Standard name | |
---|---|
Human Ortholog | |
Description | Highly-conserved N-terminally anchored AAA-ATPase; distributed in the mitochondrial outer membrane and peroxisomes; involved in mitochondrial protein sorting; functions as an extraction engine in local organelle surveillance to remove and initiate degradation of mistargeted proteins, ensuring fidelity of organelle-specific localization of tail-anchored proteins; contains an N-terminal transmembrane domain and C-terminal cytoplasmic ATPase domain |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 4 | 4 | 3 | 1 | 1 | 2 | 0 | 1 | – | 0 | 0 | 3 | 4 | 22 | 5 | 2 | 0 | 2 | 3 | 6 | 8 | 6 | 18 | 31 |
Bud | 2 | 3 | 27 | 18 | 18 | 34 | 22 | 25 | – | 24 | 43 | 37 | 2 | 3 | 4 | 7 | 2 | 1 | 0 | 0 | 0 | 0 | 0 | 2 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 2 |
Bud Site | 0 | 0 | 0 | 1 | 1 | 4 | 13 | 12 | – | 5 | 9 | 12 | 0 | 0 | 0 | 1 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 3 | 2 | 4 | 12 | 4 | 6 | 2 | 5 | – | 7 | 6 | 5 | 3 | 6 | 4 | 5 | 0 | 3 | 0 | 0 | 1 | 0 | 0 | 0 |
Cytoplasm | 4 | 3 | 12 | 18 | 33 | 23 | 22 | 22 | – | 24 | 18 | 13 | 5 | 4 | 12 | 10 | 2 | 8 | 1 | 0 | 1 | 0 | 0 | 1 |
Endoplasmic Reticulum | 8 | 14 | 11 | 11 | 9 | 18 | 9 | 14 | – | 19 | 6 | 9 | 0 | 29 | 16 | 5 | 1 | 5 | 1 | 1 | 1 | 2 | 3 | 6 |
Endosome | 3 | 0 | 0 | 4 | 13 | 2 | 1 | 6 | – | 0 | 2 | 0 | 10 | 3 | 18 | 11 | 1 | 3 | 12 | 10 | 23 | 10 | 13 | 14 |
Golgi | 13 | 11 | 2 | 3 | 3 | 0 | 0 | 0 | – | 3 | 0 | 1 | 20 | 45 | 64 | 27 | 13 | 23 | 26 | 19 | 20 | 15 | 30 | 40 |
Mitochondria | 170 | 100 | 296 | 334 | 529 | 644 | 825 | 851 | – | 778 | 731 | 917 | 62 | 72 | 92 | 7 | 3 | 4 | 131 | 80 | 214 | 106 | 135 | 148 |
Nucleus | 3 | 0 | 1 | 1 | 3 | 7 | 9 | 14 | – | 3 | 4 | 4 | 0 | 1 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 6 | 0 | 4 | 2 | 14 | 17 | 27 | 33 | – | 44 | 17 | 34 | 0 | 3 | 1 | 0 | 2 | 0 | 1 | 0 | 0 | 2 | 1 | 1 |
Nucleolus | 1 | 0 | 0 | 0 | 8 | 21 | 38 | 56 | – | 17 | 16 | 22 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 0 |
Peroxisomes | 3 | 0 | 5 | 6 | 6 | 1 | 2 | 9 | – | 4 | 5 | 4 | 8 | 4 | 11 | 12 | 3 | 3 | 14 | 4 | 34 | 12 | 21 | 17 |
SpindlePole | 1 | 0 | 2 | 0 | 5 | 7 | 3 | 10 | – | 7 | 11 | 4 | 2 | 0 | 2 | 3 | 1 | 2 | 1 | 0 | 1 | 0 | 0 | 1 |
Vac/Vac Membrane | 21 | 8 | 11 | 8 | 41 | 30 | 56 | 63 | – | 60 | 47 | 39 | 22 | 18 | 32 | 14 | 7 | 8 | 3 | 3 | 2 | 5 | 8 | 11 |
Unique Cell Count | 193 | 114 | 315 | 360 | 586 | 688 | 865 | 915 | 833 | 762 | 944 | 98 | 153 | 203 | 87 | 32 | 54 | 201 | 130 | 315 | 170 | 241 | 283 | |
