Standard name | |
---|---|
Human Ortholog | |
Description | Plasma membrane protein involved in remodeling GPI anchors; member of the MBOAT family of putative membrane-bound O-acyltransferases; role in misfolded protein quality control; proposed to be involved in glycerol transport; homolog of the mammalian Hedgehog pathway modulator HHATL; GUP1 has a paralog, GUP2, that arose from the whole genome duplication |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 1 | 2 | 15 | 15 | 11 | 9 | 1 | 14 | 3 | 6 | 4 | 5 | 8 | 7 | 9 | 1 | 0 | 0 | 0 | 2 | 5 | 3 | 4 | 6 |
Bud | 0 | 1 | 1 | 2 | 5 | 3 | 3 | 4 | 11 | 18 | 14 | 11 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 2 | 1 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 1 | 1 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 1 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 4 | 2 | 10 | 11 | 27 | 17 | 17 | 20 | 17 | 18 | 20 | 17 | 33 | 33 | 55 | 3 | 4 | 0 | 0 | 0 | 0 | 0 | 2 | 4 |
Cytoplasm | 35 | 41 | 36 | 31 | 92 | 64 | 95 | 82 | 88 | 91 | 80 | 77 | 54 | 54 | 75 | 11 | 13 | 15 | 11 | 15 | 1 | 4 | 17 | 15 |
Endoplasmic Reticulum | 166 | 95 | 161 | 181 | 112 | 140 | 125 | 165 | 86 | 65 | 68 | 67 | 311 | 297 | 366 | 0 | 0 | 1 | 199 | 117 | 234 | 192 | 320 | 385 |
Endosome | 0 | 0 | 11 | 7 | 15 | 2 | 17 | 14 | 10 | 9 | 3 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 1 | 1 | 2 | 2 |
Golgi | 0 | 0 | 0 | 3 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 2 | 1 | 3 |
Mitochondria | 0 | 2 | 7 | 36 | 25 | 50 | 78 | 80 | 94 | 157 | 114 | 129 | 0 | 0 | 0 | 1 | 0 | 3 | 2 | 1 | 1 | 5 | 10 | 10 |
Nucleus | 0 | 0 | 0 | 0 | 1 | 0 | 2 | 3 | 2 | 1 | 3 | 0 | 2 | 2 | 3 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 5 | 4 | 7 | 9 | 5 | 5 | 10 | 14 | 14 | 9 | 11 | 14 | 1 | 1 | 2 | 0 | 0 | 0 | 2 | 1 | 1 | 1 | 1 | 1 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 1 | 2 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vac/Vac Membrane | 9 | 2 | 19 | 11 | 99 | 33 | 99 | 76 | 68 | 57 | 22 | 60 | 4 | 3 | 13 | 0 | 1 | 3 | 2 | 5 | 4 | 6 | 15 | 21 |
Unique Cell Count | 202 | 134 | 220 | 240 | 327 | 259 | 370 | 388 | 308 | 341 | 263 | 300 | 378 | 366 | 467 | 16 | 17 | 23 | 222 | 152 | 256 | 221 | 383 | 461 |
Labelled Cell Count | 220 | 149 | 267 | 306 | 394 | 325 | 449 | 475 | 394 | 432 | 339 | 383 | 414 | 398 | 524 | 16 | 18 | 23 | 222 | 152 | 256 | 221 | 383 | 461 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 8.7 | 8.4 | 8.6 | 8.9 | 9.3 | 8.2 | 7.6 | 7.7 | 7.2 | 6.4 | 6.8 | 6.8 | 10.6 | 10.6 | 10.8 | 7.8 | 8.3 | 9.9 | 9.7 | 10.1 | 10.7 |
