Standard name | |
---|---|
Human Ortholog | |
Description | Cystathionine beta-synthase; catalyzes synthesis of cystathionine from serine and homocysteine, the first committed step in cysteine biosynthesis; responsible for hydrogen sulfide generation; advances passage through START by promoting cell growth which requires catalytic activity, and reducing critical cell size independent of catalytic activity; mutations in human ortholog CBS cause homocystinuria; human CBS can complement yeast null mutant |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 4 | 4 | 12 | 4 | 11 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 1 | 1 | 1 | 2 | 2 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 5 | 2 | 0 | 2 | 5 | 7 | 9 | 6 | 20 | 9 | 13 | 4 | 3 | 4 | 9 | 5 | 3 | 0 | 0 | 0 | 0 | 1 | 0 |
Cytoplasm | 215 | 265 | 185 | 84 | 149 | 135 | 224 | 162 | 124 | 197 | 110 | 130 | 177 | 225 | 292 | 415 | 225 | 171 | 198 | 260 | 176 | 94 | 212 | 181 |
Endoplasmic Reticulum | 1 | 5 | 2 | 0 | 3 | 2 | 12 | 17 | 14 | 14 | 21 | 13 | 2 | 2 | 6 | 18 | 14 | 8 | 1 | 0 | 5 | 3 | 5 | 10 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 2 | 2 | 0 | 0 | 0 | 0 | 4 | 1 | 1 | 1 | 0 | 0 | 0 | 1 | 0 |
Mitochondria | 2 | 2 | 0 | 0 | 0 | 1 | 0 | 1 | 3 | 5 | 3 | 11 | 0 | 0 | 0 | 3 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 2 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vac/Vac Membrane | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 1 | 1 | 1 | 0 | 0 | 1 | 1 | 2 | 0 | 0 | 3 | 1 | 0 | 2 | 3 |
Unique Cell Count | 218 | 270 | 187 | 84 | 150 | 139 | 239 | 186 | 140 | 229 | 144 | 164 | 180 | 227 | 298 | 426 | 233 | 173 | 208 | 268 | 189 | 103 | 232 | 206 |
Labelled Cell Count | 219 | 277 | 189 | 84 | 154 | 144 | 246 | 196 | 153 | 252 | 151 | 180 | 183 | 230 | 303 | 453 | 248 | 183 | 208 | 268 | 189 | 103 | 232 | 206 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 54.0 | 62.2 | 51.2 | 55.7 | 54.2 | 49.2 | 43.9 | 43.5 | 39.0 | 37.0 | 37.4 | 33.6 | 73.8 | 72.1 | 69.9 | 69.8 | 79.6 | 93.2 | 57.8 | 61.1 | 58.6 |
Std Deviation (1e-4) | 12.7 | 13.9 | 10.7 | 12.9 | 12.9 | 10.9 | 10.8 | 9.8 | 9.5 | 8.3 | 9.7 | 8.6 | 18.8 | 19.1 | 20.3 | 25.9 | 26.7 | 31.4 | 19.7 | 17.8 | 17.9 |
Intensity Change (Log2) | – | – | – | 0.12 | 0.08 | -0.06 | -0.22 | -0.24 | -0.39 | -0.47 | -0.45 | -0.61 | 0.53 | 0.49 | 0.45 | 0.45 | 0.64 | 0.86 | 0.18 | 0.25 | 0.19 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 3.2 | 0 | 3.6 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 2.2 | 0 | 3.5 | 0 | 3.2 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 1.0 | 0.4 | -1.2 | -2.7 | -4.5 | -4.1 | -4.8 | -6.5 | -6.1 | -0.5 | 0.2 | -0.8 | -1.2 | -1.6 | -0.1 |
