Standard name
Human Ortholog
Description Component of the conserved oligomeric Golgi complex; a cytosolic tethering complex (Cog1p through Cog8p) that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05
Bud 0 0 0 0 0 0 0 – 0.05 0 0.06 0.06 0 0 0 0 0 0 0 0 0 0 0.05 0
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.6 0.67 0.67 0.68 0.64 0.39 0.35 – 0.19 0.22 0.14 0.2 0.73 0.82 0.76 0.19 0.12 0.12 0.25 0.31 0.19 0.19 0.23 0.26
Endoplasmic Reticulum 0 0 0 0 0 0 0 – 0 0 0 0 0.08 0.05 0 0.09 0.07 0.09 0 0 0 0 0 0
Endosome 0 0 0.09 0.06 0.09 0.09 0.12 – 0 0 0 0.05 0.07 0.06 0.14 0.36 0.46 0.47 0.14 0.05 0.12 0.08 0.08 0.07
Golgi 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0.09 0.19 0.13 0.08 0 0.09 0.09 0.07 0.08
Mitochondria 0.34 0.36 0.1 0.23 0.14 0.45 0.46 – 0.81 0.74 0.87 0.73 0.07 0 0 0.09 0.09 0.08 0.35 0.47 0.45 0.47 0.4 0.38
Nucleus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0.05 0.05 0 0.06 – 0 0 0 0 0 0 0 0.11 0 0 0 0 0 0 0 0.05
SpindlePole 0 0 0.11 0.06 0.11 0.07 0.08 – 0 0.06 0 0.06 0 0 0 0.11 0.12 0.22 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0.06 0.05 0.07 – 0 0 0 0 0 0 0 0.14 0.1 0.06 0.05 0.05 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 1 4 4 1 1 3 0 – 4 6 3 3 3 10 1 1 3 5 3 8 14 8 16 16
Bud 2 8 5 3 2 10 12 – 21 17 17 23 4 1 0 5 1 3 2 4 0 6 21 14
Bud Neck 0 0 4 1 1 4 2 – 1 2 0 0 0 0 0 0 0 0 1 1 0 1 1 0
Bud Site 0 0 0 0 0 1 1 – 1 1 0 1 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 3 1 1 3 6 – 0 3 2 5 0 3 2 2 3 0 0 1 0 0 1 1
Cytoplasm 71 220 172 168 162 116 190 – 74 95 41 76 107 309 307 28 16 29 38 113 69 54 98 92
Endoplasmic Reticulum 2 10 2 1 0 3 1 – 4 7 3 6 12 18 11 13 10 21 1 1 2 3 3 4
Endosome 4 0 22 15 24 27 65 – 8 12 7 18 11 24 55 54 63 115 21 17 45 23 34 23
Golgi 1 0 2 1 1 0 0 – 0 3 0 1 1 6 4 13 26 31 12 8 34 25 29 27
Mitochondria 40 117 25 56 35 136 251 – 313 319 257 284 11 9 13 14 12 20 53 176 165 134 175 135
Nucleus 0 0 0 0 0 3 3 – 1 0 2 2 1 0 0 0 3 3 0 1 0 1 3 0
Nuclear Periphery 0 0 1 0 0 1 0 – 1 0 0 1 0 0 1 2 2 3 0 0 0 0 2 1
Nucleolus 0 0 0 0 1 0 0 – 0 1 1 1 0 0 0 1 0 4 0 0 0 1 0 0
Peroxisomes 1 1 7 12 13 13 30 – 6 7 7 11 0 0 9 16 4 8 5 2 16 6 10 16
SpindlePole 0 1 29 15 28 21 44 – 11 26 7 25 3 8 12 16 16 55 1 7 8 7 17 12
Vac/Vac Membrane 1 2 5 3 15 15 36 – 13 11 12 14 0 5 18 21 14 14 7 20 5 6 14 7
Unique Cell Count 118 328 256 248 253 299 540 385 429 297 389 147 376 406 151 138 245 154 372 373 287 435 360
Labelled Cell Count 123 363 281 277 284 356 641 458 510 359 471 153 393 433 186 173 311 154 372 373 287 435 360


