Standard name | |
---|---|
Human Ortholog | |
Description | Protein that negatively regulates mitotic exit; forms a complex with Kel1p and Bud14p that regulates Bnr1p (formin) to affect actin cable assembly, cytokinesis, and polarized growth; functions in a complex with Kel1p, interacts with Tem1p and Lte1p; localizes to regions of polarized growth; potential Cdc28p substrate |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 30 | 33 | 31 | 13 | 35 | 20 | 10 | 10 | 12 | 9 | 9 | 9 | 23 | 11 | 12 | 17 | 42 | 63 | 27 | 25 | 25 | 16 | 31 | 36 |
Bud | 22 | 18 | 26 | 7 | 36 | 29 | 27 | 18 | 18 | 16 | 16 | 18 | 22 | 24 | 20 | 10 | 22 | 34 | 33 | 30 | 31 | 45 | 77 | 66 |
Bud Neck | 3 | 1 | 23 | 8 | 26 | 8 | 15 | 13 | 5 | 9 | 4 | 8 | 4 | 1 | 0 | 1 | 16 | 1 | 36 | 31 | 41 | 60 | 51 | 26 |
Bud Site | 12 | 14 | 13 | 10 | 8 | 16 | 12 | 13 | 6 | 6 | 11 | 1 | 17 | 2 | 4 | 8 | 22 | 15 | – | – | – | – | – | – |
Cell Periphery | 1 | 0 | 5 | 1 | 2 | 2 | 1 | 1 | 3 | 2 | 1 | 1 | 6 | 10 | 18 | 5 | 9 | 14 | 2 | 3 | 3 | 1 | 4 | 6 |
Cytoplasm | 81 | 69 | 123 | 62 | 128 | 129 | 161 | 139 | 96 | 75 | 85 | 70 | 133 | 146 | 157 | 22 | 42 | 37 | 28 | 47 | 64 | 16 | 30 | 62 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 2 | 1 | 3 | 0 | 1 | 1 | 2 | 2 | 4 | 5 | 1 | 0 | 0 | 4 | 1 | 1 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 4 | 3 | 3 | 3 | 1 | 3 | 6 | 3 | 3 | 4 | 3 | 4 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 3 | 1 | 2 | 0 | 0 | 0 | 0 | 2 | 1 |
Mitochondria | 24 | 22 | 4 | 6 | 1 | 26 | 22 | 17 | 55 | 37 | 68 | 42 | 5 | 2 | 2 | 4 | 9 | 14 | 13 | 14 | 24 | 15 | 22 | 14 |
Nucleus | 1 | 1 | 5 | 0 | 3 | 5 | 2 | 4 | 4 | 2 | 1 | 7 | 0 | 3 | 1 | 6 | 10 | 7 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 2 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 3 | 0 | 0 | 0 | 0 | 0 | 1 | 0 |
Peroxisomes | 6 | 0 | 0 | 0 | 0 | 2 | 0 | 1 | 1 | 0 | 1 | 0 | 10 | 10 | 8 | 6 | 10 | 12 | 1 | 1 | 1 | 1 | 1 | 3 |
SpindlePole | 12 | 5 | 28 | 11 | 35 | 26 | 32 | 21 | 8 | 6 | 11 | 8 | 17 | 5 | 5 | 14 | 30 | 21 | 4 | 7 | 11 | 4 | 4 | 4 |
Vac/Vac Membrane | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 2 | 0 | 1 | 1 | 0 | 4 | 0 | 0 | 2 | 3 | 1 | 3 | 4 | 4 | 5 | 5 | 4 |
Unique Cell Count | 143 | 125 | 190 | 94 | 196 | 201 | 225 | 192 | 168 | 121 | 158 | 117 | 194 | 187 | 200 | 72 | 143 | 139 | 161 | 175 | 221 | 180 | 245 | 237 |
