Standard name | |
---|---|
Human Ortholog | |
Description | Putative cysteine synthase; localized to the mitochondrial outer membrane |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 2 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 2 | 6 | 3 | 1 | 3 | 4 | 5 | 10 | 13 |
Bud | 5 | 2 | 5 | 5 | 3 | 9 | 24 | 26 | 17 | 22 | 15 | 13 | 1 | 1 | 5 | 1 | 6 | 4 | 1 | 2 | 1 | 3 | 8 | 9 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 4 | 2 |
Bud Site | 0 | 1 | 2 | 1 | 1 | 1 | 7 | 19 | 4 | 11 | 6 | 2 | 0 | 0 | 0 | 0 | 2 | 1 | – | – | – | – | – | – |
Cell Periphery | 5 | 4 | 13 | 4 | 5 | 3 | 8 | 3 | 0 | 2 | 4 | 2 | 18 | 29 | 17 | 10 | 20 | 10 | 0 | 0 | 1 | 0 | 0 | 0 |
Cytoplasm | 1 | 3 | 5 | 10 | 20 | 13 | 16 | 15 | 8 | 16 | 13 | 17 | 37 | 52 | 81 | 12 | 12 | 8 | 0 | 0 | 0 | 3 | 7 | 6 |
Endoplasmic Reticulum | 4 | 4 | 0 | 0 | 3 | 1 | 7 | 5 | 0 | 2 | 0 | 2 | 0 | 0 | 1 | 1 | 10 | 4 | 0 | 1 | 0 | 2 | 4 | 3 |
Endosome | 3 | 5 | 6 | 13 | 22 | 11 | 8 | 9 | 15 | 0 | 0 | 0 | 40 | 93 | 101 | 0 | 10 | 4 | 13 | 12 | 10 | 12 | 18 | 27 |
Golgi | 1 | 8 | 4 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 24 | 81 | 60 | 27 | 60 | 35 | 6 | 13 | 8 | 23 | 27 | 44 |
Mitochondria | 217 | 157 | 238 | 189 | 272 | 355 | 529 | 649 | 314 | 530 | 350 | 425 | 195 | 212 | 240 | 3 | 15 | 7 | 179 | 134 | 204 | 209 | 301 | 385 |
Nucleus | 0 | 3 | 4 | 2 | 6 | 6 | 17 | 18 | 11 | 7 | 5 | 6 | 3 | 2 | 1 | 2 | 8 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
Nuclear Periphery | 13 | 6 | 13 | 2 | 12 | 16 | 36 | 34 | 9 | 23 | 13 | 20 | 4 | 4 | 2 | 4 | 3 | 2 | 4 | 1 | 3 | 2 | 2 | 3 |
Nucleolus | 13 | 8 | 7 | 3 | 7 | 14 | 31 | 39 | 15 | 30 | 15 | 16 | 2 | 2 | 3 | 3 | 4 | 2 | 2 | 0 | 0 | 2 | 2 | 2 |
Peroxisomes | 2 | 2 | 24 | 15 | 15 | 7 | 16 | 14 | 8 | 2 | 6 | 2 | 41 | 52 | 99 | 2 | 7 | 7 | 6 | 5 | 35 | 7 | 15 | 19 |
SpindlePole | 0 | 3 | 11 | 5 | 6 | 15 | 22 | 29 | 19 | 14 | 16 | 11 | 12 | 13 | 16 | 0 | 6 | 3 | 3 | 1 | 5 | 0 | 5 | 4 |
Vac/Vac Membrane | 23 | 26 | 12 | 6 | 33 | 32 | 35 | 33 | 28 | 27 | 16 | 18 | 88 | 161 | 109 | 21 | 44 | 24 | 6 | 11 | 0 | 17 | 33 | 27 |
Unique Cell Count | 225 | 180 | 268 | 210 | 328 | 405 | 618 | 738 | 367 | 567 | 375 | 449 | 338 | 514 | 528 | 73 | 181 | 98 | 230 | 192 | 280 | 294 | 449 | 556 |
Labelled Cell Count | 287 | 234 | 345 | 256 | 405 | 483 | 757 | 893 | 449 | 686 | 459 | 535 | 465 | 703 | 736 | 88 | 213 | 114 | 230 | 192 | 280 | 294 | 449 | 556 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 5.1 | 6.9 | 6.4 | 6.1 | 6.1 | 5.4 | 5.1 | 4.9 | 5.2 | 4.5 | 4.1 | 4.3 | 7.9 | 7.6 | 7.4 | 10.5 | 9.8 | 10.5 | 8.0 | 7.6 | 7.9 |
