0
Mitochondria 31 5 0 2 3 56 67 – 112 110 231 153 3 2 0 9 6 5 8 15 20 3 17 14
Nucleus 0 26 0 4 8 19 28 – 20 18 28 19 38 41 29 25 30 24 10 42 10 7 14 18
Nuclear Periphery 0 0 0 0 0 0 0 – 0 2 0 0 0 0 0 0 0 1 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 1 – 0 0 2 1 0 0 0 1 0 2 0 4 0 0 5 1
Peroxisomes 0 0 0 0 0 0 1 – 0 0 0 1 0 0 0 3 0 1 0 0 0 0 0 0
SpindlePole 1 8 0 0 7 4 23 – 16 25 16 35 9 10 0 5 14 18 12 28 13 16 53 48
Vac/Vac Membrane 5 65 5 4 23 33 19 – 5 13 10 10 18 39 38 50 44 40 27 55 36 8 30 44
Unique Cell Count 41 156 40 22 148 254 303 268 272 319 314 130 208 199 134 152 123 102 228 132 68 245 276
Labelled Cell Count 48 178 42 23 156 288 342 315 313 402 386 143 223 216 163 182 157 102 228 132 68 245 276


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.3 4.7 5.5 5.3 5.6 4.2 4.5 – 3.7 4.0 3.3 3.8 5.2 5.1 5.3 6.4 6.2 6.2 – 5.0 5.2
Std Deviation (1e-4) 0.3 0.6 1.5 1.5 1.8 1.1 1.9 – 1.1 1.4 0.7 1.4 1.1 1.2 1.6 1.8 1.9 2.0 – 1.4 1.2
Intensity Change (Log2) – – – -0.05 0.03 -0.37 -0.27 – -0.56 -0.47 -0.73 -0.54 -0.08 -0.11 -0.05 0.23 0.18 0.19 – -0.13 -0.07


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – 0 0 0
Bud Neck – – – – – – – – – – – – 0 0 0
Bud Site – – – – – – – – – – – – 0 0 0
Cell Periphery – – – – – – – – – – – – 0 0 0
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – 0 0 0
Endosome – – – – – – – – – – – – 0 0 0
Golgi – – – – – – – – – – – – 0 0 0
Mitochondria – – – – – – – – – – – – 0 0 0
Nuclear Periphery – – – – – – – – – – – – 0 0 0
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – 0 0 0
Peroxisomes – – – – – – – – – – – – 0 0 0
SpindlePole – – – – – – – – – – – – 0 0 0
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – 0 0 0
Cytoplasm – – – – – – – – – – – – 0 0 0
Nucleus – – – – – – – – – – – – 0 0 0
Vacuole – – – – – – – – – – – – 3.0 2.1 2.5