Labelled Cell Count | 194 | 163 | 260 | 118 | 274 | 264 | 283 | 240 | 210 | 165 | 211 | 164 | 248 | 218 | 232 | 103 | 224 | 229 | 161 | 175 | 221 | 180 | 245 | 237 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 3.8 | 3.8 | 4.8 | 4.4 | 5.1 | 3.9 | 4.3 | 4.4 | 3.4 | 3.4 | 3.2 | 3.8 | 4.8 | 4.9 | 4.7 | 8.8 | 8.2 | 8.2 | 4.2 | 4.3 | 4.7 |
Std Deviation (1e-4) | 0.6 | 0.5 | 1.2 | 1.3 | 2.0 | 1.3 | 1.9 | 1.8 | 1.2 | 1.2 | 0.9 | 1.2 | 2.3 | 1.6 | 1.2 | 2.8 | 2.0 | 1.8 | 1.3 | 1.8 | 1.9 |
Intensity Change (Log2) | – | – | – | -0.13 | 0.1 | -0.29 | -0.15 | -0.1 | -0.49 | -0.48 | -0.59 | -0.32 | 0.03 | 0.03 | -0.0 | 0.88 | 0.79 | 0.78 | -0.17 | -0.13 | -0.0 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Neck | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Site | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cell Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cyto | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endoplasmic Reticulum | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endosome | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Golgi | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Mitochondria | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nuclear Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nuc | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleolus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Peroxisomes | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
SpindlePole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Vac | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cortical Patches | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cytoplasm | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Vacuole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 2.0036 | 1.6613 | 1.8734 | 1.8081 | 1.6189 | 1.958 | 0.1254 | -0.0681 | 0.0501 | -0.3868 | -0.2306 | -0.0851 | -0.3699 | -0.3225 | -0.4206 | -0.2884 | 0.1987 | -0.4978 |
Actin | 0.0355 | 0.0087 | 0.0167 | 0.0616 | 0.0031 | 0.0169 | 0.0763 | 0.0118 | 0.0553 | 0.0148 | 0.0849 | 0.0491 | 0.0372 | 0.0133 | 0.0127 | 0.007 | 0.058 | 0.015 |
Bud | 0.0305 | 0.0255 | 0.1277 | 0.0238 | 0.0205 | 0.0047 | 0.0454 | 0.0326 | 0.1837 | 0.0832 | 0.0469 | 0.0069 | 0.0399 | 0.052 | 0.1377 | 0.0614 | 0.0535 | 0.0174 |
Bud Neck | 0.088 | 0.0075 | 0.0125 | 0.0029 | 0.1488 | 0.538 | 0.1007 | 0.0048 | 0.0013 | 0.0026 | 0.0016 | 0.5344 | 0.043 | 0.0062 | 0.0053 | 0.0018 | 0.0008 | 0.4066 |
Bud Periphery | 0.092 | 0.0335 | 0.3971 | 0.2841 | 0.2577 | 0.0241 | 0.1193 | 0.0625 | 0.4829 | 0.4863 | 0.2875 | 0.0229 | 0.087 | 0.0641 | 0.2911 | 0.1945 | 0.2425 | 0.0385 |