Endoplasmic Reticulum | 0 | 0 | 0 | 2.3 | 3.5 | 3.7 | 2.7 | 4.8 | 3.2 | 0 | 0 | 0 | 2.0 | 2.6 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3.6 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 49.0194 | 68.283 | 61.1799 | 59.4466 | 51.7966 | 62.1804 | 56.1056 | 72.8814 | 65.4398 | 63.2037 | 59.7565 | 69.8963 | 50.7126 | 72.7921 | 64.6481 | 53.7608 | 58.418 | 63.7471 |
Actin | 0.0054 | 0.0002 | 0.0046 | 0.0006 | 0.0002 | 0.0144 | 0.0037 | 0.0001 | 0.0032 | 0.0001 | 0.0009 | 0.0008 | 0.0177 | 0.0001 | 0.0115 | 0.0013 | 0.026 | 0.0003 |
Bud | 0.0012 | 0.0002 | 0.0007 | 0.0007 | 0.0004 | 0.001 | 0.0013 | 0.0001 | 0.0006 | 0.0005 | 0.0008 | 0.0003 | 0.0008 | 0.0005 | 0.0009 | 0.001 | 0.0007 | 0.0002 |
Bud Neck | 0.001 | 0.0003 | 0.0007 | 0.0006 | 0.0004 | 0.0132 | 0.0003 | 0.0002 | 0.0021 | 0.0002 | 0.0004 | 0.0009 | 0.0006 | 0.0002 | 0.0003 | 0.0008 | 0.0003 | 0.0008 |
Bud Periphery | 0.0014 | 0.0001 | 0.0005 | 0.0002 | 0.0005 | 0.001 | 0.0013 | 0 | 0.0005 | 0 | 0.0006 | 0.0001 | 0.0008 | 0.0003 | 0.0006 | 0.0034 | 0.0017 | 0 |
Bud Site | 0.0028 | 0.0002 | 0.0005 | 0.0003 | 0.0002 | 0.0003 | 0.001 | 0.0004 | 0.0045 | 0.0001 | 0.0007 | 0 | 0.0032 | 0.0008 | 0.003 | 0.0002 | 0.0004 | 0 |
Cell Periphery | 0.004 | 0.0024 | 0.0049 | 0.001 | 0.0013 | 0.0006 | 0.0014 | 0.0003 | 0.001 | 0.0004 | 0.0018 | 0.0001 | 0.0014 | 0.0006 | 0.0003 | 0.0016 | 0.0007 | 0 |
Cytoplasm | 0.7575 | 0.9662 | 0.9005 | 0.8508 | 0.8085 | 0.8706 | 0.845 | 0.9897 | 0.9399 | 0.9672 | 0.8184 | 0.9742 | 0.8832 | 0.9877 | 0.9319 | 0.8742 | 0.874 | 0.9596 |
Cytoplasmic Foci | 0.0157 | 0.0004 | 0.0049 | 0.0165 | 0.0217 | 0.0027 | 0.0147 | 0.0005 | 0.0052 | 0.0014 | 0.0154 | 0.0035 | 0.0102 | 0.0004 | 0.0114 | 0.0233 | 0.0076 | 0.0019 |
Eisosomes | 0.0004 | 0 | 0.0001 | 0 | 0.0001 | 0.0001 | 0.0001 | 0 | 0.0001 | 0 | 0.0006 | 0 | 0.0005 | 0 | 0.0003 | 0 | 0.0008 | 0 |
Endoplasmic Reticulum | 0.0399 | 0.0117 | 0.0078 | 0.0188 | 0.0019 | 0.034 | 0.0223 | 0.0033 | 0.0081 | 0.0018 | 0.0054 | 0.0076 | 0.0144 | 0.0032 | 0.0028 | 0.0188 | 0.0193 | 0.0274 |
Endosome | 0.0085 | 0.0006 | 0.0038 | 0.0036 | 0.0072 | 0.0111 | 0.0097 | 0.0007 | 0.0021 | 0.0025 | 0.0056 | 0.0057 | 0.0128 | 0.0003 | 0.0055 | 0.0342 | 0.0082 | 0.003 |
Golgi | 0.0014 | 0.0001 | 0.0009 | 0.0004 | 0.0004 | 0.0009 | 0.0016 | 0.0001 | 0.0005 | 0.0001 | 0.0016 | 0.0005 | 0.0022 | 0 | 0.0018 | 0.0052 | 0.0046 | 0.0004 |
Lipid Particles | 0.0065 | 0.0003 | 0.0019 | 0.0063 | 0.0054 | 0.0001 | 0.0076 | 0 | 0.0022 | 0.0001 | 0.0191 | 0 | 0.0048 | 0.0001 | 0.0034 | 0.0105 | 0.0033 | 0 |
Mitochondria | 0.0109 | 0.0001 | 0.008 | 0.0008 | 0.0094 | 0.0007 | 0.0086 | 0 | 0.0052 | 0.0001 | 0.0185 | 0.0003 | 0.0042 | 0.0001 | 0.0098 | 0.002 | 0.0232 | 0.0005 |
