Standard name
Human Ortholog
Description Minor isoform of pyruvate decarboxylase; decarboxylates pyruvate to acetaldehyde, involved in amino acid catabolism; transcription is glucose- and ethanol-dependent, and is strongly induced during sulfur limitation
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.77 0.9 0.96 0.99 0.98 0.93 0.9 – 0.78 0.78 0.68 0.76 0.95 0.98 0.97 0.9 0.89 0.93 0.58 0.74 0.75 0.79 0.82 0.89
Endoplasmic Reticulum 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0.08 0.09 0.07 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.18 0 0 0 0 0.05 0.08 – 0.19 0.19 0.42 0.24 0 0 0 0 0 0 0.13 0 0.1 0 0 0
Nucleus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0.08 0.06 0 0.06 0.05 0
Nuclear Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.12 0.12 0 0 0 0.05 0.06 – 0.06 0.06 0 0 0 0 0 0 0.07 0 0.12 0.11 0.05 0.09 0.05 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 1 1 – 1 0 0 0 0 0 0 0 0 0 0 0 1 0 0 1
Bud 1 0 0 1 0 2 2 – 2 8 7 8 0 0 0 0 0 0 0 0 0 0 1 0
Bud Neck 0 0 1 6 1 1 12 – 4 4 3 5 0 1 0 0 1 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 – 2 1 1 2 0 0 0 0 0 0 – – – – – –
Cell Periphery 1 0 7 0 1 4 0 – 0 0 2 5 0 0 0 2 1 1 0 0 2 0 0 0
Cytoplasm 80 107 192 257 379 369 468 – 233 322 253 354 42 155 184 82 90 100 65 91 153 21 72 102
Endoplasmic Reticulum 1 0 2 0 0 0 3 – 1 0 4 3 0 0 1 7 9 8 3 0 1 0 0 0
Endosome 0 1 1 0 0 1 0 – 2 6 1 4 1 1 0 3 2 2 1 1 1 0 1 0
Golgi 0 0 0 0 0 0 1 – 0 0 0 0 0 0 0 0 0 1 1 1 0 0 0 0
Mitochondria 19 0 0 1 0 20 41 – 57 78 156 110 1 0 0 3 4 4 14 3 19 0 0 1
Nucleus 3 1 6 1 1 1 6 – 6 7 9 7 0 1 2 2 0 1 9 7 2 1 4 1
Nuclear Periphery 3 0 0 0 0 2 0 – 0 2 1 1 0 0 0 1 0 0 0 0 0 0 0 1
Nucleolus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 1 0 – 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 1 0 1 8 – 3 5 3 6 0 0 0 0 1 1 0 1 2 0 0 0
Vac/Vac Membrane 13 14 6 1 12 18 29 – 19 26 13 19 1 7 4 4 7 3 13 13 10 2 4 4
Unique Cell Count 104 119 200 260 388 396 522 299 411 372 464 44 158 189 91 101 107 113 123 205 27 88 115
Labelled Cell Count 121 123 215 268 394 421 571 331 459 453 524 45 165 191 104 115 121 113 123 205 27 88 115


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.7 4.9 5.1 4.9 6.6 5.6 5.3 – 4.5 4.7 4.1 4.4 5.4 5.5 5.3 6.2 5.6 5.6 – 4.6 5.3
Std Deviation (1e-4) 0.7 1.4 0.8 1.2 2.9 2.9 2.0 – 1.6 1.6 1.7 1.4 1.6 2.0 1.5 1.9 1.5 1.9 – 1.1 2.3
Intensity Change (Log2) – – – -0.04 0.36 0.13 0.06 – -0.16 -0.12 -0.3 -0.22 0.08 0.11 0.07 0.29 0.13 0.13 – -0.15 0.06


