Standard name | |
---|---|
Human Ortholog | |
Description | Subunit of the prohibitin complex (Phb1p-Phb2p); prohibitin is a 1.2 MDa ring-shaped inner mitochondrial membrane chaperone that stabilizes newly synthesized proteins; determinant of replicative life span; involved in mitochondrial segregation; prohibitin deficiency induces a mitochondrial unfolded protein response (mtUPR) |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 2 | 2 | 2 | 3 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 3 | 4 | 0 | 3 | 5 | 4 | 3 | 4 | 2 | 3 | 5 | 6 |
Bud | 1 | 12 | 4 | 4 | 1 | 2 | 2 | 9 | 11 | 6 | 10 | 5 | 1 | 0 | 1 | 10 | 3 | 2 | 2 | 2 | 0 | 3 | 6 | 3 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 5 | 1 | 3 | 5 | 7 |
Bud Site | 0 | 1 | 0 | 1 | 1 | 0 | 0 | 0 | 3 | 9 | 2 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 3 | 5 | 2 | 4 | 2 | 1 | 2 | 2 | 2 | 0 | 1 | 1 | 5 | 7 | 2 | 20 | 19 | 12 | 0 | 2 | 0 | 0 | 0 | 0 |
Cytoplasm | 3 | 40 | 16 | 10 | 7 | 17 | 13 | 5 | 10 | 6 | 8 | 4 | 22 | 62 | 108 | 34 | 21 | 34 | 0 | 11 | 1 | 10 | 27 | 29 |
Endoplasmic Reticulum | 4 | 5 | 1 | 2 | 4 | 2 | 1 | 2 | 3 | 2 | 1 | 2 | 4 | 7 | 3 | 10 | 6 | 5 | 0 | 0 | 1 | 1 | 4 | 4 |
Endosome | 2 | 11 | 32 | 15 | 5 | 30 | 16 | 26 | 2 | 1 | 2 | 0 | 23 | 29 | 45 | 35 | 16 | 14 | 13 | 28 | 14 | 16 | 27 | 26 |
Golgi | 2 | 1 | 10 | 11 | 4 | 3 | 1 | 2 | 0 | 0 | 1 | 1 | 12 | 5 | 0 | 63 | 74 | 87 | 8 | 5 | 5 | 4 | 11 | 11 |
Mitochondria | 108 | 238 | 70 | 122 | 119 | 140 | 168 | 170 | 175 | 193 | 159 | 129 | 24 | 33 | 24 | 19 | 13 | 23 | 102 | 228 | 141 | 101 | 138 | 131 |
Nucleus | 5 | 9 | 5 | 0 | 5 | 5 | 6 | 5 | 4 | 6 | 5 | 5 | 1 | 0 | 1 | 1 | 2 | 7 | 0 | 7 | 2 | 2 | 4 | 2 |
Nuclear Periphery | 3 | 1 | 1 | 0 | 4 | 4 | 11 | 5 | 8 | 14 | 4 | 5 | 0 | 0 | 0 | 3 | 0 | 1 | 2 | 3 | 1 | 1 | 5 | 6 |
Nucleolus | 8 | 1 | 1 | 0 | 5 | 13 | 9 | 9 | 5 | 13 | 10 | 5 | 0 | 0 | 1 | 5 | 6 | 15 | 2 | 0 | 1 | 2 | 6 | 1 |
Peroxisomes | 0 | 4 | 9 | 4 | 1 | 9 | 1 | 1 | 0 | 4 | 0 | 0 | 4 | 14 | 10 | 23 | 9 | 8 | 5 | 3 | 11 | 2 | 3 | 4 |
SpindlePole | 1 | 8 | 14 | 5 | 4 | 7 | 12 | 14 | 3 | 12 | 4 | 4 | 3 | 8 | 4 | 7 | 1 | 12 | 3 | 2 | 2 | 3 | 13 | 8 |
Vac/Vac Membrane | 32 | 69 | 26 | 19 | 9 | 37 | 27 | 46 | 5 | 11 | 9 | 4 | 25 | 24 | 24 | 63 | 41 | 56 | 9 | 41 | 7 | 17 | 35 | 33 |
Unique Cell Count | 135 | 316 | 150 | 157 | 145 | 201 | 225 | 237 | 194 | 211 | 181 | 141 | 104 | 159 | 201 | 244 | 180 | 230 | 159 | 355 | 203 | 183 | 308 | 284 |
