Standard name | |
---|---|
Human Ortholog | |
Description | Component of glucose deprivation induced stress granules; involved in P-body-dependent granule assembly; similar to human ataxin-2; interacts with Pab1p to regulate mRNA polyadenylation; interacts with Mkt1p to regulate HO translation; protein increases in abundance and relative distribution to the nucleus increases upon DNA replication stress |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 1 | 0 | 0 | 1 | 2 | 2 | 1 | 2 | – | 2 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 5 |
Bud | 0 | 0 | 0 | 0 | 0 | 1 | 2 | 1 | 3 | – | 9 | 3 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 1 | 3 | 0 | 1 | 2 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 4 | 2 | 0 | 1 | 0 | 0 | 0 | – | 2 | 1 | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 323 | 443 | 335 | 155 | 109 | 143 | 122 | 71 | 67 | – | 73 | 55 | 113 | 182 | 83 | 14 | 12 | 22 | 302 | 394 | 371 | 91 | 153 | 119 |
Endoplasmic Reticulum | 1 | 13 | 10 | 4 | 13 | 3 | 9 | 2 | 6 | – | 3 | 1 | 2 | 2 | 1 | 1 | 0 | 0 | 1 | 4 | 6 | 3 | 6 | 5 |
Endosome | 3 | 0 | 0 | 1 | 0 | 2 | 8 | 3 | 5 | – | 2 | 1 | 1 | 0 | 1 | 0 | 1 | 0 | 2 | 7 | 6 | 0 | 1 | 5 |
Golgi | 0 | 1 | 0 | 0 | 0 | 5 | 3 | 3 | 1 | – | 0 | 1 | 0 | 2 | 0 | 1 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 2 |
Mitochondria | 1 | 1 | 0 | 3 | 1 | 15 | 24 | 6 | 69 | – | 96 | 29 | 1 | 0 | 1 | 1 | 0 | 0 | 1 | 1 | 4 | 1 | 0 | 1 |
Nucleus | 4 | 4 | 19 | 27 | 55 | 123 | 176 | 161 | 129 | – | 171 | 127 | 2 | 4 | 9 | 2 | 0 | 0 | 38 | 61 | 75 | 3 | 2 | 3 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | – | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 3 | 2 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 6 | 8 | – | 14 | 3 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 1 |
Vac/Vac Membrane | 66 | 68 | 167 | 106 | 150 | 180 | 170 | 167 | 118 | – | 124 | 123 | 55 | 137 | 119 | 6 | 15 | 16 | 9 | 29 | 54 | 12 | 15 | 13 |
Unique Cell Count | 373 | 493 | 456 | 256 | 291 | 416 | 483 | 387 | 360 | 442 | 316 | 158 | 292 | 195 | 21 | 24 | 33 | 368 | 513 | 533 | 116 | 189 | 167 | |
Labelled Cell Count | 398 | 531 | 535 | 298 | 329 | 475 | 516 | 423 | 410 | 496 | 346 | 174 | 328 | 215 | 26 | 28 | 39 | 368 | 513 | 533 | 116 | 189 | 167 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 11.4 | 13.6 | 10.4 | 10.0 | 9.9 | 8.9 | 8.7 | 9.2 | 8.2 | – | 7.4 | 8.4 | 11.7 | 12.5 | 12.8 | 18.5 | 18.0 | 16.9 | 10.6 | 11.4 | 12.9 |