None | 0.0052 | 0.0001 | 0.0006 | 0.0014 | 0.0199 | 0.0002 | 0.0058 | 0.0001 | 0.0019 | 0.0002 | 0.0102 | 0.0002 | 0.0055 | 0.0001 | 0.0038 | 0.0005 | 0.0083 | 0.0003 |
Nuclear Periphery | 0.0392 | 0.0023 | 0.0082 | 0.005 | 0.0075 | 0.0052 | 0.0149 | 0.0007 | 0.0027 | 0.0015 | 0.0066 | 0.0015 | 0.0083 | 0.0007 | 0.0014 | 0.0031 | 0.0042 | 0.0011 |
Nucleolus | 0.0042 | 0 | 0.0012 | 0.0002 | 0.021 | 0 | 0.0019 | 0 | 0.0013 | 0 | 0.0131 | 0 | 0.0022 | 0.0001 | 0.0003 | 0.0001 | 0.0002 | 0 |
Nucleus | 0.0112 | 0.0013 | 0.0047 | 0.0028 | 0.0119 | 0.0019 | 0.0065 | 0.0006 | 0.0023 | 0.0014 | 0.0205 | 0.001 | 0.0051 | 0.0007 | 0.0014 | 0.0024 | 0.0018 | 0.0011 |
Peroxisomes | 0.0019 | 0 | 0.0026 | 0.001 | 0.0004 | 0.0001 | 0.0044 | 0 | 0.0034 | 0 | 0.0012 | 0 | 0.0006 | 0 | 0.0008 | 0.0004 | 0.0002 | 0 |
Punctate Nuclear | 0.0038 | 0 | 0.0003 | 0.001 | 0.0032 | 0 | 0.0016 | 0 | 0.0012 | 0 | 0.0161 | 0.0001 | 0.0036 | 0 | 0.0011 | 0.0003 | 0.0006 | 0 |
Vacuole | 0.0599 | 0.0125 | 0.0344 | 0.0733 | 0.0395 | 0.0323 | 0.0342 | 0.0028 | 0.0095 | 0.0199 | 0.0284 | 0.0023 | 0.0155 | 0.0039 | 0.0056 | 0.0135 | 0.0116 | 0.0028 |
Vacuole Periphery | 0.0182 | 0.001 | 0.0082 | 0.0146 | 0.0389 | 0.0097 | 0.0119 | 0.0002 | 0.0027 | 0.0024 | 0.0141 | 0.0008 | 0.0024 | 0.0002 | 0.0022 | 0.003 | 0.0023 | 0.0006 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 503.917 | 496.4242 | 626.7542 | 595.5675 | 448.709 | 442.1305 | 417.6785 | 426.2251 | 519.4414 | 398.8587 |
Translational Efficiency | 1.4651 | 1.4877 | 1.5137 | 1.7396 | 1.4468 | 1.4631 | 1.5271 | 1.5077 | 1.4479 | 1.5412 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
1851 | 1268 | 264 | 1682 | 1840 | 1312 | 164 | 74 | 3691 | 2580 | 428 | 1756 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 3332.81 | 4027.94 | 8140.60 | 7925.08 | 3603.75 | 4008.11 | 8203.30 | 8935.21 | 3467.88 | 4017.86 | 8164.63 | 7967.65 |
Standard Deviation | 726.82 | 967.71 | 1444.48 | 1817.15 | 726.76 | 904.88 | 1360.18 | 1965.35 | 739.31 | 936.34 | 1413.10 | 1834.90 |
Intensity Change Log 2 | — | 0.273303 | 1.288396 | 1.249686 | — | 0.153423 | 1.186705 | 1.310003 | — | 0.212266 | 1.236460 | 1.281337 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.000037 | 0.000102 | 0.000195 | 0.000157 | 0.000048 | 0.000123 | 0.000129 | 0.000166 | 0.000042 | 0.000112 | 0.000170 | 0.000157 |
Bud Neck | 0.000145 | 0.000747 | 0.008855 | 0.001236 | 0.000205 | 0.000571 | 0.003729 | 0.002905 | 0.000175 | 0.000658 | 0.006891 | 0.001306 |
Bud Site | 0.002820 | 0.003154 | 0.003391 | 0.006076 | 0.002093 | 0.003690 | 0.002209 | 0.005110 | 0.002457 | 0.003426 | 0.002938 | 0.006035 |
Cell Periphery | 0.001784 | 0.009220 | 0.033705 | 0.010883 | 0.004985 | 0.008562 | 0.017646 | 0.128468* | 0.003380 | 0.008885 | 0.027552 | 0.015838 |
