Standard name
Human Ortholog
Description Protein proposed to regulate endocytosis of plasma membrane proteins; regulates by recruiting the ubiquitin ligase Rsp5p to its target in the plasma membrane; SWAT-GFP and mCherry fusion proteins localize to the cytosol
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 0 0 0 – – – 0 0 0 0 0 0 – 0 0
Bud 0 0 0 0 0 0 0 – 0 0 0.05 0 – – – 0 0 0 0 0 0 – 0 0
Bud Neck 0 0 0.06 0 0 0 0 – 0 0 0 0 – – – 0 0 0 0 0 0 – 0 0.05
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 – – – 0 0 0 – – – – – –
Cell Periphery 0 0 0 0.27 0.06 0.06 0 – 0 0.05 0 0 – – – 0 0.07 0.09 0 0 0 – 0 0
Cytoplasm 0.96 0.99 0.88 0.92 0.98 0.96 0.94 – 0.86 0.89 0.73 0.82 – – – 0.95 0.94 0.96 0.91 0.91 0.74 – 0.86 0.67
Endoplasmic Reticulum 0 0 0.12 0 0 0 0 – 0 0 0 0 – – – 0 0 0 0 0 0 – 0 0
Endosome 0 0 0 0 0 0 0 – 0 0 0 0 – – – 0 0 0 0 0 0 – 0 0.08
Golgi 0 0 0 0 0 0 0 – 0 0 0 0 – – – 0 0 0 0 0 0 – 0 0
Mitochondria 0 0 0 0 0 0 0 – 0.2 0.11 0.36 0.19 – – – 0 0 0 0 0 0 – 0 0.05
Nucleus 0 0 0 0 0 0 0 – 0 0 0 0 – – – 0 0 0 0 0 0 – 0 0
Nuclear Periphery 0 0 0 0 0 0 0 – 0 0 0 0 – – – 0 0 0 0 0 0 – 0 0
Nucleolus 0 0 0 0 0 0 0 – 0 0 0 0 – – – 0 0 0 0 0 0 – 0 0
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 – – – 0 0 0 0 0 0 – 0 0
SpindlePole 0 0 0 0 0 0 0 – 0 0 0 0 – – – 0 0 0 0 0 0 – 0 0
Vac/Vac Membrane 0 0 0.06 0 0 0 0 – 0 0 0 0 – – – 0 0 0 0 0 0 – 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 1 0 – 2 2 0 1 – – – 0 1 0 1 0 0 – 0 0
Bud 1 2 0 0 0 0 1 – 2 3 8 1 – – – 0 0 0 0 2 0 – 0 0
Bud Neck 0 1 1 0 0 0 4 – 0 0 0 2 – – – 0 1 0 1 3 1 – 0 0
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 – – – 0 0 0 – – – – – –
Cell Periphery 2 2 0 7 8 11 9 – 4 9 2 6 – – – 8 23 26 0 0 1 – 0 0
Cytoplasm 95 146 14 24 130 173 195 – 114 176 128 175 – – – 326 300 280 96 133 36 – 28 13
Endoplasmic Reticulum 1 1 2 1 2 4 1 – 3 7 1 5 – – – 8 6 7 0 1 0 – 0 0
Endosome 0 0 0 0 0 0 0 – 0 0 2 0 – – – 4 1 1 2 1 1 – 0 1
Golgi 0 0 0 0 0 0 0 – 0 0 0 0 – – – 0 1 1 1 0 1 – 0 0
Mitochondria 4 0 0 1 0 5 6 – 26 21 63 40 – – – 0 5 3 0 0 1 – 0 1
Nucleus 0 0 0 0 0 2 1 – 1 1 2 1 – – – 5 8 6 0 0 0 – 0 0
Nuclear Periphery 0 0 0 0 0 0 0 – 0 0 0 0 – – – 0 0 0 0 0 0 – 0 0
Nucleolus 0 0 0 0 0 0 0 – 1 0 1 0 – – – 0 0 0 0 0 0 – 0 0
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 – – – 0 0 0 0 0 0 – 0 0
SpindlePole 0 0 0 0 0 0 1 – 0 0 0 0 – – – 0 0 1 0 0 0 – 0 0
Vac/Vac Membrane 0 0 1 1 1 1 1 – 0 1 1 3 – – – 13 7 7 0 1 1 – 0 0
Unique Cell Count 99 147 16 26 133 181 207 133 198 176 213 343 320 292 107 148 50 33 20
Labelled Cell Count 103 152 18 34 141 197 219 153 220 208 234 364 353 332 107 148 50 33 20


