Standard name
Human Ortholog
Description Phosphatase with sequence similarity to Cdc25p; Arr2p and Mih1p; member of the single-domain rhodanese homology superfamily; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.98 0.98 0.97 0.95 0.93 0.86 – 0.74 0.76 0.73 0.7 0.73 0.97 0.98 0.98 0.95 0.97 0.96 0.94 0.97 0.89 0.9 0.9 0.9
Endoplasmic Reticulum 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0.06 0 0.16 – 0.12 0.23 0.19 0.28 0.25 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 – 0.05 0 0.06 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0.07 0 – 0.17 0.1 0.08 0.11 0.08 0 0 0 0.07 0 0 0 0 0.05 0.05 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 1 0 0 0 – 0 0 0 0 0 1 0 0 0 0 0 1 0 0 0 0 4
Bud 0 0 2 2 4 2 – 5 5 2 4 12 1 0 1 0 0 0 3 0 0 1 4 7
Bud Neck 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1
Bud Site 0 0 0 0 0 0 – 0 0 1 1 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 1 1 0 0 0 0 – 4 5 7 4 7 0 5 5 6 6 7 0 0 0 0 0 0
Cytoplasm 313 111 98 195 350 310 – 487 254 339 187 306 394 570 772 211 239 217 312 114 99 123 473 550
Endoplasmic Reticulum 1 0 1 1 1 3 – 2 6 12 2 3 5 5 2 5 7 4 1 0 0 0 4 4
Endosome 0 0 0 0 0 0 – 1 0 0 0 1 0 0 1 2 0 1 1 0 0 0 3 4
Golgi 0 0 0 0 0 0 – 0 0 0 0 0 0 0 2 0 0 0 1 0 0 0 2 6
Mitochondria 2 2 4 13 11 56 – 78 76 90 76 105 4 0 2 3 6 2 1 0 2 1 3 1
Nucleus 2 2 0 2 3 2 – 30 6 30 10 13 13 9 12 5 2 4 1 0 0 1 5 3
Nuclear Periphery 0 0 0 1 0 1 – 3 0 3 2 2 0 1 0 0 0 0 0 0 0 0 2 1
Nucleolus 0 0 0 0 0 1 – 0 0 1 1 1 0 0 0 0 1 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 1 0 0 – 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 6 0 0 5 26 16 – 112 35 35 29 33 0 14 10 16 8 9 4 0 5 6 22 18
Unique Cell Count 321 113 101 206 377 361 662 336 463 269 417 408 583 791 223 246 225 331 118 111 137 527 610
Labelled Cell Count 325 116 106 220 395 391 723 387 520 316 483 418 604 807 248 269 244 331 118 111 137 527 610


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 8.4 9.1 6.2 5.8 6.4 5.8 – 6.2 5.8 6.2 6.1 6.4 9.3 9.4 9.1 9.3 9.2 9.3 8.0 8.7 8.7
Std Deviation (1e-4) 1.2 1.9 0.9 1.1 1.2 1.3 – 1.2 1.4 1.3 1.3 1.7 1.4 5.1 1.5 2.1 2.1 2.4 1.9 1.8 1.7
Intensity Change (Log2) – – – -0.07 0.05 -0.07 – 0.0 -0.08 0.02 -0.0 0.05 0.59 0.61 0.56 0.6 0.58 0.6 0.38 0.5 0.5


