Standard name
Human Ortholog
Description Mitochondrial inner membrane protein; binds to the 5' UTR of the COX3 mRNA to activate its translation together with Pet122p and Pet494p; also binds to the COX1 Group I intron AI5 beta to facilitate exon ligation during splicing
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0.05 0 0 0 0 0 0 0 0 0 0.05 0.06 0 – 0 – 0 0 0 0 0 0
Bud 0 0 0.07 0 0 0 0 0 0 0.05 0 0.07 0 0 0 – 0 – 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 – 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 – – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 – 0 – 0 0 0 0 0 0
Cytoplasm 0.05 0.15 0.16 0.15 0.15 0.11 0.08 0.13 0.05 0.05 0.05 0 0.17 0.39 0.62 – 0.26 – 0 0 0 0 0 0
Endoplasmic Reticulum 0.05 0 0 0 0 0 0 0 0 0 0 0 0.08 0.06 0 – 0 – 0 0 0 0 0 0
Endosome 0.06 0.06 0.11 0.11 0.24 0.1 0.1 0.09 0 0 0 0 0.1 0.15 0.11 – 0.26 – 0.1 0.08 0.09 0.05 0.05 0.09
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 – 0 0 0 0 0 0
Mitochondria 0.76 0.78 0.32 0.61 0.42 0.7 0.69 0.61 0.91 0.87 0.92 0.91 0.56 0.21 0.07 – 0.15 – 0.72 0.73 0.71 0.64 0.71 0.72
Nucleus 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 – 0.06 – 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 – 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 – 0 0 0 0 0 0
Peroxisomes 0.05 0.06 0.14 0.17 0.11 0 0.05 0 0 0 0 0 0 0 0.09 – 0.24 – 0.05 0 0.06 0.08 0 0
SpindlePole 0.08 0 0.27 0.1 0.16 0.09 0.14 0.15 0.07 0.09 0.06 0.1 0.08 0.09 0.06 – 0.15 – 0 0 0 0 0 0
Vac/Vac Membrane 0.12 0.12 0.07 0.06 0.09 0.08 0.11 0.13 0 0.06 0 0.05 0.08 0.06 0.05 – 0.18 – 0 0 0 0.08 0.07 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 2 5 6 2 6 1 0 3 1 0 2 0 4 6 0 – 0 – 0 3 5 0 5 3
Bud 1 6 8 4 5 8 11 15 16 23 16 31 3 4 0 – 1 – 0 2 0 0 0 1
Bud Neck 0 0 3 0 1 1 2 3 1 1 0 0 1 1 2 – 0 – 1 1 0 0 1 1
Bud Site 0 0 0 0 1 2 2 2 10 1 10 9 0 0 0 – 0 – – – – – – –
Cell Periphery 2 7 4 4 5 6 2 4 1 3 2 2 4 1 2 – 1 – 0 0 0 0 0 0
Cytoplasm 7 30 18 22 32 32 29 44 19 25 17 19 15 37 87 – 9 – 1 7 0 1 3 3
Endoplasmic Reticulum 7 4 0 0 8 5 1 0 4 2 1 1 7 6 3 – 1 – 2 1 0 0 3 2
Endosome 9 12 12 16 53 30 37 30 1 11 3 12 9 14 16 – 9 – 16 18 17 2 8 18
Golgi 0 2 1 0 0 0 0 0 0 0 0 0 0 0 1 – 0 – 4 7 4 0 4 1
Mitochondria 107 153 36 88 92 212 264 206 357 416 330 413 49 20 10 – 5 – 124 173 134 33 121 149
Nucleus 0 1 0 0 2 16 13 14 10 9 4 9 0 1 0 – 2 – 0 2 0 0 0 1
Nuclear Periphery 0 1 0 0 0 1 1 0 4 2 4 2 0 0 0 – 0 – 1 0 0 0 0 1
Nucleolus 1 0 0 0 0 1 5 2 3 3 3 4 0 0 0 – 0 – 1 1 1 0 0 1
Peroxisomes 7 12 16 25 23 9 19 15 5 8 4 10 2 2 13 – 8 – 9 5 12 3 3 7
SpindlePole 11 4 30 15 36 27 52 49 26 44 21 45 7 9 8 – 5 – 3 1 3 1 2 4
Vac/Vac Membrane 17 23 8 8 20 25 43 42 11 27 12 24 7 6 7 – 6 – 3 8 2 4 11 9
Unique Cell Count 141 197 112 144 219 304 383 335 392 476 360 456 87 95 140 34 173 239 191 52 172 209
Labelled Cell Count 171 260 142 184 284 376 481 429 469 575 429 581 108 107 149 47 173 239 191 52 172 209