Std Deviation (1e-4) | 1.1 | 1.6 | 1.8 | 1.8 | 2.2 | 1.8 | 1.8 | 1.7 | 2.0 | 1.6 | 1.6 | 1.6 | 1.7 | 1.5 | 1.6 | 2.2 | 1.9 | 2.2 | 1.7 | 1.8 | 1.9 |
Intensity Change (Log2) | – | – | – | -0.08 | -0.09 | -0.25 | -0.33 | -0.38 | -0.31 | -0.52 | -0.64 | -0.6 | 0.29 | 0.23 | 0.2 | 0.7 | 0.61 | 0.7 | 0.32 | 0.24 | 0.29 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0.3 | 1.6 | 1.3 | 1.9 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 1.8 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | -1.7 | -2.4 | -3.4 | -3.2 | 0 | -4.3 | 0 | 0 | 0 | 0.3 | 0.5 | -1.1 | 0 | 2.5 | 1.9 |
Cytoplasm | 1.8 | 2.6 | 1.1 | 0.7 | 0.2 | 0.3 | 0.8 | 1.2 | 1.4 | 4.4 | 4.2 | 5.8 | 0 | 2.6 | 0 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 2.2 | 2.6 | 0.4 | 0 | 0 | 1.3 | 0 | 0 | 0 | 4.4 | 6.3 | 6.6 | 0 | 1.8 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3.3 | 6.1 | 4.8 | 9.4 | 9.4 | 9.4 |
Mitochondria | 0.4 | -2.0 | -0.5 | -1.3 | -0.4 | -1.2 | 0 | 0 | 0 | -8.4 | -12.8 | -11.8 | -14.1 | -16.9 | -14.7 |
Nucleus | 0 | 0 | 0 | 1.1 | 0.9 | 1.2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | -2.4 | -0.7 | -0.6 | 0.6 | -0.2 | -1.6 | 0 | 0 | 0 | -2.7 | -3.7 | -4.4 | 0 | -1.8 | 0 |
Nucleolus | 0 | -0.4 | 0.6 | 1.6 | 1.8 | 1.0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | -0.7 | -2.2 | -4.4 | -4.2 | -5.2 | -3.9 | 0 | 0 | 0 | 1.3 | 0.5 | 3.6 | -1.8 | -2.1 | -0.6 |
SpindlePole | -1.0 | -1.7 | -0.3 | -0.4 | -0.1 | 0.6 | 0 | 0 | 0 | -0.4 | -1.2 | -0.8 | 0 | -0.4 | 0 |
Vacuole | -0.9 | 2.6 | 1.8 | 0.7 | 0 | 1.6 | 0.2 | -0.1 | -0.3 | 7.1 | 8.6 | 6.0 | 6.2 | 6.2 | 5.7 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | -0.6169 | -0.0197 | -0.6216 | -0.5821 | -0.9239 | -0.2854 | 1.4213 | 1.6162 | 1.67 | 1.1531 | 1.6487 | 1.6672 | 1.074 | 1.1432 | 1.0677 | 1.7693 | 1.5477 | 1.0601 |
Actin | 0.0471 | 0.0064 | 0.0111 | 0.0043 | 0.003 | 0.0045 | 0.0243 | 0.014 | 0.0119 | 0.0715 | 0.0605 | 0.0044 | 0.011 | 0.0192 | 0.0152 | 0.067 | 0.0144 | 0.0062 |
Bud | 0.0009 | 0.0034 | 0.0018 | 0.0035 | 0.0008 | 0.0008 | 0.0011 | 0.004 | 0.0019 | 0.0024 | 0.0047 | 0.0013 | 0.0029 | 0.007 | 0.0089 | 0.0037 | 0.0029 | 0.0011 |
Bud Neck | 0.001 | 0.0004 | 0.012 | 0.0012 | 0.0028 | 0.0047 | 0.0023 | 0.0009 | 0.003 | 0.0039 | 0.0029 | 0.0048 | 0.0007 | 0.0011 | 0.0012 | 0.0036 | 0.0021 | 0.0056 |
Bud Periphery | 0.0023 | 0.0125 | 0.0072 | 0.0093 | 0.0037 | 0.0016 | 0.0041 | 0.009 | 0.005 | 0.0072 | 0.0209 | 0.0041 | 0.0088 | 0.0136 | 0.0139 | 0.0069 | 0.0058 | 0.0044 |