Labelled Cell Count | 242 | 145 | 378 | 419 | 688 | 817 | 1029 | 1121 | 995 | 915 | 1104 | 138 | 210 | 261 | 105 | 36 | 63 | 201 | 130 | 315 | 170 | 241 | 283 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 6.3 | 6.2 | 4.7 | 4.7 | 5.0 | 4.9 | 4.5 | 4.8 | – | 4.8 | 4.9 | 4.8 | 7.6 | 7.3 | 7.3 | 7.5 | 7.2 | 7.5 | 7.7 | 7.9 | 8.2 |
Std Deviation (1e-4) | 0.8 | 1.4 | 1.1 | 1.0 | 1.2 | 1.2 | 1.1 | 1.2 | – | 1.2 | 1.2 | 1.1 | 1.6 | 1.0 | 0.9 | 1.7 | 1.3 | 1.6 | 1.6 | 1.6 | 1.7 |
Intensity Change (Log2) | – | – | – | -0.02 | 0.07 | 0.06 | -0.09 | 0.02 | – | 0.01 | 0.04 | 0.02 | 0.68 | 0.62 | 0.62 | 0.66 | 0.59 | 0.67 | 0.7 | 0.74 | 0.79 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 6.1 | 0 | 0 | 0 | 0 |
Bud | 0 | -3.6 | -2.2 | 0 | 0 | – | 0 | -1.8 | 0 | -2.2 | -2.7 | -3.1 | -0.2 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 0.7 | 1.2 | -0.4 | -1.2 | -1.3 | – | -0.8 | -1.3 | -2.7 | 0 | -0.7 | 1.1 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0 | -1.9 | -0.8 | 0 | 0 | – | 0 | 0 | 0 | 0 | 5.6 | 2.2 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 5.4 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 7.6 | 9.7 | 10.3 | 9.7 | 0 | 0 |
Mitochondria | 0 | -1.9 | -0.2 | 0 | 0 | – | 0 | 1.4 | 0 | -7.8 | -11.6 | -12.5 | -16.5 | -13.2 | -15.1 |
Nucleus | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 1.1 | 1.2 | 0 | 0 | – | 0 | 1.0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 3.1 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 2.5 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | -1.0 | 2.2 | 0.6 | 2.0 | 2.2 | – | 2.3 | 1.8 | 0.5 | 6.0 | 3.5 | 4.9 | 4.3 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 0.7445 | 1.0998 | 1.0615 | 0.8986 | 0.5764 | 0.9894 | 2.7758 | 3.2722 | 3.3231 | 3.2799 | 2.3701 | 3.4259 | 0.8996 | 1.1005 | 0.9475 | 1.0469 | 0.8425 | 0.9481 |
Actin | 0.0294 | 0.0349 | 0.0213 | 0.0065 | 0.0018 | 0.0126 | 0.0256 | 0.0138 | 0.0201 | 0.0502 | 0.0191 | 0.0222 | 0.0232 | 0.0259 | 0.0274 | 0.011 | 0.0085 | 0.0162 |
Bud | 0.0007 | 0.0028 | 0.0011 | 0.0033 | 0.0039 | 0.0004 | 0.0006 | 0.0009 | 0.0012 | 0.0018 | 0.0005 | 0.0008 | 0.0006 | 0.0011 | 0.0007 | 0.0008 | 0.0047 | 0.0004 |
Bud Neck | 0.0017 | 0.0012 | 0.0028 | 0.0006 | 0.0008 | 0.0008 | 0.0007 | 0.001 | 0.0014 | 0.0006 | 0.0024 | 0.0043 | 0.0018 | 0.0028 | 0.0016 | 0.0212 | 0.0071 | 0.0016 |
Bud Periphery | 0.0015 | 0.0053 | 0.0038 | 0.0057 | 0.0052 | 0.0007 | 0.0009 | 0.0014 | 0.0027 | 0.0033 | 0.0011 | 0.002 | 0.0011 | 0.0013 | 0.0011 | 0.0024 | 0.0074 | 0.0008 |