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration -1.1469 -1.3413 -1.2872 -1.8047 -1.2975 -1.25 -0.3862 -0.0423 -0.2115 -0.2857 -0.2262 -0.3275 -1.0642 -0.9626 -0.9777 -0.9267 -1.0847 -0.8934
Actin 0.0289 0.0021 0.0149 0.0006 0.0012 0.0139 0.0156 0.0002 0.0011 0.0022 0.0077 0.0012 0.0252 0.0002 0.0008 0.0005 0.0046 0.003
Bud 0.0009 0.0024 0.0003 0.0001 0.0001 0.0005 0.0025 0.0002 0.0019 0.0004 0.0002 0.002 0.0004 0.0001 0.0001 0.0001 0.0001 0.0001
Bud Neck 0.0022 0.0007 0.0006 0.0003 0.0005 0.0045 0.0037 0.0005 0.0012 0.0005 0.0009 0.0033 0.0093 0.0005 0.0008 0.0009 0.0013 0.0055
Bud Periphery 0.0009 0.0037 0.0008 0.0001 0.0001 0.0006 0.0018 0.0002 0.0007 0.0012 0.0004 0.0042 0.0008 0.0001 0.0001 0.0001 0.0002 0.0002
Bud Site 0.0067 0.0036 0.0008 0.0001 0.0001 0.0006 0.0121 0.0023 0.011 0.0005 0.0002 0.0003 0.0044 0.0012 0.0006 0.0002 0.0002 0.0002
Cell Periphery 0.0003 0.0001 0.0001 0.0001 0 0.0001 0.0007 0.0001 0.0003 0 0.0001 0.0001 0.0003 0 0 0 0 0
Cytoplasm 0.1248 0.168 0.1408 0.2468 0.1253 0.1726 0.1373 0.121 0.1165 0.0923 0.1038 0.1367 0.1599 0.2467 0.147 0.1431 0.1944 0.1663
Cytoplasmic Foci 0.0488 0.0097 0.0323 0.0074 0.0176 0.0354 0.0496 0.0174 0.011 0.0292 0.0103 0.0165 0.0237 0.0148 0.0101 0.0074 0.0064 0.0109
Eisosomes 0.0003 0.0002 0.0004 0.0002 0.0001 0.0001 0.0006 0.0001 0.0002 0.0001 0.0003 0.0001 0.0003 0 0.0001 0.0001 0.0001 0
Endoplasmic Reticulum 0.0093 0.0134 0.0096 0.0067 0.0035 0.0053 0.0044 0.0032 0.0033 0.0027 0.0043 0.003 0.0055 0.0031 0.0032 0.0035 0.0031 0.003
Endosome 0.0376 0.0082 0.0245 0.0061 0.0048 0.0154 0.0294 0.0052 0.0189 0.0189 0.0179 0.0052 0.0168 0.0062 0.0052 0.0058 0.0078 0.0069
Golgi 0.0202 0.0004 0.0055 0.0001 0.0003 0.007 0.0126 0.0002 0.0028 0.0039 0.0016 0.0013 0.003 0.0002 0.0003 0.0001 0.0012 0.0005
Lipid Particles 0.022 0.0029 0.0086 0.0006 0.0009 0.0072 0.0254 0.0012 0.011 0.0052 0.0026 0.007 0.0081 0.0005 0.0023 0.0004 0.0045 0.0018
Mitochondria 0.0126 0.0007 0.003 0.0011 0.0006 0.0176 0.0057 0.0014 0.0064 0.0068 0.0144 0.0022 0.0028 0.0007 0.0081 0.0011 0.0011 0.0008
None 0.3712 0.4953 0.3788 0.5813 0.6099 0.3677 0.4272 0.5797 0.5314 0.4436 0.5718 0.458 0.342 0.3823 0.4188 0.4001 0.3431 0.3025
Nuclear Periphery 0.0199 0.0257 0.0429 0.0233 0.043 0.0158 0.0202 0.012 0.0243 0.0378 0.0183 0.0117 0.0179 0.0148 0.0237 0.015 0.0323 0.0176
Nucleolus 0.0062 0.0037 0.003 0.0008 0.0013 0.0052 0.0056 0.0061 0.0055 0.005 0.0031 0.007 0.0072 0.0043 0.0046 0.0042 0.0044 0.0058
Nucleus 0.1629 0.1808 0.2418 0.1072 0.1244 0.2206 0.1432 0.181 0.196 0.2352 0.1808 0.248 0.2413 0.2807 0.302 0.3231 0.2981 0.3617
Peroxisomes 0.0163 0.0004 0.015 0.0002 0.0013 0.0114 0.0157 0.0005 0.006 0.0187 0.0031 0.004 0.0056 0.0003 0.0004 0.0003 0.0025 0.0011
Punctate Nuclear 0.1016 0.0745 0.0727 0.0137 0.0642 0.0956 0.0808 0.0646 0.045 0.0933 0.0558 0.0864 0.1208 0.04 0.0697 0.0926 0.0924 0.1101
Vacuole 0.0048 0.0028 0.0028 0.0026 0.0006 0.002 0.004 0.0024 0.004 0.001 0.0013 0.0014 0.0036 0.0027 0.0015 0.0012 0.0013 0.0015
Vacuole Periphery 0.0016 0.0006 0.0009 0.0007 0.0003 0.0008 0.0018 0.0006 0.0014 0.0015 0.0009 0.0004 0.001 0.0004 0.0005 0.0004 0.0008 0.0004

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 10.1709 6.3891 5.4704 9.3599 12.8306 6.5937 10.6838 9.3909 9.1912 11.487
Translational Efficiency 0.3632 0.3751 0.3674 0.3169 0.169 0.368 0.2277 0.2291 0.2391 0.2128