Mitochondria

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.5 4.8 5.4 5.1 5.5 4.6 4.7 5.1 3.8 4.3 4.0 4.2 4.7 5.2 5.1 – 5.5 – – 5.8 5.8
Std Deviation (1e-4) 0.8 1.6 1.4 1.4 0.9 1.2 1.4 1.9 1.0 1.1 0.9 1.7 0.8 1.1 1.1 – 1.9 – – 2.4 1.3
Intensity Change (Log2) – – – -0.1 0.03 -0.25 -0.21 -0.09 -0.5 -0.33 -0.44 -0.36 -0.2 -0.05 -0.09 – 0.04 – – 0.09 0.09


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 1.268 1.5989 1.3105 1.0927 0.4333 1.1105 2.1647 2.0962 2.0621 1.8253 1.3512 1.9449 -0.7667 -0.0405 -0.4532 -0.3037 -0.6397 -0.5032
Actin 0.0548 0.0125 0.0381 0.0381 0.0397 0.019 0.0432 0.0187 0.0439 0.0188 0.0035 0.0314 0.033 0.0216 0.0432 0.0099 0.0264 0.0165
Bud 0.001 0.0035 0.0034 0.0022 0.0021 0.0011 0.0013 0.0026 0.0034 0.0078 0.001 0.0019 0.001 0.0017 0.0021 0.0059 0.0075 0.0007
Bud Neck 0.0039 0.0018 0.0057 0.0365 0.0024 0.0129 0.01 0.0007 0.0041 0.046 0.0016 0.0107 0.0018 0.0019 0.0087 0.0025 0.0019 0.0015
Bud Periphery 0.0026 0.007 0.0107 0.0062 0.0067 0.0047 0.003 0.006 0.0128 0.04 0.0025 0.0109 0.0017 0.0022 0.0035 0.0139 0.0187 0.0015
Bud Site 0.0166 0.0494 0.0256 0.0114 0.0027 0.0017 0.0169 0.0096 0.0172 0.0113 0.0043 0.0027 0.0039 0.0433 0.0145 0.0094 0.0068 0.0009
Cell Periphery 0.0047 0.0018 0.0069 0.0034 0.0018 0.0027 0.0055 0.0024 0.0052 0.0323 0.0008 0.0173 0.001 0.0014 0.0009 0.0013 0.0006 0.0005
Cytoplasm 0.0195 0.0274 0.0184 0.0119 0.0053 0.0269 0.0396 0.0259 0.0266 0.0357 0.078 0.0193 0.0301 0.0393 0.0282 0.0231 0.012 0.0186
Cytoplasmic Foci 0.0887 0.1337 0.0726 0.1304 0.0312 0.082 0.0605 0.0969 0.055 0.0881 0.0588 0.0812 0.0955 0.113 0.0792 0.1103 0.0951 0.0807
Eisosomes 0.0032 0.0008 0.0023 0.0051 0.0011 0.0015 0.0104 0.0024 0.0029 0.0028 0.0003 0.0018 0.0008 0.0005 0.0012 0.0003 0.0003 0.0003
Endoplasmic Reticulum 0.0058 0.0015 0.003 0.0009 0.0046 0.0031 0.0127 0.0016 0.0034 0.0016 0.0012 0.0028 0.0096 0.0025 0.0072 0.0055 0.0039 0.0029
Endosome 0.1172 0.1144 0.0701 0.0927 0.0528 0.06 0.0897 0.1299 0.109 0.1038 0.1042 0.0699 0.1861 0.1924 0.1127 0.123 0.2091 0.1671
Golgi 0.0456 0.0715 0.0469 0.0518 0.0487 0.0523 0.0658 0.0859 0.066 0.1061 0.0729 0.0709 0.1146 0.1335 0.0755 0.0788 0.0867 0.1233
