Standard name | |
---|---|
Human Ortholog | |
Description | Nicotinic acid mononucleotide adenylyltransferase; catalyzes the transfer of the adenylyl moiety of ATP to nicotinamide mononucleotide to form NAD; involved in de novo and salvage synthesis of NAD(+); homolog of human NMNAT; NMA2 has a paralog, NMA1, that arose from the whole genome duplication |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3 | 0 | 0 | 0 |
Bud | 0 | 1 | 0 | 0 | 3 | 7 | 7 | 4 | 4 | 3 | 5 | 9 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 2 | 3 | 1 | 1 | 4 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 2 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3 | 2 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 2 | 0 | 0 | 0 | 1 | 2 | 0 | 7 | 2 | 1 | 2 | 0 | 0 | 0 | 2 | 6 | 5 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 253 | 399 | 389 | 242 | 155 | 228 | 269 | 170 | 208 | 183 | 163 | 145 | 171 | 243 | 243 | 351 | 299 | 246 | 261 | 391 | 397 | 151 | 180 | 183 |
Endoplasmic Reticulum | 0 | 2 | 0 | 0 | 0 | 0 | 2 | 2 | 8 | 2 | 1 | 3 | 0 | 0 | 0 | 9 | 11 | 7 | 0 | 1 | 4 | 0 | 0 | 2 |
Endosome | 1 | 0 | 6 | 2 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 1 | 10 | 1 | 2 | 1 | 4 | 8 | 3 | 3 | 2 | 4 |
Golgi | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 2 | 1 | 0 | 0 | 0 |
Mitochondria | 13 | 12 | 9 | 25 | 52 | 157 | 229 | 167 | 190 | 295 | 302 | 229 | 0 | 0 | 0 | 5 | 2 | 0 | 4 | 6 | 5 | 1 | 1 | 3 |
Nucleus | 2 | 4 | 5 | 7 | 4 | 12 | 41 | 31 | 25 | 30 | 24 | 36 | 4 | 6 | 2 | 17 | 7 | 11 | 11 | 27 | 25 | 10 | 19 | 14 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 2 | 1 | 4 | 3 | 1 | 2 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 1 | 0 | 0 | 1 |
Nucleolus | 0 | 0 | 0 | 0 | 2 | 1 | 2 | 2 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 2 | 1 | 0 | 3 | 2 |
Vac/Vac Membrane | 15 | 21 | 65 | 42 | 74 | 73 | 97 | 97 | 105 | 112 | 88 | 123 | 26 | 126 | 132 | 117 | 75 | 64 | 4 | 14 | 8 | 2 | 7 | 8 |
Unique Cell Count | 267 | 428 | 434 | 280 | 255 | 386 | 523 | 392 | 448 | 494 | 464 | 434 | 192 | 309 | 306 | 435 | 348 | 299 | 298 | 463 | 459 | 175 | 229 | 232 |
Labelled Cell Count | 284 | 442 | 475 | 318 | 291 | 482 | 650 | 477 | 550 | 632 | 588 | 551 | 202 | 377 | 389 | 502 | 402 | 334 | 298 | 463 | 459 | 175 | 229 | 232 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 5.0 | 6.1 | 5.9 | 5.4 | 5.7 | 5.0 | 4.9 | 5.1 | 4.9 | 4.8 | 4.6 | 4.9 | 9.0 | 8.9 | 8.9 | 8.1 | 7.4 | 7.4 | 8.4 | 8.6 | 9.3 |