Std Deviation (1e-4) | 1.1 | 1.4 | 1.5 | 1.5 | 1.8 | 1.5 | 1.3 | 1.4 | 1.5 | 1.3 | 1.6 | 1.3 | 1.3 | 1.3 | 1.2 | 1.0 | 1.5 | 1.6 | 1.3 | 1.4 | 1.6 |
Intensity Change (Log2) | – | – | – | 0.04 | 0.11 | -0.07 | -0.18 | -0.17 | -0.26 | -0.43 | -0.35 | -0.34 | 0.3 | 0.3 | 0.33 | -0.15 | -0.05 | 0.2 | 0.16 | 0.22 | 0.31 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | -0.2 | -1.9 | -1.7 | -4.7 | -1.8 | -3.6 | -3.1 | -3.0 | -3.0 | -2.9 | -3.0 | -3.3 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 2.4 | 3.1 | 3.1 | 2.4 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 1.7 | 1.0 | 0 | 0.3 | 0.5 | 0.4 | 1.4 | 0.6 | 1.9 | 2.0 | 3.0 | 0 | 0 | 0 |
Cytoplasm | -1.0 | 3.2 | 2.2 | 2.6 | 1.4 | 3.3 | 2.9 | 3.6 | 2.5 | -0.7 | -0.5 | -0.1 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0.5 | -8.9 | -4.3 | -9.3 | -7.3 | -10.3 | -12.8 | -10.4 | -11.5 | 2.6 | 2.3 | 1.5 | -6.1 | -6.2 | -6.7 |
Endosome | -1.2 | -0.2 | -2.8 | -0.2 | -0.8 | -1.0 | -1.5 | -2.5 | -3.1 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 4.3 | 2.2 | 5.4 | 6.0 | 5.9 | 7.9 | 10.9 | 10.1 | 10.2 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0.3 | 0 | -0.9 | -0.3 | 0.3 | 0.8 | -0.4 | 0.6 | 0.8 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | -1.8 | 6.0 | 1.4 | 5.3 | 3.6 | 4.1 | 2.7 | -0.1 | 3.6 | -4.6 | -4.8 | -3.4 | 0 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 5.7981 | 6.6116 | 6.002 | 5.3521 | 5.9277 | 5.8753 | 7.3815 | 8.3098 | 7.5518 | 6.62 | 7.516 | 8.1147 | 7.4696 | 8.1898 | 7.4477 | 7.2327 | 7.3168 | 7.1608 |
Actin | 0.0445 | 0.0017 | 0.0062 | 0.0005 | 0.0001 | 0.0006 | 0.0046 | 0.0043 | 0.009 | 0.0001 | 0.0002 | 0.0017 | 0.0353 | 0.0003 | 0.0001 | 0.024 | 0.0003 | 0.0002 |
Bud | 0.0005 | 0.0005 | 0.0002 | 0 | 0 | 0 | 0 | 0 | 0.0001 | 0 | 0.0001 | 0 | 0.0001 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0.0208 | 0.0003 | 0.0005 | 0.0001 | 0.0001 | 0.0005 | 0.0002 | 0.0001 | 0.0003 | 0.0001 | 0.0001 | 0.0001 | 0.0006 | 0.0001 | 0 | 0.0007 | 0.0003 | 0.0001 |
Bud Periphery | 0.0007 | 0.0004 | 0.0002 | 0 | 0 | 0 | 0.0001 | 0 | 0.0002 | 0 | 0 | 0.0001 | 0.0002 | 0 | 0 | 0.0001 | 0 | 0 |
Bud Site | 0.0041 | 0.003 | 0.0022 | 0 | 0 | 0 | 0.0002 | 0.0006 | 0.0014 | 0 | 0.0006 | 0.0005 | 0.0003 | 0.0001 | 0 | 0.0002 | 0 | 0 |
