Standard name | |
---|---|
Human Ortholog | |
Description | Golgi membrane protein with similarity to mammalian CASP; genetic interactions with GOS1 (encoding a Golgi snare protein) suggest a role in Golgi function |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 9 | 37 | 7 | 15 | 11 | 1 | 0 | – | 0 | 1 | 1 | 1 | 22 | 11 | 2 | 18 | 7 | 6 | 21 | 37 | 13 | 17 | 21 | 29 |
Bud | 4 | 11 | 0 | 1 | 2 | 0 | 1 | – | 2 | 4 | 6 | 4 | 3 | 3 | 0 | 1 | 1 | 0 | 1 | 4 | 0 | 2 | 2 | 6 |
Bud Neck | 0 | 0 | 1 | 0 | 0 | 0 | 0 | – | 1 | 4 | 0 | 1 | 0 | 0 | 1 | 1 | 0 | 0 | 3 | 3 | 1 | 5 | 1 | 6 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 2 | 2 | 1 | 1 | 2 | 0 | 2 | – | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 37 | 41 | 32 | 48 | 109 | 77 | 126 | – | 94 | 119 | 81 | 105 | 26 | 50 | 47 | 37 | 24 | 26 | 13 | 9 | 15 | 22 | 25 | 30 |
Endoplasmic Reticulum | 65 | 59 | 19 | 26 | 18 | 7 | 3 | – | 4 | 2 | 6 | 11 | 60 | 33 | 14 | 9 | 6 | 8 | 32 | 42 | 18 | 16 | 20 | 31 |
Endosome | 31 | 43 | 12 | 34 | 24 | 14 | 13 | – | 7 | 7 | 6 | 4 | 44 | 100 | 64 | 77 | 89 | 95 | 75 | 69 | 26 | 46 | 43 | 65 |
Golgi | 0 | 3 | 0 | 0 | 1 | 0 | 0 | – | 0 | 1 | 0 | 0 | 2 | 1 | 0 | 9 | 11 | 4 | 14 | 35 | 6 | 6 | 11 | 19 |
Mitochondria | 27 | 4 | 0 | 5 | 1 | 3 | 19 | – | 46 | 60 | 149 | 108 | 0 | 1 | 1 | 13 | 10 | 3 | 31 | 19 | 29 | 35 | 40 | 39 |
Nucleus | 0 | 0 | 1 | 2 | 1 | 3 | 1 | – | 0 | 1 | 2 | 7 | 2 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 2 |
Nuclear Periphery | 1 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 1 | 2 | 1 |
Nucleolus | 0 | 0 | 0 | 1 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 2 | 1 | 1 | 0 | 1 | 1 | 1 | – | 0 | 0 | 0 | 0 | 1 | 5 | 1 | 0 | 0 | 1 | 7 | 2 | 0 | 5 | 5 | 5 |
SpindlePole | 14 | 5 | 1 | 11 | 8 | 1 | 2 | – | 3 | 8 | 3 | 8 | 26 | 27 | 10 | 29 | 23 | 25 | 6 | 2 | 2 | 10 | 6 | 6 |
Vac/Vac Membrane | 0 | 15 | 5 | 3 | 6 | 4 | 5 | – | 5 | 6 | 6 | 6 | 1 | 7 | 5 | 19 | 19 | 23 | 10 | 7 | 11 | 11 | 11 | 13 |
Unique Cell Count | 158 | 174 | 70 | 119 | 165 | 103 | 158 | 153 | 191 | 223 | 226 | 150 | 191 | 131 | 174 | 156 | 150 | 217 | 239 | 131 | 188 | 198 | 263 | |
Labelled Cell Count | 192 | 221 | 80 | 147 | 184 | 111 | 173 | 163 | 213 | 261 | 257 | 187 | 239 | 147 | 217 | 190 | 192 | 217 | 239 | 131 | 188 | 198 | 263 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 5.3 | 8.2 | 7.5 | 7.6 | 7.3 | 6.2 | 5.8 | – | 4.7 | 5.1 | 4.4 | 4.6 | 8.3 | 8.2 | 7.2 | 8.5 | 9.5 | 8.5 | 7.4 | 7.3 | 8.0 |
