Standard name
Human Ortholog
Description Mitochondrial 15S rRNA-binding protein; required for intron removal of COB and COX1 pre-mRNAs; has separable roles in stabilizing mitochondrial 15S rRNA and in maturation of the COB and COX1 mRNAs; contains pentatricopeptide repeat (PPR) motifs; mutant is respiratory deficient and has defective plasma membrane electron transport

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0.05 0.05 0 0 0 0 0.05 0.06
Bud 0 0 0 0.05 0 0 0.06 0 0.07 0.05 0.06 0.05 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0.13 0 0 0 0 0 0
Cytoplasm 0 0 0.06 0 0.06 0.06 0.05 0 0 0 0 0 0 0.06 0.06 0.09 0 0 0 0 0 0
Endoplasmic Reticulum 0 0.09 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0
Endosome 0.05 0 0.05 0 0.11 0.06 0.05 0 0 0 0 0.09 0.11 0.05 0.09 0.1 0.06 0 0.06 0.06 0.07 0.09
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.36 0.3 0 0.05 0 0 0 0.06
Mitochondria 0.9 0.97 0.71 0.9 0.77 0.82 0.84 0.96 0.94 0.94 0.88 0.86 0.82 0.84 0.19 0.14 0.8 0.81 0.81 0.69 0.59 0.58
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0.05 0.05 0.05 0 0 0 0 0 0 0.1 0.1 0 0 0 0.05 0 0 0.11 0.15 0.12
SpindlePole 0 0 0.08 0 0.05 0.08 0.09 0.06 0.06 0.07 0.11 0 0 0 0.08 0.07 0 0 0 0 0 0
Vac/Vac Membrane 0.09 0.08 0.14 0.06 0.06 0.08 0.08 0.05 0 0 0 0.11 0.08 0.05 0.22 0.16 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 1 4 14 7 6 0 0 1 0 1 3 2 2 1 10 6 3 2 2 4 16 25
Bud 3 4 11 9 13 9 29 15 26 18 24 17 7 9 5 1 0 0 0 1 4 2
Bud Neck 0 0 0 0 1 0 1 1 2 1 0 0 0 0 1 0 0 0 0 2 5 8
Bud Site 1 1 3 1 0 1 7 10 5 9 12 1 0 0 0 0
Cell Periphery 7 3 13 7 8 2 5 2 3 3 11 11 9 9 8 15 0 0 0 0 0 1
Cytoplasm 2 3 15 6 18 15 24 6 9 8 10 9 17 28 11 10 0 0 2 0 1 0
Endoplasmic Reticulum 9 14 9 3 5 4 6 3 4 4 2 3 7 22 4 5 0 0 2 0 3 3
Endosome 20 0 12 4 33 17 24 2 0 3 4 35 42 21 16 12 22 6 17 10 25 36
Golgi 2 2 9 7 11 0 2 3 0 0 4 1 16 10 67 34 14 7 4 5 12 22
Mitochondria 336 152 187 160 225 222 422 349 373 372 341 322 312 372 35 16 301 131 245 116 214 234
Nucleus 2 0 3 1 6 4 13 5 7 8 7 2 0 1 5 1 0 0 0 0 0 1
Nuclear Periphery 11 2 1 1 3 6 7 11 12 8 3 2 1 2 5 5 3 1 1 2 5 2
Nucleolus 10 0 3 1 0 7 7 3 4 5 4 3 1 0 1 1 1 0 0 1 0 1
Peroxisomes 12 0 13 9 14 7 17 2 1 0 4 38 39 18 8 3 17 5 13 18 53 47
SpindlePole 5 1 22 5 15 21 45 20 22 27 41 16 8 6 15 8 1 0 1 1 3 2
Vac/Vac Membrane 34 12 37 10 18 21 39 17 17 17 17 40 30 24 41 18 4 5 6 3 11 10
Unique Cell Count 372 156 262 177 294 272 501 362 397 396 386 374 381 445 187 115 375 162 301 170 363 405
Labelled Cell Count 455 198 352 231 376 336 648 450 485 484 487 502 491 523 232 135 375 162 301 170 363 405