Bud Site | 0.154 | 0.1877 | 0.0354 | 0.0011 | 0.0285 | 0.0075 | 0.1988 | 0.2464 | 0.0267 | 0.0055 | 0.0092 | 0.0129 | 0.1892 | 0.3083 | 0.0706 | 0.0159 | 0.0108 | 0.019 |
Cell Periphery | 0.0171 | 0.0073 | 0.0055 | 0.0145 | 0.0082 | 0.0053 | 0.0206 | 0.0103 | 0.0024 | 0.0101 | 0.005 | 0.0039 | 0.0087 | 0.0068 | 0.0037 | 0.0044 | 0.0036 | 0.0046 |
Cytoplasm | 0.1219 | 0.0875 | 0.0322 | 0.0846 | 0.0504 | 0.1577 | 0.1391 | 0.1538 | 0.0356 | 0.1369 | 0.096 | 0.1336 | 0.0629 | 0.0738 | 0.0426 | 0.0631 | 0.0199 | 0.1032 |
Cytoplasmic Foci | 0.021 | 0.0287 | 0.0118 | 0.0036 | 0.0463 | 0.0073 | 0.0368 | 0.0102 | 0.018 | 0.0109 | 0.0309 | 0.0463 | 0.0583 | 0.0189 | 0.0243 | 0.0065 | 0.0142 | 0.0528 |
Eisosomes | 0.003 | 0.0017 | 0.0009 | 0.003 | 0.001 | 0.0008 | 0.0021 | 0.0012 | 0.0007 | 0.0004 | 0.0018 | 0.0006 | 0.0014 | 0.0005 | 0.0005 | 0.0009 | 0.0007 | 0.0005 |
Endoplasmic Reticulum | 0.0069 | 0.001 | 0.0013 | 0.0024 | 0.0015 | 0.0013 | 0.0067 | 0.0032 | 0.0005 | 0.0033 | 0.0017 | 0.0012 | 0.0041 | 0.0009 | 0.0021 | 0.0048 | 0.0021 | 0.0012 |
Endosome | 0.0149 | 0.0015 | 0.0026 | 0.0006 | 0.0205 | 0.0016 | 0.0227 | 0.001 | 0.0092 | 0.0426 | 0.0053 | 0.011 | 0.01 | 0.0008 | 0.0051 | 0.0008 | 0.0077 | 0.0014 |
Golgi | 0.0041 | 0.0006 | 0.0018 | 0.0012 | 0.0013 | 0.001 | 0.0131 | 0.0005 | 0.0156 | 0.0043 | 0.0041 | 0.0053 | 0.0034 | 0.0005 | 0.0009 | 0.0003 | 0.0031 | 0.001 |
Lipid Particles | 0.0069 | 0.0009 | 0.0018 | 0.0007 | 0.0075 | 0.0008 | 0.0079 | 0.0007 | 0.0036 | 0.0147 | 0.0112 | 0.0045 | 0.0127 | 0.0005 | 0.0031 | 0.0008 | 0.008 | 0.0009 |
Mitochondria | 0.0026 | 0.0013 | 0.007 | 0.004 | 0.0028 | 0.0012 | 0.0175 | 0.0011 | 0.0179 | 0.0023 | 0.0473 | 0.0027 | 0.0089 | 0.0026 | 0.0098 | 0.0031 | 0.0292 | 0.0029 |
None | 0.3466 | 0.5975 | 0.3289 | 0.5063 | 0.3846 | 0.219 | 0.1587 | 0.4506 | 0.1321 | 0.1654 | 0.3259 | 0.142 | 0.3792 | 0.4078 | 0.3199 | 0.5675 | 0.5052 | 0.292 |
Nuclear Periphery | 0.0127 | 0.0003 | 0.0014 | 0.0004 | 0.0004 | 0.0049 | 0.0076 | 0.0005 | 0.0003 | 0.0008 | 0.0008 | 0.0048 | 0.0084 | 0.0014 | 0.0029 | 0.0056 | 0.0081 | 0.001 |
Nucleolus | 0.0025 | 0.0011 | 0.0009 | 0.0008 | 0.0057 | 0.0002 | 0.0014 | 0.0013 | 0.0004 | 0.0023 | 0.0005 | 0.0007 | 0.0031 | 0.0038 | 0.0074 | 0.0047 | 0.0035 | 0.0021 |
Nucleus | 0.0134 | 0.0012 | 0.0023 | 0.0008 | 0.0015 | 0.0008 | 0.0022 | 0.0025 | 0.0007 | 0.0019 | 0.0011 | 0.002 | 0.0047 | 0.0176 | 0.0201 | 0.0337 | 0.0081 | 0.0101 |