Std Deviation (1e-4) | 0.7 | 1.1 | 1.2 | 1.8 | 1.6 | 2.3 | 1.8 | – | 1.1 | 1.7 | 1.6 | 1.5 | 1.4 | 1.7 | 1.3 | 1.4 | 2.0 | 1.7 | 1.1 | 1.2 | 1.6 |
Intensity Change (Log2) | – | – | – | 0.03 | -0.04 | -0.26 | -0.37 | – | -0.66 | -0.54 | -0.77 | -0.7 | 0.16 | 0.13 | -0.06 | 0.18 | 0.34 | 0.19 | -0.01 | -0.03 | 0.09 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0.5 | -0.9 | 0 | 0 | – | 0 | 0 | 0 | 0 | 1.0 | 0 | 0 | 0.1 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | -0.7 | 2.9 | 3.9 | 5.1 | – | 2.2 | 2.4 | -1.4 | 0.1 | -4.5 | -3.0 | -1.4 | -3.8 | -4.9 | -4.5 |
Endoplasmic Reticulum | -0.8 | -3.1 | -3.7 | -6.0 | – | 0 | -6.9 | 0 | 0 | 1.9 | -1.8 | -3.0 | -4.9 | -5.2 | -4.6 |
Endosome | 1.8 | -0.5 | -0.6 | -2.0 | – | 0 | -3.7 | 0 | 0 | 1.9 | 5.1 | 4.4 | 4.0 | 5.6 | 6.4 |
Golgi | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 0 | 3.0 | – | 0 | 5.3 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 3.3 | 2.9 | 0 | 3.3 | 3.0 | 3.3 |
Vacuole | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0.9 | 1.1 | 1.7 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 2.3255 | 3.5745 | 2.8705 | 2.5065 | 2.5836 | 3.3056 | 2.7665 | 3.9676 | 3.6213 | 3.3332 | 3.1562 | 3.5994 | 3.9006 | 5.3202 | 4.4827 | 4.4032 | 4.1636 | 4.5308 |
Actin | 0.0234 | 0.0021 | 0.0384 | 0.029 | 0.003 | 0.0342 | 0.0088 | 0.0093 | 0.0097 | 0.0093 | 0.0106 | 0.0047 | 0.0035 | 0.0033 | 0.0033 | 0.0018 | 0.001 | 0.0009 |
Bud | 0.0004 | 0.0001 | 0.0007 | 0.0009 | 0.0003 | 0.0002 | 0.0003 | 0.0002 | 0.0004 | 0.0004 | 0.0004 | 0.0001 | 0.0001 | 0.0002 | 0.0001 | 0.0003 | 0 | 0.0001 |
Bud Neck | 0.0023 | 0.0014 | 0.0026 | 0.0016 | 0.0007 | 0.0012 | 0.0009 | 0.001 | 0.0046 | 0.0028 | 0.0009 | 0.0008 | 0.0003 | 0.0006 | 0.0007 | 0.0004 | 0.0003 | 0.0004 |
Bud Periphery | 0.0006 | 0.0001 | 0.0006 | 0.001 | 0.0006 | 0.0002 | 0.0005 | 0.0002 | 0.0003 | 0.0005 | 0.0005 | 0.0003 | 0.0001 | 0.0002 | 0.0001 | 0.001 | 0 | 0.0001 |
Bud Site | 0.0054 | 0.0031 | 0.016 | 0.0044 | 0.0005 | 0.0006 | 0.0047 | 0.0095 | 0.0045 | 0.0023 | 0.0013 | 0.0003 | 0.0003 | 0.008 | 0.0016 | 0.0002 | 0.0002 | 0.0002 |
Cell Periphery | 0.0006 | 0.0003 | 0.0005 | 0.0004 | 0.0003 | 0.0003 | 0.0003 | 0.0008 | 0.0004 | 0.0002 | 0.0002 | 0.0002 | 0.0001 | 0.0002 | 0.0002 | 0.0001 | 0.0001 | 0.0001 |