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration -0.1517 -0.3554 -0.3087 -0.3847 -0.6549 -0.0935 -1.4355 -0.79 -0.9408 -1.016 -0.4349 -0.8516 -1.0657 -0.5173 -1.0172 -0.8751 -0.7342 -0.9402
Actin 0.0248 0.0017 0.0093 0.0078 0.0344 0.007 0.0148 0.0019 0.0096 0.0004 0.0017 0.0051 0.02 0.0035 0.009 0.0004 0.0015 0.0055
Bud 0.0003 0.0011 0.0007 0.0001 0.008 0.001 0.0004 0.0014 0.0006 0.0001 0.0022 0.0002 0.0036 0.002 0.0008 0.0001 0.0002 0.0027
Bud Neck 0.0008 0.0044 0.0021 0.0002 0.0011 0.0009 0.0034 0.0002 0.0002 0.0003 0.0017 0.0009 0.0016 0.0005 0.0044 0.0002 0.0008 0.0014
Bud Periphery 0.0006 0.0013 0.0009 0.0001 0.0049 0.0011 0.0005 0.0033 0.0007 0.0001 0.0018 0.0006 0.0035 0.0029 0.0011 0.0001 0.0002 0.0025
Bud Site 0.0007 0.0116 0.0018 0.0001 0.0013 0.0002 0.0077 0.0139 0.0017 0.0002 0.0062 0.0017 0.0055 0.0076 0.0046 0.0001 0.0002 0.0003
Cell Periphery 0.0001 0.0002 0.0001 0 0.0003 0 0.0002 0.0003 0.0001 0 0.0002 0 0.0004 0.0001 0.0002 0 0 0.0001
Cytoplasm 0.082 0.1325 0.0649 0.1574 0.0648 0.1147 0.064 0.0629 0.0623 0.0826 0.0825 0.083 0.1096 0.2164 0.1502 0.1607 0.1518 0.1998
Cytoplasmic Foci 0.029 0.0071 0.0051 0.0086 0.0175 0.0093 0.0158 0.0046 0.0068 0.0039 0.0135 0.0123 0.0241 0.0069 0.0101 0.003 0.0245 0.019
Eisosomes 0.0003 0.0001 0.0004 0.0001 0.0001 0.0002 0.0003 0.0005 0.0002 0 0.0003 0.0001 0.0007 0.0002 0.0004 0.0001 0.0003 0.0002
Endoplasmic Reticulum 0.003 0.002 0.0012 0.0009 0.0012 0.0011 0.0022 0.0009 0.0014 0.0006 0.0009 0.0004 0.0021 0.0015 0.0021 0.001 0.0025 0.0017
Endosome 0.015 0.0019 0.0061 0.006 0.0028 0.0032 0.0251 0.0005 0.0077 0.0018 0.0386 0.003 0.0108 0.0027 0.0101 0.0008 0.0087 0.006
Golgi 0.0042 0.0002 0.0006 0.0028 0.0013 0.0013 0.0049 0.0001 0.0025 0.0011 0.0088 0.0015 0.0033 0.0016 0.0025 0.0001 0.0016 0.0024
Lipid Particles 0.0118 0.0004 0.0006 0.0069 0.0025 0.0027 0.0088 0.0005 0.0076 0.0353 0.0004 0.0038 0.0106 0.004 0.005 0.0001 0.0136 0.0042
Mitochondria 0.0051 0.0023 0.0014 0.0007 0.0012 0.0007 0.0075 0.001 0.0161 0.0025 0.0033 0.0004 0.0067 0.0015 0.003 0.0004 0.001 0.0035
None 0.6551 0.7498 0.7666 0.6096 0.6384 0.727 0.7821 0.8334 0.7806 0.623 0.7333 0.8181 0.6278 0.6377 0.6684 0.6719 0.5389 0.6071
Nuclear Periphery 0.0168 0.003 0.0038 0.0056 0.0054 0.0071 0.0037 0.0014 0.0029 0.0045 0.0036 0.001 0.0069 0.003 0.0062 0.0045 0.0088 0.0059
Nucleolus 0.0021 0.0013 0.002 0.0012 0.0014 0.0015 0.0008 0.0005 0.0008 0.0016 0.0004 0.0006 0.0066 0.001 0.0025 0.001 0.0051 0.0012
Nucleus 0.1038 0.0664 0.1083 0.1607 0.1945 0.0928 0.0361 0.0674 0.0784 0.2089 0.0868 0.0517 0.1162 0.098 0.1004 0.1421 0.1807 0.1101
Peroxisomes 0.0161 0.003 0.0006 0.0028 0.007 0.0021 0.0093 0.002 0.0136 0.0137 0.0005 0.0096 0.0125 0.0007 0.0035 0.0001 0.0109 0.0056
Punctate Nuclear 0.0259 0.0083 0.0212 0.0278 0.0108 0.0256 0.0096 0.0028 0.0043 0.0183 0.0052 0.0057 0.0222 0.0068 0.012 0.0128 0.0478 0.0195
Vacuole 0.0013 0.0011 0.0016 0.0003 0.001 0.0004 0.0021 0.0005 0.0013 0.0008 0.0065 0.0003 0.0042 0.0012 0.0027 0.0005 0.0007 0.0008
Vacuole Periphery 0.0012 0.0001 0.0005 0.0002 0.0003 0.0002 0.0005 0.0001 0.0007 0.0002 0.0015 0.0001 0.001 0.0003 0.0008 0.0001 0.0003 0.0004

