Standard name | |
---|---|
Human Ortholog | |
Description | Mitochondrial ribosomal protein of the large subunit; appears as two protein spots (YmL34 and YmL38) on two-dimensional SDS gels; protein abundance increases in response to DNA replication stress |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 4 | 5 | 25 | 17 | 6 | 1 | – | 8 | 8 | 5 | 11 | 4 | 7 | 2 | 2 | 0 | 0 | 0 | 10 | 7 | 11 | 2 | 12 | 6 |
Bud | 5 | 11 | 32 | 4 | 6 | 4 | – | 4 | 4 | 4 | 6 | 4 | 7 | 6 | 2 | 1 | 0 | 0 | 2 | 1 | 2 | 3 | 5 | 7 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 5 | 7 | 4 | 4 | 2 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 20 | 95 | 65 | 81 | 58 | 45 | – | 45 | 33 | 53 | 55 | 39 | 92 | 124 | 100 | 41 | 18 | 17 | 11 | 51 | 77 | 23 | 46 | 41 |
Cytoplasm | 62 | 113 | 87 | 85 | 118 | 158 | – | 254 | 161 | 171 | 208 | 189 | 107 | 116 | 115 | 40 | 45 | 16 | 29 | 102 | 39 | 33 | 69 | 74 |
Endoplasmic Reticulum | 22 | 34 | 19 | 56 | 87 | 68 | – | 117 | 40 | 47 | 42 | 67 | 35 | 29 | 33 | 0 | 3 | 1 | 39 | 48 | 62 | 29 | 70 | 60 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 2 | 1 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 2 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 5 | 4 |
Mitochondria | 0 | 1 | 1 | 2 | 0 | 2 | – | 2 | 6 | 9 | 13 | 7 | 1 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 1 | 0 | 2 | 1 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 3 | 1 |
Nuclear Periphery | 0 | 0 | 0 | 1 | 0 | 1 | – | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 1 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 3 | 0 | – | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vac/Vac Membrane | 0 | 0 | 1 | 0 | 2 | 1 | – | 3 | 0 | 3 | 0 | 0 | 0 | 1 | 3 | 2 | 5 | 1 | 0 | 0 | 1 | 1 | 6 | 3 |
Unique Cell Count | 89 | 193 | 170 | 182 | 223 | 236 | 386 | 215 | 247 | 279 | 276 | 187 | 197 | 200 | 68 | 53 | 24 | 102 | 221 | 206 | 103 | 237 | 215 | |
Labelled Cell Count | 113 | 259 | 230 | 246 | 280 | 280 | 433 | 253 | 295 | 337 | 312 | 249 | 278 | 257 | 85 | 73 | 35 | 102 | 221 | 206 | 103 | 237 | 215 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 24.4 | 30.2 | 19.9 | 26.0 | 28.5 | 29.2 | – | 27.9 | 27.3 | 25.9 | 24.6 | 24.7 | 35.6 | 40.8 | 42.6 | 58.8 | 56.0 | 57.2 | 34.6 | 34.8 | 34.9 |
Std Deviation (1e-4) | 7.4 | 5.8 | 10.3 | 6.1 | 6.4 | 6.5 | – | 6.4 | 6.6 | 8.2 | 6.5 | 7.9 | 10.9 | 12.9 | 14.2 | 29.9 | 31.5 | 26.6 | 18.3 | 18.8 | 17.3 |
