Standard name
Human Ortholog
Description Mitochondrial NADH-cytochrome b5 reductase; involved in ergosterol biosynthesis
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0.07 0.06 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0
Bud 0 0 0 0.06 0.08 0 0.07 0.05 0 0.06 0.05 0 0 0 0 0 0 – 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0
Cytoplasm 0.37 0.5 0.73 0.43 0.27 0.16 0.18 0.12 0.07 0.08 0.08 0.05 0.69 0.73 0.78 0.79 0.53 – 0.06 0.1 0.09 0.19 0.18 0.2
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0.08 0 0 0.07 0 – 0 0 0 0 0 0
Endosome 0.08 0 0 0 0 0.1 0 0.06 0 0 0 0 0 0.08 0.12 0 0.14 – 0.1 0.08 0.07 0.06 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0
Mitochondria 0.4 0.36 0 0.39 0.57 0.63 0.76 0.59 0.91 0.83 0.88 0.88 0.06 0.06 0 0.14 0.14 – 0.65 0.59 0.68 0.52 0.51 0.53
Nucleus 0 0 0 0 0 0 0.07 0.05 0 0 0 0 0 0 0 0.07 0 – 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0
SpindlePole 0.06 0 0.08 0.07 0.07 0.08 0.09 0.16 0 0.1 0.05 0.09 0 0.05 0 0.14 0.08 – 0 0 0 0 0.06 0
Vac/Vac Membrane 0.14 0.08 0.05 0 0 0.11 0 0.09 0 0.05 0.06 0 0.07 0 0 0 0.31 – 0.05 0.07 0 0.06 0.06 0.07
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 5 7 6 11 8 1 0 2 2 1 2 4 3 3 3 0 0 – 10 21 9 4 3 6
Bud 8 10 4 9 11 3 20 14 11 24 25 21 2 2 1 0 0 – 4 5 1 2 3 5
Bud Neck 3 1 6 1 5 2 4 8 3 2 0 2 0 0 0 0 0 – 3 10 2 1 4 6
Bud Site 2 1 0 0 1 3 4 1 10 4 2 6 0 0 0 1 0 – – – – – – –
Cell Periphery 8 11 7 4 0 0 0 2 1 5 2 6 1 6 5 0 0 – 1 4 1 0 0 0
Cytoplasm 109 211 121 65 36 52 48 35 26 34 35 25 87 127 178 22 19 – 25 63 25 35 33 46
Endoplasmic Reticulum 7 12 6 6 6 6 3 2 8 4 1 3 10 7 4 2 0 – 3 7 3 2 0 2
Endosome 24 13 5 5 2 32 2 18 3 11 5 9 5 14 27 1 5 – 44 51 20 10 8 8
Golgi 0 0 0 1 0 0 0 1 1 0 0 3 2 1 2 0 0 – 5 9 1 2 4 2
Mitochondria 118 151 6 60 76 199 202 170 317 345 400 485 8 10 4 4 5 – 286 362 189 95 96 126
Nucleus 2 1 1 1 2 11 18 15 11 10 14 10 0 1 0 2 1 – 0 1 1 0 2 0
Nuclear Periphery 0 1 0 0 0 1 1 0 3 11 4 10 0 0 1 0 0 – 1 1 0 0 1 0
Nucleolus 0 1 0 0 0 1 4 2 4 6 6 7 0 0 0 0 0 – 2 2 0 1 1 0
Peroxisomes 2 1 5 3 0 3 0 2 0 0 3 2 0 0 8 0 1 – 2 4 2 2 0 1
SpindlePole 19 12 14 10 10 26 23 45 15 40 24 47 3 9 5 4 3 – 16 12 7 6 10 5
Vac/Vac Membrane 40 33 8 1 3 34 6 25 9 19 27 22 9 6 10 0 11 – 24 44 4 11 10 16
Unique Cell Count 293 418 166 152 134 316 267 289 348 418 455 552 126 173 228 28 36 443 612 280 183 190 236
Labelled Cell Count 347 466 189 177 160 374 335 342 424 516 550 662 130 186 248 36 45 443 612 280 183 190 236


Mitochondria

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.6 4.1 5.2 4.9 4.5 4.8 3.8 5.2 4.0 4.7 4.3 4.6 5.5 5.7 5.3 5.0 5.9 – 6.4 6.1 6.8
Std Deviation (1e-4) 0.6 0.8 1.0 1.2 1.2 1.3 1.3 1.5 0.9 1.1 1.1 1.1 0.9 1.2 1.3 1.8 1.8 – 5.2 3.4 4.0
Intensity Change (Log2) – – – -0.08 -0.2 -0.11 -0.46 -0.0 -0.37 -0.16 -0.26 -0.18 0.08 0.12 0.03 -0.05 0.17 – 0.29 0.24 0.39