Bud Site | 0.005 | 0.0121 | 0.0085 | 0.0075 | 0.0172 | 0.0005 | 0.0018 | 0.0122 | 0.0042 | 0.0011 | 0.0015 | 0.0017 | 0.0037 | 0.0124 | 0.0059 | 0.0018 | 0.0197 | 0.0005 |
Cell Periphery | 0.0015 | 0.0005 | 0.0017 | 0.0004 | 0.0012 | 0.0002 | 0.0003 | 0.0004 | 0.0015 | 0.0002 | 0.0003 | 0.0007 | 0.0007 | 0.0003 | 0.0007 | 0.0009 | 0.0013 | 0.0002 |
Cytoplasm | 0.0094 | 0.0042 | 0.0054 | 0.0016 | 0.0018 | 0.0046 | 0.0034 | 0.0096 | 0.01 | 0.0031 | 0.0021 | 0.0069 | 0.0053 | 0.0081 | 0.0038 | 0.0017 | 0.0045 | 0.0015 |
Cytoplasmic Foci | 0.0587 | 0.062 | 0.0426 | 0.0196 | 0.0562 | 0.0273 | 0.0467 | 0.0534 | 0.0374 | 0.0536 | 0.0382 | 0.0431 | 0.0636 | 0.0631 | 0.0329 | 0.0411 | 0.0454 | 0.0251 |
Eisosomes | 0.0043 | 0.0005 | 0.0017 | 0.0002 | 0.0004 | 0.0003 | 0.0005 | 0.0004 | 0.0027 | 0.0003 | 0.0005 | 0.0006 | 0.001 | 0.0002 | 0.001 | 0.0002 | 0.0002 | 0.0003 |
Endoplasmic Reticulum | 0.0013 | 0.0006 | 0.0007 | 0.0004 | 0.0002 | 0.0009 | 0.0012 | 0.0012 | 0.0008 | 0.0004 | 0.0003 | 0.001 | 0.0017 | 0.0014 | 0.0018 | 0.0001 | 0.0055 | 0.0009 |
Endosome | 0.1031 | 0.1177 | 0.051 | 0.0278 | 0.0654 | 0.0566 | 0.1282 | 0.0943 | 0.0639 | 0.085 | 0.0856 | 0.0785 | 0.094 | 0.1065 | 0.0677 | 0.0485 | 0.126 | 0.0658 |
Golgi | 0.095 | 0.147 | 0.08 | 0.0351 | 0.0722 | 0.075 | 0.1672 | 0.1459 | 0.0989 | 0.0895 | 0.1811 | 0.1116 | 0.1129 | 0.1299 | 0.1006 | 0.0702 | 0.1142 | 0.1048 |
Lipid Particles | 0.0442 | 0.0122 | 0.0187 | 0.004 | 0.0357 | 0.0165 | 0.0286 | 0.0153 | 0.0093 | 0.0107 | 0.043 | 0.0081 | 0.0297 | 0.0197 | 0.0096 | 0.0034 | 0.0082 | 0.0165 |
Mitochondria | 0.4955 | 0.5087 | 0.6553 | 0.7703 | 0.6535 | 0.7306 | 0.537 | 0.5711 | 0.6628 | 0.6066 | 0.5895 | 0.6268 | 0.5561 | 0.544 | 0.6669 | 0.7276 | 0.5852 | 0.701 |
None | 0.0265 | 0.0012 | 0.0219 | 0.0003 | 0.0021 | 0.0067 | 0.0132 | 0.0188 | 0.0171 | 0.0028 | 0.0007 | 0.015 | 0.0179 | 0.0124 | 0.0079 | 0.0003 | 0.0019 | 0.0042 |
Nuclear Periphery | 0.0057 | 0.0002 | 0.0013 | 0.0003 | 0.0002 | 0.0003 | 0.0004 | 0.0007 | 0.0002 | 0.0001 | 0.0003 | 0.0017 | 0.0042 | 0.001 | 0.001 | 0 | 0.0066 | 0.0005 |
Nucleolus | 0.0032 | 0.0004 | 0.0011 | 0.0001 | 0.0015 | 0.0004 | 0.0003 | 0.0002 | 0.0013 | 0.0002 | 0.0004 | 0.0003 | 0.0007 | 0.0006 | 0.0006 | 0.0001 | 0.0006 | 0.0001 |
Nucleus | 0.0016 | 0.0005 | 0.0008 | 0.0003 | 0.0005 | 0.0012 | 0.0003 | 0.0005 | 0.001 | 0.0002 | 0.0002 | 0.0005 | 0.0007 | 0.0008 | 0.0003 | 0.0001 | 0.001 | 0.0002 |
