Standard name | |
---|---|
Human Ortholog | |
Description | Protein involved in GPI anchor synthesis; multimembrane-spanning protein that localizes to the endoplasmic reticulum; highly conserved among eukaryotes; GPI stands for glycosylphosphatidylinositol |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 1 | 1 | 3 | 6 | 6 | – | 6 | 0 | 2 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 3 | 1 | 2 | 6 | 11 | – | 12 | 15 | 9 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 4 | 1 | 0 | 2 | 1 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 1 | 0 | 0 | 0 | 1 | 4 | 3 | 6 | – | 8 | 7 | 10 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 24 | 63 | 128 | 207 | 277 | 326 | 465 | 388 | – | 534 | 350 | 400 | 63 | 63 | 89 | 102 | 187 | 98 | 4 | 14 | 9 | 2 | 9 | 10 |
Endoplasmic Reticulum | 3 | 10 | 28 | 26 | 29 | 49 | 45 | 55 | – | 41 | 16 | 54 | 27 | 33 | 23 | 6 | 10 | 3 | 21 | 23 | 84 | 29 | 32 | 22 |
Endosome | 9 | 0 | 14 | 15 | 7 | 5 | 15 | 9 | – | 9 | 4 | 3 | 1 | 1 | 2 | 5 | 15 | 3 | 2 | 4 | 15 | 2 | 16 | 12 |
Golgi | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 1 | 1 | 0 | 1 | 0 | 2 | 0 | 0 | 1 | 1 | 1 | 1 |
Mitochondria | 0 | 1 | 4 | 3 | 1 | 5 | 8 | 7 | – | 9 | 9 | 6 | 1 | 1 | 0 | 7 | 12 | 1 | 0 | 2 | 5 | 0 | 3 | 2 |
Nucleus | 3 | 0 | 3 | 1 | 0 | 1 | 2 | 8 | – | 1 | 0 | 4 | 3 | 3 | 5 | 14 | 21 | 12 | 0 | 1 | 0 | 0 | 1 | 1 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 4 | – | 4 | 1 | 4 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 2 | 5 | 2 | 5 | 4 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 1 | – | 2 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vac/Vac Membrane | 61 | 67 | 127 | 90 | 79 | 45 | 106 | 74 | – | 65 | 38 | 46 | 70 | 66 | 51 | 116 | 266 | 141 | 92 | 116 | 185 | 100 | 255 | 244 |
Unique Cell Count | 83 | 131 | 266 | 299 | 371 | 418 | 596 | 521 | 638 | 400 | 490 | 152 | 151 | 162 | 202 | 429 | 224 | 126 | 172 | 313 | 143 | 332 | 307 | |
Labelled Cell Count | 101 | 142 | 304 | 346 | 396 | 442 | 658 | 569 | 691 | 441 | 538 | 166 | 168 | 172 | 253 | 512 | 260 | 126 | 172 | 313 | 143 | 332 | 307 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 8.7 | 7.8 | 7.5 | 7.8 | 8.7 | 7.4 | 7.3 | 7.4 | – | 7.4 | 7.4 | 7.5 | 9.2 | 9.2 | 9.2 | 7.6 | 8.6 | 11.5 | 9.6 | 9.5 | 9.8 |
Std Deviation (1e-4) | 1.3 | 1.7 | 1.6 | 1.8 | 1.8 | 1.6 | 1.6 | 1.5 | – | 1.6 | 1.7 | 1.8 | 1.7 | 1.7 | 1.7 | 2.1 | 2.4 | 3.3 | 1.8 | 1.7 | 1.9 |
