Standard name
Human Ortholog
Description Cytosolic catalase T; has a role in protection from oxidative damage by hydrogen peroxide
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.98 0.97 0.98 0.99 0.9 0.94 0.9 0.91 0.82 0.78 0.66 0.73 1.0 0.98 0.99 0.96 0.98 0.98 0.85 0.85 0.74 0.94 0.91 0.86
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0.08 0.07 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0.05 0.05 0.12 0.06 0.2 0.21 0.44 0.27 0 0 0 0 0 0 0.05 0.05 0.14 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0.06 0 0 0 0 0.06 0 0.06 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 1 0 0 1 0 0 2 0 1 1 0 1 0 1 0 0 0 0 2 1 3 0 0 5
Bud 1 3 0 0 2 2 5 9 7 4 7 6 0 1 0 1 0 2 2 4 0 1 4 13
Bud Neck 4 3 2 4 9 8 6 10 7 1 2 3 0 1 0 1 0 2 0 3 2 0 0 1
Bud Site 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 – – – – – –
Cell Periphery 6 0 3 7 2 3 2 2 7 3 1 7 4 3 4 7 9 11 0 1 3 0 0 1
Cytoplasm 246 262 268 325 277 377 452 581 329 245 227 259 267 369 443 379 415 479 215 239 201 127 213 329
Endoplasmic Reticulum 1 4 1 0 2 2 1 2 3 0 0 1 0 2 0 24 32 32 0 0 0 0 2 2
Endosome 0 0 1 0 0 0 0 0 3 3 2 2 1 1 0 3 4 0 3 1 2 0 0 1
Golgi 0 0 1 0 0 0 0 3 1 0 0 0 0 0 0 0 0 1 0 4 1 0 0 0
Mitochondria 4 5 0 2 16 21 58 39 81 65 150 97 0 0 0 8 7 2 12 14 38 1 0 4
Nucleus 0 1 0 2 7 1 3 9 2 7 4 8 1 2 1 5 1 2 0 0 0 1 2 1
Nuclear Periphery 0 0 0 0 0 0 0 0 1 9 4 4 0 0 0 0 0 1 0 0 0 0 1 1
Nucleolus 0 1 0 0 0 0 0 1 1 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0
SpindlePole 0 0 0 1 3 3 11 8 7 2 2 5 0 0 0 2 1 2 1 2 1 0 0 3
Vac/Vac Membrane 1 2 2 0 17 3 6 14 8 19 12 23 0 2 6 6 6 6 4 5 9 1 1 3
Unique Cell Count 251 270 273 327 308 401 501 635 400 313 343 354 268 375 449 393 425 491 252 283 274 135 235 382
Labelled Cell Count 264 281 278 342 335 421 546 678 458 359 411 417 273 382 455 436 475 540 252 283 274 135 235 382


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.4 4.7 5.1 4.8 5.1 4.2 4.4 5.0 4.3 4.7 4.5 5.3 6.0 6.2 5.9 5.8 6.0 5.5 5.7 6.4 6.3
Std Deviation (1e-4) 0.5 1.6 1.1 1.1 1.6 1.4 1.7 1.7 2.3 2.2 2.5 3.0 1.7 2.2 2.2 1.4 1.5 1.5 2.2 2.4 2.3
Intensity Change (Log2) – – – -0.1 -0.02 -0.28 -0.24 -0.05 -0.26 -0.12 -0.18 0.05 0.22 0.26 0.21 0.18 0.23 0.1 0.15 0.32 0.29