Std Deviation (1e-4) | 1.9 | 2.1 | 1.4 | 1.6 | 1.5 | 1.5 | 1.5 | 1.7 | 1.6 | – | 1.5 | 1.7 | 2.0 | 1.9 | 2.3 | 2.5 | 4.7 | 3.5 | 2.8 | 1.9 | 2.6 |
Intensity Change (Log2) | – | – | – | -0.05 | -0.06 | -0.22 | -0.26 | -0.18 | -0.34 | – | -0.48 | -0.31 | 0.17 | 0.27 | 0.3 | 0.83 | 0.79 | 0.7 | 0.02 | 0.13 | 0.32 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | -3.6 | -9.8 | -11.6 | -14.8 | -16.0 | -15.6 | – | -17.1 | -15.3 | -0.5 | -3.2 | -7.5 | -0.7 | -2.5 | -0.8 |
Endoplasmic Reticulum | -0.6 | 1.8 | -1.8 | -0.4 | -2.0 | -0.5 | – | -1.9 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 4.1 | 4.8 | 0 | 9.8 | – | 10.5 | 6.6 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 3.3 | 6.6 | 10.1 | 12.2 | 13.2 | 11.7 | – | 12.7 | 12.6 | -1.7 | -2.2 | 0.3 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | – | 3.8 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 1.3 | 4.0 | 2.0 | -0.5 | 1.9 | -1.1 | – | -2.7 | 0.6 | -0.4 | 2.8 | 5.7 | -0.8 | 2.5 | 1.4 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 5.683 | 8.1124 | 6.8905 | 6.4069 | 5.9784 | 7.0174 | 7.7785 | 10.5924 | 8.9991 | 9.3711 | 8.4312 | 9.3534 | 8.1582 | 9.9227 | 9.4182 | 8.7174 | 8.2653 | 9.1172 |
Actin | 0.014 | 0.0004 | 0.0012 | 0.0162 | 0.0009 | 0.0041 | 0.0348 | 0.0002 | 0.0138 | 0.0166 | 0.0336 | 0.0003 | 0.0023 | 0.0008 | 0.0149 | 0.0025 | 0.0112 | 0.001 |
Bud | 0.0006 | 0.0002 | 0.0002 | 0.0003 | 0.0021 | 0.0003 | 0.0008 | 0.0002 | 0.0003 | 0.0004 | 0.0005 | 0.0004 | 0.0003 | 0.0003 | 0.0001 | 0.0003 | 0.0002 | 0.0001 |
Bud Neck | 0.0016 | 0.0004 | 0.0006 | 0.0009 | 0.0012 | 0.0019 | 0.0071 | 0.0003 | 0.0013 | 0.0009 | 0.0018 | 0.0016 | 0.0008 | 0.0006 | 0.0005 | 0.0009 | 0.0009 | 0.0018 |
Bud Periphery | 0.0004 | 0 | 0.0001 | 0.0003 | 0.0032 | 0.0002 | 0.0009 | 0 | 0.0003 | 0.0004 | 0.0008 | 0.0002 | 0.0002 | 0.0001 | 0.0001 | 0.0001 | 0.0003 | 0 |
Bud Site | 0.0012 | 0.0004 | 0.0004 | 0.0011 | 0.0016 | 0.0003 | 0.0028 | 0.0007 | 0.0007 | 0.0012 | 0.0009 | 0.0002 | 0.0004 | 0.0007 | 0.0003 | 0.0022 | 0.0004 | 0.0001 |
Cell Periphery | 0.0001 | 0 | 0.0001 | 0.0001 | 0.0003 | 0 | 0.0002 | 0 | 0.0001 | 0.0001 | 0.0001 | 0 | 0.0001 | 0 | 0 | 0 | 0.0001 | 0 |
Cytoplasm | 0.4722 | 0.5348 | 0.4421 | 0.4132 | 0.4536 | 0.4299 | 0.449 | 0.5392 | 0.4122 | 0.2762 | 0.4616 | 0.4426 | 0.4619 | 0.5797 | 0.4538 | 0.4704 | 0.5321 | 0.4602 |
