Standard name
Human Ortholog
Description Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation; required for proper termination of transcription for some genes; involved in telomere maintenance
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0.08 0.05 0.07 0.09 0.07 0 0 0 0 0 0 0.09 0.14 0.12 0.1 0.08 0.09 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.09 0.1 0 0 0.28 0.14 0.14 0.11 0.65 0.32 0.46 0.4 0 0 0 0.09 0 0 0 0 0 0 0 0
Nucleus 0.86 0.82 0.85 0.83 0.74 0.8 0.81 0.84 0.67 0.83 0.7 0.75 0.84 0.83 0.78 0.82 0.82 0.81 0.61 0.7 0.69 0.63 0.59 0.58
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.2 0.07 0 0.05 0.07 0 0.07 0 0.1 0.06 0.1 0.09 0 0 0 0.09 0.08 0.05 0.13 0.08 0.11 0.2 0.2 0.17
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0.06 0 0 0 0.05 0.05 0 0 0 0 0 0 0 0 0 0.06 0.06 0 0 0.05 0 0.05
Vac/Vac Membrane 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0.07 0.08 0.05 0.08 0.07
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0
Bud 0 0 0 0 1 0 0 0 2 0 0 1 0 0 0 1 0 0 3 2 1 1 1 8
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 1 0 2 2
Bud Site 0 0 0 0 3 0 3 1 8 3 4 7 0 0 0 1 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 1 0 0 0 0 0 0 0
Cytoplasm 2 25 9 9 11 12 9 10 8 5 10 8 29 48 36 35 23 24 1 9 2 1 4 6
Endoplasmic Reticulum 0 1 0 0 0 0 0 0 0 0 1 0 0 0 0 6 6 13 0 0 0 0 0 0
Endosome 0 5 8 2 0 4 7 1 1 0 0 0 11 3 12 4 3 4 2 8 5 2 9 7
Golgi 0 0 0 0 0 1 0 0 0 0 0 0 0 0 1 0 1 0 2 5 1 0 2 2
Mitochondria 5 33 8 3 33 25 30 26 153 46 116 98 0 1 1 34 10 11 1 8 3 1 3 4
Nucleus 48 262 166 104 87 145 171 192 159 118 176 183 270 291 226 302 236 217 54 253 156 114 206 200
Nuclear Periphery 0 0 0 1 1 2 3 1 0 2 2 4 0 0 0 2 9 3 0 1 1 1 1 2
Nucleolus 11 22 2 6 8 8 14 7 23 9 24 22 10 11 11 34 23 13 11 30 25 35 68 59
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 1 0 2 0 0 0 0 1 0 0 0 0
SpindlePole 1 6 11 0 2 7 11 12 1 2 2 7 10 9 4 6 7 16 5 12 6 8 13 16
Vac/Vac Membrane 3 8 6 4 1 5 8 10 2 2 3 1 10 7 13 10 14 6 3 25 17 9 27 23
Unique Cell Count 56 321 196 125 118 181 211 229 237 143 251 245 323 350 290 367 287 267 89 364 227 181 351 344
Labelled Cell Count 70 362 210 129 147 209 256 260 357 187 338 333 341 371 306 435 333 307 89 364 227 181 351 344


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.5 5.1 6.0 5.6 4.5 5.0 5.0 5.3 4.0 4.4 4.4 4.5 6.4 6.6 6.2 5.5 7.2 6.7 6.3 6.8 6.8
Std Deviation (1e-4) 1.1 1.3 1.4 1.7 2.0 1.4 1.0 1.4 0.8 1.2 1.2 0.8 1.2 1.5 1.4 1.3 1.8 1.8 1.5 1.4 1.8
Intensity Change (Log2) – – – -0.08 -0.4 -0.26 -0.27 -0.19 -0.58 -0.43 -0.43 -0.41 0.1 0.13 0.04 -0.11 0.27 0.17 0.08 0.2 0.19

WT3RAP60RAP140RAP220RAP300RAP380RAP460RAP540RAP620RAP70002468WT3HU80HU120HU16002468WT3rpd3Δ_1rpd3Δ_2rpd3Δ_302468WT1AF100AF140AF18002468
rapamycinhydroxy-urearpd3 knockoutalpha-factorMultiple Condition Time-Course Intensity PlotsGFP Intensity (1e-4)GFP Intensity (1e-4)GFP Intensity (1e-4)GFP Intensity (1e-4)

Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 1.0 1.7 0.9 -0.2 -0.1 -0.6 -0.5 -0.3 -0.7 1.9 3.3 2.9 2.1 1.5 1.8
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 3.1
Endosome 0 0 -1.0 -0.4 0 0 0 0 0 -0.4 0 0 0 0 -1.7
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 3.3 3.5 2.8 0 0 0 0 0 0 0 2.2 -0.3 0
Nucleus -0.4 -2.4 -1.2 -1.0 -0.2 -4.2 -0.5 -3.6 -2.6 -0.3 -0.5 -1.9 -0.7 -0.7 -1.0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 2.9 0 0 0 0 0 0 0 1.9 3.8 3.4 2.3
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 -0.8 -0.2 -0.2 0 0 0 0 -1.4 -1.8 -2.6 -2.6 -1.8 0.2
Vacuole 0 0 -0.2 0.4 0.7 0 0 0 0 0 0 0.8 -0.2 1.0 -0.5

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration – – – – – – – – – – – – – – – – – –
Actin 0.7358 0.0005 0.0034 0.0001 0.0416 0.012 0.0321 0.0007 0.007 0.0002 0.0008 0.0046 – – – – – –
Bud 0.0026 0.0027 0.0002 0 0.0005 0.0023 0.0021 0.0016 0.0021 0.0001 0 0.0017 – – – – – –
Bud Neck 0.0023 0 0.0005 0 0.0014 0.0016 0.0359 0.0001 0.0013 0 0 0.0056 – – – – – –
Bud Periphery 0.0022 0.0056 0.0002 0 0.0007 0.0028 0.0054 0.0015 0.0044 0.0001 0 0.0034 – – – – – –
Bud Site 0.0126 0.0008 0.0015 0 0.0014 0.0004 0.0663 0.0036 0.0118 0.0001 0.0001 0.0003 – – – – – –
Cell Periphery 0.0005 0.0001 0.0001 0 0.0002 0.0001 0.0076 0.0001 0.0002 0 0 0.0003 – – – – – –
Cytoplasm 0.0076 0.0224 0.0183 0.0306 0.0097 0.0555 0.0154 0.0167 0.0367 0.0153 0.0448 0.0321 – – – – – –
Cytoplasmic Foci 0.0638 0.0029 0.0097 0.0015 0.0308 0.0037 0.1089 0.0017 0.0068 0.0022 0.0206 0.0031 – – – – – –
Eisosomes 0.0009 0.0001 0.0001 0.0001 0.0003 0.0002 0.0041 0.0003 0.0002 0.0001 0.0001 0.0001 – – – – – –
Endoplasmic Reticulum 0.0038 0.0004 0.0003 0.0003 0.0011 0.0006 0.0076 0.0002 0.0002 0.0002 0.0002 0.0009 – – – – – –
Endosome 0.0298 0.0034 0.0026 0.0002 0.0393 0.0048 0.0662 0.0001 0.0008 0.0002 0.0057 0.0003 – – – – – –
Golgi 0.076 0.0032 0.0051 0 0.0963 0.0101 0.0306 0 0.0005 0 0.0016 0.0001 – – – – – –
Lipid Particles 0.0157 0.0002 0.0012 0.0001 0.0865 0.0012 0.0158 0.0001 0.0011 0.0001 0.0084 0.0002 – – – – – –
Mitochondria 0.0115 0.0091 0.0022 0.0002 0.0165 0.0062 0.0101 0.0003 0.0005 0.0001 0.0006 0.0003 – – – – – –
None 0.0009 0.947 0.9304 0.9658 0.6571 0.8939 0.4753 0.9681 0.9177 0.9752 0.8815 0.9348 – – – – – –
Nuclear Periphery 0.0006 0.0002 0.0006 0.0002 0.0007 0.0008 0.0046 0.0002 0.0004 0.0003 0.0002 0.0011 – – – – – –
Nucleolus 0.0001 0.0001 0.001 0 0.0002 0.0001 0.0026 0.0003 0.0005 0.0003 0.0001 0.0003 – – – – – –
Nucleus 0.0003 0.0003 0.0032 0.0002 0.0002 0.0009 0.0026 0.0015 0.0013 0.0005 0.0004 0.0016 – – – – – –
Peroxisomes 0.0308 0.0003 0.0083 0.0001 0.0123 0.0007 0.0771 0.0002 0.0029 0.0002 0.0331 0.0003 – – – – – –
Punctate Nuclear 0.0014 0.0002 0.0109 0.0002 0.0013 0.0016 0.0193 0.0024 0.0034 0.0049 0.0014 0.0086 – – – – – –
Vacuole 0.0005 0.0003 0.0003 0.0001 0.001 0.0003 0.009 0.0001 0.0003 0.0001 0.0001 0.0002 – – – – – –
Vacuole Periphery 0.0003 0.0001 0.0001 0 0.0008 0.0002 0.0014 0 0.0001 0 0 0.0001 – – – – – –