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm -0.9 -1.5 -3.1 – -5.2 -4.8 -5.2 -5.6 -5.1 -0.2 0.5 0.3 -1.0 0.1 -0.3
Endoplasmic Reticulum 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.8 0 3.1 – 2.4 4.3 3.8 5.1 4.7 0 0 0 0 0 0
Nucleus 0 0 0 – 0 0 2.6 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 2.7 0 – 4.5 3.4 2.9 3.4 2.9 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 5.6938 8.0268 7.151 6.2505 6.0936 6.9723 3.9046 6.1355 5.5762 4.4875 3.9805 5.5706 7.7807 10.0135 8.8836 8.528 7.5776 8.5569
Actin 0.0191 0.0021 0.0027 0.0067 0.0474 0.0055 0.0227 0.0005 0.0025 0.0681 0.0338 0.0043 0.0186 0.0003 0.001 0.0008 0.0109 0.0009
Bud 0.0072 0.0026 0.0034 0.0169 0.0126 0.0017 0.0013 0.0001 0.0007 0.0003 0.0004 0.001 0.0004 0.0001 0.0019 0.0001 0.0002 0.0002
Bud Neck 0.0026 0.0004 0.0006 0.001 0.0011 0.002 0.009 0.0001 0.0032 0.0012 0.0005 0.0008 0.0018 0.0002 0.0004 0.0008 0.0025 0.0012
Bud Periphery 0.0041 0.0005 0.0006 0.0054 0.0033 0.0007 0.0016 0 0.0008 0.0005 0.0004 0.001 0.0002 0 0.0004 0 0.0003 0
Bud Site 0.0033 0.004 0.0034 0.0013 0.0028 0.0003 0.015 0.0003 0.0042 0.0005 0.0031 0.0001 0.0031 0.0002 0.0009 0.0001 0.0008 0
Cell Periphery 0.0003 0.0001 0.0001 0.0001 0.0001 0 0.0004 0 0.0001 0.0001 0.0001 0 0.0001 0 0 0 0.0002 0
Cytoplasm 0.7796 0.9631 0.9263 0.8671 0.7522 0.9129 0.8089 0.985 0.9477 0.8454 0.9029 0.9504 0.9211 0.9835 0.9494 0.9548 0.8909 0.9463
Cytoplasmic Foci 0.0228 0.0037 0.0083 0.0097 0.0204 0.0099 0.0228 0.0022 0.0051 0.0088 0.014 0.0037 0.0063 0.0019 0.0136 0.003 0.0111 0.0025
Eisosomes 0.0002 0 0 0 0.0001 0 0.0002 0 0 0.0005 0.0001 0 0.0001 0 0 0 0.0004 0
Endoplasmic Reticulum 0.0082 0.0017 0.0022 0.0052 0.0024 0.0027 0.0045 0.001 0.0012 0.0023 0.0015 0.0026 0.0023 0.0006 0.0011 0.0025 0.0019 0.0014
Endosome 0.0423 0.0018 0.0096 0.0056 0.0366 0.0072 0.0264 0.0005 0.0018 0.0085 0.0072 0.0043 0.0102 0.0004 0.0042 0.0031 0.008 0.0011
Golgi 0.0073 0.0002 0.0012 0.0027 0.0041 0.002 0.0057 0.0001 0.0013 0.0086 0.0062 0.0011 0.0014 0 0.0016 0.0001 0.0038 0.0001
Lipid Particles 0.0114 0.0001 0.0017 0.0013 0.0023 0.0016 0.0047 0 0.0028 0.0173 0.0062 0.0016 0.0022 0 0.0006 0.0001 0.0044 0
Mitochondria 0.0133 0.0003 0.0006 0.0013 0.0518 0.0015 0.0134 0.0001 0.0007 0.0036 0.001 0.0005 0.001 0.0001 0.0005 0.0002 0.0043 0.0001
None 0.0126 0.0037 0.0074 0.0068 0.0075 0.0054 0.0234 0.0062 0.0092 0.0091 0.0033 0.0029 0.009 0.0023 0.0029 0.0044 0.003 0.002
Nuclear Periphery 0.0143 0.0017 0.0047 0.0051 0.0041 0.0051 0.0073 0.0006 0.0013 0.0027 0.0013 0.0039 0.0024 0.0007 0.0017 0.006 0.0037 0.0024
Nucleolus 0.0009 0.0001 0.0001 0.0001 0.0005 0.0002 0.0008 0 0.0001 0.0002 0.0001 0 0.0002 0 0.0002 0.0001 0.0007 0
Nucleus 0.0218 0.0109 0.0194 0.0389 0.0374 0.0325 0.01 0.0029 0.0106 0.007 0.0064 0.0193 0.0131 0.0088 0.0157 0.0179 0.0224 0.0402
Peroxisomes 0.0034 0.0004 0.0018 0.0194 0.0033 0.0013 0.0151 0.0001 0.0052 0.0117 0.008 0.0003 0.0024 0 0.0009 0.0001 0.0257 0.0001
Punctate Nuclear 0.0164 0.0019 0.0043 0.0033 0.0061 0.0061 0.003 0.0003 0.0009 0.0027 0.003 0.0011 0.0033 0.0006 0.0027 0.0051 0.0026 0.001
Vacuole 0.0064 0.0008 0.0012 0.0018 0.0031 0.001 0.0028 0.0002 0.0007 0.0005 0.0003 0.0005 0.0008 0.0002 0.0003 0.0004 0.0012 0.0004
Vacuole Periphery 0.0025 0.0001 0.0005 0.0003 0.0008 0.0004 0.001 0 0.0001 0.0005 0.0001 0.0003 0.0002 0 0.0001 0.0001 0.0007 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 32.0901 50.7531 62.2226 29.0519 17.6026 39.4736 45.1808 39.1528 40.4818 30.7092
Translational Efficiency 1.1501 1.1722 0.9744 2.5678 3.3285 1.1656 1.1805 2.0597 1.3669 1.4286