Cytoplasm | 0.0067 | 0.0767 | 0.0219 | 0.0056 | 0.0023 | 0.0045 | 0.0062 | 0.013 | 0.0214 | 0.0069 | 0.0016 | 0.0051 | 0.0063 | 0.0114 | 0.0131 | 0.0375 | 0.0032 | 0.0054 |
Cytoplasmic Foci | 0.1634 | 0.1561 | 0.179 | 0.1022 | 0.0993 | 0.0972 | 0.1343 | 0.1458 | 0.1555 | 0.1875 | 0.1183 | 0.1222 | 0.1179 | 0.1175 | 0.1346 | 0.1107 | 0.1483 | 0.1058 |
Eisosomes | 0.0002 | 0.0002 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0002 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0 | 0.0001 | 0 | 0 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0.0142 | 0.0197 | 0.008 | 0.0776 | 0.0067 | 0.0243 | 0.0041 | 0.0168 | 0.0127 | 0.0019 | 0.0013 | 0.0089 | 0.0129 | 0.0179 | 0.0083 | 0.0109 | 0.0017 | 0.0117 |
Endosome | 0.1989 | 0.2562 | 0.1911 | 0.3465 | 0.2639 | 0.1983 | 0.2011 | 0.2085 | 0.2106 | 0.2277 | 0.1823 | 0.1931 | 0.2889 | 0.2603 | 0.3017 | 0.3019 | 0.2803 | 0.2291 |
Golgi | 0.0998 | 0.0971 | 0.1319 | 0.1263 | 0.1916 | 0.1256 | 0.0918 | 0.083 | 0.1057 | 0.1392 | 0.1218 | 0.1096 | 0.0885 | 0.1117 | 0.1411 | 0.1649 | 0.1846 | 0.1024 |
Lipid Particles | 0.4004 | 0.3238 | 0.3437 | 0.2343 | 0.1768 | 0.4779 | 0.4712 | 0.4609 | 0.423 | 0.3217 | 0.4503 | 0.5085 | 0.4446 | 0.4356 | 0.3573 | 0.3129 | 0.3111 | 0.5094 |
Mitochondria | 0.0144 | 0.0026 | 0.0196 | 0.0402 | 0.1326 | 0.0025 | 0.0126 | 0.0134 | 0.0048 | 0.0313 | 0.0528 | 0.0059 | 0.0049 | 0.0031 | 0.0034 | 0.0036 | 0.0017 | 0.0037 |
None | 0.0062 | 0.016 | 0.0011 | 0.0002 | 0.0006 | 0.0002 | 0.0162 | 0.0017 | 0.0086 | 0.0003 | 0.0002 | 0.0012 | 0.0005 | 0.0007 | 0.0002 | 0.0016 | 0.0001 | 0.0003 |
Nuclear Periphery | 0.0063 | 0.0038 | 0.0013 | 0.003 | 0.0215 | 0.0086 | 0.0052 | 0.0014 | 0.0019 | 0.0006 | 0.001 | 0.0056 | 0.0083 | 0.0076 | 0.0052 | 0.0089 | 0.0005 | 0.0033 |
Nucleolus | 0.0006 | 0.0011 | 0.0001 | 0.0002 | 0.0002 | 0.0001 | 0.0005 | 0.0004 | 0.0004 | 0.0005 | 0.0002 | 0.0001 | 0.0002 | 0.0005 | 0.0001 | 0.0001 | 0.0001 | 0.0002 |
Nucleus | 0.0009 | 0.0019 | 0.0002 | 0.0003 | 0.0013 | 0.0002 | 0.0009 | 0.0005 | 0.0006 | 0.0002 | 0.0002 | 0.0002 | 0.0005 | 0.0006 | 0.0007 | 0.0008 | 0.0001 | 0.0003 |
Peroxisomes | 0.0363 | 0.0254 | 0.0359 | 0.01 | 0.0668 | 0.0127 | 0.0258 | 0.0211 | 0.0247 | 0.0598 | 0.0504 | 0.0261 | 0.0125 | 0.0096 | 0.0173 | 0.0143 | 0.061 | 0.0174 |
Punctate Nuclear | 0.0056 | 0.0065 | 0.0018 | 0.0007 | 0.0008 | 0.0015 | 0.0085 | 0.0019 | 0.0021 | 0.0018 | 0.001 | 0.0019 | 0.0025 | 0.0017 | 0.0039 | 0.0017 | 0.0008 | 0.0021 |