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 12.5387 6.405 11.0899 13.5179 6.1931 8.3545 7.0158 14.2056 9.8869 15.11
Translational Efficiency 1.6135 1.9255 1.0856 0.5204 1.8359 1.8511 2.0366 0.764 1.4541 1.218

Hit Data

Dataset Hit
Protein Concentration –
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
149 32 139 1768 198 1638 1797 45 347 1670 1936 1813

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 675.24 771.38 667.10 683.24 640.89 779.92 704.83 1316.63 655.64 779.76 702.12 698.96
Standard Deviation 96.67 142.66 91.51 80.15 77.56 95.30 83.58 1933.63 87.95 96.44 84.73 329.82
Intensity Change Log 2 — 0.192041 -0.017497 0.016992 — 0.283249 0.137199 1.038701 — 0.237176 0.059905 0.603604

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000051 0.000600 0.000121 0.000156 0.000133 0.000261 0.000082 0.001252 0.000098 0.000268 0.000084 0.000183
Bud Neck 0.008193 0.020749 0.008435 0.004835 0.009383 0.025813 0.005804 0.018597 0.008872 0.025716 0.005993 0.005177
Bud Site 0.001013 0.043711 0.010401 0.006239 0.005750 0.019945 0.005383 0.075422 0.003716 0.020400 0.005743 0.007957
Cell Periphery 0.000136 0.000265 0.000211 0.000205 0.000194 0.000216 0.000159 0.008667 0.000169 0.000217 0.000163 0.000415
Cytoplasm 0.467691 0.384444 0.375467 0.368895 0.465311 0.527392 0.344182 0.353423 0.466333 0.524653 0.346428 0.368511
Cytoplasmic Foci 0.136940 0.034265 0.046401 0.020764 0.168137 0.172556 0.017830 0.070268 0.154741 0.169906 0.019881 0.021992
Eisosomes 0.000127 0.000068 0.000077 0.000039 0.000275 0.000253 0.000034 0.000082 0.000212 0.000250 0.000037 0.000040
Endoplasmic Reticulum 0.000431 0.002131 0.001366 0.001828 0.000401 0.000487 0.001027 0.000799 0.000414 0.000519 0.001052 0.001802
Endosome 0.006660 0.013683 0.004762 0.003539 0.005285 0.005121 0.002621 0.024706 0.005875 0.005285 0.002775 0.004064
Golgi 0.000343 0.001747 0.000118 0.000381 0.000515 0.001539 0.000254 0.019372 0.000441 0.001543 0.000244 0.000852
Lipid Particles 0.003478 0.015695 0.003336 0.001462 0.003177 0.001805 0.001318 0.008144 0.003307 0.002071 0.001463 0.001628
Mitochondria 0.001052 0.000850 0.000448 0.001761 0.004230 0.001073 0.000511 0.010128 0.002865 0.001068 0.000506 0.001969
Mitotic Spindle 0.000531 0.016539 0.001687 0.003738 0.000044 0.003029 0.005013 0.013095 0.000253 0.003288 0.004774 0.003970
None 0.002904 0.010651 0.016540 0.004298 0.001199 0.002485 0.006167 0.004256 0.001931 0.002641 0.006912 0.004297
Nuclear Periphery 0.000570 0.000850 0.001076 0.001785 0.000365 0.000177 0.001425 0.000905 0.000453 0.000190 0.001400 0.001763
Nuclear Periphery Foci 0.000241 0.001326 0.000539 0.000529 0.000196 0.000153 0.000260 0.001176 0.000215 0.000175 0.000280 0.000545
Nucleolus 0.000839 0.004120 0.001616 0.000879 0.000298 0.000446 0.000920 0.001369 0.000530 0.000517 0.000970 0.000891
Nucleus 0.272008 0.288553 0.482677 0.545656 0.238926 0.152997 0.584758 0.253726 0.253131 0.155594 0.577428 0.538410
Peroxisomes 0.006468 0.025181 0.000544 0.000636 0.002590 0.003573 0.000528 0.005164 0.004255 0.003987 0.000529 0.000748
Vacuole 0.090149 0.134238 0.043784 0.031800 0.093345 0.080333 0.021513 0.124134 0.091972 0.081366 0.023112 0.034092
Vacuole Periphery 0.000176 0.000334 0.000395 0.000576 0.000247 0.000345 0.000212 0.005315 0.000216 0.000345 0.000225 0.000693