Intensity Change (Log2) | – | – | – | 0.39 | 0.52 | 0.56 | – | 0.49 | 0.46 | 0.38 | 0.31 | 0.31 | 0.84 | 1.04 | 1.1 | 1.56 | 1.49 | 1.52 | 0.8 | 0.81 | 0.81 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | -1.6 | -4.4 | -5.8 | – | -5.8 | -3.8 | -4.9 | -4.1 | -5.5 | -3.6 | -5.0 | -5.1 | -3.3 | -3.0 | 0 |
Bud | -5.1 | -5.4 | -6.0 | – | -7.9 | -5.7 | -6.1 | -6.2 | -6.5 | -4.6 | -4.9 | -5.9 | -3.5 | -3.4 | 0 |
Bud Neck | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 1.2 | -2.6 | -4.3 | – | -7.2 | -5.1 | -3.7 | -4.3 | -5.8 | 2.1 | 4.7 | 2.3 | 3.1 | -0.6 | 3.0 |
Cytoplasm | -0.8 | 0.3 | 3.2 | – | 3.3 | 4.8 | 3.7 | 5.1 | 3.7 | 1.1 | 1.5 | 1.2 | 1.1 | 4.4 | 1.4 |
Endoplasmic Reticulum | 4.5 | 6.2 | 4.3 | – | 4.8 | 2.0 | 2.2 | 1.2 | 3.4 | 2.0 | 1.0 | 1.5 | -2.9 | -1.2 | 0 |
Endosome | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 0 | – | 0 | 0 | 0 | 2.4 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 24.1915 | 27.2059 | 24.6365 | 24.6791 | 21.883 | 26.2416 | 24.1319 | 27.9316 | 24.1575 | 27.1053 | 22.6325 | 25.8192 | 26.3112 | 27.6178 | 26.0298 | 21.5911 | 26.5773 | 27.1041 |
Actin | 0.046 | 0.0148 | 0.0374 | 0.0455 | 0.0652 | 0.0374 | 0.0338 | 0.0268 | 0.0534 | 0.014 | 0.0366 | 0.0296 | 0.0365 | 0.0203 | 0.0633 | 0.1057 | 0.0287 | 0.0583 |
Bud | 0.0075 | 0.0009 | 0.0156 | 0.0105 | 0.0028 | 0.0017 | 0.0026 | 0.002 | 0.0152 | 0.0048 | 0.0045 | 0.0009 | 0.0035 | 0.0013 | 0.0176 | 0.0046 | 0.0012 | 0.0011 |
Bud Neck | 0.0072 | 0.007 | 0.019 | 0.0157 | 0.0397 | 0.1173 | 0.0115 | 0.0061 | 0.0097 | 0.0291 | 0.0285 | 0.1324 | 0.0099 | 0.0063 | 0.0115 | 0.0105 | 0.0168 | 0.1399 |
Bud Periphery | 0.0213 | 0.0022 | 0.0518 | 0.0433 | 0.0088 | 0.0121 | 0.0098 | 0.0056 | 0.0601 | 0.0462 | 0.0274 | 0.0079 | 0.0134 | 0.0026 | 0.0654 | 0.0309 | 0.0119 | 0.0097 |
Bud Site | 0.0175 | 0.0988 | 0.0188 | 0.0087 | 0.0242 | 0.0014 | 0.012 | 0.0798 | 0.0284 | 0.0017 | 0.0023 | 0.0017 | 0.0146 | 0.1152 | 0.027 | 0.0087 | 0.0012 | 0.0011 |
Cell Periphery | 0.1493 | 0.2263 | 0.2445 | 0.238 | 0.1241 | 0.1547 | 0.1905 | 0.281 | 0.2992 | 0.3255 | 0.2575 | 0.1759 | 0.1788 | 0.2338 | 0.2547 | 0.2146 | 0.1822 | 0.1109 |
Cytoplasm | 0.4566 | 0.4727 | 0.3999 | 0.2363 | 0.4271 | 0.3823 | 0.3502 | 0.3813 | 0.3328 | 0.295 | 0.2958 | 0.2995 | 0.3884 | 0.4724 | 0.3692 | 0.2453 | 0.3352 | 0.3051 |