Ambiguous

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.1 6.9 8.1 8.7 6.4 5.5 5.2 – 4.2 4.8 4.3 4.6 – – – 8.8 8.3 8.0 – 5.5 –
Std Deviation (1e-4) 0.7 1.4 4.4 5.7 1.3 1.5 0.9 – 0.7 0.7 1.3 0.7 – – – 1.5 1.1 1.5 – 0.4 –
Intensity Change (Log2) – – – 0.1 -0.36 -0.57 -0.64 – -0.94 -0.77 -0.91 -0.84 – – – 0.11 0.03 -0.03 – -0.56 –


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 – 0 0 0 0 – – – 0 0 0
Bud 0 0 0 0 – 0 0 0 0 – – – 0 0 0
Bud Neck 0 0 0 0 – 0 0 0 0 – – – 0 0 0
Bud Site 0 0 0 0 – 0 0 0 0 – – – 0 0 0
Cell Periphery 0 0 0 0 – 0 0 0 0 – – – 0 0 0
Cytoplasm 0.5 2.1 1.4 1.1 – -0.2 0.2 -1.3 -0.5 – – – 1.3 1.0 1.6
Endoplasmic Reticulum 0 0 0 0 – 0 0 0 0 – – – 0 0 0
Endosome 0 0 0 0 – 0 0 0 0 – – – 0 0 0
Golgi 0 0 0 0 – 0 0 0 0 – – – 0 0 0
Mitochondria 0 0 0 0 – 0 0 0 0 – – – 0 0 0
Nucleus 0 0 0 0 – 0 0 0 0 – – – 0 0 0
Nuclear Periphery 0 0 0 0 – 0 0 0 0 – – – 0 0 0
Nucleolus 0 0 0 0 – 0 0 0 0 – – – 0 0 0
Peroxisomes 0 0 0 0 – 0 0 0 0 – – – 0 0 0
SpindlePole 0 0 0 0 – 0 0 0 0 – – – 0 0 0
Vacuole 0 0 0 0 – 0 0 0 0 – – – 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 1.4572 2.2353 1.9886 2.0677 1.2941 1.9874 1.7543 2.5535 2.3458 2.3688 1.8478 2.4051 0.6263 1.3976 1.0173 0.8648 1.0851 1.1804
Actin 0.0216 0.0009 0.0171 0.0012 0.0119 0.003 0.0444 0.0025 0.0158 0.0063 0.0608 0.0131 0.019 0.0009 0.0032 0.0071 0.0106 0.0026
Bud 0.0029 0.0002 0.0061 0.0007 0.0142 0.0032 0.0014 0.0002 0.0004 0.0005 0.0027 0.0001 0.0027 0.0002 0.0001 0.0034 0.0006 0.0001
Bud Neck 0.0016 0.0009 0.0035 0.0006 0.0013 0.0055 0.0037 0.0011 0.0016 0.0021 0.0039 0.0033 0.0009 0.0003 0.0004 0.0011 0.0009 0.0016
Bud Periphery 0.0024 0.0004 0.0041 0.0013 0.0092 0.0024 0.0044 0.0004 0.0016 0.0005 0.0085 0.0003 0.0024 0.0002 0.0001 0.0069 0.0008 0.0001
Bud Site 0.0095 0.0111 0.0086 0.0005 0.0291 0.0003 0.0082 0.0205 0.0063 0.0052 0.0108 0.0002 0.0042 0.0044 0.0017 0.004 0.0017 0.0001
Cell Periphery 0.0027 0.0033 0.0037 0.0005 0.0012 0.0008 0.004 0.0026 0.0041 0.001 0.0022 0.0009 0.0019 0.0012 0.0007 0.0009 0.0003 0.0006
Cytoplasm 0.6304 0.797 0.7327 0.773 0.6376 0.7862 0.7019 0.806 0.7617 0.841 0.7321 0.8451 0.7915 0.8785 0.7953 0.7651 0.703 0.8434
Cytoplasmic Foci 0.01 0.0028 0.0041 0.0026 0.0026 0.0057 0.0242 0.0052 0.0101 0.0101 0.007 0.0025 0.0072 0.0019 0.0061 0.0041 0.0125 0.0045