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 3.5558 3.4302 3.4554 3.341 3.8762 3.6996 7.8105 7.8319 7.2404 7.016 6.9462 7.6527 3.3524 4.1519 3.3853 4.03 3.6545 3.6664
Actin 0.0156 0.0185 0.0156 0.0029 0.006 0.0051 0.0209 0.0247 0.0277 0.0047 0.0513 0.012 0.0208 0.0187 0.0053 0.0058 0.0027 0.0034
Bud 0.002 0.0029 0.0044 0.0054 0.0255 0.0016 0.0018 0.0052 0.0031 0.0067 0.0012 0.0006 0.0034 0.0043 0.002 0.0018 0.0003 0.001
Bud Neck 0.0012 0.0009 0.0012 0.0039 0.0438 0.002 0.0024 0.0006 0.0013 0.0004 0.0007 0.0012 0.0045 0.001 0.0009 0.001 0.0002 0.0026
Bud Periphery 0.0049 0.0035 0.0097 0.0044 0.008 0.0025 0.003 0.0071 0.0057 0.01 0.0019 0.0018 0.0068 0.0058 0.0044 0.0068 0.0011 0.0021
Bud Site 0.0091 0.0366 0.0072 0.0073 0.0121 0.001 0.0194 0.018 0.0109 0.0016 0.0027 0.0012 0.0038 0.0377 0.01 0.0036 0.0003 0.0018
Cell Periphery 0.003 0.0008 0.0008 0.0009 0.0013 0.0011 0.0011 0.0008 0.0018 0.0006 0.0003 0.0005 0.0007 0.0012 0.001 0.0011 0.0001 0.0008
Cytoplasm 0.0072 0.0185 0.0068 0.0129 0.0168 0.0105 0.0044 0.0125 0.0177 0.0038 0.0025 0.01 0.0032 0.0092 0.0034 0.0366 0.0054 0.0105
Cytoplasmic Foci 0.0385 0.0519 0.0292 0.019 0.0337 0.0222 0.0295 0.0332 0.0324 0.0064 0.0248 0.022 0.0321 0.0681 0.0308 0.0142 0.0179 0.0357
Eisosomes 0.0013 0.0003 0.0008 0.0002 0.0003 0.0004 0.0005 0.0006 0.0007 0.0001 0.0002 0.0004 0.0005 0.0003 0.0003 0.0004 0.0002 0.0003
Endoplasmic Reticulum 0.0067 0.0018 0.0023 0.0005 0.0004 0.0013 0.0084 0.0014 0.0009 0.0013 0.0008 0.0006 0.0009 0.0008 0.0006 0.0029 0.0002 0.0007
Endosome 0.0652 0.1068 0.0659 0.0726 0.0339 0.0521 0.0796 0.0767 0.0663 0.0226 0.1148 0.0508 0.0735 0.0861 0.0695 0.0402 0.0567 0.0517
Golgi 0.045 0.074 0.0511 0.0241 0.0433 0.0556 0.0886 0.0729 0.0611 0.0073 0.0942 0.0673 0.0508 0.0813 0.0587 0.0237 0.0647 0.0485
Lipid Particles 0.0319 0.0146 0.016 0.0016 0.0144 0.0132 0.0083 0.0047 0.0086 0.0015 0.003 0.0055 0.0353 0.0035 0.0096 0.0015 0.0011 0.0113
Mitochondria 0.6825 0.6148 0.7069 0.8226 0.7175 0.7875 0.6829 0.6827 0.7044 0.8979 0.6807 0.797 0.6795 0.6139 0.7534 0.8342 0.8397 0.7776
None 0.0157 0.0054 0.0149 0.0049 0.0051 0.0125 0.0007 0.0012 0.0005 0.0017 0.0003 0.0028 0.0223 0.0013 0.0021 0.0083 0.0009 0.0058
Nuclear Periphery 0.0022 0.0053 0.002 0.0002 0.0002 0.0006 0.012 0.0003 0.0004 0.0095 0.0004 0.0004 0.0016 0.0005 0.0003 0.0004 0 0.0002
Nucleolus 0.0011 0.0021 0.0006 0.0006 0.0008 0.0005 0.0003 0.0002 0.0014 0.0006 0.0001 0.0002 0.003 0.0006 0.0005 0.0002 0 0.0004
Nucleus 0.0008 0.0021 0.0006 0.0005 0.0019 0.0008 0.0075 0.0004 0.001 0.0033 0.0002 0.0003 0.0023 0.0009 0.0003 0.0007 0.0001 0.0004
Peroxisomes 0.0321 0.0179 0.0513 0.0024 0.0292 0.0174 0.0167 0.0487 0.0304 0.0066 0.007 0.0218 0.0335 0.0456 0.0357 0.0084 0.0058 0.0369
Punctate Nuclear 0.0132 0.0009 0.0006 0.0004 0.0003 0.0005 0.0006 0.0002 0.0003 0.0025 0.0001 0.0002 0.0051 0.0002 0.0002 0.0001 0 0.0004
Vacuole 0.0149 0.0138 0.0068 0.0093 0.0031 0.0055 0.0052 0.0053 0.0171 0.0057 0.0064 0.0017 0.0114 0.0099 0.0058 0.0043 0.001 0.0042
Vacuole Periphery 0.006 0.0064 0.0053 0.0035 0.0026 0.0062 0.0063 0.0027 0.0062 0.0053 0.0066 0.0019 0.0047 0.009 0.0051 0.0037 0.0015 0.0039