Cytoplasmic Foci | 0.0171 | 0.0044 | 0.0147 | 0.0113 | 0.0048 | 0.0061 | 0.0269 | 0.0023 | 0.0039 | 0.0013 | 0.0051 | 0.002 | 0.0143 | 0.0011 | 0.0012 | 0.0178 | 0.009 | 0.0037 |
Eisosomes | 0.0001 | 0 | 0.0001 | 0.0001 | 0 | 0 | 0.0002 | 0 | 0.0001 | 0.0002 | 0.0003 | 0 | 0 | 0 | 0.0001 | 0 | 0.0005 | 0 |
Endoplasmic Reticulum | 0.0093 | 0.0025 | 0.0044 | 0.0024 | 0.0063 | 0.0039 | 0.0079 | 0.0007 | 0.0041 | 0.0062 | 0.0055 | 0.0027 | 0.0097 | 0.0016 | 0.0014 | 0.0013 | 0.0215 | 0.003 |
Endosome | 0.0494 | 0.0014 | 0.0096 | 0.0176 | 0.0488 | 0.0109 | 0.0475 | 0.0005 | 0.0081 | 0.0106 | 0.0394 | 0.0053 | 0.0144 | 0.001 | 0.007 | 0.0125 | 0.0157 | 0.0052 |
Golgi | 0.0099 | 0.0001 | 0.0009 | 0.0108 | 0.0033 | 0.0031 | 0.018 | 0.0001 | 0.0037 | 0.0016 | 0.0118 | 0.001 | 0.0026 | 0.0001 | 0.0035 | 0.0041 | 0.0022 | 0.0009 |
Lipid Particles | 0.0115 | 0 | 0.0017 | 0.0159 | 0.0014 | 0.0056 | 0.0246 | 0.0002 | 0.0011 | 0.0009 | 0.0074 | 0.0004 | 0.0034 | 0 | 0.002 | 0.0021 | 0.0044 | 0.0004 |
Mitochondria | 0.0086 | 0.0003 | 0.011 | 0.0128 | 0.0131 | 0.0016 | 0.0071 | 0.0002 | 0.0089 | 0.0021 | 0.0038 | 0.0017 | 0.0029 | 0.0006 | 0.0042 | 0.0005 | 0.0098 | 0.0036 |
None | 0.0052 | 0.0007 | 0.0015 | 0.0028 | 0.0014 | 0.0008 | 0.0034 | 0.0006 | 0.0008 | 0.0023 | 0.0011 | 0.0004 | 0.0025 | 0.0007 | 0.0006 | 0.0012 | 0.0021 | 0.0006 |
Nuclear Periphery | 0.0332 | 0.0209 | 0.0223 | 0.0166 | 0.0284 | 0.0321 | 0.0425 | 0.0116 | 0.0247 | 0.0269 | 0.0304 | 0.022 | 0.03 | 0.013 | 0.0127 | 0.0124 | 0.0274 | 0.0175 |
Nucleolus | 0.001 | 0.0002 | 0.0003 | 0.0016 | 0.0006 | 0.0008 | 0.0012 | 0.0007 | 0.0003 | 0.0005 | 0.0006 | 0.0003 | 0.0007 | 0.0002 | 0.0002 | 0.0002 | 0.0004 | 0.0002 |
Nucleus | 0.3443 | 0.4287 | 0.4764 | 0.4494 | 0.3991 | 0.4782 | 0.3006 | 0.438 | 0.5084 | 0.6455 | 0.3806 | 0.5094 | 0.4392 | 0.3965 | 0.4906 | 0.4632 | 0.3482 | 0.4967 |
Peroxisomes | 0.0032 | 0.0002 | 0.0058 | 0.0185 | 0.0004 | 0.0006 | 0.0086 | 0.0001 | 0.0004 | 0.0004 | 0.0013 | 0.0003 | 0.0011 | 0.0001 | 0.0016 | 0.0034 | 0.0015 | 0.0003 |
Punctate Nuclear | 0.0081 | 0.0019 | 0.0027 | 0.0034 | 0.0025 | 0.0156 | 0.007 | 0.0036 | 0.0046 | 0.0031 | 0.0058 | 0.0058 | 0.0091 | 0.0018 | 0.0016 | 0.0039 | 0.009 | 0.0027 |
Vacuole | 0.0069 | 0.0019 | 0.0029 | 0.0035 | 0.0201 | 0.0032 | 0.0057 | 0.0006 | 0.0014 | 0.0017 | 0.0058 | 0.0026 | 0.0032 | 0.0009 | 0.0025 | 0.0008 | 0.0021 | 0.0014 |