Mitochondria

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 6.0 4.7 5.9 5.5 6.2 4.8 5.0 4.2 4.0 3.8 4.4 5.4 5.5 5.4 7.5 7.3 5.3 5.1 6.0
Std Deviation (1e-4) 0.8 0.7 1.1 1.1 1.6 1.3 1.6 0.9 0.9 0.8 1.0 0.9 1.2 1.1 1.4 1.3 1.3 1.4 1.7
Intensity Change (Log2) -0.12 0.05 -0.31 -0.23 -0.5 -0.56 -0.63 -0.42 -0.14 -0.12 -0.14 0.34 0.3 -0.17 -0.22 0.02


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin
Bud 0 0
Bud Neck 0 0
Bud Site 0 0
Cell Periphery 0 0
Cyto
Endoplasmic Reticulum 0 0
Endosome 0 0
Golgi 0 0
Mitochondria 0 0
Nuclear Periphery 0 0
Nuc
Nucleolus 0 0
Peroxisomes 0 0
SpindlePole 0 0
Vac
Cortical Patches 0 0
Cytoplasm 0 0
Nucleus 0 0
Vacuole 2.2 0.4

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 3.6149 3.7697 3.4996 3.1261 3.055 3.513 1.5121 1.5882 1.6413 1.3537 1.2553 1.5639 1.7423 1.9617 1.59 1.1125 1.0042 1.4074
Actin 0.0232 0.0016 0.0121 0.0048 0.0057 0.0007 0.0378 0.0028 0.0198 0.0339 0.0292 0.0145 0.0582 0.0388 0.0389 0.0025 0.0619 0.0241
Bud 0.0006 0.0004 0.0004 0.0003 0.0022 0.0002 0.0011 0.0003 0.0005 0.0005 0.0004 0.0002 0.0046 0.0026 0.0064 0.0009 0.0006 0.0005
Bud Neck 0.0023 0.0002 0.0026 0.0001 0.0004 0.0023 0.0007 0.0002 0.0005 0.0001 0.0004 0.0005 0.001 0.001 0.0018 0.0059 0.0003 0.0011
Bud Periphery 0.002 0.0007 0.0013 0.0008 0.0033 0.0006 0.0027 0.0006 0.0012 0.0014 0.0007 0.0006 0.0035 0.0035 0.004 0.0031 0.001 0.001
Bud Site 0.0034 0.0024 0.008 0.0002 0.0092 0.0005 0.0045 0.0007 0.0022 0.0005 0.001 0.0004 0.0039 0.0177 0.0141 0.0006 0.0011 0.0019
Cell Periphery 0.0022 0.0003 0.0013 0.0001 0.0003 0.0004 0.0015 0.0001 0.0003 0.0001 0.0001 0.0002 0.0014 0.0005 0.0031 0.0009 0.0001 0.0003
Cytoplasm 0.0028 0.0026 0.0015 0.0001 0.0054 0.0009 0.0012 0.0002 0.0027 0.0001 0.0003 0.0003 0.0225 0.0341 0.023 0.0203 0.0315 0.0322
Cytoplasmic Foci 0.0287 0.0156 0.0166 0.0029 0.018 0.0032 0.0363 0.0128 0.0256 0.0057 0.01 0.0124 0.0378 0.0284 0.0433 0.057 0.0261 0.0287
Eisosomes 0.0031 0.0002 0.001 0.0002 0.0002 0.0002 0.0009 0.0001 0.0019 0.0006 0.0004 0.0005 0.0013 0.0006 0.0013 0.0012 0.0003 0.0003
Endoplasmic Reticulum 0.004 0.0004 0.0009 0 0.0011 0.0002 0.0032 0.0003 0.0008 0.0001 0.0012 0.0001 0.0079 0.0012 0.0008 0.0005 0.0033 0.0005