Eisosomes 0.0003 0.0001 0.0003 0.0001 0.0002 0.0001 0.0019 0.0001 0.0002 0.0002 0.0005 0.0001 0.0003 0.0001 0.0001 0.0004 0.0001 0
Endoplasmic Reticulum 0.0043 0.0016 0.0036 0.0013 0.0024 0.0024 0.0056 0.0039 0.0039 0.0062 0.0121 0.0022 0.0044 0.0024 0.004 0.0031 0.0058 0.002
Endosome 0.0049 0.0005 0.0017 0.0004 0.0008 0.0007 0.0104 0.0012 0.0037 0.0172 0.0092 0.0027 0.0082 0.0003 0.0045 0.0077 0.0175 0.0025
Golgi 0.0022 0.0002 0.0011 0.0001 0.0005 0.0002 0.0039 0.0003 0.0022 0.003 0.003 0.0009 0.0012 0.0001 0.0009 0.0018 0.0034 0.0005
Lipid Particles 0.0023 0.0001 0.0015 0.0001 0.0003 0.0003 0.0082 0.0004 0.0052 0.0109 0.0074 0.0007 0.0029 0.0001 0.0012 0.0046 0.0184 0.0021
Mitochondria 0.0006 0.0001 0.0021 0.0002 0.0005 0.0001 0.0041 0.0001 0.0068 0.0011 0.0262 0.0048 0.0008 0 0.0003 0.0036 0.0031 0.0002
None 0.2886 0.1786 0.2012 0.2156 0.278 0.1833 0.1399 0.1524 0.1683 0.0451 0.0664 0.1166 0.1315 0.1081 0.1668 0.1703 0.1364 0.118
Nuclear Periphery 0.0028 0.0002 0.0011 0.0002 0.0012 0.0005 0.004 0.0004 0.0005 0.0071 0.0073 0.0026 0.002 0.0002 0.0014 0.0033 0.0155 0.0046
Nucleolus 0.0004 0.0001 0.0009 0 0.0005 0.0002 0.0025 0.0001 0.0001 0.003 0.0003 0.0002 0.0003 0 0.0002 0.0022 0.0037 0.0003
Nucleus 0.0013 0.0008 0.0023 0.0007 0.004 0.003 0.0033 0.001 0.0008 0.0181 0.0014 0.0008 0.0012 0.0005 0.0011 0.003 0.0231 0.0023
Peroxisomes 0.0069 0 0.0013 0.0001 0.0002 0.0001 0.0062 0.0001 0.0042 0.0019 0.0325 0.0011 0.0124 0 0.0008 0.0017 0.0244 0.0008
Punctate Nuclear 0.0027 0.0002 0.0019 0.0002 0.0028 0.0013 0.0149 0.0004 0.001 0.0173 0.0017 0.0012 0.0036 0.0002 0.0104 0.0029 0.0144 0.0133
Vacuole 0.0015 0.0009 0.0009 0.0005 0.0013 0.0006 0.0026 0.0011 0.0011 0.002 0.0024 0.0004 0.0012 0.0004 0.0006 0.002 0.0025 0.0003
Vacuole Periphery 0.0002 0 0.0002 0.0001 0.0002 0.0001 0.0005 0 0.0001 0.0006 0.0016 0.0002 0.0003 0 0.0001 0.0008 0.0013 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 12.5316 14.5632 9.9752 15.5086 14.2168 5.0099 12.6011 15.863 11.8718 13.8911
Translational Efficiency 0.435 0.369 0.8074 0.429 0.4255 0.8893 0.4961 0.455 0.6135 0.4594

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
2378 1609 1893 981 334 2160 1520 128 2712 3769 3413 1109