Peroxisomes | 0.0122 | 0.0017 | 0.0085 | 0.0005 | 0.0031 | 0.0055 | 0.0139 | 0.0006 | 0.0107 | 0.001 | 0.0334 | 0.0114 | 0.024 | 0.0023 | 0.0058 | 0.0006 | 0.0067 | 0.0111 |
Punctate Nuclear | 0.0066 | 0.0011 | 0.0009 | 0.0003 | 0.0024 | 0.0002 | 0.0013 | 0.0013 | 0.0014 | 0.001 | 0.0011 | 0.0022 | 0.009 | 0.0154 | 0.0304 | 0.017 | 0.0085 | 0.0158 |
Vacuole | 0.0065 | 0.0033 | 0.0026 | 0.0023 | 0.0036 | 0.0008 | 0.0055 | 0.0029 | 0.0009 | 0.009 | 0.0031 | 0.001 | 0.0037 | 0.0023 | 0.0035 | 0.0051 | 0.0037 | 0.0026 |
Vacuole Periphery | 0.0011 | 0.0005 | 0.0004 | 0.0003 | 0.0004 | 0.0004 | 0.0025 | 0.0002 | 0.0003 | 0.0009 | 0.0008 | 0.0005 | 0.0011 | 0.0003 | 0.0006 | 0.0007 | 0.002 | 0.0004 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 11.8713 | 4.8407 | 4.1446 | 7.578 | 9.9911 | 7.4935 | 6.2121 | 9.4019 | 9.4175 | 14.3058 |
Translational Efficiency | – | – | – | – | – | – | – | – | – | – |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | – |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
2145 | 1595 | 410 | 1604 | 1567 | 1891 | 271 | 598 | 3712 | 3486 | 681 | 2202 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 663.40 | 748.90 | 987.57 | 849.78 | 673.40 | 738.80 | 894.80 | 811.00 | 667.62 | 743.42 | 950.65 | 839.25 |
Standard Deviation | 67.13 | 91.83 | 112.32 | 112.42 | 107.50 | 94.68 | 110.95 | 113.10 | 86.64 | 93.52 | 120.65 | 113.92 |
Intensity Change Log 2 | — | 0.174894 | 0.574004 | 0.357210 | — | 0.133720 | 0.410102 | 0.268238 | — | 0.154300 | 0.493767 | 0.313077 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.005346 | 0.004762 | 0.040935 | 0.006268 | 0.006118 | 0.006424 | 0.006774 | 0.008449 | 0.005672 | 0.005664 | 0.027341 | 0.006860 |
Bud Neck | 0.271251* | 0.157655 | 0.114832 | 0.122244 | 0.202667* | 0.221249* | 0.123322 | 0.053269 | 0.242299* | 0.192152* | 0.118211 | 0.103512 |
Bud Site | 0.214499* | 0.207287* | 0.402804* | 0.302463* | 0.206870* | 0.185327* | 0.303687* | 0.201221* | 0.211278* | 0.195375* | 0.363361* | 0.274968* |
Cell Periphery | 0.002941 | 0.000972 | 0.004205 | 0.001286 | 0.002679 | 0.002039 | 0.002931 | 0.001101 | 0.002830 | 0.001551 | 0.003698 | 0.001236 |
Cytoplasm | 0.295134* | 0.300186* | 0.098159 | 0.257404* | 0.301580* | 0.269054* | 0.127946* | 0.330282* | 0.297855* | 0.283298* | 0.110012* | 0.277196* |
Cytoplasmic Foci | 0.089288 | 0.145754 | 0.039487 | 0.020184 | 0.129419 | 0.134361 | 0.023663 | 0.069699 | 0.106229 | 0.139574 | 0.033190 | 0.033631 |
Eisosomes | 0.002255 | 0.000586 | 0.001050 | 0.000256 | 0.002309 | 0.001456 | 0.000484 | 0.000419 | 0.002278 | 0.001058 | 0.000825 | 0.000300 |