Vacuole Periphery | 0.0021 | 0.0004 | 0.001 | 0.0013 | 0.0071 | 0.0009 | 0.003 | 0.0001 | 0.0007 | 0.001 | 0.0019 | 0.0008 | 0.001 | 0.0002 | 0.0011 | 0.0002 | 0.001 | 0.0004 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 77.5792 | 90.0426 | 80.9885 | 86.8597 | 87.0635 | 77.282 | 90.6972 | 99.9293 | 108.1315 | 89.4948 |
Translational Efficiency | 1.5842 | 1.4405 | 1.4344 | 1.5673 | 1.3359 | 1.6694 | 1.3324 | 1.309 | 1.2058 | 1.3463 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
1621 | 1434 | 1689 | 1127 | 1695 | 1855 | 1338 | 236 | 3316 | 3289 | 3027 | 1363 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 915.58 | 1110.16 | 1852.88 | 1462.04 | 1017.16 | 1106.35 | 1466.75 | 1676.95 | 967.50 | 1108.01 | 1682.20 | 1499.25 |
Standard Deviation | 128.29 | 182.42 | 277.15 | 256.80 | 194.72 | 158.13 | 222.29 | 276.36 | 173.22 | 169.16 | 318.55 | 272.70 |
Intensity Change Log 2 | — | 0.278010 | 1.017012 | 0.675225 | — | 0.121261 | 0.528076 | 0.721293 | — | 0.197642 | 0.780375 | 0.699653 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.000318 | 0.000569 | 0.000022 | 0.000560 | 0.000409 | 0.000539 | 0.000479 | 0.001114 | 0.000364 | 0.000552 | 0.000224 | 0.000656 |
Bud Neck | 0.001887 | 0.003426 | 0.001711 | 0.014164 | 0.001976 | 0.002399 | 0.003574 | 0.006507 | 0.001932 | 0.002847 | 0.002534 | 0.012838 |
Bud Site | 0.003694 | 0.003581 | 0.000360 | 0.010053 | 0.002588 | 0.004845 | 0.002058 | 0.009060 | 0.003129 | 0.004294 | 0.001110 | 0.009881 |
Cell Periphery | 0.000135 | 0.000167 | 0.000014 | 0.000269 | 0.000140 | 0.000077 | 0.000152 | 0.000342 | 0.000138 | 0.000116 | 0.000075 | 0.000281 |
Cytoplasm | 0.512800* | 0.408051* | 0.533956* | 0.195297* | 0.408592* | 0.497240* | 0.205634* | 0.148798* | 0.459534* | 0.458354* | 0.388831* | 0.187246* |
Cytoplasmic Foci | 0.015328 | 0.006005 | 0.000270 | 0.002294 | 0.006910 | 0.012819 | 0.001482 | 0.016281 | 0.011025 | 0.009848 | 0.000806 | 0.004716 |
Eisosomes | 0.000011 | 0.000012 | 0.000001 | 0.000010 | 0.000012 | 0.000012 | 0.000010 | 0.000026 | 0.000011 | 0.000012 | 0.000005 | 0.000013 |
Endoplasmic Reticulum | 0.002971 | 0.003886 | 0.000111 | 0.001160 | 0.005103 | 0.002576 | 0.002227 | 0.003476 | 0.004061 | 0.003147 | 0.001046 | 0.001561 |
Endosome | 0.000538 | 0.000877 | 0.000045 | 0.002436 | 0.001110 | 0.002731 | 0.000673 | 0.017965 | 0.000831 | 0.001923 | 0.000323 | 0.005125 |