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 49.7685 39.0644 36.2343 61.6707 33.8374 60.6597 55.4069 59.2411 63.1996 53.3706
Translational Efficiency 1.0965 1.1715 1.49 1.0395 1.4411 1.2665 1.1695 1.2941 1.5656 1.2925

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✔
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1832 847 147 1976 1805 2330 2363 1808 3637 3177 2510 3784

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 670.06 698.05 944.07 890.31 617.27 820.32 920.32 932.70 643.86 787.72 921.71 910.56
Standard Deviation 90.87 106.93 105.96 187.78 89.23 101.20 125.14 149.03 93.85 116.11 124.23 171.68
Intensity Change Log 2 — 0.059040 0.494604 0.410017 — 0.410285 0.576234 0.595511 — 0.238141 0.534322 0.501941

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000708 0.001471 0.008646 0.008978 0.000352 0.001075 0.004870 0.011534 0.000532 0.001180 0.005092 0.010199
Bud Neck 0.038253 0.022963 0.021789 0.026863 0.034818 0.022166 0.027747 0.016048 0.036548 0.022379 0.027398 0.021696
Bud Site 0.020558 0.047190 0.064151 0.136566* 0.022148 0.020572 0.117926* 0.154271* 0.021347 0.027668 0.114776* 0.145025*
Cell Periphery 0.000248 0.000424 0.000415 0.000303 0.000286 0.000339 0.000246 0.000521 0.000267 0.000362 0.000256 0.000407
Cytoplasm 0.093324 0.043201 0.078057 0.108601* 0.110339* 0.029133 0.075324 0.087329 0.101768* 0.032883 0.075484 0.098437
Cytoplasmic Foci 0.266835* 0.149500 0.145141 0.098348 0.256726* 0.128705 0.080784 0.091128 0.261818* 0.134249 0.084553 0.094898
Eisosomes 0.000276 0.000180 0.000211 0.000105 0.000219 0.000186 0.000084 0.000114 0.000248 0.000184 0.000091 0.000109
Endoplasmic Reticulum 0.001919 0.001378 0.012747 0.002333 0.002107 0.000679 0.002893 0.001471 0.002012 0.000865 0.003470 0.001921
Endosome 0.068255 0.095633 0.082412 0.055132 0.060382 0.084409 0.065301 0.061500 0.064348 0.087401 0.066303 0.058175
Golgi 0.056517 0.098529 0.041150 0.051135 0.052735 0.108445 0.053234 0.087879 0.054640 0.105801 0.052526 0.068691
Lipid Particles 0.026119 0.009491 0.013980 0.002008 0.023611 0.007264 0.001412 0.000895 0.024874 0.007858 0.002148 0.001476
Mitochondria 0.285258* 0.379317* 0.042769 0.167504* 0.290230* 0.489308* 0.219244* 0.248023* 0.287725* 0.459984* 0.208908* 0.205976*
Mitotic Spindle 0.006059 0.021186 0.127694* 0.108789* 0.008567 0.010073 0.124784* 0.078338 0.007303 0.013035 0.124954* 0.094240
None 0.002492 0.003396 0.000758 0.000748 0.005922 0.000665 0.000748 0.000449 0.004194 0.001393 0.000749 0.000606
Nuclear Periphery 0.000851 0.000261 0.002365 0.000812 0.000851 0.000243 0.000828 0.000236 0.000851 0.000248 0.000919 0.000536
Nuclear Periphery Foci 0.000550 0.000628 0.005657 0.000843 0.000517 0.000230 0.000600 0.000245 0.000533 0.000336 0.000896 0.000557
Nucleolus 0.003516 0.003111 0.005159 0.001008 0.004769 0.000988 0.002578 0.000416 0.004138 0.001554 0.002730 0.000725
Nucleus 0.028256 0.012086 0.099622 0.012732 0.031644 0.005473 0.018717 0.004782 0.029937 0.007236 0.023455 0.008933
Peroxisomes 0.041769 0.053299 0.069010 0.048785 0.024402 0.039205 0.028291 0.040363 0.033150 0.042963 0.030676 0.044761
Vacuole 0.044979 0.034494 0.156558 0.144373 0.050891 0.034517 0.145100 0.092031 0.047913 0.034511 0.145771 0.119364
Vacuole Periphery 0.013259 0.022261 0.021710 0.024035 0.018484 0.016325 0.029288 0.022429 0.015852 0.017908 0.028844 0.023268