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1530 1249 122 543 2348 2161 1701 1580 3878 3410 1823 2123

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 761.68 867.17 1412.59 1046.06 868.62 924.15 1083.00 1113.23 826.43 903.28 1105.06 1096.05
Standard Deviation 111.75 154.19 194.46 171.62 224.17 126.66 167.53 147.70 195.15 140.10 188.42 156.93
Intensity Change Log 2 — 0.187130 0.891086 0.457709 — 0.089402 0.318236 0.357955 — 0.135886 0.614243 0.405420

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000088 0.000286 0.002038 0.003317 0.000174 0.000146 0.000782 0.001613 0.000140 0.000197 0.000866 0.002049
Bud Neck 0.001497 0.002013 0.004058 0.013438 0.002644 0.003412 0.002093 0.009083 0.002191 0.002900 0.002224 0.010197
Bud Site 0.002536 0.007106 0.008808 0.045252 0.004154 0.004498 0.008012 0.038491 0.003516 0.005453 0.008065 0.040221
Cell Periphery 0.000084 0.000112 0.000449 0.000291 0.000060 0.000039 0.000070 0.000110 0.000070 0.000066 0.000095 0.000157
Cytoplasm 0.757027* 0.779984* 0.493877* 0.455803* 0.758748* 0.767613* 0.646343* 0.645947* 0.758069* 0.772144* 0.636140* 0.597314*
Cytoplasmic Foci 0.061696 0.045516 0.020846 0.040415 0.048850 0.058499 0.003369 0.013613 0.053918 0.053743 0.004539 0.020468
Eisosomes 0.000021 0.000016 0.000037 0.000058 0.000016 0.000017 0.000008 0.000020 0.000018 0.000017 0.000010 0.000029
Endoplasmic Reticulum 0.000753 0.001084 0.014285 0.002671 0.000673 0.000486 0.000840 0.000626 0.000704 0.000705 0.001740 0.001149
Endosome 0.002384 0.003596 0.007328 0.040278 0.001539 0.003439 0.000463 0.010922 0.001873 0.003497 0.000923 0.018431
Golgi 0.000432 0.001004 0.002749 0.016330 0.000568 0.002198 0.000089 0.007655 0.000514 0.001761 0.000267 0.009874
Lipid Particles 0.001041 0.000868 0.002457 0.002543 0.000703 0.000907 0.000349 0.000494 0.000837 0.000893 0.000490 0.001018
Mitochondria 0.000631 0.001405 0.000513 0.010057 0.001885 0.002368 0.000300 0.007047 0.001390 0.002015 0.000314 0.007817
Mitotic Spindle 0.000173 0.007178 0.003825 0.076300 0.003093 0.005300 0.005041 0.055769 0.001941 0.005988 0.004960 0.061020
None 0.014376 0.010022 0.003850 0.010572 0.004765 0.003785 0.010256 0.005949 0.008557 0.006070 0.009827 0.007132
Nuclear Periphery 0.000171 0.000334 0.001288 0.001070 0.000203 0.000146 0.000425 0.000364 0.000190 0.000215 0.000483 0.000545
Nuclear Periphery Foci 0.001223 0.001812 0.011522 0.002120 0.000685 0.000546 0.001362 0.001259 0.000898 0.001010 0.002042 0.001479
Nucleolus 0.000504 0.000430 0.000251 0.001840 0.000311 0.000379 0.000117 0.000270 0.000387 0.000397 0.000126 0.000672
Nucleus 0.125465 0.095288 0.313233* 0.072947 0.123405 0.090200 0.289322* 0.114263 0.124217 0.092064 0.290922* 0.103695
Peroxisomes 0.000608 0.001538 0.000304 0.005709 0.000369 0.001935 0.000407 0.001972 0.000463 0.001789 0.000400 0.002928
Vacuole 0.028948 0.039269 0.107526 0.183649 0.046160 0.053668 0.030289 0.080335 0.039369 0.048394 0.035458 0.106760
Vacuole Periphery 0.000341 0.001141 0.000757 0.015340 0.000993 0.000418 0.000061 0.004196 0.000736 0.000683 0.000108 0.007047

