Standard name | |
---|---|
Human Ortholog | |
Description | Mitochondrial ribosomal protein of the large subunit; mutation confers increased replicative lifespan |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 1 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 3 | 5 | 0 | 6 | 13 | 11 |
Bud | 4 | 0 | 5 | 1 | 3 | 3 | – | 4 | 9 | 8 | 6 | 8 | 4 | 4 | 2 | 2 | 1 | 0 | 0 | 0 | 0 | 0 | 2 | 3 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3 | 0 |
Bud Site | 0 | 0 | 0 | 1 | 0 | 2 | – | 2 | 1 | 2 | 4 | 4 | 1 | 1 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 2 | 2 | 0 | 2 | 1 | – | 1 | 1 | 5 | 2 | 2 | 9 | 7 | 16 | 3 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 0 |
Cytoplasm | 4 | 3 | 3 | 1 | 8 | 3 | – | 2 | 5 | 6 | 1 | 2 | 5 | 9 | 17 | 5 | 3 | 2 | 3 | 0 | 1 | 0 | 1 | 1 |
Endoplasmic Reticulum | 3 | 2 | 3 | 2 | 2 | 1 | – | 0 | 0 | 0 | 3 | 1 | 5 | 1 | 1 | 1 | 0 | 3 | 0 | 0 | 0 | 0 | 1 | 3 |
Endosome | 1 | 6 | 0 | 2 | 5 | 0 | – | 3 | 0 | 1 | 2 | 0 | 16 | 23 | 27 | 3 | 1 | 5 | 9 | 6 | 4 | 17 | 11 | 24 |
Golgi | 6 | 4 | 2 | 0 | 1 | 0 | – | 0 | 0 | 0 | 0 | 0 | 37 | 25 | 45 | 14 | 1 | 9 | 17 | 14 | 6 | 19 | 37 | 34 |
Mitochondria | 198 | 127 | 106 | 78 | 134 | 160 | – | 173 | 207 | 257 | 207 | 269 | 345 | 309 | 373 | 1 | 1 | 5 | 154 | 99 | 89 | 157 | 252 | 229 |
Nucleus | 1 | 0 | 1 | 0 | 1 | 2 | – | 4 | 3 | 4 | 1 | 2 | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 1 |
Nuclear Periphery | 6 | 4 | 6 | 2 | 9 | 16 | – | 17 | 21 | 20 | 20 | 24 | 22 | 10 | 7 | 4 | 1 | 1 | 1 | 2 | 0 | 2 | 2 | 7 |
Nucleolus | 8 | 2 | 3 | 5 | 11 | 18 | – | 26 | 19 | 20 | 21 | 28 | 8 | 8 | 6 | 4 | 2 | 4 | 0 | 0 | 0 | 2 | 3 | 3 |
Peroxisomes | 2 | 3 | 0 | 0 | 6 | 3 | – | 3 | 3 | 5 | 3 | 2 | 14 | 24 | 41 | 3 | 7 | 6 | 4 | 4 | 8 | 17 | 39 | 28 |
SpindlePole | 1 | 1 | 1 | 0 | 3 | 0 | – | 5 | 5 | 10 | 2 | 4 | 8 | 1 | 5 | 0 | 1 | 3 | 0 | 1 | 0 | 1 | 3 | 4 |
Vac/Vac Membrane | 30 | 32 | 9 | 0 | 12 | 12 | – | 6 | 7 | 14 | 6 | 9 | 33 | 32 | 60 | 8 | 1 | 7 | 12 | 6 | 1 | 5 | 8 | 14 |
Unique Cell Count | 210 | 146 | 109 | 81 | 158 | 175 | 200 | 225 | 287 | 223 | 283 | 384 | 356 | 454 | 42 | 19 | 36 | 214 | 144 | 113 | 236 | 390 | 372 | |
Labelled Cell Count | 265 | 186 | 141 | 92 | 197 | 221 | 246 | 281 | 352 | 278 | 355 | 508 | 455 | 600 | 48 | 20 | 46 | 214 | 144 | 113 | 236 | 390 | 372 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 7.5 | 8.6 | 6.1 | 7.0 | 7.6 | 6.3 | – | 6.0 | 5.6 | 5.7 | 5.9 | 5.4 | 8.0 | 8.1 | 8.2 | 9.7 | 9.7 | 9.1 | 8.0 | 8.4 | 8.3 |
