Standard name | |
---|---|
Human Ortholog | |
Description | Component of the nonsense-mediated mRNA decay (NMD) pathway; along with Nam7p and Nmd2p; involved in decay of mRNA containing nonsense codons; involved in telomere maintenance |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 1 | 1 | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 2 | 0 | 1 | 0 |
Bud | 1 | 1 | 0 | 0 | 3 | 4 | 14 | 5 | 8 | 24 | 24 | 21 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 3 | 10 | 4 | 7 | 2 | 12 | 6 | 6 | 13 | 13 | 30 | 1 | 14 | 4 | 14 | 25 | 15 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 262 | 156 | 294 | 214 | 266 | 262 | 297 | 226 | 192 | 346 | 281 | 309 | 279 | 398 | 371 | 216 | 278 | 230 | 253 | 152 | 276 | 111 | 190 | 257 |
Endoplasmic Reticulum | 1 | 4 | 8 | 2 | 3 | 1 | 4 | 3 | 6 | 7 | 2 | 2 | 4 | 2 | 0 | 8 | 10 | 12 | 4 | 1 | 18 | 3 | 2 | 6 |
Endosome | 0 | 0 | 1 | 0 | 2 | 1 | 1 | 8 | 0 | 1 | 3 | 1 | 0 | 3 | 1 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 1 | 1 |
Golgi | 0 | 1 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 2 | 0 | 0 | 0 | 2 | 0 | 1 | 0 |
Mitochondria | 5 | 3 | 15 | 72 | 86 | 183 | 263 | 246 | 260 | 414 | 351 | 412 | 1 | 0 | 0 | 5 | 5 | 3 | 0 | 1 | 11 | 1 | 6 | 1 |
Nucleus | 0 | 0 | 0 | 2 | 0 | 2 | 1 | 1 | 2 | 1 | 3 | 2 | 0 | 1 | 1 | 3 | 1 | 3 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 2 | 3 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vac/Vac Membrane | 1 | 1 | 4 | 1 | 2 | 2 | 13 | 9 | 19 | 21 | 18 | 23 | 1 | 1 | 1 | 9 | 7 | 10 | 1 | 7 | 3 | 6 | 3 | 2 |
Unique Cell Count | 263 | 164 | 310 | 238 | 311 | 340 | 452 | 397 | 383 | 603 | 514 | 598 | 284 | 403 | 374 | 234 | 293 | 244 | 267 | 168 | 321 | 126 | 215 | 274 |
Labelled Cell Count | 270 | 169 | 333 | 297 | 371 | 458 | 606 | 505 | 495 | 827 | 700 | 807 | 287 | 420 | 378 | 256 | 328 | 274 | 267 | 168 | 321 | 126 | 215 | 274 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 6.2 | 8.2 | 6.5 | 6.0 | 5.7 | 4.9 | 4.7 | 4.6 | 4.3 | 4.3 | 4.0 | 4.0 | 7.8 | 7.6 | 7.5 | 9.4 | 9.3 | 9.4 | 7.0 | 8.0 | 8.8 |
Std Deviation (1e-4) | 0.6 | 1.0 | 1.1 | 1.3 | 1.4 | 1.6 | 1.3 | 1.1 | 1.2 | 1.0 | 0.9 | 0.8 | 1.2 | 0.9 | 1.3 | 1.7 | 1.3 | 1.3 | 1.1 | 0.9 | 1.6 |