Endosome 0.0378 0.0267 0.0227 0.0027 0.0294 0.0125 0.0573 0.0205 0.0326 0.0081 0.0319 0.0154 0.0688 0.0559 0.0279 0.0435 0.0608 0.0309
Golgi 0.0412 0.0238 0.0344 0.0083 0.0218 0.012 0.0604 0.0283 0.0301 0.0142 0.0422 0.0282 0.0471 0.0677 0.0413 0.025 0.0528 0.0389
Lipid Particles 0.0668 0.007 0.0224 0.0008 0.0068 0.018 0.0691 0.0104 0.0142 0.0032 0.0217 0.0097 0.0438 0.0168 0.0064 0.0068 0.0245 0.0122
Mitochondria 0.6529 0.8371 0.8099 0.9433 0.8175 0.9033 0.6496 0.8483 0.8163 0.9143 0.8342 0.8651 0.5506 0.6827 0.6566 0.8173 0.6472 0.782
None 0.0268 0.006 0.0187 0.0001 0.0095 0.0127 0.0021 0.0001 0.0018 0.0001 0.0006 0.0001 0.0313 0.0015 0.0767 0.0026 0.0242 0.0189
Nuclear Periphery 0.0035 0.0002 0.0011 0 0.0029 0.0002 0.0097 0.0002 0.0014 0 0.0048 0.0001 0.0283 0.0012 0.0005 0.0002 0.0027 0.0005
Nucleolus 0.0016 0.0007 0.0007 0 0.0014 0.0003 0.0052 0.0001 0.0003 0.0001 0.0002 0.0001 0.0054 0.0015 0.0021 0.0002 0.0002 0.0018
Nucleus 0.0016 0.0005 0.0004 0 0.0021 0.0002 0.0019 0.0001 0.0004 0.0001 0.0005 0.0001 0.0178 0.0008 0.019 0.0003 0.001 0.0014
Peroxisomes 0.0875 0.0642 0.0369 0.0348 0.0374 0.0255 0.0366 0.0692 0.0393 0.0152 0.0159 0.0496 0.0444 0.0372 0.0288 0.0057 0.0411 0.017
Punctate Nuclear 0.0022 0.0002 0.0032 0 0.0147 0.0002 0.0023 0.0003 0.0013 0 0.0011 0.0001 0.0052 0.0016 0.0012 0.0005 0.0012 0.0004
Vacuole 0.0032 0.0068 0.0022 0.0002 0.008 0.0023 0.0103 0.0022 0.003 0.0006 0.0011 0.0007 0.0085 0.0027 0.0016 0.0024 0.0145 0.0028
Vacuole Periphery 0.0025 0.0026 0.0018 0.0004 0.0027 0.0037 0.0056 0.0021 0.0039 0.0011 0.002 0.0012 0.0064 0.0021 0.0011 0.0026 0.0034 0.0028

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 12.5435 6.0817 9.8719 13.9242 9.0964 7.2245 12.5867 13.8776 12.9985 16.5024
Translational Efficiency 0.3422 0.6184 0.4907 0.3546 0.3884 0.6192 0.3817 0.3585 0.4571 0.3398

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1049 1166 212 579 1371 1663 1669 134 2420 2829 1881 713

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 658.11 837.14 1054.35 959.17 798.98 803.03 917.88 962.93 737.92 817.09 933.26 959.88
Standard Deviation 79.41 107.47 140.00 113.31 95.27 121.19 114.69 170.19 112.91 116.94 125.47 125.98
Intensity Change Log 2 0.347140 0.679953 0.543458 0.007294 0.200146 0.269272 0.170755 0.436738 0.399593