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 1.0473 0.8253 1.0468 0.6317 0.5469 0.5965 1.6881 1.0953 1.1413 0.9715 1.1961 1.1724 0.933 0.6655 0.4247 1.3279 1.0834 0.3073
Actin 0.0212 0.0025 0.0077 0.0056 0.0062 0.0152 0.0527 0.001 0.0138 0.0213 0.0055 0.0023 0.017 0.003 0.0182 0.0201 0.0742 0.0031
Bud 0.0027 0.0071 0.0141 0.0028 0.0022 0.0034 0.002 0.0021 0.0087 0.0005 0.0089 0.0032 0.0087 0.0159 0.0063 0.0046 0.0537 0.001
Bud Neck 0.0069 0.0004 0.0025 0.0007 0.0185 0.0017 0.013 0.0008 0.0033 0.0005 0.0006 0.002 0.003 0.0009 0.0036 0.0141 0.0005 0.002
Bud Periphery 0.0032 0.0043 0.0123 0.0032 0.0026 0.0086 0.0041 0.0014 0.0068 0.0006 0.0096 0.0073 0.0057 0.008 0.0119 0.0122 0.0221 0.0021
Bud Site 0.0155 0.0148 0.0159 0.0036 0.0111 0.0026 0.0259 0.0157 0.0514 0.0007 0.0018 0.0005 0.0263 0.024 0.0274 0.028 0.002 0.0004
Cell Periphery 0.0043 0.0004 0.0006 0.0003 0.0004 0.0003 0.0015 0.0004 0.0017 0.0001 0.0004 0.0003 0.001 0.0008 0.0005 0.0081 0.0004 0.0002
Cytoplasm 0.1241 0.1601 0.2133 0.1899 0.1956 0.2041 0.1397 0.2577 0.2304 0.2774 0.2247 0.3561 0.1343 0.3536 0.3133 0.4106 0.3206 0.3752
Cytoplasmic Foci 0.0191 0.0065 0.0122 0.0137 0.0229 0.0107 0.037 0.0053 0.0106 0.0077 0.0253 0.0228 0.0731 0.0138 0.028 0.0445 0.0462 0.025
Eisosomes 0.0007 0.0001 0.0002 0.0002 0.0001 0.0002 0.0006 0.0002 0.0005 0.0002 0.0002 0.0001 0.0004 0.0002 0.0001 0.0007 0.0003 0.0001
Endoplasmic Reticulum 0.0078 0.0019 0.0018 0.001 0.0044 0.0025 0.0188 0.0018 0.002 0.0019 0.0087 0.0038 0.0049 0.0033 0.0023 0.0083 0.0103 0.0023
Endosome 0.0324 0.002 0.0088 0.0029 0.0125 0.0037 0.0508 0.0012 0.0034 0.0156 0.0786 0.0068 0.0585 0.0071 0.0064 0.0519 0.0121 0.0058
Golgi 0.0048 0.0002 0.0028 0.0005 0.0044 0.0007 0.0104 0.0002 0.0019 0.0139 0.0056 0.0007 0.012 0.0039 0.0015 0.0097 0.0027 0.0021
Lipid Particles 0.009 0.0003 0.0028 0.0008 0.0039 0.0017 0.0184 0.0004 0.0062 0.0021 0.0074 0.0007 0.0241 0.0018 0.0012 0.0118 0.0112 0.009
Mitochondria 0.0146 0.0006 0.0024 0.0009 0.0225 0.0022 0.0051 0.0004 0.0015 0.0013 0.0045 0.0008 0.0073 0.0017 0.0014 0.0095 0.005 0.0072
None 0.6745 0.7902 0.6821 0.7561 0.6673 0.7044 0.5103 0.7054 0.6396 0.6529 0.5548 0.5742 0.5573 0.5481 0.5565 0.3351 0.3531 0.5299
Nuclear Periphery 0.0178 0.001 0.002 0.0011 0.0029 0.0091 0.038 0.0007 0.0016 0.0006 0.029 0.0019 0.008 0.0011 0.0019 0.0034 0.0273 0.0058
Nucleolus 0.0032 0.0006 0.0009 0.0018 0.0025 0.002 0.0038 0.0002 0.0011 0.0001 0.001 0.0003 0.005 0.0019 0.0019 0.0014 0.0027 0.0006
Nucleus 0.0109 0.003 0.0056 0.0025 0.0051 0.0113 0.0249 0.0021 0.0042 0.0004 0.008 0.0028 0.0051 0.0024 0.0031 0.0022 0.0424 0.0061
Peroxisomes 0.007 0.0005 0.0038 0.0026 0.0019 0.0011 0.0144 0.0004 0.005 0.0007 0.0057 0.004 0.0187 0.0006 0.0044 0.0064 0.001 0.0128
Punctate Nuclear 0.0108 0.002 0.0046 0.0082 0.0077 0.0126 0.0175 0.0009 0.0042 0.0003 0.0142 0.0048 0.0175 0.0032 0.0075 0.0055 0.007 0.0073
Vacuole 0.0071 0.0013 0.003 0.0012 0.0039 0.0012 0.0083 0.0015 0.0017 0.0009 0.0039 0.0038 0.0105 0.0038 0.0021 0.0101 0.0028 0.0015
Vacuole Periphery 0.0025 0.0002 0.0007 0.0003 0.0014 0.0007 0.0028 0.0001 0.0003 0.0003 0.0018 0.0007 0.0018 0.0011 0.0006 0.0019 0.0024 0.0007

