Standard name
Human Ortholog
Description TFIIIB B-related factor; one of three subunits of RNA polymerase III transcription initiation factor TFIIIB, binds TFIIIC and TBP and recruits RNA pol III to promoters, amino-terminal half is homologous to TFIIB; mutations in human homolog are associated with autosomal recessive cerebellar-facial-dental syndrome
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 – – 0 0 0 – 0 0 0 0 – – 0 0 0 0 0 0 0 0 0 0
Bud 0 0 – – 0 0 0 – 0 0 0 0 – – 0 0 0 0 0 0 0 0 0 0.06
Bud Neck 0 0 – – 0 0 0 – 0 0 0 0 – – 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 – – 0 0 0 – 0 0 0 0.05 – – 0 0 0 0 – – – – – –
Cell Periphery 0 0 – – 0 0 0 – 0 0 0 0 – – 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 – – 0.09 0.05 0.07 – 0 0.05 0 0 – – 0.05 0.06 0 0.1 0 0 0 0.07 0 0
Endoplasmic Reticulum 0 0 – – 0 0 0 – 0 0 0 0 – – 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 – – 0 0 0 – 0 0 0 0 – – 0.1 0 0 0 0 0 0 0 0 0
Golgi 0 0 – – 0 0 0 – 0 0 0 0 – – 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.14 0 – – 0 0.11 0.13 – 0.24 0.22 0.46 0.21 – – 0 0.06 0.05 0.05 0 0 0 0 0 0
Nucleus 0.78 0.9 – – 0.89 0.82 0.7 – 0.78 0.65 0.6 0.38 – – 0.71 0.79 0.82 0.77 0.64 0.85 0.9 0.68 0.73 0.76
Nuclear Periphery 0 0 – – 0 0 0 – 0 0 0 0 – – 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.29 0.15 – – 0 0.16 0.25 – 0.28 0.14 0.18 0.69 – – 0.05 0.11 0.12 0.1 0.18 0.08 0.05 0.16 0.11 0.1
Peroxisomes 0 0 – – 0 0 0 – 0 0 0 0 – – 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 – – 0 0 0 – 0 0 0 0 – – 0.05 0 0 0 0 0 0 0 0 0.05
Vac/Vac Membrane 0 0 – – 0 0 0 – 0 0 0 0 – – 0.14 0 0 0.07 0.05 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 – – 0 0 0 – 0 0 0 0 – – 0 0 0 0 0 0 0 0 0 0
Bud 1 0 – – 0 0 0 – 0 0 1 0 – – 0 1 0 0 1 0 0 0 0 2
Bud Neck 0 0 – – 0 0 0 – 0 0 1 0 – – 0 0 0 0 0 1 0 0 0 0
Bud Site 1 0 – – 0 0 1 – 0 0 2 2 – – 0 1 0 0 – – – – – –
Cell Periphery 0 0 – – 0 0 0 – 0 0 0 0 – – 0 0 0 0 0 0 0 0 0 0
Cytoplasm 6 3 – – 4 3 8 – 1 2 3 1 – – 1 15 8 15 0 0 0 0 0 0
Endoplasmic Reticulum 1 0 – – 0 0 0 – 0 0 0 0 – – 0 0 4 5 1 0 0 0 0 0
Endosome 0 2 – – 0 1 0 – 0 0 0 0 – – 2 2 2 2 3 3 0 0 0 0
Golgi 0 0 – – 0 0 0 – 0 0 0 0 – – 0 1 0 0 3 1 0 0 0 0
Mitochondria 23 2 – – 0 6 14 – 16 8 31 8 – – 0 15 11 8 5 0 0 0 1 0
Nucleus 131 192 – – 41 45 75 – 53 24 41 15 – – 15 190 174 119 113 194 26 10 18 29
Nuclear Periphery 0 0 – – 0 0 0 – 0 0 0 0 – – 0 2 1 2 0 0 0 0 0 0
Nucleolus 49 33 – – 2 9 27 – 19 5 12 27 – – 1 27 25 15 32 19 1 2 2 3
Peroxisomes 0 0 – – 0 0 0 – 0 0 0 0 – – 0 0 1 0 0 0 0 0 0 0
SpindlePole 0 0 – – 2 0 4 – 0 0 0 1 – – 1 3 2 0 1 0 0 0 0 1
Vac/Vac Membrane 4 4 – – 0 0 2 – 0 1 1 0 – – 3 9 6 11 9 6 0 0 0 1
Unique Cell Count 167 214 46 55 107 68 37 68 39 21 240 211 154 178 230 30 15 25 39
Labelled Cell Count 216 236 49 64 131 89 40 92 54 23 266 234 177 178 230 30 15 25 39