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000354 0.000642 0.011144 0.018015 0.001020 0.002004 0.003722 0.010791 0.000732 0.001443 0.004559 0.016657
Bud Neck 0.014335 0.002693 0.015077 0.004277 0.001386 0.003225 0.009729 0.003890 0.006999 0.003006 0.010331 0.004205
Bud Site 0.012955 0.006135 0.070010 0.054336 0.003126 0.005821 0.019251 0.016585 0.007387 0.005950 0.024971 0.047241
Cell Periphery 0.000484 0.000161 0.000416 0.001073 0.000296 0.000259 0.000316 0.000578 0.000378 0.000219 0.000327 0.000980
Cytoplasm 0.011709 0.002261 0.003622 0.009607 0.001113 0.003222 0.007924 0.005331 0.005706 0.002826 0.007439 0.008804
Cytoplasmic Foci 0.055615 0.043635 0.089563 0.078206 0.030088 0.042118 0.072372 0.084379 0.041153 0.042743 0.074310 0.079366
Eisosomes 0.000104 0.000050 0.000121 0.000147 0.000054 0.000073 0.000102 0.000148 0.000076 0.000064 0.000104 0.000147
Endoplasmic Reticulum 0.000894 0.000285 0.003876 0.001137 0.000348 0.000368 0.001474 0.001344 0.000585 0.000334 0.001745 0.001176
Endosome 0.073118 0.095303 0.121189 0.097007 0.078143 0.089620 0.113813 0.132089 0.075965 0.091962 0.114644 0.103600
Golgi 0.075217 0.094438 0.100673 0.155913* 0.088762 0.109581 0.094154 0.167863* 0.082890 0.103340 0.094889 0.158159*
Lipid Particles 0.009889 0.001462 0.002261 0.000661 0.004134 0.004778 0.010127 0.011772 0.006629 0.003411 0.009240 0.002750
Mitochondria 0.631800* 0.665154* 0.212422* 0.410850* 0.728358* 0.669840* 0.454067* 0.413936* 0.686503* 0.667909* 0.426832* 0.411430*
Mitotic Spindle 0.006146 0.021161 0.160422* 0.054302 0.007410 0.005649 0.072264 0.017302 0.006862 0.012042 0.082200 0.047348
None 0.002443 0.000397 0.000275 0.000196 0.000153 0.000405 0.001385 0.000500 0.001145 0.000402 0.001260 0.000253
Nuclear Periphery 0.000348 0.000146 0.001910 0.000131 0.000207 0.000121 0.001545 0.000807 0.000268 0.000131 0.001586 0.000258
Nuclear Periphery Foci 0.000274 0.000258 0.002603 0.000350 0.000240 0.000211 0.001366 0.000596 0.000255 0.000231 0.001506 0.000396
Nucleolus 0.003628 0.000665 0.002851 0.000080 0.000236 0.000665 0.002854 0.000265 0.001707 0.000665 0.002853 0.000115
Nucleus 0.005029 0.001308 0.008342 0.000351 0.000240 0.001759 0.005935 0.000544 0.002316 0.001573 0.006206 0.000387
Peroxisomes 0.050716 0.034181 0.076313 0.078769 0.029208 0.040554 0.063450 0.053024 0.038531 0.037927 0.064900 0.073931
Vacuole 0.018388 0.015021 0.074382 0.021565 0.009814 0.009319 0.043274 0.035029 0.013531 0.011669 0.046780 0.024095
Vacuole Periphery 0.026552 0.014643 0.042528 0.013028 0.015662 0.010407 0.020876 0.043228 0.020383 0.012153 0.023316 0.018704