Cell Periphery | 0.0008 | 0.0017 | 0.0006 | 0.0002 | 0 | 0.0002 | 0.0011 | 0.0007 | 0.0008 | 0.0004 | 0.0001 | 0.0002 | 0.0009 | 0.001 | 0.0003 | 0.0003 | 0.0002 | 0.0003 |
Cytoplasm | 0.0214 | 0.1465 | 0.098 | 0.047 | 0.0584 | 0.0595 | 0.0225 | 0.0777 | 0.0542 | 0.0475 | 0.0225 | 0.0423 | 0.1067 | 0.0546 | 0.021 | 0.003 | 0.0368 | 0.0252 |
Cytoplasmic Foci | 0.0121 | 0.0029 | 0.0071 | 0.0029 | 0.001 | 0.0026 | 0.0049 | 0.0042 | 0.0015 | 0.0005 | 0.0486 | 0.0028 | 0.0012 | 0.0011 | 0.0003 | 0.004 | 0.0006 | 0.0003 |
Eisosomes | 0.0015 | 0.0001 | 0.0004 | 0 | 0 | 0 | 0.0001 | 0 | 0.0007 | 0 | 0 | 0 | 0.0022 | 0 | 0 | 0.0003 | 0 | 0 |
Endoplasmic Reticulum | 0.7611 | 0.7994 | 0.8136 | 0.8523 | 0.9 | 0.8957 | 0.9128 | 0.8687 | 0.8872 | 0.9366 | 0.8934 | 0.9285 | 0.8093 | 0.9192 | 0.9693 | 0.9301 | 0.9474 | 0.9532 |
Endosome | 0.0367 | 0.0097 | 0.0185 | 0.0146 | 0.0069 | 0.0087 | 0.0183 | 0.0144 | 0.008 | 0.0023 | 0.0151 | 0.0045 | 0.0023 | 0.0028 | 0.0016 | 0.0092 | 0.0037 | 0.0045 |
Golgi | 0.013 | 0.0015 | 0.0033 | 0.0013 | 0.0002 | 0.0007 | 0.0024 | 0.0042 | 0.0032 | 0.0001 | 0.0036 | 0.0013 | 0.0028 | 0.0003 | 0.0003 | 0.0066 | 0.0003 | 0.0002 |
Lipid Particles | 0.0102 | 0.0067 | 0.0068 | 0.0117 | 0.0004 | 0.0024 | 0.0138 | 0.0064 | 0.0086 | 0.0007 | 0.0028 | 0.0056 | 0.0013 | 0.0039 | 0.0014 | 0.0105 | 0.0012 | 0.0008 |
Mitochondria | 0.0137 | 0.0007 | 0.0095 | 0.0003 | 0.0001 | 0.0001 | 0.0007 | 0.0002 | 0.0045 | 0 | 0.0002 | 0.0008 | 0.0003 | 0 | 0 | 0.0004 | 0.0001 | 0.0004 |
None | 0.0027 | 0.0013 | 0.0093 | 0.0005 | 0.0003 | 0.0021 | 0.0016 | 0.0007 | 0.0064 | 0.0002 | 0.0002 | 0.0002 | 0.0185 | 0.0028 | 0.0001 | 0.0011 | 0.0001 | 0.0009 |
Nuclear Periphery | 0.0259 | 0.014 | 0.0141 | 0.0636 | 0.0271 | 0.0221 | 0.0117 | 0.0092 | 0.0061 | 0.0061 | 0.0046 | 0.0061 | 0.0146 | 0.0051 | 0.004 | 0.0057 | 0.0059 | 0.009 |
Nucleolus | 0.0016 | 0.0002 | 0.0004 | 0 | 0 | 0 | 0.0001 | 0.0001 | 0.0001 | 0 | 0 | 0 | 0 | 0 | 0 | 0.0001 | 0 | 0 |
Nucleus | 0.0104 | 0.0027 | 0.0019 | 0.0011 | 0.0024 | 0.0023 | 0.0008 | 0.0012 | 0.0021 | 0.0005 | 0.0004 | 0.0005 | 0.0014 | 0.0004 | 0.0002 | 0.0003 | 0.0005 | 0.0005 |
Peroxisomes | 0.0055 | 0.0001 | 0.0023 | 0.0001 | 0 | 0 | 0.0004 | 0 | 0.0002 | 0 | 0.0048 | 0.0027 | 0.0001 | 0 | 0 | 0.001 | 0 | 0 |
Punctate Nuclear | 0.0077 | 0.0006 | 0.0016 | 0.0008 | 0.0001 | 0.0003 | 0.0012 | 0.0005 | 0.0001 | 0.0001 | 0.0013 | 0.0002 | 0.0002 | 0.0002 | 0 | 0.0013 | 0.0001 | 0 |