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 11.7383 5.8296 14.2753 3.8082 10.5689 11.7318 9.5783 9.7468 12.356 2.9123
Translational Efficiency 1.4223 2.4804 0.9956 1.8397 1.2166 1.1239 2.0296 1.3128 1.4663 3.7795

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1269 1407 1043 240 1595 1871 154 234 2864 3278 1197 474

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 671.88 667.51 931.03 951.01 718.64 687.06 914.51 915.23 697.92 678.67 928.90 933.35
Standard Deviation 179.54 119.34 125.95 318.13 81.36 90.34 169.61 186.59 136.05 104.24 132.49 262.20
Intensity Change Log 2 — -0.009414 0.470624 0.501257 — -0.064833 0.347730 0.348865 — -0.037790 0.408419 0.424510

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000138 0.000246 0.000562 0.000880 0.000036 0.000508 0.000496 0.000431 0.000081 0.000396 0.000553 0.000658
Bud Neck 0.008002 0.033002 0.004909 0.009087 0.004842 0.036051 0.001253 0.003919 0.006242 0.034742 0.004438 0.006536
Bud Site 0.004581 0.008216 0.007059 0.028732 0.000956 0.010711 0.001944 0.010348 0.002562 0.009640 0.006401 0.019656
Cell Periphery 0.000456 0.000142 0.000135 0.000305 0.000103 0.000233 0.000179 0.000142 0.000260 0.000194 0.000141 0.000224
Cytoplasm 0.427562* 0.368211* 0.221931* 0.353471* 0.541104* 0.382937* 0.279533* 0.412121* 0.490795* 0.376616* 0.229342* 0.382425*
Cytoplasmic Foci 0.098945 0.216448* 0.005648 0.015778 0.113083 0.192572* 0.010611 0.031956 0.106819 0.202820* 0.006286 0.023765
Eisosomes 0.000105 0.000142 0.000042 0.000052 0.000078 0.000179 0.000074 0.000062 0.000090 0.000163 0.000046 0.000057
Endoplasmic Reticulum 0.001267 0.001012 0.006054 0.002655 0.000431 0.000726 0.006312 0.002902 0.000802 0.000849 0.006087 0.002777
Endosome 0.002841 0.022099 0.002282 0.004541 0.003450 0.022274 0.002885 0.008139 0.003180 0.022199 0.002360 0.006318
Golgi 0.000581 0.004079 0.000103 0.000135 0.000685 0.005273 0.000094 0.001981 0.000639 0.004760 0.000102 0.001047
Lipid Particles 0.006159 0.012514 0.001529 0.001842 0.004644 0.011455 0.002510 0.002141 0.005316 0.011909 0.001656 0.001989
Mitochondria 0.002464 0.013024 0.001013 0.001454 0.001144 0.011153 0.000336 0.000449 0.001729 0.011956 0.000926 0.000958
Mitotic Spindle 0.000860 0.005526 0.006386 0.025629 0.001445 0.007899 0.000554 0.010081 0.001186 0.006880 0.005635 0.017954
None 0.032578 0.012201 0.004906 0.009041 0.003711 0.006827 0.005614 0.006532 0.016501 0.009134 0.004997 0.007802
Nuclear Periphery 0.000668 0.000989 0.002171 0.002113 0.000557 0.000897 0.001404 0.001639 0.000606 0.000937 0.002072 0.001879
Nuclear Periphery Foci 0.001584 0.000570 0.001439 0.002118 0.000285 0.000350 0.003200 0.001485 0.000861 0.000445 0.001666 0.001805
Nucleolus 0.002579 0.005684 0.000657 0.000698 0.000824 0.004146 0.000496 0.000849 0.001602 0.004806 0.000637 0.000772
Nucleus 0.395690* 0.219156* 0.687706* 0.418466* 0.295803* 0.228929* 0.597183* 0.372270* 0.340062* 0.224735* 0.676060* 0.395660*
Peroxisomes 0.001568 0.017209 0.000495 0.000676 0.001691 0.013848 0.000315 0.000864 0.001636 0.015291 0.000472 0.000769
Vacuole 0.010623 0.057280 0.044733 0.121806 0.024842 0.060720 0.084841 0.124717 0.018542 0.059243 0.049893 0.123243
Vacuole Periphery 0.000748 0.002249 0.000240 0.000520 0.000284 0.002314 0.000167 0.006971 0.000490 0.002286 0.000230 0.003705