Peroxisomes | 0.0947 | 0.0769 | 0.0729 | 0.1099 | 0.0708 | 0.0596 | 0.0341 | 0.0532 | 0.0551 | 0.0845 | 0.029 | 0.0659 | 0.0722 | 0.0584 | 0.0604 | 0.0661 | 0.0375 | 0.054 |
Punctate Nuclear | 0.0022 | 0.0002 | 0.0023 | 0.0001 | 0.004 | 0.0005 | 0.0003 | 0.0002 | 0.0006 | 0.0008 | 0.0013 | 0.0019 | 0.0015 | 0.0003 | 0.0001 | 0.0001 | 0.0023 | 0.0002 |
Vacuole | 0.0102 | 0.0066 | 0.0028 | 0.0044 | 0.003 | 0.0021 | 0.0049 | 0.0034 | 0.0059 | 0.0025 | 0.0013 | 0.0034 | 0.0037 | 0.0057 | 0.0045 | 0.0011 | 0.0065 | 0.002 |
Vacuole Periphery | 0.0044 | 0.004 | 0.002 | 0.0016 | 0.0022 | 0.0023 | 0.0038 | 0.0021 | 0.002 | 0.0025 | 0.0015 | 0.0029 | 0.0035 | 0.0042 | 0.0036 | 0.0012 | 0.0059 | 0.0031 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 10.1323 | 11.7749 | 13.4423 | 16.2033 | 13.1369 | 14.1773 | 21.7506 | 19.1615 | 17.2618 | 21.3857 |
Translational Efficiency | 1.382 | 0.974 | 0.9489 | 0.9139 | 0.9551 | 1.3719 | 0.8612 | 0.9105 | 1.356 | 0.696 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✔ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
1757 | 1629 | 744 | 1271 | 1938 | 1762 | 2474 | 1885 | 3695 | 3391 | 3218 | 3156 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 737.15 | 841.10 | 1055.72 | 970.19 | 757.21 | 756.47 | 979.91 | 1028.75 | 747.67 | 797.13 | 997.44 | 1005.17 |
Standard Deviation | 94.95 | 117.51 | 147.28 | 148.65 | 95.53 | 115.43 | 119.64 | 150.56 | 95.78 | 123.87 | 130.54 | 152.52 |
Intensity Change Log 2 | — | 0.190319 | 0.518197 | 0.396309 | — | -0.001411 | 0.371956 | 0.442127 | — | 0.096351 | 0.445948 | 0.419707 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.001851 | 0.005679 | 0.017569 | 0.136583* | 0.006730 | 0.006078 | 0.009636 | 0.069240 | 0.004410 | 0.005886 | 0.011470 | 0.096361 |
Bud Neck | 0.001637 | 0.001913 | 0.001917 | 0.002567 | 0.001444 | 0.003175 | 0.000657 | 0.001960 | 0.001536 | 0.002569 | 0.000948 | 0.002205 |
Bud Site | 0.005894 | 0.010800 | 0.014962 | 0.086680 | 0.007137 | 0.009375 | 0.015740 | 0.067726 | 0.006546 | 0.010060 | 0.015560 | 0.075360 |
Cell Periphery | 0.000570 | 0.000625 | 0.000791 | 0.004832 | 0.000704 | 0.000594 | 0.000387 | 0.003146 | 0.000640 | 0.000609 | 0.000480 | 0.003825 |
Cytoplasm | 0.003101 | 0.003075 | 0.001871 | 0.013997 | 0.002373 | 0.003736 | 0.003548 | 0.015746 | 0.002719 | 0.003419 | 0.003160 | 0.015041 |
Cytoplasmic Foci | 0.056388 | 0.063156 | 0.105775 | 0.067641 | 0.043846 | 0.057266 | 0.045034 | 0.066642 | 0.049810 | 0.060095 | 0.059078 | 0.067045 |
Eisosomes | 0.000135 | 0.000177 | 0.000179 | 0.000661 | 0.000242 | 0.000222 | 0.000138 | 0.000579 | 0.000191 | 0.000200 | 0.000148 | 0.000612 |