Std Deviation (1e-4) | 1.4 | 1.6 | 1.5 | 3.3 | 1.8 | 1.9 | – | 1.6 | 2.0 | 1.6 | 2.2 | 1.5 | 1.6 | 1.7 | 1.8 | 2.2 | 2.0 | 2.3 | 2.0 | 2.0 | 1.9 |
Intensity Change (Log2) | – | – | – | 0.18 | 0.3 | 0.03 | – | -0.04 | -0.13 | -0.11 | -0.06 | -0.17 | 0.39 | 0.41 | 0.42 | 0.66 | 0.66 | 0.56 | 0.38 | 0.45 | 0.44 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 2.7 | 2.6 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 2.7 | 2.0 | 2.7 | 0 | 0 | 0 |
Mitochondria | -0.4 | -3.3 | -2.0 | – | -3.0 | -1.9 | -2.5 | -1.6 | -1.0 | -2.4 | -3.1 | -4.0 | -11.5 | -10.0 | -10.2 |
Nucleus | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0.1 | 1.1 | – | 1.0 | 1.2 | 0.5 | 1.1 | 1.0 | 0.1 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 1.5 | 2.4 | – | 3.0 | 2.0 | 1.6 | 2.2 | 2.3 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 2.8 | 3.3 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | -0.2 | -0.4 | – | -2.1 | -2.1 | -1.3 | 0 | -2.2 | 0.1 | 0.2 | 1.4 | 0 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 3.7144 | 3.9227 | 3.5777 | 3.0508 | 3.1432 | 3.5206 | 3.5289 | 3.6978 | 3.16 | 3.5372 | 2.9257 | 3.3437 | 4.4193 | 4.2878 | 4.2169 | 4.3901 | 3.3769 | 4.4154 |
Actin | 0.0464 | 0.0245 | 0.0163 | 0.0677 | 0.0266 | 0.0051 | 0.0131 | 0.0088 | 0.0138 | 0.0021 | 0.01 | 0.0047 | 0.0744 | 0.0214 | 0.0266 | 0.0039 | 0.0545 | 0.0014 |
Bud | 0.0004 | 0.0009 | 0.001 | 0.0006 | 0.0001 | 0.0002 | 0.0006 | 0.0009 | 0.0005 | 0.0005 | 0.0005 | 0.0003 | 0.0009 | 0.0011 | 0.0006 | 0.0094 | 0.0004 | 0 |
Bud Neck | 0.0007 | 0.0003 | 0.0006 | 0.0003 | 0.0002 | 0.0001 | 0.0004 | 0.0002 | 0.0005 | 0.0001 | 0.0002 | 0.0003 | 0.0016 | 0.0007 | 0.0005 | 0.0002 | 0.0001 | 0.0002 |
Bud Periphery | 0.0007 | 0.0016 | 0.0022 | 0.0011 | 0.0003 | 0.0003 | 0.0018 | 0.0017 | 0.0008 | 0.001 | 0.0009 | 0.0004 | 0.0013 | 0.0018 | 0.0016 | 0.0407 | 0.0008 | 0.0001 |
Bud Site | 0.0015 | 0.0132 | 0.0126 | 0.0016 | 0.0003 | 0.0004 | 0.001 | 0.0064 | 0.0019 | 0.0006 | 0.0012 | 0.0007 | 0.0064 | 0.012 | 0.0022 | 0.0014 | 0.0004 | 0.0001 |
Cell Periphery | 0.0003 | 0.0002 | 0.0009 | 0.0003 | 0.0001 | 0.0001 | 0.0003 | 0.0003 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0003 | 0.0009 | 0.0008 | 0.0002 | 0.0002 | 0.0001 |