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 686.71 740.73 902.72 814.33 798.21 735.37 942.05 924.57 700.44 737.66 920.24 827.05
Standard Deviation 71.76 116.24 143.12 109.78 92.90 92.67 107.87 131.69 83.19 103.43 130.10 117.91
Intensity Change Log 2 — 0.109247 0.394578 0.245913 — -0.118298 0.239035 0.212014 — -0.008595 0.313056 0.227790

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000451 0.000457 0.001073 0.003760 0.000490 0.000998 0.001792 0.008468 0.000456 0.000767 0.001393 0.004304
Bud Neck 0.025816 0.025039 0.009241 0.015342 0.009255 0.035094 0.030049 0.023371 0.023777 0.030802 0.018508 0.016268
Bud Site 0.013110 0.020286 0.042637 0.043139 0.007879 0.022488 0.083096 0.030842 0.012466 0.021548 0.060655 0.041720
Cell Periphery 0.000651 0.000124 0.000256 0.001503 0.001261 0.000206 0.001197 0.000854 0.000726 0.000171 0.000675 0.001428
Cytoplasm 0.578992* 0.511235* 0.654957* 0.452701* 0.547060* 0.468109* 0.531508* 0.421048* 0.575059* 0.486520* 0.599979* 0.449048*
Cytoplasmic Foci 0.138256 0.208473* 0.023658 0.044573 0.236951* 0.215939* 0.037704 0.035830 0.150411 0.212752* 0.029914 0.043564
Eisosomes 0.001050 0.000129 0.000043 0.000550 0.001037 0.000244 0.000228 0.000282 0.001049 0.000195 0.000126 0.000519
Endoplasmic Reticulum 0.000736 0.001020 0.002447 0.003960 0.001634 0.000717 0.002655 0.004469 0.000847 0.000846 0.002540 0.004019
Endosome 0.005427 0.025454 0.001698 0.004426 0.010548 0.028248 0.002952 0.003263 0.006058 0.027055 0.002257 0.004292
Golgi 0.003438 0.004579 0.000346 0.001974 0.001918 0.006823 0.000156 0.000121 0.003250 0.005865 0.000261 0.001760
Lipid Particles 0.005044 0.004280 0.000788 0.002315 0.006991 0.005785 0.001615 0.002698 0.005284 0.005142 0.001156 0.002359
Mitochondria 0.001784 0.005278 0.000313 0.001479 0.002375 0.008443 0.000244 0.000165 0.001857 0.007092 0.000282 0.001327
Mitotic Spindle 0.001117 0.005070 0.001942 0.007511 0.000639 0.009683 0.002159 0.005323 0.001058 0.007714 0.002039 0.007258
None 0.008037 0.003832 0.003424 0.001254 0.000764 0.001924 0.001523 0.002927 0.007142 0.002738 0.002577 0.001447
Nuclear Periphery 0.000116 0.000211 0.000304 0.000476 0.000200 0.000271 0.000405 0.000624 0.000126 0.000245 0.000349 0.000493
Nuclear Periphery Foci 0.000663 0.000828 0.002252 0.002295 0.001791 0.000627 0.001960 0.002844 0.000802 0.000713 0.002122 0.002358
Nucleolus 0.000960 0.001521 0.000173 0.000135 0.000330 0.001376 0.000154 0.000095 0.000883 0.001438 0.000164 0.000130
Nucleus 0.142871* 0.062917 0.195710* 0.182066* 0.089691 0.068226 0.145904* 0.117890 0.136321* 0.065960 0.173529* 0.174659*
Peroxisomes 0.002921 0.007015 0.000802 0.002652 0.001738 0.011155 0.000731 0.001252 0.002775 0.009388 0.000770 0.002490
Vacuole 0.068165 0.110135 0.057762 0.227587* 0.077077 0.111563 0.153843 0.337546* 0.069263 0.110953 0.100553 0.240279*
Vacuole Periphery 0.000392 0.002116 0.000174 0.000302 0.000372 0.002081 0.000122 0.000087 0.000390 0.002096 0.000151 0.000277