Endoplasmic Reticulum | 0.001325 | 0.000810 | 0.004515 | 0.006691 | 0.000721 | 0.000647 | 0.000929 | 0.003324 | 0.001008 | 0.000725 | 0.001758 | 0.004680 |
Endosome | 0.086005 | 0.098494 | 0.099693 | 0.070974 | 0.082648 | 0.091429 | 0.067108 | 0.074180 | 0.084244 | 0.094823 | 0.074641 | 0.072889 |
Golgi | 0.219313* | 0.227789* | 0.352799* | 0.211840* | 0.195806* | 0.220963* | 0.188200* | 0.246887* | 0.206984* | 0.224242* | 0.226255* | 0.232773* |
Lipid Particles | 0.006982 | 0.004087 | 0.003023 | 0.000978 | 0.002879 | 0.006049 | 0.000283 | 0.000504 | 0.004830 | 0.005107 | 0.000916 | 0.000695 |
Mitochondria | 0.561954* | 0.526281* | 0.294068* | 0.308692* | 0.612608* | 0.538327* | 0.595588* | 0.375695* | 0.588522* | 0.532540* | 0.525877* | 0.348711* |
Mitotic Spindle | 0.002424 | 0.008381 | 0.019254 | 0.021932 | 0.002503 | 0.004248 | 0.016422 | 0.011017 | 0.002465 | 0.006234 | 0.017077 | 0.015413 |
None | 0.000333 | 0.000229 | 0.000334 | 0.000244 | 0.000173 | 0.000214 | 0.000200 | 0.000193 | 0.000249 | 0.000221 | 0.000231 | 0.000213 |
Nuclear Periphery | 0.000140 | 0.000082 | 0.000786 | 0.000189 | 0.000037 | 0.000064 | 0.000126 | 0.000043 | 0.000086 | 0.000073 | 0.000279 | 0.000102 |
Nuclear Periphery Foci | 0.000233 | 0.000233 | 0.001756 | 0.000756 | 0.000139 | 0.000218 | 0.000160 | 0.000131 | 0.000184 | 0.000225 | 0.000529 | 0.000383 |
Nucleolus | 0.000780 | 0.000261 | 0.000101 | 0.000163 | 0.000168 | 0.001113 | 0.000023 | 0.000020 | 0.000459 | 0.000704 | 0.000041 | 0.000078 |
Nucleus | 0.000587 | 0.000351 | 0.000141 | 0.000192 | 0.000163 | 0.000677 | 0.000061 | 0.000187 | 0.000365 | 0.000520 | 0.000079 | 0.000189 |
Peroxisomes | 0.018996 | 0.021083 | 0.010084 | 0.027772 | 0.018500 | 0.037117 | 0.012629 | 0.032145 | 0.018736 | 0.029414 | 0.012041 | 0.030384 |
Vacuole | 0.009818 | 0.010505 | 0.030863 | 0.019851 | 0.006563 | 0.007782 | 0.019047 | 0.016129 | 0.008111 | 0.009090 | 0.021779 | 0.017628 |
Vacuole Periphery | 0.021533 | 0.015985 | 0.039517 | 0.016765 | 0.014616 | 0.010708 | 0.024084 | 0.014504 | 0.017905 | 0.013243 | 0.027653 | 0.015414 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -4.55 | -6.57 | -19.41 | -18.76 | -16.24 | 0.57 | -2.17 | -15.91 | -16.14 | -14.98 | -2.04 | -6.52 | -24.93 | -24.45 | -22.41* |
Bud Neck | -0.72 | -0.74 | -2.56 | -2.30 | -2.00 | -3.42 | 3.08 | -1.86 | 2.62 | -9.09 | -3.22 | 2.73 | -2.98 | 1.30 | -9.60 |
Bud Site | -3.52 | -4.50 | -19.09 | -17.70 | -15.81 | -1.83 | -7.07 | -18.95 | -18.00 | -16.02 | -3.81 | -8.88 | -26.84 | -25.11 | -22.67 |
