Standard name
Human Ortholog
Description Splicing factor that modulates turnover of branched RNAs by Dbr1p; interacts with spliceosomal components and branched RNA splicing products; enhances Dbr1p debranching in vitro; conserved protein with domain organization identical from yeast to human; N-terminal homology to Dbr1p N-terminus, but Dbr1p catalytic residues not conserved; relocalizes to the cytosol in response to hypoxia
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0.05 0 0 0.06 0.05 0 0 0 0.05 0.16 0.14 0.15 0.12 0.11 0.09 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.14 0 0.22 0.09 0.17 0.36 0.57 0.3 0.55 0.64 0.61 0.55 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0.95 0.95 0.91 0.93 0.91 0.89 0.74 0.8 0.78 0.82 0.79 0.71 0.82 0.85 0.82 0.83 0.85 0.87 0.92 0.94 0.86 0.91 0.77 0.62
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0.05 0.08 0.1 0.07 0 0 0 0 0 0 0 0 0 0 0 0.05
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.06 0.12 0.23
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 1 1 1 3 0 0 1 0 0 0 0 1 0 1 3 1 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 1 0 0 0 0 2 1 4 2 7 4 6 0 0 0 1 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 3 4 5 4 5 6 14 10 4 7 5 10 26 37 40 37 24 14 2 0 0 0 1 1
Endoplasmic Reticulum 1 1 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1 2 0 0 0 0 2 1
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 1 1 1 0 0 0 1 2
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1 4 0 0 1 1 0 0 1
Mitochondria 36 12 34 7 23 51 143 67 104 157 108 116 4 4 5 12 5 3 3 2 2 0 3 2
Nucleus 251 283 138 70 122 126 185 177 146 199 140 149 134 233 223 253 178 140 247 272 135 106 161 106
Nuclear Periphery 4 1 0 0 0 3 6 7 5 5 4 4 0 0 2 2 4 2 0 0 0 0 0 3
Nucleolus 1 2 1 0 1 2 9 7 10 20 18 14 0 3 2 4 1 4 0 0 1 1 7 8
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 2 1 0 0 0 0 0 0 0 0 0
SpindlePole 0 1 0 0 0 0 3 0 1 0 0 0 0 0 1 1 2 0 1 0 0 0 2 0
Vac/Vac Membrane 2 6 0 1 2 0 2 4 0 0 1 5 1 3 8 8 4 1 6 8 8 6 25 40
Unique Cell Count 264 298 152 75 134 141 249 220 188 244 177 211 163 274 271 303 210 161 270 290 158 118 210 171
Labelled Cell Count 299 310 178 82 153 191 364 277 275 395 280 305 165 282 283 322 225 167 270 290 158 118 210 171


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.3 5.9 5.1 5.2 5.0 4.4 3.6 4.4 4.0 3.8 3.7 4.0 6.5 6.3 6.4 7.6 9.2 9.6 6.4 6.0 6.9
Std Deviation (1e-4) 0.8 1.0 1.2 1.3 1.4 1.6 1.2 1.5 1.0 0.9 1.1 1.8 1.9 1.6 1.6 1.8 2.1 2.0 2.3 1.6 1.6
Intensity Change (Log2) – – – 0.04 -0.03 -0.2 -0.48 -0.19 -0.35 -0.42 -0.47 -0.33 0.35 0.3 0.33 0.59 0.86 0.92 0.33 0.25 0.44