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.7 6.9 – – 6.7 5.6 5.5 – 4.7 5.1 5.3 5.1 – – 7.2 8.8 8.8 8.7 – – –
Std Deviation (1e-4) 1.0 1.0 – – 1.5 1.5 1.6 – 0.9 1.5 1.6 1.2 – – 1.7 1.9 1.6 1.9 – – –
Intensity Change (Log2) – – – – – – – – – – – – – – – – – – – – –


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 1.4896 1.8719 1.6971 0.9687 1.4803 1.4807 1.9491 2.0867 2.0233 1.6032 1.6623 1.7719 1.6455 1.8572 1.4165 1.3054 0.6948 1.2379
Actin 0.0115 0 0.0022 0.0079 0.0075 0.0055 0.0368 0 0.0129 0.0014 0.0001 0.0024 0.0155 0.0014 0.0153 0.0021 0.0022 0.0062
Bud 0.0004 0 0.0005 0.0001 0.0004 0.0001 0.0005 0 0.0007 0.0002 0.0001 0.0001 0.0005 0.0002 0.0011 0.0005 0.0006 0.0002
Bud Neck 0.0011 0.0001 0.0006 0.0004 0.0017 0.0014 0.0029 0.0001 0.0017 0.0003 0.0005 0.0007 0.0015 0.0015 0.0012 0.0002 0.0007 0.0011
Bud Periphery 0.0015 0.0001 0.0008 0.0002 0.0012 0.0001 0.0012 0.0001 0.0017 0.0004 0.0003 0.0001 0.0007 0.0005 0.0019 0.0007 0.0005 0.0003
Bud Site 0.0011 0.0001 0.0012 0.0006 0.0011 0.0002 0.0065 0.0001 0.0105 0.0006 0.0004 0.0001 0.0061 0.0074 0.0094 0.0037 0.0007 0.0002
Cell Periphery 0.0004 0 0.0002 0 0.0002 0 0.0006 0 0.0009 0.0002 0.0001 0 0.0004 0.0004 0.0005 0.0002 0.0001 0.0001
Cytoplasm 0.0042 0.0001 0.0018 0.0308 0.0045 0.001 0.0031 0.0001 0.005 0.0004 0.0046 0.0012 0.0121 0.0002 0.0122 0.0029 0.0011 0.0003
Cytoplasmic Foci 0.0159 0 0.0035 0.0124 0.0063 0.0122 0.0194 0 0.0204 0.0015 0.0119 0.0028 0.0102 0.0016 0.0202 0.0241 0.0284 0.003
Eisosomes 0.0002 0 0.0001 0.0001 0.0001 0 0.0013 0 0.0006 0.0001 0 0 0.0001 0 0.0001 0 0 0
Endoplasmic Reticulum 0.001 0 0.0013 0.0023 0.0005 0.0006 0.0031 0 0.0025 0.0017 0.0005 0.0034 0.0016 0.0003 0.0038 0.0051 0.0004 0.0004
Endosome 0.0225 0.0001 0.0068 0.0225 0.0168 0.0073 0.0274 0 0.0169 0.0223 0.059 0.0078 0.0097 0.0037 0.0325 0.0591 0.0581 0.0083
Golgi 0.0118 0 0.0021 0.0089 0.0074 0.0094 0.0069 0 0.0078 0.0063 0.0041 0.0037 0.0052 0.0013 0.0075 0.0044 0.0247 0.0087