Vacuole | 0.0082 | 0.0043 | 0.0044 | 0.0116 | 0.0116 | 0.007 | 0.0037 | 0.0096 | 0.0065 | 0.0033 | 0.0025 | 0.0034 | 0.0051 | 0.0073 | 0.0054 | 0.0151 | 0.0035 | 0.0053 |
Vacuole Periphery | 0.0053 | 0.0014 | 0.0013 | 0.0039 | 0.0185 | 0.0028 | 0.0023 | 0.0009 | 0.0015 | 0.0018 | 0.0022 | 0.0016 | 0.0017 | 0.0019 | 0.0015 | 0.0114 | 0.0013 | 0.0017 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 27.3147 | 21.2748 | 22.245 | 27.091 | 22.7916 | 21.6234 | 23.222 | 28.2119 | 32.9837 | 29.0437 |
Translational Efficiency | 1.2277 | 1.412 | 1.147 | 1.2542 | 1.2645 | 1.3958 | 1.3748 | 1.1087 | 1.0358 | 1.1431 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✔ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
1471 | 2301 | 220 | 916 | 117 | 2189 | 1092 | 2773 | 1588 | 4490 | 1312 | 3689 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 712.15 | 859.14 | 1175.71 | 1127.52 | 847.00 | 840.42 | 1168.23 | 1258.89 | 722.09 | 850.01 | 1169.48 | 1226.27 |
Standard Deviation | 77.27 | 119.79 | 127.14 | 173.19 | 93.24 | 110.42 | 136.35 | 168.41 | 86.10 | 115.69 | 134.88 | 178.85 |
Intensity Change Log 2 | — | 0.270712 | 0.723279 | 0.662900 | — | -0.011251 | 0.463890 | 0.571718 | — | 0.124402 | 0.588176 | 0.614082 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.002376 | 0.017340 | 0.106418* | 0.070760 | 0.011262 | 0.006613 | 0.060716 | 0.094766 | 0.003031 | 0.012110 | 0.068380 | 0.088805 |
Bud Neck | 0.008846 | 0.001699 | 0.008166 | 0.005539 | 0.003979 | 0.001867 | 0.001641 | 0.003368 | 0.008487 | 0.001781 | 0.002735 | 0.003907 |
Bud Site | 0.002410 | 0.006299 | 0.021683 | 0.037248 | 0.005154 | 0.002493 | 0.010963 | 0.019094 | 0.002612 | 0.004444 | 0.012760 | 0.023601 |
Cell Periphery | 0.000383 | 0.000122 | 0.001458 | 0.002153 | 0.000981 | 0.000102 | 0.000377 | 0.000875 | 0.000427 | 0.000112 | 0.000558 | 0.001192 |
Cytoplasm | 0.103642* | 0.013565 | 0.003166 | 0.024099 | 0.073057 | 0.014524 | 0.011131 | 0.023800 | 0.101388* | 0.014033 | 0.009795 | 0.023874 |
Cytoplasmic Foci | 0.337106* | 0.213809* | 0.055343 | 0.189552* | 0.237792* | 0.247552* | 0.161139 | 0.166486 | 0.329789* | 0.230260* | 0.143399 | 0.172213 |
Eisosomes | 0.001714 | 0.000439 | 0.000576 | 0.001063 | 0.001440 | 0.000344 | 0.000639 | 0.002182 | 0.001694 | 0.000393 | 0.000628 | 0.001904 |
Endoplasmic Reticulum | 0.009391 | 0.005500 | 0.135338* | 0.047387 | 0.027021 | 0.005348 | 0.063839 | 0.016240 | 0.010690 | 0.005426 | 0.075828 | 0.023974 |