Intensity Change (Log2) | – | – | – | 0.06 | 0.22 | -0.02 | -0.04 | -0.02 | – | -0.03 | -0.02 | 0.0 | 0.29 | 0.29 | 0.29 | 0.03 | 0.19 | 0.62 | 0.35 | 0.33 | 0.38 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | – | 0 | 3.2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 5.1 | 6.9 | 8.1 | 8.8 | 7.4 | – | 11.0 | 11.1 | 9.6 | -1.3 | -1.3 | 1.4 | 0.5 | -1.2 | -1.0 |
Endoplasmic Reticulum | -0.7 | -1.2 | 0.5 | -1.4 | 0 | – | -2.1 | -3.3 | 0.2 | 2.1 | 3.1 | 1.1 | -3.1 | -4.6 | -4.2 |
Endosome | -0.1 | -2.4 | -3.2 | -2.1 | -2.8 | – | -3.4 | -3.3 | -4.1 | -2.4 | -2.4 | -2.1 | -1.5 | -1.1 | -2.4 |
Golgi | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 0 | 0 | 0 | – | 0 | 0.7 | 0 | 0 | 0 | 0 | 0 | 1.1 | 0 |
Nucleus | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 3.3 | 2.6 | 2.7 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | -4.3 | -7.0 | -10.9 | -9.1 | -10.2 | – | -12.6 | -11.2 | -12.0 | -0.3 | -0.8 | -3.3 | 2.1 | 3.7 | 3.4 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 5.6103 | 7.6142 | 7.7255 | 6.7073 | 7.0938 | 6.8731 | 3.6637 | 5.6759 | 7.0695 | 6.9155 | 5.2039 | 5.4477 | 3.2792 | 6.18 | 6.1496 | 6.3486 | 4.3191 | 4.7784 |
Actin | 0.0222 | 0.0001 | 0.013 | 0.075 | 0.0018 | 0.0011 | 0.0308 | 0 | 0.0029 | 0.0001 | 0.0247 | 0.0002 | 0.0232 | 0.0001 | 0.0005 | 0 | 0.0221 | 0.0007 |
Bud | 0.0006 | 0.0003 | 0.0046 | 0.0011 | 0.0003 | 0.0003 | 0.0011 | 0.0004 | 0.0005 | 0.0008 | 0.0016 | 0.0007 | 0.0005 | 0.0002 | 0.0003 | 0.0005 | 0.0009 | 0.0003 |
Bud Neck | 0.0021 | 0.0009 | 0.0011 | 0.0008 | 0.0004 | 0.001 | 0.0006 | 0.0012 | 0.0008 | 0.0004 | 0.0027 | 0.0008 | 0.0006 | 0.0004 | 0.0007 | 0.0003 | 0.0008 | 0.0014 |
Bud Periphery | 0.0015 | 0.0006 | 0.0031 | 0.002 | 0.0009 | 0.0008 | 0.0009 | 0.0008 | 0.0008 | 0.0021 | 0.0027 | 0.0025 | 0.0009 | 0.0006 | 0.0008 | 0.0015 | 0.0013 | 0.0011 |
Bud Site | 0.0022 | 0.0016 | 0.0032 | 0.0034 | 0.0005 | 0.0004 | 0.0577 | 0.0049 | 0.0012 | 0.0006 | 0.0058 | 0.0007 | 0.0028 | 0.0007 | 0.0011 | 0.0002 | 0.0119 | 0.0005 |
Cell Periphery | 0.0021 | 0.0031 | 0.002 | 0.0013 | 0.0012 | 0.0014 | 0.0017 | 0.0065 | 0.0049 | 0.002 | 0.0034 | 0.002 | 0.0031 | 0.0017 | 0.0023 | 0.001 | 0.0005 | 0.0017 |
Cytoplasm | 0.0324 | 0.0908 | 0.075 | 0.0209 | 0.0309 | 0.0351 | 0.0373 | 0.0676 | 0.0658 | 0.0175 | 0.0111 | 0.0434 | 0.0429 | 0.1067 | 0.0657 | 0.0169 | 0.0577 | 0.0372 |