Lipid Particles 0.0259 0 0.0093 0.0312 0.0428 0.0133 0.0251 0 0.0296 0.0025 0.0015 0.0093 0.0221 0.0018 0.0126 0.0108 0.0698 0.0112
Mitochondria 0.02 0.0005 0.0207 0.0043 0.0135 0.0027 0.0097 0.0002 0.0087 0.0274 0.0009 0.0106 0.0044 0.016 0.0578 0.0036 0.0616 0.0112
None 0.0086 0 0.0024 0.0221 0.0138 0.0001 0.0074 0 0.0205 0.0002 0.0002 0.0003 0.0056 0.0001 0.0025 0.0007 0.0004 0.0001
Nuclear Periphery 0.0076 0.0073 0.0107 0.0202 0.0072 0.0007 0.0417 0.002 0.0069 0.0012 0.0014 0.0069 0.0018 0.0042 0.006 0.0106 0.0013 0.0009
Nucleolus 0.0489 0.0498 0.0504 0.0462 0.1557 0.1155 0.0217 0.0391 0.0461 0.0589 0.1599 0.0598 0.044 0.0428 0.0433 0.0238 0.1511 0.0614
Nucleus 0.762 0.9263 0.8752 0.7601 0.6377 0.8222 0.7401 0.9569 0.7677 0.8631 0.7483 0.8848 0.8213 0.9075 0.7491 0.8005 0.5409 0.881
Peroxisomes 0.0314 0 0.0012 0.0088 0.0064 0.0033 0.0264 0 0.0162 0.0002 0.0003 0.0034 0.0251 0.0016 0.0099 0.0337 0.0354 0.0016
Punctate Nuclear 0.0176 0.0151 0.0041 0.0189 0.0695 0.0036 0.0109 0.0009 0.011 0.006 0.0032 0.0015 0.0096 0.0038 0.0022 0.0024 0.0164 0.0028
Vacuole 0.004 0.0001 0.0026 0.001 0.0044 0.0003 0.0042 0.0001 0.0099 0.0033 0.0021 0.0005 0.0021 0.0029 0.0081 0.0084 0.0031 0.0005
Vacuole Periphery 0.0025 0.0002 0.0024 0.0009 0.0012 0.0002 0.0029 0.0002 0.0019 0.0018 0.0007 0.0005 0.0005 0.0009 0.0025 0.0022 0.0024 0.0005

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 22.179 13.2178 14.3036 19.3447 18.7713 16.0093 19.2114 18.9798 21.5803 21.5522
Translational Efficiency 1.815 1.9872 1.907 1.4496 1.5023 1.9984 1.2837 1.2328 1.1531 1.3217

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
209 1277 759 1841 1825 1501 1436 606 2034 2778 2195 2447

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 667.04 848.59 1026.34 957.20 786.05 817.03 995.34 964.55 773.82 831.54 1006.06 959.02
Standard Deviation 88.27 122.20 134.41 152.83 102.41 114.72 125.49 160.71 107.31 119.26 129.49 154.85
Intensity Change Log 2 — 0.347294 0.621664 0.521047 — 0.055768 0.340568 0.295235 — 0.196935 0.476431 0.403297