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -2.17 -5.25 -5.59 -5.50 -1.63 -1.63 -4.44 -2.39 -2.13 -1.72 -1.99 -6.91 -5.94 -5.64 -4.40
Bud Neck 6.14 -0.31 4.98 -1.70 4.08 -2.72 -7.12 -2.13 -0.51 3.65 4.34 -2.59 2.56 -1.49 4.87
Bud Site 4.09 -6.47 -7.66 -9.15 1.61 -2.56 -10.07 -4.20 -3.32 0.82 1.49 -9.69 -9.13 -9.50 -4.76
Cell Periphery 6.28 1.04 -1.40 -2.18 -1.57 0.37 -0.19 -2.04 -3.17 -2.51 2.61 0.75 -1.75 -2.25 -1.92
Cytoplasm 5.20 4.50 0.81 -6.63 -5.58 -2.90 -6.54 -1.95 -0.97 0.98 3.08 -1.62 -2.79 -5.81 -1.15
Cytoplasmic Foci 3.21 -4.05 -3.98 -6.93 1.29 -4.90 -13.81 -5.92 -4.58 -1.09 -0.58 -11.13 -8.84 -8.71 -0.73
Eisosomes 8.94 -1.80 -4.00 -10.86 -1.88 -4.54 -7.49 -3.68 -2.92 -1.72 3.37 -4.79 -7.87 -9.52 -4.13
Endoplasmic Reticulum 3.20 -4.48 -0.93 -4.07 4.07 -0.17 -6.08 -2.67 -2.58 0.37 2.24 -6.18 -2.97 -4.44 2.39
Endosome -3.95 -4.81 -3.60 -0.20 2.23 -2.29 -6.66 -3.85 -3.05 -1.33 -4.29 -8.69 -4.78 -2.00 1.76
Golgi -3.30 -2.40 -8.68 -6.58 -4.47 -3.55 -0.84 -4.38 -3.23 -4.15 -4.88 -2.51 -9.30 -6.75 -7.75
Lipid Particles 5.60 4.80 6.16 2.56 3.05 -0.52 -3.84 -1.46 -1.34 -0.27 3.36 -1.93 3.01 0.58 4.32
Mitochondria -2.41 19.81 11.89 13.99 -8.25 4.93 22.62 9.94 8.10 1.16 1.98 24.61 18.03 17.00 0.83
Mitotic Spindle -7.07 -10.07 -8.43 -5.65 6.41 1.28 -15.67 -2.74 -3.36 10.41 -4.30 -18.66 -8.57 -7.48 5.40
None 3.70 4.17 4.32 1.08 2.61 -2.15 -2.70 -1.32 -0.42 1.68 2.96 -0.51 3.79 1.22 2.48
Nuclear Periphery 2.32 -2.43 2.65 0.25 2.78 0.65 -3.41 -0.94 -1.09 1.04 1.65 -3.80 0.05 -1.05 3.70
Nuclear Periphery Foci 0.18 -4.30 -0.75 -0.78 4.08 0.29 -6.02 -1.87 -2.12 3.27 0.36 -7.35 -1.45 -1.73 6.01
Nucleolus 2.90 0.49 3.60 1.84 1.90 -1.14 -4.81 -0.32 1.03 4.73 2.14 -2.08 3.68 2.15 5.34
Nucleus 3.02 -0.87 4.18 1.71 2.23 -2.60 -5.18 -1.33 1.93 4.81 1.09 -3.45 3.83 2.76 5.47
Peroxisomes 3.38 -2.52 -3.35 -5.58 -0.09 -2.81 -7.77 -2.06 -1.07 0.93 0.23 -6.62 -5.25 -5.37 -1.14
Vacuole 1.68 -7.31 -1.45 -2.60 6.50 0.39 -13.76 -3.35 -3.41 1.09 1.69 -14.18 -4.30 -5.01 6.52
Vacuole Periphery 4.94 -2.87 5.11 0.89 5.56 3.93 -3.13 -2.87 -3.43 -2.34 6.37 -1.69 0.68 -2.82 1.78
Standard name
Human Ortholog
Description Mitochondrial 15S rRNA-binding protein; required for intron removal of COB and COX1 pre-mRNAs; has separable roles in stabilizing mitochondrial 15S rRNA and in maturation of the COB and COX1 mRNAs; contains pentatricopeptide repeat (PPR) motifs; mutant is respiratory deficient and has defective plasma membrane electron transport
Localization
Cell Percentages mitochondrion (96%)
Cell Cycle Regulation No
Subcompartmental Group mito-2

Ccm1

Ccm1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)

Molecular Function (Gene Ontology)

Cellular Component (Gene Ontology)


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Ccm1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available