Standard name
Human Ortholog
Description Protein of unknown function; involved in maintenance of proper telomere length; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus; forms nuclear foci upon DNA replication stress
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Cytoplasm 0.4 0.48 0.57 0.47 0.3 0.42 0.34 – 0.23 0.28 0.19 0.19 0.62 0.57 0.62 – 0.29 0.4 0.27 0.3 0.29 0.28 0.25 0.36
Endoplasmic Reticulum 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – 0.07 0.06 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – 0.05 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Mitochondria 0.05 0.14 0 0.22 0.25 0.28 0.26 – 0.71 0.6 0.7 0.67 0 0 0 – 0.06 0.05 0 0 0.07 0 0.05 0.07
Nucleus 0.3 0.28 0.27 0.36 0.5 0.3 0.41 – 0.35 0.33 0.34 0.4 0.31 0.34 0.25 – 0.36 0.4 0.36 0.33 0.27 0.23 0.19 0.09
Nuclear Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – 0 0.05 0 0 0 0.05 0.07 0.05
Vac/Vac Membrane 0.35 0.28 0.13 0.06 0 0.14 0.09 – 0 0.05 0.09 0.07 0.1 0.1 0.17 – 0.31 0.16 0.25 0.24 0.27 0.3 0.27 0.3
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – 0 0 0 0 0 2 2 2
Bud 0 1 0 0 0 0 0 – 3 1 2 3 0 0 0 – 0 1 3 2 2 6 13 9
Bud Neck 0 0 3 1 0 0 2 – 1 3 3 2 0 4 1 – 0 0 1 1 1 3 4 5
Bud Site 0 0 0 0 0 1 0 – 2 5 6 1 0 0 0 – 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 1 0 – 0 1 0 0 0 0 0 0
Cytoplasm 105 111 85 45 6 84 45 – 41 48 38 28 122 186 216 – 72 127 87 83 56 76 94 156
Endoplasmic Reticulum 0 0 0 2 0 0 1 – 3 0 0 2 1 1 1 – 17 19 0 1 1 0 3 5
Endosome 1 1 3 0 0 3 3 – 0 0 0 0 2 1 3 – 12 7 3 6 1 0 8 7
Golgi 1 0 0 0 0 0 0 – 0 0 0 0 1 0 0 – 1 1 1 1 0 0 0 0
Mitochondria 13 32 6 21 5 55 34 – 127 101 142 101 0 5 1 – 15 15 9 8 14 9 19 31
Nucleus 79 65 40 35 10 59 54 – 63 56 70 60 61 110 89 – 89 127 117 94 52 61 73 39
Nuclear Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – 0 1 0 0 1 0 1 2
Nucleolus 0 1 0 0 0 0 0 – 0 3 0 0 0 0 0 – 0 1 0 0 0 4 8 2
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 1
SpindlePole 0 1 2 0 0 1 1 – 1 0 0 0 0 3 3 – 2 15 11 9 3 14 25 22
Vac/Vac Membrane 91 66 19 6 0 28 12 – 5 8 18 10 19 31 60 – 78 52 81 66 52 79 104 129
Unique Cell Count 261 233 150 96 20 199 132 180 169 204 151 196 324 351 250 319 325 282 194 270 381 439
Labelled Cell Count 290 278 158 110 21 231 152 246 225 279 207 206 342 374 286 367 325 282 194 270 381 439


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.2 3.8 5.0 4.4 3.7 4.0 4.3 – 3.3 3.5 3.4 3.4 5.5 5.5 5.2 4.2 5.3 5.2 6.4 6.7 6.4
Std Deviation (1e-4) 0.6 0.7 1.6 1.4 0.7 1.3 1.2 – 0.7 0.7 1.0 0.6 1.6 1.4 1.1 1.0 1.6 1.1 2.1 2.3 1.7
Intensity Change (Log2) – – – -0.2 -0.42 -0.32 -0.22 – -0.61 -0.53 -0.56 -0.54 0.13 0.14 0.05 -0.26 0.09 0.05 0.35 0.43 0.36