Std Deviation (1e-4) | 0.6 | 0.9 | 0.8 | 0.8 | 1.6 | 1.1 | 1.1 | 1.1 | 0.9 | 0.9 | 0.9 | 0.9 | 1.4 | 1.5 | 1.3 | 1.2 | 1.5 | 1.5 | 1.3 | 1.5 | 1.8 |
Intensity Change (Log2) | – | – | – | -0.12 | -0.04 | -0.23 | -0.25 | -0.21 | -0.27 | -0.3 | -0.35 | -0.26 | 0.61 | 0.6 | 0.6 | 0.47 | 0.33 | 0.34 | 0.51 | 0.55 | 0.67 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | -1.3 | -9.0 | -10.1 | -12.7 | -14.2 | -13.7 | -16.4 | -16.8 | -17.0 | -0.2 | -4.1 | -3.9 | -3.7 | -1.6 | -2.9 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1.7 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 4.2 | 8.2 | 13.7 | 14.9 | 14.2 | 0 | 0 | 0 | 16.7 | 0 | 0 | 0 | -1.1 | 0 | 0 |
Nucleus | 0 | 0 | 2.0 | 4.8 | 4.7 | 3.6 | 3.9 | 3.4 | 5.0 | 0 | 0 | 0 | 2.6 | 1.0 | 2.3 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 4.4 | 1.5 | 1.5 | 3.5 | 3.2 | 3.0 | 1.6 | 4.8 | -0.5 | 7.9 | 8.5 | 4.3 | 2.4 | 2.2 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 4.1946 | 4.5418 | 4.2963 | 3.9336 | 3.9496 | 4.3173 | 2.8109 | 3.6585 | 3.6291 | 2.9946 | 3.5453 | 3.3582 | 2.0736 | 2.6546 | 2.4757 | 2.5925 | 2.1703 | 2.6825 |
Actin | 0.0048 | 0.0012 | 0.0069 | 0.0015 | 0.0008 | 0.0033 | 0.0044 | 0.0059 | 0.0057 | 0.0017 | 0.0007 | 0.0018 | 0.0009 | 0.0035 | 0.0024 | 0.0012 | 0.0019 | 0.0018 |
Bud | 0.0015 | 0.0018 | 0.0036 | 0.0011 | 0.0007 | 0.0012 | 0.0006 | 0.0027 | 0.0021 | 0.0007 | 0.0003 | 0.0007 | 0.0005 | 0.0036 | 0.0013 | 0.001 | 0.0076 | 0.0012 |
Bud Neck | 0.0068 | 0.0014 | 0.0019 | 0.0031 | 0.0012 | 0.0119 | 0.0045 | 0.0016 | 0.0044 | 0.0029 | 0.0036 | 0.0062 | 0.0005 | 0.0008 | 0.0073 | 0.0018 | 0.0104 | 0.0031 |
Bud Periphery | 0.0015 | 0.0006 | 0.0017 | 0.0006 | 0.0008 | 0.0012 | 0.0005 | 0.0017 | 0.0018 | 0.0004 | 0.0002 | 0.0007 | 0.0005 | 0.002 | 0.0008 | 0.0007 | 0.0052 | 0.0013 |
Bud Site | 0.0075 | 0.01 | 0.0091 | 0.0028 | 0.0004 | 0.0011 | 0.0016 | 0.0183 | 0.0115 | 0.0021 | 0.0004 | 0.0008 | 0.0009 | 0.0084 | 0.009 | 0.0009 | 0.0149 | 0.0008 |
Cell Periphery | 0.0002 | 0.0001 | 0.0002 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0003 | 0.0002 | 0.0001 | 0 | 0.0001 | 0.0001 | 0.0002 | 0.0001 | 0.0001 | 0.0004 | 0.0001 |
Cytoplasm | 0.5287 | 0.6203 | 0.4999 | 0.5553 | 0.4884 | 0.5237 | 0.6171 | 0.7371 | 0.6552 | 0.6611 | 0.6923 | 0.622 | 0.5625 | 0.6805 | 0.6509 | 0.6657 | 0.6827 | 0.6371 |