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000523 0.000661 0.001183 0.001261 0.000257 0.000466 0.000845 0.001596 0.000284 0.000555 0.000962 0.001344
Bud Neck 0.008131 0.010047 0.025705 0.035703 0.005801 0.012676 0.023840 0.032795 0.006040 0.011468 0.024485 0.034983
Bud Site 0.001722 0.002033 0.001880 0.006954 0.000393 0.002797 0.001384 0.013934 0.000530 0.002446 0.001555 0.008682
Cell Periphery 0.000130 0.000058 0.000046 0.000159 0.000037 0.000093 0.000056 0.000190 0.000047 0.000077 0.000052 0.000167
Cytoplasm 0.025799 0.003796 0.001000 0.010937 0.002303 0.002431 0.000788 0.006792 0.004717 0.003058 0.000861 0.009910
Cytoplasmic Foci 0.004093 0.000698 0.000030 0.002016 0.000372 0.001002 0.000106 0.000923 0.000755 0.000862 0.000079 0.001745
Eisosomes 0.000056 0.000028 0.000048 0.000033 0.000020 0.000034 0.000043 0.000038 0.000024 0.000032 0.000044 0.000034
Endoplasmic Reticulum 0.003566 0.002691 0.003385 0.005711 0.001419 0.003151 0.002752 0.004178 0.001640 0.002940 0.002971 0.005331
Endosome 0.001226 0.000663 0.000145 0.002501 0.000500 0.001473 0.000321 0.004583 0.000575 0.001101 0.000260 0.003017
Golgi 0.000529 0.000337 0.000179 0.001481 0.000385 0.000757 0.000210 0.000896 0.000400 0.000564 0.000199 0.001336
Lipid Particles 0.002172 0.000204 0.000073 0.001356 0.000203 0.000585 0.000044 0.000739 0.000405 0.000410 0.000054 0.001204
Mitochondria 0.004026 0.002386 0.001374 0.003488 0.001384 0.004796 0.001163 0.009611 0.001655 0.003688 0.001236 0.005004
Mitotic Spindle 0.009578 0.005210 0.002250 0.024221 0.001913 0.005437 0.003356 0.021907 0.002700 0.005332 0.002973 0.023648
None 0.027397 0.004856 0.001724 0.007291 0.005181 0.004256 0.002776 0.015087 0.007464 0.004532 0.002412 0.009222
Nuclear Periphery 0.001087 0.000542 0.000215 0.002675 0.000443 0.000783 0.000242 0.002366 0.000509 0.000672 0.000233 0.002599
Nuclear Periphery Foci 0.003438 0.001162 0.000104 0.001968 0.001075 0.000581 0.000129 0.001312 0.001318 0.000848 0.000120 0.001805
Nucleolus 0.113458 0.110946 0.060644 0.021960 0.118234 0.104680 0.068012 0.028077 0.117743 0.107560 0.065464 0.023475
Nucleus 0.788554* 0.851318* 0.898101* 0.862413* 0.859289* 0.848040* 0.892918* 0.846929* 0.852021* 0.849547* 0.894711* 0.858578*
Peroxisomes 0.002265 0.000869 0.000966 0.000481 0.000466 0.001292 0.000490 0.001214 0.000651 0.001097 0.000655 0.000662
Vacuole 0.001613 0.001066 0.000757 0.005936 0.000147 0.002973 0.000372 0.005098 0.000298 0.002096 0.000505 0.005729
Vacuole Periphery 0.000635 0.000430 0.000190 0.001455 0.000177 0.001697 0.000154 0.001733 0.000224 0.001115 0.000166 0.001524