Cytoplasm | 0.938805* | 0.834486* | 0.314230* | 0.766493* | 0.885795* | 0.857518* | 0.503402* | 0.402326* | 0.912379* | 0.846198* | 0.386716* | 0.751146* |
Cytoplasmic Foci | 0.006510 | 0.009589 | 0.025727 | 0.004091 | 0.006576 | 0.009326 | 0.020477 | 0.009263 | 0.006543 | 0.009455 | 0.023715 | 0.004309 |
Eisosomes | 0.000000 | 0.000002 | 0.000012 | 0.000002 | 0.000001 | 0.000002 | 0.000005 | 0.000008 | 0.000001 | 0.000002 | 0.000009 | 0.000003 |
Endoplasmic Reticulum | 0.000454 | 0.000414 | 0.000227 | 0.000484 | 0.000558 | 0.000459 | 0.000090 | 0.000089 | 0.000506 | 0.000437 | 0.000175 | 0.000467 |
Endosome | 0.000416 | 0.000655 | 0.000917 | 0.000604 | 0.000503 | 0.000714 | 0.000538 | 0.008875 | 0.000459 | 0.000685 | 0.000771 | 0.000953 |
Golgi | 0.016219 | 0.040346 | 0.035961 | 0.007331 | 0.038696 | 0.038477 | 0.031676 | 0.012995 | 0.027424 | 0.039396 | 0.034319 | 0.007570 |
Lipid Particles | 0.002218 | 0.010467 | 0.028157 | 0.008887 | 0.006303 | 0.008405 | 0.013566 | 0.036961 | 0.004255 | 0.009419 | 0.022566 | 0.010070 |
Mitochondria | 0.000100 | 0.000103 | 0.000191 | 0.000186 | 0.000068 | 0.000111 | 0.000124 | 0.000109 | 0.000084 | 0.000107 | 0.000165 | 0.000182 |
Mitotic Spindle | 0.000749 | 0.001878 | 0.002389 | 0.000617 | 0.000833 | 0.001554 | 0.001078 | 0.000783 | 0.000791 | 0.001713 | 0.001887 | 0.000624 |
None | 0.002571 | 0.002753 | 0.001460 | 0.001728 | 0.001670 | 0.002259 | 0.001238 | 0.000793 | 0.002122 | 0.002502 | 0.001375 | 0.001689 |
Nuclear Periphery | 0.000050 | 0.000472 | 0.004261 | 0.000185 | 0.000200 | 0.000329 | 0.002668 | 0.000888 | 0.000124 | 0.000399 | 0.003650 | 0.000215 |
Nuclear Periphery Foci | 0.002784 | 0.014003 | 0.016366 | 0.002875 | 0.008047 | 0.010239 | 0.014395 | 0.005664 | 0.005408 | 0.012089 | 0.015611 | 0.002993 |
Nucleolus | 0.000020 | 0.000183 | 0.000201 | 0.000187 | 0.000024 | 0.000080 | 0.000172 | 0.000136 | 0.000022 | 0.000130 | 0.000190 | 0.000184 |
Nucleus | 0.004427 | 0.014956 | 0.040859 | 0.016552 | 0.004736 | 0.011650 | 0.055262 | 0.021495 | 0.004581 | 0.013275 | 0.046378 | 0.016761 |
Peroxisomes | 0.000014 | 0.000018 | 0.000017 | 0.000048 | 0.000012 | 0.000014 | 0.000012 | 0.000390 | 0.000013 | 0.000016 | 0.000016 | 0.000062 |
Vacuole | 0.008414 | 0.039275 | 0.421750* | 0.158663 | 0.018314 | 0.031219 | 0.292643* | 0.339371* | 0.013349 | 0.035178 | 0.372279* | 0.166279 |
Vacuole Periphery | 0.011462 | 0.017180 | 0.061129 | 0.012715 | 0.020335 | 0.014698 | 0.038943 | 0.023203 | 0.015886 | 0.015918 | 0.052628 | 0.013157 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -6.24 | -8.63 | -4.18 | -2.11 | 1.76 | -3.69 | -6.27 | -4.19 | -1.54 | -0.77 | -6.03 | -10.30 | -4.22 | -1.82 | 1.04 |