Intensity Change (Log2) | – | – | – | -0.11 | -0.18 | -0.39 | -0.47 | -0.5 | -0.58 | -0.6 | -0.69 | -0.7 | 0.27 | 0.23 | 0.21 | 0.54 | 0.53 | 0.54 | 0.12 | 0.31 | 0.44 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | -1.1 | -0.7 | -2.5 | 0 | 0 | 0 | 0 | 0 | 0 | -2.6 | 0.2 | -2.0 | 1.6 | 2.8 | 1.6 |
Cytoplasm | -2.2 | -3.9 | -6.4 | -9.5 | -11.3 | -12.8 | -11.7 | -12.2 | -13.1 | 2.2 | 3.1 | 3.5 | -1.2 | 0 | -0.3 |
Endoplasmic Reticulum | 0 | -1.5 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | -1.0 | 0 | 0 | 0.6 | 0.6 | 1.5 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 8.1 | 7.7 | 13.6 | 0 | 0 | 0 | 0 | 0 | 0 | -3.4 | -4.5 | -4.3 | -1.7 | -2.1 | -2.4 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 0 | 0 | 1.5 | 1.0 | 2.7 | 1.9 | 1.9 | 2.1 | 0 | 0 | 0 | 1.9 | 1.0 | 2.1 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 1.7699 | 2.5818 | 2.2789 | 2.1997 | 2.4704 | 2.4778 | 2.0073 | 2.7967 | 2.5593 | 2.2143 | 2.3919 | 2.4564 | 1.9005 | 2.5526 | 2.4008 | 2.3219 | 1.8981 | 2.0146 |
Actin | 0.0112 | 0.0034 | 0.0065 | 0.0006 | 0.0007 | 0.0075 | 0.0125 | 0.0011 | 0.009 | 0.0013 | 0.0273 | 0.0014 | 0.0293 | 0.0004 | 0.0105 | 0.0088 | 0.0423 | 0.0019 |
Bud | 0.0006 | 0.0098 | 0.0058 | 0.0007 | 0.0005 | 0.0016 | 0.002 | 0.0019 | 0.0031 | 0.0003 | 0.0027 | 0.0002 | 0.001 | 0.0044 | 0.0027 | 0.0029 | 0.0015 | 0.0007 |
Bud Neck | 0.0008 | 0.0007 | 0.0006 | 0.0006 | 0.0007 | 0.0013 | 0.0056 | 0.0005 | 0.0011 | 0.0019 | 0.0015 | 0.0007 | 0.0038 | 0.0002 | 0.0021 | 0.0016 | 0.003 | 0.0009 |
Bud Periphery | 0.0008 | 0.0029 | 0.0032 | 0.001 | 0.0005 | 0.0037 | 0.003 | 0.0012 | 0.0025 | 0.0001 | 0.0015 | 0.0002 | 0.0011 | 0.0017 | 0.0015 | 0.0042 | 0.002 | 0.0005 |
Bud Site | 0.0082 | 0.0364 | 0.0046 | 0.0002 | 0.0003 | 0.0007 | 0.0044 | 0.0174 | 0.0079 | 0.0058 | 0.0051 | 0.0002 | 0.0036 | 0.0032 | 0.0069 | 0.0181 | 0.015 | 0.0003 |
Cell Periphery | 0.0007 | 0.0006 | 0.0004 | 0.0004 | 0.0002 | 0.0002 | 0.0004 | 0.0003 | 0.0003 | 0.0001 | 0.0002 | 0 | 0.0005 | 0.0002 | 0.0001 | 0.0008 | 0.0006 | 0.0001 |
Cytoplasm | 0.6992 | 0.7654 | 0.7675 | 0.8149 | 0.702 | 0.7652 | 0.6527 | 0.8896 | 0.8053 | 0.873 | 0.7276 | 0.8876 | 0.6935 | 0.9259 | 0.8543 | 0.7655 | 0.7306 | 0.8922 |
Cytoplasmic Foci | 0.0446 | 0.0245 | 0.032 | 0.0294 | 0.0364 | 0.0352 | 0.0597 | 0.0184 | 0.0373 | 0.0374 | 0.036 | 0.0225 | 0.0593 | 0.0155 | 0.0267 | 0.0293 | 0.0487 | 0.0291 |