Golgi

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.3 3.4 5.4 5.0 5.8 4.5 4.8 – 4.3 4.4 4.3 4.6 4.8 5.3 5.1 5.4 6.4 6.1 6.7 6.6 7.0
Std Deviation (1e-4) 0.7 0.5 1.3 1.3 1.5 1.4 1.3 – 1.5 1.0 1.3 1.4 1.0 1.1 1.1 1.1 1.2 1.4 2.9 2.8 3.0
Intensity Change (Log2) – – – -0.11 0.11 -0.27 -0.19 – -0.35 -0.3 -0.32 -0.23 -0.16 -0.04 -0.1 -0.0 0.24 0.16 0.31 0.29 0.38


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – 0 0 0
Bud Neck – – – – – – – – – – – – 0 0 0
Bud Site – – – – – – – – – – – – 0 0 0
Cell Periphery – – – – – – – – – – – – 0 0 0
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – 0 0 0
Endosome – – – – – – – – – – – – 0 0 0
Golgi – – – – – – – – – – – – 0 0 0
Mitochondria – – – – – – – – – – – – 0 0 0
Nuclear Periphery – – – – – – – – – – – – 0 0 0
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – 0 0 0
Peroxisomes – – – – – – – – – – – – 0 0 0
SpindlePole – – – – – – – – – – – – 0 0 0
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – 0 0 0
Cytoplasm – – – – – – – – – – – – 0 0 0
Nucleus – – – – – – – – – – – – 0 0 0
Vacuole – – – – – – – – – – – – 4.8 3.6 2.2

