Standard name
Human Ortholog
Description Central kinetochore associated protein; mediates mitotic progression; interacts with several central kinetochore proteins and centromeric histone Cse4p; role in resistance to oxidative stress; similar to Slk19p; YBP2 has a paralog, YBP1, that arose from the whole genome duplication
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.99 0.99 0.99 0.99 0.97 0.93 0.92 0.87 0.73 0.77 0.75 0.77 0.97 0.99 1.0 0.9 0.92 0.91 0.93 0.94 0.94 0.95 0.94 0.92
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0.05 0 0.1 0.24 0.13 0.34 0.13 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0.06 0.1 0.11 0 0.06 0 0 0 0.12 0.09 0.13 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 2 0 1 0 0
Bud 1 2 0 2 0 1 1 1 3 0 0 0 0 0 1 0 1 0 2 1 1 0 0 1
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 1
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 1 6 8 4 2 2 4 4 3 4 4 8 3 2 2 9 8 6 0 0 0 0 0 0
Cytoplasm 303 443 248 209 257 272 291 246 138 204 126 135 217 289 248 195 171 186 284 425 235 185 236 257
Endoplasmic Reticulum 1 2 0 0 0 2 8 0 2 4 3 3 1 0 0 6 4 8 1 0 1 0 1 2
Endosome 0 0 0 1 2 0 0 2 1 1 0 0 1 1 0 0 0 1 5 5 1 0 0 2
Golgi 1 2 0 0 0 0 0 1 0 0 0 0 2 0 0 2 0 2 4 3 0 0 0 2
Mitochondria 0 2 0 4 0 14 14 28 45 35 56 23 0 0 0 5 2 3 1 3 1 2 5 1
Nucleus 0 1 4 1 0 2 9 5 3 4 3 10 1 4 0 4 5 3 0 2 0 0 0 0
Nuclear Periphery 0 1 0 0 0 0 0 0 1 1 0 0 0 0 0 0 0 1 0 0 0 0 0 0
Nucleolus 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 1 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 0 0 1 1
Vac/Vac Membrane 1 3 1 3 10 12 7 17 19 29 3 11 3 2 2 25 17 27 0 3 3 0 1 2
Unique Cell Count 307 449 250 212 265 293 315 282 189 264 167 176 223 292 249 216 186 205 306 453 249 195 251 278
Labelled Cell Count 309 462 261 224 271 305 334 304 215 282 195 190 228 298 253 247 208 238 306 453 249 195 251 278


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 6.4 7.8 7.9 7.5 7.9 6.9 6.8 7.2 5.9 6.4 5.6 6.1 8.3 8.4 8.0 11.3 11.1 10.0 7.9 8.2 8.4
Std Deviation (1e-4) 0.8 1.2 1.4 1.7 1.7 1.3 1.3 1.6 1.1 1.2 1.1 0.9 1.2 1.3 1.1 2.5 2.3 2.3 1.2 1.3 1.3
Intensity Change (Log2) – – – -0.08 0.01 -0.19 -0.21 -0.13 -0.42 -0.3 -0.5 -0.37 0.08 0.09 0.01 0.51 0.49 0.34 -0.0 0.05 0.1


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery -0.9 0 0 -1.6 -1.4 0 -1.3 0 0.7 -1.3 0 0 0.6 0.6 -0.2
Cytoplasm -0.6 -1.8 -3.7 -3.8 -5.3 -8.3 -7.6 -7.8 -7.6 -1.6 -0.3 0.6 -4.4 -3.9 -4.3
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 3.5 3.4 5.1 8.1 6.0 9.8 5.9 0 0 0 0 0 0
Nucleus 0 0 0 1.0 0 0 0 0 2.3 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 2.6 2.8 0 3.6 4.8 5.1 0 0 0 0 0 5.2 4.5 5.6