Eisosomes | 0.0002 | 0.0001 | 0.0001 | 0 | 0 | 0.0001 | 0.0001 | 0.0001 | 0 | 0 | 0.0001 | 0 | 0.0003 | 0 | 0.0001 | 0.0002 | 0.0006 | 0 |
Endoplasmic Reticulum | 0.0066 | 0.0039 | 0.0044 | 0.0026 | 0.0177 | 0.0031 | 0.0144 | 0.0043 | 0.0057 | 0.0026 | 0.0029 | 0.0039 | 0.0109 | 0.0026 | 0.0029 | 0.0049 | 0.0042 | 0.004 |
Endosome | 0.0437 | 0.0261 | 0.0374 | 0.028 | 0.0429 | 0.0456 | 0.1111 | 0.015 | 0.049 | 0.0507 | 0.0712 | 0.0321 | 0.0738 | 0.0155 | 0.0392 | 0.0542 | 0.0665 | 0.0388 |
Golgi | 0.006 | 0.0033 | 0.0036 | 0.0009 | 0.0017 | 0.0034 | 0.0221 | 0.0016 | 0.0098 | 0.003 | 0.0436 | 0.0068 | 0.0152 | 0.0016 | 0.0066 | 0.011 | 0.0094 | 0.0033 |
Lipid Particles | 0.0054 | 0.0005 | 0.0013 | 0.0001 | 0.0003 | 0.0008 | 0.0151 | 0.0004 | 0.0021 | 0.0008 | 0.0292 | 0.004 | 0.0137 | 0.0001 | 0.0037 | 0.0069 | 0.0141 | 0.0004 |
Mitochondria | 0.0027 | 0.0014 | 0.003 | 0.0007 | 0.0012 | 0.0026 | 0.0137 | 0.0006 | 0.0039 | 0.0007 | 0.0138 | 0.0081 | 0.0105 | 0.0011 | 0.0033 | 0.0381 | 0.0124 | 0.0083 |
None | 0.1471 | 0.109 | 0.1155 | 0.111 | 0.182 | 0.116 | 0.0362 | 0.0388 | 0.0413 | 0.0093 | 0.012 | 0.0229 | 0.0443 | 0.0221 | 0.0149 | 0.0118 | 0.0207 | 0.0128 |
Nuclear Periphery | 0.0017 | 0.0008 | 0.0009 | 0.0006 | 0.0012 | 0.001 | 0.0085 | 0.0006 | 0.0042 | 0.001 | 0.0023 | 0.0009 | 0.0053 | 0.0006 | 0.001 | 0.0015 | 0.0014 | 0.0005 |
Nucleolus | 0.0004 | 0.0003 | 0.0003 | 0.0001 | 0.0001 | 0.0001 | 0.0004 | 0.0001 | 0.0003 | 0.0001 | 0.0002 | 0.0001 | 0.001 | 0 | 0.0002 | 0.0099 | 0.0003 | 0 |
Nucleus | 0.0015 | 0.0015 | 0.0015 | 0.0012 | 0.0013 | 0.0011 | 0.0067 | 0.0012 | 0.0019 | 0.0016 | 0.0014 | 0.0011 | 0.0013 | 0.0009 | 0.0011 | 0.0035 | 0.0013 | 0.0007 |
Peroxisomes | 0.0087 | 0.0005 | 0.0009 | 0.0002 | 0.0004 | 0.004 | 0.0123 | 0.0005 | 0.0042 | 0.0013 | 0.0143 | 0.0033 | 0.0138 | 0.0001 | 0.0141 | 0.0129 | 0.0106 | 0.0018 |
Punctate Nuclear | 0.0019 | 0.001 | 0.0014 | 0.0003 | 0.0009 | 0.0008 | 0.0033 | 0.0005 | 0.002 | 0.0008 | 0.0028 | 0.0008 | 0.0102 | 0.0001 | 0.0014 | 0.0014 | 0.0033 | 0.0004 |
Vacuole | 0.0069 | 0.0069 | 0.0077 | 0.0052 | 0.0074 | 0.0048 | 0.0117 | 0.0055 | 0.0074 | 0.0066 | 0.0027 | 0.0024 | 0.0059 | 0.0033 | 0.0055 | 0.01 | 0.0092 | 0.0025 |