Bud Neck | -7.58 | -8.16 | -9.93 | -5.46 | 7.03 | -6.16 | -7.22 | -4.75 | -4.34 | 0.42 | -9.77 | -9.69 | -10.65 | -7.21 | 7.81 |
Bud Site | -0.86 | -2.78 | -4.93 | -4.58 | -2.77 | -2.76 | -0.75 | -2.92 | -1.72 | -2.55 | -2.77 | -2.91 | -5.68 | -4.25 | -3.89 |
Cell Periphery | -8.79 | -9.88 | -11.35 | -0.62 | 6.95 | -4.15 | -4.36 | -5.20 | -5.00 | -4.58 | -9.11 | -10.43 | -9.27 | -3.89 | 4.87 |
Cytoplasm | 13.63 | 32.68 | 25.60 | 8.86 | -21.55 | 3.85 | 15.88 | 12.70 | 11.80 | 1.51 | 12.28 | 33.72 | 24.18 | 13.50 | -20.71 |
Cytoplasmic Foci | -3.78 | -6.68 | 2.16 | 5.57 | 7.07 | -3.89 | -6.12 | -1.97 | -1.05 | 2.90 | -5.40 | -8.70 | 2.08 | 6.32 | 9.07 |
Eisosomes | -4.67 | -8.76 | -6.72 | -2.31 | 7.24 | -4.83 | -6.67 | -6.22 | -5.47 | -1.80 | -6.66 | -10.17 | -7.39 | -3.60 | 7.59 |
Endoplasmic Reticulum | 0.43 | 2.79 | 0.14 | -0.02 | -0.81 | 0.93 | 7.72 | 7.76 | 4.58 | 0.55 | 0.95 | 6.34 | 0.41 | 0.14 | -0.98 |
Endosome | -3.46 | -5.55 | -1.34 | 0.43 | 2.84 | -2.12 | -1.53 | -0.98 | -0.95 | -0.96 | -3.69 | -5.51 | -1.35 | -0.55 | 0.21 |
Golgi | -8.69 | -6.30 | 6.47 | 13.43 | 11.05 | 0.06 | 3.09 | 9.34 | 8.96 | 4.94 | -5.84 | -1.70 | 15.21 | 18.85 | 13.06 |
Lipid Particles | -9.29 | -10.97 | -8.80 | 1.58 | 7.91 | -2.57 | -5.28 | -4.24 | -3.85 | -2.88 | -8.29 | -11.56 | -7.05 | -0.28 | 7.52 |
Mitochondria | -0.31 | -3.94 | -2.49 | -1.98 | 0.82 | -1.74 | -3.98 | -0.48 | 0.36 | 0.91 | -1.45 | -5.11 | -2.92 | -2.02 | 0.22 |
Mitotic Spindle | -1.55 | -2.04 | -0.27 | 1.43 | 1.95 | -1.55 | -1.35 | -0.07 | 1.29 | 1.26 | -2.14 | -2.42 | -0.16 | 1.93 | 2.31 |
None | -0.37 | 2.49 | 1.84 | 3.62 | -0.76 | -2.84 | 2.16 | 4.23 | 7.65 | 2.60 | -1.46 | 3.00 | 1.55 | 3.35 | -0.93 |
Nuclear Periphery | -8.62 | -9.16 | -11.62 | 5.12 | 8.83 | -3.13 | -4.85 | -5.39 | -4.26 | 3.47 | -8.63 | -10.21 | -6.34 | 4.79 | 9.89 |
Nuclear Periphery Foci | -9.85 | -9.11 | -0.70 | 9.70 | 8.99 | -2.49 | -3.66 | 2.51 | 4.10 | 4.70 | -9.22 | -8.94 | 6.60 | 12.69 | 11.03 |
Nucleolus | -1.35 | -13.15 | -9.34 | 0.05 | 1.41 | -2.67 | -7.99 | -3.11 | -1.79 | 0.40 | -1.76 | -15.15 | -9.53 | -0.78 | 1.00 |
Nucleus | -10.07 | -8.21 | -15.45 | -4.23 | 5.09 | -6.72 | -7.63 | -5.00 | -3.34 | 4.29 | -11.85 | -11.18 | -16.15 | -6.55 | 7.33 |
Peroxisomes | -0.74 | -3.37 | -7.08 | -4.29 | -4.97 | -1.17 | -1.65 | -1.24 | -1.22 | -1.22 | -1.19 | -3.80 | -3.98 | -3.60 | -3.31 |
Vacuole | -10.77 | -22.95 | -28.69 | -20.93 | 12.96 | -5.41 | -12.97 | -10.82 | -10.35 | -1.37 | -11.69 | -25.56 | -29.36 | -24.38 | 12.55 |
Vacuole Periphery | -3.44 | -8.62 | -0.37 | 3.52 | 8.62 | 2.94 | -2.49 | 0.40 | -0.64 | 2.01 | -0.04 | -8.24 | 3.26 | 3.17 | 9.39 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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