Labelled Cell Count | 172 | 407 | 193 | 199 | 174 | 270 | 269 | 298 | 232 | 277 | 216 | 167 | 127 | 193 | 223 | 296 | 216 | 280 | 159 | 355 | 203 | 183 | 308 | 284 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 6.1 | 4.6 | 6.3 | 6.6 | 5.2 | 6.8 | 6.3 | 6.1 | 5.5 | 5.0 | 5.2 | 5.0 | 6.9 | 6.2 | 5.5 | 8.0 | 10.9 | 10.3 | 7.3 | 7.4 | 7.4 |
Std Deviation (1e-4) | 1.4 | 0.8 | 1.5 | 1.3 | 1.4 | 1.8 | 1.5 | 1.2 | 2.0 | 1.2 | 1.3 | 1.2 | 1.6 | 1.2 | 1.4 | 2.2 | 3.3 | 2.6 | 2.3 | 2.3 | 2.0 |
Intensity Change (Log2) | – | – | – | 0.06 | -0.27 | 0.09 | -0.01 | -0.05 | -0.22 | -0.34 | -0.29 | -0.34 | 0.12 | -0.02 | -0.21 | 0.33 | 0.79 | 0.69 | 0.2 | 0.22 | 0.22 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Bud Neck | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Bud Site | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Cell Periphery | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Cyto | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endoplasmic Reticulum | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Endosome | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Golgi | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Mitochondria | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Nuclear Periphery | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Nuc | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleolus | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Peroxisomes | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
SpindlePole | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Vac | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cortical Patches | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Cytoplasm | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Nucleus | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Vacuole | – | – | – | – | – | – | – | – | – | – | – | – | 2.0 | 1.2 | 1.6 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 5.3451 | 6.3504 | 5.7069 | 5.3095 | 4.9854 | 6.0935 | 9.0715 | 9.4193 | 8.6084 | 9.2502 | 9.4172 | 9.644 | 7.3049 | 6.8528 | 7.3156 | 6.3687 | 6.5053 | 7.0252 |
Actin | 0.0298 | 0.0392 | 0.0267 | 0.0056 | 0.0563 | 0.0256 | 0.051 | 0.0408 | 0.0456 | 0.0251 | 0.1203 | 0.0822 | 0.0115 | 0.0054 | 0.0297 | 0.0303 | 0.0167 | 0.0124 |
Bud | 0.0007 | 0.0028 | 0.0021 | 0.002 | 0.0009 | 0.0005 | 0.0046 | 0.0297 | 0.0025 | 0.0004 | 0.0307 | 0.0014 | 0.0013 | 0.0067 | 0.0034 | 0.0074 | 0.0049 | 0.0017 |
Bud Neck | 0.0017 | 0.0007 | 0.0025 | 0.002 | 0.0005 | 0.0194 | 0.0165 | 0.007 | 0.0025 | 0.0002 | 0.0002 | 0.0075 | 0.0014 | 0.0025 | 0.0017 | 0.0017 | 0.0069 | 0.004 |
