Standard name | |
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Human Ortholog | |
Description | Component of transport protein particle (TRAPP) complex II; TRAPPII is a multimeric guanine nucleotide-exchange factor for the GTPase Ypt1p, regulating intra-Golgi and endosome-Golgi traffic; role in cell wall beta-glucan biosynthesis and the stress response |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 1 | 0 | 14 | 37 | 13 | 8 | 12 | – | 6 | 4 | 4 | 10 | 0 | 3 | 0 | 10 | 12 | 15 | 6 | 1 | 5 | 0 | 1 | 3 |
Bud | 11 | 2 | 1 | 8 | 9 | 15 | 37 | – | 18 | 29 | 35 | 48 | 0 | 4 | 2 | 2 | 9 | 7 | 1 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 2 | 3 | 2 | 4 | 2 | – | 4 | 1 | 2 | 3 | 0 | 1 | 0 | 2 | 1 | 0 | 1 | 0 | 1 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 1 | 1 | 4 | 8 | – | 9 | 6 | 20 | 14 | 0 | 0 | 0 | 0 | 0 | 1 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 1 | 0 | 1 | 1 | 1 | – | 0 | 2 | 1 | 1 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 11 | 0 | 10 | 31 | 19 | 14 | 20 | – | 18 | 19 | 14 | 21 | 3 | 7 | 20 | 16 | 24 | 14 | 1 | 0 | 0 | 0 | 1 | 2 |
Endoplasmic Reticulum | 6 | 0 | 9 | 9 | 9 | 6 | 4 | – | 2 | 2 | 4 | 4 | 0 | 1 | 0 | 5 | 10 | 6 | 0 | 0 | 0 | 0 | 0 | 1 |
Endosome | 35 | 0 | 16 | 24 | 61 | 49 | 70 | – | 25 | 30 | 15 | 53 | 5 | 26 | 37 | 62 | 59 | 60 | 105 | 55 | 56 | 4 | 12 | 20 |
Golgi | 5 | 2 | 6 | 10 | 4 | 2 | 2 | – | 0 | 0 | 0 | 2 | 2 | 4 | 0 | 14 | 27 | 48 | 46 | 12 | 18 | 0 | 5 | 17 |
Mitochondria | 167 | 21 | 8 | 37 | 63 | 165 | 227 | – | 225 | 171 | 367 | 298 | 2 | 3 | 9 | 22 | 29 | 19 | 28 | 12 | 54 | 2 | 16 | 30 |
Nucleus | 0 | 0 | 2 | 0 | 3 | 0 | 8 | – | 9 | 5 | 2 | 4 | 1 | 0 | 2 | 0 | 0 | 2 | 0 | 1 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 1 | 0 | 0 | 0 | 0 | 1 | 0 | – | 0 | 3 | 2 | 5 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 |
Nucleolus | 2 | 0 | 0 | 0 | 0 | 12 | 6 | – | 13 | 16 | 16 | 23 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 8 | 0 | 2 | 1 | 23 | 24 | 41 | – | 17 | 31 | 14 | 49 | 7 | 3 | 5 | 24 | 2 | 4 | 49 | 6 | 40 | 2 | 5 | 9 |
SpindlePole | 22 | 0 | 39 | 47 | 72 | 69 | 110 | – | 77 | 104 | 56 | 152 | 4 | 19 | 22 | 55 | 35 | 55 | 10 | 5 | 3 | 1 | 1 | 1 |
Vac/Vac Membrane | 10 | 2 | 4 | 9 | 15 | 7 | 5 | – | 5 | 7 | 1 | 5 | 0 | 3 | 3 | 10 | 17 | 20 | 4 | 3 | 4 | 0 | 2 | 3 |
Unique Cell Count | 226 | 21 | 92 | 165 | 230 | 294 | 398 | 330 | 314 | 425 | 494 | 19 | 60 | 92 | 155 | 163 | 184 | 261 | 104 | 190 | 16 | 47 | 98 | |