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 0.8009 2.0987 1.6822 1.3554 1.3347 1.2501 1.6792 2.3838 2.1245 1.9362 1.8522 2.157 1.6351 2.572 2.2295 2.2946 2.2926 2.0214
Actin 0.0288 0.0015 0.0042 0.0071 0.0311 0.0022 0.034 0.0003 0.0195 0.0044 0.0016 0.0102 0.0205 0.0004 0.0127 0.0003 0.0322 0.0126
Bud 0.0005 0.0054 0.0023 0.0004 0.0043 0.0008 0.0006 0.0002 0.0025 0.0013 0.0017 0.0043 0.0012 0.0003 0.0012 0.0003 0.0014 0.0003
Bud Neck 0.0015 0.0002 0.007 0.0006 0.0011 0.0009 0.0052 0.0002 0.001 0.0007 0.001 0.0017 0.0039 0.0001 0.0007 0.0005 0.0045 0.0011
Bud Periphery 0.0005 0.001 0.0007 0.0003 0.0037 0.0004 0.0007 0.0001 0.0012 0.002 0.0019 0.0029 0.0014 0.0001 0.0011 0.0003 0.0026 0.0003
Bud Site 0.0041 0.0098 0.0106 0.0008 0.0028 0.0001 0.0078 0.0015 0.0119 0.0044 0.0227 0.0011 0.0076 0.0011 0.0027 0.0002 0.0046 0.0002
Cell Periphery 0.0002 0.0002 0.0002 0.0001 0.0005 0.0001 0.0004 0.0002 0.0002 0.0003 0.0002 0.0001 0.0003 0 0.0001 0 0.0004 0
Cytoplasm 0.636 0.8679 0.8001 0.741 0.5987 0.8224 0.628 0.9051 0.7896 0.7773 0.6789 0.8372 0.7557 0.9522 0.8853 0.9158 0.7111 0.9014
Cytoplasmic Foci 0.0348 0.0088 0.0191 0.0244 0.0238 0.0202 0.0425 0.0082 0.0113 0.0272 0.0802 0.0109 0.0206 0.0046 0.0156 0.0063 0.052 0.0097
Eisosomes 0.0004 0.0001 0.0001 0.0001 0.0012 0 0.0002 0 0.0001 0.0001 0.0001 0 0.0001 0 0.0006 0 0.0003 0.0001
Endoplasmic Reticulum 0.0064 0.0028 0.0046 0.0038 0.0022 0.006 0.007 0.0023 0.0061 0.0076 0.0019 0.0022 0.0052 0.0013 0.0028 0.0038 0.0066 0.0018
Endosome 0.0335 0.0076 0.0262 0.0209 0.0659 0.0123 0.0376 0.003 0.022 0.0411 0.1032 0.0127 0.0349 0.0013 0.0136 0.0251 0.0498 0.0043
Golgi 0.0104 0.0004 0.0088 0.019 0.0419 0.0014 0.0125 0.0004 0.0075 0.007 0.0184 0.0062 0.0068 0.0001 0.0037 0.0007 0.0162 0.0022
Lipid Particles 0.0161 0.0007 0.0019 0.0072 0.0467 0.005 0.0152 0.0001 0.0049 0.0025 0.009 0.0013 0.0074 0 0.0017 0.0006 0.0114 0.0042
Mitochondria 0.0059 0.0003 0.0034 0.0466 0.0848 0.0011 0.0169 0.0001 0.0128 0.0139 0.0293 0.0089 0.0033 0.0001 0.0044 0.0018 0.0093 0.0063
None 0.1704 0.0818 0.0915 0.1153 0.053 0.1069 0.1565 0.0745 0.0926 0.0922 0.0246 0.0887 0.0842 0.0341 0.0396 0.0168 0.0389 0.0359
Nuclear Periphery 0.008 0.0022 0.0045 0.0013 0.0022 0.0022 0.0065 0.0005 0.0021 0.0035 0.0017 0.001 0.0119 0.0006 0.0024 0.0106 0.0178 0.0012
Nucleolus 0.0018 0.0001 0.0004 0.0001 0.0025 0.0001 0.0006 0 0.0003 0.0003 0.0008 0.0001 0.0013 0 0.0002 0.0002 0.0009 0.0001
Nucleus 0.0085 0.0043 0.0047 0.0024 0.004 0.0049 0.004 0.0017 0.004 0.0038 0.003 0.0038 0.0171 0.0025 0.0043 0.0087 0.0105 0.0085
Peroxisomes 0.0159 0.0003 0.0014 0.0052 0.0131 0.0093 0.0114 0.0003 0.0035 0.0027 0.0116 0.0007 0.0041 0.0001 0.0022 0.0003 0.0045 0.0074
Punctate Nuclear 0.0122 0.002 0.005 0.0011 0.0049 0.0021 0.0082 0.0003 0.0029 0.0031 0.0019 0.0011 0.0063 0.0006 0.003 0.0015 0.0182 0.0018
Vacuole 0.0034 0.0021 0.0028 0.0016 0.0082 0.0016 0.0035 0.001 0.0031 0.004 0.0047 0.0034 0.0046 0.0005 0.0018 0.004 0.0045 0.0007
Vacuole Periphery 0.0009 0.0004 0.0006 0.0006 0.0034 0.0003 0.0007 0.0001 0.001 0.0008 0.0016 0.0015 0.0018 0.0001 0.0004 0.0019 0.0025 0.0002

