Standard name | |
---|---|
Human Ortholog | |
Description | Mitochondrial protein kinase; negatively regulates activity of the pyruvate dehydrogenase complex by phosphorylating the ser-133 residue of the Pda1p subunit; acts in concert with kinase Pkp1p and phosphatases Ptc5p and Ptc6p; relocalizes from mitochondrion to cytoplasm upon DNA replication stress |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 1 | – | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 4 | 5 | 3 | 1 | 4 | 0 | 1 | – | 4 | 2 | 3 | 7 |
Bud | 7 | – | 15 | 31 | 16 | 21 | 37 | 33 | 26 | 32 | 24 | 31 | 5 | 15 | 11 | 6 | 23 | 3 | 2 | – | 3 | 1 | 4 | 3 |
Bud Neck | 0 | – | 0 | 1 | 1 | 0 | 2 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 1 | 1 | 1 | 0 | – | 0 | 1 | 3 | 0 |
Bud Site | 2 | – | 1 | 4 | 5 | 6 | 13 | 11 | 10 | 17 | 12 | 11 | 0 | 0 | 0 | 3 | 5 | 4 | – | – | – | – | – | – |
Cell Periphery | 0 | – | 4 | 4 | 3 | 1 | 1 | 2 | 4 | 2 | 2 | 1 | 1 | 4 | 3 | 8 | 10 | 3 | 0 | – | 0 | 0 | 0 | 0 |
Cytoplasm | 5 | – | 4 | 7 | 13 | 10 | 12 | 17 | 18 | 8 | 6 | 8 | 12 | 25 | 40 | 13 | 17 | 17 | 1 | – | 0 | 0 | 6 | 2 |
Endoplasmic Reticulum | 1 | – | 3 | 1 | 0 | 0 | 1 | 0 | 2 | 0 | 1 | 0 | 1 | 0 | 2 | 3 | 7 | 7 | 0 | – | 0 | 1 | 1 | 2 |
Endosome | 3 | – | 47 | 12 | 21 | 5 | 1 | 9 | 2 | 1 | 0 | 0 | 42 | 90 | 63 | 56 | 51 | 50 | 14 | – | 28 | 15 | 40 | 30 |
Golgi | 0 | – | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 6 | 2 | 6 | 5 | 5 | 8 | 7 | – | 14 | 4 | 20 | 10 |
Mitochondria | 136 | – | 221 | 199 | 216 | 298 | 413 | 502 | 376 | 443 | 367 | 428 | 92 | 59 | 63 | 27 | 19 | 18 | 105 | – | 195 | 107 | 176 | 139 |
Nucleus | 0 | – | 0 | 4 | 3 | 5 | 15 | 23 | 12 | 13 | 7 | 8 | 0 | 2 | 1 | 5 | 3 | 0 | 0 | – | 0 | 0 | 2 | 2 |
Nuclear Periphery | 1 | – | 3 | 2 | 1 | 5 | 6 | 3 | 7 | 7 | 4 | 11 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | – | 1 | 1 | 2 | 2 |
Nucleolus | 9 | – | 4 | 1 | 4 | 12 | 14 | 16 | 12 | 11 | 8 | 7 | 1 | 1 | 1 | 11 | 10 | 5 | 1 | – | 0 | 0 | 1 | 2 |
Peroxisomes | 9 | – | 63 | 31 | 35 | 7 | 6 | 21 | 5 | 6 | 2 | 6 | 43 | 48 | 49 | 40 | 26 | 19 | 13 | – | 38 | 8 | 18 | 18 |
SpindlePole | 12 | – | 27 | 30 | 31 | 19 | 23 | 58 | 31 | 25 | 13 | 24 | 33 | 92 | 59 | 39 | 45 | 31 | 6 | – | 11 | 5 | 17 | 26 |
Vac/Vac Membrane | 8 | – | 24 | 8 | 18 | 19 | 14 | 16 | 12 | 9 | 12 | 17 | 22 | 34 | 24 | 28 | 39 | 30 | 0 | – | 0 | 12 | 26 | 24 |
Unique Cell Count | 158 | 294 | 246 | 275 | 327 | 445 | 573 | 415 | 462 | 385 | 452 | 184 | 262 | 253 | 166 | 191 | 143 | 159 | 304 | 166 | 334 | 277 | ||