Cytoplasmic Foci | 0.0054 | 0.0015 | 0.0073 | 0.0038 | 0.0451 | 0.0103 | 0.0151 | 0.0024 | 0.0059 | 0.0006 | 0.0023 | 0.0027 | 0.0081 | 0.0018 | 0.0037 | 0.0063 | 0.0176 | 0.0053 |
Eisosomes | 0.0017 | 0.0014 | 0.001 | 0.001 | 0.0009 | 0.0015 | 0.0042 | 0.0018 | 0.0019 | 0.001 | 0.005 | 0.0017 | 0.0014 | 0.0014 | 0.002 | 0.0017 | 0.0025 | 0.0014 |
Endoplasmic Reticulum | 0.2042 | 0.1387 | 0.1344 | 0.2821 | 0.1858 | 0.2431 | 0.2688 | 0.1659 | 0.1258 | 0.238 | 0.2656 | 0.3056 | 0.2634 | 0.117 | 0.1456 | 0.2075 | 0.3376 | 0.3285 |
Endosome | 0.0105 | 0.0006 | 0.011 | 0.0165 | 0.0095 | 0.0028 | 0.0165 | 0.0013 | 0.0021 | 0.0004 | 0.0124 | 0.0016 | 0.0073 | 0.0008 | 0.0034 | 0.0147 | 0.0045 | 0.0077 |
Golgi | 0.01 | 0.0031 | 0.0081 | 0.0253 | 0.011 | 0.0105 | 0.0122 | 0.0049 | 0.0048 | 0.0014 | 0.0164 | 0.0059 | 0.0078 | 0.0043 | 0.0043 | 0.013 | 0.0106 | 0.0115 |
Lipid Particles | 0.0148 | 0.017 | 0.0142 | 0.0338 | 0.0238 | 0.0109 | 0.0259 | 0.0234 | 0.0172 | 0.0106 | 0.0179 | 0.0173 | 0.0151 | 0.0112 | 0.0132 | 0.0439 | 0.0165 | 0.0074 |
Mitochondria | 0.0129 | 0.0001 | 0.0134 | 0.0116 | 0.0087 | 0.002 | 0.0075 | 0.0005 | 0.0125 | 0.0001 | 0.0053 | 0.0033 | 0.0125 | 0.0002 | 0.0014 | 0.0562 | 0.0006 | 0.0007 |
None | 0.0036 | 0.0003 | 0.0006 | 0.0004 | 0.0014 | 0.0006 | 0.003 | 0.0002 | 0.0026 | 0.0002 | 0.0004 | 0.0003 | 0.009 | 0.0002 | 0.0015 | 0.0019 | 0.0209 | 0.0006 |
Nuclear Periphery | 0.0116 | 0.0063 | 0.0075 | 0.0065 | 0.0033 | 0.004 | 0.0111 | 0.0057 | 0.0066 | 0.0053 | 0.0051 | 0.0046 | 0.0089 | 0.0051 | 0.0066 | 0.0083 | 0.0043 | 0.0048 |
Nucleolus | 0.0007 | 0.0002 | 0.0004 | 0.0003 | 0.0002 | 0.0003 | 0.0009 | 0.0002 | 0.0011 | 0.0001 | 0.0002 | 0.0002 | 0.0003 | 0.0001 | 0.0001 | 0.0013 | 0.0002 | 0.0001 |
Nucleus | 0.0023 | 0.001 | 0.0019 | 0.0015 | 0.0011 | 0.0012 | 0.0029 | 0.0009 | 0.002 | 0.001 | 0.001 | 0.0013 | 0.0018 | 0.001 | 0.0015 | 0.002 | 0.0007 | 0.0012 |
Peroxisomes | 0.0013 | 0.0001 | 0.001 | 0.0005 | 0.0101 | 0.0003 | 0.002 | 0.0001 | 0.0051 | 0 | 0.0002 | 0.0002 | 0.0027 | 0.0001 | 0.0007 | 0.0063 | 0.0013 | 0.0003 |
Punctate Nuclear | 0.0006 | 0.0002 | 0.0004 | 0.0003 | 0.0019 | 0.0003 | 0.0023 | 0.0002 | 0.0004 | 0.0001 | 0.0002 | 0.0002 | 0.0035 | 0.0002 | 0.0006 | 0.0035 | 0.0024 | 0.0008 |
Vacuole | 0.0137 | 0.0069 | 0.0108 | 0.0168 | 0.005 | 0.0051 | 0.016 | 0.0096 | 0.0125 | 0.0243 | 0.0133 | 0.0067 | 0.012 | 0.0045 | 0.0065 | 0.0102 | 0.0026 | 0.0033 |