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 19.4786 11.7792 11.0208 13.794 18.1983 12.5968 14.499 11.7081 9.7602 20.8639
Translational Efficiency 1.1468 1.0452 0.8089 0.7978 0.8518 1.4453 0.7216 0.6107 0.9228 0.7068

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
968 1543 282 1597 2241 1937 204 162 3209 3480 486 1759

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 596.98 852.52 1103.37 1088.73 780.34 830.60 1079.90 1102.00 725.03 840.32 1093.52 1089.95
Standard Deviation 66.72 112.40 165.73 157.58 94.82 111.83 122.04 154.52 121.26 112.61 149.41 157.35
Intensity Change Log 2 — 0.514051 0.886162 0.866892 — 0.090051 0.468723 0.497949 — 0.289274 0.664627 0.669548

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000107 0.000163 0.000583 0.000660 0.000119 0.000253 0.000200 0.000328 0.000116 0.000213 0.000422 0.000630
Bud Neck 0.013675 0.005076 0.001345 0.001714 0.005518 0.012237 0.001687 0.001668 0.007979 0.009062 0.001488 0.001710
Bud Site 0.005955 0.010958 0.008998 0.016193 0.006362 0.020224 0.003978 0.011968 0.006239 0.016115 0.006891 0.015804
Cell Periphery 0.000251 0.000053 0.000171 0.000098 0.000068 0.000058 0.000152 0.000093 0.000123 0.000056 0.000163 0.000097
Cytoplasm 0.556741* 0.733347* 0.635799* 0.739823* 0.678845* 0.661387* 0.430425* 0.508654* 0.642013* 0.693293* 0.549592* 0.718533*
Cytoplasmic Foci 0.239168* 0.108792 0.037083 0.017896 0.154672 0.141649 0.041347 0.039677 0.180160* 0.127081 0.038873 0.019902
Eisosomes 0.000252 0.000021 0.000034 0.000013 0.000043 0.000036 0.000083 0.000031 0.000106 0.000029 0.000054 0.000015
Endoplasmic Reticulum 0.002021 0.000562 0.002364 0.000592 0.000606 0.000322 0.002354 0.000481 0.001033 0.000429 0.002360 0.000582
Endosome 0.006698 0.009062 0.004955 0.001665 0.007808 0.014819 0.011928 0.053388 0.007474 0.012267 0.007882 0.006429
Golgi 0.003238 0.001516 0.000291 0.000899 0.003430 0.006128 0.000127 0.015395 0.003372 0.004083 0.000222 0.002234
Lipid Particles 0.008915 0.000833 0.001322 0.000526 0.001722 0.001094 0.002630 0.000683 0.003892 0.000978 0.001871 0.000540
Mitochondria 0.009882 0.001318 0.000134 0.001518 0.001540 0.003294 0.000153 0.001900 0.004057 0.002417 0.000142 0.001553
Mitotic Spindle 0.000105 0.003750 0.001728 0.019175 0.001141 0.010171 0.000376 0.007574 0.000829 0.007324 0.001160 0.018106
None 0.007917 0.002779 0.002579 0.008344 0.003010 0.001941 0.002572 0.006789 0.004490 0.002312 0.002576 0.008201
Nuclear Periphery 0.000341 0.000116 0.000553 0.000406 0.000107 0.000077 0.001049 0.000462 0.000178 0.000094 0.000761 0.000411
Nuclear Periphery Foci 0.000246 0.000815 0.011423 0.002419 0.000391 0.000182 0.005093 0.001323 0.000347 0.000463 0.008766 0.002319
Nucleolus 0.001360 0.000514 0.000136 0.000136 0.000369 0.000595 0.000854 0.000159 0.000668 0.000559 0.000437 0.000138
Nucleus 0.105532 0.040971 0.127296 0.101531 0.058817 0.027352 0.119195 0.043276 0.072909 0.033391 0.123896 0.096166
Peroxisomes 0.002355 0.001937 0.000239 0.001571 0.001299 0.004197 0.000365 0.001281 0.001618 0.003195 0.000292 0.001544
Vacuole 0.033401 0.076067 0.162753 0.082941 0.072801 0.091676 0.375051* 0.299014* 0.060916 0.084755 0.251866* 0.102840
Vacuole Periphery 0.001839 0.001350 0.000215 0.001879 0.001330 0.002308 0.000382 0.005855 0.001484 0.001883 0.000285 0.002246