Bud Periphery | 0.0014 | 0.0032 | 0.0034 | 0.0099 | 0.0011 | 0.0009 | 0.0152 | 0.0271 | 0.0039 | 0.0009 | 0.0871 | 0.0024 | 0.0024 | 0.0083 | 0.0045 | 0.0291 | 0.009 | 0.003 |
Bud Site | 0.0078 | 0.0087 | 0.0129 | 0.0035 | 0.0016 | 0.0012 | 0.0178 | 0.1966 | 0.0159 | 0.0005 | 0.0045 | 0.0019 | 0.003 | 0.0472 | 0.0102 | 0.0088 | 0.0099 | 0.0015 |
Cell Periphery | 0.0014 | 0.0005 | 0.0012 | 0.0037 | 0.0002 | 0.0004 | 0.0038 | 0.0023 | 0.0007 | 0.0002 | 0.0003 | 0.0005 | 0.0006 | 0.002 | 0.0009 | 0.0025 | 0.0006 | 0.0006 |
Cytoplasm | 0.002 | 0.0068 | 0.0016 | 0.0004 | 0.0014 | 0.0006 | 0.0144 | 0.0023 | 0.0138 | 0.0001 | 0.0006 | 0.0017 | 0.0073 | 0.0023 | 0.0016 | 0.0011 | 0.0018 | 0.0011 |
Cytoplasmic Foci | 0.0451 | 0.0403 | 0.0315 | 0.0075 | 0.0447 | 0.019 | 0.0488 | 0.052 | 0.0396 | 0.0048 | 0.0007 | 0.0323 | 0.0512 | 0.0324 | 0.0224 | 0.0216 | 0.0433 | 0.0252 |
Eisosomes | 0.0007 | 0.0004 | 0.0005 | 0.0002 | 0.0001 | 0.0002 | 0.0006 | 0.0004 | 0.0003 | 0.0004 | 0.0002 | 0.0005 | 0.0003 | 0.0002 | 0.0003 | 0.0002 | 0.0002 | 0.0002 |
Endoplasmic Reticulum | 0.0022 | 0.0016 | 0.0007 | 0.0004 | 0.0002 | 0.0005 | 0.0058 | 0.0005 | 0.0012 | 0.0001 | 0.0005 | 0.0007 | 0.0021 | 0.0006 | 0.0036 | 0.0006 | 0.001 | 0.0007 |
Endosome | 0.0726 | 0.0902 | 0.0649 | 0.0347 | 0.0631 | 0.048 | 0.125 | 0.0826 | 0.0463 | 0.0116 | 0.0019 | 0.0732 | 0.1075 | 0.1064 | 0.0558 | 0.0809 | 0.0799 | 0.065 |
Golgi | 0.0823 | 0.1189 | 0.0747 | 0.0499 | 0.0823 | 0.1309 | 0.0706 | 0.1112 | 0.0758 | 0.0278 | 0.0031 | 0.1855 | 0.1106 | 0.0911 | 0.0831 | 0.0993 | 0.0864 | 0.1403 |
Lipid Particles | 0.0538 | 0.0045 | 0.0162 | 0.0055 | 0.0081 | 0.0212 | 0.0303 | 0.0374 | 0.0223 | 0.0168 | 0.0005 | 0.0395 | 0.034 | 0.0183 | 0.0144 | 0.0124 | 0.0183 | 0.0247 |
Mitochondria | 0.5967 | 0.6071 | 0.6984 | 0.8304 | 0.6792 | 0.6895 | 0.3747 | 0.3315 | 0.6384 | 0.8879 | 0.7455 | 0.4948 | 0.5847 | 0.5774 | 0.6888 | 0.631 | 0.6387 | 0.6592 |
None | 0.0027 | 0.0003 | 0.0011 | 0.0002 | 0.0001 | 0.0001 | 0.0439 | 0.0004 | 0.0003 | 0.0001 | 0.0002 | 0.0003 | 0.0038 | 0.0002 | 0.0003 | 0.0004 | 0.002 | 0.0004 |
Nuclear Periphery | 0.0021 | 0.0004 | 0.0005 | 0.0001 | 0.0001 | 0.0003 | 0.0306 | 0.0002 | 0.0037 | 0.0001 | 0.0006 | 0.0008 | 0.0012 | 0.0003 | 0.0005 | 0.0006 | 0.0043 | 0.0042 |
Nucleolus | 0.0093 | 0.0004 | 0.0011 | 0.0018 | 0.0002 | 0.0006 | 0.0059 | 0.0032 | 0.0009 | 0.0002 | 0.0001 | 0.0004 | 0.0007 | 0.0142 | 0.0102 | 0.001 | 0.0031 | 0.0033 |
Nucleus | 0.002 | 0.0006 | 0.0006 | 0.0008 | 0.0002 | 0.0002 | 0.0148 | 0.0012 | 0.0068 | 0.0002 | 0.0002 | 0.0003 | 0.0007 | 0.0019 | 0.0013 | 0.0006 | 0.0018 | 0.0015 |