Vacuole | 0.0037 | 0.0055 | 0.0028 | 0.0017 | 0.0024 | 0.0017 | 0.0019 | 0.006 | 0.0046 | 0.0044 | 0.0012 | 0.0016 | 0.0013 | 0.0081 | 0.0012 | 0.0013 | 0.0024 | 0.0037 |
Vacuole Periphery | 0.0015 | 0.0005 | 0.0007 | 0.0013 | 0.0004 | 0.0005 | 0.0006 | 0.0005 | 0.0006 | 0.0002 | 0.0002 | 0.0002 | 0.0005 | 0.0001 | 0.0001 | 0.0003 | 0.0002 | 0.0005 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 28.8472 | 21.099 | 28.7033 | 34.2217 | 22.526 | 35.3838 | 37.1848 | 43.3953 | 38.1012 | 45.4172 |
Translational Efficiency | 0.7916 | 1.0694 | 0.8145 | 0.5634 | 0.8074 | 0.9407 | 0.8166 | 0.6504 | 0.7891 | 0.7418 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
111 | 1054 | 141 | 175 | 1150 | 2206 | 532 | 1585 | 1261 | 3260 | 673 | 1760 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 1116.65 | 951.37 | 1409.63 | 1351.76 | 968.84 | 994.84 | 1140.60 | 1319.92 | 981.85 | 980.79 | 1196.96 | 1323.09 |
Standard Deviation | 146.54 | 153.55 | 174.19 | 227.99 | 137.06 | 147.56 | 295.43 | 204.36 | 144.14 | 150.90 | 295.53 | 207.05 |
Intensity Change Log 2 | — | -0.231099 | 0.336139 | 0.275662 | — | 0.038206 | 0.235463 | 0.446120 | — | -0.099719 | 0.290241 | 0.357361 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.000991 | 0.001026 | 0.000149 | 0.001845 | 0.000602 | 0.000156 | 0.003557 | 0.001244 | 0.000636 | 0.000437 | 0.002843 | 0.001303 |
Bud Neck | 0.002876 | 0.000700 | 0.001391 | 0.003066 | 0.000375 | 0.000353 | 0.001981 | 0.001636 | 0.000595 | 0.000465 | 0.001857 | 0.001778 |
Bud Site | 0.016780 | 0.005617 | 0.001014 | 0.008060 | 0.003135 | 0.000910 | 0.019415 | 0.007975 | 0.004336 | 0.002432 | 0.015560 | 0.007983 |
Cell Periphery | 0.000681 | 0.000725 | 0.000368 | 0.004119 | 0.001680 | 0.000537 | 0.001392 | 0.003522 | 0.001592 | 0.000598 | 0.001177 | 0.003581 |
Cytoplasm | 0.090891 | 0.080172 | 0.024044 | 0.021064 | 0.029143 | 0.065548 | 0.077767 | 0.025910 | 0.034579 | 0.070276 | 0.066511 | 0.025428 |
Cytoplasmic Foci | 0.067718 | 0.025905 | 0.002562 | 0.004119 | 0.007473 | 0.009714 | 0.023006 | 0.002876 | 0.012777 | 0.014948 | 0.018723 | 0.003000 |
Eisosomes | 0.000171 | 0.000285 | 0.000007 | 0.000360 | 0.000203 | 0.000144 | 0.000038 | 0.000210 | 0.000200 | 0.000190 | 0.000031 | 0.000225 |
Endoplasmic Reticulum | 0.211512* | 0.788269* | 0.757581* | 0.803692* | 0.922159* | 0.871548* | 0.731377* | 0.908079* | 0.859604* | 0.844623* | 0.736867* | 0.897700* |