Labelled Cell Count | 279 | 27 | 114 | 217 | 295 | 381 | 553 | 428 | 430 | 553 | 692 | 24 | 74 | 101 | 223 | 226 | 251 | 261 | 104 | 190 | 16 | 47 | 98 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 5.0 | 5.4 | 6.1 | 6.0 | 6.3 | 5.4 | 5.4 | – | 4.7 | 4.9 | 4.3 | 4.8 | 6.7 | 6.7 | 6.2 | 7.7 | 7.7 | 7.4 | – | – | 6.2 |
Std Deviation (1e-4) | 0.7 | 0.5 | 0.6 | 0.8 | 1.1 | 0.9 | 1.2 | – | 0.9 | 0.8 | 0.8 | 1.1 | 1.6 | 1.5 | 1.3 | 1.3 | 1.3 | 1.2 | – | – | 1.1 |
Intensity Change (Log2) | – | – | – | -0.03 | 0.04 | -0.2 | -0.18 | – | -0.4 | -0.33 | -0.52 | -0.35 | 0.12 | 0.13 | 0.02 | 0.34 | 0.34 | 0.27 | – | – | 0.01 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud | 0 | 0 | 0 | 2.7 | – | 0 | 2.6 | – | – | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | – | 0 | 0 | – | – | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | – | 0 | 0 | – | – | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | – | 0 | 0 | – | – | 0 | 0 | 0 | 0 | 0 | 0 |
Cyto | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endoplasmic Reticulum | -1.3 | -2.1 | 0 | 0 | – | 0 | 0 | – | – | 0 | 0 | 0 | -2.2 | -1.1 | -2.3 |
Endosome | -0.6 | 1.7 | -0.2 | 0 | – | 0 | -2.1 | – | – | 0 | 3.5 | 3.4 | 3.7 | 3.2 | 2.7 |
Golgi | -0.1 | 0 | 0 | 0 | – | 0 | 0 | – | – | 0 | 0 | 0 | 0.7 | 2.3 | 3.9 |
Mitochondria | 2.8 | 3.7 | 8.0 | 8.4 | – | 0 | 7.8 | – | – | 0 | 0 | 0.3 | 1.3 | 2.0 | 0.4 |
Nuclear Periphery | 0 | 0 | 0 | 0 | – | 0 | 0 | – | – | 0 | 0 | 0 | 0 | 0 | 0 |
Nuc | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleolus | 0 | 0 | 0 | 0 | – | 0 | 0 | – | – | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 2.4 | 2.0 | 2.5 | – | 0 | 2.4 | – | – | 0 | 0 | 0 | 3.3 | 0 | 0 |
SpindlePole | -2.3 | -1.9 | -3.5 | -2.8 | – | 0 | -1.6 | – | – | -1.7 | -1.3 | -2.7 | -1.1 | -3.5 | -2.1 |
Vac | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cortical Patches | 1.4 | -2.8 | -4.5 | 0 | – | 0 | 0 | – | – | 0 | -2.0 | -3.9 | -2.2 | -2.0 | -1.8 |
Cytoplasm | 1.7 | -0.7 | -2.1 | -2.1 | – | -1.8 | -1.6 | – | – | 0 | 0.2 | 2.0 | -0.1 | 0.9 | -0.9 |
Nucleus | 0 | 0 | 0 | 0 | – | 0 | 0 | – | – | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 0.7 | 0 | 0 | – | 0 | 0 | – | – | 0 | 0 | 0 | 0.7 | 1.7 | 1.8 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 1.0572 | 1.5895 | 1.4874 | 1.4712 | 0.8764 | 1.6106 | 0.9305 | 1.667 | 1.3868 | 0.9429 | 1.3214 | 1.2728 | 0.2581 | 0.6276 | 0.2831 | 0.3473 | 0.5757 | 0.3677 |
Actin | 0.0049 | 0.0025 | 0.0015 | 0.0056 | 0.0008 | 0.0034 | 0.0197 | 0.0139 | 0.0073 | 0.0283 | 0.0034 | 0.0033 | 0.0551 | 0.0107 | 0.0635 | 0.0063 | 0.0203 | 0.0058 |
Bud | 0.0002 | 0.0002 | 0.0023 | 0.0001 | 0.0001 | 0.0001 | 0.0002 | 0.0004 | 0.0002 | 0.0001 | 0.0001 | 0.0002 | 0.0009 | 0.001 | 0.0052 | 0.0006 | 0.0002 | 0.0001 |