Bud Site | 0.004 | 0.0132 | 0.0126 | 0.0076 | 0.0013 | 0.0008 | 0.0049 | 0.0223 | 0.0091 | 0.0076 | 0.005 | 0.0014 | 0.0046 | 0.0318 | 0.0152 | 0.0088 | 0.0021 | 0.0012 |
Cell Periphery | 0.001 | 0.0005 | 0.0011 | 0.0014 | 0.0006 | 0.0004 | 0.0018 | 0.0027 | 0.0016 | 0.0023 | 0.0014 | 0.001 | 0.001 | 0.0013 | 0.0014 | 0.0005 | 0.0003 | 0.0005 |
Cytoplasm | 0.0105 | 0.0063 | 0.0071 | 0.0199 | 0.0041 | 0.0077 | 0.0312 | 0.0274 | 0.0383 | 0.0182 | 0.0094 | 0.028 | 0.0253 | 0.0222 | 0.0225 | 0.0124 | 0.0308 | 0.0145 |
Cytoplasmic Foci | 0.0538 | 0.0533 | 0.0421 | 0.0624 | 0.0452 | 0.0521 | 0.074 | 0.0726 | 0.0467 | 0.0301 | 0.0448 | 0.0512 | 0.054 | 0.0692 | 0.0531 | 0.078 | 0.044 | 0.0562 |
Eisosomes | 0.0021 | 0.0002 | 0.0014 | 0.0009 | 0.0003 | 0.0003 | 0.0007 | 0.0008 | 0.0007 | 0.0012 | 0.0015 | 0.0003 | 0.0004 | 0.001 | 0.0003 | 0.0003 | 0.0002 | 0.0003 |
Endoplasmic Reticulum | 0.0039 | 0.0003 | 0.0033 | 0.0017 | 0.0002 | 0.002 | 0.0047 | 0.0056 | 0.0035 | 0.0041 | 0.0035 | 0.0022 | 0.0023 | 0.0021 | 0.0023 | 0.0009 | 0.0033 | 0.0016 |
Endosome | 0.055 | 0.0392 | 0.0404 | 0.0486 | 0.0234 | 0.0625 | 0.1314 | 0.121 | 0.0905 | 0.0924 | 0.1078 | 0.0726 | 0.1026 | 0.1061 | 0.077 | 0.1059 | 0.106 | 0.0762 |
Golgi | 0.0263 | 0.0169 | 0.0167 | 0.0346 | 0.0492 | 0.0232 | 0.0498 | 0.0525 | 0.0434 | 0.0352 | 0.0261 | 0.0447 | 0.0403 | 0.0599 | 0.0383 | 0.064 | 0.0616 | 0.0555 |
Lipid Particles | 0.0599 | 0.0413 | 0.0653 | 0.0066 | 0.0414 | 0.0371 | 0.0484 | 0.02 | 0.0181 | 0.0109 | 0.0076 | 0.0199 | 0.0162 | 0.0087 | 0.0135 | 0.0129 | 0.0106 | 0.0127 |
Mitochondria | 0.5286 | 0.6908 | 0.5942 | 0.5981 | 0.6767 | 0.6599 | 0.4652 | 0.5134 | 0.6395 | 0.6529 | 0.6228 | 0.6736 | 0.6212 | 0.5313 | 0.6369 | 0.5843 | 0.6384 | 0.6761 |
None | 0.1117 | 0.0662 | 0.0991 | 0.0615 | 0.0333 | 0.0556 | 0.0814 | 0.0626 | 0.0457 | 0.0068 | 0.0263 | 0.0272 | 0.0502 | 0.0725 | 0.0309 | 0.0177 | 0.0161 | 0.0276 |
Nuclear Periphery | 0.0035 | 0.0002 | 0.0011 | 0.0003 | 0.0001 | 0.0044 | 0.0045 | 0.003 | 0.0014 | 0.0005 | 0.0075 | 0.0084 | 0.0067 | 0.0013 | 0.0033 | 0.0004 | 0.015 | 0.0007 |
Nucleolus | 0.0059 | 0.0033 | 0.0042 | 0.0011 | 0.0011 | 0.0038 | 0.0034 | 0.0017 | 0.0042 | 0.0009 | 0.0029 | 0.0007 | 0.0008 | 0.0012 | 0.0009 | 0.0005 | 0.0009 | 0.0005 |
Nucleus | 0.0049 | 0.0005 | 0.002 | 0.0007 | 0.0004 | 0.0025 | 0.0026 | 0.0029 | 0.0026 | 0.0009 | 0.0051 | 0.0012 | 0.0023 | 0.0014 | 0.0017 | 0.0006 | 0.0084 | 0.0006 |