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 3.1827 4.1151 3.79 3.4062 3.1565 4.1789 3.5888 2.8525 3.0221 2.2603 2.6647 3.1369 -0.3236 0.0001 -0.2295 -0.1174 -0.3953 -0.2171
Actin 0.0137 0.0138 0.0148 0.0028 0.0004 0.0189 0.0737 0.0014 0.0085 0.0008 0.0005 0.0126 0.0338 0.0054 0.017 0.0004 0.0008 0.0046
Bud 0.0002 0.0007 0.0008 0.0002 0.0001 0.0003 0.0005 0.0003 0.0008 0.0002 0.0001 0.0022 0.0005 0.0004 0.0012 0.0002 0.0001 0.0002
Bud Neck 0.0007 0.0007 0.0006 0.0003 0.0002 0.0024 0.0009 0.0003 0.0003 0.0002 0.0002 0.0013 0.0039 0.0005 0.003 0.0003 0.0002 0.0043
Bud Periphery 0.0004 0.0008 0.0015 0.0002 0.0001 0.0004 0.0005 0.0006 0.0016 0.0004 0.0001 0.0062 0.0006 0.0003 0.0015 0.0001 0.0001 0.0002
Bud Site 0.0007 0.0022 0.0024 0.0005 0.0001 0.0006 0.0033 0.0018 0.0023 0.0003 0.0003 0.0007 0.0055 0.0029 0.0047 0.0002 0.0001 0.0003
Cell Periphery 0.0011 0.0003 0.0006 0.0002 0.0001 0.0012 0.0003 0.0008 0.0005 0.0003 0 0.0004 0.0004 0.0002 0.0004 0.0001 0.0001 0.0001
Cytoplasm 0.0547 0.1186 0.08 0.0523 0.0411 0.0992 0.0856 0.1581 0.0751 0.0237 0.0252 0.0946 0.0778 0.1122 0.1121 0.1188 0.0982 0.1421
Cytoplasmic Foci 0.1747 0.1751 0.1593 0.1585 0.1983 0.1697 0.2219 0.16 0.1108 0.2148 0.2054 0.1635 0.1841 0.1845 0.1917 0.1384 0.2059 0.1892
Eisosomes 0.0014 0.0003 0.0006 0.0003 0.0001 0.0003 0.0005 0.0003 0.0003 0.0001 0 0.0002 0.0009 0.0001 0.0003 0 0 0.0001
Endoplasmic Reticulum 0.0035 0.0037 0.0053 0.0019 0.0035 0.0029 0.0199 0.0074 0.0064 0.0018 0.0013 0.0042 0.0103 0.006 0.0097 0.0103 0.0074 0.004
Endosome 0.1942 0.2055 0.2331 0.3274 0.3556 0.1892 0.157 0.1954 0.2259 0.1627 0.3531 0.192 0.2231 0.2934 0.2736 0.4408 0.2907 0.3011
Golgi 0.1415 0.1424 0.1506 0.1889 0.1142 0.1222 0.0576 0.0841 0.072 0.1329 0.1062 0.0916 0.1033 0.1442 0.109 0.0869 0.1074 0.1256
Lipid Particles 0.063 0.0132 0.0404 0.0098 0.0439 0.0551 0.0352 0.0083 0.031 0.0152 0.0813 0.028 0.0455 0.0213 0.0206 0.0036 0.0432 0.021
Mitochondria 0.0253 0.0421 0.0457 0.0139 0.0307 0.061 0.0097 0.0704 0.0441 0.101 0.0092 0.0295 0.0275 0.0405 0.0247 0.0078 0.0681 0.0579
None 0.2738 0.2445 0.2288 0.2124 0.1905 0.2197 0.1589 0.2887 0.3675 0.297 0.056 0.3508 0.2268 0.1604 0.2051 0.166 0.1585 0.117
Nuclear Periphery 0.0005 0.0007 0.0015 0.001 0.001 0.0005 0.0579 0.0011 0.0038 0.0006 0.0005 0.0005 0.0032 0.0022 0.0021 0.0057 0.0017 0.0012
Nucleolus 0.0004 0.0004 0.0004 0.0003 0.0002 0.0003 0.0008 0.0003 0.0005 0.0003 0.0001 0.0001 0.0025 0.0002 0.0002 0.0001 0.0001 0.0002
Nucleus 0.0004 0.0006 0.0007 0.0006 0.0004 0.0006 0.037 0.0006 0.0009 0.0006 0.0002 0.0003 0.0017 0.0006 0.0008 0.0008 0.0003 0.0004
Peroxisomes 0.0449 0.0255 0.025 0.0158 0.0111 0.0484 0.0626 0.0109 0.0248 0.04 0.1502 0.016 0.034 0.0144 0.0133 0.003 0.0102 0.021
Punctate Nuclear 0.0013 0.0011 0.0009 0.0009 0.0012 0.0012 0.0081 0.0007 0.0041 0.001 0.0005 0.0008 0.0076 0.0011 0.0013 0.0004 0.002 0.0009
Vacuole 0.0024 0.0057 0.0047 0.008 0.005 0.0043 0.0046 0.0065 0.0127 0.0043 0.0071 0.0027 0.0048 0.0063 0.0055 0.0116 0.003 0.0057
Vacuole Periphery 0.001 0.0019 0.0023 0.004 0.0023 0.0019 0.0036 0.0018 0.006 0.0017 0.0025 0.0018 0.0022 0.0029 0.0022 0.0045 0.0019 0.003

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 20.3604 9.3941 13.0702 18.7051 17.8827 13.1285 18.403 13.2221 16.7317 21.5096
Translational Efficiency 0.8855 1.3584 0.8256 0.6676 0.7398 1.3267 1.1594 1.0921 0.9393 0.9708

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
2017 830 445 567 2156 2206 237 1578 4173 3036 682 2145

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 670.98 707.06 919.18 871.92 683.57 785.29 950.57 817.58 677.48 763.90 930.09 831.94
Standard Deviation 84.19 107.72 115.04 127.26 74.66 88.65 106.92 118.18 79.66 100.49 113.28 123.00
Intensity Change Log 2 — 0.075563 0.454078 0.377926 — 0.200136 0.475704 0.258271 — 0.139772 0.465032 0.318783