Endosome | 0.045590 | 0.011523 | 0.018032 | 0.010749 | 0.004005 | 0.004178 | 0.021810 | 0.007863 | 0.007665 | 0.006553 | 0.021018 | 0.008150 |
Golgi | 0.022452 | 0.009018 | 0.000760 | 0.001268 | 0.004742 | 0.006371 | 0.008228 | 0.003821 | 0.006301 | 0.007227 | 0.006663 | 0.003567 |
Lipid Particles | 0.031672 | 0.020034 | 0.006541 | 0.016454 | 0.006563 | 0.010861 | 0.013446 | 0.011884 | 0.008774 | 0.013827 | 0.012000 | 0.012338 |
Mitochondria | 0.009105 | 0.002951 | 0.000079 | 0.000291 | 0.002886 | 0.004160 | 0.000978 | 0.001556 | 0.003433 | 0.003769 | 0.000790 | 0.001430 |
Mitotic Spindle | 0.016530 | 0.000265 | 0.000874 | 0.000322 | 0.000091 | 0.000733 | 0.001305 | 0.000218 | 0.001538 | 0.000582 | 0.001215 | 0.000228 |
None | 0.000038 | 0.002906 | 0.000061 | 0.002100 | 0.002411 | 0.004110 | 0.002446 | 0.002093 | 0.002203 | 0.003721 | 0.001946 | 0.002094 |
Nuclear Periphery | 0.010140 | 0.007270 | 0.026279 | 0.021780 | 0.001567 | 0.003039 | 0.005177 | 0.000388 | 0.002321 | 0.004407 | 0.009598 | 0.002515 |
Nuclear Periphery Foci | 0.046376 | 0.022264 | 0.114171 | 0.067476 | 0.006098 | 0.007331 | 0.052996 | 0.006820 | 0.009644 | 0.012159 | 0.065812 | 0.012851 |
Nucleolus | 0.000099 | 0.000198 | 0.000018 | 0.000062 | 0.000313 | 0.000182 | 0.000438 | 0.000030 | 0.000294 | 0.000187 | 0.000350 | 0.000033 |
Nucleus | 0.019359 | 0.004852 | 0.005664 | 0.004739 | 0.001447 | 0.004538 | 0.006949 | 0.001570 | 0.003024 | 0.004640 | 0.006680 | 0.001885 |
Peroxisomes | 0.001660 | 0.001254 | 0.000025 | 0.000226 | 0.001106 | 0.000692 | 0.000402 | 0.000295 | 0.001155 | 0.000874 | 0.000323 | 0.000288 |
Vacuole | 0.397003* | 0.011935 | 0.038997 | 0.027868 | 0.002491 | 0.003763 | 0.023961 | 0.009917 | 0.037218 | 0.006405 | 0.027111 | 0.011702 |
Vacuole Periphery | 0.008355 | 0.002832 | 0.001381 | 0.000341 | 0.001511 | 0.001132 | 0.003333 | 0.002094 | 0.002113 | 0.001682 | 0.002924 | 0.001919 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -0.25 | 2.55 | -1.75 | -1.51 | -3.11 | 2.31 | -2.02 | -2.08 | -3.97 | 1.51 | 0.90 | -1.91 | -2.41 | -3.27 | 1.23 |
Bud Neck | 5.27 | 3.12 | -0.42 | -1.60 | -1.21 | 0.19 | -3.79 | -3.88 | -3.98 | 0.49 | 1.17 | -3.69 | -3.64 | -4.03 | -0.10 |
Bud Site | 2.43 | 3.62 | 1.15 | -1.12 | -2.46 | 3.04 | -4.69 | -3.55 | -5.66 | 3.03 | 2.15 | -4.04 | -2.98 | -4.71 | 2.37 |
