Standard name
Human Ortholog
Description Mitochondrial serine protease; required for the processing of various mitochondrial proteins and maintenance of mitochondrial DNA and morphology; belongs to the rhomboid-GlpG superfamily of intramembrane peptidases
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0.06 0 0.05 0.06 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.06 0.08 0 0 0 0 0 0
Cytoplasm 0.81 0.82 0.95 0.91 0.86 0.72 0.62 0.7 0.51 0.65 0.6 0.65 0.98 0.99 0.98 0.57 0.58 0.5 0.34 0.39 0.38 0.4 0.49 0.45
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.08 0.05 0.07 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.08 0.12 0.12 0 0 0.05 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.25 0.16 0 0.1 0.05 0.3 0.41 0.29 0.56 0.41 0.58 0.43 0 0 0 0.18 0.1 0.14 0.44 0.36 0.38 0.39 0.31 0.35
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0.08 0.07 0.06 0 0 0.06 0 0 0 0.05 0.08 0.08 0 0 0 0 0.05 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.08 0.12 0.1 0 0.1 0.05 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 1 0 0 1 0 0 0 0 0 0 0 0 0 0 0 2 1 1 5 3 1 3 2 5
Bud 1 2 0 1 2 1 12 7 10 11 13 10 2 1 0 3 3 3 0 3 0 1 5 2
Bud Neck 5 1 7 6 17 8 19 20 6 12 6 7 1 1 4 5 0 2 4 4 0 1 2 4
Bud Site 0 0 0 0 0 1 2 3 2 5 3 2 0 0 0 0 0 3 – – – – – –
Cell Periphery 0 0 3 0 2 1 1 1 1 0 0 2 4 2 1 8 10 9 2 1 3 1 4 2
Cytoplasm 227 115 159 142 241 183 221 227 148 248 157 209 169 244 222 93 90 59 99 72 69 45 116 107
Endoplasmic Reticulum 3 5 1 1 0 0 0 0 3 0 0 0 0 0 1 13 8 8 6 1 3 0 3 2
Endosome 1 2 1 0 5 2 1 0 0 1 0 0 0 0 1 13 19 14 11 5 8 1 2 3
Golgi 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 1 0 0 0
Mitochondria 69 22 2 16 13 75 146 94 160 155 153 138 1 0 0 29 16 17 129 66 68 44 73 82
Nucleus 1 0 0 0 4 2 2 4 2 3 2 3 0 0 0 4 1 1 0 0 0 0 0 0
Nuclear Periphery 1 0 1 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0
Nucleolus 0 1 0 0 2 0 1 0 1 1 0 0 0 0 0 0 1 0 0 0 0 0 0 0
Peroxisomes 0 0 1 2 1 1 0 0 0 1 0 0 3 0 1 12 3 0 2 0 0 0 0 1
SpindlePole 3 0 2 3 10 9 27 22 16 8 8 19 0 1 1 9 13 9 8 3 2 3 11 7
Vac/Vac Membrane 0 4 1 0 6 3 2 5 2 1 3 6 0 0 0 13 18 12 11 17 9 3 7 6
Unique Cell Count 281 141 168 156 280 254 359 322 288 380 262 321 173 247 227 164 155 118 296 186 182 114 238 237
Labelled Cell Count 312 152 179 172 303 286 435 383 351 446 345 396 180 249 231 204 183 139 296 186 182 114 238 237


Mitochondria

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 2.9 4.0 4.6 4.2 4.5 3.6 3.1 3.7 2.9 3.2 2.7 3.2 4.3 4.6 4.7 5.2 5.3 5.2 4.0 4.5 4.7
Std Deviation (1e-4) 0.5 0.6 1.6 1.8 1.4 1.1 1.0 1.1 1.0 1.0 0.7 0.8 0.7 1.1 1.3 1.2 1.4 1.8 1.3 1.2 1.6
Intensity Change (Log2) – – – -0.12 -0.04 -0.36 -0.55 -0.31 -0.64 -0.52 -0.74 -0.53 -0.11 -0.0 0.03 0.17 0.22 0.16 -0.21 -0.04 0.02


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – 0 0 0
Bud Neck – – – – – – – – – – – – 0 0 0
Bud Site – – – – – – – – – – – – 0 0 0
Cell Periphery – – – – – – – – – – – – 0 0 0
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – 0 0 0
Endosome – – – – – – – – – – – – 0 0 0
Golgi – – – – – – – – – – – – 0 0 0
Mitochondria – – – – – – – – – – – – 0 0 0
Nuclear Periphery – – – – – – – – – – – – 0 0 0
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – 0 0 0
Peroxisomes – – – – – – – – – – – – 0 0 0
SpindlePole – – – – – – – – – – – – 0 0 0
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – 0 0 0
Cytoplasm – – – – – – – – – – – – 0 0 0
Nucleus – – – – – – – – – – – – 0 0 0
Vacuole – – – – – – – – – – – – 3.3 4.2 3.8