Bud Neck | 0.0007 | 0.0004 | 0.0003 | 0.0002 | 0.001 | 0.0006 | 0.0014 | 0.0003 | 0.0004 | 0.0009 | 0.0004 | 0.0007 | 0.002 | 0.0008 | 0.0013 | 0.0007 | 0.0003 | 0.0005 |
Bud Periphery | 0.0003 | 0.0001 | 0.0025 | 0.0002 | 0.0002 | 0.0001 | 0.0003 | 0.0008 | 0.0002 | 0.0002 | 0.0002 | 0.0004 | 0.0008 | 0.0006 | 0.0035 | 0.0007 | 0.0002 | 0.0001 |
Bud Site | 0.0013 | 0.0042 | 0.0007 | 0.0002 | 0.0004 | 0.0002 | 0.002 | 0.0011 | 0.0008 | 0.0007 | 0.0005 | 0.0002 | 0.0079 | 0.0071 | 0.0045 | 0.0032 | 0.0008 | 0.0003 |
Cell Periphery | 0.0002 | 0.0001 | 0.0001 | 0 | 0.0001 | 0 | 0.001 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0 | 0.0002 | 0.0001 | 0.0001 | 0.0001 | 0 | 0.0001 |
Cytoplasm | 0.0048 | 0.0029 | 0.0025 | 0.0013 | 0.0058 | 0.0016 | 0.0085 | 0.0095 | 0.0021 | 0.0006 | 0.0079 | 0.0026 | 0.0302 | 0.0047 | 0.0086 | 0.0014 | 0.0036 | 0.0022 |
Cytoplasmic Foci | 0.1963 | 0.2156 | 0.1776 | 0.1797 | 0.1834 | 0.1502 | 0.176 | 0.2079 | 0.192 | 0.151 | 0.1459 | 0.1473 | 0.1949 | 0.2636 | 0.215 | 0.2339 | 0.2709 | 0.1791 |
Eisosomes | 0.0005 | 0 | 0.0002 | 0.0001 | 0 | 0 | 0.0005 | 0.0001 | 0.0001 | 0.0013 | 0 | 0.0001 | 0.0006 | 0 | 0.0001 | 0.0001 | 0.0001 | 0.0001 |
Endoplasmic Reticulum | 0.0008 | 0.0002 | 0.0004 | 0.0002 | 0.0009 | 0.0003 | 0.0014 | 0.0003 | 0.0005 | 0.0001 | 0.0007 | 0.0005 | 0.004 | 0.0011 | 0.002 | 0.0004 | 0.0005 | 0.0005 |
Endosome | 0.1875 | 0.1927 | 0.1814 | 0.1874 | 0.2061 | 0.1396 | 0.1937 | 0.1709 | 0.1809 | 0.139 | 0.2236 | 0.1316 | 0.1765 | 0.2049 | 0.1968 | 0.2867 | 0.244 | 0.1839 |
Golgi | 0.1304 | 0.1636 | 0.1461 | 0.1672 | 0.1448 | 0.1402 | 0.1302 | 0.1869 | 0.1611 | 0.135 | 0.127 | 0.1657 | 0.128 | 0.1699 | 0.1696 | 0.2789 | 0.2397 | 0.1892 |
Lipid Particles | 0.1212 | 0.0916 | 0.0881 | 0.1011 | 0.1363 | 0.1036 | 0.1314 | 0.0821 | 0.0918 | 0.1736 | 0.1994 | 0.1145 | 0.1519 | 0.0635 | 0.0942 | 0.0453 | 0.0627 | 0.1396 |
Mitochondria | 0.031 | 0.0202 | 0.0339 | 0.023 | 0.0242 | 0.0466 | 0.0229 | 0.0422 | 0.0364 | 0.0268 | 0.0184 | 0.0329 | 0.0095 | 0.0096 | 0.0212 | 0.0303 | 0.0029 | 0.0694 |
None | 0.0186 | 0.0061 | 0.0153 | 0.0036 | 0.0048 | 0.0012 | 0.0132 | 0.0096 | 0.0044 | 0.0005 | 0.0006 | 0.0057 | 0.0133 | 0.0045 | 0.0035 | 0.0006 | 0.0778 | 0.0056 |
Nuclear Periphery | 0.0021 | 0.0001 | 0.0003 | 0.0002 | 0.0007 | 0.0002 | 0.0017 | 0.0004 | 0.0004 | 0.0001 | 0.0005 | 0.0008 | 0.0126 | 0.0006 | 0.0016 | 0.0007 | 0.0002 | 0.0003 |
Nucleolus | 0.0009 | 0.0003 | 0.0002 | 0.0001 | 0.0041 | 0.0001 | 0.0007 | 0.0007 | 0.0005 | 0.0003 | 0.001 | 0.0002 | 0.0006 | 0.0009 | 0.0004 | 0.0003 | 0.0001 | 0.0002 |