Vacuole Periphery | 0.001 | 0.0009 | 0.0014 | 0.0011 | 0.0015 | 0.0011 | 0.0043 | 0.0005 | 0.0017 | 0.0014 | 0.0012 | 0.0008 | 0.0019 | 0.0005 | 0.0012 | 0.0024 | 0.0023 | 0.0009 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 14.6931 | 14.4058 | 10.6898 | 12.2211 | 9.2179 | 14.2113 | 12.8521 | 13.5898 | 11.0179 | 13.2041 |
Translational Efficiency | 1.4159 | 1.2338 | 1.4361 | 1.1407 | 1.6285 | 1.2198 | 1.3198 | 1.0264 | 1.3422 | 1.1833 |
Dataset | Hit |
---|---|
Protein Concentration | ✔ |
Protein Localization | ✘ |
Gene Expression | ✔ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
1668 | 1489 | 191 | 1342 | 1304 | 940 | 1153 | 97 | 2972 | 2429 | 1344 | 1439 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 859.32 | 908.38 | 1102.71 | 931.10 | 851.05 | 905.26 | 1007.34 | 1153.00 | 855.69 | 907.17 | 1020.89 | 946.06 |
Standard Deviation | 106.62 | 229.97 | 119.99 | 134.73 | 105.50 | 118.25 | 124.65 | 151.16 | 106.21 | 194.51 | 128.39 | 146.85 |
Intensity Change Log 2 | — | 0.080100 | 0.359786 | 0.115741 | — | 0.089088 | 0.243235 | 0.438077 | — | 0.084580 | 0.302969 | 0.285116 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.000237 | 0.000186 | 0.000705 | 0.000381 | 0.000192 | 0.000240 | 0.000166 | 0.002615 | 0.000217 | 0.000207 | 0.000242 | 0.000532 |
Bud Neck | 0.004001 | 0.003308 | 0.002130 | 0.002095 | 0.003371 | 0.006224 | 0.000931 | 0.002675 | 0.003725 | 0.004437 | 0.001102 | 0.002134 |
Bud Site | 0.015136 | 0.024643 | 0.018374 | 0.027394 | 0.015605 | 0.022370 | 0.007354 | 0.027958 | 0.015342 | 0.023763 | 0.008920 | 0.027432 |
Cell Periphery | 0.000192 | 0.000186 | 0.000235 | 0.000111 | 0.000204 | 0.000160 | 0.000062 | 0.000108 | 0.000197 | 0.000176 | 0.000086 | 0.000111 |
Cytoplasm | 0.520446* | 0.649806* | 0.572803* | 0.704499* | 0.598250* | 0.554172* | 0.747874* | 0.444774* | 0.554583* | 0.612797* | 0.722994* | 0.686992* |
Cytoplasmic Foci | 0.307876* | 0.196920* | 0.106070 | 0.088253 | 0.267461* | 0.216988* | 0.070821 | 0.167514 | 0.290143* | 0.204686* | 0.075830 | 0.093595 |
Eisosomes | 0.000092 | 0.000022 | 0.000058 | 0.000016 | 0.000068 | 0.000059 | 0.000017 | 0.000045 | 0.000082 | 0.000036 | 0.000023 | 0.000018 |
Endoplasmic Reticulum | 0.005875 | 0.001316 | 0.002396 | 0.000479 | 0.007359 | 0.002784 | 0.000869 | 0.000336 | 0.006526 | 0.001884 | 0.001086 | 0.000469 |
Endosome | 0.026752 | 0.019507 | 0.017308 | 0.007795 | 0.016666 | 0.028016 | 0.006509 | 0.020675 | 0.022327 | 0.022800 | 0.008043 | 0.008663 |