Vacuole Periphery | 0.0013 | 0.0002 | 0.0008 | 0.0016 | 0.0004 | 0.0004 | 0.0012 | 0.0002 | 0.0006 | 0.0007 | 0.0021 | 0.0004 | 0.0011 | 0.0001 | 0.0004 | 0.0029 | 0.0003 | 0.0004 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 14.5533 | 7.0962 | 10.5314 | 26.1497 | 22.2997 | 37.0242 | 26.3083 | 34.7056 | 42.1303 | 38.6863 |
Translational Efficiency | 2.6529 | 4.7676 | 3.4738 | 1.3144 | 1.5965 | 1.5426 | 2.1698 | 1.8704 | 1.4715 | 1.7445 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✔ |
Gene Expression | ✔ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
1485 | 911 | 263 | 1300 | 395 | 1563 | 1330 | 1497 | 1880 | 2474 | 1593 | 2797 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 1705.04 | 2061.95 | 3717.47 | 3940.60 | 2446.48 | 1915.90 | 2963.40 | 3740.99 | 1860.82 | 1969.68 | 3087.89 | 3833.77 |
Standard Deviation | 393.56 | 501.51 | 604.33 | 946.99 | 563.49 | 362.84 | 520.40 | 965.28 | 529.43 | 425.15 | 603.97 | 961.99 |
Intensity Change Log 2 | — | 0.274204 | 1.124516 | 1.208610 | — | -0.352685 | 0.276546 | 0.612713 | — | -0.061651 | 0.686396 | 0.887765 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.003661 | 0.007882 | 0.003914 | 0.007006 | 0.003601 | 0.006613 | 0.008059 | 0.006283 | 0.003648 | 0.007080 | 0.007374 | 0.006619 |
Bud Neck | 0.006837 | 0.011782 | 0.012772 | 0.008880 | 0.005472 | 0.009750 | 0.010755 | 0.007517 | 0.006550 | 0.010498 | 0.011088 | 0.008150 |
Bud Site | 0.086598 | 0.082567 | 0.015838 | 0.081072 | 0.035460 | 0.073547 | 0.042160 | 0.053235 | 0.075853 | 0.076868 | 0.037814 | 0.066174 |
Cell Periphery | 0.278213* | 0.441667* | 0.631549* | 0.527715* | 0.485550* | 0.451025* | 0.640584* | 0.625088* | 0.321776* | 0.447579* | 0.639092* | 0.579831* |
Cytoplasm | 0.149173* | 0.072442 | 0.002735 | 0.104810* | 0.008770 | 0.078354 | 0.044967 | 0.084303 | 0.119673* | 0.076177 | 0.037995 | 0.093834 |
Cytoplasmic Foci | 0.045747 | 0.013089 | 0.000818 | 0.005008 | 0.005739 | 0.013538 | 0.002015 | 0.002733 | 0.037341 | 0.013373 | 0.001817 | 0.003790 |
Eisosomes | 0.000520 | 0.001438 | 0.004182 | 0.001181 | 0.000795 | 0.001466 | 0.002861 | 0.001299 | 0.000578 | 0.001456 | 0.003079 | 0.001244 |
Endoplasmic Reticulum | 0.170808* | 0.153079* | 0.027822 | 0.008752 | 0.224003* | 0.186362* | 0.036619 | 0.015328 | 0.181985* | 0.174106* | 0.035166 | 0.012272 |