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -2.40 -5.90 -2.81 -2.02 2.64 0.54 -1.88 -2.42 -2.71 0.13 -1.31 -2.72 -3.70 -3.22 0.18
Bud Neck -1.31 -6.78 -2.73 -2.14 1.65 -1.84 1.30 -1.46 -0.38 -2.17 -2.37 -0.59 -2.76 -1.51 -2.30
Bud Site -4.26 -7.98 -5.03 -2.89 -1.57 -0.39 -1.23 -4.85 -4.85 -3.60 -3.05 -2.35 -6.84 -5.37 -4.30
Cell Periphery -0.82 -10.30 -1.92 -0.12 9.24 3.86 -4.11 -1.34 -6.00 2.77 0.49 -7.22 -1.72 -1.38 4.87
Cytoplasm -1.86 16.26 24.52 24.77 -1.09 -1.19 27.13 23.86 24.24 -0.91 -2.04 31.76 32.88 33.30 2.15
Cytoplasmic Foci 4.70 10.96 17.04 12.06 2.22 -3.50 26.44 22.87 22.09 -7.28 -0.04 34.07 29.34 25.73 -7.31
Eisosomes 2.81 -5.57 -4.56 -6.27 2.80 -1.21 3.68 -2.57 -1.40 -6.60 0.99 3.81 -4.07 -4.93 -7.38
Endoplasmic Reticulum -1.37 -2.20 -1.77 -0.02 2.12 1.15 -2.85 1.68 -0.48 5.31 0.16 -3.00 0.36 0.02 3.12
Endosome -2.42 -2.53 -2.72 -2.05 -0.48 -5.82 9.97 -1.06 2.00 -2.87 -5.94 6.60 -2.47 0.11 -4.10
Golgi -1.85 -1.24 -1.99 -1.49 -0.85 -3.06 2.46 -1.44 1.27 -2.54 -3.50 2.49 -2.42 0.12 -3.19
Lipid Particles 0.76 -5.96 -1.87 -2.60 4.75 -0.66 0.23 -0.55 0.40 -3.40 -0.21 0.12 -1.01 -0.56 -2.52
Mitochondria -0.97 0.13 -1.76 -0.67 -2.17 -0.50 2.50 0.46 0.96 -2.27 -0.92 2.51 -0.41 0.55 -3.03
Mitotic Spindle -4.14 -4.00 -5.92 -4.16 -4.97 -1.56 -3.21 -5.49 -4.45 -3.62 -3.80 -4.65 -8.06 -5.90 -5.26
None 2.49 7.09 3.67 1.39 -2.97 1.17 -5.97 -3.06 -6.94 5.19 2.94 -0.61 1.77 -1.82 3.84
Nuclear Periphery -1.06 -11.61 -11.12 -5.07 4.17 2.43 -18.39 -18.15 -24.61 -0.29 -0.39 -23.08 -21.60 -11.86 -0.55
Nuclear Periphery Foci -3.49 -6.94 -2.19 1.85 6.71 1.77 -5.31 -5.44 -7.15 1.64 -1.51 -6.96 -4.88 -2.80 4.58
Nucleolus 0.43 2.44 0.21 -0.10 -1.38 -1.21 2.83 2.09 3.35 -1.60 -0.51 4.40 1.99 2.55 -2.50
Nucleus 5.80 -10.84 4.25 -0.30 12.00 9.26 -35.00 -9.27 -17.24 25.13 10.83 -37.55 -6.49 -15.11 30.24
Peroxisomes -1.30 0.96 -1.00 0.74 -2.55 -4.97 1.73 -0.61 4.21 -1.49 -4.07 1.80 -0.78 3.58 -2.53
Vacuole -5.33 -8.82 -26.73 -25.03 -9.25 -2.63 -6.47 -24.41 -22.82 -18.70 -5.35 -11.09 -34.74 -32.18 -24.73
Vacuole Periphery -1.68 -0.88 -2.14 -1.32 -1.95 1.45 2.40 0.52 -1.16 -2.52 0.26 2.74 -1.16 -1.47 -3.36
Standard name
Human Ortholog
Description Phosphatase with sequence similarity to Cdc25p; Arr2p and Mih1p; member of the single-domain rhodanese homology superfamily; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus
Localization
Cell Percentages cytoplasm (99%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

Ych1

Ych1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Ych1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available