Cytoplasmic Foci | 0.0149 | 0.0188 | 0.0125 | 0.0102 | 0.0091 | 0.017 | 0.016 | 0.0131 | 0.0096 | 0.0186 | 0.0139 | 0.0167 | 0.0113 | 0.0154 | 0.0137 | 0.014 | 0.0141 | 0.015 |
Eisosomes | 0.0001 | 0 | 0.0001 | 0 | 0.0001 | 0 | 0 | 0 | 0.0001 | 0 | 0.0001 | 0 | 0 | 0.0001 | 0 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0.0064 | 0.0033 | 0.0041 | 0.0051 | 0.0032 | 0.0029 | 0.0052 | 0.0046 | 0.0047 | 0.0061 | 0.0051 | 0.0034 | 0.0057 | 0.0045 | 0.0048 | 0.0047 | 0.0052 | 0.0049 |
Endosome | 0.0108 | 0.0069 | 0.0067 | 0.0086 | 0.0059 | 0.009 | 0.0121 | 0.0059 | 0.006 | 0.009 | 0.0086 | 0.007 | 0.0107 | 0.0093 | 0.013 | 0.0123 | 0.0094 | 0.0128 |
Golgi | 0.0012 | 0.0005 | 0.001 | 0.0002 | 0.0001 | 0.0004 | 0.0014 | 0.0007 | 0.0011 | 0.0004 | 0.0003 | 0.0004 | 0.0004 | 0.0005 | 0.0007 | 0.0003 | 0.0002 | 0.0004 |
Lipid Particles | 0.0012 | 0.0002 | 0.0009 | 0.0002 | 0.0001 | 0.0002 | 0.0012 | 0.0002 | 0.0013 | 0.0004 | 0.0001 | 0.0002 | 0.0003 | 0.0005 | 0.0003 | 0.0002 | 0.0002 | 0.0002 |
Mitochondria | 0.0019 | 0.0009 | 0.0015 | 0.0013 | 0.0007 | 0.0011 | 0.0012 | 0.0009 | 0.0011 | 0.0009 | 0.0008 | 0.0009 | 0.0016 | 0.0007 | 0.001 | 0.001 | 0.0005 | 0.0011 |
None | 0.2245 | 0.1273 | 0.2087 | 0.0943 | 0.263 | 0.1505 | 0.1418 | 0.0816 | 0.132 | 0.0631 | 0.1034 | 0.1059 | 0.219 | 0.1767 | 0.103 | 0.0557 | 0.0836 | 0.0975 |
Nuclear Periphery | 0.0082 | 0.0097 | 0.0097 | 0.0193 | 0.0087 | 0.0096 | 0.0107 | 0.0056 | 0.0083 | 0.0123 | 0.0085 | 0.0074 | 0.0117 | 0.0065 | 0.012 | 0.011 | 0.0083 | 0.0089 |
Nucleolus | 0.0011 | 0.0013 | 0.0015 | 0.0026 | 0.0011 | 0.0016 | 0.0015 | 0.0013 | 0.0011 | 0.0017 | 0.0008 | 0.0014 | 0.0007 | 0.0006 | 0.0012 | 0.0008 | 0.0006 | 0.001 |
Nucleus | 0.1512 | 0.1576 | 0.1947 | 0.2338 | 0.1753 | 0.1941 | 0.1521 | 0.0959 | 0.1306 | 0.1588 | 0.1304 | 0.1831 | 0.1512 | 0.0708 | 0.149 | 0.2062 | 0.1202 | 0.1751 |
Peroxisomes | 0.0027 | 0.0015 | 0.0022 | 0.0005 | 0.0003 | 0.0008 | 0.0013 | 0.0015 | 0.0044 | 0.0009 | 0.0003 | 0.001 | 0.0003 | 0.0013 | 0.0009 | 0.0004 | 0.0003 | 0.0005 |
Punctate Nuclear | 0.0215 | 0.0342 | 0.0312 | 0.0559 | 0.0377 | 0.0682 | 0.023 | 0.0183 | 0.0162 | 0.0558 | 0.028 | 0.0384 | 0.0161 | 0.0102 | 0.0224 | 0.0171 | 0.0309 | 0.0328 |
Vacuole | 0.0029 | 0.002 | 0.0016 | 0.0027 | 0.0019 | 0.0015 | 0.003 | 0.0025 | 0.0023 | 0.0024 | 0.0018 | 0.0016 | 0.0041 | 0.0035 | 0.0051 | 0.0042 | 0.0027 | 0.0034 |