Peroxisomes | 0.0543 | 0.0364 | 0.0615 | 0.1169 | 0.1062 | 0.0599 | 0.0283 | 0.0278 | 0.0145 | 0.0333 | 0.0173 | 0.037 | 0.0267 | 0.0313 | 0.0403 | 0.0208 | 0.0174 | 0.0484 |
Punctate Nuclear | 0.0092 | 0.0013 | 0.0082 | 0.0003 | 0.0004 | 0.0034 | 0.0052 | 0.0016 | 0.0019 | 0.0005 | 0.0044 | 0.0013 | 0.0026 | 0.0007 | 0.0033 | 0.0008 | 0.0086 | 0.0006 |
Vacuole | 0.0098 | 0.0042 | 0.0055 | 0.0146 | 0.0033 | 0.0079 | 0.021 | 0.0233 | 0.011 | 0.0121 | 0.0128 | 0.0091 | 0.0111 | 0.012 | 0.0143 | 0.0057 | 0.0065 | 0.0058 |
Vacuole Periphery | 0.0043 | 0.0032 | 0.0024 | 0.0045 | 0.0026 | 0.0049 | 0.0095 | 0.0109 | 0.0054 | 0.0051 | 0.0049 | 0.0056 | 0.0083 | 0.0051 | 0.0058 | 0.0042 | 0.0046 | 0.004 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 22.8709 | 26.7039 | 19.1962 | 18.2831 | 19.3653 | 27.0567 | 21.094 | 17.3692 | 19.9167 | 25.5097 |
Translational Efficiency | 0.9764 | 0.658 | 0.7349 | 0.797 | 1.0897 | 0.688 | 0.8101 | 1.0163 | 0.8589 | 0.9553 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
1647 | 647 | 1923 | 809 | 2003 | 1530 | 1061 | 912 | 3650 | 2177 | 2984 | 1721 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 730.07 | 874.23 | 1003.25 | 928.76 | 752.67 | 836.20 | 1039.11 | 1000.94 | 742.47 | 847.50 | 1016.00 | 967.01 |
Standard Deviation | 106.04 | 402.99 | 161.72 | 180.37 | 120.26 | 154.86 | 166.48 | 194.23 | 114.62 | 255.78 | 164.33 | 191.26 |
Intensity Change Log 2 | — | 0.259978 | 0.458574 | 0.347271 | — | 0.151831 | 0.465259 | 0.411266 | — | 0.206093 | 0.461972 | 0.380111 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.000587 | 0.000778 | 0.003815 | 0.007364 | 0.000865 | 0.002270 | 0.003321 | 0.009600 | 0.000739 | 0.001827 | 0.003639 | 0.008549 |
Bud Neck | 0.006762 | 0.012410 | 0.002807 | 0.007654 | 0.003564 | 0.009273 | 0.006846 | 0.006774 | 0.005007 | 0.010205 | 0.004243 | 0.007187 |
Bud Site | 0.012849 | 0.028686 | 0.025068 | 0.087468 | 0.022263 | 0.030430 | 0.041894 | 0.102326* | 0.018015 | 0.029912 | 0.031051 | 0.095342 |
Cell Periphery | 0.000796 | 0.000668 | 0.000515 | 0.002000 | 0.000698 | 0.000787 | 0.000414 | 0.002648 | 0.000742 | 0.000752 | 0.000479 | 0.002343 |
Cytoplasm | 0.019380 | 0.018865 | 0.006368 | 0.027151 | 0.014022 | 0.023584 | 0.013287 | 0.039723 | 0.016439 | 0.022181 | 0.008828 | 0.033813 |
Cytoplasmic Foci | 0.044347 | 0.056858 | 0.029780 | 0.031203 | 0.035225 | 0.049724 | 0.047370 | 0.040980 | 0.039341 | 0.051844 | 0.036034 | 0.036384 |
Eisosomes | 0.000086 | 0.000104 | 0.000061 | 0.000134 | 0.000061 | 0.000094 | 0.000078 | 0.000120 | 0.000072 | 0.000097 | 0.000067 | 0.000126 |