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -1.79 -4.46 -8.08 -6.79 -0.03 -3.36 -7.63 -7.96 -7.35 -4.32 -3.27 -8.01 -11.26 -10.24 -4.85
Bud Neck 4.94 4.50 3.83 -2.08 -2.05 5.15 2.33 8.03 2.65 7.24 7.33 3.95 7.92 -0.43 3.85
Bud Site -6.57 -4.40 -25.92 -15.14 -7.19 0.75 -24.93* -26.64* -27.29* -6.15 -3.72 -26.43 -37.10 -33.66* -6.20
Cell Periphery -3.71 -2.45 -1.22 2.47 1.75 -1.08 1.07 -1.74 -1.28 -2.10 -2.65 0.49 -2.00 -0.44 -2.09
Cytoplasm 9.84 0.80 -4.15 -13.67 -2.67 18.18 5.66 3.40 -16.12 -2.28 21.79 5.07 -0.61 -23.28 -5.76
Cytoplasmic Foci 15.68 11.61 29.93 8.83 4.13 21.11 31.77 28.61 8.76 -2.95 27.97 42.48 41.35 11.84 -3.37
Eisosomes 3.28 1.57 6.51 5.22 5.55 1.45 7.20 5.48 4.88 -4.20 3.31 9.54 8.50 6.68 -3.33
Endoplasmic Reticulum 1.22 -5.94 -2.23 -2.80 5.61 5.23 -2.96 1.97 -3.88 5.28 5.31 -5.66 -0.39 -6.13 5.57
Endosome -5.30 -0.91 5.02 8.89 2.94 -6.86 -0.94 0.07 7.06 1.04 -8.07 0.16 3.71 11.61 3.33
Golgi -8.06 2.84 3.03 9.96 -1.54 -14.11 0.72 -7.79 5.19 -8.53 -16.42 1.91 -3.95 12.30 -5.28
Lipid Particles 7.57 3.73 14.42 4.99 4.99 9.55 13.91 14.33 9.16 3.46 13.09 19.43 20.30 9.72 3.03
Mitochondria -6.89 22.98 14.85 17.70 -12.32 -20.11* 8.90 5.04 25.95 -3.55 -22.10* 12.12 13.88 36.12* 0.51
Mitotic Spindle -5.78 -6.60 -22.48 -16.83 0.79 -1.06 -26.44* -18.37 -18.42 8.04 -5.11 -28.16 -28.89 -26.42 5.90
None -0.84 3.31 3.30 2.45 0.17 5.55 5.49 5.83 1.73 4.43 4.51 6.44 6.68 2.54 1.47
Nuclear Periphery 3.48 -3.62 -0.27 -3.36 3.39 2.26 -0.27 2.22 -0.35 6.62 3.71 -0.92 1.32 -3.09 3.01
Nuclear Periphery Foci -0.25 -3.97 -1.47 -0.88 3.74 2.34 -1.16 2.11 -0.53 4.12 1.58 -3.24 -0.49 -2.05 2.75
Nucleolus 0.19 -1.39 3.85 2.38 3.38 5.22 2.07 6.06 2.65 6.42 4.84 1.47 7.08 2.65 5.99
Nucleus 6.01 -5.75 4.89 -1.99 6.52 12.07 3.49 12.01 -0.37 10.20 14.61 0.84 11.51 -3.93 8.59
Peroxisomes -2.76 -2.17 -1.66 1.43 1.61 -5.95 -1.61 -5.89 -0.30 -4.48 -4.45 1.55 -4.95 -0.29 -5.94
Vacuole 3.15 -8.66 -23.13 -24.24 0.57 5.80 -22.78 -11.56 -16.34 9.39 6.44 -26.28 -25.00 -29.53 4.15
Vacuole Periphery -5.09 -1.12 -5.63 0.04 -0.63 1.74 -6.60 -2.11 -3.75 3.84 -2.35 -9.44 -5.50 -3.45 3.90
Standard name
Human Ortholog
Description Mitochondrial NADH-cytochrome b5 reductase; involved in ergosterol biosynthesis
Localization
Cell Percentages mitochondrion (74%)
Cell Cycle Regulation No
Subcompartmental Group mito-1

Mcr1

Mcr1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Mcr1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available