Golgi | 0.000183 | 0.000335 | 0.000023 | 0.002252 | 0.000960 | 0.002348 | 0.000130 | 0.072173 | 0.000580 | 0.001470 | 0.000070 | 0.014358 |
Lipid Particles | 0.000404 | 0.000534 | 0.000048 | 0.000330 | 0.000678 | 0.000988 | 0.000459 | 0.002314 | 0.000544 | 0.000790 | 0.000230 | 0.000674 |
Mitochondria | 0.001135 | 0.001438 | 0.000509 | 0.002053 | 0.000678 | 0.003001 | 0.001247 | 0.004332 | 0.000902 | 0.002320 | 0.000835 | 0.002447 |
Mitotic Spindle | 0.000603 | 0.005880 | 0.002647 | 0.045188 | 0.004669 | 0.016099 | 0.022892 | 0.012820 | 0.002681 | 0.011643 | 0.011596 | 0.039583 |
None | 0.011546 | 0.007655 | 0.009393 | 0.015442 | 0.009806 | 0.006952 | 0.009786 | 0.014087 | 0.010656 | 0.007258 | 0.009567 | 0.015207 |
Nuclear Periphery | 0.000378 | 0.000873 | 0.000893 | 0.001401 | 0.001066 | 0.000856 | 0.001788 | 0.001889 | 0.000730 | 0.000864 | 0.001289 | 0.001485 |
Nuclear Periphery Foci | 0.001770 | 0.003042 | 0.000094 | 0.000886 | 0.003952 | 0.002771 | 0.001476 | 0.004132 | 0.002885 | 0.002889 | 0.000705 | 0.001448 |
Nucleolus | 0.000571 | 0.000408 | 0.000164 | 0.001286 | 0.000590 | 0.000974 | 0.000351 | 0.000315 | 0.000581 | 0.000728 | 0.000247 | 0.001118 |
Nucleus | 0.430340* | 0.534526* | 0.447404* | 0.689040* | 0.534797* | 0.419737* | 0.724140* | 0.641444* | 0.483734* | 0.469785* | 0.569727* | 0.680799* |
Peroxisomes | 0.000174 | 0.000423 | 0.000008 | 0.000133 | 0.000377 | 0.000968 | 0.000058 | 0.000954 | 0.000278 | 0.000731 | 0.000030 | 0.000275 |
Vacuole | 0.014736 | 0.018133 | 0.002266 | 0.013000 | 0.015324 | 0.020816 | 0.021278 | 0.024371 | 0.015037 | 0.019646 | 0.010670 | 0.014969 |
Vacuole Periphery | 0.000477 | 0.000179 | 0.000062 | 0.002745 | 0.000264 | 0.001252 | 0.000106 | 0.017601 | 0.000368 | 0.000784 | 0.000082 | 0.005318 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -5.11 | 11.29 | -8.10 | -2.00 | -14.06 | -0.94 | -6.19 | -5.68 | -2.58 | -2.62 | -2.51 | 0.71 | -8.97 | -2.42 | -9.48 |
Bud Neck | -4.68 | -0.24 | -10.05 | -7.78 | -9.73 | -1.87 | -10.55 | -8.70 | -8.29 | -5.54 | -4.95 | -7.19 | -11.72 | -10.31 | -9.73 |
Bud Site | -0.65 | 6.73 | -2.61 | -2.29 | -5.62 | -2.54 | -1.15 | -2.48 | -1.56 | -2.29 | -2.45 | 4.32 | -3.76 | -2.35 | -5.32 |
Cell Periphery | -2.53 | 10.77 | -7.92 | -5.72 | -16.64 | 6.17 | -4.35 | -7.63 | -9.41 | -6.15 | 1.88 | 4.94 | -10.64 | -11.99 | -13.84 |