Cytoplasm | 0.0022 | 0.0012 | 0.0044 | 0.0006 | 0.0001 | 0.0002 | 0.0028 | 0.0008 | 0.0005 | 0.0001 | 0.0007 | 0.0002 | 0.0012 | 0.0026 | 0.0009 | 0.0002 | 0.0001 | 0.0199 |
Cytoplasmic Foci | 0.013 | 0.015 | 0.0166 | 0.0157 | 0.0096 | 0.003 | 0.02 | 0.0108 | 0.015 | 0.0038 | 0.0117 | 0.0059 | 0.0259 | 0.0124 | 0.012 | 0.0262 | 0.0026 | 0.0033 |
Eisosomes | 0.001 | 0.0002 | 0.0007 | 0.0007 | 0.0001 | 0.0001 | 0.001 | 0.0003 | 0.0006 | 0.0001 | 0.0002 | 0.0003 | 0.0008 | 0.0005 | 0.0006 | 0.0002 | 0.0003 | 0.0001 |
Endoplasmic Reticulum | 0.0021 | 0.0001 | 0.0008 | 0.0021 | 0 | 0.0001 | 0.003 | 0.0003 | 0.0002 | 0.0001 | 0.0008 | 0.0005 | 0.0015 | 0.0009 | 0.0021 | 0.0001 | 0 | 0.0003 |
Endosome | 0.0332 | 0.0147 | 0.0277 | 0.0365 | 0.0091 | 0.006 | 0.0547 | 0.0277 | 0.0164 | 0.011 | 0.0472 | 0.0148 | 0.0317 | 0.0273 | 0.0154 | 0.0108 | 0.0412 | 0.0075 |
Golgi | 0.0291 | 0.0295 | 0.0362 | 0.0309 | 0.0293 | 0.018 | 0.0499 | 0.0452 | 0.0328 | 0.0085 | 0.0283 | 0.0343 | 0.0368 | 0.0449 | 0.023 | 0.0189 | 0.0117 | 0.0244 |
Lipid Particles | 0.0489 | 0.0272 | 0.0107 | 0.0151 | 0.0052 | 0.0032 | 0.0272 | 0.0084 | 0.0077 | 0.0076 | 0.0193 | 0.0091 | 0.0441 | 0.0199 | 0.0082 | 0.0485 | 0.0197 | 0.0187 |
Mitochondria | 0.7688 | 0.8157 | 0.8307 | 0.7971 | 0.8806 | 0.9548 | 0.7439 | 0.8592 | 0.8811 | 0.9382 | 0.836 | 0.897 | 0.6874 | 0.7969 | 0.8405 | 0.7497 | 0.8223 | 0.9006 |
None | 0.0109 | 0.0003 | 0.0045 | 0.0007 | 0.0001 | 0.0002 | 0.0057 | 0.0002 | 0.0004 | 0.0001 | 0.0009 | 0.0003 | 0.0032 | 0.0017 | 0.0006 | 0.0005 | 0.0001 | 0.0001 |
Nuclear Periphery | 0.0044 | 0.0001 | 0.0005 | 0.0017 | 0 | 0.0001 | 0.0158 | 0.0001 | 0.0002 | 0 | 0.0067 | 0.0033 | 0.0018 | 0.0006 | 0.0002 | 0.0001 | 0 | 0.0015 |
Nucleolus | 0.0013 | 0.0008 | 0.0003 | 0.0001 | 0 | 0.0001 | 0.0019 | 0.0006 | 0.0001 | 0.0001 | 0.0005 | 0.0003 | 0.0007 | 0.0004 | 0.0001 | 0.0003 | 0.0001 | 0.0001 |
Nucleus | 0.0026 | 0.0003 | 0.0006 | 0.0002 | 0 | 0.0002 | 0.0075 | 0.0002 | 0.0001 | 0.0001 | 0.0012 | 0.001 | 0.0004 | 0.0002 | 0.0001 | 0.0002 | 0 | 0.0101 |
Peroxisomes | 0.0242 | 0.0507 | 0.0281 | 0.0191 | 0.0369 | 0.0058 | 0.0331 | 0.0223 | 0.0251 | 0.0232 | 0.0239 | 0.0234 | 0.0748 | 0.05 | 0.061 | 0.0869 | 0.0392 | 0.0097 |
Punctate Nuclear | 0.0023 | 0.0001 | 0.0008 | 0.0008 | 0.0002 | 0.0002 | 0.0089 | 0.0001 | 0.0005 | 0.0001 | 0.0026 | 0.0007 | 0.0015 | 0.0004 | 0.0002 | 0.0006 | 0 | 0.0003 |