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -2.80 -6.66 -2.22 -1.68 0.34 -3.16 -6.40 -5.72 -0.04 1.78 -3.61 -8.67 -4.24 -1.14 0.99
Bud Neck -9.46 2.45 -0.10 8.72 -2.01 -14.79 3.69 -0.36 13.02 -2.17 -17.22 2.00 -0.47 15.28 -1.90
Bud Site -2.72 -0.99 -2.75 -2.04 -2.44 -8.85 -4.08 -4.02 0.69 -2.62 -8.34 -2.92 -4.13 -1.50 -2.81
Cell Periphery 8.71 8.70 3.68 -0.93 -0.93 -6.56 -4.58 -1.65 5.31 3.54 3.41 6.29 2.86 0.99 -0.34
Cytoplasm 5.72 23.37 9.25 5.71 -4.81 17.83 15.78 14.46 5.58 -2.33 16.58 37.17 17.37 8.11 -6.40
Cytoplasmic Foci -16.65 21.94 17.53 32.98 -3.88 -13.26 25.54 20.56 32.81 -2.02 -21.01 35.14 27.27 46.73 -5.16
Eisosomes -3.99 7.92 3.71 7.25 -3.61 -5.48 -1.73 -0.17 5.34 1.56 -6.50 6.89 2.50 7.54 -3.84
Endoplasmic Reticulum 1.51 -16.30 -7.34 -7.91 6.16 -3.26 -8.13 -7.10 -6.64 3.42 -0.41 -19.46 -10.26 -10.16 6.86
Endosome -14.37 0.72 -1.65 12.38 -2.06 -13.96 -0.53 -0.63 10.65 -0.30 -19.83 1.60 -1.35 16.11 -2.06
Golgi -6.82 5.38 4.57 7.75 -2.44 -5.01 1.48 0.28 5.16 -1.05 -7.29 2.49 1.02 7.93 -1.26
Lipid Particles -5.51 5.80 5.38 12.08 -1.01 -6.75 2.08 4.34 10.95 2.08 -8.66 7.21 6.91 16.07 -0.58
Mitochondria -7.58 2.50 2.83 9.63 0.89 -9.69 1.85 1.49 11.05 -0.93 -12.18 2.29 3.07 14.59 1.43
Mitotic Spindle -3.59 -3.91 -3.91 -3.16 -2.99 -4.05 -0.60 -3.29 -2.20 -3.15 -5.37 -3.60 -5.10 -3.79 -3.90
None 6.52 10.72 9.25 2.52 -2.87 -3.24 -0.47 -0.98 1.91 -0.48 4.87 9.63 7.82 2.99 -2.39
Nuclear Periphery -1.63 -14.50 -8.95 -7.49 -1.49 -4.46 -9.27 -8.84 -7.44 -2.44 -3.50 -20.48 -12.69 -10.72 -1.49
Nuclear Periphery Foci 1.72 -0.67 -1.41 -2.17 -1.23 -1.00 -5.16 -5.79 -5.65 1.65 1.57 -4.45 -2.85 -3.52 -1.21
Nucleolus -4.78 6.80 6.53 8.05 -0.22 -6.77 3.02 1.26 7.07 -0.84 -8.07 6.80 5.98 10.68 -0.15
Nucleus 16.02 -24.32 0.90 -7.89 15.19 8.33 -11.20 -1.93 -5.32 7.72 17.26 -35.53 -1.09 -9.31 20.46
Peroxisomes -10.63 4.60 2.84 11.26 -1.17 -11.60 5.40 4.98 13.13 -0.07 -15.69 6.44 5.36 17.12 -0.90
Vacuole -18.29 -15.71 -13.89 -11.12 -10.09 -13.91 -8.01 -14.82 -12.84 -5.74 -22.24 -15.80 -20.18 -16.91 -14.38
Vacuole Periphery -3.56 1.43 1.22 9.04 -0.88 -6.80 1.87 -0.94 0.47 -1.00 -7.15 1.62 -0.64 1.81 -1.03
Standard name
Human Ortholog
Description Minor isoform of pyruvate decarboxylase; decarboxylates pyruvate to acetaldehyde, involved in amino acid catabolism; transcription is glucose- and ethanol-dependent, and is strongly induced during sulfur limitation
Localization
Cell Percentages cytoplasm (37%), nucleus (6%), mixed (45%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Pdc6

Pdc6


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Pdc6-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available