Endoplasmic Reticulum | 0.001227 | 0.000797 | 0.001515 | 0.001576 | 0.000798 | 0.000862 | 0.001140 | 0.001356 | 0.000992 | 0.000843 | 0.001381 | 0.001459 |
Endosome | 0.067812 | 0.099820 | 0.042464 | 0.043799 | 0.051807 | 0.064430 | 0.053107 | 0.043301 | 0.059029 | 0.074948 | 0.046248 | 0.043535 |
Golgi | 0.059569 | 0.066274 | 0.068742 | 0.061051 | 0.077802 | 0.067239 | 0.068960 | 0.088815 | 0.069575 | 0.066952 | 0.068820 | 0.075764 |
Lipid Particles | 0.008091 | 0.007424 | 0.000986 | 0.001027 | 0.002257 | 0.003483 | 0.001687 | 0.000982 | 0.004889 | 0.004654 | 0.001235 | 0.001003 |
Mitochondria | 0.690712* | 0.579512* | 0.696220* | 0.555649* | 0.727634* | 0.634604* | 0.574566* | 0.488749* | 0.710974* | 0.618230* | 0.652965* | 0.520197* |
Mitotic Spindle | 0.006907 | 0.020485 | 0.041462 | 0.046173 | 0.009956 | 0.019163 | 0.065778 | 0.054499 | 0.008580 | 0.019556 | 0.050108 | 0.050585 |
None | 0.002387 | 0.002977 | 0.000491 | 0.000671 | 0.000996 | 0.002047 | 0.000568 | 0.000595 | 0.001623 | 0.002323 | 0.000518 | 0.000630 |
Nuclear Periphery | 0.000467 | 0.000376 | 0.000651 | 0.000519 | 0.000090 | 0.000233 | 0.000932 | 0.000177 | 0.000260 | 0.000276 | 0.000751 | 0.000338 |
Nuclear Periphery Foci | 0.000425 | 0.000914 | 0.000646 | 0.000760 | 0.000241 | 0.000485 | 0.000665 | 0.000315 | 0.000324 | 0.000613 | 0.000652 | 0.000524 |
Nucleolus | 0.001176 | 0.002773 | 0.000407 | 0.000348 | 0.000323 | 0.000801 | 0.000514 | 0.000275 | 0.000708 | 0.001387 | 0.000445 | 0.000309 |
Nucleus | 0.002804 | 0.005823 | 0.001837 | 0.003995 | 0.000727 | 0.006753 | 0.002704 | 0.002074 | 0.001664 | 0.006476 | 0.002146 | 0.002977 |
Peroxisomes | 0.020135 | 0.030537 | 0.010896 | 0.027623 | 0.015585 | 0.030636 | 0.031703 | 0.026901 | 0.017638 | 0.030606 | 0.018295 | 0.027240 |
Vacuole | 0.025489 | 0.038357 | 0.030729 | 0.052168 | 0.015073 | 0.028762 | 0.045081 | 0.052895 | 0.019773 | 0.031614 | 0.035832 | 0.052553 |
Vacuole Periphery | 0.027993 | 0.025563 | 0.034539 | 0.041668 | 0.020013 | 0.024340 | 0.039385 | 0.036895 | 0.023614 | 0.024703 | 0.036262 | 0.039139 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -1.16 | -4.71 | -4.99 | -4.85 | -2.30 | -4.35 | -5.36 | -5.47 | -4.51 | -3.75 | -4.71 | -6.18 | -7.37 | -6.22 | -4.19 |
Bud Neck | -2.86 | 4.89 | -0.91 | 2.12 | -4.65 | -4.84 | -4.07 | -4.40 | 1.47 | -0.15 | -5.04 | 1.15 | -3.46 | 2.28 | -4.47 |
Bud Site | -4.25 | -5.50 | -11.74 | -8.16 | -9.47 | -2.78 | -5.79 | -12.38 | -10.72 | -8.54 | -5.24 | -6.76 | -16.97 | -13.43 | -13.47 |