Vacuole Periphery | 0.0005 | 0.0004 | 0.0004 | 0.0007 | 0.0003 | 0.0005 | 0.0006 | 0.0003 | 0.0004 | 0.0005 | 0.0004 | 0.0003 | 0.0008 | 0.0005 | 0.001 | 0.0007 | 0.0005 | 0.0008 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 11.1455 | 12.4542 | 16.6349 | 15.1868 | 10.838 | 13.9242 | 18.2591 | 21.2477 | 22.9216 | 17.974 |
Translational Efficiency | 1.384 | 1.2124 | 0.7912 | 0.9932 | 1.371 | 1.295 | 1.0448 | 0.8913 | 0.8255 | 1.0282 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✔ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
940 | 290 | 1769 | 226 | 1245 | 1351 | 609 | 800 | 2185 | 1641 | 2378 | 1026 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 743.05 | 901.97 | 926.40 | 902.73 | 733.92 | 849.35 | 987.30 | 919.73 | 737.85 | 858.65 | 942.00 | 915.99 |
Standard Deviation | 119.32 | 115.46 | 119.53 | 117.60 | 119.36 | 180.32 | 131.35 | 130.17 | 119.43 | 171.83 | 125.51 | 127.70 |
Intensity Change Log 2 | — | 0.279620 | 0.318176 | 0.280835 | — | 0.210736 | 0.427866 | 0.325588 | — | 0.245802 | 0.373724 | 0.303247 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.000172 | 0.000537 | 0.000329 | 0.000354 | 0.000118 | 0.000178 | 0.000530 | 0.000995 | 0.000141 | 0.000241 | 0.000380 | 0.000854 |
Bud Neck | 0.002930 | 0.010404 | 0.006912 | 0.004540 | 0.001320 | 0.004257 | 0.003166 | 0.013868 | 0.002012 | 0.005344 | 0.005953 | 0.011814 |
Bud Site | 0.003716 | 0.015258 | 0.013381 | 0.023033 | 0.001680 | 0.007479 | 0.006747 | 0.033890 | 0.002556 | 0.008853 | 0.011682 | 0.031498 |
Cell Periphery | 0.000129 | 0.000178 | 0.000129 | 0.000189 | 0.000102 | 0.000115 | 0.000299 | 0.000183 | 0.000113 | 0.000126 | 0.000172 | 0.000184 |
Cytoplasm | 0.593616* | 0.517306* | 0.502915* | 0.425930* | 0.622577* | 0.569083* | 0.402005* | 0.478193* | 0.610118* | 0.559933* | 0.477072* | 0.466681* |
Cytoplasmic Foci | 0.087061 | 0.108604 | 0.006403 | 0.017678 | 0.036585 | 0.043797 | 0.006173 | 0.009875 | 0.058300 | 0.055250 | 0.006344 | 0.011594 |
Eisosomes | 0.000049 | 0.000065 | 0.000020 | 0.000061 | 0.000021 | 0.000029 | 0.000043 | 0.000038 | 0.000033 | 0.000035 | 0.000026 | 0.000043 |
Endoplasmic Reticulum | 0.002053 | 0.003098 | 0.002592 | 0.004582 | 0.001679 | 0.001310 | 0.005584 | 0.003464 | 0.001840 | 0.001626 | 0.003359 | 0.003710 |