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0.4 1.1 0.6 0 0 0 0.7 3.8 3.4 3.7 3.1 2.8 2.0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria -2.4 -1.1 0 0 0 0 0 0 0 -5.4 -7.3 -7.0 -6.1 -6.1 -5.6
Nucleus 0.7 0.1 -0.4 -4.0 -2.7 -3.2 -2.5 -2.9 -4.7 -2.2 -1.7 -2.4 -2.1 -1.7 -1.1
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 2.6326 4.2933 3.2316 2.9618 3.5045 3.198 3.0747 3.19 3.2566 3.0097 2.8933 3.2094 5.1601 4.5867 4.0116 3.5523 3.6893 4.2832
Actin 0.2158 0 0.0006 0 0 0.0001 0.0171 0 0.0123 0 0.0017 0.002 0.0012 0.0001 0.0024 0 0 0.0007
Bud 0.0012 0 0.0001 0 0 0.0002 0.0013 0 0.0001 0 0.0002 0.0001 0 0.0009 0.0004 0 0 0.0001
Bud Neck 0.0005 0 0.0002 0.0001 0.0001 0.0017 0.0007 0.0001 0.0006 0 0.0004 0.0011 0.0001 0.0002 0.0004 0.0002 0.0001 0.001
Bud Periphery 0.0025 0 0.0002 0 0 0.0007 0.0025 0 0.0003 0 0.0003 0.0002 0 0.0015 0.0006 0 0 0.0003
Bud Site 0.0043 0.0001 0.0001 0 0 0.0015 0.0009 0 0.001 0 0.003 0.0003 0.0001 0.0013 0.0011 0.0001 0 0.0002
Cell Periphery 0.0011 0 0.0001 0 0 0.0002 0.0003 0 0.0002 0 0.0001 0.0001 0 0.0002 0.0001 0 0 0.0001
Cytoplasm 0.0038 0.0022 0.0001 0.0005 0 0.0003 0.0029 0.0002 0.0018 0.0001 0.0021 0.0003 0.0087 0.0535 0.0035 0.0001 0 0.0005
Cytoplasmic Foci 0.0034 0 0.0003 0 0 0.0007 0.0049 0 0.0023 0 0.0037 0.0029 0.0182 0.0014 0.0113 0 0 0.0057
Eisosomes 0.006 0 0 0 0 0 0.0002 0 0.0001 0 0.0001 0 0 0.0001 0 0 0 0
Endoplasmic Reticulum 0.0224 0.0001 0 0.0003 0 0.0001 0.003 0 0.0024 0 0.0009 0.0002 0.0002 0.0004 0.0023 0 0 0.0001
Endosome 0.0158 0.0001 0.0017 0.0003 0 0.0034 0.016 0 0.0058 0 0.0104 0.0038 0.0023 0.0007 0.0277 0 0 0.001
Golgi 0.0158 0 0.0013 0 0 0.0003 0.0022 0 0.0022 0 0.0026 0.0013 0.0003 0.0001 0.0203 0 0 0.0006
Lipid Particles 0.0048 0 0.001 0 0 0.0006 0.0056 0 0.0086 0 0.0043 0.0104 0.0009 0.0007 0.0301 0 0 0.0091
Mitochondria 0.0094 0.0004 0.0249 0.0005 0.0001 0.0009 0.0071 0.0001 0.0029 0.0004 0.0201 0.0027 0.0001 0.0029 0.013 0.0003 0.0002 0.0008
None 0.0132 0.0001 0 0.0007 0 0.0001 0.0062 0 0.0014 0 0.0018 0.0018 0.0174 0.011 0.0003 0 0 0.0003
Nuclear Periphery 0.0076 0.0117 0.0005 0.0584 0.0002 0.0007 0.0177 0.0045 0.0086 0.0032 0.0048 0.0048 0.0026 0.0021 0.01 0.001 0.0003 0.0022
Nucleolus 0.0028 0.0047 0.0028 0.0057 0.0079 0.0318 0.0069 0.0082 0.0077 0.0034 0.0201 0.0168 0.0021 0.014 0.0034 0.0071 0.0133 0.0188
Nucleus 0.6648 0.9778 0.9645 0.93 0.9915 0.9473 0.8896 0.9862 0.9348 0.9924 0.9106 0.9419 0.8006 0.9017 0.8611 0.9908 0.9857 0.9497
Peroxisomes 0.0015 0 0.0005 0 0 0.0001 0.0022 0 0.0017 0 0.0073 0.003 0.0005 0.0002 0.004 0 0 0.0017
Punctate Nuclear 0.001 0.0002 0 0.0007 0.0001 0.0004 0.0048 0.0005 0.003 0.0002 0.004 0.0046 0.1444 0.0038 0.0028 0.0001 0.0001 0.0066
Vacuole 0.001 0.0015 0.0006 0.0006 0 0.0081 0.005 0.0001 0.0015 0 0.0012 0.0014 0.0001 0.0021 0.003 0.0001 0 0.0004
Vacuole Periphery 0.0013 0.0011 0.0003 0.002 0 0.0006 0.0027 0.0001 0.0006 0.0001 0.0004 0.0004 0 0.0012 0.0021 0.0001 0 0.0002

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 18.4625 21.3585 16.8095 19.3839 20.1825 22.4322 19.4688 22.0842 17.5222 20.247
Translational Efficiency 1.2992 1.2039 1.2426 0.9832 0.9545 0.8447 1.1677 0.9306 0.9586 0.9661