Endoplasmic Reticulum | 0.000519 | 0.000617 | 0.015218 | 0.002581 | 0.000893 | 0.000953 | 0.008783 | 0.001436 | 0.000677 | 0.000799 | 0.012657 | 0.002270 |
Endosome | 0.010319 | 0.022248 | 0.006668 | 0.004355 | 0.013984 | 0.022251 | 0.004047 | 0.006151 | 0.011866 | 0.022250 | 0.005625 | 0.004843 |
Golgi | 0.004262 | 0.007788 | 0.000730 | 0.003258 | 0.005287 | 0.009260 | 0.000281 | 0.000600 | 0.004695 | 0.008587 | 0.000552 | 0.002536 |
Lipid Particles | 0.005026 | 0.003834 | 0.006068 | 0.001003 | 0.008595 | 0.004745 | 0.003548 | 0.002476 | 0.006533 | 0.004328 | 0.005065 | 0.001403 |
Mitochondria | 0.007897 | 0.011901 | 0.001481 | 0.003125 | 0.009137 | 0.022598 | 0.000650 | 0.000686 | 0.008421 | 0.017704 | 0.001150 | 0.002463 |
Mitotic Spindle | 0.001928 | 0.004938 | 0.003719 | 0.018244 | 0.000983 | 0.004206 | 0.002776 | 0.005176 | 0.001529 | 0.004541 | 0.003343 | 0.014695 |
None | 0.001401 | 0.000643 | 0.002617 | 0.000759 | 0.001427 | 0.000835 | 0.000473 | 0.003426 | 0.001412 | 0.000747 | 0.001764 | 0.001483 |
Nuclear Periphery | 0.000064 | 0.000083 | 0.000517 | 0.000378 | 0.000111 | 0.000083 | 0.000552 | 0.000384 | 0.000084 | 0.000083 | 0.000531 | 0.000380 |
Nuclear Periphery Foci | 0.000084 | 0.000144 | 0.002713 | 0.000691 | 0.000212 | 0.000144 | 0.001216 | 0.000721 | 0.000138 | 0.000144 | 0.002117 | 0.000699 |
Nucleolus | 0.000287 | 0.000523 | 0.000447 | 0.000227 | 0.000405 | 0.000469 | 0.000353 | 0.000375 | 0.000337 | 0.000494 | 0.000410 | 0.000267 |
Nucleus | 0.037790 | 0.036678 | 0.109010 | 0.091050 | 0.046303 | 0.032477 | 0.192904* | 0.112981 | 0.041384 | 0.034399 | 0.142395* | 0.097005 |
Peroxisomes | 0.011430 | 0.027411 | 0.019396 | 0.005525 | 0.017514 | 0.027351 | 0.010252 | 0.021702 | 0.013998 | 0.027379 | 0.015757 | 0.009918 |
Vacuole | 0.037422 | 0.064593 | 0.129680 | 0.156526 | 0.042368 | 0.052899 | 0.185092 | 0.179212 | 0.039510 | 0.058250 | 0.151731 | 0.162687 |
Vacuole Periphery | 0.000855 | 0.001395 | 0.000263 | 0.002175 | 0.001138 | 0.001818 | 0.000268 | 0.000234 | 0.000975 | 0.001625 | 0.000265 | 0.001648 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | 0.68 | -6.15 | 0.52 | -0.23 | 6.26 | -0.29 | -0.17 | -0.59 | -0.34 | -0.41 | 0.04 | -6.00 | 0.32 | 0.27 | 6.07 |
Bud Neck | 12.96 | 15.44 | 24.08 | 10.57 | 2.00 | -1.87 | 8.25 | 19.22 | 23.02 | 4.82 | 7.60 | 17.06 | 28.81 | 21.73 | 3.80 |
Bud Site | 1.07 | -7.24 | 1.32 | 0.28 | 7.72 | 2.37 | -1.64 | 4.91 | 3.44 | 4.52 | 2.72 | -6.79 | 3.28 | 1.05 | 8.29 |