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – 0 0
Bud Neck – – – – – – – – – – – – – 0 0
Bud Site – – – – – – – – – – – – – 0 0
Cell Periphery – – – – – – – – – – – – – 0 0
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – 0 0
Endosome – – – – – – – – – – – – – 0 0
Golgi – – – – – – – – – – – – – 0 0
Mitochondria – – – – – – – – – – – – – 0 0
Nuclear Periphery – – – – – – – – – – – – – 0 0
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – 0 0
Peroxisomes – – – – – – – – – – – – – 0 0
SpindlePole – – – – – – – – – – – – – 0 0
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – 0 0
Cytoplasm – – – – – – – – – – – – – 0 0
Nucleus – – – – – – – – – – – – – 0 0
Vacuole – – – – – – – – – – – – – 4.2 1.0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration -1.1064 -0.6073 -0.8955 -0.9159 -1.1527 -0.7519 4.6325 4.9135 3.9733 4.0951 3.7183 4.1386 1.8939 1.5421 1.4739 3.0323 1.908 1.3219
Actin 0.0137 0.0002 0.0019 0.0007 0.0001 0.0007 0.0307 0.0009 0.0018 0.0271 0.0382 0.0033 0.0592 0.0007 0.0076 0.0996 0.0369 0.0002
Bud 0.0002 0.0001 0.0002 0 0 0.0001 0.0046 0.003 0.0016 0.0031 0.0002 0.0007 0.0012 0.0003 0.0003 0.0059 0.0002 0
Bud Neck 0.0008 0.0004 0.0009 0.0009 0.0003 0.0015 0.0164 0.0018 0.004 0.0014 0.0023 0.0029 0.0022 0.0004 0.0015 0.0012 0.0028 0.001
Bud Periphery 0.0003 0.0001 0.0002 0 0 0.0002 0.0054 0.0081 0.0015 0.0132 0.0006 0.0035 0.0012 0.0005 0.0004 0.0073 0.0002 0
Bud Site 0.0005 0.0015 0.0006 0.0001 0 0.0002 0.0068 0.0121 0.0055 0.0031 0.0003 0.0002 0.0075 0.001 0.008 0.0164 0.0014 0.0001
Cell Periphery 0.0001 0.0001 0.0001 0.0001 0 0 0.0004 0.0002 0.0008 0.0004 0.0002 0.0002 0.0007 0.0001 0.0001 0.0003 0.0001 0
Cytoplasm 0.1016 0.1277 0.0756 0.0428 0.0435 0.136 0.1252 0.1951 0.1376 0.0383 0.1338 0.1926 0.0775 0.1321 0.0756 0.0339 0.1012 0.1381
Cytoplasmic Foci 0.0125 0.0033 0.0144 0.0019 0.0053 0.0093 0.0189 0.0075 0.0101 0.0071 0.0234 0.0064 0.0186 0.008 0.0102 0.0415 0.0402 0.0026
Eisosomes 0.0003 0.0003 0.0002 0.0003 0.0001 0.0001 0.0003 0.0001 0.0004 0.0006 0.0007 0.0001 0.0003 0.0001 0.0003 0.0006 0.0002 0.0001
Endoplasmic Reticulum 0.0027 0.0016 0.0019 0.0062 0.0005 0.0013 0.0058 0.0026 0.003 0.0034 0.0022 0.002 0.0029 0.0036 0.0015 0.0052 0.0036 0.0017
Endosome 0.005 0.0018 0.0045 0.0019 0.0008 0.0047 0.0187 0.004 0.0042 0.0108 0.0031 0.0021 0.0147 0.0085 0.0095 0.0752 0.0208 0.0017
Golgi 0.0008 0.0001 0.0007 0.0001 0 0.0007 0.0036 0.0003 0.0018 0.0057 0.0007 0.0001 0.0118 0.0014 0.0059 0.0375 0.0201 0
Lipid Particles 0.0028 0.0011 0.0021 0.0005 0.0001 0.0022 0.0062 0.0004 0.0019 0.0081 0.0011 0.0003 0.0278 0.0019 0.0075 0.0214 0.0273 0.0002
Mitochondria 0.0006 0.0005 0.0013 0.0005 0.0002 0.004 0.0049 0.0013 0.0052 0.0077 0.0004 0.0005 0.0107 0.0007 0.0262 0.0332 0.0042 0.0003
None 0.5867 0.6468 0.6031 0.5182 0.792 0.5251 0.4755 0.5261 0.5918 0.6328 0.6213 0.5336 0.5784 0.6644 0.7133 0.4855 0.6795 0.6753
Nuclear Periphery 0.0158 0.0087 0.0181 0.0526 0.002 0.0063 0.0309 0.0086 0.0088 0.0056 0.0019 0.0048 0.0233 0.0054 0.0029 0.0087 0.0021 0.0048
Nucleolus 0.0033 0.0032 0.0052 0.003 0.0016 0.0039 0.0034 0.0034 0.0024 0.0023 0.0008 0.0029 0.0056 0.0042 0.0012 0.0006 0.0008 0.0022
Nucleus 0.2194 0.1767 0.1984 0.3554 0.146 0.2641 0.2025 0.1684 0.1919 0.2111 0.1607 0.2071 0.1241 0.1566 0.1155 0.0929 0.0034 0.1608
Peroxisomes 0.0009 0.0001 0.0009 0.0001 0.0001 0.0026 0.0044 0.0054 0.0045 0.0081 0.0004 0.0003 0.0157 0.0008 0.0056 0.0221 0.0168 0.0001
Punctate Nuclear 0.0296 0.0241 0.0677 0.0132 0.0068 0.0359 0.0253 0.048 0.0181 0.0062 0.0069 0.0347 0.0085 0.0064 0.0046 0.0074 0.0367 0.01
Vacuole 0.002 0.0014 0.0013 0.0009 0.0004 0.0008 0.0072 0.0025 0.0025 0.0028 0.0007 0.0015 0.006 0.0026 0.0019 0.0021 0.001 0.0006
Vacuole Periphery 0.0004 0.0003 0.0006 0.0007 0.0001 0.0002 0.0029 0.0004 0.0005 0.0012 0.0001 0.0003 0.0022 0.0004 0.0003 0.0014 0.0003 0.0002