Golgi | 0.022358 | 0.010229 | 0.000592 | 0.001508 | 0.019720 | 0.009323 | 0.000544 | 0.001247 | 0.021201 | 0.009878 | 0.000551 | 0.001491 |
Lipid Particles | 0.004103 | 0.001459 | 0.002935 | 0.000871 | 0.003338 | 0.002757 | 0.000983 | 0.001601 | 0.003768 | 0.001961 | 0.001260 | 0.000920 |
Mitochondria | 0.004249 | 0.002092 | 0.000055 | 0.000267 | 0.003671 | 0.001862 | 0.000398 | 0.008394 | 0.003996 | 0.002003 | 0.000349 | 0.000815 |
Mitotic Spindle | 0.000944 | 0.002777 | 0.003085 | 0.003184 | 0.000197 | 0.002937 | 0.001996 | 0.006482 | 0.000616 | 0.002839 | 0.002151 | 0.003406 |
None | 0.000532 | 0.002665 | 0.000394 | 0.001328 | 0.000779 | 0.001796 | 0.001281 | 0.000277 | 0.000640 | 0.002329 | 0.001155 | 0.001258 |
Nuclear Periphery | 0.000065 | 0.000077 | 0.000401 | 0.000067 | 0.000067 | 0.000137 | 0.000266 | 0.000122 | 0.000066 | 0.000100 | 0.000285 | 0.000070 |
Nuclear Periphery Foci | 0.000318 | 0.000440 | 0.012084 | 0.001153 | 0.000385 | 0.000362 | 0.003566 | 0.002658 | 0.000347 | 0.000410 | 0.004776 | 0.001254 |
Nucleolus | 0.000308 | 0.000366 | 0.000116 | 0.000142 | 0.000134 | 0.000268 | 0.000147 | 0.000059 | 0.000231 | 0.000328 | 0.000143 | 0.000136 |
Nucleus | 0.008109 | 0.019004 | 0.030744 | 0.010731 | 0.008092 | 0.011344 | 0.031209 | 0.006258 | 0.008101 | 0.016039 | 0.031143 | 0.010430 |
Peroxisomes | 0.001517 | 0.001451 | 0.000789 | 0.001406 | 0.001014 | 0.001372 | 0.000359 | 0.011027 | 0.001297 | 0.001420 | 0.000420 | 0.002054 |
Vacuole | 0.066891 | 0.057250 | 0.228186* | 0.147246 | 0.043050 | 0.130085 | 0.124318 | 0.294475* | 0.056430 | 0.085436 | 0.139079 | 0.157170 |
Vacuole Periphery | 0.010000 | 0.006296 | 0.000542 | 0.001076 | 0.010376 | 0.006746 | 0.000331 | 0.000699 | 0.010165 | 0.006470 | 0.000361 | 0.001050 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | 2.46 | -4.30 | -4.30 | -6.24 | 3.17 | -0.72 | 0.83 | -1.28 | -1.21 | -1.30 | 0.34 | -1.41 | -3.55 | -3.46 | -2.72 |
Bud Neck | 1.71 | 5.32 | 6.85 | 4.58 | 1.27 | -2.83 | 7.28 | 3.30 | 4.24 | -4.00 | -1.63 | 11.85 | 7.27 | 5.84 | -5.51 |
Bud Site | -6.56 | -1.53 | -4.33 | 0.35 | -1.79 | -2.98 | 7.41 | -1.89 | 0.04 | -4.35 | -6.74 | 7.02 | -4.52 | 0.02 | -7.72 |
Cell Periphery | -0.23 | -1.53 | 4.23 | 3.16 | 6.79 | 1.38 | 8.12 | 6.07 | 3.08 | -1.44 | 0.49 | 8.14 | 6.14 | 3.81 | -1.66 |