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -1.28 -8.72 -7.79 -8.51 0.09 -4.14 -19.15 -10.44 -9.33 -6.16 -6.88 -22.53 -16.49 -13.21 -5.37
Bud Neck -0.87 -9.55 -13.04 -15.79 -4.26 -8.05 -20.20 -12.67 -9.74 -3.13 -8.28 -24.44 -22.89 -18.49 -5.84
Bud Site 0.46 -0.49 -3.52 -4.03 -4.04 -3.16 -12.11 -4.43 -3.26 -3.81 -3.42 -9.48 -8.27 -5.14 -6.29
Cell Periphery 3.09 3.26 0.33 -4.86 -5.37 -2.79 -2.28 -8.23 -2.67 -7.18 -2.37 -0.92 -7.67 -3.95 -7.58
Cytoplasm 2.83 3.29 2.20 -3.02 -6.42 -0.09 2.54 -2.30 -2.11 -3.83 1.39 4.11 -2.34 -4.18 -7.52
Cytoplasmic Foci 1.93 2.17 1.82 -0.79 -3.39 -1.65 2.39 -0.07 1.52 -1.85 0.37 2.92 0.35 -0.03 -3.62
Eisosomes 4.72 1.42 3.46 -4.76 5.68 -6.60 -18.01 -10.89 -4.74 -0.85 -3.98 -15.09 -8.94 -5.22 4.78
Endoplasmic Reticulum 1.44 0.13 -3.32 -10.66 -7.40 -7.18 -11.79 -9.47 -5.84 -6.05 -7.26 -11.17 -16.33 -11.48 -10.62
Endosome 1.84 4.02 -0.21 -2.11 -4.41 -2.10 0.75 -1.90 -0.78 -2.28 -1.51 1.28 -2.58 -1.40 -4.54
Golgi 1.36 2.03 -0.72 -1.55 -1.88 -1.29 1.15 -0.67 0.55 -1.59 -0.77 1.37 -1.25 -0.74 -2.22
Lipid Particles 2.81 2.94 1.89 -1.25 -1.49 -1.17 3.08 -1.89 0.08 -3.11 0.24 3.95 -0.71 -0.79 -1.94
Mitochondria 1.37 2.36 0.53 -1.55 -8.55 -3.25 -0.07 -2.91 -0.73 -3.00 -2.72 0.39 -4.33 -0.71 -6.43
Mitotic Spindle 0.81 1.25 -2.56 -5.86 -6.75 -2.06 -2.35 -6.53 -5.70 -5.81 -2.17 -1.63 -9.76 -8.31 -8.96
None 3.38 3.87 3.21 -1.61 -7.98 1.17 4.04 -2.08 -2.52 -3.38 3.13 5.87 0.56 -3.17 -6.90
Nuclear Periphery 2.06 3.67 -4.14 -6.89 -8.66 -1.50 0.09 -4.73 -3.96 -4.96 -1.17 1.08 -8.23 -7.75 -9.75
Nuclear Periphery Foci 2.30 3.24 2.13 -0.49 -3.88 1.30 2.14 -0.14 -2.33 -3.96 1.32 2.86 0.20 -1.84 -5.06
Nucleolus 1.09 4.18 8.44 19.18 10.18 2.76 8.43 18.93 15.76 9.54 2.64 10.00 24.23 25.70 14.63
Nucleus -4.27 -5.68 -4.72 -1.16 2.53 1.58 -3.00 2.32 1.29 3.99 0.19 -4.98 -0.26 -0.47 4.48
Peroxisomes 2.67 2.13 3.44 2.07 6.15 -2.12 -0.32 -1.49 1.09 -1.54 -1.62 -0.55 0.84 2.40 2.50
Vacuole 1.14 1.13 -1.48 -5.17 -5.97 -4.16 -6.91 -5.48 -3.64 -5.24 -3.41 -1.62 -8.50 -5.68 -8.29
Vacuole Periphery 0.83 1.87 -0.66 -1.57 -2.52 -3.87 0.51 -2.14 1.67 -2.19 -4.08 1.12 -3.08 0.94 -3.32
Standard name
Human Ortholog
Description TFIIIB B-related factor; one of three subunits of RNA polymerase III transcription initiation factor TFIIIB, binds TFIIIC and TBP and recruits RNA pol III to promoters, amino-terminal half is homologous to TFIIB; mutations in human homolog are associated with autosomal recessive cerebellar-facial-dental syndrome
Localization
Cell Percentages nucleus (98%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-4

Brf1

Brf1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Brf1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available