Labelled Cell Count | 194 | 417 | 335 | 367 | 409 | 558 | 711 | 517 | 575 | 458 | 553 | 262 | 378 | 326 | 246 | 265 | 196 | 159 | 304 | 166 | 334 | 277 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 4.7 | – | 5.6 | 5.1 | 4.7 | 3.8 | 3.6 | 3.6 | 3.6 | 3.3 | 3.1 | 3.1 | 6.5 | 6.3 | 6.2 | 7.5 | 7.3 | 7.7 | 6.4 | 6.6 | 7.0 |
Std Deviation (1e-4) | 0.7 | – | 1.3 | 1.5 | 1.4 | 1.2 | 1.3 | 1.4 | 1.2 | 0.9 | 1.0 | 0.9 | 1.2 | 1.1 | 1.2 | 1.3 | 1.4 | 1.5 | 1.1 | 1.2 | 1.6 |
Intensity Change (Log2) | – | – | – | -0.14 | -0.24 | -0.58 | -0.65 | -0.63 | -0.66 | -0.76 | -0.83 | -0.83 | 0.2 | 0.17 | 0.14 | 0.43 | 0.38 | 0.46 | 0.19 | 0.23 | 0.31 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 3.1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | -1.3 | 0.3 | -0.4 | -0.7 | 2.8 | -1.5 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2.5 | 0 |
Cytoplasm | 1.2 | 2.4 | 1.4 | 1.2 | 1.5 | 2.3 | 0 | 0 | 0 | 3.1 | 4.3 | 6.2 | 3.5 | 4.0 | 4.8 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | -4.1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1.9 | 5.0 | 2.6 | 4.4 | 2.9 | 4.5 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 1.6 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | -5.6 | -12.4 | -11.7 | -12.2 | -14.0 | -12.3 |
Nucleus | 0 | 0 | 0 | 3.2 | 3.5 | 0 | 2.9 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3.1 | 2.5 | 0 |
Peroxisomes | -2.7 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0.5 | -0.9 | -0.6 | 0.7 | -2.2 | -2.0 |
SpindlePole | 1.1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2.8 | 7.4 | 4.5 | 4.2 | 4.4 | 3.6 |
Vacuole | -2.4 | -0.7 | -1.2 | -3.0 | -3.6 | -3.2 | -4.1 | -2.9 | -2.6 | 1.4 | 1.9 | 0.5 | 2.8 | 3.9 | 3.8 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 4.5869 | 5.0521 | 4.4529 | 4.1638 | 3.616 | 4.4832 | 2.6496 | 3.4029 | 3.0924 | 2.8458 | 2.2285 | 3.3325 | 2.2499 | 3.2734 | 2.7998 | 2.4119 | 2.242 | 3.0001 |
Actin | 0.0251 | 0.0104 | 0.0044 | 0.002 | 0.0011 | 0.0074 | 0.0245 | 0.0139 | 0.0078 | 0.0068 | 0.0015 | 0.0027 | 0.0159 | 0.0081 | 0.0158 | 0.0026 | 0.0353 | 0.0059 |
Bud | 0.0005 | 0.0005 | 0.0004 | 0.0017 | 0.0002 | 0.0003 | 0.0008 | 0.0031 | 0.0007 | 0.0009 | 0.0003 | 0.0002 | 0.0003 | 0.0007 | 0.0005 | 0.0001 | 0.0008 | 0.0002 |
Bud Neck | 0.0027 | 0.0004 | 0.0003 | 0.0003 | 0.0002 | 0.0004 | 0.0097 | 0.0008 | 0.0059 | 0.0015 | 0.0002 | 0.0002 | 0.0004 | 0.0003 | 0.0003 | 0.0001 | 0.0011 | 0.0002 |
Bud Periphery | 0.0015 | 0.0008 | 0.0009 | 0.0058 | 0.0005 | 0.0006 | 0.0013 | 0.0034 | 0.0016 | 0.001 | 0.0012 | 0.0003 | 0.0007 | 0.0009 | 0.0005 | 0.0002 | 0.0019 | 0.0004 |