Endosome | 0.068180 | 0.129921 | 0.201699* | 0.226036* | 0.122553 | 0.171570 | 0.180087 | 0.186169 | 0.072186 | 0.150226 | 0.183711 | 0.196068 |
Golgi | 0.117623* | 0.282778* | 0.036244 | 0.110821* | 0.140649* | 0.269828* | 0.139489* | 0.245827* | 0.119320* | 0.276465* | 0.122177* | 0.212304* |
Lipid Particles | 0.244761* | 0.277434* | 0.369421* | 0.162958* | 0.286368* | 0.217708* | 0.317645* | 0.182317* | 0.247827* | 0.248316* | 0.326327* | 0.177510* |
Mitochondria | 0.021077 | 0.011390 | 0.000101 | 0.001562 | 0.003909 | 0.015006 | 0.000346 | 0.006975 | 0.019813 | 0.013153 | 0.000305 | 0.005631 |
Mitotic Spindle | 0.000952 | 0.006359 | 0.005526 | 0.037240 | 0.000715 | 0.008722 | 0.012966 | 0.007910 | 0.000934 | 0.007511 | 0.011718 | 0.015193 |
None | 0.001451 | 0.000326 | 0.000005 | 0.000132 | 0.000197 | 0.000198 | 0.000041 | 0.000073 | 0.001359 | 0.000264 | 0.000035 | 0.000088 |
Nuclear Periphery | 0.000916 | 0.000129 | 0.000423 | 0.000511 | 0.000906 | 0.000286 | 0.000233 | 0.000113 | 0.000915 | 0.000206 | 0.000265 | 0.000212 |
Nuclear Periphery Foci | 0.001662 | 0.002631 | 0.027188 | 0.011977 | 0.006470 | 0.002710 | 0.015886 | 0.002185 | 0.002016 | 0.002669 | 0.017781 | 0.004617 |
Nucleolus | 0.001324 | 0.000238 | 0.000008 | 0.000191 | 0.000551 | 0.000173 | 0.000048 | 0.000028 | 0.001267 | 0.000206 | 0.000041 | 0.000069 |
Nucleus | 0.011219 | 0.000916 | 0.000123 | 0.001209 | 0.006881 | 0.000551 | 0.000346 | 0.000375 | 0.010900 | 0.000738 | 0.000309 | 0.000582 |
Peroxisomes | 0.046811 | 0.020879 | 0.004263 | 0.010249 | 0.044375 | 0.017620 | 0.005725 | 0.016011 | 0.046632 | 0.019290 | 0.005480 | 0.014580 |
Vacuole | 0.015626 | 0.005993 | 0.022065 | 0.055044 | 0.021728 | 0.012423 | 0.015881 | 0.021978 | 0.016075 | 0.009128 | 0.016918 | 0.030188 |
Vacuole Periphery | 0.002530 | 0.002232 | 0.000787 | 0.004267 | 0.004012 | 0.004362 | 0.000863 | 0.003229 | 0.002639 | 0.003270 | 0.000851 | 0.003487 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -9.25 | -7.24 | -11.32 | -8.49 | 2.39 | 0.86 | -6.90 | -12.80 | -22.71* | -5.56 | -8.71 | -13.85 | -26.40 | -22.89 | -3.52 |
Bud Neck | 9.65 | 0.45 | 3.19 | -5.73 | 1.74 | 2.39 | 2.59 | 0.51 | -3.80 | -4.40 | 9.97 | 8.04 | 6.16 | -6.41 | -3.03 |
Bud Site | -4.00 | -4.98 | -10.93 | -9.39 | -3.26 | 1.57 | -2.81 | -7.15 | -14.39 | -4.95 | -3.21 | -7.95 | -17.62 | -15.29 | -6.56 |