Peroxisomes | 0.0588 | 0.0475 | 0.0353 | 0.0175 | 0.0508 | 0.0325 | 0.0351 | 0.0473 | 0.0682 | 0.0171 | 0.0009 | 0.065 | 0.0438 | 0.029 | 0.0396 | 0.0402 | 0.0439 | 0.0309 |
Punctate Nuclear | 0.0073 | 0.0002 | 0.0008 | 0.0001 | 0.0002 | 0.0001 | 0.0128 | 0.0004 | 0.0006 | 0 | 0.0002 | 0.0004 | 0.0011 | 0.0003 | 0.0003 | 0.0003 | 0.0058 | 0.0008 |
Vacuole | 0.01 | 0.0184 | 0.0139 | 0.0169 | 0.004 | 0.0035 | 0.0433 | 0.0195 | 0.0068 | 0.0026 | 0.0007 | 0.0041 | 0.0166 | 0.0428 | 0.0208 | 0.0196 | 0.017 | 0.0119 |
Vacuole Periphery | 0.0096 | 0.0075 | 0.0095 | 0.0071 | 0.0045 | 0.0047 | 0.0346 | 0.0064 | 0.0039 | 0.0031 | 0.0011 | 0.0045 | 0.0142 | 0.0107 | 0.0066 | 0.0102 | 0.0046 | 0.0075 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 97.0587 | 94.756 | 94.8803 | 103.2042 | 92.7249 | 91.8995 | 111.9681 | 128.938 | 139.6926 | 112.5043 |
Translational Efficiency | 1.5724 | 1.5249 | 1.5354 | 1.6135 | 1.4134 | 1.8309 | 1.6174 | 1.6427 | 1.4951 | 1.3562 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✔ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
122 | 337 | 124 | 1583 | 2025 | 2447 | 2768 | 1692 | 2147 | 2784 | 2892 | 3275 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 667.81 | 912.03 | 1161.65 | 1081.83 | 784.76 | 938.84 | 1121.44 | 1099.25 | 778.11 | 935.59 | 1123.16 | 1090.83 |
Standard Deviation | 84.97 | 142.48 | 184.16 | 176.12 | 99.84 | 128.33 | 183.50 | 172.61 | 102.69 | 130.42 | 183.71 | 174.54 |
Intensity Change Log 2 | — | 0.449644 | 0.798666 | 0.695964 | — | 0.258628 | 0.515029 | 0.486196 | — | 0.349596 | 0.652380 | 0.586435 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.000494 | 0.003400 | 0.020714 | 0.005065 | 0.000414 | 0.000672 | 0.001603 | 0.003884 | 0.000419 | 0.001002 | 0.002423 | 0.004455 |
Bud Neck | 0.027831 | 0.008727 | 0.017173 | 0.009428 | 0.004710 | 0.004527 | 0.005790 | 0.004494 | 0.006024 | 0.005035 | 0.006278 | 0.006879 |
Bud Site | 0.018349 | 0.050795 | 0.039128 | 0.123380* | 0.011589 | 0.014327 | 0.050532 | 0.077797 | 0.011973 | 0.018741 | 0.050043 | 0.099830 |
Cell Periphery | 0.000953 | 0.000709 | 0.000946 | 0.000559 | 0.000299 | 0.000324 | 0.000530 | 0.000482 | 0.000336 | 0.000371 | 0.000548 | 0.000519 |
Cytoplasm | 0.005599 | 0.008152 | 0.003122 | 0.032467 | 0.004047 | 0.003170 | 0.009844 | 0.023003 | 0.004135 | 0.003773 | 0.009556 | 0.027577 |
Cytoplasmic Foci | 0.038088 | 0.073847 | 0.096754 | 0.060270 | 0.034269 | 0.025478 | 0.022000 | 0.055248 | 0.034486 | 0.031333 | 0.025205 | 0.057675 |