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 0.2704 -0.0556 -0.0483 0.0574 -0.0532 -0.0834 -0.8172 -0.2024 -0.4528 -0.582 -0.867 -0.3057 -1.446 -1.3924 -1.3671 -1.0471 -1.2962 -1.2896
Actin 0.0137 0.0167 0.0124 0.0221 0.0088 0.0137 0.0077 0.0014 0.0029 0.0024 0.0009 0.0088 0.0202 0.0034 0.0169 0.0048 0.0432 0.0079
Bud 0.0036 0.0052 0.0053 0.0147 0.0014 0.0011 0.0006 0.0011 0.0014 0.0026 0.0011 0.0015 0.0022 0.0018 0.0021 0.0039 0.002 0.0025
Bud Neck 0.0062 0.005 0.009 0.0158 0.0023 0.0194 0.0055 0.0029 0.0116 0.0145 0.0021 0.0057 0.014 0.0026 0.0057 0.0436 0.003 0.0062
Bud Periphery 0.0085 0.0128 0.0097 0.025 0.0024 0.0026 0.0014 0.0024 0.0035 0.0078 0.0015 0.0032 0.0042 0.0043 0.0039 0.0179 0.0079 0.0081
Bud Site 0.0188 0.042 0.0245 0.0046 0.0019 0.0022 0.0075 0.0207 0.0184 0.0016 0.001 0.0011 0.0185 0.0343 0.0135 0.0032 0.0017 0.0013
Cell Periphery 0.0017 0.0011 0.0016 0.0008 0.0003 0.0009 0.0011 0.0017 0.0008 0.0006 0.0002 0.0006 0.001 0.0019 0.0012 0.0011 0.0003 0.0007
Cytoplasm 0.0846 0.1087 0.0983 0.0682 0.1261 0.1232 0.0832 0.1015 0.1224 0.1275 0.0687 0.1155 0.0983 0.1258 0.1809 0.1367 0.0514 0.1805
Cytoplasmic Foci 0.1353 0.1188 0.124 0.1296 0.1085 0.1178 0.1166 0.0962 0.1223 0.0829 0.1563 0.1223 0.1234 0.1274 0.0914 0.0809 0.154 0.1194
Eisosomes 0.0007 0.0007 0.0007 0.0006 0.0003 0.0007 0.0007 0.0004 0.0007 0.0004 0.0002 0.0004 0.0008 0.0015 0.001 0.0006 0.0007 0.0011
Endoplasmic Reticulum 0.0032 0.0028 0.0031 0.0017 0.003 0.0013 0.0039 0.0025 0.0024 0.0033 0.0007 0.0022 0.0061 0.0029 0.0055 0.0043 0.0025 0.0034
Endosome 0.1099 0.0521 0.0836 0.0987 0.1486 0.0371 0.0929 0.0487 0.0663 0.0351 0.0718 0.0629 0.1132 0.0853 0.0608 0.0647 0.0582 0.0601
Golgi 0.0283 0.0154 0.0205 0.0449 0.0602 0.0156 0.0143 0.0098 0.0147 0.0054 0.0232 0.0113 0.0225 0.0129 0.0142 0.0071 0.0276 0.016
Lipid Particles 0.0405 0.0078 0.0146 0.0138 0.021 0.0106 0.0269 0.0092 0.0099 0.0031 0.0111 0.0113 0.0193 0.0121 0.0157 0.0064 0.0088 0.0083
Mitochondria 0.0929 0.0882 0.0874 0.063 0.1034 0.1006 0.0388 0.0437 0.087 0.1219 0.0729 0.0659 0.068 0.0537 0.0922 0.045 0.0451 0.084
None 0.3632 0.4858 0.4481 0.4463 0.3769 0.4947 0.5498 0.6221 0.4878 0.5488 0.5231 0.5279 0.4309 0.4865 0.4496 0.542 0.4638 0.454
Nuclear Periphery 0.0065 0.0008 0.0028 0.0008 0.0012 0.0007 0.0025 0.0006 0.0018 0.0019 0.0003 0.0017 0.0033 0.0012 0.0039 0.0019 0.0023 0.001
Nucleolus 0.0125 0.0009 0.0021 0.001 0.0007 0.0008 0.0043 0.002 0.0025 0.0014 0.0007 0.0015 0.0023 0.0019 0.0026 0.001 0.0006 0.0008
Nucleus 0.0158 0.0014 0.0027 0.0022 0.0012 0.0011 0.0046 0.0026 0.0037 0.005 0.0006 0.0047 0.0047 0.0026 0.0027 0.0019 0.001 0.0021
Peroxisomes 0.0265 0.026 0.0292 0.0359 0.0187 0.0477 0.0182 0.0167 0.02 0.0156 0.0573 0.0342 0.0245 0.015 0.0202 0.0235 0.1195 0.0289
Punctate Nuclear 0.0056 0.002 0.0023 0.0015 0.0019 0.0029 0.007 0.0021 0.0063 0.0032 0.0027 0.0045 0.0081 0.0041 0.002 0.0022 0.0016 0.003
Vacuole 0.0185 0.0046 0.0151 0.0062 0.008 0.0042 0.0109 0.0099 0.011 0.0111 0.0025 0.0104 0.0119 0.0165 0.0114 0.0054 0.0033 0.0081
Vacuole Periphery 0.0037 0.0012 0.003 0.0025 0.0031 0.0011 0.0017 0.0017 0.0024 0.0039 0.0011 0.0027 0.0026 0.0026 0.0027 0.0019 0.0015 0.0025