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -3.53 -2.98 -3.24 2.93 -0.13 -2.18 1.09 -1.65 -1.38 -1.71 -3.07 -0.06 -2.37 1.41 -2.55
Bud Neck -0.72 0.02 1.02 1.17 0.69 -5.40 1.06 -0.73 1.28 -1.13 -6.82 1.19 1.51 12.24 0.55
Bud Site -1.95 -1.80 -4.20 1.63 0.86 -5.24 0.27 -1.64 -1.15 -1.66 -7.25 -1.25 -1.93 5.54 -0.89
Cell Periphery -1.56 -3.14 -2.23 0.32 0.59 -0.87 1.72 -1.26 -1.25 -1.27 -2.18 0.49 -1.43 -1.04 -1.50
Cytoplasm 1.76 3.06 4.41 0.07 -0.09 -3.69 6.20 2.47 4.35 -0.18 -4.34 7.81 6.43 18.60 -2.25
Cytoplasmic Foci 6.19 6.65 9.61 1.96 3.49 -0.52 11.77 4.35 5.51 -2.84 -1.86 14.97 14.69 33.34 -1.39
Eisosomes 2.62 2.75 6.64 2.28 4.83 0.43 6.47 4.49 7.54 -3.85 -1.55 7.81 7.65 14.19 -1.52
Endoplasmic Reticulum -2.88 -4.08 -6.71 0.19 -0.01 -0.18 -8.44 -2.35 -2.09 -0.26 -0.19 -9.45 -7.52 -5.61 -3.12
Endosome -0.57 0.83 1.98 1.34 1.33 0.05 1.80 -1.25 -1.26 -1.52 0.55 2.70 1.76 2.04 -1.33
Golgi -1.09 1.26 -0.01 1.10 -1.37 -2.13 0.95 -1.35 -1.24 -1.38 -2.45 0.98 -0.74 1.73 -1.38
Lipid Particles -0.88 -0.18 2.05 1.12 3.55 2.73 3.75 -1.61 -2.11 -2.25 2.43 3.48 3.25 0.91 -0.61
Mitochondria 0.62 0.99 -1.00 -2.36 -3.07 0.79 0.92 -0.42 -0.98 -1.07 0.77 1.01 0.43 -1.68 -3.08
Mitotic Spindle -1.25 -1.23 -4.05 0.79 -2.42 -2.78 -4.66 -1.15 -0.75 -0.24 -2.69 -4.53 -4.94 -1.10 1.16
None -1.20 -2.07 -1.41 0.87 1.86 -3.07 -7.67 -2.41 -1.19 1.99 -1.48 -6.25 -3.58 -2.62 3.19
Nuclear Periphery -0.79 -3.22 -7.62 -6.41 -4.05 4.31 -18.81 -4.34 -5.53 3.17 4.11 -13.68 -11.35 -14.91 -2.87
Nuclear Periphery Foci -3.36 -2.73 -1.89 2.41 0.46 1.98 -2.66 -2.69 -2.86 -2.34 2.40 -2.43 -2.56 -3.19 -1.62
Nucleolus -1.04 -2.42 -0.18 1.02 2.62 -2.83 -8.44 -1.98 -1.57 -0.43 0.02 -4.55 -2.62 -2.91 1.20
Nucleus 0.19 -6.11 -12.63 -5.22 -2.01 4.72 -17.91 -0.90 -3.07 7.29 6.78 -21.49 -18.52 -46.15 4.14
Peroxisomes -0.82 1.31 1.29 1.34 -0.44 -1.33 3.47 -0.53 -0.05 -1.51 0.08 1.94 1.85 5.33 -0.87
Vacuole -2.44 2.30 4.59 3.82 1.74 2.08 7.09 -1.77 -2.64 -4.44 2.41 8.75 6.66 9.46 -5.54
Vacuole Periphery -1.52 -1.67 -2.34 -1.67 -0.25 -0.85 0.20 -1.71 -1.66 -1.72 -1.68 -0.23 -2.37 -1.68 -2.45
Protein Complexes26S Proteasome complex37S mitochondrial small ribosomal subunit54S mitochondrial large ribosomal subunit60S cytosolic large ribosomal subunitAar2-U5 snRNPAnaphase-Promoting Complex variant 1Anaphase-Promoting Complex variant 2Anaphase-Promoting Complex variant 3Anaphase-Promoting Complex variant 4Arp2/3 complexATG1 kinase complexBLOC-1 complexCcr4-Not complexCentral kinetochore CTF19 complexCleavage and polyadenylation specificity factorcomplexCMG helicase complexCMG-Pol epsilon complexCOMPASS complexCOP9 signalosome COPI vesicle coat complexCoQ6 biosynthesis complexCore mediator complexCORVET complexCTF18-RFC complexCURI complex variant 1CURI complex variant 2CURI complex variant 3Cytoplasmic exosome complex, DIS3 variantDASH complexDNA-directed RNA Polymerase I complexDNA-directed RNA polymerase II complexDNA-directed RNA polymerase III complexDNA replication factor C complexEGO complexELG1-RFC complex