Bud Site | 0.009 | 0.0055 | 0.0048 | 0.0023 | 0.0003 | 0.0004 | 0.0069 | 0.0242 | 0.0028 | 0.001 | 0.0005 | 0.0002 | 0.0007 | 0.0029 | 0.0005 | 0.0002 | 0.0005 | 0.0002 |
Cell Periphery | 0.0027 | 0.0004 | 0.0018 | 0.0003 | 0.0001 | 0.0002 | 0.0005 | 0.001 | 0.0007 | 0.0001 | 0.0001 | 0.0001 | 0.0004 | 0.0002 | 0.0002 | 0.0001 | 0.0002 | 0.0001 |
Cytoplasm | 0.0006 | 0.0007 | 0.0004 | 0.0056 | 0.0098 | 0.0003 | 0.0026 | 0.0026 | 0.0025 | 0.0017 | 0.0003 | 0.0012 | 0.0015 | 0.0002 | 0.0046 | 0.0001 | 0.0003 | 0.0001 |
Cytoplasmic Foci | 0.0197 | 0.0173 | 0.0114 | 0.0223 | 0.019 | 0.007 | 0.0322 | 0.0223 | 0.0165 | 0.0506 | 0.048 | 0.0059 | 0.0201 | 0.0131 | 0.0086 | 0.0093 | 0.0064 | 0.0044 |
Eisosomes | 0.0011 | 0.0002 | 0.001 | 0.0003 | 0.0001 | 0.0002 | 0.0004 | 0.0003 | 0.0002 | 0.0001 | 0.0001 | 0.0001 | 0.0002 | 0.0002 | 0.0002 | 0.0002 | 0.0005 | 0.0002 |
Endoplasmic Reticulum | 0.0006 | 0.0011 | 0.0001 | 0.0016 | 0.0002 | 0.0002 | 0.0041 | 0.0007 | 0.0012 | 0.0005 | 0.0002 | 0.0002 | 0.0019 | 0.0001 | 0.0007 | 0.0001 | 0.0009 | 0.0005 |
Endosome | 0.027 | 0.0544 | 0.0207 | 0.0547 | 0.0407 | 0.0219 | 0.0961 | 0.073 | 0.0589 | 0.0641 | 0.0602 | 0.04 | 0.0533 | 0.0286 | 0.0245 | 0.035 | 0.0172 | 0.0184 |
Golgi | 0.0397 | 0.0471 | 0.029 | 0.0246 | 0.0294 | 0.0349 | 0.065 | 0.0783 | 0.0583 | 0.0285 | 0.0711 | 0.0602 | 0.0469 | 0.0404 | 0.0425 | 0.0644 | 0.0454 | 0.0517 |
Lipid Particles | 0.0578 | 0.015 | 0.0094 | 0.0074 | 0.0434 | 0.0137 | 0.0368 | 0.0266 | 0.0176 | 0.0324 | 0.0169 | 0.0122 | 0.0445 | 0.0112 | 0.0077 | 0.0252 | 0.0049 | 0.0146 |
Mitochondria | 0.7346 | 0.795 | 0.8655 | 0.7745 | 0.8339 | 0.8844 | 0.6344 | 0.6967 | 0.7744 | 0.7595 | 0.7579 | 0.8519 | 0.7385 | 0.8484 | 0.8509 | 0.8444 | 0.8617 | 0.8804 |
None | 0.0085 | 0.0001 | 0.0002 | 0.0321 | 0.0002 | 0.0001 | 0.0012 | 0.0002 | 0.0005 | 0.0004 | 0.0001 | 0.0001 | 0.0035 | 0.0001 | 0.0024 | 0.0001 | 0.0006 | 0.0001 |
Nuclear Periphery | 0.0014 | 0.0009 | 0 | 0.0016 | 0.001 | 0.0001 | 0.0219 | 0.0004 | 0.0037 | 0.004 | 0.0001 | 0.0005 | 0.0091 | 0.0001 | 0.0012 | 0.0001 | 0.0025 | 0.0004 |
Nucleolus | 0.0017 | 0.0003 | 0.0004 | 0.0011 | 0.0003 | 0.0001 | 0.001 | 0.003 | 0.0016 | 0.0026 | 0.0002 | 0.0033 | 0.0011 | 0.0003 | 0.0004 | 0.0002 | 0.0003 | 0.0001 |
Nucleus | 0.0005 | 0.0002 | 0.0001 | 0.0009 | 0.0002 | 0.0001 | 0.0046 | 0.0007 | 0.0038 | 0.0017 | 0.0001 | 0.0006 | 0.0009 | 0.0002 | 0.0003 | 0.0001 | 0.0004 | 0.0001 |