Cell Periphery | -0.48 | 1.43 | -2.04 | -1.98 | -2.18 | 1.71 | 0.41 | -2.37 | -6.70 | -4.55 | 1.63 | 0.65 | -2.77 | -6.86 | -5.36 |
Cytoplasm | 1.92 | 5.68 | 5.99 | 8.89 | 0.41 | -10.47 | -6.81 | 0.65 | 11.53 | 7.14 | -10.15 | -5.10 | 3.04 | 14.64 | 6.85 |
Cytoplasmic Foci | 4.36 | 7.25 | 6.92 | 7.38 | -0.99 | -1.59 | -4.93 | 3.85 | 6.35 | 6.65 | -1.52 | -2.34 | 6.75 | 10.67 | 6.45 |
Eisosomes | -0.70 | 5.63 | -0.86 | -0.29 | -1.50 | 0.95 | 2.95 | -0.14 | -1.11 | -3.07 | 0.12 | 3.29 | -0.40 | -0.49 | -3.48 |
Endoplasmic Reticulum | -21.27* | -16.45 | -17.89 | -0.29 | -1.34 | 7.12 | 11.88 | 2.43 | -4.67 | -10.81 | 1.37 | 8.08 | -3.82 | -7.23 | -11.40 |
Endosome | 5.23 | 3.71 | 5.24 | 0.36 | 2.36 | -0.32 | -9.55 | -5.27 | -5.28 | 7.26 | 0.89 | -8.17 | -1.11 | -2.47 | 7.80 |
Golgi | 1.17 | 2.16 | 2.10 | 7.75 | -1.28 | -1.61 | -2.45 | 0.89 | 2.39 | 3.01 | -1.10 | -0.52 | 2.02 | 4.04 | 2.57 |
Lipid Particles | 1.98 | 4.88 | 1.62 | 0.38 | -1.89 | -3.66 | -3.35 | -3.33 | -0.82 | 0.60 | -4.42 | -2.02 | -2.51 | 0.62 | -0.26 |
Mitochondria | 0.72 | 1.16 | 1.13 | 4.83 | -1.38 | -1.50 | 3.20 | 1.81 | 3.00 | -0.93 | -0.53 | 3.24 | 2.13 | 3.39 | -1.18 |
Mitotic Spindle | 2.07 | 2.01 | 2.06 | -0.33 | 0.78 | -2.04 | -2.72 | -2.41 | 1.59 | 2.40 | 1.48 | 0.74 | 1.89 | 1.57 | 2.56 |
None | -4.61 | 0.43 | -0.98 | 0.38 | -0.99 | -1.98 | -0.08 | 0.44 | 2.52 | 0.47 | -2.16 | 0.30 | 0.17 | 2.44 | -0.16 |
Nuclear Periphery | 0.70 | -3.28 | -2.15 | -2.68 | 0.67 | -2.44 | -2.61 | 2.46 | 7.03 | 3.67 | -3.22 | -4.83 | -0.36 | 2.70 | 4.61 |
Nuclear Periphery Foci | 3.16 | -4.87 | -1.94 | -4.63 | 2.96 | -1.07 | -9.28 | -0.87 | 0.11 | 9.11 | -1.92 | -11.40 | -2.18 | -0.70 | 10.58 |
Nucleolus | -1.12 | 4.70 | 1.49 | 1.69 | -1.55 | 0.54 | -0.51 | 1.24 | 1.92 | 3.93 | 0.50 | -0.26 | 1.26 | 2.60 | 3.85 |
Nucleus | 2.97 | 1.69 | 2.86 | -0.04 | 0.35 | -5.44 | -4.13 | -0.73 | 4.65 | 3.85 | -2.28 | -2.35 | 1.44 | 4.65 | 3.02 |
Peroxisomes | 0.48 | 3.90 | 3.26 | 2.80 | -2.13 | 2.73 | 4.33 | 5.87 | 3.87 | 0.93 | 1.52 | 5.66 | 6.53 | 4.23 | 0.36 |
Vacuole | 14.45 | 13.26 | 13.52 | -3.15 | 1.11 | -1.89 | -7.96 | -5.43 | -4.54 | 4.55 | 7.81 | 2.46 | 6.09 | -4.07 | 5.37 |
Vacuole Periphery | 1.55 | 2.04 | 2.45 | 6.71 | 1.98 | 0.98 | -2.22 | -0.97 | -1.79 | 1.36 | 0.76 | -1.27 | 0.13 | -0.50 | 1.31 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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