Endosome | 0.018862 | 0.010558 | 0.003689 | 0.002332 | 0.010103 | 0.009503 | 0.002855 | 0.003219 | 0.017022 | 0.009891 | 0.002992 | 0.002807 |
Golgi | 0.035395 | 0.017085 | 0.016612 | 0.006094 | 0.058172 | 0.010136 | 0.004143 | 0.005518 | 0.040181 | 0.012695 | 0.006202 | 0.005786 |
Lipid Particles | 0.129459* | 0.128154* | 0.103917* | 0.134459* | 0.075880 | 0.106192* | 0.111273* | 0.103628* | 0.118201* | 0.114279* | 0.110058* | 0.117958* |
Mitochondria | 0.002168 | 0.002131 | 0.000221 | 0.000649 | 0.002369 | 0.003357 | 0.000835 | 0.001291 | 0.002210 | 0.002906 | 0.000734 | 0.000992 |
Mitotic Spindle | 0.001622 | 0.002352 | 0.001110 | 0.002599 | 0.001886 | 0.001652 | 0.001492 | 0.001493 | 0.001678 | 0.001910 | 0.001429 | 0.002007 |
None | 0.001258 | 0.000341 | 0.000109 | 0.000303 | 0.000279 | 0.000273 | 0.000219 | 0.000276 | 0.001052 | 0.000298 | 0.000201 | 0.000288 |
Nuclear Periphery | 0.001286 | 0.002664 | 0.004809 | 0.002817 | 0.002834 | 0.002383 | 0.004361 | 0.003086 | 0.001611 | 0.002486 | 0.004435 | 0.002961 |
Nuclear Periphery Foci | 0.007819 | 0.004864 | 0.002968 | 0.002423 | 0.003645 | 0.002248 | 0.001891 | 0.002866 | 0.006942 | 0.003211 | 0.002069 | 0.002660 |
Nucleolus | 0.000146 | 0.000108 | 0.000059 | 0.000099 | 0.000103 | 0.000116 | 0.000098 | 0.000112 | 0.000137 | 0.000113 | 0.000091 | 0.000106 |
Nucleus | 0.001776 | 0.001639 | 0.001137 | 0.004131 | 0.002542 | 0.001408 | 0.002447 | 0.002671 | 0.001937 | 0.001493 | 0.002231 | 0.003349 |
Peroxisomes | 0.008859 | 0.008620 | 0.000119 | 0.000968 | 0.000588 | 0.013107 | 0.002909 | 0.001845 | 0.007122 | 0.011455 | 0.002449 | 0.001437 |
Vacuole | 0.033491 | 0.030519 | 0.154612 | 0.094217 | 0.036684 | 0.022084 | 0.076022 | 0.073102 | 0.034162 | 0.025190 | 0.088997 | 0.082916 |
Vacuole Periphery | 0.016302 | 0.007020 | 0.011008 | 0.004486 | 0.035524 | 0.006885 | 0.003435 | 0.005108 | 0.020340 | 0.006935 | 0.004685 | 0.004819 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -5.07 | -0.55 | -5.87 | 0.64 | -3.59 | -3.23 | -4.00 | -2.44 | 0.27 | 1.62 | -7.09 | -5.28 | -5.62 | 0.46 | 0.86 |
Bud Neck | -3.98 | -3.85 | -3.33 | 1.65 | 2.05 | -3.95 | -4.72 | -1.97 | 2.36 | 3.37 | -5.61 | -6.04 | -3.22 | 2.64 | 3.37 |
Bud Site | 0.57 | 15.31 | 0.21 | -0.34 | -12.56 | -6.45 | -1.39 | -3.22 | 3.72 | -2.38 | -0.27 | 8.96 | 1.60 | 1.94 | -7.57 |