Eisosomes | 0.000103 | 0.000110 | 0.000148 | 0.000062 | 0.000059 | 0.000058 | 0.000056 | 0.000058 | 0.000061 | 0.000064 | 0.000060 | 0.000060 |
Endoplasmic Reticulum | 0.000439 | 0.000846 | 0.002787 | 0.001204 | 0.001080 | 0.000361 | 0.000667 | 0.001157 | 0.001043 | 0.000420 | 0.000758 | 0.001180 |
Endosome | 0.048365 | 0.111327 | 0.152867 | 0.078966 | 0.084414 | 0.078650 | 0.066634 | 0.081854 | 0.082366 | 0.082605 | 0.070332 | 0.080458 |
Golgi | 0.045548 | 0.135837* | 0.106806 | 0.109403 | 0.097786 | 0.084534 | 0.078965 | 0.134244* | 0.094818 | 0.090744 | 0.080159 | 0.122237* |
Lipid Particles | 0.002321 | 0.002393 | 0.003854 | 0.000560 | 0.003562 | 0.001421 | 0.000366 | 0.000543 | 0.003491 | 0.001538 | 0.000516 | 0.000551 |
Mitochondria | 0.714978* | 0.459980* | 0.238725* | 0.211790* | 0.655452* | 0.706245* | 0.510358* | 0.309843* | 0.658835* | 0.676435* | 0.498711* | 0.262448* |
Mitotic Spindle | 0.005652 | 0.037115 | 0.138571* | 0.190741* | 0.009433 | 0.010562 | 0.065853 | 0.112056* | 0.009218 | 0.013776 | 0.068971 | 0.150089* |
None | 0.000560 | 0.000301 | 0.000304 | 0.000538 | 0.000366 | 0.000751 | 0.000752 | 0.000539 | 0.000377 | 0.000697 | 0.000733 | 0.000538 |
Nuclear Periphery | 0.000053 | 0.000157 | 0.001741 | 0.000367 | 0.000383 | 0.000105 | 0.000705 | 0.000390 | 0.000364 | 0.000112 | 0.000749 | 0.000379 |
Nuclear Periphery Foci | 0.000085 | 0.000217 | 0.003427 | 0.000807 | 0.000400 | 0.000393 | 0.000748 | 0.001409 | 0.000382 | 0.000371 | 0.000862 | 0.001118 |
Nucleolus | 0.001388 | 0.000270 | 0.003316 | 0.000418 | 0.000856 | 0.000398 | 0.000866 | 0.000308 | 0.000886 | 0.000382 | 0.000971 | 0.000361 |
Nucleus | 0.002282 | 0.000696 | 0.004563 | 0.001024 | 0.001294 | 0.000893 | 0.000971 | 0.000819 | 0.001350 | 0.000869 | 0.001125 | 0.000918 |
Peroxisomes | 0.049507 | 0.055037 | 0.072406 | 0.013590 | 0.012296 | 0.014414 | 0.005700 | 0.008898 | 0.014410 | 0.019331 | 0.008560 | 0.011166 |
Vacuole | 0.017982 | 0.028679 | 0.053816 | 0.105370 | 0.036103 | 0.029230 | 0.098334 | 0.105443 | 0.035074 | 0.029163 | 0.096425 | 0.105408 |
Vacuole Periphery | 0.019422 | 0.021404 | 0.038833 | 0.053992 | 0.041188 | 0.023488 | 0.078726 | 0.077534 | 0.039951 | 0.023236 | 0.077016 | 0.066155 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -2.58 | -2.61 | -5.10 | -1.17 | 2.01 | -1.61 | -5.60 | -4.33 | -3.98 | -2.78 | -2.94 | -5.10 | -6.78 | -5.61 | -2.87 |
Bud Neck | 2.10 | 0.98 | 2.01 | -0.63 | 2.54 | 0.25 | -1.73 | 0.01 | -0.30 | 2.36 | 1.13 | -0.49 | -1.38 | -3.76 | -1.37 |