Cytoplasmic Foci | 0.0202 | 0.0037 | 0.0049 | 0.0081 | 0.0118 | 0.0043 | 0.0085 | 0.0251 | 0.015 | 0.0008 | 0.017 | 0.0023 | 0.0111 | 0.0023 | 0.0083 | 0.0009 | 0.0132 | 0.0026 |
Eisosomes | 0.0001 | 0 | 0.0002 | 0.0002 | 0 | 0 | 0.0001 | 0 | 0 | 0 | 0.0001 | 0 | 0.0001 | 0 | 0 | 0 | 0.0004 | 0 |
Endoplasmic Reticulum | 0.0984 | 0.1408 | 0.1821 | 0.1124 | 0.0774 | 0.0744 | 0.1054 | 0.1992 | 0.2857 | 0.2353 | 0.0867 | 0.1137 | 0.0569 | 0.1545 | 0.276 | 0.1086 | 0.0453 | 0.0763 |
Endosome | 0.123 | 0.0551 | 0.0834 | 0.1121 | 0.1303 | 0.0744 | 0.1391 | 0.0797 | 0.13 | 0.0619 | 0.2027 | 0.1097 | 0.1432 | 0.0419 | 0.1053 | 0.0701 | 0.1175 | 0.0802 |
Golgi | 0.0122 | 0.0007 | 0.0029 | 0.0079 | 0.0065 | 0.0017 | 0.0026 | 0.0013 | 0.0057 | 0.0006 | 0.031 | 0.002 | 0.008 | 0.0004 | 0.0089 | 0.0007 | 0.0075 | 0.0018 |
Lipid Particles | 0.0114 | 0.0018 | 0.0042 | 0.0027 | 0.0323 | 0.0027 | 0.0102 | 0.0161 | 0.0185 | 0.0017 | 0.0327 | 0.002 | 0.0084 | 0.0038 | 0.0122 | 0.0007 | 0.0142 | 0.0051 |
Mitochondria | 0.0071 | 0.0008 | 0.0042 | 0.0084 | 0.0033 | 0.0012 | 0.0009 | 0.0011 | 0.0036 | 0.0008 | 0.0664 | 0.005 | 0.0059 | 0.0004 | 0.0048 | 0.0009 | 0.0082 | 0.0015 |
None | 0.0032 | 0.0009 | 0.0018 | 0.0006 | 0.0049 | 0.0004 | 0.0009 | 0.0003 | 0.0005 | 0.0001 | 0.0002 | 0.0005 | 0.0053 | 0.0204 | 0.0004 | 0.0001 | 0.0044 | 0.0019 |
Nuclear Periphery | 0.0718 | 0.0519 | 0.0613 | 0.0573 | 0.0688 | 0.0682 | 0.0442 | 0.072 | 0.0706 | 0.054 | 0.0285 | 0.067 | 0.0863 | 0.0818 | 0.0657 | 0.0642 | 0.0452 | 0.0858 |
Nucleolus | 0.0015 | 0.0056 | 0.0006 | 0.0004 | 0.0004 | 0.0007 | 0.0009 | 0.0131 | 0.0003 | 0.0002 | 0.0023 | 0.0004 | 0.002 | 0.0005 | 0.0004 | 0.0001 | 0.0054 | 0.0008 |
Nucleus | 0.0217 | 0.0109 | 0.0094 | 0.0049 | 0.0109 | 0.0183 | 0.0075 | 0.0126 | 0.0112 | 0.0043 | 0.0036 | 0.0065 | 0.021 | 0.0092 | 0.0055 | 0.0043 | 0.0272 | 0.0092 |
Peroxisomes | 0.0042 | 0.0001 | 0.0013 | 0.0095 | 0.0057 | 0.0025 | 0.0011 | 0.0014 | 0.023 | 0 | 0.0055 | 0 | 0.0046 | 0 | 0.0006 | 0 | 0.0051 | 0.0009 |
Punctate Nuclear | 0.0113 | 0.0003 | 0.0012 | 0.0009 | 0.0024 | 0.0014 | 0.0007 | 0.0004 | 0.0009 | 0.0001 | 0.0007 | 0.0002 | 0.0055 | 0.0003 | 0.0005 | 0 | 0.018 | 0.0043 |
Vacuole | 0.4851 | 0.5744 | 0.4831 | 0.5108 | 0.5051 | 0.595 | 0.4673 | 0.4535 | 0.3215 | 0.5281 | 0.365 | 0.5269 | 0.4749 | 0.5046 | 0.3931 | 0.6182 | 0.4592 | 0.5332 |