Peroxisomes | 0.05 | 0.0333 | 0.0421 | 0.0294 | 0.0134 | 0.0242 | 0.0276 | 0.0141 | 0.0179 | 0.0257 | 0.0346 | 0.0123 | 0.0229 | 0.0344 | 0.0332 | 0.0142 | 0.0071 | 0.019 |
Punctate Nuclear | 0.002 | 0.0001 | 0.0001 | 0.0017 | 0.0003 | 0.0001 | 0.0027 | 0.0003 | 0.0004 | 0.0044 | 0.0004 | 0.0002 | 0.0097 | 0.0001 | 0.0008 | 0.0002 | 0.0014 | 0.0001 |
Vacuole | 0.0069 | 0.0082 | 0.0052 | 0.0141 | 0.0025 | 0.0013 | 0.0148 | 0.0197 | 0.0122 | 0.0077 | 0.0027 | 0.0025 | 0.0123 | 0.004 | 0.0016 | 0.0012 | 0.006 | 0.0008 |
Vacuole Periphery | 0.0065 | 0.008 | 0.0019 | 0.0158 | 0.0032 | 0.0022 | 0.0109 | 0.0147 | 0.0107 | 0.0047 | 0.0034 | 0.0052 | 0.0154 | 0.0058 | 0.0026 | 0.0018 | 0.0048 | 0.0024 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 5.9805 | 2.0048 | 2.4795 | 6.7162 | 3.3924 | 7.4715 | 9.2908 | 9.577 | 7.7367 | 9.9361 |
Translational Efficiency | 0.3507 | 1.3371 | 0.9872 | 0.442 | 0.7115 | 0.5659 | 0.4586 | 0.3359 | 0.6018 | 0.4164 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
132 | 891 | 1112 | 117 | 1928 | 1390 | 177 | 99 | 2060 | 2281 | 1289 | 216 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 761.27 | 863.93 | 886.83 | 901.94 | 775.66 | 865.35 | 980.76 | 957.09 | 774.74 | 864.80 | 899.73 | 927.22 |
Standard Deviation | 109.20 | 134.76 | 116.12 | 107.43 | 96.78 | 119.38 | 100.42 | 109.31 | 97.69 | 125.62 | 118.59 | 111.73 |
Intensity Change Log 2 | — | 0.182506 | 0.220249 | 0.244623 | — | 0.157859 | 0.338476 | 0.303230 | — | 0.170120 | 0.281126 | 0.274499 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.006635 | 0.005544 | 0.002754 | 0.018985 | 0.000742 | 0.002399 | 0.003390 | 0.031366 | 0.001120 | 0.003628 | 0.002841 | 0.024660 |
Bud Neck | 0.005288 | 0.005228 | 0.027104 | 0.017695 | 0.004616 | 0.005245 | 0.028595 | 0.027079 | 0.004659 | 0.005239 | 0.027309 | 0.021996 |
Bud Site | 0.024016 | 0.021601 | 0.034274 | 0.079924 | 0.006399 | 0.015888 | 0.033671 | 0.092788 | 0.007528 | 0.018120 | 0.034191 | 0.085820 |
Cell Periphery | 0.008636 | 0.000444 | 0.000200 | 0.001268 | 0.000550 | 0.000307 | 0.000320 | 0.000239 | 0.001068 | 0.000361 | 0.000216 | 0.000796 |
Cytoplasm | 0.005574 | 0.005778 | 0.065621 | 0.064121 | 0.004299 | 0.004518 | 0.156954* | 0.056997 | 0.004381 | 0.005010 | 0.078162 | 0.060856 |
Cytoplasmic Foci | 0.029670 | 0.061043 | 0.145127 | 0.152123 | 0.041866 | 0.061660 | 0.146868 | 0.208925* | 0.041085 | 0.061419 | 0.145366 | 0.178157 |
Eisosomes | 0.000178 | 0.000115 | 0.000121 | 0.000241 | 0.000073 | 0.000077 | 0.000217 | 0.000266 | 0.000079 | 0.000092 | 0.000134 | 0.000253 |