Lipid Particles 0.1086 0.0262 0.0205 0.01 0.0174 0.0398 0.0651 0.0446 0.0299 0.075 0.0076 0.0366 0.0854 0.0304 0.0242 0.0467 0.0147 0.0318
Mitochondria 0.288 0.4241 0.5198 0.5032 0.7272 0.5572 0.3716 0.3689 0.4629 0.2891 0.6199 0.5004 0.314 0.3069 0.5115 0.4541 0.4319 0.4564
None 0.0839 0.0129 0.0521 0.0469 0.0062 0.0516 0.0869 0.0727 0.088 0.0469 0.0078 0.052 0.0442 0.0409 0.0156 0.0104 0.0073 0.025
Nuclear Periphery 0.0118 0.0004 0.0009 0.0002 0.0057 0.0003 0.0104 0.0003 0.0011 0.0005 0.0002 0.0005 0.0141 0.001 0.0009 0.0009 0.0079 0.0025
Nucleolus 0.0027 0.0015 0.0015 0.0007 0.0009 0.0011 0.0058 0.0007 0.0011 0.0024 0.0003 0.0004 0.0014 0.0007 0.0005 0.0015 0.0012 0.0003
Nucleus 0.004 0.0014 0.0013 0.0005 0.0017 0.0008 0.0225 0.0005 0.0012 0.0012 0.0005 0.0005 0.0031 0.0009 0.0008 0.001 0.0019 0.0006
Peroxisomes 0.0913 0.0926 0.0865 0.042 0.0336 0.0738 0.0521 0.1196 0.0494 0.0743 0.0281 0.0804 0.038 0.0436 0.0524 0.0833 0.0315 0.0461
Punctate Nuclear 0.0082 0.0012 0.0008 0.0007 0.002 0.0012 0.009 0.0005 0.0007 0.0011 0.0003 0.0005 0.0019 0.0006 0.0056 0.003 0.0185 0.0004
Vacuole 0.0279 0.0109 0.0083 0.0034 0.0034 0.0038 0.0116 0.0076 0.0116 0.0107 0.0036 0.0044 0.0121 0.0147 0.0075 0.0084 0.0084 0.0102
Vacuole Periphery 0.01 0.0035 0.0046 0.002 0.0028 0.0024 0.0063 0.0022 0.0045 0.0046 0.0025 0.0037 0.0065 0.007 0.0038 0.0068 0.0076 0.0121

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 15.6378 11.1834 13.2777 16.1285 15.4091 5.0013 10.7422 10.303 15.935 20.6183
Translational Efficiency 0.4047 0.4249 0.5555 0.5629 0.3591 1.6775 0.8374 1.1962 0.5274 0.393

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1703 1282 2604 821 1393 1163 157 1481 3096 2445 2761 2302

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 672.91 780.46 830.55 861.41 603.46 837.60 956.95 851.32 641.66 807.64 837.74 854.92
Standard Deviation 75.00 101.61 95.39 113.73 80.84 102.23 101.05 124.92 85.02 105.83 100.09 121.14
Intensity Change Log 2 — 0.213911 0.303653 0.356287 — 0.473003 0.665185 0.496443 — 0.342219 0.485897 0.424251