Nucleus | 0.0011 | 0.0001 | 0.0002 | 0 | 0.0006 | 0.0001 | 0.0007 | 0.0003 | 0.0005 | 0.0001 | 0.0004 | 0.0003 | 0.0072 | 0.0002 | 0.0004 | 0.0003 | 0.0001 | 0.0001 |
Peroxisomes | 0.2901 | 0.2957 | 0.3416 | 0.3282 | 0.2804 | 0.4086 | 0.2846 | 0.2662 | 0.3151 | 0.3388 | 0.2649 | 0.3895 | 0.1907 | 0.2497 | 0.2028 | 0.1031 | 0.0721 | 0.2181 |
Punctate Nuclear | 0.0038 | 0.0015 | 0.0014 | 0.0007 | 0.0012 | 0.001 | 0.0058 | 0.0016 | 0.002 | 0.0009 | 0.0021 | 0.0012 | 0.0082 | 0.0028 | 0.0018 | 0.0038 | 0.0024 | 0.0017 |
Vacuole | 0.0021 | 0.0012 | 0.0025 | 0.0005 | 0.0031 | 0.0011 | 0.0028 | 0.0036 | 0.0019 | 0.0009 | 0.0015 | 0.0012 | 0.003 | 0.0025 | 0.0025 | 0.0016 | 0.0006 | 0.0016 |
Vacuole Periphery | 0.0011 | 0.0006 | 0.001 | 0.0005 | 0.0012 | 0.0008 | 0.0011 | 0.0011 | 0.0011 | 0.0006 | 0.0012 | 0.001 | 0.0019 | 0.0009 | 0.0013 | 0.0012 | 0.0006 | 0.0015 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 19.0129 | 18.7546 | 10.0027 | 12.9878 | 14.4506 | 8.5183 | 12.7406 | 12.9986 | 9.4992 | 15.2669 |
Translational Efficiency | 0.336 | 0.4279 | 0.5767 | 0.4236 | 0.3435 | 0.7682 | 0.6208 | 0.365 | 0.5028 | 0.4058 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
24 | 1021 | 304 | 602 | 1361 | 1805 | 338 | 208 | 1385 | 2826 | 642 | 810 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 549.81 | 711.03 | 1023.17 | 915.94 | 760.34 | 761.08 | 985.74 | 899.36 | 756.69 | 743.00 | 1003.46 | 911.68 |
Standard Deviation | 55.43 | 135.44 | 115.58 | 120.94 | 85.18 | 100.40 | 101.52 | 102.31 | 89.10 | 116.81 | 110.00 | 116.67 |
Intensity Change Log 2 | — | 0.370977 | 0.896041 | 0.736320 | — | 0.001403 | 0.374562 | 0.242254 | — | 0.168153 | 0.616681 | 0.470476 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.000321 | 0.001448 | 0.028222 | 0.014532 | 0.001127 | 0.001301 | 0.005126 | 0.004727 | 0.001113 | 0.001354 | 0.016062 | 0.012014 |
Bud Neck | 0.003210 | 0.003038 | 0.006097 | 0.005787 | 0.002145 | 0.002153 | 0.003650 | 0.010503 | 0.002163 | 0.002473 | 0.004808 | 0.006998 |
Bud Site | 0.000270 | 0.005152 | 0.037886 | 0.029742 | 0.001904 | 0.002706 | 0.009620 | 0.012650 | 0.001876 | 0.003590 | 0.023005 | 0.025353 |
Cell Periphery | 0.000187 | 0.000066 | 0.000187 | 0.000049 | 0.000049 | 0.000052 | 0.000061 | 0.000042 | 0.000052 | 0.000057 | 0.000121 | 0.000047 |
Cytoplasm | 0.037541 | 0.010187 | 0.019953 | 0.016287 | 0.008599 | 0.004960 | 0.015624 | 0.014676 | 0.009101 | 0.006849 | 0.017674 | 0.015873 |