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
2544 1783 1108 1337 1839 1587 521 84 4383 3370 1629 1421

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 634.42 741.33 860.63 842.10 681.88 707.07 906.71 907.52 654.33 725.20 875.37 845.97
Standard Deviation 63.24 95.22 96.37 118.26 75.69 96.59 84.97 98.62 72.62 97.38 95.33 118.20
Intensity Change Log 2 — 0.224678 0.439955 0.408553 — 0.052335 0.411123 0.412412 — 0.137972 0.425091 0.410553

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000034 0.000264 0.000628 0.000908 0.000098 0.000250 0.000312 0.001128 0.000061 0.000258 0.000527 0.000921
Bud Neck 0.007749 0.014893 0.008445 0.019550 0.006379 0.012741 0.003036 0.005195 0.007174 0.013880 0.006715 0.018701
Bud Site 0.001159 0.004487 0.005052 0.019555 0.001079 0.006617 0.004818 0.007896 0.001125 0.005490 0.004977 0.018865
Cell Periphery 0.000070 0.000106 0.000113 0.000212 0.000112 0.000172 0.000132 0.000256 0.000088 0.000137 0.000119 0.000214
Cytoplasm 0.444363* 0.334156* 0.049632 0.136525* 0.323477* 0.313409* 0.090890 0.128236* 0.393642* 0.324386* 0.062827 0.136035*
Cytoplasmic Foci 0.089852 0.090484 0.001347 0.005790 0.109781 0.157450 0.006827 0.013686 0.098214 0.122020 0.003100 0.006257
Eisosomes 0.000047 0.000058 0.000039 0.000037 0.000071 0.000103 0.000065 0.000082 0.000057 0.000079 0.000048 0.000040
Endoplasmic Reticulum 0.000497 0.001501 0.006284 0.007158 0.001114 0.001617 0.004152 0.012855 0.000756 0.001556 0.005602 0.007495
Endosome 0.001936 0.006101 0.001498 0.007207 0.003776 0.010725 0.002136 0.012984 0.002708 0.008278 0.001702 0.007548
Golgi 0.000488 0.001741 0.000113 0.007934 0.001191 0.003906 0.000122 0.000671 0.000783 0.002761 0.000116 0.007505
Lipid Particles 0.005703 0.009718 0.001152 0.001238 0.014252 0.018934 0.003754 0.005190 0.009290 0.014058 0.001984 0.001471
Mitochondria 0.003342 0.009205 0.001253 0.003953 0.004544 0.014788 0.000494 0.036537 0.003847 0.011834 0.001010 0.005879
Mitotic Spindle 0.001514 0.011221 0.005211 0.042386 0.005837 0.011911 0.008308 0.038640 0.003328 0.011546 0.006201 0.042164
None 0.011429 0.009959 0.005246 0.009428 0.010438 0.009994 0.008163 0.009376 0.011013 0.009976 0.006179 0.009425
Nuclear Periphery 0.001270 0.003269 0.001981 0.004619 0.002425 0.002550 0.001607 0.006760 0.001754 0.002931 0.001861 0.004745
Nuclear Periphery Foci 0.000540 0.001782 0.001181 0.003981 0.001783 0.001522 0.000865 0.008420 0.001062 0.001659 0.001080 0.004244
Nucleolus 0.005723 0.011607 0.005385 0.005500 0.010528 0.012075 0.004306 0.002215 0.007739 0.011827 0.005040 0.005306
Nucleus 0.414084* 0.464440* 0.893115* 0.685483* 0.489625* 0.383491* 0.835941* 0.625545* 0.445779* 0.426319* 0.874829* 0.681939*
Peroxisomes 0.002438 0.005384 0.000294 0.000828 0.004294 0.013756 0.001118 0.000601 0.003217 0.009327 0.000557 0.000815
Vacuole 0.007534 0.018397 0.011786 0.035420 0.008839 0.022127 0.022803 0.080905 0.008082 0.020154 0.015309 0.038109
Vacuole Periphery 0.000228 0.001227 0.000245 0.002291 0.000358 0.001860 0.000152 0.002824 0.000282 0.001525 0.000215 0.002323