Vacuole | 0.0024 | 0.0022 | 0.0018 | 0.0037 | 0.0003 | 0.0005 | 0.0033 | 0.0031 | 0.0007 | 0.0013 | 0.0043 | 0.0007 | 0.0015 | 0.0017 | 0.0014 | 0.0008 | 0.0032 | 0.0004 |
Vacuole Periphery | 0.0037 | 0.0012 | 0.0019 | 0.0034 | 0.0008 | 0.0012 | 0.0039 | 0.0025 | 0.0008 | 0.0013 | 0.0027 | 0.0017 | 0.0017 | 0.0018 | 0.0014 | 0.0006 | 0.003 | 0.0011 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 16.295 | 20.8096 | 18.53 | 18.2995 | 9.0545 | 18.6126 | 19.9886 | 25.5618 | 24.4622 | 25.2471 |
Translational Efficiency | 2.3757 | 1.5476 | 1.9842 | 2.0398 | 3.8992 | 3.2664 | 2.5738 | 1.9792 | 2.3667 | 2.4531 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
1426 | 1239 | 219 | 1859 | 1699 | 1889 | 1925 | 1804 | 3125 | 3128 | 2144 | 3663 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 681.08 | 870.58 | 1269.53 | 1069.10 | 807.39 | 897.76 | 1064.95 | 1051.17 | 749.75 | 886.99 | 1085.85 | 1060.27 |
Standard Deviation | 109.44 | 152.58 | 191.36 | 183.74 | 121.92 | 140.68 | 150.99 | 167.35 | 132.31 | 146.12 | 167.47 | 176.09 |
Intensity Change Log 2 | — | 0.354153 | 0.898398 | 0.650501 | — | 0.153064 | 0.399448 | 0.380658 | — | 0.248566 | 0.649271 | 0.510418 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.001130 | 0.003687 | 0.002497 | 0.005622 | 0.001723 | 0.001452 | 0.004255 | 0.007653 | 0.001452 | 0.002337 | 0.004076 | 0.006623 |
Bud Neck | 0.005683 | 0.002181 | 0.006555 | 0.003550 | 0.001138 | 0.001585 | 0.002090 | 0.001961 | 0.003212 | 0.001821 | 0.002546 | 0.002768 |
Bud Site | 0.010618 | 0.008185 | 0.005242 | 0.017522 | 0.002850 | 0.003758 | 0.009177 | 0.015002 | 0.006395 | 0.005512 | 0.008775 | 0.016281 |
Cell Periphery | 0.001046 | 0.000376 | 0.000170 | 0.000528 | 0.000568 | 0.000242 | 0.000167 | 0.000824 | 0.000786 | 0.000295 | 0.000168 | 0.000674 |
Cytoplasm | 0.006063 | 0.001709 | 0.000802 | 0.003401 | 0.000637 | 0.001005 | 0.001561 | 0.002861 | 0.003113 | 0.001284 | 0.001484 | 0.003135 |
Cytoplasmic Foci | 0.021646 | 0.026975 | 0.066464 | 0.019886 | 0.011320 | 0.017212 | 0.031060 | 0.017507 | 0.016032 | 0.021079 | 0.034677 | 0.018714 |
Eisosomes | 0.000073 | 0.000093 | 0.000068 | 0.000071 | 0.000053 | 0.000060 | 0.000059 | 0.000089 | 0.000062 | 0.000073 | 0.000060 | 0.000080 |
Endoplasmic Reticulum | 0.000418 | 0.000293 | 0.000935 | 0.000622 | 0.000155 | 0.000187 | 0.000375 | 0.000472 | 0.000275 | 0.000229 | 0.000432 | 0.000548 |