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.001172 0.003046 0.005351 0.021653 0.000487 0.004542 0.010701 0.013926 0.000864 0.003758 0.005656 0.016682
Bud Neck 0.012601 0.010449 0.028952 0.015741 0.019317 0.016817 0.021053 0.012759 0.015623 0.013478 0.028503 0.013823
Bud Site 0.011515 0.028138 0.045070 0.100195* 0.011375 0.023520 0.050890 0.097866 0.011452 0.025942 0.045401 0.098697
Cell Periphery 0.000250 0.000438 0.000192 0.000418 0.000393 0.000479 0.000374 0.000422 0.000315 0.000457 0.000202 0.000421
Cytoplasm 0.015581 0.012971 0.035763 0.044075 0.021568 0.013289 0.031416 0.039854 0.018275 0.013122 0.035516 0.041360
Cytoplasmic Foci 0.108934 0.110629 0.116683 0.150202 0.110930 0.100244 0.190096* 0.118047 0.109832 0.105689 0.120857 0.129515
Eisosomes 0.000171 0.000179 0.000125 0.000221 0.000138 0.000180 0.000218 0.000170 0.000156 0.000180 0.000131 0.000188
Endoplasmic Reticulum 0.001011 0.000526 0.003668 0.003040 0.000960 0.000432 0.007567 0.001355 0.000988 0.000481 0.003890 0.001956
Endosome 0.080094 0.099965 0.101718 0.089237 0.101253 0.087231 0.113393 0.089519 0.089614 0.093908 0.102382 0.089418
Golgi 0.139708* 0.177324* 0.058401 0.089409 0.107447 0.141776* 0.075254 0.116176* 0.125193* 0.160415* 0.059359 0.106630
Lipid Particles 0.011125 0.004621 0.011874 0.003135 0.019768 0.003411 0.014863 0.001315 0.015014 0.004045 0.012044 0.001964
Mitochondria 0.526612* 0.429024* 0.256910* 0.144741* 0.477594* 0.463139* 0.075746 0.258238* 0.504557* 0.445251* 0.246609* 0.217759*
Mitotic Spindle 0.005586 0.013060 0.100371* 0.089216 0.008960 0.019835 0.061423 0.058817 0.007104 0.016283 0.098156 0.069659
None 0.000794 0.000294 0.000930 0.000221 0.002778 0.000347 0.002235 0.000275 0.001687 0.000319 0.001005 0.000256
Nuclear Periphery 0.000279 0.000050 0.003608 0.000400 0.000574 0.000052 0.001472 0.000209 0.000412 0.000051 0.003487 0.000277
Nuclear Periphery Foci 0.000302 0.000196 0.002168 0.000692 0.000563 0.000113 0.005549 0.000277 0.000419 0.000157 0.002361 0.000425
Nucleolus 0.001125 0.000406 0.004992 0.001139 0.004867 0.000410 0.004260 0.000316 0.002809 0.000408 0.004951 0.000610
Nucleus 0.003488 0.001486 0.023066 0.005873 0.009724 0.003209 0.047951 0.002275 0.006294 0.002305 0.024481 0.003558
Peroxisomes 0.049928 0.079947 0.058762 0.161724* 0.054078 0.093718 0.176808* 0.117019* 0.051795 0.086497 0.065474 0.132963*
Vacuole 0.016179 0.016076 0.120891 0.067576 0.024422 0.019458 0.095645 0.056684 0.019888 0.017685 0.119455 0.060569
Vacuole Periphery 0.013544 0.011175 0.020504 0.011092 0.022803 0.007798 0.013089 0.014479 0.017710 0.009569 0.020083 0.013271