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -4.22 -14.89 -14.32 -8.68 0.48 -3.78 -7.46 -6.96 -6.24 -3.79 -5.71 -16.41 -14.95 -10.93 -1.18
Bud Neck -4.93 -5.08 -7.28 -2.75 -2.80 -5.10 -0.54 -2.13 0.70 -1.88 -6.91 -5.15 -8.01 -3.63 -4.39
Bud Site -4.36 -5.21 -8.25 -5.78 -4.29 -4.77 -2.53 -2.35 -1.18 -1.35 -6.42 -5.69 -8.58 -5.46 -4.72
Cell Periphery -4.45 -7.03 -8.59 -4.56 -3.30 -3.74 -2.50 -3.29 -0.66 -2.02 -5.82 -6.55 -7.51 -2.62 -3.39
Cytoplasm 14.28 67.94* 50.19 29.84 -11.38 1.20 29.51 14.03 13.20 -0.58 12.17 70.53 50.26 35.53 -9.47
Cytoplasmic Foci 0.27 31.65 30.93 26.06 -3.77 -7.31 28.90 24.00 28.56 -0.10 -5.56 43.20 42.92 41.53 -0.57
Eisosomes -5.24 -3.51 -5.69 -0.62 -1.00 -4.95 -3.03 -3.88 -1.46 -1.84 -7.28 -3.82 -3.29 3.46 0.90
Endoplasmic Reticulum -10.92 -22.20 -21.53 -19.04 -2.95 -2.29 -12.02 -8.16 -7.90 -4.88 -7.30 -24.91 -22.37 -20.18 -4.78
Endosome -8.06 0.50 -2.73 3.92 -3.03 -7.15 2.94 -1.05 2.82 -2.07 -10.75 3.09 -1.49 6.66 -2.98
Golgi -2.58 2.52 -1.93 -0.65 -2.22 -3.42 2.77 2.54 5.23 -0.69 -4.42 3.52 -1.60 0.36 -2.22
Lipid Particles -5.38 10.16 10.44 12.30 -0.11 -2.56 9.36 9.01 10.46 -0.54 -5.62 14.05 17.06 18.61 1.13
Mitochondria -6.37 4.04 0.64 7.17 -4.45 -6.38 6.66 -0.38 1.53 -1.18 -8.95 7.27 1.28 9.46 -4.57
Mitotic Spindle -6.54 -5.78 -9.46 -5.78 -4.06 -3.01 -0.89 -2.49 -1.79 -2.09 -6.82 -5.22 -9.30 -6.29 -5.18
None 1.32 7.52 3.97 2.65 -3.69 1.31 3.11 2.29 1.54 0.31 1.86 8.01 4.84 3.10 -2.51
Nuclear Periphery -5.58 -9.28 -7.95 -4.63 -5.41 -0.59 -1.08 -2.68 -2.59 -2.56 -5.18 -7.98 -7.77 -5.74 -5.99
Nuclear Periphery Foci -4.74 -5.31 -5.89 -2.49 -0.25 1.21 -1.15 -2.04 -2.21 -1.76 -2.77 -4.79 -5.09 -3.66 -1.00
Nucleolus -5.62 -5.24 -1.15 4.00 3.86 0.97 0.22 10.02 9.16 5.39 -3.69 -3.44 1.98 4.75 4.47
Nucleus -5.13 -53.42 -30.74 -23.28 14.03 9.76 -27.64 -4.85 -7.55 4.48 2.69 -58.57 -29.76 -30.13 14.06
Peroxisomes -5.64 8.69 8.17 11.33 -0.60 -8.03 7.54 12.05 12.94 1.86 -9.91 12.29 13.69 16.45 1.02
Vacuole -9.18 -6.15 -15.59 -12.09 -11.80 -8.04 -7.37 -5.62 -5.07 -4.20 -12.23 -9.41 -16.32 -12.85 -10.74
Vacuole Periphery -4.78 -1.12 -2.42 0.89 -2.26 -4.46 3.74 -0.71 2.40 -1.20 -6.43 1.22 -2.29 1.90 -2.45
Standard name
Human Ortholog
Description Protein involved in the initiation of DNA replication; required for proper assembly of replication proteins at the origins of replication; interacts with Cdc45p; localizes to nuclear foci that become diffuse upon DNA replication stress; homologous to the human Treslin/Ticrr protein
Localization
Cell Percentages nucleus (41%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-3

Sld3

Sld3


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Sld3-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available