Vacuole Periphery | 0.0656 | 0.0557 | 0.0573 | 0.0593 | 0.104 | 0.1148 | 0.0804 | 0.0428 | 0.0367 | 0.0886 | 0.1056 | 0.1136 | 0.0928 | 0.0695 | 0.0469 | 0.1109 | 0.1339 | 0.1536 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 61.167 | 26.8037 | 12.1989 | 22.3763 | 34.9878 | 38.358 | 25.7461 | 18.1695 | 17.5047 | 49.2255 |
Translational Efficiency | 0.9123 | 0.7934 | 0.8936 | 0.5837 | 0.8116 | 1.4613 | 0.8894 | 0.6833 | 0.8325 | 0.8519 |
Dataset | Hit |
---|---|
Protein Concentration | ✔ |
Protein Localization | ✘ |
Gene Expression | ✔ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
962 | 825 | 221 | 60 | 1312 | 1708 | 1974 | 1534 | 2274 | 2533 | 2195 | 1594 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 887.61 | 941.61 | 1125.55 | 1145.98 | 733.98 | 864.78 | 1021.87 | 1093.48 | 798.97 | 889.80 | 1032.31 | 1095.46 |
Standard Deviation | 132.56 | 168.12 | 133.09 | 142.97 | 141.12 | 134.44 | 149.23 | 158.29 | 157.12 | 150.63 | 150.95 | 158.06 |
Intensity Change Log 2 | — | 0.085204 | 0.342632 | 0.368584 | — | 0.236592 | 0.477399 | 0.575114 | — | 0.155700 | 0.405195 | 0.465742 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.000209 | 0.000060 | 0.000174 | 0.000231 | 0.000493 | 0.000063 | 0.000076 | 0.000059 | 0.000373 | 0.000062 | 0.000086 | 0.000065 |
Bud Neck | 0.004247 | 0.002580 | 0.003024 | 0.005608 | 0.006567 | 0.001317 | 0.002058 | 0.004500 | 0.005585 | 0.001728 | 0.002155 | 0.004542 |
Bud Site | 0.012200 | 0.005304 | 0.004278 | 0.012108 | 0.011811 | 0.003219 | 0.003686 | 0.008929 | 0.011975 | 0.003898 | 0.003746 | 0.009048 |
Cell Periphery | 0.000505 | 0.000273 | 0.000117 | 0.000286 | 0.001313 | 0.000288 | 0.000262 | 0.000182 | 0.000971 | 0.000283 | 0.000247 | 0.000186 |
Cytoplasm | 0.104796* | 0.124675* | 0.159953* | 0.056813 | 0.142482* | 0.060798 | 0.081340 | 0.073101 | 0.126540* | 0.081603 | 0.089255 | 0.072488 |
Cytoplasmic Foci | 0.043293 | 0.045020 | 0.039362 | 0.031120 | 0.061103 | 0.033140 | 0.016647 | 0.016674 | 0.053568 | 0.037009 | 0.018934 | 0.017218 |
Eisosomes | 0.000033 | 0.000013 | 0.000026 | 0.000034 | 0.000088 | 0.000015 | 0.000007 | 0.000007 | 0.000065 | 0.000014 | 0.000009 | 0.000008 |
Endoplasmic Reticulum | 0.156770* | 0.116240* | 0.053925 | 0.061430 | 0.194175* | 0.207315* | 0.258995* | 0.110478* | 0.178351* | 0.177652* | 0.238348* | 0.108632* |