Endoplasmic Reticulum | 0.001500 | 0.000422 | 0.005631 | 0.007387 | 0.000739 | 0.000405 | 0.007737 | 0.004021 | 0.000787 | 0.000412 | 0.005920 | 0.005844 |
Endosome | 0.044506 | 0.107232 | 0.103334 | 0.071116 | 0.087809 | 0.136352 | 0.058374 | 0.054631 | 0.085034 | 0.124977 | 0.097160 | 0.063561 |
Golgi | 0.068733 | 0.104026 | 0.041818 | 0.026412 | 0.107990 | 0.125852* | 0.006059 | 0.028395 | 0.105475 | 0.117326* | 0.036908 | 0.027321 |
Lipid Particles | 0.004511 | 0.003709 | 0.012366 | 0.015595 | 0.006559 | 0.003427 | 0.014799 | 0.006988 | 0.006428 | 0.003537 | 0.012700 | 0.011650 |
Mitochondria | 0.745056* | 0.529368* | 0.084581 | 0.021749 | 0.657327* | 0.523337* | 0.013017 | 0.007836 | 0.662948* | 0.525693* | 0.074754 | 0.015372 |
Mitotic Spindle | 0.003441 | 0.020607 | 0.179084* | 0.122997* | 0.008484 | 0.028264 | 0.056033 | 0.087333 | 0.008161 | 0.025273 | 0.162187* | 0.106651* |
None | 0.001190 | 0.000215 | 0.002025 | 0.000330 | 0.000732 | 0.000581 | 0.002171 | 0.000423 | 0.000762 | 0.000438 | 0.002045 | 0.000373 |
Nuclear Periphery | 0.000340 | 0.000062 | 0.004029 | 0.000816 | 0.000202 | 0.000162 | 0.002206 | 0.000421 | 0.000210 | 0.000123 | 0.003779 | 0.000635 |
Nuclear Periphery Foci | 0.001766 | 0.000395 | 0.003609 | 0.005140 | 0.000404 | 0.000401 | 0.004396 | 0.001397 | 0.000491 | 0.000399 | 0.003717 | 0.003424 |
Nucleolus | 0.006882 | 0.000302 | 0.011926 | 0.002568 | 0.000740 | 0.000838 | 0.006694 | 0.001832 | 0.001134 | 0.000628 | 0.011207 | 0.002231 |
Nucleus | 0.006498 | 0.000402 | 0.062563 | 0.030035 | 0.001235 | 0.000896 | 0.197463* | 0.020672 | 0.001572 | 0.000703 | 0.081087 | 0.025744 |
Peroxisomes | 0.022274 | 0.113846* | 0.055767 | 0.244897* | 0.021307 | 0.050091 | 0.097441 | 0.231609* | 0.021369 | 0.074995 | 0.061489 | 0.238807* |
Vacuole | 0.004409 | 0.011268 | 0.137873 | 0.113152 | 0.021892 | 0.021179 | 0.154459 | 0.134227 | 0.020771 | 0.017307 | 0.140151 | 0.122812 |
Vacuole Periphery | 0.008896 | 0.008392 | 0.020193 | 0.003448 | 0.026036 | 0.018121 | 0.009138 | 0.002553 | 0.024938 | 0.014321 | 0.018675 | 0.003038 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | 0.31 | 1.10 | -1.58 | -2.03 | -2.54 | -3.82 | -5.49 | -3.79 | -3.54 | -3.38 | -5.26 | -5.28 | -4.67 | -4.08 | -4.24 |
Bud Neck | 0.02 | -7.38 | -3.26 | -3.99 | 2.33 | -0.66 | -3.83 | -4.28 | -4.16 | -0.01 | -0.70 | -12.56 | -5.85 | -5.72 | 1.44 |
Bud Site | 0.26 | -1.19 | -3.56 | -4.60 | -3.50 | -5.87 | -5.43 | -6.59 | -5.80 | -4.33 | -6.84 | -13.11 | -8.82 | -7.47 | -5.52 |