Cell Periphery | 0.70 | 1.53 | -1.98 | -2.23 | -2.49 | -1.07 | 3.30 | -3.07 | -2.88 | -3.55 | -0.28 | 2.47 | -3.63 | -3.58 | -4.25 |
Cytoplasm | 0.06 | 5.43 | -2.99 | -2.77 | -6.63 | -3.51 | -0.53 | -6.98 | -4.29 | -6.54 | -2.75 | 4.20 | -7.32 | -4.74 | -9.83 |
Cytoplasmic Foci | -2.51 | 5.01 | 3.53 | 5.11 | -0.82 | -4.88 | -3.68 | -1.90 | 2.31 | 1.52 | -4.82 | 1.64 | 1.01 | 5.23 | -0.41 |
Eisosomes | -2.00 | 4.61 | -2.21 | -1.27 | -3.40 | -6.41 | -3.80 | -6.94 | -2.78 | -4.44 | -5.32 | 1.30 | -4.94 | -2.54 | -5.33 |
Endoplasmic Reticulum | 1.47 | -0.75 | -1.20 | -3.15 | -0.31 | -0.39 | -2.30 | -3.00 | -2.86 | -1.12 | 0.84 | -1.66 | -2.66 | -4.05 | -0.44 |
Endosome | -4.96 | 7.16 | 5.85 | 8.76 | -0.25 | -3.52 | -0.32 | 2.80 | 5.80 | 2.94 | -4.98 | 5.41 | 6.10 | 9.85 | 1.37 |
Golgi | -1.13 | -2.07 | 0.00 | 1.00 | 1.69 | 2.40 | 1.93 | -1.25 | -3.04 | -2.68 | 0.81 | 0.37 | -0.90 | -1.51 | -1.18 |
Lipid Particles | 0.41 | 7.22 | 6.92 | 4.41 | -0.40 | -1.96 | 0.96 | 2.72 | 3.86 | 1.36 | 0.24 | 7.17 | 7.38 | 5.87 | 0.62 |
Mitochondria | 6.55 | -0.33 | 9.24 | 1.79 | 9.64 | 8.12 | 11.65 | 17.17 | 10.07 | 5.43 | 9.85 | 7.25 | 18.82 | 9.05 | 12.46 |
Mitotic Spindle | -4.58 | -12.68 | -9.11 | -5.01 | -0.85 | -5.03 | -12.11 | -9.26 | -7.12 | 1.50 | -7.16 | -17.20 | -12.87 | -8.89 | -0.21 |
None | -0.47 | 2.99 | 2.69 | 2.02 | -3.30 | -2.39 | 2.59 | 3.51 | 3.34 | -0.09 | -1.39 | 3.73 | 3.39 | 3.74 | -1.96 |
Nuclear Periphery | 0.50 | -1.12 | -0.27 | -0.77 | 0.69 | -3.25 | -2.77 | -3.81 | 0.54 | 2.37 | -0.24 | -3.55 | -0.95 | -0.87 | 2.71 |
Nuclear Periphery Foci | -1.86 | -2.11 | -1.50 | 0.45 | -0.50 | -1.94 | -3.53 | -1.92 | 0.84 | 2.27 | -2.43 | -4.30 | -2.08 | 0.38 | 0.78 |
Nucleolus | -1.77 | 2.56 | 2.81 | 2.76 | 0.34 | -2.48 | -1.69 | 0.37 | 2.64 | 1.93 | -2.18 | 1.62 | 2.73 | 3.62 | 1.36 |
Nucleus | -1.92 | 0.93 | -1.83 | 0.36 | -2.30 | -5.59 | -3.50 | -4.24 | 2.09 | 0.31 | -5.32 | -2.07 | -3.88 | 1.71 | -1.94 |
Peroxisomes | -2.88 | 4.36 | -1.93 | 0.59 | -4.60 | -5.51 | -4.84 | -3.59 | 0.97 | 1.11 | -5.90 | -0.35 | -3.89 | 1.10 | -3.52 |
Vacuole | -3.66 | -2.66 | -7.55 | -4.07 | -6.15 | -7.18 | -10.18 | -10.43 | -7.27 | -2.97 | -6.83 | -9.90 | -12.90 | -8.76 | -7.08 |
Vacuole Periphery | 0.88 | -2.69 | -3.47 | -3.78 | -1.65 | -2.24 | -6.11 | -4.93 | -3.39 | 0.81 | -0.65 | -6.84 | -6.00 | -5.23 | -0.71 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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