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1717 531 1884 2202 1527 1244 252 543 3244 1775 2136 2745

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 691.00 906.83 949.48 1025.93 803.71 811.12 1106.58 962.74 744.05 839.75 968.01 1013.43
Standard Deviation 84.72 350.55 128.02 147.91 101.66 131.74 120.93 152.89 108.76 225.49 136.93 151.02
Intensity Change Log 2 — 0.392146 0.458452 0.570175 — 0.013240 0.461361 0.260471 — 0.200822 0.460017 0.411938

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000062 0.000400 0.000349 0.000572 0.000071 0.000271 0.000879 0.001283 0.000066 0.000310 0.000412 0.000713
Bud Neck 0.001690 0.006058 0.009635 0.024164 0.001741 0.002706 0.003956 0.014308 0.001714 0.003709 0.008965 0.022214
Bud Site 0.000589 0.001164 0.001200 0.003397 0.000270 0.000724 0.000624 0.011912 0.000439 0.000856 0.001132 0.005082
Cell Periphery 0.000152 0.000184 0.000109 0.000112 0.000122 0.000147 0.000046 0.000235 0.000138 0.000158 0.000101 0.000136
Cytoplasm 0.009183 0.004004 0.002730 0.008635 0.002359 0.003489 0.000926 0.024582 0.005971 0.003643 0.002518 0.011790
Cytoplasmic Foci 0.000949 0.001733 0.000071 0.000561 0.000182 0.001475 0.000035 0.009005 0.000588 0.001552 0.000067 0.002231
Eisosomes 0.000009 0.000018 0.000023 0.000017 0.000010 0.000018 0.000038 0.000031 0.000009 0.000018 0.000025 0.000020
Endoplasmic Reticulum 0.002144 0.007613 0.004026 0.004977 0.003494 0.006152 0.008638 0.006005 0.002779 0.006589 0.004570 0.005180
Endosome 0.000591 0.000578 0.000192 0.001003 0.000369 0.000652 0.000175 0.010070 0.000487 0.000630 0.000190 0.002797
Golgi 0.000052 0.000260 0.000030 0.000336 0.000027 0.000969 0.000044 0.005612 0.000040 0.000757 0.000031 0.001380
Lipid Particles 0.000207 0.000292 0.000085 0.000125 0.000070 0.000828 0.000172 0.001438 0.000142 0.000668 0.000095 0.000384
Mitochondria 0.001838 0.002098 0.000702 0.001945 0.000886 0.002009 0.000712 0.004713 0.001389 0.002036 0.000704 0.002493
Mitotic Spindle 0.001170 0.002466 0.000644 0.013002 0.000061 0.000908 0.000187 0.025711 0.000648 0.001374 0.000590 0.015516
None 0.008434 0.003844 0.002472 0.003273 0.001526 0.003940 0.001286 0.017351 0.005182 0.003911 0.002332 0.006057
Nuclear Periphery 0.000352 0.000445 0.000418 0.001661 0.000385 0.000436 0.000383 0.003150 0.000367 0.000438 0.000414 0.001955
Nuclear Periphery Foci 0.000112 0.000122 0.000061 0.000205 0.000166 0.000200 0.000075 0.001332 0.000137 0.000177 0.000063 0.000428
Nucleolus 0.003633 0.004295 0.002985 0.001176 0.002190 0.003615 0.002883 0.003020 0.002954 0.003818 0.002973 0.001540
Nucleus 0.967750* 0.962075* 0.973221* 0.930288* 0.985015* 0.968857* 0.975170* 0.835507* 0.975877* 0.966828* 0.973451* 0.911539*
Peroxisomes 0.000254 0.000931 0.000101 0.000108 0.000057 0.001186 0.000159 0.000914 0.000161 0.001110 0.000108 0.000268
Vacuole 0.000703 0.001126 0.000861 0.003390 0.000793 0.001203 0.003539 0.014641 0.000745 0.001180 0.001177 0.005615
Vacuole Periphery 0.000127 0.000294 0.000085 0.001055 0.000207 0.000218 0.000073 0.009178 0.000165 0.000240 0.000083 0.002662