Cytoplasmic Foci | 0.363934* | 0.297353* | 0.261112* | 0.291271* | 0.327224* | 0.290750* | 0.368151* | 0.230062* | 0.327860* | 0.293135* | 0.317465* | 0.275553* |
Eisosomes | 0.000354 | 0.000085 | 0.000120 | 0.000052 | 0.000102 | 0.000076 | 0.000075 | 0.000085 | 0.000106 | 0.000079 | 0.000096 | 0.000061 |
Endoplasmic Reticulum | 0.000230 | 0.000212 | 0.007687 | 0.000596 | 0.000568 | 0.000171 | 0.001535 | 0.001331 | 0.000562 | 0.000186 | 0.004448 | 0.000785 |
Endosome | 0.092122 | 0.191559 | 0.121851 | 0.220695* | 0.114260 | 0.176369 | 0.212117* | 0.271262* | 0.113876 | 0.181857 | 0.169374 | 0.233680* |
Golgi | 0.116004* | 0.313642* | 0.056829 | 0.053457 | 0.400272* | 0.369348* | 0.053784 | 0.029450 | 0.395346* | 0.349222* | 0.055226 | 0.047292 |
Lipid Particles | 0.080069 | 0.026671 | 0.036254 | 0.012719 | 0.023346 | 0.022834 | 0.041249 | 0.019201 | 0.024329 | 0.024220 | 0.038884 | 0.014384 |
Mitochondria | 0.067382 | 0.026156 | 0.001296 | 0.001151 | 0.035011 | 0.034539 | 0.001234 | 0.008068 | 0.035572 | 0.031510 | 0.001263 | 0.002927 |
Mitotic Spindle | 0.000690 | 0.019391 | 0.274566* | 0.138999* | 0.011276 | 0.012144 | 0.123281* | 0.156386* | 0.011093 | 0.014762 | 0.194917* | 0.143464* |
None | 0.000571 | 0.000504 | 0.000081 | 0.000145 | 0.000193 | 0.000163 | 0.000106 | 0.000075 | 0.000200 | 0.000286 | 0.000094 | 0.000127 |
Nuclear Periphery | 0.000184 | 0.000295 | 0.000693 | 0.000201 | 0.000079 | 0.000046 | 0.000535 | 0.001012 | 0.000081 | 0.000136 | 0.000610 | 0.000409 |
Nuclear Periphery Foci | 0.000139 | 0.001812 | 0.014731 | 0.001975 | 0.000453 | 0.000408 | 0.006775 | 0.005552 | 0.000448 | 0.000915 | 0.010542 | 0.002894 |
Nucleolus | 0.000285 | 0.001741 | 0.000451 | 0.000495 | 0.000549 | 0.000423 | 0.000969 | 0.001237 | 0.000544 | 0.000899 | 0.000724 | 0.000685 |
Nucleus | 0.018520 | 0.003540 | 0.004184 | 0.000871 | 0.000788 | 0.000656 | 0.001595 | 0.004372 | 0.001095 | 0.001698 | 0.002821 | 0.001770 |
Peroxisomes | 0.215421* | 0.089319 | 0.057461 | 0.154397* | 0.065419 | 0.074634 | 0.106210* | 0.164114* | 0.068018 | 0.079939 | 0.083126 | 0.156892* |
Vacuole | 0.001130 | 0.005026 | 0.068627 | 0.054270 | 0.004504 | 0.004247 | 0.047379 | 0.062832 | 0.004446 | 0.004528 | 0.057441 | 0.056469 |
Vacuole Periphery | 0.001436 | 0.002806 | 0.001714 | 0.002307 | 0.002132 | 0.002021 | 0.000925 | 0.002363 | 0.002119 | 0.002304 | 0.001299 | 0.002322 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -4.71 | -5.92 | -5.34 | -4.87 | 2.74 | -0.65 | -4.98 | -1.10 | -1.04 | 0.38 | -1.04 | -6.48 | -5.05 | -4.93 | 1.63 |
Bud Neck | 0.06 | -2.63 | -2.29 | -3.45 | 0.52 | -0.01 | -3.09 | -2.92 | -2.90 | -2.17 | -0.67 | -5.74 | -5.42 | -5.00 | -1.85 |