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -4.61 -6.74 -8.95 -8.02 -6.86 -5.50 -2.48 -9.63 -5.90 -0.84 -7.03 -7.78 -12.99 -10.05 -8.08
Bud Neck 1.25 -8.59 -1.82 -3.30 7.06 0.88 -0.63 3.00 1.52 2.11 1.33 -7.73 1.07 -0.46 9.25
Bud Site -6.63 -15.62 -15.82 -11.99 -9.35 -5.24 -5.50 -20.66 -16.15 -5.65 -8.44 -17.25 -26.02 -19.95 -14.10
Cell Periphery -3.80 2.49 -2.49 0.47 -3.61 -0.66 0.28 -0.48 0.44 -0.65 -2.14 4.89 -2.50 0.59 -5.84
Cytoplasm 1.28 -9.87 -9.04 -9.57 -3.00 3.47 -2.16 -6.61 -10.35 -1.44 3.37 -9.21 -11.57 -14.16 -2.73
Cytoplasmic Foci -0.30 -1.70 -6.56 -6.16 -5.63 2.13 -5.77 -1.16 -3.48 5.34 1.28 -2.75 -4.84 -6.01 -2.18
Eisosomes -0.38 3.77 -2.62 -1.55 -5.45 -2.76 -4.54 -2.90 0.68 2.99 -1.72 2.65 -3.12 -0.48 -5.29
Endoplasmic Reticulum 2.20 -8.32 -2.81 -3.58 0.86 3.50 -4.70 -2.11 -4.91 4.38 3.62 -10.52 -3.34 -5.33 5.22
Endosome -4.02 -4.75 -1.13 2.26 2.49 2.46 -0.75 2.40 -0.26 2.14 -1.16 -2.92 0.50 1.58 3.27
Golgi -5.22 16.38 8.16 12.48 -5.36 -5.01 3.77 -1.10 4.13 -4.44 -7.01 17.73 4.76 11.32 -12.21
Lipid Particles 4.98 -0.54 6.33 1.44 7.63 8.71 1.54 10.15 3.78 5.85 9.87 2.01 12.39 3.99 10.24
Mitochondria 7.52 27.57 33.15* 22.74 11.29 0.96 23.81 17.98 16.24 -11.34 6.18 32.04 33.50 25.10 3.35
Mitotic Spindle -5.29 -25.00* -12.66 -11.40 1.61 -5.14 -4.49 -13.75 -9.72 0.24 -7.33 -25.07 -18.87 -15.44 6.03
None 2.56 -0.24 2.07 -0.69 2.72 4.52 0.46 4.68 0.80 1.31 5.17 2.29 5.18 0.18 3.52
Nuclear Periphery 1.64 -5.93 -1.05 -3.77 5.67 4.13 -2.25 2.45 -2.54 3.15 3.77 -5.90 0.91 -4.42 6.15
Nuclear Periphery Foci 1.60 -5.19 -2.74 -3.80 3.71 4.97 -2.30 2.88 -3.30 2.40 4.54 -5.46 -0.50 -4.81 5.34
Nucleolus 3.24 -5.98 0.16 -1.46 5.01 5.86 0.07 6.01 0.38 3.82 6.63 -3.34 5.43 -1.15 7.47
Nucleus 2.36 -12.67 -2.96 -4.22 7.68 4.81 -3.91 5.52 0.47 4.57 5.20 -11.61 1.82 -3.08 12.54
Peroxisomes -5.72 -2.20 -12.53 -8.55 -11.71 -6.48 -6.06 -9.79 -2.89 2.74 -8.66 -3.77 -15.67 -7.85 -12.52
Vacuole -0.08 -30.80 -12.21 -12.06 7.88 2.02 -7.02 -10.32 -11.58 3.97 1.39 -30.84 -16.43 -16.92 13.13
Vacuole Periphery 2.06 -5.19 2.06 0.40 6.21 8.90 3.69 4.42 -4.78 -0.81 8.25 -1.49 3.97 -3.35 5.27
Standard name
Human Ortholog
Description Mitochondrial inner membrane protein; binds to the 5' UTR of the COX3 mRNA to activate its translation together with Pet122p and Pet494p; also binds to the COX1 Group I intron AI5 beta to facilitate exon ligation during splicing
Localization
Cell Percentages mitochondrion (91%)
Cell Cycle Regulation No
Subcompartmental Group mito-1

Pet54

Pet54


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Pet54-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available