Cell Periphery | 5.59 | -2.82 | -5.03 | -5.83 | -1.29 | 3.10 | 2.11 | 0.36 | -9.68 | -5.85 | 6.60 | -1.61 | -6.64 | -10.27 | -4.99 |
Cytoplasm | 21.28 | 23.64 | 14.58 | -4.09 | -7.12 | 4.47 | 4.65 | 3.68 | -5.52 | -6.38 | 21.89 | 22.28 | 18.34 | -7.28 | -8.38 |
Cytoplasmic Foci | 18.60 | 32.36 | 18.83 | 3.87 | -15.43 | -0.43 | 4.64 | 4.41 | 17.94 | -1.08 | 16.71 | 27.62 | 26.65 | 16.64 | -6.06 |
Eisosomes | 7.04 | 5.88 | 2.82 | -3.78 | -2.69 | 2.07 | 1.50 | -1.32 | -11.04 | -8.40 | 7.60 | 5.81 | -0.92 | -11.48 | -8.63 |
Endoplasmic Reticulum | 3.01 | -8.45 | -7.93 | -8.95 | 5.72 | 1.99 | -3.24 | 0.96 | -8.22 | 10.36 | 3.74 | -13.88 | -6.72 | -12.18 | 11.01 |
Endosome | -15.25 | -11.60 | -20.99 | -12.35 | -1.60 | -3.53 | -3.98 | -4.56 | -2.77 | -1.04 | -21.62 | -21.13 | -30.03 | -11.37 | -2.14 |
Golgi | -26.40* | 14.23 | 1.49 | 25.95 | -11.59 | -8.03 | 0.10 | -6.49 | 4.08 | -17.34 | -30.36* | -0.44 | -17.45 | 13.62 | -17.02 |
Lipid Particles | -3.70 | -6.16 | 8.42 | 11.98 | 10.07 | 2.48 | -1.12 | 3.81 | 4.78 | 14.14 | -0.07 | -7.85 | 9.58 | 12.35 | 17.23 |
Mitochondria | 5.08 | 12.80 | 11.68 | 9.91 | -4.48 | -7.06 | 3.07 | -2.20 | 6.09 | -8.50 | 3.92 | 12.78 | 8.69 | 8.20 | -8.97 |
Mitotic Spindle | -7.09 | -2.86 | -8.56 | -7.27 | -7.11 | -7.83 | -6.18 | -7.94 | 0.40 | 2.26 | -9.73 | -6.39 | -11.02 | -5.73 | -1.85 |
None | 3.14 | 4.11 | 3.69 | 1.94 | -3.69 | 0.03 | 1.46 | 1.19 | 1.35 | -1.87 | 3.32 | 4.05 | 3.89 | 2.72 | -3.45 |
Nuclear Periphery | 3.98 | 2.27 | 1.74 | -3.57 | -0.72 | 1.32 | 1.41 | 1.69 | 3.01 | 3.14 | 3.79 | 3.40 | 3.70 | -0.39 | 1.21 |
Nuclear Periphery Foci | -2.48 | -5.06 | -6.95 | -6.24 | 2.74 | 1.98 | -3.91 | 2.21 | 0.71 | 8.26 | -1.86 | -9.86 | -5.50 | -4.04 | 7.85 |
Nucleolus | 3.14 | 3.88 | 3.19 | 0.16 | -3.98 | 1.24 | 1.65 | 1.75 | 5.19 | 0.67 | 3.34 | 3.84 | 3.76 | 3.43 | -0.85 |
Nucleus | 13.80 | 15.24 | 12.65 | -1.64 | -4.99 | 3.28 | 3.30 | 3.31 | 0.64 | 0.08 | 14.62 | 14.98 | 14.61 | 0.25 | -1.81 |
Peroxisomes | 9.99 | 18.27 | 15.13 | 6.76 | -5.60 | 4.76 | 6.97 | 5.11 | 1.23 | -9.17 | 11.94 | 18.73 | 14.40 | 4.34 | -9.98 |
Vacuole | 7.40 | -1.98 | -10.03 | -12.68 | -7.01 | 2.12 | 1.12 | -0.49 | -6.75 | -3.59 | 5.40 | -0.88 | -8.85 | -14.89 | -7.47 |
Vacuole Periphery | 1.02 | 6.46 | -2.19 | -2.52 | -4.43 | -0.19 | 1.61 | 0.39 | 2.05 | -6.86 | -1.98 | 7.91 | -2.20 | -0.48 | -8.16 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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