Endosome | 0.004279 | 0.010939 | 0.001367 | 0.006456 | 0.001639 | 0.003826 | 0.002804 | 0.005471 | 0.002775 | 0.005083 | 0.001735 | 0.005688 |
Golgi | 0.000993 | 0.004794 | 0.000104 | 0.000938 | 0.000267 | 0.000801 | 0.003908 | 0.002125 | 0.000579 | 0.001507 | 0.001078 | 0.001863 |
Lipid Particles | 0.002996 | 0.002288 | 0.000698 | 0.001680 | 0.001256 | 0.001789 | 0.001276 | 0.001442 | 0.002004 | 0.001878 | 0.000846 | 0.001494 |
Mitochondria | 0.001640 | 0.002420 | 0.000929 | 0.002340 | 0.000942 | 0.001727 | 0.000813 | 0.002968 | 0.001242 | 0.001850 | 0.000900 | 0.002829 |
Mitotic Spindle | 0.000604 | 0.004068 | 0.012973 | 0.010600 | 0.001665 | 0.005739 | 0.004324 | 0.028796 | 0.001208 | 0.005444 | 0.010758 | 0.024788 |
None | 0.006805 | 0.002790 | 0.006243 | 0.014706 | 0.010218 | 0.006367 | 0.010350 | 0.011785 | 0.008750 | 0.005735 | 0.007295 | 0.012428 |
Nuclear Periphery | 0.000534 | 0.000669 | 0.001879 | 0.001443 | 0.000985 | 0.001501 | 0.001520 | 0.001586 | 0.000791 | 0.001354 | 0.001787 | 0.001554 |
Nuclear Periphery Foci | 0.002112 | 0.003611 | 0.001450 | 0.003006 | 0.001313 | 0.001175 | 0.003507 | 0.001707 | 0.001657 | 0.001605 | 0.001977 | 0.001993 |
Nucleolus | 0.001348 | 0.001678 | 0.000264 | 0.000590 | 0.000617 | 0.000966 | 0.000303 | 0.000559 | 0.000931 | 0.001092 | 0.000274 | 0.000566 |
Nucleus | 0.249346* | 0.218677* | 0.385233* | 0.284459* | 0.300391* | 0.304761* | 0.463860* | 0.307120* | 0.278431* | 0.289548* | 0.405369* | 0.302129* |
Peroxisomes | 0.000909 | 0.002773 | 0.000128 | 0.001162 | 0.000471 | 0.001627 | 0.000176 | 0.000566 | 0.000659 | 0.001830 | 0.000141 | 0.000698 |
Vacuole | 0.038158 | 0.088235 | 0.055529 | 0.193763 | 0.015837 | 0.042576 | 0.082423 | 0.094850 | 0.025440 | 0.050645 | 0.062417 | 0.116638 |
Vacuole Periphery | 0.000551 | 0.001608 | 0.000521 | 0.002490 | 0.000319 | 0.000897 | 0.000189 | 0.000521 | 0.000419 | 0.001023 | 0.000436 | 0.000954 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -4.11 | -3.38 | -4.96 | 1.57 | -0.78 | -1.86 | -9.46 | -5.55 | -4.78 | 0.28 | -3.21 | -7.34 | -5.97 | -4.41 | -1.85 |
Bud Neck | -2.74 | -4.39 | -1.58 | 1.98 | 1.88 | -3.53 | -4.96 | -4.83 | -2.65 | -2.74 | -3.87 | -6.86 | -4.72 | -1.85 | -1.27 |
Bud Site | -3.12 | -3.25 | -1.70 | 0.43 | -0.52 | -4.39 | -4.26 | -5.65 | -3.22 | -3.73 | -4.70 | -5.25 | -5.56 | -2.75 | -2.86 |
Cell Periphery | -1.99 | 0.82 | -0.36 | 1.63 | -1.01 | -0.74 | -2.27 | -3.56 | -1.61 | 1.42 | -0.86 | -2.00 | -2.93 | -1.03 | 0.10 |