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -0.03 -4.50 -6.18 -6.02 -4.29 -1.68 -6.00 -1.29 -1.12 -0.90 -1.87 -8.29 -5.37 -4.56 -3.50
Bud Neck 0.46 11.15 7.45 6.06 -2.11 -10.12 -5.87 -0.44 4.22 2.43 -4.11 4.93 6.69 10.45 2.51
Bud Site -3.95 -8.32 -5.98 -3.41 1.88 -6.11 -12.96 -3.38 -0.13 7.77 -6.51 -14.84 -6.53 -3.14 6.15
Cell Periphery 14.45 9.81 -3.91 -9.03 -7.68 5.86 1.13 2.94 -4.64 3.14 13.87 2.30 -3.08 -9.08 -4.30
Cytoplasm 8.48 -5.80 20.24 11.13 22.53 5.97 3.92 9.06 6.66 7.50 14.70 1.81 22.01 11.74 19.87
Cytoplasmic Foci -12.30 37.71 29.07 32.23 -5.85 2.16 22.27 21.57 32.37 1.72 -14.44 41.07 34.24 47.13 -3.30
Eisosomes 15.42 17.13 7.95 -5.66 -7.38 3.72 3.84 3.62 -0.59 -1.50 14.61 16.02 8.67 -4.58 -6.20
Endoplasmic Reticulum -1.24 -9.04 -14.61 -10.67 -4.85 3.12 -5.00 -5.30 -6.78 -3.50 0.04 -13.81 -15.47 -14.69 -5.93
Endosome -13.55 10.91 3.14 13.98 -1.99 -11.46 9.86 8.95 18.18 0.17 -20.56 12.60 4.53 20.84 -1.34
Golgi -1.99 12.16 3.38 4.22 -1.16 -7.63 5.51 5.57 12.05 1.13 -5.87 13.67 3.69 6.99 -1.26
Lipid Particles 1.60 11.36 6.79 5.09 -11.99 1.37 7.33 5.21 4.67 -3.93 0.31 12.00 7.96 7.55 -10.17
Mitochondria -3.76 10.90 2.35 4.46 -1.67 -2.79 1.09 1.09 8.25 0.01 -7.05 5.99 2.52 8.24 -1.76
Mitotic Spindle -2.94 -1.32 -2.77 -0.24 -2.28 -5.70 -1.71 -1.74 0.18 -1.38 -6.04 -1.70 -3.22 1.10 -2.53
None 4.03 6.82 9.34 3.04 8.71 -3.79 -2.65 -2.86 -1.16 -1.92 6.01 7.52 8.80 3.02 4.18
Nuclear Periphery -4.41 -18.09 -16.92 -12.27 -8.43 -1.62 -10.80 -7.27 -6.57 -4.41 -5.15 -24.35 -18.28 -12.52 -7.92
Nuclear Periphery Foci -1.52 -9.92 -6.25 -5.13 0.33 3.75 -2.11 -3.15 -5.19 -2.12 0.27 -12.47 -6.71 -5.97 -0.39
Nucleolus -2.20 9.86 9.91 5.14 0.85 -5.47 4.76 5.58 6.59 1.86 -3.51 10.32 10.22 8.15 0.76
Nucleus 22.62 -13.17 -7.63 -20.32 2.60 4.06 -11.02 -3.15 -4.82 1.65 24.33 -13.25 -8.24 -20.94 0.50
Peroxisomes -8.07 8.19 2.80 8.95 -2.37 -11.99 4.45 2.37 12.57 -0.19 -12.94 9.82 3.06 13.43 -2.59
Vacuole -11.42 -3.09 -27.14 -21.17 -24.99 -5.60 -16.09 -13.83 -12.77 -9.54 -14.63 -17.47 -29.92 -24.61 -21.75
Vacuole Periphery -5.38 7.91 3.25 5.94 -0.34 -6.80 4.47 4.89 8.18 1.95 -8.70 11.44 3.82 9.49 -0.61
Standard name
Human Ortholog
Description Protein proposed to regulate endocytosis of plasma membrane proteins; regulates by recruiting the ubiquitin ligase Rsp5p to its target in the plasma membrane; SWAT-GFP and mCherry fusion proteins localize to the cytosol
Localization
Cell Percentages cytoplasm (77%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-10

Art5

Art5


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Art5-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available