Cell Periphery | 7.38 | -1.75 | 7.63 | 0.32 | 6.24 | 2.08 | 0.69 | 6.77 | 7.39 | 5.51 | 6.34 | -1.27 | 9.70 | 4.65 | 7.69 |
Cytoplasm | -0.61 | 18.71 | 3.06 | 3.46 | -15.47 | 3.59 | 12.68 | 0.30 | -2.23 | -10.21 | 2.26 | 22.44 | 3.00 | 1.03 | -18.11 |
Cytoplasmic Foci | -9.91 | 10.34 | 20.57 | 26.39 | 5.71 | -0.80 | 20.03 | 10.38 | 11.43 | -7.15 | -8.09 | 20.14 | 24.46 | 31.14 | 1.15 |
Eisosomes | 7.99 | 5.38 | 10.12 | 5.57 | 5.88 | 2.30 | 5.23 | 5.58 | 8.64 | 2.40 | 6.09 | 7.39 | 10.75 | 10.71 | 6.48 |
Endoplasmic Reticulum | -1.02 | -10.21 | -10.37 | -10.19 | 8.65 | -0.30 | -9.42 | -6.11 | -5.08 | 8.29 | -1.11 | -12.81 | -11.26 | -10.16 | 10.81 |
Endosome | -7.10 | 3.62 | 9.53 | 12.08 | 5.68 | -4.91 | 8.85 | 6.83 | 10.71 | -1.69 | -8.87 | 8.44 | 12.30 | 17.10 | 4.08 |
Golgi | -5.02 | 11.47 | 3.70 | 6.92 | -1.97 | -5.17 | 11.39 | 10.83 | 13.83 | -2.91 | -7.54 | 16.09 | 6.51 | 11.52 | -2.22 |
Lipid Particles | 1.76 | -2.38 | 7.01 | 5.78 | 8.62 | 3.66 | 3.65 | 5.41 | 3.78 | 2.64 | 3.77 | 0.78 | 9.00 | 8.82 | 9.30 |
Mitochondria | -2.95 | 7.36 | 6.26 | 8.67 | -1.35 | -6.79 | 8.29 | 8.34 | 12.97 | 0.25 | -7.55 | 10.91 | 9.43 | 14.73 | -1.66 |
Mitotic Spindle | -2.23 | -1.43 | -5.25 | -3.23 | -4.06 | -3.60 | -1.90 | -3.46 | -1.70 | -1.23 | -3.72 | -2.40 | -6.35 | -3.95 | -4.01 |
None | 3.12 | -0.49 | 1.79 | -3.13 | 0.75 | 2.15 | 2.51 | -1.62 | -2.30 | -2.45 | 3.54 | -0.20 | -0.29 | -2.86 | 0.12 |
Nuclear Periphery | -1.60 | -11.62 | -13.21 | -12.76 | 0.76 | 1.29 | -8.99 | -10.44 | -11.49 | 1.65 | -0.00 | -14.64 | -15.86 | -16.08 | 1.52 |
Nuclear Periphery Foci | -4.33 | -8.05 | -7.73 | -6.93 | 6.20 | 1.34 | -4.54 | -9.08 | -10.35 | 2.24 | -0.59 | -9.19 | -10.00 | -9.89 | 6.54 |
Nucleolus | -2.07 | -2.67 | 2.92 | 2.73 | 3.87 | -0.83 | 0.74 | -0.11 | 0.70 | -0.76 | -2.33 | -1.78 | 2.48 | 3.35 | 3.37 |
Nucleus | 0.23 | -9.41 | -20.81 | -20.30 | 0.50 | 5.09 | -11.60 | -10.04 | -12.32 | 6.26 | 3.66 | -14.54 | -22.70 | -24.64 | 4.40 |
Peroxisomes | -8.33 | -1.89 | 6.85 | 12.75 | 4.46 | -4.55 | 2.37 | -0.06 | 2.93 | -1.90 | -9.40 | -0.15 | 5.23 | 13.00 | 2.94 |
Vacuole | -9.15 | -12.81 | -32.62 | -26.98 | -7.38 | -3.40 | -13.61 | -20.13 | -19.23 | -2.33 | -9.06 | -18.43 | -38.18 | -34.24 | -6.63 |
Vacuole Periphery | -3.08 | 4.53 | -0.86 | 0.29 | -2.17 | -3.87 | 8.19 | 8.80 | 11.41 | 1.74 | -5.11 | 8.16 | 0.02 | 1.91 | -2.13 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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