Cytoplasm | 4.62 | 15.50 | 13.63 | 7.90 | 5.56 | 6.01 | 25.54 | 24.51 | 19.14 | -2.02 | 6.60 | 27.63 | 27.95 | 20.79 | 6.47 |
Cytoplasmic Foci | -1.77 | 19.09 | 17.24 | 10.76 | -3.12 | -2.33 | 15.82 | 14.99 | 16.17 | -1.72 | 1.28 | 23.84 | 22.49 | 18.74 | -3.23 |
Eisosomes | -2.27 | 5.94 | -3.39 | -0.14 | -8.49 | -3.69 | -11.57 | -12.04 | -8.47 | 1.26 | -1.23 | -1.42 | -9.07 | -7.39 | -9.12 |
Endoplasmic Reticulum | -2.51 | -5.16 | -4.52 | -2.56 | -2.86 | 1.16 | -11.25 | -8.88 | -11.44 | 5.30 | 0.55 | -11.70 | -9.86 | -10.67 | -1.10 |
Endosome | -3.92 | 6.58 | 0.76 | 3.81 | -1.94 | -4.73 | -3.01 | -2.32 | 1.92 | 0.08 | -4.53 | 4.08 | -0.20 | 3.65 | -2.83 |
Golgi | -1.52 | 2.64 | 1.44 | 1.81 | -1.03 | -1.88 | -0.89 | -1.45 | 0.48 | 0.63 | -1.77 | 0.34 | 0.10 | 1.77 | -0.27 |
Lipid Particles | 1.44 | 5.35 | 2.88 | 1.83 | -4.30 | -1.87 | -1.80 | -1.12 | 1.01 | 0.67 | 0.49 | 4.36 | 1.88 | 1.40 | -3.42 |
Mitochondria | -0.53 | 1.03 | 0.46 | 0.92 | -0.40 | -1.13 | 0.22 | -1.03 | 0.27 | -2.01 | -0.97 | 0.69 | -0.50 | 0.62 | -1.78 |
Mitotic Spindle | -2.00 | -6.92 | -3.09 | -1.75 | 0.93 | -2.21 | -1.67 | -5.92 | -4.68 | -5.23 | -2.94 | -6.55 | -6.59 | -4.90 | -3.13 |
None | 4.32 | 1.46 | -0.73 | -2.09 | -1.22 | 2.66 | 2.03 | 2.05 | -0.99 | -0.11 | 3.00 | 3.21 | 0.91 | -1.91 | -1.91 |
Nuclear Periphery | -1.68 | -19.86 | -5.91 | -5.42 | 0.60 | -0.80 | -2.64 | -3.20 | -3.23 | -1.89 | -1.28 | -5.62 | -5.70 | -4.07 | -0.67 |
Nuclear Periphery Foci | -1.96 | 1.01 | -0.86 | 0.55 | -1.23 | 0.43 | -7.16 | -3.48 | -3.13 | 3.60 | 0.10 | -2.80 | -2.44 | -2.18 | -0.46 |
Nucleolus | -0.37 | 3.09 | 2.61 | 1.37 | -1.92 | -1.29 | 3.31 | 1.49 | 1.76 | -1.87 | -0.53 | 4.01 | 2.88 | 2.24 | -3.14 |
Nucleus | 1.88 | -17.50 | -4.40 | -5.19 | 4.67 | -0.72 | -14.11 | -6.06 | -5.52 | 7.80 | -1.72 | -21.27 | -8.63 | -6.90 | 7.55 |
Peroxisomes | -3.45 | 6.11 | 1.30 | 3.83 | -1.53 | -2.24 | 1.86 | 1.42 | 2.90 | -1.13 | -2.70 | 4.95 | 2.81 | 3.70 | -2.45 |
Vacuole | -6.30 | -11.51 | -12.18 | -8.17 | -9.26 | -11.02 | -16.17 | -21.89 | -17.40 | -4.61 | -9.87 | -21.96 | -24.07 | -19.32 | -12.81 |
Vacuole Periphery | -1.14 | 1.14 | -0.49 | 0.54 | -0.83 | -2.35 | 2.18 | 0.91 | 2.72 | -2.00 | -2.28 | 1.13 | 0.12 | 2.06 | -0.65 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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