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 4.573 3.504 1.7334 5.3997 6.3526 11.0998 5.6096 3.3876 7.5932 5.6961
Translational Efficiency – – – – – – – – – –

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✔
Translational Efficiency –

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1966 982 195 459 1532 1507 156 1236 3498 2489 351 1695

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 591.95 599.07 865.77 838.84 668.00 675.53 856.52 776.46 625.26 645.36 861.66 793.35
Standard Deviation 63.14 102.13 93.15 1054.88 69.05 89.07 83.82 1006.12 75.84 101.56 89.24 1019.93
Intensity Change Log 2 — 0.017249 0.548508 0.502920 — 0.016172 0.358639 0.217064 — 0.016678 0.450962 0.358436

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000077 0.000146 0.000538 0.001967 0.000074 0.000740 0.000136 0.003563 0.000076 0.000506 0.000360 0.003131
Bud Neck 0.021994 0.021983 0.003909 0.015161 0.010066 0.032143 0.001064 0.010021 0.016770 0.028134 0.002645 0.011413
Bud Site 0.003357 0.005568 0.005078 0.043897 0.002229 0.019233 0.000936 0.039265 0.002863 0.013842 0.003237 0.040519
Cell Periphery 0.000205 0.000177 0.000209 0.000346 0.000128 0.000423 0.000213 0.000462 0.000171 0.000326 0.000211 0.000431
Cytoplasm 0.476911* 0.403984* 0.116753* 0.399949* 0.539848* 0.433600* 0.296278* 0.460268* 0.504475* 0.421915* 0.196542* 0.443934*
Cytoplasmic Foci 0.181563* 0.183992* 0.018222 0.029378 0.172613 0.244104* 0.040169 0.031631 0.177643 0.220388* 0.027976 0.031021
Eisosomes 0.000309 0.000161 0.000097 0.000057 0.000140 0.000155 0.000131 0.000060 0.000235 0.000158 0.000112 0.000059
Endoplasmic Reticulum 0.000767 0.000725 0.006416 0.005290 0.000312 0.000656 0.002406 0.001357 0.000568 0.000683 0.004634 0.002422
Endosome 0.006297 0.014089 0.005489 0.011502 0.007127 0.025402 0.003719 0.015153 0.006661 0.020939 0.004702 0.014164
Golgi 0.001887 0.001726 0.000125 0.005458 0.001297 0.005054 0.000068 0.004703 0.001629 0.003741 0.000100 0.004908
Lipid Particles 0.011314 0.012487 0.004623 0.003024 0.005585 0.009373 0.003541 0.001752 0.008805 0.010602 0.004142 0.002096
Mitochondria 0.005685 0.010499 0.002088 0.004074 0.002542 0.010473 0.000248 0.007495 0.004308 0.010483 0.001270 0.006568
Mitotic Spindle 0.000582 0.002152 0.001511 0.065596 0.002397 0.009929 0.000171 0.045870 0.001377 0.006861 0.000915 0.051212
None 0.007678 0.018773 0.004457 0.009645 0.002851 0.005257 0.004283 0.010923 0.005564 0.010590 0.004380 0.010577
Nuclear Periphery 0.000438 0.000868 0.002288 0.001835 0.000379 0.000867 0.001779 0.001117 0.000412 0.000867 0.002062 0.001312
Nuclear Periphery Foci 0.000261 0.000384 0.001148 0.002804 0.000246 0.000557 0.000954 0.000865 0.000255 0.000489 0.001062 0.001390
Nucleolus 0.001724 0.004505 0.002329 0.001385 0.000778 0.002148 0.001089 0.001081 0.001309 0.003078 0.001778 0.001163
Nucleus 0.233687* 0.263714* 0.771647* 0.252946* 0.215998* 0.106240 0.543252* 0.251668* 0.225940* 0.168370* 0.670138* 0.252014*
Peroxisomes 0.003148 0.011089 0.001979 0.003912 0.003908 0.017145 0.000388 0.002224 0.003481 0.014756 0.001272 0.002681
Vacuole 0.041465 0.041446 0.050714 0.134034 0.030993 0.074243 0.099015 0.105529 0.036879 0.061303 0.072181 0.113248
Vacuole Periphery 0.000652 0.001532 0.000380 0.007740 0.000488 0.002257 0.000159 0.004993 0.000580 0.001971 0.000282 0.005737