Bud Site | -5.51 | -9.76 | -9.04 | -7.45 | 1.16 | -1.46 | -5.94 | -5.68 | -5.33 | -2.22 | -3.44 | -10.52 | -9.72 | -9.01 | -1.37 |
Cell Periphery | 1.79 | -0.34 | 1.97 | 1.35 | 8.38 | -0.17 | -1.73 | -0.62 | -0.53 | 0.89 | -0.50 | -6.28 | -0.38 | 0.17 | 7.18 |
Cytoplasm | 1.41 | 0.62 | 0.53 | -4.41 | -0.35 | 3.17 | -2.80 | -3.27 | -4.08 | -0.76 | 1.82 | -4.36 | -5.72 | -6.75 | -0.96 |
Cytoplasmic Foci | 1.33 | 2.30 | 1.86 | 2.86 | -1.72 | 5.92 | -1.70 | 8.80 | 5.98 | 8.01 | 6.09 | 2.92 | 8.60 | 4.89 | 3.98 |
Eisosomes | 3.79 | 3.17 | 4.23 | 4.16 | 6.20 | 3.54 | 2.13 | 0.14 | -0.75 | -0.51 | 3.83 | 0.34 | 3.98 | 1.51 | 3.65 |
Endoplasmic Reticulum | 0.06 | -6.94 | -2.57 | -3.88 | 6.51 | 1.50 | -3.37 | -1.37 | -2.11 | 0.63 | 1.46 | -6.93 | -1.27 | -4.01 | 6.54 |
Endosome | -4.02 | -1.11 | -4.31 | -1.02 | -7.47 | -10.69 | -7.77 | -7.58 | -3.84 | -2.04 | -13.03 | -6.27 | -11.24 | -3.53 | -4.85 |
Golgi | -6.95 | 2.25 | 2.50 | 32.91* | 1.02 | 3.44 | 44.44* | 47.49* | 49.40* | 4.28 | 5.67 | 45.99* | 48.86* | 58.74* | 2.40 |
Lipid Particles | 1.78 | 1.45 | 2.26 | 4.13 | 5.41 | 0.23 | -3.16 | 0.92 | 0.78 | 3.50 | 0.06 | -3.72 | 3.52 | 4.08 | 6.55 |
Mitochondria | 1.63 | 2.66 | 2.67 | 9.72 | 0.36 | 0.16 | 13.66 | 6.13 | 6.06 | -1.38 | 1.37 | 13.94 | 12.43 | 14.06 | -1.24 |
Mitotic Spindle | -7.50 | -14.99 | -13.65 | -11.51 | 6.00 | -0.46 | -9.73 | -8.07 | -8.03 | -1.48 | -2.12 | -16.73 | -14.88 | -14.50 | 3.24 |
None | -0.02 | 1.45 | 0.66 | 0.87 | -0.84 | 0.54 | 0.53 | 2.22 | 0.84 | 0.66 | -1.14 | 1.38 | -0.26 | 0.29 | -0.68 |
Nuclear Periphery | -0.65 | -3.52 | -1.04 | -0.07 | 3.32 | 1.11 | -2.69 | -2.23 | -2.32 | -0.19 | -0.88 | -4.49 | -3.43 | -2.83 | 2.03 |
Nuclear Periphery Foci | -2.83 | -6.59 | -5.88 | -1.23 | 5.36 | 0.29 | -3.60 | -4.06 | -4.09 | 0.43 | -1.83 | -6.73 | -6.03 | -5.08 | 4.35 |
Nucleolus | -2.63 | -1.57 | -1.97 | 2.03 | -0.55 | 0.73 | -1.60 | -2.70 | -3.16 | 0.32 | -1.46 | -1.52 | -1.65 | 0.24 | 0.79 |
Nucleus | 0.90 | 0.73 | 0.98 | 0.98 | 2.83 | 0.39 | -2.29 | -2.48 | -2.53 | -1.16 | -0.97 | -3.95 | -2.84 | -1.88 | 1.37 |
Peroxisomes | 2.45 | 3.10 | 1.46 | -5.54 | -8.38 | -1.85 | -3.59 | -5.68 | -5.10 | -2.92 | -2.58 | -1.59 | -9.63 | -8.50 | -7.15 |
Vacuole | -5.65 | -10.01 | -11.57 | -11.04 | -0.70 | 0.07 | -8.36 | -7.46 | -7.47 | -1.94 | -0.27 | -12.64 | -13.31 | -13.30 | -2.02 |
Vacuole Periphery | -1.80 | -0.60 | -0.73 | 0.82 | -0.40 | 0.33 | 5.04 | 0.20 | 0.05 | -1.49 | -0.53 | 3.27 | 0.16 | 0.44 | -1.25 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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