Endosome | 0.071675 | 0.045492 | 0.058484 | 0.061716 | 0.052481 | 0.045304 | 0.042851 | 0.044567 | 0.060601 | 0.045365 | 0.044425 | 0.045213 |
Golgi | 0.003154 | 0.001214 | 0.000723 | 0.001454 | 0.005530 | 0.003096 | 0.002070 | 0.002546 | 0.004525 | 0.002483 | 0.001934 | 0.002505 |
Lipid Particles | 0.014276 | 0.007531 | 0.009502 | 0.009977 | 0.016398 | 0.009236 | 0.007118 | 0.005437 | 0.015501 | 0.008681 | 0.007358 | 0.005608 |
Mitochondria | 0.004102 | 0.000437 | 0.000163 | 0.000077 | 0.002582 | 0.003733 | 0.000842 | 0.002940 | 0.003225 | 0.002659 | 0.000774 | 0.002832 |
Mitotic Spindle | 0.005731 | 0.000411 | 0.001372 | 0.004629 | 0.000124 | 0.000506 | 0.000550 | 0.004551 | 0.002496 | 0.000475 | 0.000633 | 0.004554 |
None | 0.000196 | 0.000755 | 0.000167 | 0.000068 | 0.003025 | 0.001124 | 0.000272 | 0.000566 | 0.001828 | 0.001004 | 0.000261 | 0.000548 |
Nuclear Periphery | 0.018371 | 0.003399 | 0.015847 | 0.006239 | 0.001377 | 0.003444 | 0.012521 | 0.011547 | 0.008567 | 0.003429 | 0.012856 | 0.011348 |
Nuclear Periphery Foci | 0.018135 | 0.006464 | 0.047478 | 0.026613 | 0.002059 | 0.003164 | 0.023899 | 0.008177 | 0.008860 | 0.004239 | 0.026273 | 0.008871 |
Nucleolus | 0.001254 | 0.000962 | 0.000962 | 0.001516 | 0.001019 | 0.000615 | 0.000552 | 0.001101 | 0.001118 | 0.000728 | 0.000593 | 0.001117 |
Nucleus | 0.017359 | 0.009521 | 0.028381 | 0.023127 | 0.009318 | 0.006051 | 0.005596 | 0.008654 | 0.012720 | 0.007181 | 0.007890 | 0.009199 |
Peroxisomes | 0.001394 | 0.000438 | 0.000503 | 0.001931 | 0.001116 | 0.000821 | 0.000155 | 0.000229 | 0.001234 | 0.000697 | 0.000190 | 0.000293 |
Vacuole | 0.509616* | 0.588519* | 0.559043* | 0.686123* | 0.453937* | 0.558460* | 0.509863* | 0.652489* | 0.477492* | 0.568250* | 0.514814* | 0.653755* |
Vacuole Periphery | 0.012684 | 0.040691 | 0.016519 | 0.008898 | 0.033002 | 0.058291 | 0.030639 | 0.043266 | 0.024407 | 0.052559 | 0.029217 | 0.041972 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | 3.22 | 0.60 | -0.80 | -3.81 | -1.46 | 1.52 | 1.46 | 1.52 | 0.03 | 1.10 | 1.89 | 1.73 | 1.85 | -0.46 | 1.28 |
Bud Neck | 2.25 | 2.07 | -1.03 | -2.45 | -2.23 | 7.06 | 5.50 | 1.92 | -6.92 | -4.56 | 7.52 | 6.01 | 1.10 | -6.05 | -4.68 |
Bud Site | 3.03 | 3.61 | -0.10 | -1.80 | -2.07 | 6.18 | 5.90 | 1.47 | -4.94 | -4.61 | 6.51 | 6.69 | 1.64 | -4.68 | -4.84 |