Cell Periphery | -0.37 | -1.45 | -7.69 | -7.55 | -7.24 | 0.68 | 2.13 | -5.72 | -6.27 | -6.87 | 0.28 | 1.67 | -9.44 | -9.69 | -10.23 |
Cytoplasm | 0.03 | 2.30 | -9.80 | -10.20 | -11.64 | -2.68 | -2.74 | -13.49 | -11.42 | -12.00 | -1.82 | -1.17 | -16.47 | -15.35 | -15.80 |
Cytoplasmic Foci | -2.49 | -10.47 | -3.57 | -1.37 | 7.50 | -5.83 | -0.55 | -9.19 | -3.52 | -8.78 | -5.79 | -4.73 | -8.79 | -3.36 | -3.65 |
Eisosomes | -2.73 | -3.20 | -16.22 | -14.04 | -14.34 | 0.87 | 4.20 | -9.74 | -11.61 | -13.76 | -0.67 | 2.72 | -17.62 | -17.85 | -19.19 |
Endoplasmic Reticulum | 1.53 | -4.64 | -6.47 | -7.61 | -2.21 | 0.41 | -1.87 | -4.64 | -4.64 | -4.28 | 1.52 | -3.41 | -7.65 | -8.53 | -6.09 |
Endosome | -2.83 | -2.90 | 3.77 | 6.38 | 6.19 | -2.20 | 4.79 | 2.52 | 4.63 | -2.41 | -3.56 | 3.67 | 4.42 | 7.78 | 0.73 |
Golgi | -1.03 | -11.31 | 0.86 | 1.81 | 11.44 | -3.33 | 1.13 | -6.62 | -3.22 | -8.03 | -3.10 | -3.30 | -4.36 | -1.39 | -1.02 |
Lipid Particles | 2.53 | 3.70 | 6.35 | 4.66 | 3.64 | -2.67 | 4.92 | 4.46 | 5.20 | -2.90 | -0.35 | 7.29 | 7.82 | 6.90 | 1.41 |
Mitochondria | 3.01 | 18.53 | 20.59 | 17.33 | -0.95 | 6.63 | 1.66 | 22.00 | 14.55 | 21.36* | 6.86 | 7.36 | 29.53 | 22.02 | 20.38* |
Mitotic Spindle | -5.95 | -6.60 | -8.44 | -5.49 | -0.85 | -2.43 | -11.17 | -7.59 | -5.60 | 3.40 | -6.18 | -13.16 | -11.27 | -7.37 | 1.00 |
None | 1.66 | 0.01 | 1.71 | -0.29 | 3.44 | -1.23 | -1.41 | -0.94 | 0.71 | 0.58 | 0.80 | 0.74 | 1.34 | 0.37 | 1.55 |
Nuclear Periphery | 1.93 | -2.40 | -0.76 | -1.80 | 2.18 | -1.53 | -3.06 | -0.92 | 1.18 | 2.84 | 0.79 | -2.87 | -0.58 | -1.12 | 2.52 |
Nuclear Periphery Foci | -0.02 | -2.32 | -1.49 | -1.49 | 1.35 | -1.04 | -0.51 | 0.18 | 1.24 | 1.05 | -0.88 | -2.23 | -1.40 | -1.07 | 0.71 |
Nucleolus | 1.24 | 1.61 | 1.47 | 0.69 | -0.32 | -1.76 | 1.38 | 1.41 | 2.07 | 0.40 | -0.73 | 2.06 | 1.86 | 2.23 | -0.67 |
Nucleus | 0.96 | 2.04 | 1.83 | 1.22 | -0.67 | -1.58 | 1.75 | -0.40 | 1.46 | -1.91 | -0.75 | 2.67 | 1.49 | 1.79 | -2.35 |
Peroxisomes | -0.89 | 4.11 | -3.03 | -2.29 | -6.41 | -6.22 | 3.00 | -4.89 | 1.45 | -7.64 | -5.54 | 4.53 | -5.75 | -0.43 | -9.60 |
Vacuole | -0.63 | -8.72 | -5.42 | -4.96 | 3.80 | -1.33 | -11.57 | -7.37 | -5.88 | 1.87 | -1.38 | -13.54 | -8.79 | -7.52 | 3.03 |
Vacuole Periphery | 3.68 | -5.98 | 2.66 | -0.52 | 7.57 | 3.54 | -6.76 | 0.10 | -3.48 | 6.91 | 5.04 | -7.47 | 2.39 | -2.43 | 9.55 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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