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -2.46 -6.74 -2.79 -2.39 0.90 -2.67 -5.61 -1.76 -0.78 -0.44 -3.28 -7.74 -2.88 -2.15 0.06
Bud Neck 5.15 7.41 7.45 5.44 0.13 -7.65 5.98 10.16 12.55 1.28 -1.41 10.36 11.27 13.46 0.97
Bud Site -3.93 -3.23 -2.52 1.25 0.61 -10.60 2.09 -1.67 2.32 -2.04 -11.02 -2.43 -3.16 5.37 -1.34
Cell Periphery 13.74 3.15 11.30 -4.07 8.37 2.72 -6.94 -1.37 -1.77 1.29 12.41 -5.23 4.87 -4.78 8.57
Cytoplasm -17.14 0.25 -3.28 14.84 -2.27 2.19 15.80 11.33 10.51 -1.62 -7.93 12.60 8.59 14.70 -7.12
Cytoplasmic Foci 18.84 30.17 37.52 24.29 5.33 2.61 28.55 27.74 24.29 0.99 13.07 38.44 50.62 39.73 6.47
Eisosomes 11.44 10.44 11.50 0.45 7.14 2.03 -6.88 3.22 0.83 8.61 11.43 6.59 12.95 4.14 12.17
Endoplasmic Reticulum 3.76 -1.55 3.48 -1.41 6.57 5.08 -7.83 -0.49 -3.78 7.55 4.79 -7.58 2.69 -4.83 9.82
Endosome -2.81 2.78 11.88 12.43 9.12 -7.15 -0.45 -0.51 0.80 -0.42 -7.24 2.09 9.74 13.65 6.66
Golgi 5.05 9.39 8.92 5.89 0.24 -4.92 15.70 0.33 1.55 -1.24 -1.94 17.97 10.24 9.74 -1.23
Lipid Particles 13.39 11.78 13.19 -2.01 5.68 4.35 -3.36 3.98 -0.27 5.79 13.66 7.83 13.97 -0.38 8.63
Mitochondria 4.92 5.80 5.53 1.31 -1.20 -2.69 4.51 2.69 4.49 -0.96 2.49 7.04 5.86 4.05 -1.29
Mitotic Spindle -3.33 -4.46 -7.57 -4.58 -6.43 -5.08 0.21 -3.07 -1.64 -3.09 -5.80 -1.66 -7.65 -3.19 -7.31
None 3.06 3.29 1.06 -4.93 -5.53 2.63 1.24 -0.80 -1.61 -1.27 3.74 3.51 -1.81 -6.22 -5.88
Nuclear Periphery 4.20 -6.00 -8.88 -20.96 -1.06 3.08 -11.32 -9.40 -9.80 4.17 4.62 -13.99 -19.26 -23.68 2.76
Nuclear Periphery Foci -6.86 -13.08 -11.19 -6.77 10.92 3.99 -7.00 -5.68 -6.87 5.17 -2.25 -14.36 -10.99 -10.20 11.43
Nucleolus 1.90 2.91 2.85 2.73 -1.76 -2.99 -1.04 7.76 6.41 2.29 0.74 2.34 3.95 5.68 1.99
Nucleus 13.98 -3.19 -6.25 -23.51 -0.32 15.43 -6.98 -1.36 -4.81 4.52 19.10 -8.86 -15.43 -27.04 0.29
Peroxisomes 1.61 12.11 7.15 4.74 -1.38 -5.26 9.18 4.93 6.79 -0.58 -4.74 14.61 5.68 7.40 -1.35
Vacuole -13.30 -15.04 -28.28 -19.38 1.37 -5.35 -17.30 -14.24 -13.44 0.19 -9.82 -20.40 -26.92 -22.35 6.19
Vacuole Periphery 2.01 7.45 4.42 3.07 -1.07 -4.46 11.31 0.30 1.40 -0.97 -2.31 13.87 4.11 5.23 -1.26
Standard name
Human Ortholog
Description Central kinetochore associated protein; mediates mitotic progression; interacts with several central kinetochore proteins and centromeric histone Cse4p; role in resistance to oxidative stress; similar to Slk19p; YBP2 has a paralog, YBP1, that arose from the whole genome duplication
Localization
Cell Percentages cytoplasm (82%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

Ybp2

Ybp2


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Ybp2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available