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000542 0.001426 0.003983 0.002212 0.000987 0.000816 0.005000 0.004166 0.000772 0.000983 0.004336 0.003649
Bud Neck 0.014243 0.031053 0.007035 0.008200 0.013661 0.029188 0.004702 0.011941 0.013943 0.029698 0.006224 0.010952
Bud Site 0.004262 0.024096 0.017729 0.035600 0.006213 0.013664 0.008694 0.026552 0.005270 0.016516 0.014589 0.028943
Cell Periphery 0.000106 0.000171 0.000152 0.000136 0.000093 0.000154 0.000197 0.000132 0.000099 0.000159 0.000168 0.000133
Cytoplasm 0.221208* 0.251729* 0.220169* 0.195109* 0.243810* 0.329232* 0.152604* 0.314104* 0.232885* 0.308044* 0.196690* 0.282649*
Cytoplasmic Foci 0.403627* 0.345551* 0.168562 0.205629* 0.383480* 0.325535* 0.206174* 0.181129* 0.393218* 0.331007* 0.181632* 0.187605*
Eisosomes 0.000332 0.000208 0.000071 0.000096 0.000292 0.000252 0.000138 0.000116 0.000311 0.000240 0.000094 0.000111
Endoplasmic Reticulum 0.002317 0.002252 0.008544 0.003098 0.001354 0.000955 0.007750 0.002251 0.001819 0.001310 0.008268 0.002475
Endosome 0.043968 0.072235 0.057813 0.111742 0.035998 0.033002 0.110989 0.070136 0.039850 0.043727 0.076292 0.081134
Golgi 0.078113 0.033021 0.023924 0.026426 0.103544 0.041583 0.040416 0.037434 0.091252 0.039242 0.029655 0.034524
Lipid Particles 0.047304 0.014891 0.014987 0.013284 0.034295 0.020480 0.031954 0.016533 0.040583 0.018952 0.020883 0.015674
Mitochondria 0.044925 0.037129 0.002315 0.007834 0.046343 0.030213 0.001280 0.004567 0.045658 0.032104 0.001955 0.005431
Mitotic Spindle 0.005495 0.013601 0.134608* 0.075874 0.006169 0.011689 0.074302 0.048245 0.005843 0.012212 0.113651* 0.055548
None 0.002111 0.002603 0.003584 0.001699 0.001480 0.002292 0.001044 0.002781 0.001785 0.002377 0.002701 0.002495
Nuclear Periphery 0.000773 0.000601 0.004298 0.001531 0.000309 0.000352 0.002345 0.001999 0.000533 0.000420 0.003619 0.001875
Nuclear Periphery Foci 0.000539 0.000709 0.014319 0.005141 0.000462 0.000212 0.023084 0.003347 0.000499 0.000348 0.017365 0.003821
Nucleolus 0.001108 0.002880 0.002045 0.001359 0.000923 0.001389 0.002268 0.001720 0.001012 0.001796 0.002122 0.001624
Nucleus 0.056465 0.053248 0.197290* 0.060060 0.061055 0.081070 0.157004* 0.115238 0.058836 0.073464 0.183290* 0.100653
Peroxisomes 0.024691 0.027322 0.014259 0.030303 0.025934 0.025331 0.035878 0.035614 0.025333 0.025876 0.021771 0.034210
Vacuole 0.044213 0.078742 0.101991 0.207581* 0.030638 0.049509 0.131843 0.118339 0.037200 0.057501 0.112365 0.141929
Vacuole Periphery 0.003660 0.006531 0.002323 0.007088 0.002959 0.003082 0.002335 0.003657 0.003298 0.004025 0.002328 0.004564