Endosome | 0.064295 | 0.041157 | 0.047994 | 0.035436 | 0.025891 | 0.030283 | 0.038010 | 0.029827 | 0.043415 | 0.034590 | 0.039029 | 0.032673 |
Golgi | 0.042578 | 0.038215 | 0.062878 | 0.048718 | 0.020663 | 0.028292 | 0.046493 | 0.042445 | 0.030663 | 0.032222 | 0.048167 | 0.045629 |
Lipid Particles | 0.006995 | 0.008059 | 0.010941 | 0.001968 | 0.004776 | 0.004939 | 0.001330 | 0.002202 | 0.005788 | 0.006175 | 0.002312 | 0.002083 |
Mitochondria | 0.779986* | 0.804392* | 0.649436* | 0.781841* | 0.901069* | 0.873221* | 0.779961* | 0.819246* | 0.845816* | 0.845958* | 0.766628* | 0.800262* |
Mitotic Spindle | 0.002402 | 0.006515 | 0.055448 | 0.022801 | 0.001614 | 0.002950 | 0.032667 | 0.011540 | 0.001974 | 0.004362 | 0.034994 | 0.017255 |
None | 0.001900 | 0.000336 | 0.000663 | 0.000605 | 0.000177 | 0.000331 | 0.000241 | 0.000289 | 0.000963 | 0.000333 | 0.000284 | 0.000449 |
Nuclear Periphery | 0.000062 | 0.000082 | 0.005282 | 0.000746 | 0.000051 | 0.000050 | 0.000461 | 0.000376 | 0.000056 | 0.000063 | 0.000953 | 0.000564 |
Nuclear Periphery Foci | 0.000652 | 0.000158 | 0.004229 | 0.000570 | 0.000126 | 0.000211 | 0.000384 | 0.000437 | 0.000366 | 0.000190 | 0.000777 | 0.000504 |
Nucleolus | 0.001281 | 0.000199 | 0.001655 | 0.000476 | 0.000069 | 0.000402 | 0.000142 | 0.000158 | 0.000622 | 0.000322 | 0.000297 | 0.000320 |
Nucleus | 0.001902 | 0.000211 | 0.001218 | 0.000450 | 0.000075 | 0.000253 | 0.000179 | 0.000192 | 0.000909 | 0.000236 | 0.000285 | 0.000323 |
Peroxisomes | 0.037136 | 0.047924 | 0.017259 | 0.015419 | 0.022497 | 0.027532 | 0.026051 | 0.022696 | 0.029177 | 0.035610 | 0.025153 | 0.019003 |
Vacuole | 0.006757 | 0.005073 | 0.026071 | 0.019490 | 0.001796 | 0.003120 | 0.012205 | 0.014232 | 0.004060 | 0.003893 | 0.013621 | 0.016901 |
Vacuole Periphery | 0.007375 | 0.004182 | 0.034192 | 0.020278 | 0.002755 | 0.002917 | 0.013130 | 0.009992 | 0.004863 | 0.003418 | 0.015282 | 0.015212 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -4.65 | -2.18 | -4.41 | -1.68 | -2.63 | 0.85 | -3.84 | -5.46 | -5.81 | -2.78 | -3.14 | -4.61 | -6.93 | -5.58 | -2.76 |
Bud Neck | 3.33 | -0.65 | 1.77 | -1.85 | 1.92 | -0.98 | -2.72 | -2.05 | -0.87 | 0.49 | 2.57 | 1.08 | 0.71 | -2.18 | -0.36 |
Bud Site | 1.13 | 2.56 | -3.13 | -4.10 | -5.62 | -0.86 | -5.09 | -7.47 | -6.84 | -3.16 | 0.81 | -2.07 | -7.28 | -7.96 | -5.03 |