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 9.5395 9.4753 12.6559 17.2361 11.6813 11.653 13.4126 16.812 17.2139 13.4681
Translational Efficiency 1.3396 1.2499 1.1291 0.8462 1.0629 1.5248 1.075 1.173 1.0586 1.428

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
2026 109 1327 1699 1508 1655 159 1259 3534 1764 1486 2958

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 684.72 762.06 780.86 742.14 615.46 714.60 850.61 806.68 655.17 717.53 788.32 769.61
Standard Deviation 77.35 88.90 93.40 104.66 84.03 91.54 89.71 104.71 87.27 92.10 95.48 109.43
Intensity Change Log 2 — 0.154390 0.189550 0.116177 — 0.215471 0.466833 0.390331 — 0.183626 0.327461 0.252458

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000172 0.001508 0.000235 0.001248 0.000290 0.000418 0.000131 0.001530 0.000223 0.000485 0.000224 0.001368
Bud Neck 0.038562 0.083734 0.005678 0.010582 0.029547 0.098992 0.001068 0.008405 0.034715 0.098049 0.005185 0.009655
Bud Site 0.007936 0.045921 0.010842 0.024239 0.010590 0.034265 0.000844 0.038728 0.009069 0.034985 0.009772 0.030406
Cell Periphery 0.000149 0.000258 0.000155 0.000173 0.000196 0.000258 0.000265 0.000219 0.000169 0.000258 0.000167 0.000193
Cytoplasm 0.387800* 0.189421* 0.360279* 0.471051* 0.402067* 0.262321* 0.275491* 0.472691* 0.393888* 0.257817* 0.351207* 0.471749*
Cytoplasmic Foci 0.283037* 0.257476* 0.016598 0.034610 0.256785* 0.251828* 0.076060 0.032095 0.271835* 0.252177* 0.022961 0.033540
Eisosomes 0.000208 0.000182 0.000037 0.000058 0.000205 0.000218 0.000183 0.000057 0.000207 0.000216 0.000053 0.000057
Endoplasmic Reticulum 0.000736 0.001429 0.002462 0.002309 0.002048 0.000801 0.001735 0.001954 0.001296 0.000840 0.002384 0.002158
Endosome 0.016327 0.057503 0.002476 0.005275 0.015972 0.051846 0.006131 0.007164 0.016176 0.052196 0.002867 0.006079
Golgi 0.004839 0.015375 0.000117 0.000920 0.004888 0.015893 0.000117 0.004021 0.004860 0.015861 0.000117 0.002240
Lipid Particles 0.011325 0.004771 0.001831 0.001508 0.016410 0.009460 0.006521 0.001565 0.013495 0.009170 0.002333 0.001532
Mitochondria 0.016760 0.050681 0.000719 0.002098 0.014701 0.052873 0.000222 0.003443 0.015881 0.052737 0.000666 0.002671
Mitotic Spindle 0.001852 0.006435 0.004097 0.030090 0.003800 0.009218 0.000056 0.019179 0.002683 0.009046 0.003665 0.025446
None 0.002824 0.000764 0.005383 0.005179 0.004770 0.002411 0.003280 0.003802 0.003655 0.002310 0.005158 0.004593
Nuclear Periphery 0.000453 0.000437 0.001827 0.001370 0.001221 0.000345 0.001313 0.001329 0.000781 0.000351 0.001772 0.001353
Nuclear Periphery Foci 0.000187 0.000508 0.000734 0.000800 0.000594 0.000286 0.001310 0.001065 0.000361 0.000300 0.000795 0.000913
Nucleolus 0.001211 0.002523 0.000832 0.000937 0.002338 0.003073 0.001660 0.000553 0.001692 0.003039 0.000920 0.000773
Nucleus 0.159649* 0.103126 0.548541* 0.300788* 0.173843* 0.090462 0.541302* 0.293041* 0.165706* 0.091245 0.547767* 0.297491*
Peroxisomes 0.011636 0.088981 0.000675 0.001432 0.015858 0.032206 0.000898 0.001948 0.013437 0.035714 0.000699 0.001652
Vacuole 0.052425 0.084948 0.036284 0.103299 0.041654 0.077718 0.081192 0.105156 0.047829 0.078165 0.041089 0.104089
Vacuole Periphery 0.001911 0.004020 0.000199 0.002035 0.002222 0.005107 0.000221 0.002054 0.002044 0.005039 0.000201 0.002043