Elongator holoenzyme complexEndoplasmic Reticulum Membrane ComplexEukaryotic translation initiation factor 2BcomplexEukaryotic translation initiation factor 3core complexExocystExomer complexFAR complexGeneral transcription factor complex TFIIDGeneral transcription factor complex TFIIHGET4-GET5 transmembrane domain recognition complexGID ubiquitin ligase complexGin4 complexGolgi transport complexGSE complexHMC complexHOPS complexINO80 chromatin remodeling complexLSM1-7 complexLsm1-7-Pat1 complexLSM2-7 complexLSM2-8 complexMCM complexMICOS complexMitochondrial electron transport complex IIIMitochondrial inner membrane pre-sequence translocasecomplexMitochondrial outer membrane translocase holocomplexMitochondrial proton-transporting ATP synthase complexMitochondrial pyruvate dehydrogenase complexNineTeen complexNOP8 60s ribosome pre-assembly complexNuA3 histone acetyltransferase complexNuA4 histone acetyltransferase complexNuclear condensin complexNuclear/nucleolar exosome complex, DIS3-RRP6 variantNuclear pore complexNucleolar ribonuclease MRP complexNucleolar ribonuclease P complexOligosaccharyl transferase complex variant 1Oligosaccharyl transferase complex variant 2PAF1 complexPAS complexPhosphatidylinositol 3-kinase complex IPolarisomePrefoldin complexPRP19-associated complexRad17 RFC-like complexRetromer complexRPD3L histone deacetylase complexRPD3S histone deacetylase complexRQC complexRSC complex variant RSC1RSC complex variant RSC2SAGA complexSCF-Met30 ubiquitin ligase complexSCF-Ufo1 ubiquitin ligase complexSEA complexSeptin complexSET3C histone deacetylase complexSignal recognition particleSLIK (SAGA-like) complexSmall ribosomal subunit processome, variant1Small ribosomal subunit processome, variant2Small ribosomal subunit processome, variant3SMC5-SMC6 SUMO ligase complexSm complexSpliceosomal commitment complexSPOTS complexSWI/SNF chromatin remodelling complexSwr1 chromatin remodelling complexTHO complexTranscription factor TFIIIC complexTranslocon complexTRAPPIII protein complexTRAPPII protein complexTRAPPI protein complexTREX complexU1 snRNPU2 snRNPU4 snRNPU4/U6 snRNPU4/U6.U5 tri-snRNP complexU5 snRNPU6 snRNP complexUTP-A complexUTP-B complexUTP-C complex variant 1UTP-C complex variant 2UTP-C complex variant 3Vacuolar proton translocating ATPase complex,Golgi variantVacuolar proton translocating ATPase complex,vacuole variantCellular Components••••••••••••••••actinbud neckcell peripherycytoplasmendosomeERGolgimitochondrionmixednuclear peripherynucleolusnucleusperoxisomespindle polevacuolar membranevacuole
Standard name
Human Ortholog
Description Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation; required for proper termination of transcription for some genes; involved in telomere maintenance
Localization
Cell Percentages nucleus (15%), cytoplasm (6%), mixed (46%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Srb5

Srb5


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Srb5-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available