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -4.29 -6.73 -2.72 -2.55 -1.10 -1.93 -3.79 -2.19 -1.40 -2.02 -2.06 -7.39 -2.61 -1.92 -1.96
Bud Neck 0.08 9.93 5.92 4.97 -2.29 -9.26 7.90 0.87 9.84 -6.96 -6.18 12.32 6.26 11.00 -5.56
Bud Site -2.94 -0.90 -5.55 -4.98 -4.28 -8.23 1.42 -8.21 -1.62 -8.48 -8.46 -0.70 -9.72 -4.33 -8.00
Cell Periphery 1.82 -0.84 -0.87 -1.11 -0.66 -1.37 -3.06 -2.30 0.54 -1.49 -1.18 -2.05 -2.04 0.14 -1.40
Cytoplasm 7.42 30.61 9.58 3.82 -13.28 10.97 14.96 13.88 3.37 -7.26 11.93 28.90 15.10 4.37 -16.21
Cytoplasmic Foci -0.28 28.85 34.14 25.08 -1.61 -9.68 22.24 31.61 36.86 1.76 -8.00 36.19 46.64 44.10 -0.07
Eisosomes 3.76 7.18 9.27 2.74 3.37 -1.41 0.40 9.98 12.38 6.06 4.09 6.82 11.52 6.86 6.88
Endoplasmic Reticulum 0.11 -8.52 -3.33 -3.35 3.83 -2.79 -6.02 -12.76 -8.18 3.10 -1.35 -10.08 -7.20 -6.64 6.30
Endosome -5.37 0.92 0.70 5.11 -0.26 -11.15 3.82 -0.84 9.22 -3.65 -12.75 2.79 -0.92 9.82 -2.75
Golgi 0.56 6.49 -0.12 -0.33 -2.05 -5.66 3.96 -1.44 3.28 -3.67 -4.97 7.49 -1.04 2.41 -4.17
Lipid Particles -0.59 6.13 9.64 7.53 3.55 -4.06 3.65 8.85 9.00 4.48 -1.93 7.06 12.72 11.84 5.86
Mitochondria -2.65 3.03 3.71 5.34 -0.02 -5.67 3.75 -1.40 4.03 -4.11 -5.44 4.17 0.93 5.80 -2.72
Mitotic Spindle -2.16 -2.05 -5.92 -5.67 -5.60 -3.85 2.26 -7.50 -4.79 -8.23 -4.62 -0.31 -9.80 -7.56 -9.69
None -3.74 3.03 0.82 3.99 -1.50 -2.21 -0.47 -3.08 -1.09 -2.49 -3.54 2.52 -1.07 2.16 -2.75
Nuclear Periphery -5.45 -10.21 -9.76 -7.12 1.59 -1.40 -9.02 -19.67 -3.00 1.73 -2.19 -13.50 -20.55 -5.26 3.26
Nuclear Periphery Foci -1.72 -4.95 -3.65 -3.44 -1.23 -2.84 -4.47 -7.96 -2.94 1.81 -3.18 -6.68 -6.52 -4.73 0.79
Nucleolus -3.26 -0.44 3.20 4.65 3.48 -5.41 -0.44 0.39 5.35 0.64 -4.57 -0.52 3.57 6.64 3.72
Nucleus -3.50 -28.38 -3.14 -0.63 21.85 18.00 -12.68 -9.27 -22.66 8.80 10.47 -26.50 -9.24 -15.80 20.91
Peroxisomes -6.60 2.39 1.52 6.59 -0.46 -8.26 5.70 3.95 10.63 -4.00 -10.70 5.31 5.02 12.78 -1.05
Vacuole -1.13 -3.71 -16.45 -15.85 -11.79 -13.93 -8.50 -22.13 -14.72 -0.18 -11.02 -8.77 -26.85 -21.63 -7.28
Vacuole Periphery -4.14 3.09 -2.21 -1.35 -2.49 -6.00 3.71 -3.60 -0.39 -4.25 -7.11 4.74 -4.14 -1.24 -4.78
Standard name
Human Ortholog
Description Cytosolic catalase T; has a role in protection from oxidative damage by hydrogen peroxide
Localization
Cell Percentages cytoplasm (37%), nucleus (1%), mixed (41%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Ctt1

Ctt1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Ctt1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available