Cell Periphery | 0.63 | 1.16 | 0.68 | 0.28 | -2.04 | 7.58 | 7.68 | 8.46 | 4.47 | 3.29 | 4.31 | 4.51 | 5.00 | 4.92 | 2.85 |
Cytoplasm | -2.24 | -3.97 | 2.88 | 4.08 | 5.23 | 13.30 | 9.15 | 9.81 | -3.55 | 0.99 | 9.03 | 6.55 | 9.19 | 0.94 | 2.79 |
Cytoplasmic Foci | -0.46 | 1.23 | 1.84 | 2.03 | 1.19 | 8.45 | 14.67 | 14.66 | 8.57 | -0.09 | 6.91 | 16.25 | 16.95 | 11.52 | 1.25 |
Eisosomes | 5.95 | 1.37 | -0.09 | -3.42 | -1.05 | 7.25 | 7.96 | 7.99 | 7.10 | 0.52 | 8.39 | 9.20 | 9.42 | 6.68 | 1.61 |
Endoplasmic Reticulum | 3.44 | 9.77 | 5.69 | 3.61 | 0.02 | -1.35 | -6.21 | 9.60 | 12.35 | 18.15 | -0.12 | -7.25 | 9.77 | 10.32 | 16.83 |
Endosome | 6.42 | 2.37 | 1.18 | -0.79 | 0.11 | 2.63 | 3.78 | 3.05 | 0.53 | -0.58 | 6.51 | 7.10 | 6.28 | 0.39 | -0.13 |
Golgi | 2.19 | 2.92 | 1.69 | -0.07 | -0.78 | 2.95 | 5.15 | 3.92 | 0.84 | -0.77 | 3.22 | 4.66 | 3.04 | 0.07 | -0.98 |
Lipid Particles | 4.64 | 3.24 | 0.80 | -1.12 | -0.59 | 5.12 | 7.18 | 8.95 | 4.62 | 2.90 | 6.70 | 8.59 | 11.16 | 4.42 | 3.02 |
Mitochondria | 2.40 | 2.59 | 2.65 | 5.87 | 3.07 | -1.45 | 6.59 | -0.61 | 0.99 | -4.76 | 0.62 | 3.72 | 0.53 | -0.17 | -4.87 |
Mitotic Spindle | 2.94 | 2.41 | -0.48 | -1.56 | -1.38 | -1.80 | -3.44 | -4.98 | -4.51 | -4.49 | 2.60 | 2.41 | -2.23 | -4.76 | -4.62 |
None | -2.03 | 0.11 | 2.64 | 2.45 | 1.77 | 1.85 | 2.88 | 2.52 | 1.24 | -1.33 | 1.34 | 2.82 | 2.19 | 1.32 | -1.34 |
Nuclear Periphery | 7.29 | 0.71 | 4.82 | -1.13 | 2.67 | -4.53 | -11.41 | -9.24 | -7.60 | 0.71 | 5.57 | -3.58 | -2.19 | -7.65 | 1.13 |
Nuclear Periphery Foci | 6.36 | -5.72 | -1.21 | -3.10 | 2.75 | -2.33 | -13.75 | -6.77 | -5.61 | 8.68 | 5.73 | -10.54 | -0.21 | -5.11 | 9.95 |
Nucleolus | 0.77 | 0.80 | -0.05 | -0.39 | -0.40 | 2.04 | 2.43 | -0.34 | -4.98 | -6.60 | 2.05 | 2.90 | 0.05 | -3.59 | -5.97 |
Nucleus | 3.81 | -2.59 | -0.27 | -0.79 | 0.56 | 2.54 | 2.82 | 0.16 | -3.57 | -4.21 | 4.95 | 3.91 | 2.38 | -2.37 | -1.31 |
Peroxisomes | 1.95 | 1.83 | -0.26 | -1.23 | -1.17 | 1.06 | 9.27 | 8.47 | 2.44 | -3.24 | 1.95 | 4.81 | 4.29 | 2.41 | -2.18 |
Vacuole | -5.28 | -1.92 | -5.28 | -3.13 | -3.83 | -9.19 | -5.04 | -17.31 | -9.15 | -14.30 | -10.11 | -3.87 | -18.17 | -9.29 | -14.66 |
Vacuole Periphery | -10.81 | -1.36 | 2.00 | 10.35 | 2.69 | -10.02 | 1.42 | -3.87 | 5.99 | -5.53 | -15.08 | -3.08 | -8.21 | 4.97 | -5.88 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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