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 18.931 8.4616 8.9698 17.0078 17.532 5.4059 13.4443 15.2608 15.7168 19.6261
Translational Efficiency 0.5511 0.79 0.8196 0.4101 0.351 2.2357 0.5404 0.4876 0.5053 0.537

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
127 395 2305 2075 1420 2028 2163 1481 1547 2423 4468 3556

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 659.84 760.05 778.27 832.52 657.79 795.33 1023.34 777.80 657.96 789.58 896.91 809.73
Standard Deviation 70.33 89.13 97.31 125.42 77.21 92.20 1049.08 115.98 76.67 92.63 743.42 124.54
Intensity Change Log 2 — 0.203978 0.238155 0.335369 — 0.273927 0.637587 0.241772 — 0.239322 0.451341 0.289402

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000047 0.000970 0.000186 0.002847 0.000029 0.000565 0.000407 0.000362 0.000030 0.000631 0.000293 0.001812
Bud Neck 0.014920 0.016636 0.008759 0.020695 0.001949 0.025897 0.008824 0.007807 0.003013 0.024387 0.008790 0.015328
Bud Site 0.003466 0.013503 0.004782 0.043281 0.000419 0.009383 0.011590 0.019704 0.000670 0.010054 0.008078 0.033461
Cell Periphery 0.000171 0.000226 0.000123 0.000720 0.000118 0.000243 0.001918 0.000293 0.000123 0.000240 0.000992 0.000542
Cytoplasm 0.093663 0.230046* 0.102061* 0.305854* 0.299070* 0.315174* 0.147051* 0.281371* 0.282208* 0.301296* 0.123841* 0.295657*
Cytoplasmic Foci 0.025579 0.086896 0.000722 0.011639 0.039760 0.089314 0.003973 0.009893 0.038596 0.088919 0.002296 0.010912
Eisosomes 0.000061 0.000100 0.000011 0.000039 0.000035 0.000082 0.000019 0.000035 0.000037 0.000085 0.000015 0.000037
Endoplasmic Reticulum 0.001482 0.004131 0.002414 0.002609 0.000625 0.000857 0.002537 0.002876 0.000696 0.001391 0.002473 0.002721
Endosome 0.003333 0.009748 0.000428 0.007852 0.000899 0.007984 0.000794 0.003228 0.001098 0.008271 0.000605 0.005926
Golgi 0.000190 0.002942 0.000047 0.006933 0.000157 0.002127 0.002670 0.002219 0.000160 0.002260 0.001317 0.004970
Lipid Particles 0.006464 0.006183 0.000179 0.000956 0.002593 0.005478 0.001300 0.001141 0.002911 0.005593 0.000722 0.001033
Mitochondria 0.002419 0.004875 0.001133 0.010110 0.001142 0.008016 0.002311 0.002413 0.001247 0.007504 0.001704 0.006904
Mitotic Spindle 0.000119 0.006281 0.002447 0.095421 0.000446 0.005917 0.007588 0.018388 0.000419 0.005976 0.004936 0.063339
None 0.008942 0.005873 0.004437 0.007803 0.010561 0.006925 0.007491 0.016391 0.010428 0.006754 0.005915 0.011380
Nuclear Periphery 0.001052 0.001526 0.001421 0.003293 0.000849 0.001038 0.001971 0.002115 0.000866 0.001118 0.001688 0.002802
Nuclear Periphery Foci 0.000200 0.001243 0.000159 0.001305 0.000317 0.000290 0.001640 0.001276 0.000307 0.000445 0.000876 0.001293
Nucleolus 0.008715 0.003884 0.000415 0.000989 0.001689 0.002694 0.000932 0.001483 0.002266 0.002888 0.000666 0.001195
Nucleus 0.817002* 0.554329* 0.863560* 0.412278* 0.633380* 0.485387* 0.766857* 0.593510* 0.648455* 0.496626* 0.816745* 0.487757*
Peroxisomes 0.002017 0.014081 0.000078 0.002051 0.000832 0.007697 0.000135 0.001921 0.000929 0.008738 0.000106 0.001997
Vacuole 0.009639 0.034997 0.006429 0.058017 0.004925 0.023777 0.024105 0.032679 0.005312 0.025606 0.014986 0.047464
Vacuole Periphery 0.000520 0.001531 0.000210 0.005308 0.000204 0.001156 0.005888 0.000895 0.000230 0.001217 0.002958 0.003470