Cytoplasm | 12.14 | 3.86 | 43.89 | 29.19 | 44.45* | -8.71 | 30.30 | 20.89 | 27.20 | 2.44 | 2.02 | 17.74 | 48.30 | 46.71 | 30.44 |
Cytoplasmic Foci | 6.52 | 12.16 | 10.91 | 8.08 | -5.60 | -5.43 | 9.36 | 2.04 | 5.97 | -3.10 | 1.24 | 15.14 | 12.47 | 12.63 | -4.63 |
Eisosomes | -2.63 | 12.41 | -8.85 | -6.23 | -19.17 | -0.99 | -5.21 | -7.90 | -7.55 | -6.18 | -2.53 | 4.57 | -11.61 | -9.77 | -14.84 |
Endoplasmic Reticulum | -4.59 | 17.13 | 4.73 | 8.56 | -17.10 | 6.49 | 5.48 | 5.28 | 0.30 | 1.25 | 2.90 | 12.56 | 8.86 | 7.38 | -5.00 |
Endosome | -3.77 | 11.86 | -0.78 | 0.52 | -2.31 | -3.37 | 1.69 | -1.23 | 0.14 | -1.49 | -4.18 | 5.28 | -0.72 | 2.03 | -2.06 |
Golgi | -1.19 | 6.74 | -1.55 | -0.96 | -2.14 | -1.82 | 1.99 | -1.60 | -1.32 | -1.77 | -2.12 | 2.42 | -1.60 | -0.63 | -2.22 |
Lipid Particles | -3.77 | 6.21 | -5.42 | -1.46 | -13.84 | -1.80 | -1.11 | -3.65 | -2.04 | -3.40 | -2.89 | 2.34 | -4.76 | -0.43 | -8.26 |
Mitochondria | -0.25 | 0.65 | -2.25 | -1.80 | -11.47 | -3.07 | -9.21 | -6.11 | 0.38 | -2.47 | -2.23 | -0.98 | -4.87 | -0.77 | -9.48 |
Mitotic Spindle | -4.31 | -4.30 | -8.15 | -6.07 | -6.75 | -4.31 | -6.47 | -3.09 | -1.21 | 0.63 | -5.88 | -7.18 | -8.15 | -5.00 | -4.00 |
None | 3.17 | 1.23 | -1.01 | -5.92 | -3.53 | 2.16 | 1.16 | -1.65 | -3.11 | -2.50 | 3.75 | 1.57 | -2.23 | -6.88 | -4.71 |
Nuclear Periphery | -13.18 | -12.86 | -20.33 | -14.65 | -12.18 | 1.92 | -15.28 | -7.22 | -7.92 | -0.43 | -4.50 | -17.99 | -18.71 | -17.08 | -7.45 |
Nuclear Periphery Foci | -3.58 | 10.82 | 6.45 | 5.79 | -7.33 | 1.38 | 5.28 | 4.42 | 3.04 | 0.39 | -1.27 | 8.91 | 8.42 | 7.61 | -0.55 |
Nucleolus | 0.39 | 1.73 | -2.05 | -2.82 | -4.12 | -1.98 | 0.78 | -1.38 | 1.35 | -2.57 | -1.50 | 2.08 | -2.44 | -0.99 | -3.93 |
Nucleus | -10.42 | -7.63 | -31.02 | -19.75 | -26.40 | 11.72 | -20.60 | -7.74 | -13.34 | 2.16 | 1.35 | -17.09 | -29.66 | -30.91 | -15.77 |
Peroxisomes | -1.16 | 5.50 | 1.12 | 1.43 | -3.04 | -2.02 | 1.71 | 0.76 | 2.70 | -1.39 | -2.41 | 2.72 | 1.47 | 3.38 | -2.70 |
Vacuole | -4.87 | 9.21 | -8.14 | -3.62 | -13.37 | -4.63 | -9.43 | -6.62 | -5.61 | -3.75 | -6.74 | -3.78 | -10.39 | -6.45 | -7.99 |
Vacuole Periphery | 1.52 | 2.16 | -0.43 | -1.26 | -1.58 | -2.15 | 2.25 | -1.22 | -0.59 | -1.30 | -1.45 | 2.78 | -1.29 | -0.35 | -1.99 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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