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -1.94 -4.25 -4.32 -0.90 2.33 0.65 -2.90 -3.86 -3.89 0.72 -0.91 -5.02 -4.89 -3.97 1.40
Bud Neck -6.25 4.98 5.72 8.86 0.08 -8.82 7.44 1.15 9.65 -6.21 -10.87 7.77 3.13 12.68 -4.39
Bud Site -9.97 -4.44 -6.72 -0.67 -2.31 -5.87 -1.97 -7.82 -3.74 -6.21 -10.80 -4.56 -10.55 -3.47 -3.78
Cell Periphery -4.62 -3.65 -1.62 3.11 2.32 -4.42 -6.60 -2.18 2.43 4.75 -5.46 -6.81 -2.22 3.33 4.62
Cytoplasm -2.86 -0.19 0.16 2.31 0.28 -10.13 6.27 -7.64 1.35 -10.79 -11.49 3.32 -7.73 2.16 -8.23
Cytoplasmic Foci 6.17 25.28 28.39 17.91 -0.21 7.89 16.68 33.76 24.84 1.85 10.92 30.10 45.41 30.46 0.70
Eisosomes 3.62 8.86 8.13 5.39 -2.95 1.61 7.12 9.74 6.98 3.37 3.30 12.30 11.75 8.37 0.17
Endoplasmic Reticulum 0.11 -8.21 -3.78 -2.81 6.47 2.26 -6.70 -5.34 -7.18 5.60 2.48 -10.76 -5.01 -6.99 9.33
Endosome -6.24 -0.97 -4.50 0.13 -3.16 1.48 -5.91 -5.09 -6.06 3.85 -1.93 -5.22 -6.17 -4.58 0.94
Golgi 10.26 13.37 14.32 4.50 0.89 12.81 10.98 16.03 4.53 0.54 15.52 18.27 20.23 5.85 -0.22
Lipid Particles 11.94 12.84 14.64 2.03 1.90 6.61 1.91 10.00 4.11 4.30 12.91 10.91 16.92 4.55 3.54
Mitochondria 1.92 18.21 13.50 7.26 -1.28 5.47 19.13 17.81 14.43 -5.84 5.87 26.09 23.84 15.64 -3.23
Mitotic Spindle -3.01 -9.82 -7.80 -6.22 4.43 -3.24 -5.14 -10.38 -8.33 1.44 -4.51 -10.98 -13.06 -10.37 5.59
None -0.67 -0.75 1.20 1.40 1.09 -2.13 1.58 -2.24 -0.53 -3.09 -1.73 -0.70 -1.44 0.08 0.11
Nuclear Periphery 0.91 -6.98 -6.01 -7.61 4.04 -1.20 -8.91 -7.41 -7.26 0.15 1.28 -9.25 -8.16 -8.95 3.33
Nuclear Periphery Foci -1.31 -6.05 -7.18 -6.95 3.68 1.89 -4.72 -5.38 -5.99 4.03 1.52 -7.57 -7.85 -8.31 5.82
Nucleolus -1.83 -2.03 -0.88 1.46 1.34 -1.32 -2.41 -3.14 -1.86 1.38 -2.30 -3.13 -3.49 -0.11 1.48
Nucleus 0.43 -12.20 -4.66 -4.53 9.06 -5.38 -7.08 -14.57 -10.85 2.15 -5.26 -13.99 -15.63 -11.73 7.02
Peroxisomes -0.98 5.25 1.68 2.15 -2.32 0.32 -0.58 -0.48 -0.72 0.34 -0.33 2.76 0.02 0.26 -2.36
Vacuole -7.75 -10.14 -22.09 -18.08 -12.67 -8.24 -10.82 -25.46 -21.47 1.51 -9.72 -15.00 -31.99 -27.13 -6.13
Vacuole Periphery -4.13 4.39 -0.35 1.93 -1.93 -0.37 3.08 0.62 0.82 -1.37 -2.31 5.22 0.60 1.89 -2.20
Standard name
Human Ortholog
Description Component of the conserved oligomeric Golgi complex; a cytosolic tethering complex (Cog1p through Cog8p) that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments
Localization
Cell Percentages —
Cell Cycle Regulation No
Subcompartmental Group N/A

Cog7

Cog7


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Cog7-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available