Cytoplasm | -13.75 | -0.82 | -17.25 | -3.26 | -7.00 | 3.94 | -11.83 | 6.26 | 4.67 | 10.15 | -7.96 | -16.09 | -12.95 | -5.66 | 2.62 |
Cytoplasmic Foci | 16.14 | 25.99 | 39.89 | 19.85 | 1.65 | 6.36 | 34.82 | 11.69 | 7.57 | -3.96 | 16.42 | 51.14 | 45.22 | 25.04 | -4.06 |
Eisosomes | 11.42 | 4.26 | 12.45 | 3.70 | 10.12 | 0.93 | 11.33 | 6.38 | 2.44 | -4.50 | 8.32 | 14.36 | 16.07 | 4.71 | 4.59 |
Endoplasmic Reticulum | 7.53 | 5.26 | 9.38 | 4.84 | 7.04 | 4.43 | 7.09 | 7.72 | 5.45 | 4.78 | 8.28 | 10.42 | 11.72 | 6.79 | 5.38 |
Endosome | 4.42 | 6.12 | 16.36 | 9.55 | 5.69 | -6.87 | 11.94 | 1.95 | 5.21 | -1.62 | -0.35 | 18.19 | 17.18 | 14.73 | 0.04 |
Golgi | 11.39 | 25.88 | 22.96 | 11.76 | -1.76 | 8.98 | 23.41 | 22.38 | 9.82 | -0.41 | 14.47 | 34.06 | 29.20 | 13.81 | -1.85 |
Lipid Particles | 11.36 | 4.02 | 16.60 | 3.72 | 7.04 | 1.59 | 10.38 | 4.61 | 3.00 | -0.88 | 8.92 | 15.74 | 18.60 | 6.64 | 2.50 |
Mitochondria | 2.48 | 5.75 | 5.47 | 3.78 | -1.37 | 2.73 | 5.68 | 0.17 | -0.41 | -0.91 | 3.46 | 7.24 | 6.78 | 3.92 | -0.48 |
Mitotic Spindle | -1.80 | -1.58 | -2.52 | -0.23 | -0.23 | -2.81 | -2.08 | -1.19 | 0.01 | -0.30 | -3.05 | -1.95 | -3.16 | -0.23 | -0.73 |
None | -4.22 | 1.19 | -1.24 | 2.47 | -1.52 | -1.26 | -1.55 | 3.45 | 1.85 | 3.45 | -3.88 | -2.01 | -0.94 | 2.25 | 0.09 |
Nuclear Periphery | -1.13 | -10.87 | -2.25 | -0.66 | 10.44 | -1.79 | -6.19 | -2.84 | -0.59 | 4.12 | -2.02 | -9.76 | -1.95 | 0.72 | 9.60 |
Nuclear Periphery Foci | -1.91 | -10.67 | -9.63 | -7.11 | 9.81 | 0.10 | -9.91 | -5.04 | -5.59 | 2.08 | -0.65 | -15.17 | -7.69 | -8.84 | 11.87 |
Nucleolus | -0.43 | 1.43 | 1.24 | 3.11 | -1.24 | -2.45 | -0.61 | 6.58 | 3.85 | 6.19 | -1.09 | 1.23 | 1.26 | 3.78 | 0.21 |
Nucleus | -5.01 | -9.40 | -6.08 | 3.09 | 7.85 | -3.49 | -16.65 | 1.56 | 4.02 | 16.22 | -5.72 | -18.69 | -6.61 | 2.87 | 15.25 |
Peroxisomes | 0.44 | 3.80 | 1.84 | 1.45 | -2.32 | -3.18 | 9.78 | -1.27 | -1.15 | -1.50 | -1.05 | 10.31 | -0.68 | -0.19 | -4.61 |
Vacuole | 2.82 | -12.33 | -18.23 | -19.52 | 5.33 | -14.38 | -18.81 | -12.65 | -9.49 | -8.52 | -9.19 | -20.23 | -22.76 | -16.73 | -2.40 |
Vacuole Periphery | 4.50 | 14.50 | 13.66 | 10.16 | -2.49 | 3.18 | 14.55 | 14.06 | 6.85 | -0.25 | 5.54 | 20.65 | 18.87 | 11.40 | -3.85 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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