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -3.71 -6.28 -2.91 -0.50 -2.48 -2.35 -15.54 -11.28 0.53 0.46 -3.11 -16.37 -3.65 -0.90 -2.37
Bud Neck -0.24 0.72 -0.18 0.22 -4.88 -12.77 -10.58 -8.80 8.86 1.53 -12.30 -7.42 -10.52 6.63 -3.71
Bud Site -3.55 -0.74 -8.35 -2.67 -9.14 -8.42 -10.62 -7.82 -2.70 -1.91 -9.52 -10.97 -14.37 -5.70 -8.02
Cell Periphery -1.18 1.46 -1.08 -0.63 -1.59 -3.57 -5.50 -3.66 0.55 5.08 -3.94 -5.42 -2.10 -0.38 3.73
Cytoplasm -6.80 -0.03 -9.38 -1.24 -22.50 -1.63 20.32 6.88 9.02 -12.40 -1.97 23.54 5.83 9.09 -24.40
Cytoplasmic Foci -6.96 4.55 3.66 12.29 -9.73 -12.46 16.24 13.74 24.19 -5.20 -13.91 17.69 15.23 27.69 -9.65
Eisosomes -3.37 6.54 2.21 5.71 -12.71 -6.12 5.90 -4.65 4.67 -11.39 -7.24 10.79 -3.62 6.02 -16.16
Endoplasmic Reticulum -4.43 -3.16 -5.07 0.76 -4.81 -2.29 -16.33 -15.46 -14.87 -2.40 -5.82 -19.89 -20.80 -15.62 -4.95
Endosome -2.95 2.57 0.26 3.57 -7.63 -9.75 0.75 -4.02 6.46 -4.24 -10.44 3.69 -6.13 7.37 -8.47
Golgi -2.52 1.83 -3.51 0.57 -3.86 -6.16 -6.17 -1.68 1.28 0.99 -6.56 -5.37 -3.83 0.94 -1.87
Lipid Particles 0.26 3.84 3.38 5.27 -13.66 -5.97 5.20 5.57 10.55 -0.29 -5.65 8.30 6.94 12.10 -4.66
Mitochondria -1.95 1.51 -2.52 -0.25 -6.31 -8.93 -2.75 -1.36 7.73 0.84 -9.21 -1.24 -4.76 5.36 -5.01
Mitotic Spindle -3.28 -4.44 -14.58 -10.83 -13.56 -5.21 -6.11 -7.09 -4.62 -3.58 -6.00 -6.95 -15.94 -12.81 -13.36
None 1.16 1.74 0.81 -1.08 -4.52 3.04 3.88 -0.85 -3.26 -3.79 3.38 5.47 1.37 -2.44 -5.23
Nuclear Periphery -1.78 -3.81 -15.94 -10.44 -16.28 -1.21 -8.31 -7.19 -6.82 -0.57 -1.83 -8.87 -17.06 -16.85 -11.17
Nuclear Periphery Foci -3.03 -0.73 -5.50 1.84 -5.21 -0.06 -4.78 -2.87 -2.87 -0.60 -2.22 -3.86 -4.32 -2.68 -1.97
Nucleolus 1.71 3.08 2.73 4.25 -4.78 -3.55 2.50 -0.76 1.52 -1.93 -2.20 5.13 1.60 4.00 -3.74
Nucleus 9.37 -2.32 12.09 1.47 41.93 13.92 -14.67 1.06 -12.22 15.08 15.25 -21.06 9.24 -7.78 39.44
Peroxisomes -5.43 3.55 2.24 6.22 -2.75 -9.11 2.93 -0.27 7.31 -1.69 -10.68 3.61 0.38 10.76 -2.83
Vacuole -5.46 0.39 -18.27 -11.79 -25.23 -13.84 -12.42 -17.91 -11.39 -9.84 -15.38 -11.43 -31.29 -21.33 -25.47
Vacuole Periphery -2.81 2.11 -3.12 -0.64 -4.33 -7.09 -6.69 -2.51 2.88 5.89 -7.90 -6.47 -4.68 -0.26 2.41
Standard name
Human Ortholog
Description Protein of unknown function; involved in maintenance of proper telomere length; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus; forms nuclear foci upon DNA replication stress
Localization
Cell Percentages nucleus (28%), cytoplasm (8%), mixed (57%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Mte1

Mte1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Mte1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available