Bud Site | -3.99 | -2.82 | -17.25 | -8.74 | -10.42 | -1.68 | -15.94 | -18.23 | -17.40 | -6.67 | -3.88 | -16.15 | -28.58 | -25.53 | -13.89 |
Cell Periphery | 0.91 | 0.02 | 1.61 | 0.93 | 0.80 | -0.88 | -6.35 | -3.17 | -2.75 | 0.83 | -1.01 | -4.97 | -2.71 | -2.17 | 0.53 |
Cytoplasm | -0.90 | 0.94 | -9.49 | -10.89 | -15.89 | 1.33 | -7.00 | -12.48 | -13.33 | -8.29 | 0.52 | -6.79 | -19.10 | -20.00 | -13.95 |
Cytoplasmic Foci | -3.69 | -4.66 | -2.60 | 2.34 | 3.69 | 4.93 | 7.47 | -8.18 | -12.16 | -14.17 | 1.69 | 5.52 | -10.87 | -12.78 | -17.03 |
Eisosomes | -0.29 | -2.08 | 3.03 | 2.80 | 5.12 | 0.15 | 0.84 | 0.34 | 0.14 | -0.54 | -0.75 | 0.39 | 0.56 | 1.28 | 0.15 |
Endoplasmic Reticulum | -1.88 | -4.31 | -4.23 | -1.78 | 2.94 | 2.49 | 1.34 | -0.31 | -5.79 | -3.19 | 2.28 | 0.94 | -0.56 | -7.72 | -3.51 |
Endosome | -5.74 | -7.22 | -3.75 | 3.94 | 5.90 | 1.46 | 4.97 | 0.78 | -0.75 | -4.57 | -0.05 | 3.49 | 0.82 | 0.96 | -3.47 |
Golgi | -7.29 | -4.03 | -6.77 | 2.88 | -0.02 | 3.00 | 4.40 | -6.17 | -9.06 | -10.30 | 0.97 | 3.58 | -5.55 | -7.01 | -9.84 |
Lipid Particles | -0.06 | -1.58 | 2.70 | 1.95 | 4.21 | 2.46 | 4.12 | 3.79 | 2.76 | -2.18 | 2.38 | 4.05 | 3.92 | 3.32 | -1.23 |
Mitochondria | 7.37 | 12.34 | 17.48 | 12.00 | 1.13 | -5.41 | 15.15 | 33.48* | 40.03* | 19.54* | -1.89 | 17.09 | 46.71* | 51.25* | 27.99* |
Mitotic Spindle | -5.53 | -7.02 | -26.51* | -17.77 | -2.58 | -0.99 | -19.27 | -20.36 | -20.13 | -8.10 | -3.66 | -20.41 | -32.74 | -31.37* | -16.01 |
None | 1.24 | 1.22 | 0.15 | -4.75 | -4.32 | -1.87 | -8.22 | -3.60 | 1.08 | 5.41 | -1.76 | -7.72 | -3.60 | 0.95 | 5.76 |
Nuclear Periphery | -1.12 | -2.71 | -5.45 | -2.11 | 2.17 | 2.95 | -1.96 | -0.11 | -6.18 | 2.19 | 2.84 | -2.43 | -0.30 | -6.40 | 2.60 |
Nuclear Periphery Foci | -2.76 | -2.98 | -5.01 | -4.07 | 2.26 | -0.18 | -1.95 | -1.75 | -1.66 | -1.13 | -0.15 | -2.73 | -2.44 | -2.25 | -0.86 |
Nucleolus | 1.34 | -1.18 | 1.12 | -1.76 | 1.90 | 1.48 | -0.11 | 1.84 | 0.92 | 2.99 | 1.71 | -0.35 | 1.78 | 0.04 | 3.15 |
Nucleus | 1.57 | -1.21 | 1.02 | -1.68 | 1.81 | 0.89 | 0.68 | 0.79 | -0.19 | 0.21 | 1.16 | 0.38 | 0.60 | -0.95 | 0.30 |
Peroxisomes | -0.40 | -1.28 | 3.10 | 5.30 | 4.39 | -1.30 | 5.12 | 2.36 | 3.93 | -3.23 | -2.58 | 3.85 | 2.21 | 5.31 | -2.57 |
Vacuole | -1.72 | -4.69 | -14.80 | -13.34 | -7.67 | 3.05 | -20.02 | -18.24 | -20.79 | -2.05 | 2.68 | -20.52 | -23.79 | -27.18 | -3.25 |
Vacuole Periphery | -0.39 | -2.56 | -6.59 | -8.08 | -2.13 | 8.69 | -12.61 | -10.44 | -17.39 | 0.50 | 8.61 | -12.95 | -9.64 | -19.23 | 3.95 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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