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -6.74 -13.49 -10.07 -3.30 -4.58 -6.31 -10.41 -8.28 -6.92 -1.00 -9.13 -16.62 -12.89 -7.90 -5.42
Bud Neck -3.80 -10.94 -16.29 -10.75 -10.55 -2.55 -6.82 -9.24 -8.50 -6.74 -4.24 -13.18 -18.43 -16.21 -11.36
Bud Site -1.35 -1.30 -5.31 -3.47 -3.34 -2.27 -4.08 -3.95 -3.71 -3.47 -2.03 -1.87 -6.86 -5.95 -4.58
Cell Periphery -0.35 1.25 1.56 0.87 0.65 -0.65 2.97 -1.43 -0.45 -4.67 -0.51 1.59 1.24 1.23 -0.69
Cytoplasm 2.19 5.28 0.85 -1.82 -6.28 -1.48 0.72 -7.08 -6.57 -7.28 2.19 4.50 -4.44 -6.28 -9.23
Cytoplasmic Foci -0.65 3.23 2.08 1.31 -1.42 -2.65 1.90 -2.84 -1.56 -2.95 -2.07 3.48 -1.28 1.14 -3.25
Eisosomes -5.61 -9.99 -12.23 -1.59 1.74 -4.91 -10.04 -11.09 -7.43 1.48 -7.00 -12.80 -16.58 -4.86 0.99
Endoplasmic Reticulum -7.71 -8.83 -13.63 2.74 -5.72 -7.18 -10.03 -6.86 -2.09 3.55 -11.66 -9.96 -13.89 1.41 -4.35
Endosome 0.07 1.47 -0.17 -0.22 -1.91 -0.91 0.58 -3.82 -3.49 -3.95 -0.55 1.67 -2.93 -2.26 -4.25
Golgi -1.56 1.03 -1.70 0.31 -1.92 -2.35 -3.29 -2.45 -1.40 -2.41 -2.52 0.18 -2.92 0.29 -2.93
Lipid Particles -0.91 2.54 1.41 1.64 -1.40 -2.39 -4.46 -2.64 -0.01 -1.92 -2.35 0.65 -2.21 1.68 -2.51
Mitochondria -0.21 1.33 -0.57 -0.20 -8.94 -1.40 -0.05 -2.86 -1.45 -3.93 -0.91 1.21 -2.05 -0.84 -9.37
Mitotic Spindle -0.68 0.54 -4.93 -3.26 -5.39 -1.82 -1.36 -5.04 -4.81 -3.65 -1.00 -0.81 -7.11 -6.42 -6.78
None 2.04 5.11 4.84 0.37 -1.09 -3.23 0.09 -4.26 -3.08 -4.25 1.31 3.95 0.66 -0.81 -3.49
Nuclear Periphery -1.94 -2.59 -12.09 -10.67 -11.16 -0.82 -3.35 -3.27 -3.19 -2.91 -1.75 -3.08 -10.62 -9.97 -9.81
Nuclear Periphery Foci -0.20 -0.84 -2.31 -1.95 0.46 -0.62 -0.22 -2.36 -1.81 -2.40 -0.79 -0.75 -2.44 -0.77 -0.13
Nucleolus -0.76 1.36 5.45 4.15 5.22 -2.94 -1.20 -1.59 0.62 0.15 -2.03 -0.36 3.87 5.31 4.16
Nucleus 1.11 -1.25 9.57 5.48 12.53 5.04 5.01 11.59 9.70 8.68 3.17 2.55 16.48 12.17 15.43
Peroxisomes -1.55 1.33 1.50 1.98 0.59 -2.61 -5.18 -3.23 1.48 -2.34 -2.84 0.69 -0.27 2.81 -1.51
Vacuole -2.30 -2.02 -8.26 -7.13 -7.55 -1.04 -5.44 -6.35 -6.20 -4.04 -1.74 -4.69 -9.97 -9.06 -8.47
Vacuole Periphery -1.14 1.43 -1.61 -0.95 -1.74 -0.11 1.32 -3.14 -3.13 -3.24 -1.01 1.76 -3.28 -3.04 -3.53
Standard name
Human Ortholog
Description Splicing factor that modulates turnover of branched RNAs by Dbr1p; interacts with spliceosomal components and branched RNA splicing products; enhances Dbr1p debranching in vitro; conserved protein with domain organization identical from yeast to human; N-terminal homology to Dbr1p N-terminus, but Dbr1p catalytic residues not conserved; relocalizes to the cytosol in response to hypoxia
Localization
Cell Percentages nucleus (98%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-4

Drn1

Drn1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Drn1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available