Cell Periphery | 3.36 | 4.56 | 2.37 | -1.27 | -3.35 | 1.58 | 1.97 | -0.75 | -2.08 | -2.37 | 3.40 | 4.38 | 0.55 | -2.53 | -3.44 |
Cytoplasm | 3.33 | 4.13 | 1.81 | -2.79 | -4.79 | -1.70 | -4.37 | -4.98 | -3.80 | -2.79 | 2.95 | 2.70 | -0.12 | -4.73 | -4.52 |
Cytoplasmic Foci | -1.92 | -5.41 | 0.95 | 2.88 | 5.69 | -3.93 | -10.00 | -3.95 | -0.20 | 6.21 | -3.50 | -9.26 | -2.00 | 1.60 | 7.88 |
Eisosomes | -2.85 | 0.72 | 0.57 | 3.24 | -0.34 | -1.52 | -1.65 | -4.91 | -3.93 | -4.11 | -2.76 | 0.62 | -3.91 | -1.42 | -4.30 |
Endoplasmic Reticulum | 1.87 | -1.73 | -1.70 | -2.82 | 1.01 | -1.02 | -5.12 | -3.08 | -2.69 | -0.88 | 1.30 | -3.00 | -3.49 | -4.07 | -1.29 |
Endosome | 5.46 | 2.59 | 7.43 | 1.74 | 2.33 | -1.73 | -4.67 | -1.55 | 0.18 | 3.20 | 3.69 | 1.70 | 4.68 | 0.94 | 2.92 |
Golgi | 1.21 | -2.32 | -1.62 | -2.77 | 1.61 | -3.28 | -9.02 | -7.38 | -4.63 | 1.16 | -0.75 | -6.38 | -6.33 | -5.63 | 0.85 |
Lipid Particles | -0.59 | -1.47 | 4.12 | 4.53 | 3.72 | -0.17 | 3.61 | 2.42 | 2.74 | -1.61 | -0.39 | 4.35 | 4.65 | 5.15 | 0.49 |
Mitochondria | -2.11 | 5.10 | -0.14 | 2.12 | -5.23 | 3.70 | 13.81 | 9.55 | 6.11 | -3.99 | -0.04 | 9.42 | 6.60 | 6.68 | -3.96 |
Mitotic Spindle | -3.07 | -5.51 | -9.87 | -6.73 | 3.33 | -2.60 | -13.39 | -8.28 | -6.99 | 8.24 | -3.93 | -14.38 | -12.64 | -9.76 | 6.86 |
None | 3.20 | 2.55 | 2.48 | -1.34 | 0.23 | -1.50 | -2.26 | -3.26 | 0.44 | -2.08 | 2.73 | 3.06 | 2.12 | -0.98 | -1.66 |
Nuclear Periphery | -0.94 | -3.04 | -6.02 | -5.81 | 2.63 | 0.01 | -3.57 | -2.03 | -2.04 | 0.42 | -0.61 | -4.37 | -5.19 | -5.12 | 1.70 |
Nuclear Periphery Foci | 2.72 | -1.97 | 0.51 | -4.74 | 2.03 | -1.62 | -3.63 | -1.71 | -1.16 | -0.27 | 1.94 | -1.86 | -0.96 | -3.02 | 1.24 |
Nucleolus | 2.87 | -0.39 | 2.00 | -1.80 | 1.18 | -2.65 | -2.03 | -1.21 | 1.71 | -0.21 | 1.62 | 1.58 | 1.59 | -0.03 | -0.14 |
Nucleus | 2.43 | 0.63 | 2.00 | -1.99 | 1.14 | -2.16 | -2.26 | -1.70 | 0.52 | 0.06 | 2.11 | 1.82 | 1.73 | -1.27 | -0.29 |
Peroxisomes | -2.25 | 3.66 | 6.38 | 7.93 | 0.39 | -1.68 | -1.25 | -0.09 | 1.61 | 1.18 | -2.42 | 1.48 | 4.52 | 6.94 | 2.69 |
Vacuole | 1.74 | -5.52 | -8.20 | -9.31 | 1.88 | -2.92 | -11.35 | -8.49 | -7.43 | -1.17 | 0.34 | -10.23 | -12.10 | -12.20 | -2.35 |
Vacuole Periphery | 3.41 | -5.94 | -8.25 | -11.35 | 2.99 | -0.39 | -9.90 | -8.10 | -7.85 | 2.38 | 3.01 | -9.49 | -11.54 | -13.98 | 0.04 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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