Cell Periphery | -13.00 | -23.77* | -19.73 | -5.51 | 7.17 | 2.11 | -9.44 | -8.28 | -15.79 | 1.52 | -13.75 | -33.33 | -27.01 | -14.67 | 7.21 |
Cytoplasm | 10.02 | 26.08 | 5.15 | -4.41 | -18.07 | -18.28 | -10.13 | -15.80 | -1.09 | -7.14 | 7.92 | 15.27 | 4.15 | -4.04 | -12.69 |
Cytoplasmic Foci | 11.87 | 18.75 | 16.15 | 4.89 | -6.50 | -5.83 | 3.48 | 2.65 | 10.73 | -1.49 | 11.52 | 18.11 | 16.82 | 10.79 | -4.53 |
Eisosomes | -8.53 | -4.75 | -7.94 | 2.11 | 3.89 | -4.07 | -6.06 | -2.37 | 0.92 | 4.46 | -11.42 | -8.57 | -6.59 | 1.91 | 6.06 |
Endoplasmic Reticulum | 2.07 | 21.43 | 28.34 | 21.30 | 5.26 | 2.80 | 15.17 | 17.11 | 29.34 | 7.81 | 1.17 | 26.39 | 32.47 | 36.41 | 10.29 |
Endosome | 7.79 | 15.79 | 17.94 | 12.70 | 2.96 | 0.85 | 11.41 | 8.16 | 9.59 | -0.64 | 9.19 | 19.22 | 18.01 | 15.98 | 0.43 |
Golgi | 7.60 | 7.20 | 14.07 | 6.94 | 5.51 | 11.55 | 13.08 | 12.51 | 4.36 | -1.49 | 14.49 | 18.55 | 18.35 | 8.00 | 0.52 |
Lipid Particles | 0.22 | 2.36 | -0.71 | -0.83 | -2.70 | -3.65 | -4.11 | -3.20 | 0.35 | 1.10 | 0.77 | 1.31 | -0.02 | -0.81 | -1.36 |
Mitochondria | 0.10 | 14.16 | 9.58 | 5.68 | -4.93 | -1.15 | 1.95 | 1.32 | 5.23 | -2.50 | -2.27 | 7.24 | 5.42 | 7.31 | -2.34 |
Mitotic Spindle | -1.66 | 5.17 | -2.19 | -0.85 | -2.91 | 1.12 | 2.58 | 1.79 | 0.59 | -0.45 | -1.26 | 3.40 | -1.83 | -0.92 | -2.60 |
None | 3.12 | 4.02 | 3.28 | 0.44 | -7.38 | 0.14 | 1.81 | -0.08 | -0.23 | -2.17 | 3.31 | 3.76 | 3.33 | 0.06 | -4.28 |
Nuclear Periphery | -2.27 | -11.94 | -10.11 | -0.17 | 6.49 | 1.91 | -5.90 | -1.01 | -4.09 | 6.44 | -3.59 | -18.30 | -11.47 | -1.79 | 9.16 |
Nuclear Periphery Foci | 2.02 | 5.56 | 6.02 | 2.06 | 1.41 | 5.52 | 6.97 | 0.91 | -0.85 | -1.30 | 4.68 | 7.25 | 5.29 | 0.83 | -1.56 |
Nucleolus | 1.58 | 5.15 | 2.02 | 0.26 | -3.21 | -1.47 | 0.70 | -1.04 | 0.45 | -2.06 | 1.61 | 3.46 | 1.97 | 0.52 | -2.26 |
Nucleus | 0.10 | 2.32 | -6.82 | -6.42 | -9.81 | 0.71 | 0.16 | -0.03 | -6.00 | -1.46 | 1.03 | -0.46 | -3.44 | -9.23 | -6.49 |
Peroxisomes | 0.22 | 11.17 | 10.02 | 8.33 | -7.47 | -15.50 | -7.78 | -6.99 | 13.77 | 3.26 | -4.98 | 7.01 | 9.03 | 16.22 | 3.93 |
Vacuole | 1.08 | -16.89 | -18.87 | -18.83 | 7.82 | 4.19 | -9.75 | -8.98 | -20.14 | 0.85 | 5.14 | -20.16 | -21.23 | -28.12 | 1.82 |
Vacuole Periphery | 8.04 | 3.00 | 12.05 | 3.49 | 4.27 | 8.56 | 9.71 | 9.09 | 2.33 | -2.98 | 12.07 | 14.66 | 14.39 | 4.04 | -0.31 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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