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -3.03 -2.57 -2.21 1.40 -1.85 -1.39 1.22 -1.57 -1.25 -1.89 -4.11 -0.92 -2.68 -1.51 -2.50
Bud Neck -2.86 12.81 11.75 4.81 -2.96 -14.43 14.26 10.35 20.61 -8.73 -14.05 15.90 15.47 20.97 -3.10
Bud Site -4.84 -0.99 -4.56 3.64 -2.65 -9.67 8.31 -4.80 2.89 -8.77 -11.84 0.08 -6.61 5.82 -4.93
Cell Periphery -1.66 -0.36 1.07 1.89 1.27 -2.94 -3.04 1.40 4.22 4.10 -5.21 0.18 1.75 6.15 1.39
Cytoplasm 9.49 6.29 -1.73 -10.08 -7.36 14.76 6.57 2.72 -10.89 -5.04 17.83 8.65 0.46 -16.38 -7.85
Cytoplasmic Foci 1.30 51.29 48.46 11.31 -5.84 0.64 21.07 40.40 39.34 7.19 2.98 62.52 63.00 41.18 -1.91
Eisosomes 0.81 13.25 11.96 5.19 -5.08 -0.86 0.71 11.20 14.39 6.88 -0.75 16.04 16.28 15.51 -0.95
Endoplasmic Reticulum -2.24 -13.21 -11.19 -3.52 2.88 2.39 -0.26 -1.99 -11.02 -2.74 1.73 -7.37 -6.55 -11.49 2.18
Endosome -3.95 14.21 12.47 5.21 -2.37 -13.73 6.58 9.55 19.19 2.33 -14.77 17.72 15.65 20.46 -1.83
Golgi -3.36 10.03 8.73 4.86 -2.66 -7.88 8.46 3.88 10.08 -2.64 -8.69 13.12 8.51 11.97 -3.37
Lipid Particles 6.47 13.64 15.29 4.19 1.35 5.33 6.50 12.72 12.71 5.36 5.07 16.53 19.24 13.19 3.20
Mitochondria -2.38 10.90 10.26 3.53 -3.16 -11.03 10.81 9.10 15.91 -3.82 -11.25 14.90 13.64 16.44 -4.10
Mitotic Spindle -2.10 -3.03 -7.35 -4.63 -5.12 -3.54 3.25 -3.67 -1.23 -5.96 -5.40 -2.21 -8.16 -4.14 -5.57
None 3.68 -2.59 -2.37 -7.95 0.46 2.85 1.81 1.77 -2.01 -0.51 2.22 -1.35 -0.65 -3.51 0.97
Nuclear Periphery -0.01 -20.05 -15.22 -12.82 0.28 2.76 -0.83 -3.38 -13.52 -4.81 3.05 -8.91 -9.24 -20.75 -1.12
Nuclear Periphery Foci -2.74 -9.44 -10.92 -2.60 1.17 1.77 -3.40 -2.80 -5.76 1.54 0.22 -6.81 -6.97 -7.39 0.63
Nucleolus -1.21 0.81 1.54 1.48 0.34 -1.89 2.07 5.67 7.89 4.58 -4.10 2.40 5.34 7.14 1.45
Nucleus 3.20 -43.74 -22.55 -12.97 21.89 13.42 -15.25 -17.66 -28.57 8.02 15.41 -46.89 -28.58 -39.26 24.39
Peroxisomes -4.80 12.87 12.59 5.49 -1.85 -7.31 10.41 10.30 17.78 -0.07 -10.71 16.18 15.87 18.02 -1.66
Vacuole -3.14 -0.62 -20.84 -6.36 -19.14 -9.86 -5.74 -22.41 -16.39 -8.13 -9.24 -3.84 -30.47 -20.09 -24.43
Vacuole Periphery -2.45 10.02 2.05 3.20 -2.60 -6.21 6.65 3.02 8.90 -2.27 -8.01 11.41 3.49 9.66 -3.46
Standard name
Human Ortholog
Description Mitochondrial serine protease; required for the processing of various mitochondrial proteins and maintenance of mitochondrial DNA and morphology; belongs to the rhomboid-GlpG superfamily of intramembrane peptidases
Localization
Cell Percentages cytoplasm (17%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

Pcp1

Pcp1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Pcp1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available