Cell Periphery | 1.32 | 1.36 | 1.24 | -1.19 | -1.79 | 1.39 | 1.34 | 1.87 | 1.92 | 2.09 | 1.64 | 1.98 | 0.95 | -1.11 | -1.75 |
Cytoplasm | -0.05 | -12.21 | -5.11 | -5.23 | -0.32 | -0.58 | -9.61 | -4.85 | -4.81 | 4.13 | -0.88 | -18.05 | -7.22 | -7.13 | 0.96 |
Cytoplasmic Foci | -5.41 | -16.79 | -8.33 | -6.08 | 0.33 | -6.53 | -8.52 | -10.07 | -8.68 | -3.21 | -7.90 | -22.05 | -12.49 | -10.31 | -2.23 |
Eisosomes | 1.27 | 1.02 | -1.14 | -4.60 | -4.19 | -0.75 | -9.24 | -6.70 | -6.57 | -1.11 | -2.07 | -9.11 | -8.58 | -8.10 | -5.50 |
Endoplasmic Reticulum | 2.10 | -6.94 | -3.38 | -4.05 | -0.97 | 2.41 | -7.54 | -4.20 | -4.56 | 3.06 | 2.86 | -13.16 | -5.17 | -5.52 | 0.04 |
Endosome | -6.74 | -6.01 | -0.95 | 4.69 | 3.90 | -8.42 | 5.52 | 4.92 | 10.21 | 0.04 | -8.47 | -1.09 | 4.63 | 10.18 | 5.31 |
Golgi | -2.94 | 2.81 | 4.05 | 11.07 | 2.93 | -2.96 | 26.59 | 13.13 | 14.65 | -3.18 | -2.35 | 17.07 | 15.91 | 18.42 | 2.75 |
Lipid Particles | 0.38 | -2.93 | -2.51 | -3.40 | -0.65 | 2.80 | -4.70 | -0.57 | -2.96 | 3.86 | 2.96 | -4.92 | -2.46 | -4.16 | 0.71 |
Mitochondria | 6.60 | 22.10 | 23.76 | 35.17* | 7.29 | 10.79 | 81.70* | 78.89* | 48.99* | 0.84 | 12.83 | 71.69* | 77.95* | 57.92* | 9.75 |
Mitotic Spindle | -6.62 | -21.21* | -5.30 | -4.50 | 2.04 | -7.62 | -3.92 | -4.42 | -3.22 | -1.60 | -8.79 | -20.66 | -6.67 | -5.44 | 3.07 |
None | 1.26 | -1.02 | 1.01 | -1.81 | 4.08 | 0.28 | -2.26 | 0.71 | 0.47 | 3.48 | 0.84 | -2.78 | 0.97 | 0.10 | 4.54 |
Nuclear Periphery | 1.47 | -4.64 | -2.69 | -4.37 | 3.54 | 0.66 | -7.73 | -2.09 | -2.30 | 6.34 | 1.99 | -5.38 | -4.19 | -4.75 | 4.34 |
Nuclear Periphery Foci | 1.48 | -2.62 | -3.07 | -4.75 | -1.62 | 0.01 | -6.40 | -3.83 | -3.84 | 3.80 | 0.61 | -9.52 | -5.34 | -5.50 | -0.01 |
Nucleolus | 1.64 | -1.25 | 0.95 | -2.59 | 6.24 | -0.37 | -4.42 | -2.58 | -2.19 | 2.86 | 1.43 | -10.37 | -2.21 | -3.13 | 7.80 |
Nucleus | 1.94 | -11.41 | -3.86 | -5.16 | 3.97 | 0.66 | -10.52 | -3.11 | -3.15 | 8.28 | 1.98 | -17.97 | -5.68 | -5.85 | 7.57 |
Peroxisomes | -9.50 | -4.35 | -7.21 | -3.30 | -6.06 | -7.86 | -4.79 | -8.07 | -6.74 | -4.89 | -13.92 | -10.00 | -10.93 | -7.46 | -8.60 |
Vacuole | -3.99 | -25.95 | -8.15 | -7.86 | -1.78 | 0.37 | -11.21 | -6.79 | -6.82 | 0.66 | 2.11 | -25.30 | -10.11 | -10.33 | -1.68 |
Vacuole Periphery | 0.22 | -3.72 | 2.56 | 3.83 | 8.67 | 4.42 | 7.14 | 16.37 | 10.91 | 1.85 | 7.23 | 4.97 | 15.96 | 10.52 | 10.21 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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